UploadCapillaryELISACartridge

UploadCapillaryELISACartridge[analytes]⟹capillaryELISACartridgeModel

creates a new pre-loaded capillaryELISACartridgeModel with the specified analytes, cartridge type and species.

Details

The pre-loaded capillary ELISA cartridge are used in ExperimentCapillaryELISA for Enzyme-Linked Immunosorbent Assay (ELISA) experiments. A pre-loaded cartridge of ExperimentCapillaryELISA is loaded with capture antibodies (embedded on the bottom of the glass nano-reactors), detection antibodies and streptavidin-conjugated fluorophore reagents, providing the complete assay of the entire ELISA workflow of the specific analytes.
The cartridge comes with factory-calibrated standard curves of the assays, validated by the assay developer. A standard curve of an analyte was fit from standard sample data using the five-parameter logistic model: FluorescenceSignal = D + (A - D) / (1 + (Concentration / C) ^ B) ^ G Fluorescence is provided in unit RFU and Concentration is provided in unit Picogram/Milliliter.
There are four types of pre-loaded capillary ELISA cartridges. The cartridges are pre-loaded with validated ELISA assays, available for more than 200 analytes. Different pre-loaded cartridge types provide different sample numbers, analyte numbers and internal replicates:

Cartridge Type	Max Number of Samples	Max Number of Analytes	Internal Replicates per Sample
SinglePlex72X1	72	1	3
MultiAnalyte32X4	32	4	3
MultiAnalyte16X4	16	4	3
MultiPlex32X8	32	8	2

Please refer to Object[Report,Literature,"Simple Plex Assay Menu"] for the available analytes of pre-loaded cartridges and Object[ManufacturingSpecification,CapillaryELISACartridge] of different analytes for compatibility and combination rules.
Due to the manufacturing and validation steps, the lead time of a pre-loaded cartridge assay can take up to 14 days. After the new capillary ELISA cartridge model is created by UploadCapillaryELISACartridge, it is recommended to use OrderSamples to order the cartridge ahead to reduce the waiting time of the experiment.

Create a model for a new SinglePlex72X1 capillary ELISA cartridge from an identity model:

Create a model for a new SinglePlex72X1 capillary ELISA cartridge:

Create a model for a new MultiAnalyte32X4 capillary ELISA cartridge from identity models:

Create a model for a new MultiPlex32X8 capillary ELISA cartridge from analyte names:

The created cartridge model is valid:

The CartridgeType option can be used to select the type of capillary ELISA cartridge:

The Species option can be specified to select the organism of samples for this cartridge:

Returns the existing cartridge model from an analyte molecule if it is already available:

Returns the existing cartridge model from an analyte name if it is already available:

Returns the existing MultiAnalyte32X4 cartridge model from analyte names if it is already available: