ecl-logo Documentation

Deprotonating

Deprotonating[deprotonationRules]⟹primitive

generates an solid phase synthesis primitive that describes how a proton is removed from a strand during solid phase synthesis.

Details

  • The following keys can be used as inputs to the primitive:
  • Key
    Value Pattern
    Description
    SampleObjectP[{Object[Sample],Model[Sample],Object[Sample],Model[Sample,StockSolution]}]The solution which will be used to perform the deprotonation.
    TimeGreaterP[0 Second]The amount of time for which the deprotonation solution will be mixed with the resin.
    VolumeGreaterP[0 Microliter]The volume of deprotonation solution used.
    NumberOfReplicatesGreaterP[0]Number of times the deprotonation step will be repeated.
    • Input
    Output
    Attributes
  • Protected

Examples

Basic Examples  (3)

Create a primitve to deprotonate the strand during the synthesis:

The sample provided to the primitive must have a liquid state:

Sample, Time, Volume and NumberOfReplicates keys are all required:

Attributes  (1)

Protected  (1)

The Deprotecting Head is protected:

EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.