ExperimentMeasureSurfaceTension

ExperimentMeasureSurfaceTension[Samples]⟹Protocol

generates a Protocol object for determining the surface tensions of input Samples at varying concentrations.

Surface Tension is the energy required to form a new surface by unit area. The surface tension of a sample is determined by measuring the forces exerted on a small diameter rod as it is withdrawn from a sample. Typically, polar solvents have higher surface tensions than their non-polar counterparts and the adsorption of surfactants lowers surface tension. Surfactants also form micelles above critical micelle concentration. Above this concentration, all additional surfactant increases the micelle concentration rather than the concentration of surfactant monomers. The surface tension decreases with increasing surfactant concentration up to this point and then remains constant.

Experimental Principles

Figure 1.1: Procedural overview of a MeasureSurfaceTension experiment. Step 1: Samples are mixed with Diluent to create a SerialDilutionCurve or DilutionCurve. After the samples are diluted, they are loaded onto an assay plate. Step 2: Samples are left to equilibrate. Step 3: The probes are cleaned by dipping them into cleaning solutions and/or by burning off the contaminants with a probe cleaner. Step 4: The probes are then calibrated by measuring a Calibrant with known surface tension. After calibration, the surface tensions of the samples are measured with optional cleaning between measurements.

Instrumentation

Kibron Delta 8 Tensiometer

Figure 2.1.1: An assay plate, probe cleaner and cleaning solutions sit on a moveable table. The table moves left and right to position these objects under probes hanging from microbalances. The table then moves up and down to either dip the probes in and out of the cleaning solutions, put them in contact with a probe cleaner that heats up to 1000C or dip them in and out of a sample. The forces on the probes as they are pulled out of samples is used to calculate their surface tension.

Experiment Options

General

Instrument

The tensiometer that is used to measure the surface tension of the sample.

Default Value: Model[Instrument, Tensiometer, Kibron Delta 8 Tensiometer]

Pattern Description: An object of type or subtype Model[Instrument, Tensiometer] or Object[Instrument, Tensiometer]

Programmatic Pattern: ObjectP[{Model[Instrument, Tensiometer], Object[Instrument, Tensiometer]}]

NumberOfReplicates

The number of times the given dilution series should be replicated to obtain additional surface tension measurements.

Default Value: Null

Pattern Description: Greater than or equal to 2 in increments of 1 or Null.

Programmatic Pattern: GreaterEqualP[2, 1] | Null

PreparedPlate

Indicates if the input plates have been prepared prior to the start of the experiment. If True, the samples in column 12 of the plate will be used as a Calibrant.

Figure 3.1: Use the PreparedPlate option to input an already prepared plate into the experiment. Column 12 of the plate will be used as a calibrant.

Default Value: False

Pattern Description: True or False.

Programmatic Pattern: BooleanP

Protocol

SampleLoadingVolume

The volume of the sample and diluent mixture that is loaded into each well.

Default Value: 50 microliters

Pattern Description: Greater than or equal to 40 microliters and less than or equal to 60 microliters.

Programmatic Pattern: RangeP[40*Microliter, 60*Microliter]

Sample Preparation

DilutionCurve

The collection of dilutions that will be performed on sample before measuring surface tension. SampleLoadingVolume of each dilution will be transferred to the measurement plate. For Fixed Dilution Volume Dilution Curves, the Sample Amount is the volume of the sample that will be mixed the Diluent Volume of the Diluent to create a desired concentration. For Fixed Dilution Factor Dilution Curves, the Sample Volume is the volume of the sample that will created after being diluted by the Dilution Factor. For example, a 1M sample with a dilution factor of 0.7 will be diluted to a concentration 0.7M.

Figure 3.2: Use the DilutionCuve option to create a collection of dilutions that will be performed on the sample before measuring surface tension.

Default Value: Automatic

Default Calculation: This is automatically set Null if Diluent is set to Null or a Serial Dilution Curve is specified.

Pattern Description: Fixed Dilution Factor or Fixed Dilution Volume or Null.

Programmatic Pattern: (({{GreaterEqualP[0*Microliter], GreaterEqualP[0*Microliter]}..} | {{GreaterEqualP[40*Microliter], RangeP[0, 1]}..}) | Automatic) | Null

Index Matches to: experiment samples

SerialDilutionCurve

The collection of dilutions that will be performed on sample before measuring surface tension. SampleLoadingVolume of each dilution will be transferred to the measurement plate. For Serial Dilution Volumes, the Transfer Volume is taken out of the sample and added to a second well with the Diluent Volume of the Diluent. It is mixed, then the Transfer Volume is taken out of that well to be added to a third well. This is repeated to make Number Of Dilutions diluted samples. For example, if a 100mM sample with a Transfer Volume of 20 Microliters, a Diluent Volume of 60 Microliters and a Number of Dilutions of 3 is used, it will create a DilutionCurve of 100mM, 25mM, 6.25mM, 1.5625mM and 0mM with each dilution having a volume of 60 Microliters. For Serial Dilution Factors, the sample will be diluted by the dilution factor at each transfer step.

Figure 3.3: Use the SerialDilutionCuve option to create a collection of serial dilutions that will be performed on the sample before measuring surface tension.

Default Value: Automatic

Default Calculation: This is automatically set Null if Diluent is set to Null or a non-serial Dilution Curve is specified. In all other cases it is automatically set to a Sample Volume of 100 Microliters and a Constant Dilution Factor of 0.7. The Number Of Dilutions is automatically set to 21 if the Diluent is the same as the Calibrant and 20 if they are different.

Pattern Description: Serial Dilution Factor or Serial Dilution Volumes or Null.

Programmatic Pattern: (({GreaterP[0*Microliter], GreaterEqualP[40*Microliter], GreaterP[1, 1]} | {GreaterEqualP[40*Microliter], {RangeP[0, 1], GreaterP[1, 1]} | {RangeP[0, 1]..}}) | Automatic) | Null

Index Matches to: experiment samples

Diluent

The sample that is used to dilute the sample to make a DilutionCurve.

Default Value: Automatic

Default Calculation: If the measurement is to be performed with a DilutionCurve that has No Dilution, this is automatically set to Null. Otherwise, it is automatically set to Model[Sample, "Milli-Q water"].

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

DilutionContainer

The containers in which each sample is diluted with the Diluent to make the concentration series, with indices indicating specific grouping of samples if desired.

Default Value: Automatic

Default Calculation: Automatically set as Model[Container,Plate,"96-well 2mL Deep Well Plate"], grouping samples with the same container and DilutionStorageCondition.

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[{Model[Container]}] | {GreaterEqualP[1, 1] | Automatic, ObjectP[{Model[Container]}] | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

DilutionStorageCondition

The conditions under which any leftover samples from the DilutionCurve should be stored after the samples are transferred to the measurement plate.

Default Value: Automatic

Default Calculation: Automatically set Disposal if the sample is diluted and Null otherwise.

Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.

Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null

Index Matches to: experiment samples

DilutionMixVolume

The volume that is pipetted out and in of the dilution to mix the sample with the Diluent to make the DilutionCurve.

Default Value: Automatic

Default Calculation: Automatically set to the TransferVolume for serial dilutions, and 40 Microliters for fixed dilutions.

Pattern Description: Greater than or equal to 0 microliters and less than or equal to 900 microliters or Null.

Programmatic Pattern: (RangeP[0*Microliter, 900*Microliter] | Automatic) | Null

Index Matches to: experiment samples

DilutionNumberOfMixes

The number of pipette out and in cycles that is used to mix the sample with the Diluent to make the DilutionCurve.

Default Value: Automatic

Default Calculation: Automatically set 5 if the sample is diluted and Null otherwise.

Pattern Description: Greater than or equal to 0 and less than or equal to 20 in increments of 1 or Null.

Programmatic Pattern: (RangeP[0, 20, 1] | Automatic) | Null

Index Matches to: experiment samples

DilutionMixRate

The speed at which the DilutionMixVolume is pipetted out and in of the dilution to mix the sample with the Diluent to make the DilutionCurve.

Default Value: Automatic

Default Calculation: Automatically set to 100 Microliter/Second if the sample is diluted and Null otherwise.

Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 250 microliters per second or Null.

Programmatic Pattern: (RangeP[0.4*(Microliter/Second), 250*(Microliter/Second)] | Automatic) | Null

Index Matches to: experiment samples

ImageDilutionContainers

Indicates if the dilution container should be imaged.

Default Value: False

Pattern Description: True or False.

Programmatic Pattern: BooleanP

SingleSamplePerProbe

Indicates if the samples should be arranged on the measurement plate so that there is only one input sample in each row. This will make sure only one sample will come into contact with each probe. The dilution curves of the samples will arranged so that they are measured with increasing concentration. If the dilution curves of the samples do not take up full rows, some of the wells will remain empty. If this is set to False, the samples will arranged in columns 1-11 of the plate without skipping any wells. Column 12 is taken up by the calibrant. The plate is read right to left, starting with column 12.

Default Value: Automatic

Default Calculation: Automatically set to False if there are no dilution curves, a CleaningMethod is specified and there are more than eight samples. It is set to True in all other cases.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Cleaning

CleaningSolution

The first solution used to clean the tensiometer probes. The probes are dipped into three solutions during the cleaning process.

Default Value: Automatic

Default Calculation: Automatically set to Model[Sample, StockSolution, "70% ethanol cleaning solution for tensiometer"] if the PreCleaningMethod or CleaningMethod involves Solution and Null otherwise.

Pattern Description: An object of type or subtype Model[Sample], Object[Sample], or Object[Container, Vessel] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample], Object[Container, Vessel]}] | _String) | Automatic) | Null

SecondaryCleaningSolution

The second solution used to clean the tensiometer probes. The probes are dipped into three solutions during the cleaning process.

Default Value: Automatic

Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if the PreCleaningMethod or CleaningMethod involves Solution and Null otherwise.

Pattern Description: An object of type or subtype Model[Sample], Object[Sample], or Object[Container, Vessel] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample], Object[Container, Vessel]}] | _String) | Automatic) | Null

TertiaryCleaningSolution

The third solution used to clean the tensiometer probes. The probes are dipped into three solutions during the cleaning process.

Default Value: Null

Pattern Description: An object of type or subtype Model[Sample], Object[Sample], or Object[Container, Vessel] or a prepared sample or Null.

Programmatic Pattern: (ObjectP[{Model[Sample], Object[Sample], Object[Container, Vessel]}] | _String) | Null

PreCleaningMethod

The method used to clean the tensiometer probes before measurements begin. If Burn is selected, the probes will be cleaned with an internal probe cleaner designed to heat off contaminants. This probe cleaning process involves the probes coming into contact with a hot surface for three short preheating pulses, followed by a long burning pulse and a cooling off time. If Solution is selected the probes will be dipped in and out of a cleaning solution. If both are selected, the probes will be cleaned with the solution then burned with the probe cleaner.

Default Value: {Solution, Burn}

Pattern Description: A selection of one or more of Burn or Solution.

Programmatic Pattern: ListableP[Burn | Solution]

CleaningMethod

The method used to clean the tensiometer probes between all measurements within a single dilution curve. The probes are always cleaned before a new set of measurements and the samples in dilution series are measured starting with the most dilute samples. If Burn is selected, the probes will be cleaned with an internal probe cleaner designed to heat off contaminants. This probe cleaning process involves the probes coming into contact with a hot surface for three short preheating pulses, followed by a long burning pulse and a cooling off time. If Solution is selected the probes will be dipped in and out of a cleaning solution. If both are selected, the probes will be cleaned with the solution then burned with the probe cleaner.

Default Value: Null

Pattern Description: A selection of one or more of Burn or Solution or Null.

Programmatic Pattern: ListableP[Burn | Solution] | Null

PreheatingTime

The duration of one of three short heating pulses used by the instrument's internal probe cleaner to preheat the probes before the primary cleaning pulse.

Default Value: 0.8 seconds

Pattern Description: Greater than or equal to 0 seconds and less than or equal to 2 seconds.

Programmatic Pattern: RangeP[0*Second, 2*Second]

BurningTime

The duration of the primary cleaning pulse used by the instrument's internal probe cleaner to burn contaminants off the probes before a new plate is measured.

Default Value: 10 seconds

Pattern Description: Greater than or equal to 0 seconds and less than or equal to 20 seconds.

Programmatic Pattern: RangeP[0*Second, 20*Second]

CoolingTime

The duration of the cooling period after the instrument's internal probe cleaner issues a primary cleaning pulse and before a new plate is measured.

Default Value: 10 seconds

Pattern Description: Greater than or equal to 0 seconds and less than or equal to 20 seconds.

Programmatic Pattern: RangeP[0*Second, 20*Second]

BetweenMeasurementBurningTime

The duration of the primary cleaning pulse used by the instrument's internal probe cleaner to burn contaminants off the probes between all measurements within a single dilution curve (of the same sample).

Default Value: 5 seconds

Pattern Description: Greater than or equal to 0 seconds and less than or equal to 10 seconds or Null.

Programmatic Pattern: RangeP[0*Second, 10*Second] | Null

BetweenMeasurementCoolingTime

The duration of the cooling period between measurements after the instrument's internal probe cleaner issues a primary cleaning pulse and before a new measurement within a single dilution curve (of the same sample).

Default Value: 5 seconds

Pattern Description: Greater than or equal to 0 seconds and less than or equal to 10 seconds or Null.

Programmatic Pattern: RangeP[0*Second, 10*Second] | Null

MaxCleaningNoise

The maximum noise accepted before the probes are brought into contact with the instrument's internal probe cleaner.

Default Value: 0.05 millinewtons per meter

Pattern Description: Greater than or equal to 0.05 millinewtons per meter and less than or equal to 0.2 millinewtons per meter.

Programmatic Pattern: RangeP[0.05*Milli*(Newton/Meter), 0.2*Milli*(Newton/Meter)]

Post Experiment

SamplesOutStorageCondition

The conditions under which the samples in the assay plate should be stored after the protocol is completed. The samples in the plate will be transferred into ContainerOut.

Default Value: Disposal

Programmatic Pattern: SampleStorageTypeP | Disposal

Index Matches to: experiment samples

ContainerOut

The container in which the samples in the assay plate are transferred into after the experiment.

Default Value: Automatic

Default Calculation: Automatically set to Null if the SamplesOutStorageCondition is set to Disposal and Model[Container,Plate,"96-well 2mL Deep Well Plate"] in all other cases.

Pattern Description: An object of type or subtype Model[Container] or Null.

Programmatic Pattern: (ObjectP[Model[Container]] | Automatic) | Null

SamplesInStorageCondition

The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.

Default Value: Null

Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null

Index Matches to: experiment samples

Calibration

NumberOfCalibrationMeasurements

The number of times surface tension of the calibration liquid is measured before taking the sample measurements. The average of these measurements is used in the calibration.

Default Value: 3

Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1.

Programmatic Pattern: RangeP[1, 20, 1]

Calibrant

The sample that is used to measure a calibration factor used to convert the readings from the balances to surface tension values. Each probe is calibrated before the surface tensions of a sample's dilution curve is measured. A sample with a known surface tension is needed for this calibration.

Default Value: Automatic

Default Calculation: Automatically set to the Diluent if the Diluent has a populated SurfaceTension field or Model[Sample, "Milli-Q water"] in all other cases.

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.

Programmatic Pattern: (ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic

CalibrantSurfaceTension

The surface tension value of the calibrant that is used to perform the calibration.

Default Value: Automatic

Default Calculation: Automatically set to be the SurfaceTension value stored in the model of the selected Calibrant.

Pattern Description: Greater than or equal to 10 millinewtons per meter and less than or equal to 100 millinewtons per meter.

Programmatic Pattern: RangeP[10*Milli*(Newton/Meter), 100*Milli*(Newton/Meter)] | Automatic

MaxCalibrationNoise

The maximum noise (probe vibration) accepted while the probes are in the air before the probes are moved into the calibration sample.

Default Value: 0.05 millinewtons per meter

Pattern Description: Greater than or equal to 0.05 millinewtons per meter and less than or equal to 0.2 millinewtons per meter.

Programmatic Pattern: RangeP[0.05*Milli*(Newton/Meter), 0.2*Milli*(Newton/Meter)]

Measurement

EquilibrationTime

The minimum amount of time, after being plated but before readings begin, used for the sample to equilibrate.

Default Value: 5 minutes

Pattern Description: Greater than or equal to 0 minutes and less than or equal to 120 minutes.

Programmatic Pattern: RangeP[0*Minute, 120*Minute]

NumberOfSampleMeasurements

The number of times the surface tension of each dilution of the sample is measured. These measurements are taken consecutively before moving to the next column of the plate.

Default Value: 1

Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1.

Programmatic Pattern: RangeP[1, 20, 1]

ProbeSpeed

The percentage of the default speed that is used to move the measurement table down, pulling the probe out of the liquid. Smaller settings improve accuracy of samples with higher viscosities. A probe speed of 100% is appropriate for samples with viscosities in the 0-40 cP range. A probe speed of 30% can measure samples with viscosities up to 100 cP.

Default Value: 100. percent

Pattern Description: Greater than or equal to 30 percent and less than or equal to 100 percent.

Programmatic Pattern: RangeP[30*Percent, 100*Percent]

MaxDryNoise

The maximum noise (probe vibration) accepted while the probes are in the air before the probes are moved into the sample.

Default Value: 0.2 millinewtons per meter

Pattern Description: Greater than or equal to 0.05 millinewtons per meter and less than or equal to 0.2 millinewtons per meter.

Programmatic Pattern: RangeP[0.05*Milli*(Newton/Meter), 0.2*Milli*(Newton/Meter)]

MaxWetNoise

The maximum noise (probe vibration) accepted while the probes are in the sample liquid before the probes are pulled out of the liquid to take the measurement.

Default Value: 0.05 millinewtons per meter

Pattern Description: Greater than or equal to 0.05 millinewtons per meter and less than or equal to 0.2 millinewtons per meter.

Programmatic Pattern: RangeP[0.05*Milli*(Newton/Meter), 0.2*Milli*(Newton/Meter)]

Sample Prep Options

Sample Preparation

PreparatoryUnitOperations

Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSampleManipulation.

Default Value: Null

Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.

Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null

PreparatoryPrimitives

Default Value: Null

Pattern Description: List of one or more a primitive with head Define, Transfer, Mix, Aliquot, Consolidation, FillToVolume, Incubate, Filter, Wait, Centrifuge, or Resuspend entries or Null.

Programmatic Pattern: {SampleManipulationP..} | Null

Preparatory Incubation

Incubate

Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

IncubationTemperature

Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.

Default Value: Automatic

Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.

Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null

Index Matches to: experiment samples

IncubationTime

Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

Mix

Indicates if this sample should be mixed while incubated, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

MixType

Indicates the style of motion used to mix the sample, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on the container of the sample and the Mix option.

Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.

Programmatic Pattern: (MixTypeP | Automatic) | Null

Index Matches to: experiment samples

MixUntilDissolved

Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

MaxIncubationTime

Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.

Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

IncubationInstrument

The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.

Default Value: Automatic

Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.

Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.

Programmatic Pattern: (ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]] | Automatic) | Null

Index Matches to: experiment samples

AnnealingTime

Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.

Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquotContainer

The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquotDestinationWell

The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

IncubateAliquot

The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

Preparatory Centrifugation

Centrifuge

Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

CentrifugeInstrument

The centrifuge that will be used to spin the provided samples prior to starting the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null

Index Matches to: experiment samples

CentrifugeIntensity

The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.

Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeTime

The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.

Default Value: Automatic

Pattern Description: Greater than 0 minutes or Null.

Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null

Index Matches to: experiment samples

CentrifugeTemperature

The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.

Default Value: Automatic

Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.

Programmatic Pattern: ((Ambient | RangeP[-10*Celsius, 40*Celsius]) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquotContainer

The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquotDestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

CentrifugeAliquot

The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

Preparatory Filtering

Filtration

Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).

Default Value: Automatic

Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

FiltrationType

The type of filtration method that should be used to perform the filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.

Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.

Programmatic Pattern: (FiltrationTypeP | Automatic) | Null

Index Matches to: experiment samples

FilterInstrument

The instrument that should be used to perform the filtration.

Default Value: Automatic

Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.

Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null

Index Matches to: experiment samples

Filter

The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.

Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.

Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null

Index Matches to: experiment samples

FilterMaterial

The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.

Pattern Description: Cellulose, Cotton, Polyethylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.

Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null

Index Matches to: experiment samples

PrefilterMaterial

The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.

Pattern Description: Cellulose, Cotton, Polyethylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.

Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null

Index Matches to: experiment samples

FilterPoreSize

The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.

Default Value: Automatic

Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.

Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.

Programmatic Pattern: (FilterSizeP | Automatic) | Null

Index Matches to: experiment samples

PrefilterPoreSize

The pore size of the filter; all particles larger than this should be removed during the filtration.

Default Value: Automatic

Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.

Programmatic Pattern: (FilterSizeP | Automatic) | Null

Index Matches to: experiment samples

FilterSyringe

The syringe used to force that sample through a filter.

Default Value: Automatic

Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.

Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

FilterHousing

The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.

Default Value: Automatic

Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.

Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.

Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null

Index Matches to: experiment samples

FilterIntensity

The rotational speed or force at which the samples will be centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.

Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null

Index Matches to: experiment samples

FilterTime

The amount of time for which the samples will be centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than 0 minutes or Null.

Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null

Index Matches to: experiment samples

FilterTemperature

The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.

Default Value: Automatic

Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.

Pattern Description: Greater than or equal to 4 degrees Celsius or Null.

Programmatic Pattern: ((Alternatives[GreaterEqualP[4*Celsius]]) | Automatic) | Null

Index Matches to: experiment samples

FilterContainerOut

The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.

Default Value: Automatic

Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.

Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.

Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

FilterAliquotDestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or Null.

Programmatic Pattern: (WellPositionP | Automatic) | Null

Index Matches to: experiment samples

FilterAliquotContainer

The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.

Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null

Index Matches to: experiment samples

FilterAliquot

The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.

Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null

Index Matches to: experiment samples

FilterSterile

Indicates if the filtration of the samples should be done in a sterile environment.

Default Value: Automatic

Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Index Matches to: experiment samples

Aliquoting

Aliquot

Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).

Default Value: Automatic

Pattern Description: True or False.

Programmatic Pattern: BooleanP | Automatic

Index Matches to: experiment samples

AliquotAmount

The amount of a sample that should be transferred from the input samples into aliquots.

Default Value: Automatic

Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.

Pattern Description: All or Count or Count or Mass or Volume or Null.

Index Matches to: experiment samples

TargetConcentration

The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.

Default Value: Automatic

Default Calculation: Automatically calculated based on aliquot and buffer volumes.

Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.

Programmatic Pattern: ((GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)]) | Automatic) | Null

Index Matches to: experiment samples

TargetConcentrationAnalyte

The substance whose final concentration is attained with the TargetConcentration option.

Default Value: Automatic

Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.

Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.

Programmatic Pattern: (ObjectP[IdentityModelTypes] | Automatic) | Null

Index Matches to: experiment samples

AssayVolume

The desired total volume of the aliquoted sample plus dilution buffer.

Default Value: Automatic

Default Calculation: Automatically determined based on Volume and TargetConcentration option values.

Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.

Programmatic Pattern: (RangeP[1*Microliter, 20*Liter] | Automatic) | Null

Index Matches to: experiment samples

ConcentratedBuffer

The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

BufferDilutionFactor

The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentrationFactor of that sample; otherwise, set to Null.

Pattern Description: Greater than or equal to 1 or Null.

Programmatic Pattern: (GreaterEqualP[1] | Automatic) | Null

Index Matches to: experiment samples

BufferDiluent

The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

AssayBuffer

The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.

Default Value: Automatic

Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.

Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.

Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null

Index Matches to: experiment samples

AliquotSampleStorageCondition

The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.

Default Value: Automatic

Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null

Index Matches to: experiment samples

DestinationWell

The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.

Default Value: Automatic

Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.

Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.

Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

AliquotContainer

The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.

Default Value: Automatic

Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.

Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries or Null.

Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

AliquotPreparation

Indicates the desired scale at which liquid handling used to generate aliquots will occur.

Default Value: Automatic

Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.

Pattern Description: Manual or Robotic or Null.

Programmatic Pattern: (PreparationMethodP | Automatic) | Null

ConsolidateAliquots

Indicates if identical aliquots should be prepared in the same container/position.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Protocol Options

Organizational Information

Template

A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.

Default Value: Null

Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.

Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

Name

A object name which should be used to refer to the output object in lieu of an automatically generated ID number.

Default Value: Null

Pattern Description: A string or Null.

Programmatic Pattern: _String | Null

Post Experiment

MeasureWeight

Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

MeasureVolume

Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

ImageSample

Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.

Default Value: Automatic

Pattern Description: True or False or Null.

Programmatic Pattern: (BooleanP | Automatic) | Null

Example Calls

Sample Dilution

Generate a serial dilution curve to dilute the sample. Provide the volume of the sample, a dilution factor and the number of times the sample should be diluted. This should be used if you want the sample to be diluted by the same factor at each step of the serial dilution. The volume of the sample will be the final volume of the sample after the dilution is performed. The example call below will create a series of samples with dilution factors of (1, 0.7, 0.49, 0.34, 0.24, 0.17, 0.12, 0) and a volume of 60 Microliters.

Generate a serial dilution curve to dilute the sample. Provide the volume of the sample and a series of dilution factors the sample should be diluted by at each step. This should be used if you want the sample to be diluted by different factors at each step of the serial dilution. The volume of the sample will be the final volume of the sample after the dilution is performed. The example call below will create a series of samples with dilution factors of (1, 0.7, 0.49, 0.34, 0.31, 0.28, 0.25, 0) and a volume of 60 Microliters.

Generate a serial dilution curve to dilute the sample. Provide the volume of the sample that should be transferred, the volume of diluent the sample should be added to and the number of times the sample should be diluted. This should be used if you want give the volumes the sample will be diluted by with instead of dilution factors. The example call below will create a series of samples with dilution factors of (1, 0.67, 0.44, 0.30, 0.20, 0.13, 0.09, 0) and a volume of 50 Microliters.

Generate a dilution curve to dilute the sample. Provide the volume of the sample and dilution factor at each point of the curve:

Provide samples that do not need any dilution:

Sample Storage

Save any extra volume created by the dilution by setting the DilutionStorageCondition:

Preferred Input Containers

The liquid handlers can dilute from samples in 2mL and 50 mL tubes as well as 1ml and 2ml Deep Well Plates. The assay is performed in a DynePlate 96-well plate.

Warnings and Errors

Messages (34)

CalibrantSurfaceTensionMismatch (1)

Warn that a dilution was given even though the dilution mix rate was set to null:

CannotSpecifyBothDilutionCurveAndSerialDilutionCurve (1)

If both a serial dilution curve and a custom dilution curve is given, an error will be thrown:

ConflictingCleaningSolution (1)

If a cleaning solution is given and cleaning method solution is not given, a warning will be thrown:

ConflictingDilutionContainer (1)

If multiple dilution containers are given with the same index, an error will be thrown:

ConflictingDilutionMixSettings (1)

If a samples with conflicting dilution mix settings are given, an error will by thrown:

ConflictingDilutionMixVolume (1)

Warn that there no dilution was given even though there was a dilution mix volume specified:

ConflictingPreparedPlateCalibrants (1)

Warn that the calibrant samples in PreparedPlate do not have the same composition:

ConflictingPreparedPlateDilution (1)

If a prepared plate is given and dilution options are not Null, an error will by thrown:

ContainerOutMismatch (1)

If a non disposal storage condition is given with no containers out, a error will by thrown:

DilutionContainerConflictingStorage (1)

If any the dilution container has samples that have different storage conditions, an error will be thrown:

DilutionContainerInsufficientVolume (1)

If the dilution container is too small, an error will be thrown:

DilutionCurveExcessVolume (2)

Warn that there will be excess sample from a serial dilution curve:

DiscardedSamples (1)

If the provided sample is discarded, an error will be thrown:

IncompatableDilutionContainer (1)

If the sample manipulation cannot be done on the liquid handler because of the containers, throw an error:

InsufficientDilutionCurveVolume (1)

If any sample in the dilution curve has a volume below 50ul, an error will be thrown:

InvalidAssayPlate (1)

If a prepared plate is given and the samples are not in an appropriate assay plate, an error will by thrown:

MeasureSurfaceTesnsionContainerOutConflictingStorage (1)

If the samples out storage condition varies between samples, an error will be thrown:

MissingCalibrantSurfaceTension (1)

If a calibrant is givin without a known surface tension is given, an error will be thrown:

MissingCleaningMethod (2)

Warn that there will be no cleaning between measurements, even though multiple samples are measured per probe:

MissingDilutionContainer (1)

If a dilution curve is given with no dilution container, an error will be thrown:

MissingDilutionCurve (2)

Warn that there will be no dilution even though a diluent was given:

MissingDilutionMixRate (1)

Warn that a dilution was given even though the dilution mix rate was set to null:

MissingDilutionMixVolume (1)

Warn that there no dilution mix volume was given even though there is diluting:

MissingDilutionNumberOfMixes (1)

Warn that a dilution was given even though the dilution number of mixes was set to null:

MultipleAssayPlates (1)

If a samples that take up more than one assay plate are given, a warning will by thrown:

MustSpecifyCleaningSolution (1)

If a cleaning method with solution is given and the cleaning solution is null, an error will be thrown:

MustSpecifyDiluent (1)

If a dilution curve is given with no diluent, an error will be thrown:

NoCalibrantProvided (1)

If a prepared plate is given and the samples and there is no calibrant in the plate, an error will by thrown:

PreparedPlateFalse (2)

Warn that the samples are in an assay plate but PreparedPlate is False:

SamplesOutStorageConditionMismatch (1)

If a containers out is given with no storage condition, a warning will by thrown:

Possible Issues

Evaporation

During long equilibration times, evaporation can cause the decrease in size to a point where the probes cannot touch the sample. This will result in zero surface tension values.

Last modified on Tue 10 Jan 2023 14:57:12