ECL`

ExperimentMeasureConductivity

ExperimentMeasureConductivity[Samples]⟹Protocol

generates a Protocol to measure conductivity of the provided Samples.

Electrical conductivity meter (EC meter) measures electrical conductivity in µS/cm by immersing conductivity probe in a solution. The EC meter applies an alternating voltage at an optimal frequency on the probe sensor and measures the size of the resulting signal (depending on the probe type), which is directly related to the conductivity. During this process, the cations migrate to the negative electrode, the anions to the positive electrode and the solution acts as an electrical conductor. The more free anions and cations the liquid contains, the higher the electrical conductivity. Conductivity measurement is a rapid and inexpensive way to monitor the ionic content in a solution, for instance the measurement of the amount of nutrients, salts or impurities in water.

Experimental Principles

Figure 1.1: Figure 1.0: Procedural overview of a MeasureConductivity experiment. Step 1: During the first step, the conductivity Probe is calibrated. Step 2: Based on the specifications, the probe is primed with a rinse sample before testing, if RinseSample->True.Step 3: Probe is then introduced into the sample and electrical conductivity is measured. Step 4: After the final sample measurement, the Probe is cleaned and stored.

Instrumentation

SevenExcellence Conductivity

Figure 2.1: The conductivity probe is immersed into the sample.Depending on the customer's needs there are two different types of the probe available at the ECL facilities. AmperometricSmallSampleVolume (2 electrodes) probe is suitable for the low volume samples (150 uL minimum).Potentiometric Regular (4 electrodes) probes have higher accuracy and wider conductivity range but require minimum of 5 mL sample volume.Conductivity meter applies an alternating voltage on the probe and measures the resulting signal:amperometric or potentiometric,depending on the probe type.Taking into account probe calibration the resulting signal transforms to the electrical conductivity output readings.

Experiment Options

General

Instrument

The conductivity meter which communicates with the probe in order to take conductivity measurements.

Default Value: Model[Instrument, ConductivityMeter, SevenExcellence Conductivity]

Pattern Description: An object of type or subtype Object[Instrument, ConductivityMeter] or Model[Instrument, ConductivityMeter]

Programmatic Pattern: ObjectP[{Object[Instrument, ConductivityMeter], Model[Instrument, ConductivityMeter]}]

Probe

For each sample, the conductivity probe which is immersed in each sample for conductivity measurement.

Default Value: Automatic

Default Calculation: Automatically set to the 4 electrode probe will be chosen automatically if there is more than 5 mL, otherwise, it will pick the 2 electrode probe.

Pattern Description: An object of type or subtype Model[Part, ConductivityProbe]

Programmatic Pattern: ObjectP[{Model[Part, ConductivityProbe]}] | Automatic

Index Matches to: experiment samples

NumberOfReadings

For each sample, the number of times the conductivity of each aliquot or sample will be read by taking another recording.

Default Value: 3

Pattern Description: Greater than or equal to 1 and less than or equal to 10 in increments of 1.

Programmatic Pattern: RangeP[1, 10, 1]

Index Matches to: experiment samples

NumberOfReplicates

The number of times sample will be additionally aliquoted to the different container to obtain additional measurements.

Default Value: Null

Pattern Description: Greater than or equal to 2 and less than or equal to 10 in increments of 1 or Null.

Programmatic Pattern: RangeP[2, 10, 1] | Null

Calibration

CalibrationStandard

When measuring the conductivity standard the probe cell constant value is adjusted until the measured conductivity value matches the CalibrationConductivity option.

Default Value: Automatic

Default Calculation: Automatically set to Model[Sample, "Conductivity Standard 1413 µS, Sachets"]. If CalibrationConductivity is specified, automatically set to the nearest available calibration standard.

Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample.

Programmatic Pattern: (ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic

CalibrationConductivity

The probe cell constant is adjusted to match the CalibrationConductivity when measuring CalibrationStandard.

Default Value: Automatic

Default Calculation: Resolves to the conductivity of the CalibrationStandard model's Conductivity field. If the specified value does not have a matching calibration standard sample available, automatically set to the nearest available calibration standard sample's Conductivity.

Pattern Description: Greater than or equal to 10 microsiemens per centimeter and less than or equal to 1000 millisiemens per centimeter.

Programmatic Pattern: RangeP[10*Micro*(Siemens/Centimeter), 1000*Milli*(Siemens/Centimeter)] | Automatic

VerificationStandard

Standard used to test the calibration by insuring that VerificationConductivity of the VerificationStandard is correct. If verification fails the failure will be recorded in the protocol object VerificationResult field, and measurement will proceed regardless.

Default Value: Automatic

Default Calculation: Automatically set to Model[Sample, "Conductivity Standard 84"]. If VerificationConductivity is specified, automatically set to the nearest available verification standard.

Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample.

Programmatic Pattern: (ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic

VerificationConductivity

The VerificationStandard is used to test the calibration by ensuring that it matches the VerificationConductivity.If verification fails the failure will be recorded in the protocol object VerificationResult field, and measurement will proceed regardless.

Default Value: Automatic

Default Calculation: Automatically set to the conductivity of the VerificationStandard model's Conductivity field. If the specified value does not have a matching verification standard sample available, automatically set to the nearest available verification standard sample's Conductivity.

Pattern Description: Greater than or equal to 10 microsiemens per centimeter and less than or equal to 1000 millisiemens per centimeter.

Programmatic Pattern: RangeP[10*Micro*(Siemens/Centimeter), 1000*Milli*(Siemens/Centimeter)] | Automatic

TemperatureCompensation

TemperatureCorrection

For each sample, the relationship between temperature and conductivity, such as Linear, NonLinear, None, or PureWater, which is optimized type of temperature algorithm. Linear should be used for the temperature correction of medium and highly conductive solutions. NonLinear should be used for natural water for temperature between 0-36° C. PureWater should be used for the pure water measurements. None and Off won't use any temperature compensation algorithms, and returns measured conductivity at measured temperature.

Default Value: Automatic

Default Calculation: Automatically set to Linear. If AlphaCoefficient is specified as Null set to None.

Pattern Description: Linear, NonLinear, None, PureWater, or Off.

Programmatic Pattern: TemperatureCorrectionP | Automatic

Index Matches to: experiment samples

AlphaCoefficient

For each sample, the relative change of conductivity(%) that is associated with a given change in temperature (°C).

Default Value: Automatic

Default Calculation: Automatically set to 2 if TemperatureCorrection is Linear and Null otherwise.

Pattern Description: Greater than or equal to 0 and less than or equal to 10 in increments of 1 or Null.

Programmatic Pattern: (RangeP[0, 10, 1] | Automatic) | Null

Index Matches to: experiment samples

Preparation

SampleRinse

For each sample, the True/False value indicates if the probe will be rinsed with the input sample prior to measuring its conductivity.

Default Value: False

Pattern Description: True or False.

Programmatic Pattern: BooleanP

Index Matches to: experiment samples

SampleRinseVolume

For each sample, the volume of a sample that should be transferred from the input samples into a fresh container used for rinsing the probe.

Default Value: Automatic

Default Calculation: Automatically set to the minimum volume required for the measurements with the conductivity probe: 5 mL for the 4 electrode probe, 150 uL for the 2 electrode probe.

Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: (RangeP[1*Microliter, 20*Liter] | Automatic) | Null

Index Matches to: experiment samples

SampleRinseStorageCondition

For each sample, the condition under which the samples used to rinse the probe are stored after use.

Default Value: Automatic

Default Calculation: Automatically set to the Disposal if SampleRinse is True.

Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting}, Disposal, or Null.

Programmatic Pattern: (SampleStorageTypeP | Disposal | Null) | Automatic

Index Matches to: experiment samples

Post Processing

RecoupSample

For each sample, the True/False value indicates if the aliquots used for conductivity measurement will be transferred back into the original container after conductivity measurement.

Default Value: False

Pattern Description: True or False.

Programmatic Pattern: BooleanP

Index Matches to: experiment samples

Model Input

PreparedModelContainer

Indicates the container in which a Model[Sample] specified as input to the experiment function will be prepared.

Default Value: Automatic

Default Calculation: If PreparedModelAmount is set to All and when the input model has a product associated with both Amount and DefaultContainerModel populated, automatically set to the DefaultContainerModel value in the product. Otherwise set to Model[Container, Vessel, "2mL Tube"].

Pattern Description: An object of type or subtype Model[Container] or Null.

Programmatic Pattern: (ObjectP[Model[Container]] | Automatic) | Null

Index Matches to: experiment samples

PreparedModelAmount

Indicates the amount of a Model[Sample] specified as input to the experiment function that will be prepared in the PreparedModelContainer. When set to All and the input model sample is not preparable, the entire amount of the input model sample that comes from one of the Products is prepared. The selected product must have both Amount and DefaultContainerModel populated, and it must not be a KitProduct. When set to All and the input model is preparable such as water, 1 Milliliter of the input model sample is prepared.

Default Value: Automatic

Default Calculation: Automatically set to 1 Milliliter.

Pattern Description: All or Count or Count or Mass or Volume or Null.

Index Matches to: experiment samples

Post Experiment

SamplesInStorageCondition

The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.

Default Value: Null

Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null

Index Matches to: experiment samples

Sample Prep Options

Sample Preparation

PreparatoryUnitOperations

Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSamplePreparation.

Default Value: Null

Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.

Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null

Preparatory Incubation

Incubate

Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

IncubationTemperature

Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.

Default Value: Automatic

Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.

Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null

Index Matches to: experiment samples

IncubationTime

Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

Mix

Indicates if this sample should be mixed while incubated, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

MixType

Indicates the style of motion used to mix the sample, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on the container of the sample and the Mix option.

Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.

Programmatic Pattern: (MixTypeP | Automatic) | Null

Index Matches to: experiment samples

MixUntilDissolved

Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

MaxIncubationTime

Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.

Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

IncubationInstrument

The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.

Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.

Programmatic Pattern: (ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]] | Automatic) | Null

Index Matches to: experiment samples

AnnealingTime

Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquotContainer

The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquotDestinationWell

The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquot

The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

Preparatory Centrifugation

Centrifuge

Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

CentrifugeInstrument

The centrifuge that will be used to spin the provided samples prior to starting the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null

Index Matches to: experiment samples

CentrifugeIntensity

The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.

Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeTime

The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than 0 minutes or Null.

Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null

Index Matches to: experiment samples

CentrifugeTemperature

The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.

Default Value: Automatic

Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.

Programmatic Pattern: ((Ambient | RangeP[-10*Celsius, 40*Celsius]) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquotContainer

The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquotDestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquot

The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

Preparatory Filtering

Filtration

Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

FiltrationType

The type of filtration method that should be used to perform the filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.

Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.

Programmatic Pattern: (FiltrationTypeP | Automatic) | Null

Index Matches to: experiment samples

FilterInstrument

The instrument that should be used to perform the filtration.

Default Value: Automatic

Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.

Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null

Index Matches to: experiment samples

Filter

The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.

Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.

Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null

Index Matches to: experiment samples

FilterMaterial

The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.

Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, AgaroseResin, CobaltResin, Silica, HLB, or AnoporeAlumina or Null.

Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null

Index Matches to: experiment samples

PrefilterMaterial

The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.

Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null

Index Matches to: experiment samples

FilterPoreSize

The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.

Pattern Description: 0.008 micrometers, 0.02 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.

Programmatic Pattern: (FilterSizeP | Automatic) | Null

Index Matches to: experiment samples

PrefilterPoreSize

The pore size of the filter; all particles larger than this should be removed during the filtration.

Default Value: Automatic

Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.

Programmatic Pattern: (FilterSizeP | Automatic) | Null

Index Matches to: experiment samples

FilterSyringe

The syringe used to force that sample through a filter.

Default Value: Automatic

Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.

Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

FilterHousing

The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.

Default Value: Automatic

Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.

Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null

Index Matches to: experiment samples

FilterIntensity

The rotational speed or force at which the samples will be centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.

Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null

Index Matches to: experiment samples

FilterTime

The amount of time for which the samples will be centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than 0 minutes or Null.

Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null

Index Matches to: experiment samples

FilterTemperature

The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than or equal to 4 degrees Celsius or Null.

Programmatic Pattern: ((Alternatives[GreaterEqualP[4*Celsius]]) | Automatic) | Null

Index Matches to: experiment samples

FilterContainerOut

The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.

Default Value: Automatic

Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.

Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.

Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

FilterAliquotDestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

FilterAliquotContainer

The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

FilterAliquot

The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

FilterSterile

Indicates if the filtration of the samples should be done in a sterile environment.

Default Value: Automatic

Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

Aliquoting

Aliquot

Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).

Default Value: Automatic

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

AliquotAmount

The amount of a sample that should be transferred from the input samples into aliquots.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.

Pattern Description: All or Count or Count or Mass or Volume or Null.

Index Matches to: experiment samples

TargetConcentration

The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Default Calculation: Automatically calculated based on aliquot and buffer volumes.

Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.

Programmatic Pattern: ((GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)]) | Automatic) | Null

Index Matches to: experiment samples

TargetConcentrationAnalyte

The substance whose final concentration is attained with the TargetConcentration option.

Default Value: Automatic

Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.

Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.

Programmatic Pattern: (ObjectP[IdentityModelTypes] | Automatic) | Null

Index Matches to: experiment samples

AssayVolume

The desired total volume of the aliquoted sample plus dilution buffer.

Default Value: Automatic

Default Calculation: Automatically determined based on Volume and TargetConcentration option values.

Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: (RangeP[1*Microliter, 20*Liter] | Automatic) | Null

Index Matches to: experiment samples

ConcentratedBuffer

The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

BufferDilutionFactor

The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.

Pattern Description: Greater than or equal to 1 or Null.

Programmatic Pattern: (GreaterEqualP[1] | Automatic) | Null

Index Matches to: experiment samples

BufferDiluent

The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

AssayBuffer

The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

AliquotSampleStorageCondition

The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.

Default Value: Automatic

Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null

Index Matches to: experiment samples

DestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.

Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

AliquotContainer

The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.

Default Value: Automatic

Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.

Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.

Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

AliquotPreparation

Indicates the desired scale at which liquid handling used to generate aliquots will occur.

Default Value: Automatic

Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.

Pattern Description: Manual or Robotic or Null.

Programmatic Pattern: (PreparationMethodP | Automatic) | Null

ConsolidateAliquots

Indicates if identical aliquots should be prepared in the same container/position.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Protocol Options

Organizational Information

Template

A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.

Default Value: Null

Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.

Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

Name

A object name which should be used to refer to the output object in lieu of an automatically generated ID number.

Default Value: Null

Pattern Description: A string or Null.

Programmatic Pattern: _String | Null

Post Experiment

MeasureWeight

Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

MeasureVolume

Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

ImageSample

Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Example Calls

Contacting probe

To measure with a particular conductivity probe, choose the Probe option:

Temperature compensation algorithm, such as Linear, NonLinear, PureWater, or None can be defined:

Linear temperature correction can be controlled by adjusting alpha coefficient that describes the relative change of conductivity(%) that is associated with a given change in temperature (°C):

The probe calibration and verification standards can be defined in order to control the quality assurance:

Aliquoted sample defined by SampleRinse option can be used to rinse the probe prior to the measurement to increase accuracy at low conductivity:

Aliquoted sample defined by Aliquot->True can be recouped to the original container by defining Recoup Sample option:

Preferred Input Containers

8x43mm Glass Reaction Vial

New 0.5mL Tube with 2mL Tube Skirt

2mL Tube

15mL Tube

50mL Tube

Warnings and Errors

Messages (20)

ConductivityStandardUnknowConductivity (1)

Return an error the Conductivity of the given CalibrationStandard is unknown:

ConductivityVerificationStandardUnknowConductivity (1)

Return an error if the Conductivity of the given VerificationStandard is unknown:

ConflictingCalibrantion (1)

Return an error if the given CalibrationConductivity does not match with the Conductivity of CalibrationStandard:

ConflictingTemperatureCorrection (1)

Return an error if the given TemperatureCorrection conflicts with teh AlphaCoefficient:

ConflictingVerification (1)

Return an error if the given VerificationConductivity does not match with the Conductivity of VerificationStandard:

DiscardedSamples (1)

Return an error for a discarded sample:

EmptyContainers (1)

Return an error for a container without a sample:

IncompatibleSampleConductivity (1)

Return a warning if the sample has a conductivity value outside any available conductivity range:

IncompatibleSampleConductivityProbe (1)

Return a warning the conductivity of the sample is incompatible with any available probe:

InsufficientSampleRinseVolume (1)

Return an error if InsufficientSampleRinseVolume is given:

InsufficientVolume (1)

Return an error for too low sample volume, thereby incapable of measurement:

NoSuitableProbe (1)

Return a warning if the sample has a conductivity value outside any available conductivity range of the small volume probe:

ObjectDoesNotExist (4)

Throw a message if we have a container that does not exist (ID form):

Throw a message if we have a container that does not exist (name form):

Throw a message if we have a sample that does not exist (ID form):

Throw a message if we have a sample that does not exist (name form):

SampleRinseStorageConditionMismatch (2)

Return an error for SampleRinseStorageCondition specified when SampleRinse is turened off:

SolidSamples (1)

Return an error for a solid sample:

VolumeUnknown (1)

Return an error for samples that do not have a volume:

Possible Issues

Minimum Sample Volumes

Each conductivity probe requires the fluid volume of the measure reach a minimum fill volume height. This minimum height is dependent on the probe model and the assay container model. When measuring conductance of low volume samples, the best containers to use are Model[Container, Vessel, "id:4pO6dM5WvJKM"], which requires 475uL of sample, or Model[Container, Vessel, "id:o1k9jAG00e3N"], which required 675uL of sample.

Last modified on Tue 9 Sep 2025 11:29:57