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Incubate

Incubate[sample]⟹primitive

generates an ExperimentSampleManipulation-compatible primitive that describes incubating and mixing a sample at a specified temperature and shaking rate for a specified amount of time.

Details

  • Incubate can be performed on both micro and macro liquid handling scale.
  • Time and Temperature are required fields for micro liquid handling scale, MixRate is optional.
  • MixRate, ResidualIncubation, ResidualTemperature, ResidualMix, and ResidualMixRate are optional keys not specific to either scale.
  • Preheat is an optional keys specific to micro liquid handling. If specified, the scale will resolve to micro, if possible. Do not specify these keys if MacroLiquidHandling scale is desired.
  • MixType,Instrument, NumberOfMixes, MixVolume, MixUntilDissolved, MaxNumberOfMixes, MaxTime and AnnealingTime are optional keys specific to macro liquid handling. If specified, the scale will resolve to macro, if possible. Do not specify these keys if MicroLiquidHandling scale is desired.
  • On the macro liquid handling scale, ResidualMixRate and ResidualTemperature must be identical to MixRate and Temperature, if specified. If ResidualMixRate and/or ResidualTemperature are specified, while MixRate and/or Temperature are left empty, MixRate and Temperature will automatically resolve to the values specified in ResidualMixRate an/or ResidualTemperature. If mixing is occurring, and ResidualIncubation is set to True, then ResidualMix cannot be set to False. Reversely, if ResidualMix is set to True, ResidualIncubation cannot be set to False.
  • If no scale-specific key is specified, the liquid handling scale will resolve to micro liquid handling, unless LiquidHandlingScale is specified to MacroLiquidHandling or other manipulations require macro.
  • For incubating on macro liquid handling scale, the resolution of keys that are not specified is identical to the option resolution behavior in ExperimentIncubate.
    • Input
    Output
    Pipetting Options
    General Options
    Attributes
  • Protected

Examples

Basic Examples  (3)

Create a primitive to specify incubating a sample for 1 Minute at 40 Celsius:

Specify that the Sample should be mixed while incubating:

Specify a final temperature to hold the sample(s) at after the specified incubation Time has elapsed:

Additional Examples  (10)

All Incubate fields are listable allowing multiple sources to be incubated simultaneously:

If a singleton field value is specified, it will be applied to all sources:

MacroLiquidHandling  (4)

Specify that the sample(s) should stay inside the incubation device after the heater has been switched off:

Specify that the sample incubation should be continued (MixUntilDissolved) up to MaxTime in an attempt dissolve any solute:

Specify the instrument that should be used to incubate and mix the sample:

Specify that the sample should be mixed by Inversion:

MicroLiquidHandling  (4)

Specify a final rate to mix the sample(s) after the specified Time has elapsed:

Specify that the heating block should be brought to the specified temperature before any samples are exposed to it:

Specify that the sample(s) should be held at the specified incubation Temperature after Time has elapsed:

Specify that the sample(s) should be held at the specified MixRate after Time has elapsed:

Attributes  (1)

Protected  (1)

The Incubate head is protected:

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