Documentation

AnalyzeMicroscopeOverlay

AnalyzeMicroscopeOverlay[microscopeImage]⟹overlayObject

overlays microscope image data from multiple fluorescence and phase contrast channels to create a single combined image.

Details

Input

Output

Brightness Options

Contrast Options

FalseColor Options

Others Options

Method Options

Examples

Basic Examples (4)

Overlay images from one Microscope data:

Analyze all data from a Microscope protocol:

Analyze a list of Microscope data:

Analyze all data from a list of Microscope protocols:

Additional Examples (4)

Overlay a microscope image containing Red, Green, and Blue channels:

Overlay a microscope image containing Red and Green channels:

Overlay a microscope image containing only Green channel:

Analyze all data from a list of mixed Microscope protocol (one without data, one with data, and one data object):

Options (18)

BrightnessBlue (1)

Adjust brightness of the FalseBlue channel:

BrightnessGreen (1)

Adjust brightness of the FalseGreen channel:

BrightnessRed (1)

Adjust brightness of the FalseRed channel:

ChannelsOverlaid (1)

Only overlay Red channel:

ContrastBlue (1)

Adjust contrast of the FalseBlue channel:

ContrastGreen (1)

Adjust contrast of the FalseGreen channel:

ContrastRed (1)

Adjust contrast of the FalseRed channel:

FalseColorBlue (1)

Adjust color base of the FalseBlue channel:

FalseColorGreen (1)

Adjust color base of the FalseRed channel:

FalseColorRed (1)

Adjust color base of the Green and Red channels:

Output (4)

Return the packet:

Return the options:

Return the preview:

Return the tests:

Template (3)

Use options from previous MicroscopeOverlay analysis:

Explicitly specify Transparency option, and pull remaining options from previous MicroscopeOverlay analysis:

Explicitly specify different options for a list of inputs:

Transparency (1)

Increase transparency of the fluorescent image, making phase contrast image more visible:

Messages (4)

InvalidOverlayChannels (1)

The specified channels must be available in the data object:

NoData (1)

Provided Protocols must include at least one data:

NoFluorescentChannel (1)

PhaseContrast cannot be only channel specified:

NoOverlayChannels (1)

There must be at least one overlay channel specified/available: