Wash
Wash[washingRules]⟹primitive
generates a solid phase synthesis primitive that describes how previous step's chemicals will be removed from resin by flowing solvent through it into waste.
Key | Value Pattern | Description |
| Sample | ObjectP[{Object[Sample,Chemical],Model[Sample,Chemical],Object[Sample,StockSolution],Model[Sample,StockSolution]}] | The solution which will be used to perform the wash. |
| Time | GreaterP[0 Second] | The amount of time for which the wash solution will be mixed with the resin. |
| Volume | GreaterP[0 Microliter] | The volume of wash solution used. |
| NumberOfReplicates | GreaterP[0] | Number of times the wash step will be repeated. |
Input
| washingRules | {Sample -> ObjectP[{Object[Sample, Chemical], Model[Sample, Chemical], Object[Sample, StockSolution], Model[Sample, StockSolution]}], Time -> GreaterP[0*Second], Volume -> GreaterP[0*Microliter], NumberOfReplicates -> GreaterP[0]} | Specific key/value pairs specifying conditions to be used to wash a synthesis vessel. |
Output
| primitive | SynthesisCycleStepP | A solid phase synthesis primitive containing information for executing a wash step during the synthesis. |
Attributes
| Protected |
Create a primitve to wash the resin during the synthesis:
The sample provided to the primitive must have a liquid state:
Sample, Time, Volume and NumberOfReplicates keys are all required:
The Wash Head is protected:
ExperimentPNASynthesis ExperimentDNASynthesis Swell Capping Deprotect Deprotonate Couple ActivateMonomer Cleave
Made for Mathematica By Emerald Cloud Lab | Last modified in April 2018 | Authored By: paul