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ValidExperimentFPLCQ

ValidExperimentFPLCQ[objects]bools

checks whether the provided objects and specified options are valid for calling ExperimentFPLC.

Details

    Input
    Output
    Aliquoting Options
    Blanks Options
    ColumnFlush Options
    ColumnPrime Options
    FractionCollection Options
    Gradient Options
    Organizational Information Options
    Post Experiment Options
    Preparatory Centrifugation Options
    Preparatory Filtering Options
    Preparatory Incubation Options
    Sample Parameters Options
    Sample Preparation Options
    Standard Options
    General Options

Examples

Basic Examples  (2)

Generates and returns a protocol object when given samples:

Generates and returns a protocol object when given one unlisted sample:

Options  (2)

OutputFormat  (1)

Toggle the output format of the tests to output a TestSummary:

Verbose  (1)

Display all tests (or test failures) with the Verbose option:

Messages  (33)

ConductivityThresholdNotSupported  (1)

If using non-avant instruments, cannot use conductivity threshold values:

ConflictFractionOptionSpecification  (1)

If CollectFractions -> False and other fraction collection options are specified, a warning is thrown:

ConflictingFractionCollectionOptions  (1)

Both threshold and peak fraction collection options may not be specified together:

ConflictingPeakSlopeOptions  (2)

All or none of the peak fraction collections must be specified together

If specified, the peak fraction options must have compatible units:

ContainerlessSamples  (1)

Samples without containers cannot be used as inputs

DiscardedSamples  (1)

Discarded samples cannot be used as inputs:

DuplicateName  (1)

If the Name option is specified, it must not be the name of an already-existing FPLC protocol:

FlowRateAboveMax  (1)

If the specified FlowRate is above the allowed value for the provided Column, an error is thrown:

FPLCTooManySamples  (3)

If too many samples were specified to perform in one experiment (including blanks and standards), throw an error:

If too many samples were specified to perform in one experiment (including blanks and standards), throw an error:

If one of the samples/blanks/standards is injecting too much volume to use on the autosampler, the maximum number of samples is only 5:

FractionVolumeAboveMax  (1)

If using Model[Instrument, FPLC, "AKTApurifier UPC 10"], MaxFractionVolume cannot be greater than 2 Milliliter:

GradientStartEndConflict  (1)

GradientStart and GradientEnd must either both be specified or both blank:

IncompatibleColumnTechnique  (1)

Non-FPLC columns cannot be specified:

IncompatibleInjectionVolume  (1)

The specified injection volumes must be at or below the maximum allowed injection volume for the provided instrument:

InjectionTableBlankConflict  (1)

If the Blank option is specified, it must agree with the values specified in the InjectionTable:

InjectionTableBlankFrequencyConflict  (1)

BlankFrequency and InjectionTable may not be specified together:

InjectionTableColumnGradientConflict  (1)

If the InjectionTable option is specified, only one ColumnFlush and one ColumnPrime gradient is allowed:

InjectionTableForeignBlanks  (1)

If the InjectionTable is specified and its values do not agree with the blank values, an error is thrown:

InjectionTableForeignSamples  (1)

If the InjectionTable is specified, the samples included in it must match the input samples precisely:

InjectionTableForeignStandards  (1)

If the InjectionTable is specified and its values do not agree with the standard values, an error is thrown:

InjectionTableGradientConflict  (1)

The InjectionTable and Gradient options, if both specified, must agree with each other:

InjectionTableStandardConflict  (1)

If the Standard option is specified, it must agree with the values specified in the InjectionTable:

InjectionTableStandardFrequencyConflict  (1)

StandardFrequency and InjectionTable may not be specified together:

InstrumentDoesNotContainDetector  (1)

The specified detectors must be available on the specified instrument:

InvalidFractionCollectionEndTime  (1)

FractionCollectionEndTime may not be longer than the duration of the gradient:

InvalidFractionCollectionTemperature  (1)

If the FractionCollectionTemperature cannot be set to the specified value for the provided instrument, an error is thrown:

InvalidGradientComposition  (1)

If a Gradient is specified manually, its percentages must sum to 100%:

InvalidSampleTemperature  (1)

If the SampleTemperature cannot be set to the specified value for the provided InjectionVolume, an error is thrown:

TooManyBuffers  (1)

At most four different BufferAs and four different BufferBs may be specified, including Standards/Blanks/ColumnPrime/ColumnFlush (if using Model[Instrument, FPLC, "AKTApurifier UPC 10"], only one each is allowed):

TooManyWavelengths  (1)

If more wavelengths are specified than are supported by the instrument, an error is thrown:

UnsupportedWavelength  (1)

If using Model[Instrument, FPLC, "AKTApurifier UPC 10"], only 280 Nanometer or 254 Nanometer are supported: