Coupling

Coupling[couplingRules]⟹primitive

generates an solid phase synthesis primitive that describes the reagents and conditions to be used for monomer activation via formation of a reactive esters and coupling of that monomer to the strand being synthesized.

Details

When using the Coupling primitive, the activating solution can be specified as a single Activator solution, or two seperate Preactivator and Base solutions.
The following keys can be used as inputs to the primitive:

Key	Value Pattern	Description
Monomer	ObjectP[{Object[Sample],Model[Sample],Object[Sample],Model[Sample,StockSolution]}]	The monomer solution which will be activated via formation of a reactive ester.
MonomerVolume	GreaterP[0 Microliter]	The volume of monomer solution used.
Activator	ObjectP[{Object[Sample],Model[Sample],Object[Sample],Model[Sample,StockSolution]}]	The solution which will be used to perform the activation of the monomer.
ActivatorVolume	GreaterP[0 Microliter]	The volume of activator solution used.
Preactivator	ObjectP[{Object[Sample],Model[Sample],Object[Sample],Model[Sample,StockSolution]}]	The solution which, in conjunction with the Base solution, will be used to perform the activation of the monomer.
PreactivatorVolume	GreaterP[0 Microliter]	The volume of preactivator solution used.
Base	ObjectP[{Object[Sample],Model[Sample],Object[Sample],Model[Sample,StockSolution]}]	The solution which, in conjunction with the Preactivator solution, will be used to perform the activation of the monomer.
BaseVolume	GreaterP[0 Microliter]	The volume of base solution used.
ActivationTime	GreaterP[0 Second]	The amount of time for which the monomer will be mixed in the activation solution(s).
CouplingTime	GreaterP[0 Second]	The amount of time for which the activated monomer will be coupled to the the resin.