Indicate whether the absorbance spectrum should be blanked:

Indicate what the blank(s) should be for this experiment:

Indicate what the blank(s) should be for this experiment, expanded for multiple samples:

If BlankAbsorbance -> True and Blanks is not specified, resolve to Milli-Q water:

If BlankAbsorbance -> False and Blanks is not specified, resolve to Null:

Indicate the volume of blank to use for each sample:

Indicate the volume of blank to use for each sample, using multiple samples:

If BlankAbsorbance -> True, BlankVolumes is not specified, and Instrument -> a BMG plate reader, resolve BlankVolumes to the volume of the corresponding sample:

If BlankAbsorbance -> True, BlankVolumes is not specified, and Instrument -> a Lunatic, resolve BlankVolumes to 2 uL:

If BlankAbsorbance -> False and BlankVolumes is not specified, resolve BlankVolumes to Null:

Rounds specified BlankVolumes to the nearest 0.1 microliter:

Specify the time for which the plates of samples should be equilibrated at the Temperature:

If using the Lunatic, EquilibrationTime resolves to Null:

If using the Lunatic, EquilibrationTime resolves to 0*Minute:

Rounds specified EquilibrationTime to the nearest second:

Indicate that the ContainersIn should be imaged after absorbance readings:

If not specified, ImageSample resolves to True:

Specify which plate reader instrument will be used during the protocol:

Provide a name for the protocol:

If OutputFormat -> TestSummary, return a test summary:

Use PreparatoryPrimitives option to create test standards prior to running the experiment:

Indicate that the concentration of the input samples should be calculated:

If QuantifyConcentration is not specified but Wavelength is specified, resolve option to False:

If QuantifyConcentration is not specified and Wavelength is also not specified, resolve option to False:

Specify the temperature at which the plates should be read in the plate reader:

Rounds specified Temperature to the nearest 0.1 degree Celsius:

If using the Lunatic, Temperature resolves to Null:

If using a BMG plate reader, Temperature resolves to Ambient:

Set the Template option:

Inherit the resolved options of a previous protocol:

If Verbose -> Failures, return the results of all failing tests and warnings:

Indicate the wavelength of the quantification that should be calculated:

If QuantifyConcentration is not specified and Wavelength is also not specified, resolve option to 260 Nanometer:

If QuantifyConcentration is True and Wavelength is not specified, resolve option to 260 Nanometer IF the ExtinctionCoefficients field is not populated in any sample's model (and throw a warning):

If QuantifyConcentration is True and Wavelength is not specified, resolve option to the first value stored in the sample's model's ExtinctionCoefficients field:

Rounds specified Wavelength to the nearest nanometer:

If the specified Blanks option contains samples that are also inputs, throw an error and return $Failed:

Returns an error if using the Lunatic and BlankVolumes is anything but 2.1*Microliter:

Throw an error if one or multiple of the input samples are discarded:

If the specified Name option already exists as a protocol object, throw an error and return $Failed:

If sample concentration is being quantified, the extinction coefficient should be populated in the sample model:

Returns an error if BlankAbsorbance -> True and Blanks -> Null:

Returns an error if BlankAbsorbance -> True and BlankVolumes -> Null:

Returns an error if BlankAbsorbance -> False and Blanks is specified:

Returns an error if BlankAbsorbance -> False and BlankVolumes is specified:

Returns an error if QuantifyConcentration -> True and BlankAbsorbance -> False:

If QuantificationAnalyte is specified, it must be a component of the input sample:

Returns an error if the Temperature option is specified with an instrument that does not support it:

Returns an error if the EquilibrationTime option is specified with an instrument that does not support it:

If the combination of NumberOfReplicates * (Number of samples + number of unique blanks) is greater than 94 and we are using the Lunatic, throw an error:

Returns an error if the specified plate reader model is deprecated:

Returns an error if the specified plate reader object is retired: