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ExperimentRoboticSamplePreparation

ExperimentRoboticSamplePreparation[UnitOperations]⟹Protocol

creates a Protocol object that will perform the robotic sample preparation specified in UnitOperations.

    
ExperimentRoboticSamplePreparation allows for the incubation, mixing, centrifugation, filtration, pelleting, and other general preparation of samples to be performed in-line with transfers of liquids (via LabelSample, LabelContainer, and Transfer) on a robotically integrated liquid handler. To prepare samples both manually and robotically, please consult ExperimentSamplePreparation.
    

Experimental Principles

    Figure 1.1: Sample Preparation unit operations are performed manually or robotically on solid or liquid samples in the user specified manner.
    Figure 1.2: The following unit operations can be used robotically.

Instrumentation

    Hamilton STARlet

    Figure 2.1.1: An overview of the robotic workcell. It is capable of processing plate operations including incubation, shaking, and magnetic bead separation on-deck and filtration and absorbance/fluorescence/luminescence measurement off-deck, with a robot arm facilitating the plate movements.
    Figure 2.1.2: Overhead view of the liquid handling instrument deck.

    Super STAR

    Figure 2.2.1: An overview of the robotic workcell. It is capable of processing plate operations including incubation, shaking, and magnetic bead separation on-deck and centrifugation, filtration, and absorbance/fluorescence/luminescence measurement off-deck, with a robot arm facilitating the plate movements.
    Figure 2.2.2: Overhead view of the liquid handling instrument deck.

LabelSample

    Options

    The options that specify the model, amount, and container of this sample.
    Pattern: {_Rule..}

    General

    Label

    The label of the samples that are to be prepared.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String
    Index Matches to: Label

    Restricted

    Indicates whether the sample should be restricted from automatic use is any of your team's experiments that request the sample's models. (Restricted samples can only be used in experiments when specifically provided as input to the experiment functions by a team member). Setting the option to Null means the sample should be untouched. Setting the option to True or False will set the Restricted field of the sample to that value respectively.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Sample

    The sample that should be labeled by this primitive.
    Default Value: Automatic
    Default Calculation: Automatically set from the Container, if that option is specified.
    Pattern Description: Container with Well Position or Sample Model or Sample Object.
    Programmatic Pattern: (ObjectP[{Model[Sample]}] | (ObjectP[{Object[Sample]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Automatic
    Index Matches to: Label

    Container

    The container object of the sample that is to be labeled.
    Default Value: Automatic
    Default Calculation: Automatically resolves to an Object[Container] if an Object[Sample] is specified. Otherwise, automatically resolves to a Model[Container] on any existing samples that can be used to fulfill the Model[Sample] request or based on the container that the default product for the Model[Sample] comes in.
    Pattern Description: Existing Container or Single Container Model.
    Programmatic Pattern: ((ObjectP[Object[Container]] | _String) | ObjectP[Model[Container]]) | Automatic
    Index Matches to: Label

    Well

    The well of the container that the Object[Sample] is already in.
    Default Value: Automatic
    Default Calculation: Automatically resolves from the Sample if it is an Object[Sample].
    Pattern Description: A string that matches the pattern: WellPositionP.
    Programmatic Pattern: WellPositionP | Automatic
    Index Matches to: Label

    ContainerLabel

    The label to assign to the container that is used for the sample.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: (Null | _String) | Null
    Index Matches to: Label

    Amount

    The requested amount of the Model[Sample] to put in the specified Container.
    Default Value: Automatic
    Default Calculation: Automatically set based on the usage of the sample label in other primitives.
    Pattern Description: Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Milligram] | GreaterP[0*Milliliter] | GreaterEqualP[1, 1])) | Automatic) | Null
    Index Matches to: Label

    ExactAmount

    Indicates that an Object[Sample] with the exact Amount specified (+/- Tolerance) should be picked in the lab. If set to False, existing samples with an amount greater than or equal to the amount requested can be used. ExactAmount should be set to True if you are depending on the sample having an exact volume/mass/count in the lab. However, if you're just transferring out of this sample and don't care about its exact volume, setting ExactAmount->False will save time in the lab (the exact amount doesn't need to first be aliquotted out).
    Default Value: Automatic
    Default Calculation: Automatically set to True if the sample has FixedAmounts or if the user has manually specified the Amount option.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Label

    Tolerance

    The allowed tolerance when preparing the specified Amount of sample. This option can only be set if ExactAmount is set to True.
    Default Value: Automatic
    Default Calculation: Automatically set to 1% of the requested Amount if ExactAmount is set to True.
    Pattern Description: Count or Mass or Percent Tolerance or Volume or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Milligram] | GreaterP[0*Milliliter] | GreaterEqualP[1, 1] | RangeP[0*Percent, 100*Percent])) | Automatic) | Null
    Index Matches to: Label

    Density

    Indicates the known density of the sample being labeled at room temperature. By setting upfront, this allows ECL to skip measuring the density later.
    Default Value: Null
    Pattern Description: Greater than 0 grams per milliliter or Null.
    Programmatic Pattern: (Null | GreaterP[(0*Gram)/Milliliter]) | Null
    Index Matches to: Label

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Health & Safety

    SampleModel

    Specifies the model of the given sample. This option should only be used if you want to override the default Model[Sample] of your labeled sample.
    Default Value: Null
    Pattern Description: An object of type or subtype Model[Sample] or Null.
    Programmatic Pattern: (Null | ObjectP[Model[Sample]]) | Null
    Index Matches to: Label

    Composition

    Specifies molecular composition of this sample. This option should only be used if you want to override the automatically calculated Composition of your labeled sample.
    Default Value: Null
    Pattern Description: List of one or more {Amount, Identity Model} entries or Null.
    Programmatic Pattern: (Null | {{(GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)] | RangeP[0*VolumePercent, 100*VolumePercent] | RangeP[0*MassPercent, 100*MassPercent] | RangeP[0*PercentConfluency, 100*PercentConfluency] | GreaterP[0*(Cell/Liter)] | GreaterP[0*(CFU/Liter)] | GreaterP[0*OD600] | GreaterP[0*Colony]) | Null, ObjectP[List @@ IdentityModelTypeP] | Null}..}) | Null
    Index Matches to: Label

    Acid

    Indicates if pure samples of this molecule are strong acids (pH <= 2) and require dedicated secondary containment during storage.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Anhydrous

    Indicates if this sample does not contain water.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    AutoclaveUnsafe

    Indicates if this sample cannot be safely autoclaved.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Base

    Indicates if pure samples of this molecule are strong bases (pH >= 12) and require dedicated secondary containment during storage.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    BiosafetyHandling

    Indicates if this sample must be handled in a biosafety cabinet.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    BiosafetyLevel

    The Biosafety classification of the substance.
    Default Value: Null
    Pattern Description: BSL-1, BSL-2, BSL-3, or BSL-4 or Null.
    Programmatic Pattern: (Null | BiosafetyLevelP) | Null
    Index Matches to: Label

    CellType

    The primary types of cells that are contained within this sample.
    Default Value: Null
    Pattern Description: Mammalian, Plant, Insect, Fungal, Yeast, or Bacterial or Null.
    Programmatic Pattern: (Null | CellTypeP) | Null
    Index Matches to: Label

    CultureAdhesion

    The type of cell culture that is currently being performed to grow these cells.
    Default Value: Null
    Pattern Description: Adherent, Suspension, or SolidMedia or Null.
    Programmatic Pattern: (Null | CultureAdhesionP) | Null
    Index Matches to: Label

    DOTHazardClass

    The Department of Transportation hazard classification of the substance.
    Default Value: Null
    Pattern Description: Class 0, Class 1 Division 1.1 Mass Explosion Hazard, Class 1 Division 1.2 Projection Hazard, Class 1 Division 1.3 Fire, Blast, or Projection Hazard, Class 1 Division 1.4 Limited Explosion, Class 1 Division 1.5 Insensitive Mass Explosion Hazard, Class 1 Division 1.6 Insensitive No Mass Explosion Hazard, Class 2 Division 2.1 Flammable Gas Hazard, Class 2 Division 2.2 Non-Flammable Gas Hazard, Class 2 Division 2.3 Toxic Gas Hazard, Class 3 Flammable Liquids Hazard, Class 4 Division 4.1 Flammable Solid Hazard, Class 4 Division 4.2 Spontaneously Combustible Hazard, Class 4 Division 4.3 Dangerous when Wet Hazard, Class 5 Division 5.1 Oxidizers Hazard, Class 5 Division 5.2 Organic Peroxides Hazard, Class 6 Division 6.1 Toxic Substances Hazard, Class 6 Division 6.2 Infectious Substances Hazard, Class 7 Division 7 Radioactive Material Hazard, Class 8 Division 8 Corrosives Hazard, or Class 9 Miscellaneous Dangerous Goods Hazard or Null.
    Programmatic Pattern: (Null | DOTHazardClassP) | Null
    Index Matches to: Label

    DrainDisposal

    Indicates if pure samples of this molecule may be safely disposed down a standard drain.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    ExpirationDate

    Date after which this sample is considered expired and users will be warned about using it in protocols.
    Default Value: Null
    Pattern Description: A valid date or Null.
    Programmatic Pattern: (Null | _?DateObjectQ) | Null
    Index Matches to: Label

    ExpirationHazard

    Indicates if pure samples of this molecule become hazardous once they are expired and must be automatically disposed of when they pass their expiration date.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Flammable

    Indicates if pure samples of this molecule are easily set aflame under standard conditions.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Fuming

    Indicates if pure samples of this molecule emit fumes spontaneously when exposed to air.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    HazardousBan

    Indicates if pure samples of this molecule are currently banned from usage in the ECL because the facility isn't yet equiped to handle them.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    InertHandling

    Indicates if this sample must be handled in a glove box.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    MSDSFile

    URL of the MSDS (Materials Saftey Data Sheet) PDF file.
    Default Value: Null
    Pattern Description: A cloud file stored on Constellation that ends in .PDF or a string that matches the pattern: URLP or Null.
    Programmatic Pattern: (Null | (URLP | ObjectP[Object[EmeraldCloudFile]])) | Null
    Index Matches to: Label

    MSDSRequired

    Indicates if an MSDS is applicable for this model.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    NFPA

    The National Fire Protection Association (NFPA) 704 hazard diamond classification for the substance.
    Default Value: Null
    Pattern Description: {Health, Flammability, Reactivity, Special} or Null.
    Programmatic Pattern: (Null | {0 | 1 | 2 | 3 | 4, 0 | 1 | 2 | 3 | 4, 0 | 1 | 2 | 3 | 4, DuplicateFreeListableP[Oxidizer | WaterReactive | Aspyxiant | Corrosive | Acid | Bio | Poisonous | Radioactive | Cryogenic | Null] | {}}) | Null
    Index Matches to: Label

    NucleicAcidFree

    Indicates if this sample is presently considered to be not contaminated with DNA and RNA.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    ParticularlyHazardousSubstance

    Indicates if exposure to this substance has the potential to cause serious and lasting harm. A substance is considered particularly harmful if it is categorized by any of the following GHS classifications (as found on a MSDS): Reproductive Toxicity (H340, H360, H362), Acute Toxicity (H300, H310, H330, H370, H373), Carcinogenicity (H350).
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Pungent

    Indicates if pure samples of this molecule must be handled in a ventilated enclosures.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    PyrogenFree

    Indicates if this sample is presently considered to be not contaminated with endotoxin.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Pyrophoric

    Indicates if pure samples of this molecule can ignite spontaneously upon exposure to air.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Radioactive

    Indicates if pure samples of this molecule emit substantial ionizing radiation.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    RNaseFree

    Indicates that this sample is free of any RNases.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    SampleHandling

    The method by which this sample should be manipulated in the lab when transfers out of the sample are requested.
    Default Value: Null
    Pattern Description: Liquid, Slurry, Powder, Itemized, Viscous, Paste, Brittle, Fabric, or Fixed or Null.
    Programmatic Pattern: (Null | SampleHandlingP) | Null
    Index Matches to: Label

    State

    The physical state of the sample when well solvated at room temperature and pressure.
    Default Value: Null
    Pattern Description: Solid, Liquid, or Gas or Null.
    Programmatic Pattern: (Null | (Solid | Liquid | Gas)) | Null
    Index Matches to: Label

    Sterile

    Indicates that this model of sample arrives sterile from the manufacturer.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Ventilated

    Indicates if pure samples of this molecule must be handled in a ventilated enclosures.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    WaterReactive

    Indicates if pure samples of this molecule react spontaneously upon exposure to water.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Storage Information

    StorageCondition

    The condition in which this sample gets stored in when not used by an experiment.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or an object of type or subtype Model[StorageCondition] or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Label

    AsepticTransportContainerType

    Indicates how this sample is contained in an aseptic barrier and if it needs to be unbagged before being used in a protocol, maintenance, or qualification.
    Default Value: Null
    Pattern Description: Individual or Bulk or Null.
    Programmatic Pattern: (Null | AsepticTransportContainerTypeP) | Null
    Index Matches to: Label

    Expires

    Indicates if samples of this model expire after a given amount of time.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    LightSensitive

    Determines if the sample reacts or degrades in the presence of light and should be stored in the dark to avoid exposure.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    ShelfLife

    The length of time after DateCreated that samples of this model are recommended for use before they should be discarded.
    Default Value: Null
    Pattern Description: Greater than 0 days or Null.
    Programmatic Pattern: (Null | GreaterP[0*Day]) | Null
    Index Matches to: Label

    TransferTemperature

    The temperature that this sample should be at before any transfers using this sample occur.
    Default Value: Null
    Pattern Description: Greater than or equal to 4 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: (Null | RangeP[4*Celsius, 90*Celsius]) | Null
    Index Matches to: Label

    TransportTemperature

    The temperature that this sample should be heated or refrigerated while transported between instruments during experimentation.
    Default Value: Null
    Pattern Description: Greater than or equal to -86 degrees Celsius and less than or equal to 10 degrees Celsius or greater than or equal to 30 degrees Celsius and less than or equal to 105 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[-86*Celsius, 10*Celsius] | RangeP[30*Celsius, 105*Celsius])) | Null
    Index Matches to: Label

    UnsealedShelfLife

    The length of time after DateUnsealed that samples of this model are recommended for use before they should be discarded.
    Default Value: Null
    Pattern Description: Greater than 0 days or Null.
    Programmatic Pattern: (Null | GreaterP[0*Day]) | Null
    Index Matches to: Label

    Compatibility

    GloveBoxBlowerIncompatible

    Indicates that the glove box blower must be turned off to prevent damage to the catalyst in the glove box that is used to remove traces of water and oxygen, when manipulating this sample inside of the glove box.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    GloveBoxIncompatible

    Indicates if this sample cannot be used inside of the glove box due high volatility and/or detrimental reactivity with the catalyst in the glove box that is used to remove traces of water and oxygen. Sulfur and sulfur compounds (such as H2S, RSH, COS, SO2, SO3), halides, halogen (Freon), alcohols, hydrazine, phosphene, arsine, arsenate, mercury, and saturation with water may deactivate the catalyst.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    IncompatibleMaterials

    A list of materials that would be damaged if wetted by this model.
    Default Value: Null
    Pattern Description: List of one or more None, ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton, Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc, Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB, Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, VacuumMeltedStainlessSteel, or Wood entries or Null.
    Programmatic Pattern: (Null | {(None | ABS | PLA | Acrylic | AmorphousFluoropolymer | CPVC | CTFE | Cycloolefine | COC | Delrin | ECTFE | EPDM | ETFE | EVA | FEP | FFKM | HDPE | Hypalon | LDPE | NaturalRubber | NBR | Neoprene | Nitrile | Noryl | Nylon | PEEK | PEI | Perlast | PharmaPure | Polycarbonate | Polyester | Polyethylene | Polyisoprene | Polyolefin | Polyoxymethylene | Polypropylene | Polystyrene | Polyurethane | PVC | PCTFE | PETG | PF | PFA | PPS | PTFE | PVDF | SEBS | Silicone | SyntheticRubber | TFM | TPE | Tygon | UVPlastic | UVXPO | Viton | Aluminum | Alloy | AnodisedAluminum | Brass | Bronze | CarbonSteel | CastIron | Chrome | Copper | Elgiloy | Gold | Hastelloy | Lead | Magnesium | Molybdenum | Nickel | Niobium | Platinum | Silver | Steel | StainlessSteel | Titanium | Tungsten | Zinc | Cellulose | Cotton | Polyethylene | Polypropylene | PTFE | Nylon | PES | PLUS | PVDF | GlassFiber | GHP | UHMWPE | EPDM | DuraporePVDF | GxF | ZebaDesaltingResin | NickelResin | Silica | HLB | Silica | Alumina | ResinParticlesWithLatexMicroBeads | CrossLinkedDextranBeads | CrossLinkedPolystyrene | AerisCoreShell | KinetexCoreShell | CrossLinkedAgarose | Vydac218MS | JordiGel | Styrene | SilicaCompositeTWIN | BEH | CSH | HSS | CarboPacPA1 | CarboPacPA10 | BEH | Polysulfone | Agate | AluminiumOxide | ZirconiumOxide | Cardboard | Ceramic | Epoxy | EpoxyResin | BorosilicateGlass | Glass | GlassyCarbon | Graphite | OpticalGlass | Porcelain | Quartz | UVQuartz | ESQuartz | FusedQuartz | IRQuartz | Oxidizer | Ruby | Sapphire | Silicon | Silver | Viton | Styrofoam | VacuumMeltedStainlessSteel | Wood)..}) | Null
    Index Matches to: Label

    LiquidHandlerIncompatible

    Indicates if pure samples of this molecule cannot be reliably aspirated or dispensed on an automated liquid handling robot.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    PipettingMethod

    The pipetting parameters used to manipulate pure samples of this model.
    Default Value: Null
    Pattern Description: An object of type or subtype Model[Method, Pipetting] or Null.
    Programmatic Pattern: (Null | ObjectP[Model[Method, Pipetting]]) | Null
    Index Matches to: Label

    UltrasonicIncompatible

    Indicates if pure samples of this molecule cannot be performed via the ultrasonic distance method due to vapors interfering with the reading.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

LabelContainer

    Options

    The options that specify the model of this container.
    Pattern: {_Rule..}

    General

    Label

    The label of the samples that are to be prepared.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String
    Index Matches to: Label

    Restricted

    Indicates whether the container should be restricted from automatic use is any of your team's experiments that request the container's models. (Restricted can only be used in experiments when specifically provided as input to the experiment functions by a team member). Setting the option to Null means the sample should be untouched. Setting the option to True or False will set the Restricted field of the sample to that value respectively.
    Default Value: Null
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Label

    Container

    The container object that will be labeled for future use.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Container] or Model[Container] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Container], Model[Container]}] | _String)) | Null
    Index Matches to: Label

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

Wait

    duration

    The amount of time to pause during execution of primitives.
    Pattern: GreaterP[0*Second]

    General

    Duration

    The amount of time to pause before continuing the execution of future unit operations.
    Default Value: Null
    Pattern Description: Greater than 0 seconds or Null.
    Programmatic Pattern: GreaterP[0*Second]

Transfer

    transferRules

    The list of key/value pairs specifying the samples, amounts, and destination involved in the transfer primitive.
    Pattern: {Source -> NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}, Amount -> GreaterEqualP[0*Milliliter] | GreaterEqualP[0*Gram] | GreaterEqualP[0, 1], Destination -> NonSelfContainedSampleP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}}

    General

    Source

    The samples or locations from which liquid or solid is transferred.
    Default Value: Null
    Pattern Description: Existing Container with Well Position or New Container with Index or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container], Model[Sample]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Source

    Destination

    The sample or location to which the liquids/solids are transferred.
    Default Value: Null
    Pattern Description: Existing Container with Well Position or New Container with Index or Sample or Container or Waste or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Item], Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String} | Waste
    Index Matches to: Source

    Amount

    The volumes of the samples to be transferred.
    Default Value: Null
    Pattern Description: All or Count or Mass or Volume or Null.
    Programmatic Pattern: RangeP[0.1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0., 1.] | All
    Index Matches to: Source

    SourceLabel

    A user defined word or phrase used to identify the source sample from which the transfer occurs, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    SourceContainerLabel

    A user defined word or phrase used to identify the container of the source sample from which the transfer occurs, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    DestinationLabel

    A user defined word or phrase used to identify the destination sample from which the source sample is transferred into, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    DestinationContainerLabel

    A user defined word or phrase used to identify the container of the destination sample from which the source sample is transferred into, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    SourceWell

    The position in the source container to aspirate from.
    Default Value: Automatic
    Default Calculation: Automatically set to the first non-empty well if an Object[Container] or {_Integer, Model[Container]} is given. Otherwise, is set to the position of the given Object[Sample].
    Pattern Description: Any valid container position or any well from A1 to H12.
    Programmatic Pattern: (WellPositionP | LocationPositionP) | Automatic
    Index Matches to: Source

    RestrictSource

    Indicates whether the source should be restricted from automatic use is any of your team's experiments that request the sample's models. (Restricted samples can only be used in experiments when specifically provided as input to the experiment functions by a team member.)
    Default Value: Automatic
    Default Calculation: If this sample is being reused as a source/destination elsewhere in your Transfer and the RestrictSource/RestrictDestination option are specified for that sample, the same value will be used.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    RestrictDestination

    Indicates whether the destination should be restricted from automatic use is any of your team's experiments that request the sample's models. (Restricted samples can only be used in experiments when specifically provided as input to the experiment functions by a team member.)
    Default Value: Automatic
    Default Calculation: If this sample is being reused as a source/destination elsewhere in your Transfer and the RestrictSource/RestrictDestination option are specified for that sample, the same value will be used.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    DestinationWell

    The position in the destination container in which the source sample will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to the position of the given Object[Sample]. Otherwise, is set to the first empty well of the container. Otherwise, is set to "A1".
    Pattern Description: Any valid container position or any well from A1 to H12.
    Programmatic Pattern: (WellPositionP | LocationPositionP) | Automatic
    Index Matches to: Source

    MultichannelTransfer

    Indicates if this transfer will occur simultaneously with the other transfer that occur before/after it, up to the number of channels that are available in the pipette chosen via the Instrument option.
    Default Value: Automatic
    Default Calculation: Automatically set to True if (1) the samples are in a plate, (2) the samples to be transferred before/after this sample are in the same row or well of the plate, (3) the volume of the samples to be transferred is the same, and (4) the spacing of the wells in the plate are compatible with the spacing between the channels in the pipette (HorizontalPitch/VerticalPitch/ChannelOffset).
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    TemperatureConditions

    CoolingTime

    Specifies the length of time that should elapse after the transfer before the sample is removed from the TransferEnvironment. This is a lower bound, which will govern the final transfer, and so any given sample in a batch may cool for longer than the CoolingTime.
    Default Value: Automatic
    Default Calculation: Resolves to 10 minutes when the SourceTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    SolidificationTime

    In a media transfer, the duration of time after transferring the liquid media into incubation plates that they are held at ambient temperature for the media containing gelling agents to solidify before allowing them to be used for experiments.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 day or None or Null.
    Programmatic Pattern: (Null | (RangeP[0*Minute, 1*Day] | None)) | Null
    Index Matches to: Source

    Temperature Conditions

    SourceTemperature

    Indicates the temperature at which the source sample will be at during the transfer. When samples are transferred off of the operator cart and into the TransferEnvironment, they are placed in a portable heater/cooler to get the sample to temperature right before the transfer occurs. Note that this is different than the TransportCondition of the sample. Samples transferred in a biosafety cabinet can have SourceTemperature specified as Cold (approximately 4 Celsius), Ambient, or a specific temperature above 25 Celsius.
    Default Value: Automatic
    Default Calculation: Automatically set to the TransferTemperature or TransportTemperature (whichever is first filled out) of the source sample. Otherwise, is transferred at Ambient temperature.
    Pattern Description: Ambient or Cold or greater than or equal to -20 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, 90*Celsius] | Ambient | Cold)) | Automatic) | Null
    Index Matches to: Source

    SourceEquilibrationTime

    The duration of time for which the samples will be heated/cooled to the target SourceTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if SourceTemperature is not set to Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    MaxSourceEquilibrationTime

    The maximum duration of time for which the samples will be heated/cooled to the target SourceTemperature, if they do not reach the SourceTemperature after SourceEquilibrationTime. MaxSourceEquilibrationTime will only be used if SourceEquilibrationCheck is set, in order to extend the equilibration time past the initial SourceEquilibrationTime if SourceTemperature has not been reached. Performing an equilibration check will require stopping the experiment and verifying the temperature before moving on; this may add experiment time and may result in loss of sample through evaporation, and is only recommended for use in cases where temperature precision or temperature data is required.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 Minute if SourceEquilibrationCheck is set.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    SourceEquilibrationCheck

    The method by which to verify the temperature of the source before the transfer is performed. Performing an equilibration check will require stopping the experiment and verifying the temperature before moving on; this may add experiment time and may result in loss of sample through evaporation, and is only recommended for use in cases where temperature precision or temperature data is required.
    Default Value: Automatic
    Default Calculation: Automatically set to Null.
    Pattern Description: ImmersionThermometer or IRThermometer or Null.
    Programmatic Pattern: ((Null | EquilibrationCheckP) | Automatic) | Null
    Index Matches to: Source

    DestinationTemperature

    Indicates the temperature at which the destination will be at during the transfer. When samples are transferred off of the operator cart and into the TransferEnvironment, they are placed in a portable heater/cooler to get the sample to temperature right before the transfer occurs. Note that this is different than the TransportCondition of the sample. Samples transferred in a biosafety cabinet can have DestinationTemperature specified as Cold (approximately 4 Celsius), Ambient, or a specific temperature above 25 Celsius.
    Default Value: Automatic
    Default Calculation: Automatically set to the TransferTemperature or TransportTemperature (whichever is first filled out) of the destination sample. Otherwise, is transferred at Ambient temperature.
    Pattern Description: Ambient or Cold or greater than or equal to -20 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, 90*Celsius] | Ambient | Cold)) | Automatic) | Null
    Index Matches to: Source

    DestinationEquilibrationTime

    The duration of time for which the samples will be heated/cooled to the target DestinationTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if DestinationTemperature is not set to Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    MaxDestinationEquilibrationTime

    The maximum duration of time for which the samples will be heated/cooled to the target DestinationTemperature, if they do not reach the DestinationTemperature after DestinationEquilibrationTime. MaxDestinationEquilibrationTime will only be used if DestinationEquilibrationCheck is set, in order to extend the equilibration time past the initial DestinationEquilibrationTime if DestinationTemperature has not been reached. Performing an equilibration check will require stopping the experiment and verifying the temperature before moving on; this may add experiment time and may result in loss of sample through evaporation, and is only recommended for use in cases where temperature precision or temperature data is required.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 Minute if DestinationEquilibrationCheck is set.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    DestinationEquilibrationCheck

    The method by which to verify the temperature of the destination before the transfer is performed. Performing an equilibration check will require stopping the experiment and verifying the temperature before moving on; this may add experiment time and may result in loss of sample through evaporation, and is only recommended for use in cases where temperature precision or temperature data is required.
    Default Value: Automatic
    Default Calculation: Automatically set to Null.
    Pattern Description: ImmersionThermometer or IRThermometer or Null.
    Programmatic Pattern: ((Null | EquilibrationCheckP) | Automatic) | Null
    Index Matches to: Source

    Instrument Specifications

    Instrument

    The standalone instrument used to transfer the sample from the source container (or from the intermediate container if IntermediateDecant->True) to the destination container. If this option is set to Null, it indicates that pouring will be done to perform the transfer when Preparation->Manual.
    Figure 3.2: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to an instrument that can move the amount being transferred and the source and destination containers of the transfer. For more information, please refer to the function TransferDevices[].
    Pattern Description: An object of type or subtype Model[Container, Syringe], Model[Container, GraduatedCylinder], Model[Instrument, Pipette], Model[Instrument, Aspirator], Model[Item, Spatula], Model[Item, Tweezer], Model[Item, TransferTube], Model[Item, ChippingHammer], Model[Item, Scissors], Object[Container, Syringe], Object[Container, GraduatedCylinder], Object[Instrument, Pipette], Object[Instrument, Aspirator], Object[Item, Spatula], Object[Item, Tweezer], Object[Item, TransferTube], Object[Item, ChippingHammer], or Object[Item, Scissors] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Model[Container, GraduatedCylinder], Model[Instrument, Pipette], Model[Instrument, Aspirator], Model[Item, Spatula], Model[Item, Tweezer], Model[Item, TransferTube], Model[Item, ChippingHammer], Model[Item, Scissors], Object[Container, Syringe], Object[Container, GraduatedCylinder], Object[Instrument, Pipette], Object[Instrument, Aspirator], Object[Item, Spatula], Object[Item, Tweezer], Object[Item, TransferTube], Object[Item, ChippingHammer], Object[Item, Scissors]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    TransferEnvironment

    The environment in which the transfer will be performed (Biosafety Cabinet, Fume Hood, Glove Box, or Bench). Containers involved in the transfer will first be moved into the TransferEnvironment (with covers on), uncovered inside of the TransferEnvironment, then covered after the Transfer has finished -- before they're moved back onto the operator cart. Consult the SterileTechnique/RNaseFreeTechnique option when using a BSC. This option cannot be set when Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to an instrument that can handle the any safety requirements of the samples being transferred.
    Pattern Description: An object of type or subtype Model[Instrument, BiosafetyCabinet], Model[Instrument, FumeHood], Model[Instrument, GloveBox], Model[Container, Bench], Object[Instrument, BiosafetyCabinet], Object[Instrument, FumeHood], Object[Instrument, GloveBox], or Object[Container, Bench] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, BiosafetyCabinet], Model[Instrument, FumeHood], Model[Instrument, GloveBox], Model[Container, Bench], Object[Instrument, BiosafetyCabinet], Object[Instrument, FumeHood], Object[Instrument, GloveBox], Object[Container, Bench]}]) | Automatic) | Null
    Index Matches to: Source

    Balance

    The balance used to weigh the transfer amount, if the transfer is occurring gravimetrically.
    Default Value: Automatic
    Default Calculation: Automatically set to a balance whose MinWeight and MaxWeight is compatible with the transfer amount to be measured, if the transfer is occurring gravimetrically (MassP).
    Pattern Description: An object of type or subtype Model[Instrument, Balance] or Object[Instrument, Balance] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Balance], Object[Instrument, Balance]}]) | Automatic) | Null
    Index Matches to: Source

    TabletCrusher

    The pill crusher that will be used to crush any itemized source samples if they are being transferred by mass and not by count.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Item, TabletCrusher, "Silent Knight Pill Crusher"] if an Itemized sample is being transferred by mass and not count.
    Pattern Description: An object of type or subtype Model[Item, TabletCrusher] or Object[Item, TabletCrusher] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, TabletCrusher], Object[Item, TabletCrusher]}]) | Automatic) | Null
    Index Matches to: Source

    Tips

    The pipette tips used to aspirate and dispense the requested volume.
    Default Value: Automatic
    Default Calculation: Automatically set to a tip that does not conflict with the incompatible materials of the sample(s) that the tip will come in contact with, the amount being transferred, and the source and destination containers of the transfer (accessibility). For more information, please refer to the function TransferDevices[].
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Source

    TipType

    The type of pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to the TipType field of the calculated Tips that will be used to perform the transfer.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Source

    TipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to the chemistry of the calculated Tips that will be used to perform the transfer.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Source

    AspirationRate

    The speed at which liquid will be drawn up into the pipette tip. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseRate if it is specified, otherwise set to 100 Microliter/Second if Preparation->Robotic.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Source

    DispenseRate

    The speed at which liquid will be expelled from the pipette tip. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationRate if it is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Source

    OverAspirationVolume

    The volume of air drawn into the pipette tip at the end of the aspiration of a liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to OverDispenseVolume if it is specified, otherwise set to 5 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Microliter]) | Automatic) | Null
    Index Matches to: Source

    OverDispenseVolume

    The volume of air blown out at the end of the dispensing of a liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Microliter if Preparation->Robotic.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 300*Microliter]) | Automatic) | Null
    Index Matches to: Source

    AspirationWithdrawalRate

    The speed at which the pipette is removed from the liquid after an aspiration. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseWithdrawalRate if it is specified, otherwise set to 2 Millimeter/Second.
    Pattern Description: Greater than or equal to 0.3 millimeters per second and less than or equal to 160 millimeters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.3*(Millimeter/Second), 160*(Millimeter/Second)]) | Automatic) | Null
    Index Matches to: Source

    DispenseWithdrawalRate

    The speed at which the pipette is removed from the liquid after a dispense. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationWithdrawalRate if it is specified, otherwise set to 2 Millimeter/Second.
    Pattern Description: Greater than or equal to 0.3 millimeters per second and less than or equal to 160 millimeters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.3*(Millimeter/Second), 160*(Millimeter/Second)]) | Automatic) | Null
    Index Matches to: Source

    AspirationEquilibrationTime

    The delay length the pipette waits after aspirating before it is removed from the liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseEquilibrationTime if it is specified, otherwise set to 1 Second.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 9.9 seconds or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, 9.9*Second]) | Automatic) | Null
    Index Matches to: Source

    DispenseEquilibrationTime

    The delay length the pipette waits after dispensing before it is removed from the liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationEquilibrationTime if it is specified, otherwise set to 1 Second.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 9.9 seconds or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, 9.9*Second]) | Automatic) | Null
    Index Matches to: Source

    AspirationMixRate

    The speed at which liquid is aspirated and dispensed in a liquid before it is aspirated. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseMixRate or AspirationRate if either is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Source

    DispenseMixRate

    The speed at which liquid is aspirated and dispensed in a liquid after a dispense. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationMixRate or DispenseRate if either is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Source

    AspirationPosition

    The location from which liquid should be aspirated. Top will aspirate AspirationPositionOffset below the Top of the container, Bottom will aspirate AspirationPositionOffset above the Bottom of the container, LiquidLevel will aspirate AspirationPositionOffset below the liquid level of the sample in the container, and TouchOff will touch the bottom of the container before moving the specified AspirationPositionOffset above the bottom of the container to start aspirate the sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the AspirationPosition in the PipettingMethod if it is specified and Preparation->Robotic, otherwise resolves to TouchOff if Preparation->Robotic.
    Pattern Description: Top, Bottom, LiquidLevel, or TouchOff or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Source

    DispensePosition

    The location from which liquid should be dispensed. Top will dispense DispensePositionOffset below the Top of the container, Bottom will dispense DispensePositionOffset above the Bottom of the container, LiquidLevel will dispense DispensePositionOffset below the liquid level of the sample in the container, and TouchOff will touch the bottom of the container before moving the specified DispensePositionOffset above the bottom of the container to start dispensing the sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the DispensePosition in the PipettingMethod if it is specified and Preparation->Robotic, resolved to Bottom for MxN MultiProbeHead transfers, otherwise resolves to TouchOff if Preparation->Robotic.
    Pattern Description: Top, Bottom, LiquidLevel, or TouchOff or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Source

    AspirationPositionOffset

    The distance from the center of the well that liquid will aspirated. The Z Offset is based on the AspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel), see the AspirationPosition diagram in the help file for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively. When the source container is tilted (via AspirationAngle), the AspirationPositionOffset automatically accounts for the angle of tilt.
    Default Value: Automatic
    Default Calculation: Automatically set to the AspirationPositionOffset field in the pipetting method, if specified. If AspirationAngle is set, automatically set to the left most point in the well since liquid will pool in the direction that the plate is tilted. Otherwise, is set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Source

    AspirationAngle

    The angle that the source container will be tilted during the aspiration of liquid. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 AngularDegree if Preparation->Robotic. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Automatic) | Null
    Index Matches to: Source

    DispensePositionOffset

    The distance from the center of the well that liquid will dispensed. The Z Offset is based on the DispensePosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel), see the DispensePosition diagram in the help file for more information. If an X and Y offset is not specified, the liquid will be dispensed in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively. When the destination container is tilted (via DispenseAngle), the DispensePositionOffset automatically accounts for the angle of tilt.
    Default Value: Automatic
    Default Calculation: Automatically set to the DispensePositionOffset field in the pipetting method, if specified. If DispenseAngle is set, automatically set to the left most point in the well since liquid will pool in the direction that the plate is tilted. Otherwise, is set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Source

    DispenseAngle

    The angle that the destination container will be tilted during the dispensing of liquid. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 AngularDegree if Preparation->Robotic. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Automatic) | Null
    Index Matches to: Source

    CorrectionCurve

    The relationship between a target volume and the corrected volume that needs to be aspirated or dispensed to reach the target volume. The correction curve is derived empirically from the relationship between the target and actual amount of volume transferred when on a specific robotic liquid handler instrument model. It is recommended when building one of these curves to measure the volume of sample transferred gravimetrically to get a more accurate CorrectionCurve. Use the function UploadPipettingMethod to create a new pipetting method for a sample model to have all robotic transfers of this sample model to use the derived CorrectionCurve automatically. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to PipettingMethod if it is specified. Otherwise, is set to Null (no correction curve).
    Pattern Description: List of one or more {Target Volume, Actual Volume} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1250*Microliter]}..}) | Automatic) | Null
    Index Matches to: Source

    PipettingMethod

    The pipetting parameters used to manipulate the source sample. This option can only be set if Preparation->Robotic. If other pipetting options are specified, the parameters from the method here are overwritten.
    Default Value: Automatic
    Default Calculation: Automatically set to the PipettingMethod of the model of the sample if available.
    Pattern Description: An object of type or subtype Model[Method, Pipetting] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Method, Pipetting]]) | Automatic) | Null
    Index Matches to: Source

    DynamicAspiration

    Indicates if droplet formation will be prevented during liquid transfer. This will only be used for solvents that have high vapor pressure. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to the DynamicAspiration field in the pipetting method, if available.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    DeviceChannel

    The channel of the work cell that will be used to perform the transfer (MultiProbeHead | SingleProbe1 | SingleProbe2 | SingleProbe3 | SingleProbe4 | SingleProbe5 | SingleProbe6 | SingleProbe7 | SingleProbe8). This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to SingleProbe1 if MultichannelTransfer->False. Otherwise, set to the appropriate channel to perform the transfer.
    Pattern Description: MultiProbeHead, SingleProbe1, SingleProbe2, SingleProbe3, SingleProbe4, SingleProbe5, SingleProbe6, SingleProbe7, or SingleProbe8 or Null.
    Programmatic Pattern: ((Null | DeviceChannelP) | Automatic) | Null
    Index Matches to: Source

    Needle

    The needle used to aspirate and dispense the requested volume.
    Default Value: Automatic
    Default Calculation: Automatically set to a needle that does not conflict with the incompatible materials of the sample(s) that the tip will come in contact with, the amount being transferred, and the source and destination containers of the transfer (accessibility). For more information, please refer to the function TransferDevices[].
    Pattern Description: An object of type or subtype Model[Item, Needle] or Object[Item, Needle] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Needle], Object[Item, Needle]}]) | Automatic) | Null
    Index Matches to: Source

    Funnel

    The funnel that is used to guide the source sample into the destination container when pouring or using a graduated cylinder.
    Default Value: Automatic
    Default Calculation: Automatically set to a funnel that can fit into the aperture of the destination container if 1) the transfer Instrument is set to Null (pouring)/GraduatedCylinder or 2) liquid is being transferred from a weighing container to the destination (pouring).
    Pattern Description: An object of type or subtype Model[Part, Funnel] or Object[Part, Funnel] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, Funnel], Object[Part, Funnel]}]) | Automatic) | Null
    Index Matches to: Source

    WeighingContainer

    The container that will be placed on the Balance and used to weigh out the specified amount of the source that will be transferred to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to a weigh boat (for solids) or container (for liquids) that can hold the Amount of the transfer specified if transferring gravimetrically (MassP). Otherwise, set to Null.
    Pattern Description: An object of type or subtype Model[Item, WeighBoat], Object[Item, WeighBoat], Model[Container, Vessel], Object[Container, Vessel], Model[Item, Consumable], Object[Item, Consumable], Model[Container, GraduatedCylinder], or Object[Container, GraduatedCylinder] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Item, WeighBoat], Object[Item, WeighBoat], Model[Container, Vessel], Object[Container, Vessel], Model[Item, Consumable], Object[Item, Consumable], Model[Container, GraduatedCylinder], Object[Container, GraduatedCylinder]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    Tolerance

    The allowed tolerance of the weighed source sample from the specified amount requested to be transferred.
    Default Value: Automatic
    Default Calculation: Automatically set to 5X of the ParticleSize (if specified). Otherwise, set to 2X of the Resolution of the balance being used, if the sample being transferred is a solid. If the sample is not being transferred gravimetrically, set to Null.
    Pattern Description: Greater than 0 grams or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Gram]) | Automatic) | Null
    Index Matches to: Source

    WaterPurifier

    The water purifier used to gather the requested water model required for the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to the water purifier that can fulfill the requested water model. For example, automatically set to Model[Instrument, WaterPurifier, "MilliQ Integral 3"] if the source is Model[Sample, "Milli-Q water"]. Otherwise, set to Null if a water model is not requested.
    Pattern Description: An object of type or subtype Model[Instrument, WaterPurifier] or Object[Instrument, WaterPurifier] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, WaterPurifier], Object[Instrument, WaterPurifier]}]) | Automatic) | Null
    Index Matches to: Source

    HandPump

    The hand pump used to get liquid out of the source container.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Part, HandPump, "id:L8kPEjNLDld6"] if the source is in a container with a MaxVolume over 5 Liters and either (1) the transfer instrument used is a graduated cylinder or (2) an intermediate decant is specified.
    Pattern Description: An object of type or subtype Model[Part, HandPump] or Object[Part, HandPump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, HandPump], Object[Part, HandPump]}]) | Automatic) | Null
    Index Matches to: Source

    IntermediateFunnel

    The funnel that is used to guide the source sample into the intermediate container when pouring.
    Default Value: Automatic
    Default Calculation: Automatically set to a funnel that can fit inside the IntermediateContainer, if an IntermediateDecant is required.
    Pattern Description: An object of type or subtype Model[Part, Funnel] or Object[Part, Funnel] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, Funnel], Object[Part, Funnel]}]) | Automatic) | Null
    Index Matches to: Source

    Transfer Technique

    ReversePipetting

    Indicates if additional source sample will be aspirated (past the first stop of the pipette) to reduce the chance of bubble formation when dispensing into the destination position. This option can only be set if Preparation->Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the source or destination sample has the ReversePipetting field set and the transfer is occurring via pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    SlurryTransfer

    Indicates if the source sample should be mixed via pipette until it becomes homogeneous, up to MaxNumberOfAspirationMixes times.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the SampleHandling of the source sample is set to Slurry.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    AspirationMix

    Indicates if the source sample will be mixed immediately before it is transferred into the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    DispenseMix

    Indicates if the destination sample will be mixed immediately after the source sample is transferred into the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other DispenseMix options are set. Otherwise, set to Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    AspirationMixVolume

    The volume that will be repeatedly aspirated and dispensed via pipette from the source sample in order to mix the source sample immediately before the transfer occurs. The same pipette and tips used in the transfer will be used to mix the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/2 the volume of the source sample or the maximum volume of the pipette being used, depending on which value is smaller.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Source

    NumberOfAspirationMixes

    The number of times the source is quickly aspirated and dispensed to mix the source sample immediately before it is transferred into the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 50, 1]) | Automatic) | Null
    Index Matches to: Source

    MaxNumberOfAspirationMixes

    The number of times the source is quickly aspirated and dispensed to mix the source sample immediately before it is transferred into the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 100 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 100, 1]) | Automatic) | Null
    Index Matches to: Source

    DispenseMixVolume

    The volume that will be repeatedly aspirated and dispensed via pipette from the destination sample in order to mix the destination sample immediately after the transfer occurs. The same pipette and tips used in the transfer will be used to mix the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/2 the volume of the destination sample or the maximum volume of the pipette being used, depending on which value is smaller.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Source

    NumberOfDispenseMixes

    The number of times the destination is quickly aspirated and dispensed to mix the destination sample immediately after the source is dispensed.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other DispenseMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 50, 1]) | Automatic) | Null
    Index Matches to: Source

    Supernatant

    Indicates that only top most layer of the source sample will be aspirated when performing the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Magnetization->True. Otherwise, set to False/Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    AspirationLayer

    The layer (counting from the top) of the source sample that will be aspirated from when performing the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if Supernatant->True. Otherwise, is set to Null.
    Pattern Description: Greater than 0. in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterP[0., 1.]) | Automatic) | Null
    Index Matches to: Source

    DestinationLayer

    The layer (counting from the top) of the destination sample that will be dispensed into when performing the transfer.
    Default Value: Null
    Pattern Description: Greater than 0. in increments of 1 or Null.
    Programmatic Pattern: (Null | GreaterP[0., 1.]) | Null
    Index Matches to: Source

    Magnetization

    Indicates if the source container will be put in a magnetized rack to separate out any magnetic components before the transfer is performed.
    Default Value: Automatic
    Default Calculation: Automatically set to False if transferring a liquid. Otherwise, set to Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    MagnetizationTime

    The time that the source sample will be left on the magnetic rack until the magnetic components are settled at the side of the container.
    Default Value: Automatic
    Default Calculation: Automatically set to 15 Second if Magnetization->True. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    MaxMagnetizationTime

    The maximum time that the source sample will be left on the magnetic rack until the magnetic components are settled at the side of the container.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if Magnetization->True. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    MagnetizationRack

    The magnetized rack that the source/intermediate container will be placed in before the transfer is performed.
    Default Value: Automatic
    Default Calculation: Automatically set to a magnetized rack that can hold the source/intermediate container, if Magnetization->True is specified.
    Pattern Description: An object of type or subtype Model[Container, Rack], Object[Container, Rack], Model[Item, MagnetizationRack], or Object[Item, MagnetizationRack] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Rack], Object[Container, Rack], Model[Item, MagnetizationRack], Object[Item, MagnetizationRack]}]) | Automatic) | Null
    Index Matches to: Source

    CollectionContainer

    Specifies the container that is stacked on the bottom of the destination container, before the source sample is transferred into the destination container, in order to collect the sample that flows through from the bottom of the destination container and into the CollectionContainer. This option is only available when Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] if CollectionTime is specified. Otherwise, set to Null.
    Pattern Description: Existing Container or New Container or New Container with Index or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Object[Container, Plate], Model[Container, Plate]}] | _String) | ObjectP[{Model[Container, Plate]}] | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]})) | Automatic) | Null
    Index Matches to: Source

    CollectionTime

    The amount of time that the sample that is transferred into the destination container is allowed to flow through the bottom of the destination plate and into the CollectionContainer (that is stacked on the bottom of the destination container). This option is only available when Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if CollectionContainer is specified, otherwise set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Source

    SterileTechnique

    Indicates if 70% ethanol will be sprayed on all surfaces/containers used during the transfer. This also indicates that sterile instruments and a sterile transfer environments must be used for the transfer. Please consult the ExperimentTransfer documentation for a full diagram of SterileTechnique that is employed by operators.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the samples being transferred contain tissue culture or microbial components.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    RNaseFreeTechnique

    Indicates that RNase free technique will be followed when performing the transfer (spraying RNase away on surfaces, using RNaseFree tips, etc.).
    Default Value: Automatic
    Default Calculation: Automatically set to True if the samples being transferred are RNaseFree->True.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    Quantitative Transfers

    QuantitativeTransfer

    Indicates if additional QuantitativeTransferWashSolution will be used to wash the weigh boat, NumberOfQuantitativeTransferWashes times, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other QuantitativeTransfer options are set. Otherwise, is set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    QuantitativeTransferWashSolution

    The solution that will be used to wash the weigh boat, NumberOfQuantitativeTransferWashes times, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if any of the other QuantitativeTransfer options are set. Otherwise, is set to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    QuantitativeTransferWashVolume

    The volume of the solution that will be used to wash the weigh boat, NumberOfQuantitativeTransferWashes times, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/4 of the MaxVolume of the weigh boat that will be used if any of the other QuantitativeTransfer options are set. Otherwise, is set to Null.
    Pattern Description: Greater than 0 liters or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Liter]) | Automatic) | Null
    Index Matches to: Source

    QuantitativeTransferWashInstrument

    The pipette that will be used to transfer the wash solution to wash the weigh boat, NumberOfQuantitativeTransferWashes times, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to a pipette that can transfer the requested QuantitativeTransferWashVolume. Otherwise, is set to Null.
    Pattern Description: An object of type or subtype Model[Instrument, Pipette] or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Pipette], Object[Instrument, Pipette]}]) | Automatic) | Null
    Index Matches to: Source

    QuantitativeTransferWashTips

    The tips that will be used to transfer the wash solution to wash the weigh boat, NumberOfQuantitativeTransferWashes times, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to tips that can transfer the requested QuantitativeTransferWashVolume. Otherwise, is set to Null.
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Source

    NumberOfQuantitativeTransferWashes

    Indicates the number of washes of the weight boat with QuantitativeTransferWashSolution that will occur, to maximize the amount of solid that is transferred from the weigh boat (after measurement) to the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 2 if any of the other QuantitativeTransfer options are set. Otherwise, is set to Null.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterP[0, 1]) | Automatic) | Null
    Index Matches to: Source

    Hermetic Transfers

    BackfillGas

    The inert gas that is used equalize the pressure in the source's hermetic container while the transfer out of the source's container occurs.
    Default Value: Automatic
    Default Calculation: Automatically set to Nitrogen if the source's container is hermetic and UnsealHermeticSource->False.
    Pattern Description: Nitrogen or Argon or Null.
    Programmatic Pattern: ((Null | (Nitrogen | Argon)) | Automatic) | Null
    Index Matches to: Source

    BackfillNeedle

    The needle used to backfill the source's hermetic container with BackfillGas.
    Default Value: Automatic
    Default Calculation: Automatically set to the same model of Needle that will be used to perform the transfer, if the source's container is hermetic and UnsealHermeticSource->False.
    Pattern Description: An object of type or subtype Model[Item, Needle] or Object[Item, Needle] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Needle], Object[Item, Needle]}]) | Automatic) | Null
    Index Matches to: Source

    UnsealHermeticSource

    Indicates if the source's hermetic container will be unsealed before sample is transferred out of it.
    Default Value: Automatic
    Default Calculation: Automatically set to True if it is indicated that a syringe/needle will not be used to perform the transfer and the source is in a hermetic container. Otherwise, is set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    VentingNeedle

    The needle that is used equalize the pressure in the destination's hermetic container while the transfer into the destination's container occurs.
    Default Value: Automatic
    Default Calculation: Automatically set to the same model of Needle that will be used to perform the transfer, if the destination's container is hermetic and UnsealHermeticDestination->False.
    Pattern Description: An object of type or subtype Model[Item, Needle] or Object[Item, Needle] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Needle], Object[Item, Needle]}]) | Automatic) | Null
    Index Matches to: Source

    UnsealHermeticDestination

    Indicates if the destination's hermetic container will be unsealed before sample is transferred out of it.
    Default Value: Automatic
    Default Calculation: Automatically set to True if it is indicated that a syringe/needle will not be used to perform the transfer and the destination is in a hermetic container. Otherwise, is set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    Tip Rinsing

    TipRinse

    Indicates if the Tips will first be rinsed with a TipRinseSolution before they are used to aspirate from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other TipRinse options are set. Otherwise, is set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    TipRinseSolution

    The solution that the Tips will be rinsed before they are used to aspirate from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if any of the other TipRinse options are set. Otherwise, is set to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    TipRinseVolume

    The volume of the solution that the Tips will be rinsed before they are used to aspirate from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 125% of the volume to be transferred, or the MaxVolume of the Tips (which ever is smaller) if any of the other TipRinse options are set. Otherwise, is set to Null.
    Pattern Description: Greater than 0 liters or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Liter]) | Automatic) | Null
    Index Matches to: Source

    NumberOfTipRinses

    The number of times that the Tips will be rinsed before they are used to aspirate from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any of the other TipRinse options are set. Otherwise, is set to Null.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterP[0, 1]) | Automatic) | Null
    Index Matches to: Source

    Mixing

    AspirationMixType

    The type of mixing that will occur immediately before aspiration from the source container. Swirl has the operator place the container on the surface of the TransferEnvironment and perform NumberOfAspirationMixes clockwise rotations of the container. Pipette performs NumberOfAspirationMixes aspiration/dispense cycle(s) of AspirationMixVolume using a pipette. Tilt changes the angle of the container to (1) 0 AngularDegrees, (2) 10 AngularDegrees, (3) 0 AngularDegrees, a total of NumberOfAspirationMixes times on a Hamilton integrated tilt plate position. Swirl is only available when Preparation->Manual and Tilt is only available when Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Pipette if any of the other AspirationMix options are set and we're using a pipette to do the transfer. Otherwise, set to Null.
    Pattern Description: Swirl, Pipette, or Tilt or Null.
    Programmatic Pattern: ((Null | (Swirl | Pipette | Tilt)) | Automatic) | Null
    Index Matches to: Source

    DispenseMixType

    The type of mixing that will occur immediately after the sample is dispensed into the destination container. Swirl has the operator place the container on the surface of the TransferEnvironment and perform NumberOfDispenseMixes clockwise rotations of the container. Pipette performs NumberOfDispenseMixes aspiration/dispense cycle(s) of DispenseMixVolume using a pipette. Tilt changes the angle of the container to (1) 0 AngularDegrees, (2) 10 AngularDegrees, (3) 0 AngularDegrees, a total of NumberOfDispenseMixes times on a Hamilton integrated tilt plate position. Swirl is only available when Preparation->Manual and Tilt is only available when Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Pipette if any of the other DispenseMix options are set and we're using a pipette to do the transfer. Otherwise, set to Null.
    Pattern Description: Swirl, Pipette, or Tilt or Null.
    Programmatic Pattern: ((Null | (Swirl | Pipette | Tilt)) | Automatic) | Null
    Index Matches to: Source

    Intermediate Decanting

    IntermediateDecant

    Indicates if the source will need to be decanted into an intermediate container in order for the precise amount requested to be transferred via pipette. Intermediate decants are necessary if the container geometry prevents the Instrument from reaching the liquid level of the sample in the container (plus the delta of volume that is to be transferred). The container geometry is automatically calculated from the inverse of the volume calibration function when the container is parameterized upon receiving. This option will be set to Null if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the source is in a container that in a container that is pipette-inaccessible and the Instrument set to perform the transfer is a pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    IntermediateContainer

    The container that the source will be decanted into in order to make the final transfer via pipette into the final destination.
    Default Value: Automatic
    Default Calculation: Automatically set to the PreferredContainer[...] of the volume that is being transferred if IntermediateDecant->True.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    Container Covering

    KeepSourceCovered

    Indicates if the cover on the source container should be "peaked" off when transferred into/out of instead of taken off completely when performing Manual Transfers in order to reduce chances of contamination or minimize light exposure. When performing robotic manipulations, this indicates that the container should be re-covered after any manipulation that uncovers it is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Preparation->Manual. If Preparation->Robotic, set based on the KeepCovered field in Object[Sample]/Object[Container] for the source sample/container, or True if SterileTechnique is True.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    ReplaceSourceCover

    Indicates if the cover on the source container will be replaced at the end of the transfer with a new type of cover. If set to False, the previous cover (or a new instance of the previous cover if the previous cover is not reusable) will be used to cover the container after the transfer occurs.
    Default Value: False
    Default Calculation: Automatically set to True if any of the SourceCover options are specified.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Source

    SourceCover

    The new cover that will be placed on the source container after the transfer occurs. By default, this option is set to Null which indicates that the previous cover will be used.
    Default Value: Automatic
    Default Calculation: Automatically set to a cover that is compatible with the source container if ReplaceSourceCover->True.
    Pattern Description: An object of type or subtype Object[Item, Cap], Object[Item, PlateSeal], Object[Item, Lid], Model[Item, Cap], Model[Item, PlateSeal], or Model[Item, Lid] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[CoverObjectTypes, CoverModelTypes]]) | Automatic) | Null
    Index Matches to: Source

    SourceSeptum

    The new septum that will be placed on the source container after the transfer occurs. By default, this option is set to Null which indicates that the previous septum will be used (if there was previously a septum on the container). This option can only be set if a new SourceCover is to be used and that SourceCover is a Model[Item, Cap]/Object[Item, Cap] that has CoverType->Crimp and SeptumRequired->True.
    Default Value: Automatic
    Default Calculation: Automatically set to a septum that is compatible with the source container if ReplaceSourceCover->True.
    Pattern Description: An object of type or subtype Model[Item, Septum] or Object[Item, Septum] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Septum], Object[Item, Septum]}]) | Automatic) | Null
    Index Matches to: Source

    SourceStopper

    The new stopper that will be placed on the source container after the transfer occurs. By default, this option is set to Null which indicates that the previous stopper will be used (if there was previously a stopper on the container). This option can only be set if a new SourceCover is to be used and that SourceCover is a Model[Item, Cap]/Object[Item, Cap] that has CoverType->Crimp.
    Default Value: Automatic
    Default Calculation: Automatically set to a stopper that is compatible with the source container if ReplaceSourceCover->True.
    Pattern Description: An object of type or subtype Model[Item, Stopper] or Object[Item, Stopper] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Stopper], Object[Item, Stopper]}]) | Automatic) | Null
    Index Matches to: Source

    KeepDestinationCovered

    Indicates if the cover on the destination container should be "peaked" off when transferred into/out of instead of taken off completely when performing Manual Transfers in order to reduce chances of contamination or minimize light exposure. When performing robotic manipulations, this indicates that the container should be re-covered after any manipulation that uncovers it is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Preparation->Manual. If Preparation->Robotic, set based on the KeepCovered field in Object[Sample]/Object[Container] for the destination sample/container, or True if SterileTechnique is True.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Source

    ReplaceDestinationCover

    Indicates if the cover on the destination container will be replaced at the end of the transfer with a new type of cover. If set to False, the previous cover (or a new instance of the previous cover if the previous cover is not reusable) will be used to cover the container after the transfer occurs.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the DestinationCover options are specified.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Source

    DestinationCover

    The new cover that will be placed on the destination container after the transfer occurs. By default, this option is set to Null which indicates that the previous cover will be used.
    Default Value: Automatic
    Default Calculation: Automatically set to a cover that is compatible with the destination container if ReplaceDestinationCover->True.
    Pattern Description: An object of type or subtype Object[Item, Cap], Object[Item, PlateSeal], Object[Item, Lid], Model[Item, Cap], Model[Item, PlateSeal], or Model[Item, Lid] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[CoverObjectTypes, CoverModelTypes]]) | Automatic) | Null
    Index Matches to: Source

    DestinationSeptum

    The new septum that will be placed on the destination container after the transfer occurs. By default, this option is set to Null which indicates that the previous septum will be used (if there was previously a septum on the container). This option can only be set if a new DestinationCover is to be used and that DestinationCover is a Model[Item, Cap]/Object[Item, Cap] that has SeptumRequired->True.
    Default Value: Automatic
    Default Calculation: Automatically set to a septum that is compatible with the destination container if ReplaceDestinationCover->True.
    Pattern Description: An object of type or subtype Model[Item, Septum] or Object[Item, Septum] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Septum], Object[Item, Septum]}]) | Automatic) | Null
    Index Matches to: Source

    DestinationStopper

    The new stopper that will be placed on the destination container after the transfer occurs. By default, this option is set to Null which indicates that the previous stopper will be used (if there was previously a stopper on the container). This option can only be set if a new DestinationCover is to be used and that DestinationCover is a Model[Item, Cap]/Object[Item, Cap] that has CoverType->Crimp.
    Default Value: Automatic
    Default Calculation: Automatically set to a stopper that is compatible with the destination container if ReplaceDestinationCover->True.
    Pattern Description: An object of type or subtype Model[Item, Stopper] or Object[Item, Stopper] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Stopper], Object[Item, Stopper]}]) | Automatic) | Null
    Index Matches to: Source

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Source

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Source

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Incubate

    Sample

    The sample to be incubated.
    Pattern: NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}

    General

    Sample

    The samples that should be incubated.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples that are being incubated, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers of the samples that are being incubated, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    RelativeHumidity

    The amount of water vapor present in air that the samples are exposed to during incubation, relative to the amount needed for saturation.
    Default Value: Automatic
    Default Calculation: Automatically set to 70 Percent if using an environmental chamber with relative humidity control.
    Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent in increments of 1 percent or Null.
    Programmatic Pattern: ((Null | RangeP[0*Percent, 100*Percent, 1*Percent]) | Automatic) | Null
    Index Matches to: Sample

    LightExposure

    The range of wavelengths of light that the incubated samples are exposed to. Only available when incubating the samples in an environmental chamber with UVLight and VisibleLight control.
    Default Value: Automatic
    Default Calculation: Automatically set to UVLight if LightExposureIntensity is in units of Watt/Meter^2, set to VisibleLight if LightExposureIntensity is in units of Lumen/Meter^2, otherwise set to Null.
    Pattern Description: UVLight or VisibleLight or Null.
    Programmatic Pattern: ((Null | EnvironmentalChamberLightTypeP) | Automatic) | Null
    Index Matches to: Sample

    LightExposureIntensity

    The intensity of light that the incubated samples will be exposed to during the course of the incubation. UVLight exposure is measured in Watt/Meter^2 and Visible Light Intensity is measured in Lumen/Meter^2.
    Default Value: Automatic
    Default Calculation: Automatically set to 19 Watt/Meter^2 if LightExposure is set to UVLight and 14500 Lumen/Meter^2 if LightExposure is set to VisibleLight. Otherwise, set to Null.
    Pattern Description: UV Light Intensity or Visible Light Intensity or Null.
    Programmatic Pattern: ((Null | (RangeP[2*(Watt/Meter^2), 36*(Watt/Meter^2)] | RangeP[2*(Lumen/Meter^2), 29000*(Lumen/Meter^2)])) | Automatic) | Null
    Index Matches to: Sample

    TotalLightExposure

    The total exposure of light that the incubated samples are exposed to during the course of the incubation. UVLight exposure is measured in Watt*Hour/Meter^2 and Visible Light exposure is measured in Lumen*Hour/Meter^2.
    Default Value: Automatic
    Default Calculation: Automatically calculated by LightExposureIntensity*Time.
    Pattern Description: UV Light Exposure or Visible Light Exposure or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Watt*(Hour/Meter^2)] | GreaterP[0*Lumen*(Hour/Meter^2)])) | Automatic) | Null
    Index Matches to: Sample

    LightExposureStandard

    During light exposure experiments, a set of samples that are placed in an opaque box to receive identical incubation conditions without exposure to light. This option can only be set if incubating other samples in an environmental stability chamber with light exposure.
    Default Value: Null
    Pattern Description: List of one or more an object of type or subtype Object[Sample] or Object[Container] or a prepared sample entries or Null.
    Programmatic Pattern: {(ObjectP[{Object[Sample], Object[Container]}] | _String)..} | Null

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Protocol

    Thaw

    Indicates if any SamplesIn are incubated until visibly liquid, before they are mixed and/or incubated.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any corresponding Thaw options are set. Otherwise, set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ThawTime

    The minimum interval at which the samples are checked to see if they are thawed.
    Default Value: Automatic
    Default Calculation: If Thaw is set to True, this option is automatically set to the ThawTime field in the Object[Sample], if specified. Otherwise, extra small volume samples (under 10mL) will be thawed for 5 Minutes, small volume samples (under 50mL) will be thawed for 15 Minutes, medium volume samples (under 100mL) will be 30 Minutes, and large volume samples (over 100mL) will be thawed for an hour.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MaxThawTime

    The maximum time for which the sample is allowed to thaw.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Hour if Thaw->True for this sample.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ThawTemperature

    Temperature at which the SamplesIn are incubated for the duration of the thawing.
    Default Value: Automatic
    Default Calculation: Resolves to 40 Celsius if Thaw->True for this sample.
    Pattern Description: Greater than or equal to -20 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[$MinIncubationTemperature, 90*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    ThawInstrument

    The instrument that will be used to thaw this sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the first instrument in the list of compatible instruments found by IncubateDevices with the given SamplesIn and ThawTemperature, if Thaw->True.
    Pattern Description: An object of type or subtype Model[Instrument, HeatBlock] or Object[Instrument, HeatBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, HeatBlock], Object[Instrument, HeatBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample is mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any Mix related options are set. Otherwise, set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically sets based on the container of the sample and the Mix option. Specifically, if Mix is set to False, the option is set to Null. If MixInstrument is specified, the option is set based on the type of the Model[Instrument] or Object[Instrument] of the specified MixInstrument. If MixRate and Time are Null, when MixVolume is Null or larger than 50ml, the option is set to Invert, otherwise set to Pipette. If Amplitude, MaxTemperature, or DutyCycle is not Null, the option is set to Homogenizer. If MixRate is set, the option is set based on the type of the Model[Instrument] of the first instrument found by MixDevices that is compatible with the SamplesIn, Temperature, and MixRate.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Instrument

    The instrument used to perform the Mix and/or Incubation.
    Figure 3.3: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    StirBar

    The stir bar that is used to stir the sample.
    Default Value: Automatic
    Default Calculation: If MixRate is above 1000 RPM, or if no compatible impeller can be found for the given stirrer, StirBar is automatically set to the largest compatible stir bar found. Here, a compatible impeller means that it can fit in the aperture of the sample's container, can reach the bottom of the sample's container, and is compatible with the stir instrument given; a compatible stir bar means that its width can fit in the aperture of the sample's container and that its length is not greater than the InternalDiameter of the sample's container. Otherwise set to Null.
    Pattern Description: An object of type or subtype Model[Part, StirBar] or Object[Part, StirBar] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, StirBar], Object[Part, StirBar]}]) | Automatic) | Null
    Index Matches to: Sample

    Time

    Duration of time for which the samples will be mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute for robotic preparation. For manual preparation, if Thaw is True, set to 0 Minute; else if MaxTime is specified, Time is automatically set to 1/3 of MaxTime; otherwise set to 15 Minute. As a special case in manual preparation, when LightExposure is not Null, Thaw is True, set to 0 Minute; else if TotalLightExposure is specified and LightExposureIntensity is not Null, Time is automatically set to TotalLightExposure/LightExposureIntensity; otherwise set to 1 Hour.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MaxTime

    Maximum duration of time for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Shake, Roll, Vortex or Sonicate.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Hour if MixUntilDissolved is True, when an applicable MixType is chosen, otherwise set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    DutyCycle

    The style how the homogenizer mixes the given sample via pulsation of the sonication horn. This duty cycle is repeated indefinitely until the specified Time/MaxTime has elapsed. This option can only be set when mixing via homogenization.
    Default Value: Automatic
    Default Calculation: Automatically set to {10 Millisecond, 10 Millisecond} if mixing by homogenization.
    Pattern Description: {Time On, Time Off} or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Minute, 60*Hour], RangeP[0*Minute, 60*Hour]}) | Automatic) | Null
    Index Matches to: Sample

    MixRate

    The frequency of rotation used by the mixing instrument to mix the samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM for robotic preparation. For manual preparation, MixRate is set to the average of MinRotationRate and MaxRotationRate of the instrument.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    MixRateProfile

    The frequency of rotation of the mixing instrument used to mix the samples, over the course of time.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, MixRate} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration]}..}) | Automatic) | Null
    Index Matches to: Sample

    NumberOfMixes

    The number of times the samples are mixed if MixType of Pipette or Invert is chosen.
    Default Value: Automatic
    Default Calculation: If MaxNumberOfMixes is specified, automatically set to 1/3 of MaxNumberOfMixes (round to integer); else if MixUntilDissolved->True, automatically set to 25; otherwise set to 15.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    MaxNumberOfMixes

    The maximum number of times for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Pipette or Invert.
    Default Value: Automatic
    Default Calculation: When MixUntilDissolved->True, automatically set to 2*NumberOfMixes if it is Numeric (specified or automatically set); if not, set to 50.
    Pattern Description: Greater than or equal to 1 and less than or equal to 250 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 250, 1]) | Automatic) | Null
    Index Matches to: Sample

    MixVolume

    The volume of the sample that is pipetted up and down to mix if MixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, automatically set to 970 Microliter or sample volume informed by the field Volume of the sample, whichever is smaller. For manual preparation, automatically set to 50 Milliliter or half of the sample volume informed by the field Volume of the sample, whichever is smaller.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample

    Temperature

    The temperature of the device that is used to mix/incubate the sample. If mixing via homogenization, the pulse duty cycle of the sonication horn is automatically adjusted if the measured temperature of the sample exceeds this set temperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 40 Celsius if AnnealingTime is specified, if not, set to Ambient.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    TemperatureProfile

    The temperature of the device, over the course of time, that is used to mix/incubate the sample.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, Temperature} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinTemperatureProfileTemperature, $MaxTemperatureProfileTemperature]}..}) | Automatic) | Null
    Index Matches to: Sample

    MaxTemperature

    The maximum temperature that the sample is allowed to reach during mixing via homogenization or sonication. If the measured temperature is above this MaxTemperature, the homogenizer/sonicator turns off until the measured temperature is 2C below the MaxTemperature, then it automatically resumes.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees Celsius and less than or equal to 100 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[0*Celsius, 100*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    OscillationAngle

    The angle of oscillation of the mixing motion when a wrist action shaker is used.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 15 degrees or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 15*AngularDegree]) | Automatic) | Null
    Index Matches to: Sample

    Amplitude

    The amplitude of the sonication horn when mixing via homogenization. When using a microtip horn (ex. for 2mL and 15mL tubes), the maximum amplitude is 70 Percent, as specified by the manufacturer, in order not to damage the instrument.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 10 percent and less than or equal to 100 percent or Null.
    Programmatic Pattern: ((Null | RangeP[10*Percent, 100*Percent]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the Time has passed.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Minute (or to Null in cases where the sample is not being incubated).
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MixFlowRate

    The speed at which liquid is aspirated and dispensed in a liquid before it is aspirated. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 100 Microliter/Second when mixing by pipetting if Preparation->Robotic.
    Pattern Description: MixFlowRate speed at which liquid should be drawn up into the pipette tip or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    MixPosition

    The location from which liquid should be mixed by pipetting. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to LiquidLevel if MixType->Pipette and Preparation->Robotic.
    Pattern Description: MixPosition location from which liquid should be aspirated or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Sample

    MixPositionOffset

    The distance from the center of the well that liquid will aspirated/dispensed while mixing the sample. When specifying a Z Offset, the Z Offset is calculated either as the height below the top of the well, the height above the bottom of the well, or the height below the detected liquid level, depending on value of the AspirationPosition option (Top|Bottom|LiquidLevel). When an AspirationAngle is specified, the AspirationPositionOffset is measured in the frame of reference of the tilted labware (so that wells that are further away from the pivot point of the tilt are in the same frame of reference as wells that are close to the pivot point of the tilt). This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 2 Millimeter if MixType->Pipette and Preparation->Robotic.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample

    MixTiltAngle

    The angle that the sample's container is tilted during the mixing of the sample. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: (Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Null
    Index Matches to: Sample

    CorrectionCurve

    The relationship between a target volume and the corrected volume that needs to be aspirated or dispensed to reach the target volume. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to correction curve set in the PipettingMethod for the Object[Sample] if MixType->Pipette and Preparation->Robotic.
    Pattern Description: List of one or more {Target Volume, Actual Volume} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1250*Microliter]}..}) | Automatic) | Null
    Index Matches to: Sample

    Tips

    The pipette tips used to aspirate and dispense the requested volume..
    Default Value: Automatic
    Default Calculation: If MixType->Pipette, automatically set to the preferred tips determined by TransferDevices with given MixVolume, as well as TipType and TipMaterial if specified, and it is also checked that the tips can reach the bottom of the container.
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Sample

    TipType

    The tip type to use to mix liquid in the manipulation.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field TipType of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample

    TipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field Material of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample

    MultichannelMix

    Indicates if multiple device channels should be used when performing pipette mixing.
    Default Value: Automatic
    Default Calculation: Automatically set to True if there are multiple samples that are mixed via pipette, if MixType->Pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    DeviceChannel

    The channel of the work cell that should be used to perform the pipetting mixing. This option can only be set if Preparation->Robotic and MixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to SingleProbe1 if MultichannelMix->False. Otherwise, set to the appropriate channel to perform the transfer, if Preparation->Robotic and MixType->Pipette.
    Pattern Description: MultiProbeHead, SingleProbe1, SingleProbe2, SingleProbe3, SingleProbe4, SingleProbe5, SingleProbe6, SingleProbe7, or SingleProbe8 or Null.
    Programmatic Pattern: ((Null | DeviceChannelP) | Automatic) | Null
    Index Matches to: Sample

    ResidualIncubation

    Indicates if the incubation and/or mixing should continue after Time/MaxTime has finished while waiting to progress to the next step in the protocol.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Temperature is non-Ambient and the samples being incubated have non-ambient TransportTemperature.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResidualTemperature

    The temperature at which the sample(s) should remain incubating after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Temperature if ResidualIncubation is True and Preparation->Robotic.
    Pattern Description: Ambient or Null or greater than or equal to 0 degrees Celsius and less than or equal to 105 degrees Celsius.
    Programmatic Pattern: ((RangeP[0*Celsius, 105*Celsius] | (Ambient | Null)) | Automatic) | Null
    Index Matches to: Sample

    ResidualMix

    Indicates that the sample(s) should remain shaking at the ResidualMixRate after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResidualMixRate

    When mixing by shaking, this is the rate at which the sample(s) remains shaking after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to MixRate if ResidualMix is True and Preparation->Robotic.
    Pattern Description: Greater than or equal to 30 revolutions per minute and less than or equal to 2500 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[30*RPM, 2500*RPM]) | Automatic) | Null
    Index Matches to: Sample

    Preheat

    Indicates if the incubation position is brought to Temperature before exposing the Sample to it. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Sample Preparation

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Container]]) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Container]]) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Mix

    Sample

    The sample to be mixed.
    Pattern: NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}

    General

    Sample

    The samples that should be mixed.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples that are being incubated, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers of the samples that are being incubated, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    RelativeHumidity

    The amount of water vapor present in air that the samples are exposed to during incubation, relative to the amount needed for saturation.
    Default Value: Automatic
    Default Calculation: Automatically set to 70 Percent if using an environmental chamber with relative humidity control.
    Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent in increments of 1 percent or Null.
    Programmatic Pattern: ((Null | RangeP[0*Percent, 100*Percent, 1*Percent]) | Automatic) | Null
    Index Matches to: Sample

    LightExposure

    The range of wavelengths of light that the incubated samples are exposed to. Only available when incubating the samples in an environmental chamber with UVLight and VisibleLight control.
    Default Value: Automatic
    Default Calculation: Automatically set to UVLight if LightExposureIntensity is in units of Watt/Meter^2, set to VisibleLight if LightExposureIntensity is in units of Lumen/Meter^2, otherwise set to Null.
    Pattern Description: UVLight or VisibleLight or Null.
    Programmatic Pattern: ((Null | EnvironmentalChamberLightTypeP) | Automatic) | Null
    Index Matches to: Sample

    LightExposureIntensity

    The intensity of light that the incubated samples will be exposed to during the course of the incubation. UVLight exposure is measured in Watt/Meter^2 and Visible Light Intensity is measured in Lumen/Meter^2.
    Default Value: Automatic
    Default Calculation: Automatically set to 19 Watt/Meter^2 if LightExposure is set to UVLight and 14500 Lumen/Meter^2 if LightExposure is set to VisibleLight. Otherwise, set to Null.
    Pattern Description: UV Light Intensity or Visible Light Intensity or Null.
    Programmatic Pattern: ((Null | (RangeP[2*(Watt/Meter^2), 36*(Watt/Meter^2)] | RangeP[2*(Lumen/Meter^2), 29000*(Lumen/Meter^2)])) | Automatic) | Null
    Index Matches to: Sample

    TotalLightExposure

    The total exposure of light that the incubated samples are exposed to during the course of the incubation. UVLight exposure is measured in Watt*Hour/Meter^2 and Visible Light exposure is measured in Lumen*Hour/Meter^2.
    Default Value: Automatic
    Default Calculation: Automatically calculated by LightExposureIntensity*Time.
    Pattern Description: UV Light Exposure or Visible Light Exposure or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Watt*(Hour/Meter^2)] | GreaterP[0*Lumen*(Hour/Meter^2)])) | Automatic) | Null
    Index Matches to: Sample

    LightExposureStandard

    During light exposure experiments, a set of samples that are placed in an opaque box to receive identical incubation conditions without exposure to light. This option can only be set if incubating other samples in an environmental stability chamber with light exposure.
    Default Value: Null
    Pattern Description: List of one or more an object of type or subtype Object[Sample] or Object[Container] or a prepared sample entries or Null.
    Programmatic Pattern: {(ObjectP[{Object[Sample], Object[Container]}] | _String)..} | Null

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Protocol

    Thaw

    Indicates if any SamplesIn are incubated until visibly liquid, before they are mixed and/or incubated.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any corresponding Thaw options are set. Otherwise, set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ThawTime

    The minimum interval at which the samples are checked to see if they are thawed.
    Default Value: Automatic
    Default Calculation: If Thaw is set to True, this option is automatically set to the ThawTime field in the Object[Sample], if specified. Otherwise, extra small volume samples (under 10mL) will be thawed for 5 Minutes, small volume samples (under 50mL) will be thawed for 15 Minutes, medium volume samples (under 100mL) will be 30 Minutes, and large volume samples (over 100mL) will be thawed for an hour.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MaxThawTime

    The maximum time for which the sample is allowed to thaw.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Hour if Thaw->True for this sample.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ThawTemperature

    Temperature at which the SamplesIn are incubated for the duration of the thawing.
    Default Value: Automatic
    Default Calculation: Resolves to 40 Celsius if Thaw->True for this sample.
    Pattern Description: Greater than or equal to -20 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[$MinIncubationTemperature, 90*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    ThawInstrument

    The instrument that will be used to thaw this sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the first instrument in the list of compatible instruments found by IncubateDevices with the given SamplesIn and ThawTemperature, if Thaw->True.
    Pattern Description: An object of type or subtype Model[Instrument, HeatBlock] or Object[Instrument, HeatBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, HeatBlock], Object[Instrument, HeatBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample is mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any Mix related options are set. Otherwise, set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically sets based on the container of the sample and the Mix option. Specifically, if Mix is set to False, the option is set to Null. If MixInstrument is specified, the option is set based on the type of the Model[Instrument] or Object[Instrument] of the specified MixInstrument. If MixRate and Time are Null, when MixVolume is Null or larger than 50ml, the option is set to Invert, otherwise set to Pipette. If Amplitude, MaxTemperature, or DutyCycle is not Null, the option is set to Homogenizer. If MixRate is set, the option is set based on the type of the Model[Instrument] of the first instrument found by MixDevices that is compatible with the SamplesIn, Temperature, and MixRate.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Instrument

    The instrument used to perform the Mix and/or Incubation.
    Figure 3.4: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    StirBar

    The stir bar that is used to stir the sample.
    Default Value: Automatic
    Default Calculation: If MixRate is above 1000 RPM, or if no compatible impeller can be found for the given stirrer, StirBar is automatically set to the largest compatible stir bar found. Here, a compatible impeller means that it can fit in the aperture of the sample's container, can reach the bottom of the sample's container, and is compatible with the stir instrument given; a compatible stir bar means that its width can fit in the aperture of the sample's container and that its length is not greater than the InternalDiameter of the sample's container. Otherwise set to Null.
    Pattern Description: An object of type or subtype Model[Part, StirBar] or Object[Part, StirBar] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, StirBar], Object[Part, StirBar]}]) | Automatic) | Null
    Index Matches to: Sample

    Time

    Duration of time for which the samples will be mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute for robotic preparation. For manual preparation, if Thaw is True, set to 0 Minute; else if MaxTime is specified, Time is automatically set to 1/3 of MaxTime; otherwise set to 15 Minute. As a special case in manual preparation, when LightExposure is not Null, Thaw is True, set to 0 Minute; else if TotalLightExposure is specified and LightExposureIntensity is not Null, Time is automatically set to TotalLightExposure/LightExposureIntensity; otherwise set to 1 Hour.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MaxTime

    Maximum duration of time for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Shake, Roll, Vortex or Sonicate.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Hour if MixUntilDissolved is True, when an applicable MixType is chosen, otherwise set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    DutyCycle

    The style how the homogenizer mixes the given sample via pulsation of the sonication horn. This duty cycle is repeated indefinitely until the specified Time/MaxTime has elapsed. This option can only be set when mixing via homogenization.
    Default Value: Automatic
    Default Calculation: Automatically set to {10 Millisecond, 10 Millisecond} if mixing by homogenization.
    Pattern Description: {Time On, Time Off} or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Minute, 60*Hour], RangeP[0*Minute, 60*Hour]}) | Automatic) | Null
    Index Matches to: Sample

    MixRate

    The frequency of rotation used by the mixing instrument to mix the samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM for robotic preparation. For manual preparation, MixRate is set to the average of MinRotationRate and MaxRotationRate of the instrument.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    MixRateProfile

    The frequency of rotation of the mixing instrument used to mix the samples, over the course of time.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, MixRate} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration]}..}) | Automatic) | Null
    Index Matches to: Sample

    NumberOfMixes

    The number of times the samples are mixed if MixType of Pipette or Invert is chosen.
    Default Value: Automatic
    Default Calculation: If MaxNumberOfMixes is specified, automatically set to 1/3 of MaxNumberOfMixes (round to integer); else if MixUntilDissolved->True, automatically set to 25; otherwise set to 15.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    MaxNumberOfMixes

    The maximum number of times for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Pipette or Invert.
    Default Value: Automatic
    Default Calculation: When MixUntilDissolved->True, automatically set to 2*NumberOfMixes if it is Numeric (specified or automatically set); if not, set to 50.
    Pattern Description: Greater than or equal to 1 and less than or equal to 250 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 250, 1]) | Automatic) | Null
    Index Matches to: Sample

    MixVolume

    The volume of the sample that is pipetted up and down to mix if MixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, automatically set to 970 Microliter or sample volume informed by the field Volume of the sample, whichever is smaller. For manual preparation, automatically set to 50 Milliliter or half of the sample volume informed by the field Volume of the sample, whichever is smaller.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample

    Temperature

    The temperature of the device that is used to mix/incubate the sample. If mixing via homogenization, the pulse duty cycle of the sonication horn is automatically adjusted if the measured temperature of the sample exceeds this set temperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 40 Celsius if AnnealingTime is specified, if not, set to Ambient.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    TemperatureProfile

    The temperature of the device, over the course of time, that is used to mix/incubate the sample.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, Temperature} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinTemperatureProfileTemperature, $MaxTemperatureProfileTemperature]}..}) | Automatic) | Null
    Index Matches to: Sample

    MaxTemperature

    The maximum temperature that the sample is allowed to reach during mixing via homogenization or sonication. If the measured temperature is above this MaxTemperature, the homogenizer/sonicator turns off until the measured temperature is 2C below the MaxTemperature, then it automatically resumes.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees Celsius and less than or equal to 100 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[0*Celsius, 100*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    OscillationAngle

    The angle of oscillation of the mixing motion when a wrist action shaker is used.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 15 degrees or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 15*AngularDegree]) | Automatic) | Null
    Index Matches to: Sample

    Amplitude

    The amplitude of the sonication horn when mixing via homogenization. When using a microtip horn (ex. for 2mL and 15mL tubes), the maximum amplitude is 70 Percent, as specified by the manufacturer, in order not to damage the instrument.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 10 percent and less than or equal to 100 percent or Null.
    Programmatic Pattern: ((Null | RangeP[10*Percent, 100*Percent]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the Time has passed.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Minute (or to Null in cases where the sample is not being incubated).
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    MixFlowRate

    The speed at which liquid is aspirated and dispensed in a liquid before it is aspirated. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 100 Microliter/Second when mixing by pipetting if Preparation->Robotic.
    Pattern Description: MixFlowRate speed at which liquid should be drawn up into the pipette tip or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    MixPosition

    The location from which liquid should be mixed by pipetting. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to LiquidLevel if MixType->Pipette and Preparation->Robotic.
    Pattern Description: MixPosition location from which liquid should be aspirated or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Sample

    MixPositionOffset

    The distance from the center of the well that liquid will aspirated/dispensed while mixing the sample. When specifying a Z Offset, the Z Offset is calculated either as the height below the top of the well, the height above the bottom of the well, or the height below the detected liquid level, depending on value of the AspirationPosition option (Top|Bottom|LiquidLevel). When an AspirationAngle is specified, the AspirationPositionOffset is measured in the frame of reference of the tilted labware (so that wells that are further away from the pivot point of the tilt are in the same frame of reference as wells that are close to the pivot point of the tilt). This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 2 Millimeter if MixType->Pipette and Preparation->Robotic.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample

    MixTiltAngle

    The angle that the sample's container is tilted during the mixing of the sample. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: (Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Null
    Index Matches to: Sample

    CorrectionCurve

    The relationship between a target volume and the corrected volume that needs to be aspirated or dispensed to reach the target volume. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to correction curve set in the PipettingMethod for the Object[Sample] if MixType->Pipette and Preparation->Robotic.
    Pattern Description: List of one or more {Target Volume, Actual Volume} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1250*Microliter]}..}) | Automatic) | Null
    Index Matches to: Sample

    Tips

    The pipette tips used to aspirate and dispense the requested volume..
    Default Value: Automatic
    Default Calculation: If MixType->Pipette, automatically set to the preferred tips determined by TransferDevices with given MixVolume, as well as TipType and TipMaterial if specified, and it is also checked that the tips can reach the bottom of the container.
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Sample

    TipType

    The tip type to use to mix liquid in the manipulation.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field TipType of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample

    TipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field Material of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample

    MultichannelMix

    Indicates if multiple device channels should be used when performing pipette mixing.
    Default Value: Automatic
    Default Calculation: Automatically set to True if there are multiple samples that are mixed via pipette, if MixType->Pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    DeviceChannel

    The channel of the work cell that should be used to perform the pipetting mixing. This option can only be set if Preparation->Robotic and MixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to SingleProbe1 if MultichannelMix->False. Otherwise, set to the appropriate channel to perform the transfer, if Preparation->Robotic and MixType->Pipette.
    Pattern Description: MultiProbeHead, SingleProbe1, SingleProbe2, SingleProbe3, SingleProbe4, SingleProbe5, SingleProbe6, SingleProbe7, or SingleProbe8 or Null.
    Programmatic Pattern: ((Null | DeviceChannelP) | Automatic) | Null
    Index Matches to: Sample

    ResidualIncubation

    Indicates if the incubation and/or mixing should continue after Time/MaxTime has finished while waiting to progress to the next step in the protocol.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Temperature is non-Ambient and the samples being incubated have non-ambient TransportTemperature.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResidualTemperature

    The temperature at which the sample(s) should remain incubating after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Temperature if ResidualIncubation is True and Preparation->Robotic.
    Pattern Description: Ambient or Null or greater than or equal to 0 degrees Celsius and less than or equal to 105 degrees Celsius.
    Programmatic Pattern: ((RangeP[0*Celsius, 105*Celsius] | (Ambient | Null)) | Automatic) | Null
    Index Matches to: Sample

    ResidualMix

    Indicates that the sample(s) should remain shaking at the ResidualMixRate after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResidualMixRate

    When mixing by shaking, this is the rate at which the sample(s) remains shaking after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to MixRate if ResidualMix is True and Preparation->Robotic.
    Pattern Description: Greater than or equal to 30 revolutions per minute and less than or equal to 2500 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[30*RPM, 2500*RPM]) | Automatic) | Null
    Index Matches to: Sample

    Preheat

    Indicates if the incubation position is brought to Temperature before exposing the Sample to it. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Sample Preparation

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Container]]) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Container]]) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Centrifuge

    centrifugeRules

    The list of key/value pairs that specify the samples and their centrifugation parameters.
    Pattern: {Sample -> ListableP[NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}], Time -> ListableP[GreaterEqualP[0*Minute]], Intensity -> Automatic | Null | GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration], Temperature -> ListableP[Ambient | TemperatureP], Instrument -> ListableP[ObjectP[{Model[Instrument, Centrifuge], Model[Instrument, Centrifuge]}] | Null]}

    General

    Sample

    The samples that should be centrifuged.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples that are being centrifuged, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers of the samples that are being centrifuged, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    Instrument

    The centrifuge that will be used to spin the provided samples.
    Figure 3.5: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to a centrifuge that can attain the specified intensity, time, temperature, and sterility and (if possible) that is compatible with the sample in its current container.
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge]
    Programmatic Pattern: ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic
    Index Matches to: Sample

    RotorGeometry

    Indicates if the provided samples will be spun at a fixed angle or freely pivot. Unique when Preparation -> Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to a FixedAngleRotor if more than one rotors are available that fit the intensity and container constraints.
    Pattern Description: FixedAngleRotor or SwingingBucketRotor or Null.
    Programmatic Pattern: ((Null | RotorGeometryP) | Automatic) | Null
    Index Matches to: Sample

    RotorAngle

    The angle of the samples in the rotor that will be applied to spin the provided samples if ultracentrifuge instrument is selected. Unique when Preparation -> Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to a rotor that could spin samples at specific angle relative to the axis of rotation.
    Pattern Description: Degree23, Degree24, or Degree90 or Null.
    Programmatic Pattern: ((Null | RotorAngleP) | Automatic) | Null
    Index Matches to: Sample

    ChilledRotor

    Indicates if the ultracentrifuge rotor is stored in refrigerator between usage thus it is prechilled on loading into the ultracentrifuge. Unique when Preparation -> Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to true if the ultracentrifuge is utilized at cold temperature.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Rotor

    The centrifuge rotor that will be used to spin the provided samples if ultracentrifuge instrument is selected. Unique when Preparation -> Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to a rotor that can attain the specified intensity, time, temperature, and sterility and (if possible) fits the sample in its current container.
    Pattern Description: An object of type or subtype Model[Container, CentrifugeRotor] or Object[Container, CentrifugeRotor] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, CentrifugeRotor], Object[Container, CentrifugeRotor]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    Intensity

    The rotational speed or the force that will be applied to the samples by centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically resolves to one fifth of the maximum rotational rate of the centrifuge, rounded to the nearest attainable centrifuge precision.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth.
    Programmatic Pattern: (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic
    Index Matches to: Sample

    Time

    The amount of time for which the samples will be centrifuged.
    Default Value: 5 minutes
    Pattern Description: Greater than 0 minutes.
    Programmatic Pattern: GreaterP[0*Minute]
    Index Matches to: Sample

    Temperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged.
    Default Value: Automatic
    Default Calculation: Automatically set to 4 Celsius if ChilledRotor option is set to True.
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius.
    Programmatic Pattern: (Ambient | RangeP[-10*Celsius, 40*Celsius]) | Automatic
    Index Matches to: Sample

    CollectionContainer

    The container that will be stacked on the bottom of the sample's container to collect the filtrate passing through the filter container.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] if the sample is in a filter plate.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {Alternatives[GreaterEqualP[1, 1]], (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Sterile

    Indicates if the protocol should be performed in a sterile environment.
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Protocol

    NumberOfReplicates

    Number of times each of the input samples will be centrifuged using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 1 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[1, 1] | Null

Filter

    Sample

    The sample/container to be filtered.
    Pattern: ListableP[NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}]

    General

    Sample

    The samples that should be filtered.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the sample that goes into the filter, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the sample's container that goes into the filter, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    FiltrateLabel

    A user defined word or phrase used to identify the sample that has gone through the filter, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    FiltrateContainerLabel

    A user defined word or phrase used to identify the container of the sample that has gone through the filter, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    RetentateLabel

    A user defined word or phrase used to identify the sample that was retained on the filter and subsequently collected, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    RetentateContainerLabel

    A user defined word or phrase used to identify the container of the sample that was retained on the filter and subsequently collected, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SampleOutLabel

    A user defined word or phrase used to identify the sample collected at the end of the filtration (either the Filtrate or Retentate), for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the value of RetentateLabel if Target -> Retentate, or FiltrateLabel if Target -> Filtrate.
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    ContainerOutLabel

    A user defined word or phrase used to identify the container of the sample collected at the end of the filtration (either the Filtrate or Retentate), for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the value of RetentateContainerLabel if Target -> Retentate, or FiltrateContainerLabel if Target -> Filtrate.
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    FilterLabel

    A user defined word or phrase used to identify the filter through which the sample is forced, for use in downstream unit operations This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    CollectionContainerLabel

    A user defined word or phrase used to identify the label of the container that will be used to accumulate the filtrate when filtering by Centrifuge or Vacuum and Buchner funnel (if applicable), for use in downstream unit operations This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBufferLabel

    A user defined word or phrase used to identify the sample in which the retentate is resuspended prior to being transferred to RetentateDestinationWell of RetentateContainerOut, for use in downstream unit operations
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBufferContainerLabel

    A user defined word or phrase used to identify the sample in which the retentate is resuspended prior to being transferred to RetentateDestinationWell of RetentateContainerOut.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashBufferLabel

    A user defined word or phrase used to identify the sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different buffers per input sample.
    Default Value: Automatic
    Pattern Description: A string or list of one or more a string entries or Null.
    Programmatic Pattern: ((Null | (_String | {_String..})) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashBufferContainerLabel

    A user defined word or phrase used to identify the container holding the sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different buffers per input sample.
    Default Value: Automatic
    Pattern Description: A string or list of one or more a string entries or Null.
    Programmatic Pattern: ((Null | (_String | {_String..})) | Automatic) | Null
    Index Matches to: Sample

    CollectionContainer

    The container that will be used to accumulate the filtrate when filtering by Centrifuge or Vacuum and Buchner funnel (if applicable).
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    Volume

    The amount of sample to be transferred into the filter (if it is not already there) prior to its filtration. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to All if the filtering robotically, or Null otherwise.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[0*Milliliter, 10*Milliliter])) | Automatic) | Null
    Index Matches to: Sample

    OccludingRetentateContainerLabel

    A user defined word or phrase used to identify the container of the sample that clogged a filter and was subsequently collected, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    WashFlowThroughLabel

    The label of the sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different labels for flow through samples per input sample.
    Default Value: Automatic
    Pattern Description: A string or list of one or more a string or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (_String | {(_String | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    WashFlowThroughContainerLabel

    The label of the container holding sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different labels for containers of flow through samples per input sample.
    Default Value: Automatic
    Pattern Description: A string or list of one or more a string or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (_String | {(_String | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    WashFlowThroughContainer

    The container holding sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different containers of flow through samples per input sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the same value as FiltrateContainerOut if WashRetentate -> True, or Null otherwise.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    WashFlowThroughDestinationWell

    The position in the container holding sample that is run through the retentate and filter after initial filtration prior to retentate collection.
    Default Value: Automatic
    Default Calculation: Automatically set to the same value as FiltrateContainerOut if WashRetentate -> True, or Null otherwise.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (WellPositionP | {(WellPositionP | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Method

    FiltrationType

    The type of dead-end filtration method that should be used to perform the filtration. This option can only be set to AirPressure if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Will be automatically set to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure.
    Programmatic Pattern: FiltrationTypeP | Automatic
    Index Matches to: Sample

    Time

    The amount of time for which the samples will be filtered.
    Default Value: Automatic
    Default Calculation: For Syringe filtration, automatically set to the MaxVolume of the syringe divided by the desired flow rate. This option may be overwritten if both FlowRate and Time options are provided. For other filtration types, automatically set to 5 minutes, or the value of MaxTime, whichever is shorter.
    Pattern Description: Greater than 0 minutes.
    Programmatic Pattern: GreaterP[0*Minute] | Automatic
    Index Matches to: Sample

    FilterUntilDrained

    Indicates if the filtration should be continued until the sample has all been filtered, or up to the MaxTime, in an attempt to filter the entire sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxTime is specified, or if Type is set to PeristalticPump or Vacuum. Automatically set to False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    MaxTime

    Maximum duration of time for which the samples will be filtered, if the FilterUntilDrained option is chosen. Note this option only applies for filtration types: PeristalticPump or Vacuum.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 * Time if Type is PeristalticPump or Vacuum, or Null otherwise.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    Instrument

    The instrument that should be used to perform the filtration. This option can only be set to pressure filter if Preparation->Robotic.
    Figure 3.6: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Will be automatically set to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump], Model[Instrument, PressureManifold], or Object[Instrument, PressureManifold]
    Programmatic Pattern: ObjectP[{Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump], Model[Instrument, PressureManifold], Object[Instrument, PressureManifold]}] | Automatic
    Index Matches to: Sample

    Syringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Automatically set to an syringe appropriate to the volume of sample being filtered.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FlowRate

    The rate at which the syringe pump will dispense liquid from the syringe and through the filter.
    Default Value: Automatic
    Default Calculation: Automatically set to the 0.2 * MaxVolume of the syringe per minute.
    Pattern Description: Greater than or equal to 0.1 milliliters per minute and less than or equal to 20 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0.1*(Milliliter/Minute), 20*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    Sterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Automatically set to True if WorkCell is set to bioSTAR, or if Instrument is set to Model[Instrument, Centrifuge, "HiG4"].
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane on peristaltic pump.
    Default Value: Automatic
    Default Calculation: Automatically set to a housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    Temperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to $AmbientTemperature if filtering type is Centrifuge.
    Pattern Description: Greater than or equal to -10 degrees Celsius or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[-10*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    Pressure

    The target pressure applied to the filter. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 40 PSI if Method -> AirPressure or Null otherwise.
    Pattern Description: Greater than 0 pounds‐force per inch squared or Null.
    Programmatic Pattern: ((Null | GreaterP[0*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Counterweight

    The counterweight to the input container. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to the appropriate model for the weight of the samples if FiltrationType -> Centrifuge, or Null otherwise.
    Pattern Description: An object of type or subtype Model[Item, Counterweight] or Object[Item, Counterweight] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Item, Counterweight], Object[Item, Counterweight]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    Protocol

    Intensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to 2000 GravitationalAcceleration if filtering type is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    Target

    Indicates if the filtrate samples or retentate samples should populate SamplesOut. Note that if set to Retentate, Filtrate will still be collected as well, just not populated in SamplesOut.
    Default Value: Filtrate
    Pattern Description: Filtrate or Retentate.
    Programmatic Pattern: Filtrate | Retentate
    Index Matches to: Sample

    PrewetFilterCentrifugeIntensity

    The rotational speed or force at which the PrewetFilterBuffer will be centrifuged prior to filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to the value of Intensity of centrifuging, or Null otherwise.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FiltrateContainerOut

    The desired container generated samples should be produced in or transferred into by the end of the experiment, with indices indicating grouping of samples in the same plates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container}
    Programmatic Pattern: ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic
    Index Matches to: Sample

    FiltrateDestinationWell

    The desired position in the corresponding FiltrateContainerOut in which the filtrate samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to A1 in containers with only one position. For plates, automatically set to the corresponding value of the FilterPosition option if the CollectionContainer and FiltrateContainerOut are the same. Otherwise, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    Filter Properties

    Filter

    The filter that should be used to remove impurities from the sample.
    Default Value: Automatic
    Default Calculation: Will be automatically set to a filter appropriate for the filtration type and instrument. If the sample is already in a filter, it will not be moved to a new one unless explicitly specified.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Object[Container, Plate, Filter], Model[Container, Vessel, Filter], Object[Container, Vessel, Filter], Model[Item, Filter], Object[Item, Filter], Model[Item, ExtractionCartridge], or Object[Item, ExtractionCartridge] or a prepared sample.
    Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Object[Container, Plate, Filter], Model[Container, Vessel, Filter], Object[Container, Vessel, Filter], Model[Item, Filter], Object[Item, Filter], Model[Item, ExtractionCartridge], Object[Item, ExtractionCartridge]}] | _String) | Automatic
    Index Matches to: Sample

    FilterPosition

    The desired position in the Filter in which the samples should be placed for the filtering.
    Default Value: Automatic
    Default Calculation: If the input sample is already in a filter, automatically set to the current position. Otherwise, selects the first empty position in the Filter according to the order indicated in Flatten[AllWells[]] if using container filters, or Null otherwise.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    MembraneMaterial

    The material from which the filtration membrane should be made of.
    Default Value: Automatic
    Default Calculation: Will be automatically set to PES or to the MembraneMaterial of Filter if it is specified.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB.
    Programmatic Pattern: FilterMembraneMaterialP | Automatic
    Index Matches to: Sample

    PrefilterMembraneMaterial

    The material from which the prefilter filtration membrane should be made of.
    Default Value: Automatic
    Default Calculation: Will be automatically set to GxF if a prefilter pore size is specified.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to .22 Micron or to the PoreSize of Filter if it is specified. Will be automatically set to Null if MolecularWeightCutoff is specified.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    MolecularWeightCutoff

    The molecular weight cutoff of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to Null or to the MolecularWeightCutoff of Filter if it is specified.
    Pattern Description: 3 kilograms per mole, 3. kilograms per mole, 10 kilograms per mole, 10. kilograms per mole, 30 kilograms per mole, 30. kilograms per mole, 40 kilograms per mole, 40. kilograms per mole, 50 kilograms per mole, 50. kilograms per mole, 100 kilograms per mole, 100. kilograms per mole, 300 kilograms per mole, 300. kilograms per mole, 7 kilograms per mole, 7. kilograms per mole, 40 kilograms per mole, 40. kilograms per mole, or 30.8328 kilograms per mole or Null.
    Programmatic Pattern: ((Null | FilterMolecularWeightCutoffP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the prefilter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: Will be automatically set to .45 Micron if a prefilter membrane material is specified.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterStorageCondition

    The conditions under which any filters used by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Will be automatically set to Null if the Filter option is set to a container filter (as in, it will adopt whatever the default storage condition is of that filter), or Disposal if the Filter option is set to an item filter.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample

    WashFlowThroughStorageCondition

    The conditions under which any retentate wash flow through generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to the same value as SamplesOutStorageCondition if WashRetentate -> True, or Null otherwise.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: ((Null | (((SampleStorageTypeP | Disposal) | {((SampleStorageTypeP | Disposal) | (Null | Automatic))..}) | (ObjectP[Model[StorageCondition]] | {(ObjectP[Model[StorageCondition]] | (Null | Automatic))..}))) | Automatic) | Null
    Index Matches to: Sample

    Retentate Collection

    CollectRetentate

    Indicates if the retentate captured by the filter should be retrieved by direct transfer or resuspension in a new buffer. Note that the Filtrate will always be collected as well, and whether the Filtrate or Retentate are the SamplesOut is dictated by the Target option.
    Default Value: Automatic
    Default Calculation: Automatically set to True if RetentateContainerOut, RetentateDestinationWell, ResuspensionVolume, ResuspensionBuffer, RetentateCollectionMethod, ResuspensionCentrifugeIntensity, or ResuspensionCentrifugeTime are specified, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    RetentateCollectionMethod

    Indicates how resuspended retentate should be transferred into RetentateContainerOut.
    Default Value: Automatic
    Default Calculation: Automatically set to Centrifuge if CollectRetentate -> True and using centrifuge filters that can be inverted inside a new tube and centrifuged again in order to collect retentate. In all other cases, if CollectRetentate is True then set to Resuspend, or Null otherwise.
    Pattern Description: Resuspend, Centrifuge, or Transfer or Null.
    Programmatic Pattern: ((Null | (Resuspend | Centrifuge | Transfer)) | Automatic) | Null
    Index Matches to: Sample

    RetentateContainerOut

    The desired container retentate samples should be transferred into after filtering, with indices indicating grouping of samples in the same plates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the ResuspensionVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one. If not collecting the retentate, automatically set to Null.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    RetentateDestinationWell

    The desired position in the corresponding RetentateContainerOut in which the retentate samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells. If not collecting the retentate, automatically set to Null.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CollectOccludingRetentate

    Indicates that if the filter becomes occluded or clogged during the course of filtration, all retentate that cannot be passed through the filter should be collected into the OccludingRetentateContainer. Note that this is currently only done during syringe filtering; for all other filtration types, use the CollectRetentate boolean directly.
    Default Value: Automatic
    Default Calculation: Automatically set to True if FiltrationType is set to Syringe or if OccludingRetentateContainer or OccludingRetentateContainerLabel are specified, and False otherwise.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    OccludingRetentateContainer

    Indicates the container into which the retentate should be transferred if the filter becomes clogged.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if CollectOccludingRetentate is False, and set to the PreferredContainer of the input sample volume if CollectOccludingRetentate is True
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    OccludingRetentateDestinationWell

    The desired position in the corresponding OccludingRetentateContainer in which the occluding retentate samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if CollectOccludingRetentate is False. Automatically set to A1 in containers with only one positionFor plates, fills wells in the order provided by the function AllWells. If not collecting the retentate, automatically set to Null.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionVolume

    The volume of ResuspensionBuffer to be added to the retentate. This amount will subsequently be resuspended and transferred to RetentateDestinationWell of RetentateContainerOut.
    Default Value: Automatic
    Default Calculation: Automatically set to smaller of the MaxVolume of the RetentateContainerOut and the initial volume of the sample. If not collecting the retentate, automatically set to Null.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBuffer

    The desired sample in which the retentate is resuspended prior to being transferred to RetentateDestinationWell of RetentateContainerOut.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if collecting retentate, or Null if not.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    NumberOfResuspensionMixes

    Number of times the retentate should be pipetted if RetentateCollectionMethod -> Transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 if RetentateCollectionMethod -> Transfer, or Null otherwise.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    Retentate Washing

    WashRetentate

    Indicates if the retentate captured by the filter should be washed with a buffer prior to collection.
    Default Value: Automatic
    Default Calculation: Automatically set to True if RetentateWashBuffer, RetentateWashVolume, NumberOfRetentateWashes, RetentateWashTime, or RetentateWashCentrifugeIntensity is specified, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    RetentateWashBuffer

    The sample that is run through the retentate and filter after initial filtration prior to retentate collection. This value can contain one or multiple different buffers per input sample.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if collecting retentate, or Null if not.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or list of one or more an object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | {((ObjectP[{Model[Sample], Object[Sample]}] | _String) | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashVolume

    The amount of RetentateWashBuffer that is run through the retentate and filter after initial filtration prior to retentate collection.
    Default Value: Automatic
    Default Calculation: Automatically set to the initial volume of the input sample if WashRetentate -> True, or Null otherwise.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or list of one or more greater than or equal to 1 microliter and less than or equal to 20 liters or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | {(RangeP[1*Microliter, 20*Liter] | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    NumberOfRetentateWashes

    The number of times to run RetentateWashBuffer through the retentate and filter after initial filtration prior to retentate collection. Note that if this number is greater than 1, all those washes will go into the same WashFlowThroughContainer.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if WashRetentate -> True, or Null otherwise.
    Pattern Description: Greater than or equal to 1 in increments of 1 or list of one or more greater than or equal to 1 in increments of 1 entries or list of one or more Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[1, 1] | {GreaterEqualP[1, 1]..} | {(Null | Automatic)..})) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashDrainTime

    The amount of time for which the samples will be washed with RetentateWashBuffer after initial filtration and prior to retentate collection.
    Default Value: Automatic
    Default Calculation: Automatically set to the value of the Time option if WashRetentate -> True, or Null otherwise.
    Pattern Description: Greater than 0 minutes or list of one or more greater than 0 minutes or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Minute] | {(GreaterP[0*Minute] | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashCentrifugeIntensity

    The rotational speed or force at which the retentate that has been washed with RetentateWashBuffer after initial filtration and prior to retentate collection.
    Default Value: Automatic
    Default Calculation: Will be automatically set to CentrifugeIntensity if Method -> Centrifuge and WashRetentate -> True, or Null otherwise.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | ((GreaterP[0*RPM] | {(GreaterP[0*RPM] | (Null | Automatic))..}) | (GreaterP[0*GravitationalAcceleration] | {(GreaterP[0*GravitationalAcceleration] | (Null | Automatic))..}))) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashMix

    Indicates if after RetentateWashBuffer is added to the retentate, the retentate should be mixed prior to filtering wash buffer out.
    Default Value: Automatic
    Default Calculation: Automatically set to False if CollectRetentate -> True, or Null otherwise.
    Pattern Description: List of one or more Null or Automatic or True or False entries or True or False or Null.
    Programmatic Pattern: ((Null | (BooleanP | {(BooleanP | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    NumberOfRetentateWashMixes

    Number of times the retentate should be pipetted if WashRetentate -> True and RetentateWashMix -> True.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 if WashRetentate -> True and RetentateWashMix -> True, or Null otherwise.
    Pattern Description: Greater than or equal to 1 in increments of 1 or list of one or more greater than or equal to 1 in increments of 1 or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[1, 1] | {(GreaterEqualP[1, 1] | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    RetentateWashPressure

    The target pressure applied to the retentate that has been washed with RetentateWashBuffer after initial filtration and prior to retentate collection. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to the value of the Pressure option if Method -> AirPressure and WashRetentate -> True, or Null otherwise.
    Pattern Description: Greater than 0 pounds‐force per inch squared or list of one or more greater than 0 pounds‐force per inch squared or Null or Automatic entries or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*PSI] | {(GreaterP[0*PSI] | (Null | Automatic))..})) | Automatic) | Null
    Index Matches to: Sample

    Prewetting

    PrewetFilter

    Indicates if the filter should be pre-wet with PrewetFilterBuffer before the input sample is run through it.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other prewetting options are specified or if the filter has StorageBuffer -> True, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    NumberOfFilterPrewettings

    Number of times the filter should be pre-wet with PrewetFilterBuffer before the input sample is run through it. Note that if there is liquid already in the filter, the first iteration will NOT add any buffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 if PrewetFilter -> True and the Filter has StorageBuffer -> True, or 1 if PrewetFilter -> True otherwise, or Null if PrewetFilter -> False.
    Pattern Description: Greater than or equal to 1 and less than or equal to 5 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 5, 1]) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterTime

    Indicates the length of time the PrewetFilterBuffer is run through the filter to pre-wet it.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if FiltrationType is set to Centrifuge, or Null otherwise.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterBufferVolume

    Indicates the amount of PrewetFilterBuffer to run through the filter prior to running the sample through.
    Default Value: Automatic
    Default Calculation: If the filter has StorageBuffer -> True, automatically set to the filter's StorageBufferVolume. If PrewetFilter -> True otherwise, automatically set to the lesser of 5% of the input sample's volume and the MaxVolume of the filter. Otherwise set to Null.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterBuffer

    Indicates the sample to run through the filter prior to running the input sample through the filter. Note that this is only supported if Preparation -> Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to the Solvent field of the input sample, or the model of the input sample if it exists and UsedAsSolvent is set to True, or Milli-Q water if it is not, or Null if PrewetFilter is False.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterBufferLabel

    The label of the sample run through the fitler prior to running the input sample through the filter.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterContainerOut

    The container that is be used to accumulate the filtrate during the prewetting step.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if PrewetFilter is set to False. Otherwise, automatically set to the same container model as the CollectionContainer if it is specified, or the same model as the FiltrateContainerOut otherwise.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PrewetFilterContainerLabel

    The label of the container that is be used to accumulate the filtrate during the prewetting step.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Instrument Specifications

    Tips

    The pipette tips used to aspirate and dispense the requested volume.
    Default Value: Automatic
    Default Calculation: Automatically set to a tip that does not conflict with the incompatible materials of the sample(s) that the tip will come in contact with, the amount being transferred, and the source and destination containers of the transfer (accessibility). For more information, please refer to the function TransferDevices[].
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Sample

    TipType

    The type of pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to the TipType field of the calculated Tips that will be used to perform the transfer.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample

    TipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: Automatically set to the chemistry of the calculated Tips that will be used to perform the transfer.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample

    AspirationRate

    The speed at which liquid will be drawn up into the pipette tip. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseRate if it is specified, otherwise set to 100 Microliter/Second if Preparation->Robotic.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    DispenseRate

    The speed at which liquid will be expelled from the pipette tip. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationRate if it is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    OverAspirationVolume

    The volume of air drawn into the pipette tip at the end of the aspiration of a liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to OverDispenseVolume if it is specified, otherwise set to 5 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Microliter]) | Automatic) | Null
    Index Matches to: Sample

    OverDispenseVolume

    The volume of air blown out at the end of the dispensing of a liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Microliter if Preparation->Robotic.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 300*Microliter]) | Automatic) | Null
    Index Matches to: Sample

    AspirationWithdrawalRate

    The speed at which the pipette is removed from the liquid after an aspiration. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseWithdrawalRate if it is specified, otherwise set to 2 Millimeter/Second.
    Pattern Description: Greater than or equal to 0.3 millimeters per second and less than or equal to 160 millimeters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.3*(Millimeter/Second), 160*(Millimeter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    DispenseWithdrawalRate

    The speed at which the pipette is removed from the liquid after a dispense. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationWithdrawalRate if it is specified, otherwise set to 2 Millimeter/Second.
    Pattern Description: Greater than or equal to 0.3 millimeters per second and less than or equal to 160 millimeters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.3*(Millimeter/Second), 160*(Millimeter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    AspirationEquilibrationTime

    The delay length the pipette waits after aspirating before it is removed from the liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseEquilibrationTime if it is specified, otherwise set to 1 Second.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 9.9 seconds or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, 9.9*Second]) | Automatic) | Null
    Index Matches to: Sample

    DispenseEquilibrationTime

    The delay length the pipette waits after dispensing before it is removed from the liquid. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationEquilibrationTime if it is specified, otherwise set to 1 Second.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 9.9 seconds or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, 9.9*Second]) | Automatic) | Null
    Index Matches to: Sample

    AspirationMixRate

    The speed at which liquid is aspirated and dispensed in a liquid before it is aspirated. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to DispenseMixRate or AspirationRate if either is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    DispenseMixRate

    The speed at which liquid is aspirated and dispensed in a liquid after a dispense. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to AspirationMixRate or DispenseRate if either is specified, otherwise set to 100 Microliter/Second.
    Pattern Description: Greater than or equal to 0.4 microliters per second and less than or equal to 500 microliters per second or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    AspirationPosition

    The location from which liquid should be aspirated. Top will aspirate AspirationPositionOffset below the Top of the container, Bottom will aspirate AspirationPositionOffset above the Bottom of the container, LiquidLevel will aspirate AspirationPositionOffset below the liquid level of the sample in the container, and TouchOff will touch the bottom of the container before moving the specified AspirationPositionOffset above the bottom of the container to start aspirate the sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the AspirationPosition in the PipettingMethod if it is specified and Preparation->Robotic, otherwise resolves to TouchOff if Preparation->Robotic.
    Pattern Description: Top, Bottom, LiquidLevel, or TouchOff or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Sample

    DispensePosition

    The location from which liquid should be dispensed. Top will dispense DispensePositionOffset below the Top of the container, Bottom will dispense DispensePositionOffset above the Bottom of the container, LiquidLevel will dispense DispensePositionOffset below the liquid level of the sample in the container, and TouchOff will touch the bottom of the container before moving the specified DispensePositionOffset above the bottom of the container to start dispensing the sample.
    Default Value: Automatic
    Default Calculation: Automatically set to the DispensePosition in the PipettingMethod if it is specified and Preparation->Robotic, resolved to Bottom for MxN MultiProbeHead transfers, otherwise resolves to TouchOff if Preparation->Robotic.
    Pattern Description: Top, Bottom, LiquidLevel, or TouchOff or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Sample

    AspirationPositionOffset

    The distance from the center of the well that liquid will aspirated. The Z Offset is based on the AspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel), see the AspirationPosition diagram in the help file for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively. When the source container is tilted (via AspirationAngle), the AspirationPositionOffset automatically accounts for the angle of tilt.
    Default Value: Automatic
    Default Calculation: Automatically set to the AspirationPositionOffset field in the pipetting method, if specified. If AspirationAngle is set, automatically set to the left most point in the well since liquid will pool in the direction that the plate is tilted. Otherwise, is set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample

    AspirationAngle

    The angle that the source container will be tilted during the aspiration of liquid. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 AngularDegree if Preparation->Robotic. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Automatic) | Null
    Index Matches to: Sample

    DispensePositionOffset

    The distance from the center of the well that liquid will dispensed. The Z Offset is based on the DispensePosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel), see the DispensePosition diagram in the help file for more information. If an X and Y offset is not specified, the liquid will be dispensed in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively. When the destination container is tilted (via DispenseAngle), the DispensePositionOffset automatically accounts for the angle of tilt.
    Default Value: Automatic
    Default Calculation: Automatically set to the DispensePositionOffset field in the pipetting method, if specified. If DispenseAngle is set, automatically set to the left most point in the well since liquid will pool in the direction that the plate is tilted. Otherwise, is set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample

    DispenseAngle

    The angle that the destination container will be tilted during the dispensing of liquid. The container is pivoted on its left edge when tilting occurs. This option can only be provided if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 AngularDegree if Preparation->Robotic. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees in increments of 1 degree or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 10*AngularDegree, 1*AngularDegree]) | Automatic) | Null
    Index Matches to: Sample

    CorrectionCurve

    The relationship between a target volume and the corrected volume that needs to be aspirated or dispensed to reach the target volume. The correction curve is derived empirically from the relationship between the target and actual amount of volume transferred when on a specific robotic liquid handler instrument model. It is recommended when building one of these curves to measure the volume of sample transferred gravimetrically to get a more accurate CorrectionCurve. Use the function UploadPipettingMethod to create a new pipetting method for a sample model to have all robotic transfers of this sample model to use the derived CorrectionCurve automatically. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to PipettingMethod if it is specified. Otherwise, is set to Null (no correction curve).
    Pattern Description: List of one or more {Target Volume, Actual Volume} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1250*Microliter]}..}) | Automatic) | Null
    Index Matches to: Sample

    PipettingMethod

    The pipetting parameters used to manipulate the source sample. This option can only be set if Preparation->Robotic. If other pipetting options are specified, the parameters from the method here are overwritten.
    Default Value: Automatic
    Default Calculation: Automatically set to the PipettingMethod of the model of the sample if available.
    Pattern Description: An object of type or subtype Model[Method, Pipetting] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Method, Pipetting]]) | Automatic) | Null
    Index Matches to: Sample

    DynamicAspiration

    Indicates if droplet formation will be prevented during liquid transfer. This will only be used for solvents that have high vapor pressure. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to the DynamicAspiration field in the pipetting method, if available.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    DeviceChannel

    The channel of the work cell that will be used to perform the transfer (MultiProbeHead | SingleProbe1 | SingleProbe2 | SingleProbe3 | SingleProbe4 | SingleProbe5 | SingleProbe6 | SingleProbe7 | SingleProbe8). This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to SingleProbe1 if MultichannelTransfer->False. Otherwise, set to the appropriate channel to perform the transfer.
    Pattern Description: MultiProbeHead, SingleProbe1, SingleProbe2, SingleProbe3, SingleProbe4, SingleProbe5, SingleProbe6, SingleProbe7, or SingleProbe8 or Null.
    Programmatic Pattern: ((Null | DeviceChannelP) | Automatic) | Null
    Index Matches to: Sample

    Transfer Technique

    ReversePipetting

    Indicates if additional source sample will be aspirated (past the first stop of the pipette) to reduce the chance of bubble formation when dispensing into the destination position. This option can only be set if Preparation->Manual.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the source or destination sample has the ReversePipetting field set and the transfer is occurring via pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    SlurryTransfer

    Indicates if the source sample should be mixed via pipette until it becomes homogeneous, up to MaxNumberOfAspirationMixes times.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the SampleHandling of the source sample is set to Slurry.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    AspirationMix

    Indicates if the source sample will be mixed immediately before it is transferred into the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    DispenseMix

    Indicates if the destination sample will be mixed immediately after the source sample is transferred into the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other DispenseMix options are set. Otherwise, set to Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AspirationMixVolume

    The volume that will be repeatedly aspirated and dispensed via pipette from the source sample in order to mix the source sample immediately before the transfer occurs. The same pipette and tips used in the transfer will be used to mix the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/2 the volume of the source sample or the maximum volume of the pipette being used, depending on which value is smaller.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfAspirationMixes

    The number of times the source is quickly aspirated and dispensed to mix the source sample immediately before it is transferred into the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    MaxNumberOfAspirationMixes

    The number of times the source is quickly aspirated and dispensed to mix the source sample immediately before it is transferred into the destination.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other AspirationMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 100 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 100, 1]) | Automatic) | Null
    Index Matches to: Sample

    DispenseMixVolume

    The volume that will be repeatedly aspirated and dispensed via pipette from the destination sample in order to mix the destination sample immediately after the transfer occurs. The same pipette and tips used in the transfer will be used to mix the destination sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/2 the volume of the destination sample or the maximum volume of the pipette being used, depending on which value is smaller.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfDispenseMixes

    The number of times the destination is quickly aspirated and dispensed to mix the destination sample immediately after the source is dispensed.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 if any of the other DispenseMix options are set. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

SolidPhaseExtraction

    Sample

    The sample/container to be extracted.
    Pattern: ListableP[NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}]

    General

    Sample

    The samples that should be extracted by SolidPhaseExtraction.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SampleLabel

    The label of the source sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SourceContainerLabel

    The label of the source container that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreFlushingSolutionLabel

    The label of the PreFlushingSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionLabel

    The label of the ConditioningSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionLabel

    The label of the WashingSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionLabel

    The label of the SecondaryWashingSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionLabel

    The label of the TertiaryWashingSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionLabel

    The label of the ElutingSolution that is being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SampleOutLabel

    The label of all collected flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSampleOutLabel

    The label of the collected PreFlushingSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSampleOutLabel

    The label of the collected ConditioningSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    LoadingSampleFlowthroughSampleOutLabel

    The label of the collected LoadingSample flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    WashingSampleOutLabel

    The label of the collected WashingSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSampleOutLabel

    The label of the collected SecondaryWashingSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSampleOutLabel

    The label of the collected TertiaryWashingSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ElutingSampleOutLabel

    The label of the collected ElutingSolution flowthrough sample that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ContainerOutLabel

    The label of the destination container that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ExtractionCartridgeLabel

    The label of the ExtractionCartridge that are being used in the experiment, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ExtractionStrategy

    Strategy for SolidPhaseExtraction, where Positive means analytes of interest are adsorbed on sorbent component. Negative means that impurities adsorb onto sorbent and analytes pass through unretained. See figures...
    Default Value: Positive
    Pattern Description: Positive or Negative or Null.
    Programmatic Pattern: (Null | (Positive | Negative)) | Null
    Index Matches to: Sample

    ExtractionMode

    The strategy used to select the mobile phase and solid support intended to maximally separate impurities from analytes. ReversePhase separates compounds based on polarity. Sorbent material retains non-polar molecules on its surface. NormalPhase separates compounds based on polarity. Sorbent material retains polar molecules on its surface. IonExchange separates compounds based charge. Sorbent material retains charged molecules on its surface. Affinity separates compounds based on "Lock-and-Key" model between molecules and sorbent materials. Sorbent material selectively retains molecules of interest.
    Default Value: Automatic
    Default Calculation: Automatically set to match with solution and ExtractionSorbent selected.
    Pattern Description: NormalPhase, ReversePhase, IonExchange, SizeExclusion, Affinity, or Chiral or Null.
    Programmatic Pattern: ((Null | SeparationModeP) | Automatic) | Null
    Index Matches to: Sample

    ExtractionSorbent

    The material that adsorb analytes or impurities of interest.
    Default Value: Automatic
    Default Calculation: Automatically resolved to C18, unless ExtractionCartridge or ExtractionSorbent is specified.
    Pattern Description: QuaternaryAmmoniumIon, C4, C8, C18, C18Aq, C30, DiVinylBenzene, Biphenyl, Amide, Amine, Polysaccharide, ProteinG, Silica, Carboxylate, AluminaA, AluminaB, AluminaN, Aminopropyl, Diol, Cyanopropyl, Florisil, HLB, WAX, MAX, MCX, WCX, SizeExclusion, Affinity, Chiral, or Null.
    Programmatic Pattern: (SolidPhaseExtractionFunctionalGroupP | Automatic) | Null
    Index Matches to: Sample

    ExtractionCartridge

    The sorbent-packed container that forms the stationary phase of the extraction for each sample pool. Samples within the same pool are added to the same ExtractionCartridge's well or vessel, depends on the type of ExtractionCartridge (this is where pooling occurs in SolidPhaseExtraction).
    Default Value: Automatic
    Default Calculation: Automatically ExtractionCartridge that has C18 ExtractionSorbent, that also fit with the Instrument.
    Pattern Description: An object of type or subtype Model[Container, ExtractionCartridge], Object[Container, ExtractionCartridge], Model[Container, Vessel, Filter], Object[Container, Vessel, Filter], Model[Container, Plate, Filter], or Object[Container, Plate, Filter] or a prepared sample.
    Programmatic Pattern: (ObjectP[{Model[Container, ExtractionCartridge], Object[Container, ExtractionCartridge], Model[Container, Vessel, Filter], Object[Container, Vessel, Filter], Model[Container, Plate, Filter], Object[Container, Plate, Filter]}] | _String) | Automatic
    Index Matches to: Sample

    Instrument

    The Instrument that generate force to drive the fluid through the sorbent during PreFlushing, Conditioning, LoadingSample, Washing and Eluting steps.
    Figure 3.7: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to match the number of SamplesIn, Volume of all mobile phase solution and collection condition. In case that we cannot find the most compatible instrument, it will default to Gilson GX271 LiquidHandler.
    Pattern Description: An object of type or subtype Model[Instrument, LiquidHandler], Object[Instrument, LiquidHandler], Model[Instrument, PressureManifold], Object[Instrument, PressureManifold], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock]
    Programmatic Pattern: ObjectP[{Model[Instrument, LiquidHandler], Object[Instrument, LiquidHandler], Model[Instrument, PressureManifold], Object[Instrument, PressureManifold], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic
    Index Matches to: Sample

    ExtractionMethod

    The type of force that is used to flush fluid or sample through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to match with the chosen Instrument to run SolidPhaseExtraction.
    Pattern Description: Injection, Gravity, Pressure, Vacuum, Pipette, or Centrifuge.
    Programmatic Pattern: SolidPhaseExtractionMethodP | Automatic
    Index Matches to: Sample

    ExtractionTemperature

    The environmental temperature where the Instrument is set up for ExperimentSolidPhaseExtraction to be performed. The solutions' temperture can be different from ExtractionTemperature.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 60 degrees Celsius.
    Programmatic Pattern: RangeP[-20*Celsius, 60*Celsius] | Ambient
    Index Matches to: Sample

    ExtractionCartridgeStorageCondition

    The conditions under which ExtractionCartridges used by this experiment is stored after the protocol is completed.
    Default Value: Disposal
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Sample

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    PreFlushing

    PreFlushing

    Indicates if sorbent should be washed with PreFlushingSolution prior to Conditioning.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample

    PreFlushingSolution

    The solution that is used to wash the sorbent clean of any residues from manufacturing or storage processes, prior to Conditioning.
    Default Value: Automatic
    Default Calculation: Automatically set to match ElutingSolution if ExtractionStrategy is Positive. And automatically set to match SampleIn's Solvent field if ExtractionStrategy is Negative.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionVolume

    The amount of PreFlushingSolution is flushed through the sorbent to remove any residues prior to Conditioning.
    Default Value: Automatic
    Default Calculation: Automatically set to MaxVolume of ExtractionCartridge or MaxVolume of CollectionContainer, which ever is smaller.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionTemperature

    The set temperature that PreFlushingSolution is incubated for PreFlushingTemperatureEquilibrationTime before being flushed through the sorbent. The final temperature of PreFlushingSolution is assumed to equilibrate with the set PreFlushingSolutionTemperature.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Null
    Index Matches to: Sample

    PreFlushingSolutionTemperatureEquilibrationTime

    The amount of time that PreFlushingSolution is incubated to achieve the set PreFlushingTemperature. The final temperature of PreFlushingSolution is assumed to equilibrate with the the set PreFlushingTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 Minutes, if PreFlushingTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectPreFlushingSolution

    Indicates if the PreFlushingSolution is collected after flushed through the sorbent.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in PreFlushing step. The collected volume might be less than PreFlushingSolutionVolume because some of PreFlushingSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to generic container that the instrument can accommodate (point to instrument diagram or make a table).
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionDispenseRate

    The rate at which the PreFlushingSolution is applied to the sorbent by Instrument during Preflushing step.
    Default Value: Automatic
    Default Calculation: Automatically set to 3*Milliliter/Minute.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionDrainTime

    The amount of time for PreFlushingSolution to be flushed through the sorbent. If PreFlushingSolutionUntilDrained is set to True, then PreFlushingSolution is continually flushed through the ExtractionCartridge in cycle of PreFlushingSolutionDrainTime until it is drained entirely. If PreFlushingSolutionUntilDrained is set to False, then PreFlushingSolution is flushed through ExtractionCartridge for PreFlushingSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: If the Volume and Rate is given then it is calculate by Volume/Rate. Otherwise automatically set to 2 minutes, or the value of MaxPreFlushingSolutionDrainTime, whichever is shorter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionUntilDrained

    Indicates if PreFlushingSolution is continually flushed through the cartridge in cycle of every PreFlushingDrainTime until it is drained entirely, or until MaxPreFlushingDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ExtractionMethod are Gravity, Pressure, Vacuum or Centrifuge.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxPreFlushingSolutionDrainTime

    Indicates the maximum amount of time to flush PreFlushingSolution through sorbent. PreFlushingSolution is flushed in cycles of PreFlushingDrainTime until either PreFlushingSolution is entirely drained or MaxPreFlushingDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 time of the maximum of PreFlushingDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush PreFlushingSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 50% of Centrifuge's MaxRotationRate or, if ExtractionMethod is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    PreFlushingSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush PreFlushingSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the PreFlushingSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to 10 PSI. if ExtractionMethod is Pressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Conditioning

    Conditioning

    Indicates if sorbent is equilibrate with ConditioningSolution in order to chemically prepare the sorbent to bind either to analytes if ExtractionStrategy is Positive, or to impurities if ExtractionStrategy is Negative.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample

    ConditioningSolution

    The solution that is flushed through the sorbent in order to chemically prepare the sorbent to bind either to analytes if ExtractionStrategy is Positive, or to impurities if ExtractionStrategy is Negative.
    Default Value: Automatic
    Default Calculation: Automatically set to match with ExtractionSorbent as indicate in Table... .
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionVolume

    The amount of ConditioningSolution that is flushed through the sorbent to chemically prepare it to bind either analytes if ExtractionStrategy is Positive, or impurities if ExtractionStrategy is Negative.
    Default Value: Automatic
    Default Calculation: Automatically set to MaxVolume of ExtractionCartridge or MaxVolume of CollectionContainer, which ever is smaller.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionTemperature

    The set temperature that ConditioningSolution is incubated for ConditioningSolutionTemperatureEquilibrationTime before being flushed through the sorbent. The final temperature of ConditioningSolution is assumed to equilibrate with the set ConditioningSolutionTemperature.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Null
    Index Matches to: Sample

    ConditioningSolutionTemperatureEquilibrationTime

    The amount of time that ConditioningSolution is incubated to achieve the set ConditioningSolutionTemperature. The final temperature of ConditioningSolution is assumed to equilibrate with the the set ConditioningSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 Minutes, if ConditioningSolutionTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectConditioningSolution

    Indicates if the ConditioningSolution is collected and saved after flushing through the sorbent.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in Conditioning step. The collected volume might be less than ConditioningSolutionVolume because some of ConditioningSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to generic container that the instrument can accommodate (point to instrument diagram or make a table).
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionDispenseRate

    The rate at which the ConditioningSolution is applied to the sorbent by Instrument during Conditioning step.
    Default Value: Automatic
    Default Calculation: Automatically set to 3*Milliliter/Minute.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionDrainTime

    The amount of time to set on the Instrument for ConditioningSolution to be flushed through the sorbent. If ConditioningSolutionUntilDrained is set to True, then ConditioningSolution is continually flushed through the ExtractionCartridge in cycle of ConditioningSolutionDrainTime until it is drained entirely. If ConditioningSolutionUntilDrained is set to False, then ConditioningSolution is flushed through ExtractionCartridge for ConditioningSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: If the Volume and Rate is given then it is calculate by Volume/Rate. Otherwise automatically set to 2 minutes, or the value of MaxConditioningSolutionDrainTime, whichever is shorter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionUntilDrained

    Indicates if ConditioningSolution is continually flushed through the cartridge in cycle of ConditioningSolutionDrainTime until it is drained entirely, or until MaxConditioningSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ExtractionMethod are Gravity, Pressure, Vacuum or Centrifuge.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxConditioningSolutionDrainTime

    Indicates the maximum amount of time to flush ConditioningSolution through sorbent. ConditioningSolution is flushed in cycles of every ConditioningSolutionDrainTime until MaxConditioningSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 times of maximum ConditioningSolutionDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush ConditioningSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 50% of Centrifuge's MaxRotationRate or, if ExtractionMethod is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    ConditioningSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush ConditioningSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the ConditioningSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to 10 PSI. Applies to ExtractionMethod : Pressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    LoadingSample

    LoadingSampleVolume

    The amount of each individual input sample that is applied into the sorbent. LoadingSampleVolume is set to All, then all of pooled sample will be loaded in to ExtractionCartridge. If multiple samples are included in the same pool, individual samples are loaded sequentially.
    Default Value: Automatic
    Default Calculation: Automatically set to the whole volume of the sample or MaxVolume of ExtractionCartridge, whichever is smaller. When All is specified, it is set to the smaller of 105% of the volume of the sample or MaxVolume of ExtractionCartridge, to ensure the complete transfer.
    Pattern Description: All or greater than or equal to 0 milliliters and less than or equal to 20 liters.
    Programmatic Pattern: (RangeP[0*Milliliter, $MaxTransferVolume] | All) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuantitativeLoadingSample

    Indicates if each individual sample source containers are rinsed with QuantitativeLoadingSampleSolution, and then that rinsed solution is applied into the sorbent to help ensure that all SampleIn is transferred to the sorbent. Only applies when LoadingSampleVolume is set to All.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuantitativeLoadingSampleSolution

    Solution that is used to rinse each individual sample source containers to ensure that all SampleIn is transferred to the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to ConditioningSolution.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuantitativeLoadingSampleVolume

    The amount of QuantitativeLoadingSampleSolution to added and rinsed source container of each individual sample to ensure that all SampleIn is transferred to the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 20% of volume of each individual samples or 20% of MaxVolume of source container, whichever is smaller, if QuantitativeLoadingSample is set to True.
    Pattern Description: Greater than or equal to 0 milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingSampleTemperature

    The set temperature that individual SampleIn is incubated for LoadingSampleTemperatureEquilibrationTime before being loaded into the sorbent.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingSampleTemperatureEquilibrationTime

    The amount of time that individual samples are incubated at LoadingSampleTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 Minutes, if LoadingSampleTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CollectLoadingSampleFlowthrough

    Indicates if the any material that exit the sorbent is collected while sample is being loaded into the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to True, if ExtractionStrategy is Negative. And automastically set to False, if ExtractionStrategy is Positive.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Automatic
    Index Matches to: Sample

    LoadingSampleFlowthroughContainer

    The container that is used to accumulates any material that exit the sorbent while sample is being loaded into the sorbent. The collected pooled sample flowthrough volume might be less than LoadingSampleVolume because some of SampleIn left in cartrdige (the pooled SampleIn is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to generic container that the instrument can accommodate (point to instrument diagram or make a table).
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    LoadingSampleDispenseRate

    The rate at which individual samples are dispensed into the sorbent during sample loading.
    Default Value: Automatic
    Default Calculation: Automatically set to match with (Table machine).
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    LoadingSampleDrainTime

    The amount of time that the sample is flushed through the sorbent after sample loading.
    Default Value: Automatic
    Default Calculation: If the LoadingSampleVolume and LoadingSampleDispenseRate is given then it is calculate by Volume/Rate. Otherwise automatically set to 2 minutes, or the value of SampleMaxDrainTime, whichever is shorter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic
    Index Matches to: Sample

    LoadingSampleUntilDrained

    Indicates if the sample is continually flushed through the cartridge in cycle of LoadingSampleDrainTime until it is drained entirely, or until MaxSampleDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ExtractionMethod is Gravity, Pressure, Vacuum or Centrifuge.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxLoadingSampleDrainTime

    Indicates the maximum amount of time to flush the sample through sorbent. Sample is flushed in cycles of LoadingSampleDrainTime until either LoadingSampleVolume is entirely drained or MaxLoadingSampleDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 * of the maximum of LoadingSampleDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    LoadingSampleCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush pooled sample through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 50% of Centrifuge's MaxRotationRate or, if ExtractionMethod is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    LoadingSamplePressure

    The target pressure applied to the ExtractionCartridge to flush pooled SampleIn through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the LoadingSamplePressure applies to PreFlushingSolutionPressure, ConditioningSolutionPressure, WashingSolutionPressure and ElutingSolutionPressure as well (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to 10 PSI. Applies to ExtractionMethod : Pressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Washing

    Washing

    Indicates if analyte-bound-sorbent is flushed with WashingSolution to get rid non-specific binding and and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to True, if ExtractionStrategy is Positive
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    WashingSolution

    The solution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to match with ExtractionSorbent as indicate in Table... .
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionVolume

    The amount of WashingSolution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 times of MaxVolume of ExtractionCartridge or 10 times MaxVolume of WashingSolutionCollectionContainer, which ever is smaller.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionTemperature

    The set temperature that WashingSolution is incubated for WashingSolutionTemperatureEquilibrationTime before being flushed through the sorbent. The final temperature of WashingSolution is assumed to equilibrate with the set WashingSolutionTemperature.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Null
    Index Matches to: Sample

    WashingSolutionTemperatureEquilibrationTime

    The amount of time that WashingSolution is incubated to achieve the set WashingSolutionTemperature. The final temperature of WashingSolution is assumed to equilibrate with the the set WashingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 Minutes, if WashingSolutionTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectWashingSolution

    Indicates if the WashingSolution is collected and saved after flushing through the sorbent.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in Washing step. The collected volume might be less than WashingSolutionVolume because some of WashingSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to generic container that the instrument can accommodate (point to instrument diagram or make a table).
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionDispenseRate

    The rate at which the WashingSolution is applied to the sorbent by Instrument during Washing step.
    Default Value: Automatic
    Default Calculation: Automatically set to 3*Milliliter/Minute.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionDrainTime

    The amount of time to set on the Instrument for WashingSolution to be flushed through the sorbent. If WashingSolutionUntilDrained is set to True, then WashingSolution is continually flushed thorugh the ExtractionCartridge in cycle of WashingSolutionDrainTime until it is drained entirely. If WashingSolutionUntilDrained is set to False, then WashingSolution is flushed through ExtractionCartridge for WashingSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: If the Volume and Rate is given then it is calculate by Volume/Rate. Otherwise automatically set to 2 minutes, or the value of MaxWashingSolutionDrainTime, whichever is shorter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionUntilDrained

    Indicates if WashingSolution is continually flushed through the cartridge in cycle of WashingSolutionDrainTime until it is drained entirely, or until MaxWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ExtractionMethod are Gravity, Pressure, Vacuum or Centrifuge.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxWashingSolutionDrainTime

    Indicates the maximum amount of time to flush WashingSolution through sorbent. WashingSolution is flushed in cycles of every WashingSolutionDrainTime until MaxWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 times of maximum WashingSolutionDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush WashingSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 50% of Centrifuge's MaxRotationRate or, if ExtractionMethod is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    WashingSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush WashingSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the WashingSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to 10 PSI. Applies to ExtractionMethod : Pressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashing

    SecondaryWashing

    Indicates if analyte-bound-sorbent is flushed with SecondaryWashingSolution to get rid non-specific binding and and improve extraction purity.
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    SecondaryWashingSolution

    The solution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolution.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionVolume

    The amount of SecondaryWashingSolution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionVolume.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionTemperature

    The set temperature that SecondaryWashingSolution is incubated for SecondaryWashingSolutionTemperatureEquilibrationTime before being flushed through the sorbent. The final temperature of SecondaryWashingSolution is assumed to equilibrate with the set SecondaryWashingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionTemperature.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionTemperatureEquilibrationTime

    The amount of time that SecondaryWashingSolution is incubated to achieve the set SecondaryWashingSolutionTemperature. The final temperature of SecondaryWashingSolution is assumed to equilibrate with the the set SecondaryWashingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionTemperatureEquilibrationTime.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectSecondaryWashingSolution

    Indicates if the SecondaryWashingSolution is collected and saved after flushing through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as CollectionWashingSolution.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in SecondaryWashing step. The collected volume might be less than SecondaryWashingSolutionVolume because some of SecondaryWashingSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionCollectionContainer
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionDispenseRate

    The rate at which the SecondaryWashingSolution is applied to the sorbent by Instrument during SecondaryWashing step.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionDispenseRate.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionDrainTime

    The amount of time to set on the Instrument for SecondaryWashingSolution to be flushed through the sorbent. If SecondaryWashingSolutionUntilDrained is set to True, then SecondaryWashingSolution is continually flushed thorugh the ExtractionCartridge in cycle of SecondaryWashingSolutionDrainTime until it is drained entirely. If SecondaryWashingSolutionUntilDrained is set to False, then SecondaryWashingSolution is flushed through ExtractionCartridge for SecondaryWashingSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionDrainTime.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionUntilDrained

    Indicates if SecondaryWashingSolution is continually flushed through the cartridge in cycle of SecondaryWashingSolutionDrainTime until it is drained entirely, or until MaxSecondaryWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionUntilDrained.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxSecondaryWashingSolutionDrainTime

    Indicates the maximum amount of time to flush SecondaryWashingSolution through sorbent. SecondaryWashingSolution is flushed in cycles of every SecondaryWashingSolutionDrainTime until MaxSecondaryWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as MaxWashingSolutionDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush SecondaryWashingSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionCentrifugeIntensity.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    SecondaryWashingSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush SecondaryWashingSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the SecondaryWashingSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionPressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashing

    TertiaryWashing

    Indicates if analyte-bound-sorbent is flushed with TertiaryWashingSolution to get rid non-specific binding and and improve extraction purity.
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    TertiaryWashingSolution

    The solution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolution.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionVolume

    The amount of TertiaryWashingSolution that is flushed through the analyte-bound-sorbent to get rid of non-specific binding and improve extraction purity.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionVolume.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionTemperature

    The set temperature that TertiaryWashingSolution is incubated for TertiaryWashingSolutionTemperatureEquilibrationTime before being flushed through the sorbent. The final temperature of TertiaryWashingSolution is assumed to equilibrate with the set TertiaryWashingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionTemperature.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionTemperatureEquilibrationTime

    The amount of time that TertiaryWashingSolution is incubated to achieve the set TertiaryWashingSolutionTemperature. The final temperature of TertiaryWashingSolution is assumed to equilibrate with the the set TertiaryWashingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionTemperatureEquilibrationTime.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectTertiaryWashingSolution

    Indicates if the TertiaryWashingSolution is collected and saved after flushing through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as CollectionWashingSolution.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in TertiaryWashing step. The collected volume might be less than TertiaryWashingSolutionVolume because some of TertiaryWashingSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionCollectionContainer
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionDispenseRate

    The rate at which the TertiaryWashingSolution is applied to the sorbent by Instrument during TertiaryWashing step.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionDispenseRate.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionDrainTime

    The amount of time to set on the Instrument for TertiaryWashingSolution to be flushed through the sorbent. If TertiaryWashingSolutionUntilDrained is set to True, then TertiaryWashingSolution is continually flushed thorugh the ExtractionCartridge in cycle of TertiaryWashingSolutionDrainTime until it is drained entirely. If TertiaryWashingSolutionUntilDrained is set to False, then TertiaryWashingSolution is flushed through ExtractionCartridge for TertiaryWashingSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionDrainTime.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionUntilDrained

    Indicates if TertiaryWashingSolution is continually flushed through the cartridge in cycle of TertiaryWashingSolutionDrainTime until it is drained entirely, or until MaxTertiaryWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionUntilDrained.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxTertiaryWashingSolutionDrainTime

    Indicates the maximum amount of time to flush TertiaryWashingSolution through sorbent. TertiaryWashingSolution is flushed in cycles of every TertiaryWashingSolutionDrainTime until MaxTertiaryWashingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as MaxWashingSolutionDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush TertiaryWashingSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionCentrifugeIntensity.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    TertiaryWashingSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush TertiaryWashingSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the TertiaryWashingSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashingSolutionPressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Eluting

    Eluting

    Indicates if sorbent is flushed with ElutingSolution to release bound analyte from the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to True, if ExtractionStrategy is Positive
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    ElutingSolution

    The solution that is used to flush and release bound analyte from the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to match with ExtractionSorbent as indicate in Table... .
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionVolume

    The amount of EluteSolution that is flushed through the sorbent to release analyte from the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 25% of MaxVolume of ExtractionCartridge.
    Pattern Description: Greater than or equal to 0. milliliters and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[0.*Milliliter, $MaxTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionTemperature

    The set temperature that ElutingSolution is incubated for ElutingSolutionTemperatureEquilibrationTime before being loaded into the sorbent.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: (Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Null
    Index Matches to: Sample

    ElutingSolutionTemperatureEquilibrationTime

    The amount of time that ElutingSolution is incubated to achieve the set ElutingSolutionTemperature. The final temperature of ElutingSolution is assumed to equilibrate with the the set ElutingSolutionTemperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 Minutes, if ElutingSolutionTemperature is not Ambient.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    CollectElutingSolution

    Indicates if the ElutingSolution is collected and saved after flushing through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to True, if ExtractionStrategy is Positive.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionCollectionContainer

    The container that is used to accumulates any flow through solution in Eluting step. The collected volume might be less than ElutingSolutionVolume because some of ElutingSolution left in cartrdige (the solution is not purged through the sorbent).
    Default Value: Automatic
    Default Calculation: Automatically set to generic container that the instrument can accommodate (point to instrument diagram or make a table).
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionDispenseRate

    The rate at which the ElutingSolution is applied to the sorbent by Instrument during Eluting step.
    Default Value: Automatic
    Default Calculation: Automatically set to 3*Milliliter/Minute.
    Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 1180 milliliters per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*(Milliliter/Minute), 1180*(Milliliter/Minute)]) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionDrainTime

    The amount of time to set on the Instrument for ElutingSolution to be flushed through the sorbent. If ElutingSolutionUntilDrained is set to True, then ElutingSolution is continually flushed thorugh the ExtractionCartridge in cycle of ElutingSolutionDrainTime until it is drained entirely. If ElutingSolutionUntilDrained is set to False, then ElutingSolution is flushed through ExtractionCartridge for ElutingSolutionDrainTime once.
    Default Value: Automatic
    Default Calculation: If the Volume and Rate is given then it is calculate by Volume/Rate. Otherwise automatically set to 2 minutes, or the value of MaxElutingSolutionDrainTime, whichever is shorter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionUntilDrained

    Indicates if ElutingSolution is continually flushed through the cartridge in cycle of ElutingSolutionDrainTime until it is drained entirely, or until MaxElutingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ExtractionMethod are Gravity, Pressure, Vacuum or Centrifuge.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxElutingSolutionDrainTime

    Indicates the maximum amount of time to flush ElutingSolution through sorbent. ElutingSolution is flushed in cycles of every ElutingSolutionDrainTime until MaxElutingSolutionDrainTime has been reached.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 times of maximum ElutingSolutionDrainTime.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionCentrifugeIntensity

    The rotational speed or gravitational force at which the ExtractionCartridge is centrifuged to flush ElutingSolution through the sorbent.
    Default Value: Automatic
    Default Calculation: Automatically set to 50% of Centrifuge's MaxRotationRate or, if ExtractionMethod is Centrifuge.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, 10000*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    ElutingSolutionPressure

    The target pressure applied to the ExtractionCartridge to flush ElutingSolution through the sorbent. If Instrument is Model[Instrument,PressureManifold,"MPE2"], the ElutingSolutionPressure is set to be LoadingSamplePressure (Pressure of Model[Instrument,PressureManifold,"MPE2"] cannot be changed while the Experiment is running).
    Default Value: Automatic
    Default Calculation: Automatically set to 10 PSI. Applies to ExtractionMethod : Pressure.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 100 pounds‐force per inch squared in increments of 1 pound‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 100*PSI, 1*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Aliquot

    aliquotRules

    The list of key/value pairs describing the source sample, amounts to aliquot, and destinations.
    Pattern: {Source -> {(NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP})..}, Amounts -> {GreaterEqualP[0*Milliliter]..} | {GreaterEqualP[0*Gram] | GreaterEqualP[0, 1]..}, Destinations -> NonSelfContainedSampleP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}}

    General

    Source

    The samples that should be aliquoted.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Source
    Nested Index Matches to: Source

    SourceLabel

    A user defined word or phrase used to identify the aliquot samples, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source
    Nested Index Matches to: Source

    SourceContainerLabel

    A user defined word or phrase used to identify the aliquot sample's containers, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source
    Nested Index Matches to: Source

    Amount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[0.1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Source
    Nested Index Matches to: Source

    TargetConcentration

    The desired final concentration of analyte in the aliquot samples after dilution of aliquots of the input samples with the ConcentratedBuffer and BufferDiluent (or AssayBuffer).
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Source
    Nested Index Matches to: Source

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Source
    Nested Index Matches to: Source

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume, Mass, and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Source

    ContainerOut

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container}
    Programmatic Pattern: ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic
    Index Matches to: Source

    SampleOutLabel

    The label of the sample(s) that become the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    ContainerOutLabel

    The label of the container that holds the sample that becomes the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    DestinationWell

    The desired position in the corresponding ContainerOut in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 for a 96-well plate or from A1 to P24 for a 384-well plate.
    Programmatic Pattern: WellPositionP | Automatic
    Index Matches to: Source

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total AssayVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Source

    ConcentratedBufferLabel

    A user defined word or phrase used to identify the concentrated buffer that is diluted by the BufferDilutionFactor in the final solution, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Source

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, set to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    BufferDiluentLabel

    The label of the buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotVolume and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified and AssayVolume dictates we require a buffer; otherwise, set to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    AssayBufferLabel

    A user defined word or phrase used to identify the buffer added to the aliquoted sample, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    ConsolidateAliquots

    Indicates if aliquots from the same sample with the same target concentration should be prepared in the same ContainerOut. Aliquots from the same source sample not requiring dilution will also be consolidated.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Post Experiment

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Source

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

AbsorbanceSpectroscopy

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RecoupSample

    Indicates if the aliquot used to measure the absorbance should be returned to source container after each reading.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Sample

    ContainerOut

    The desired container generated samples should be transferred into by the end of the experiment, with indices indicating grouping of samples in the same plates.
    Default Value: Automatic
    Default Calculation: Automatically selected from ECL's stocked containers based on the volume of solution being prepared.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BlankLabel

    A user defined word or phrase used to identify the blank samples, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Methods

    Indicates the type of vessel to be used to measure the absorbance of SamplesIn. PlateReaders utilize an open well container that transveres light from top to bottom. Cuvette uses a square container with transparent sides to transverse light from the front to back at a fixed path length. Microfluidic uses small channels to load samples which are then gravity-driven towards chambers where light transverse from top to bottom and measured at a fixed path length.
    Default Value: Automatic
    Default Calculation: Automatically determined based on the instrument that can satisfy the requested options. If all instruments are possible, defaults to Microfluidic.
    Pattern Description: Microfluidic, Cuvette, or PlateReader.
    Programmatic Pattern: AbsorbanceMethodP | Automatic

    Instrument

    The instrument used for proceeding with measuring the absorbance of the samples according to the AbsorbanceMethod.
    Figure 3.8: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: If using Microfluidic Method, set to Model[Instrument, PlateReader, "Lunatic"]; if using Cuvette Method, set to Model[Instrument, Spectrophotometer, "Cary 3500"]; If using PlateReader Method set to Model[Instrument, PlateReader, "FLUOstar Omega"] .
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader], Object[Instrument, PlateReader], Object[Instrument, Spectrophotometer], or Model[Instrument, Spectrophotometer]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader], Object[Instrument, Spectrophotometer], Model[Instrument, Spectrophotometer]}] | Automatic

    MicrofluidicChipLoading

    Indicates whether the SamplesIn are loaded by a robotic liquid handler or manually.
    Default Value: Automatic
    Default Calculation: When using the Microfluidic plate readers, automatically set to Robotic.
    Pattern Description: Robotic or Manual or Null.
    Programmatic Pattern: ((Robotic | Manual) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    The number of times to repeat absorbance reading on each provided sample. If Aliquot -> True, this also indicates the number of times each provided sample will be aliquoted. Note that when using the Lunatic, this indicates the number of independent times a 2 uL aliquot will be put into the Lunatic chips and read, and when using the BMG plate readers, this indicates the number of aliquots of the same sample that will be read.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to 3 if QuantifyConcentration is True for any experiment sample. When using the Lunatic, automatically set to 3 or 2 depending on the total number of the experiment samples and the blank samples if QuantifyConcentration is True. The total number of samples allowed in one Lunatic run is 94. Otherwise automatically set to Null.
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[2, 1] | Automatic) | Null

    Absorbance Measurement

    AcquisitionMix

    When using the Cuvette Method, indicates whether the samples within the cuvette should be mixed with a stir bar during data acquisition.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    StirBar

    When using the Cuvette Method, indicates which model stir bar to be inserted into the cuvette to mix the sample.
    Default Value: Automatic
    Default Calculation: If AcquisitionMix is True, StirBar is automatically specified depending on the cuvette. Otherwise, automatically set to Null.
    Pattern Description: An object of type or subtype Model[Part, StirBar] or Object[Part, StirBar] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, StirBar], Object[Part, StirBar]}]) | Automatic) | Null
    Index Matches to: Sample

    SpectralBandwidth

    When using the Cuvette Method, indicates the physical size of the slit from which light passes out from the monochromator. The narrower the bandwidth, the greater the resolution in measurements.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set 1.0 Nanometer. If using plate reader, set to Null.
    Pattern Description: Greater than or equal to 0.5 nanometers and less than or equal to 5 nanometers or Null.
    Programmatic Pattern: (RangeP[0.5*Nanometer, 5*Nanometer] | Automatic) | Null

    TemperatureMonitor

    When using Cuvette Method (Cary 3500), which device (probe or block) will be used to monitor temperature during the Experiment.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set to CuvetteBlock. If using plate reader, set to Null.
    Pattern Description: CuvetteBlock or ImmersionProbe or Null.
    Programmatic Pattern: ((CuvetteBlock | ImmersionProbe) | Automatic) | Null

    AcquisitionMixRate

    When using the Cuvette Method, indicates the rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
    Default Value: Automatic
    Default Calculation: If AcquisitionMix is True, automatically set AcquisitionMixRate 400 RPM.
    Pattern Description: Greater than or equal to 400 revolutions per minute and less than or equal to 1400 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[400*RPM, 1400*RPM] | Automatic) | Null

    NumberOfReadings

    Number of redundant readings taken by the detector to determine a single averaged absorbance reading.
    Default Value: Automatic
    Default Calculation: If an instrument capable of adjusting NumberOfReadings is selected, resolves to 100. Otherwise resolves to Null
    Pattern Description: Greater than or equal to 1 and less than or equal to 200 or Null.
    Programmatic Pattern: (RangeP[1, 200] | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument will follow as it measures absorbance in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Automatic
    Default Calculation: Resolves to Row if a plate reader capable of adjusting read direction is selected.
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn or Null.
    Programmatic Pattern: (ReadDirectionP | Automatic) | Null

    Quantification

    QuantifyConcentration

    Indicates if the concentration of the samples should be determined. Automatically calls AnalyzeAbsorbanceConcentration to update the concentration of analytes on completion of the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to True if QuantificationWavelength is specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    QuantificationAnalyte

    The substance whose concentration should be determined during this experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[List @@ IdentityModelTypeP]) | Automatic) | Null
    Index Matches to: Sample

    QuantificationWavelength

    Gets the ExtinctionCoefficient for the analyte.
    Default Value: Automatic
    Default Calculation: Automatically set to the ExtinctionCoefficients field of the analyte, and set to Null if QuantifyConcentration is False or Automatic.
    Pattern Description: Greater than or equal to 0 nanometers and less than or equal to 1000 nanometers or Null.
    Programmatic Pattern: ((Null | RangeP[0*Nanometer, 1000*Nanometer]) | Automatic) | Null
    Index Matches to: Sample

    Injections

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during absorbance measurement. The corresponding injection times and volumes can be set with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment are stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    Data Processing

    Blanks

    The object or source used to generate a blank sample (i.e. buffer only, water only, etc.) whose absorbance is subtracted as background from the absorbance readings of the SamplesIn to take accound for any artifacts.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if BlankMeasurement is False. Otherwise, automatically set to the value of Solvent in SamplesIn. If Solvent not specfied, set to Model[Sample, "Milli-Q water"].
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BlankVolumes

    The amount of liquid of the Blank that should be transferred out and used to blank measurements. Set BlankVolume to Null to indicate blanks should be read inside their current containers.
    Default Value: Automatic
    Default Calculation: If BlankMeasurement is True, automatically set to the value of AssayVolume if that was specified, or maximum volume of the container otherwise.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 4000 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 4000*Microliter]) | Automatic) | Null
    Index Matches to: Sample

    BlankAbsorbance

    For each members of SamplesIn, indicates if a corresponding blank measurement, which consists of a separate container (well, chamber, or cuvette) than SampleLink, will be performed, prior to measuring the absorbance of samples. If using Cuvettes, the BlankAbsorbance will be read at the same time as the SampleLink.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Sample Handling

    Temperature

    Indicates the temperature the samples will held at prior to measuring absorbance and during data acquisition within the instrument.
    Default Value: Automatic
    Default Calculation: Sets to Ambient if an instrument is capable of manipulating the temperature of the samples.
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: (RangeP[$AmbientTemperature, 45*Celsius] | Ambient) | Automatic

    EquilibrationTime

    The length of time for which the samples equilibrate at the requested temperature before being read.
    Default Value: Automatic
    Default Calculation: If an instrument is capable setting the temperature of the samples, sets to 0 second when Temperature is set to Ambient. Otherwise, it is set to 5 minutes.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    MoatSize

    Indicates the number of concentric perimeters of wells to fill with MoatBuffer in order to slow evaporation of inner assay samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume to add to to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 300*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the sample to use to fill each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover. When using the Cuvette Method, automatically set to True.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Sampling

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to Matrix if SamplingDimension is specified or Ring if SamplingDistance is specified. Otherwise set to Center.
    Pattern Description: Matrix, Spiral, Ring, or Center or Null.
    Programmatic Pattern: (PlateReaderSamplingP | Automatic) | Null

AbsorbanceIntensity

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RecoupSample

    Indicates if the aliquot used to measure the absorbance should be returned to source container after each reading.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Sample

    ContainerOut

    The desired container generated samples should be transferred into by the end of the experiment, with indices indicating grouping of samples in the same plates.
    Default Value: Automatic
    Default Calculation: Automatically selected from ECL's stocked containers based on the volume of solution being prepared.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BlankLabel

    A user defined word or phrase used to identify the blank samples, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Methods

    Indicates the type of vessel to be used to measure the absorbance of SamplesIn. PlateReaders utilize an open well container that transveres light from top to bottom. Cuvette uses a square container with transparent sides to transverse light from the front to back at a fixed path length. Microfluidic uses small channels to load samples which are then gravity-driven towards chambers where light transverse from top to bottom and measured at a fixed path length.
    Default Value: Automatic
    Default Calculation: If any of the SamplesIn provided has a volume less than 500 Micro Liter, set to microfluidic. Otherwise, if there are less 8 samples, set to Cuvette. If none of options are true, set to PlateReader
    Pattern Description: Microfluidic, Cuvette, or PlateReader.
    Programmatic Pattern: AbsorbanceMethodP | Automatic

    Instrument

    The plate reader used for this absorbance experiment.
    Figure 3.9: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Instrument, PlateReader, "FLUOstar Omega"] if Temperature, EquilibrationTime, or any of the PlateReaderMix options are specified, or Model[Instrument, PlateReader, "Lunatic"] otherwise.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader], Object[Instrument, PlateReader], Object[Instrument, Spectrophotometer], or Model[Instrument, Spectrophotometer]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader], Object[Instrument, Spectrophotometer], Model[Instrument, Spectrophotometer]}] | Automatic

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    The number of times to repeat absorbance reading on each provided sample. If Aliquot -> True, this also indicates the number of times each provided sample will be aliquoted. Note that when using the Lunatic, this indicates the number of independent times a 2 uL aliquot will be put into the Lunatic chips and read, and when using the BMG plate readers, this indicates the number of aliquots of the same sample that will be read.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to 3 if QuantifyConcentration is True for any experiment sample. When using the Lunatic, automatically set to 3 or 2 depending on the total number of the experiment samples and the blank samples if QuantifyConcentration is True. The total number of samples allowed in one Lunatic run is 94. Otherwise automatically set to Null.
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[2, 1] | Automatic) | Null

    Absorbance Measurement

    AcquisitionMix

    When using the Cuvette Method, indicates whether the samples within the cuvette should be mixed with a stir bar during data acquisition.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    StirBar

    When using the Cuvette Method, indicates which model stir bar to be inserted into the cuvette to mix the sample.
    Default Value: Automatic
    Default Calculation: If AcquisitionMix is True, StirBar (model xxxx) must be specified. Automatically set to Null.
    Pattern Description: An object of type or subtype Model[Part, StirBar] or Object[Part, StirBar] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, StirBar], Object[Part, StirBar]}]) | Automatic) | Null
    Index Matches to: Sample

    SpectralBandwidth

    When using the Cuvette Method, indicates the physical size of the slit from which light passes out from the monochromator. The narrower the bandwidth, the greater the resolution in measurements.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set 1.0 Nanometer. If using plate reader, set to Null.
    Pattern Description: Greater than or equal to 0.5 nanometers and less than or equal to 5 nanometers or Null.
    Programmatic Pattern: (RangeP[0.5*Nanometer, 5*Nanometer] | Automatic) | Null

    TemperatureMonitor

    When using Cuvette Method (Cary 3500), indicates which device (probe or block) will be used to monitor temperature during the Experiment.
    Default Value: Automatic
    Default Calculation: When using the Cuvette Method, automatically set to CuvetteBlock. If using plate reader, set to Null.
    Pattern Description: CuvetteBlock or ImmersionProbe or Null.
    Programmatic Pattern: ((CuvetteBlock | ImmersionProbe) | Automatic) | Null

    AcquisitionMixRate

    When using the Cuvette Method, indicates the rate at which the samples within the cuvette should be mixed with a stir bar during data acquisition.
    Default Value: Automatic
    Default Calculation: If AcquisitionMix is True, automatically set AcquisitionMixRate 400 RPM.
    Pattern Description: Greater than or equal to 400 revolutions per minute and less than or equal to 1400 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[400*RPM, 1400*RPM] | Automatic) | Null

    AdjustMixRate

    When using a stir bar, if specified AcquisitionMixRate does not provide a stable or consistent circular rotation of the magnetic bar, indicates if mixing should continue up to MaxStirAttempts in attempts to stir the samples. If stir bar is wiggling, decrease AcquisitionMixRate by AcquisitionMixRateIncrements and check if the stir bar is still wiggling. If it is, decrease by AcquisitionMixRateIncrements again. If still wiggling, repeat decrease until MaxStirAttempts. If the stir bar is not moving/stationary, increase the AcquisitionMixRate by AcquisitionMixRateIncrements and check if the stir bar is still stationary. If it is, increase by AcquisitionMixRateIncrements again. If still stationary, repeat increase until MaxStirAttempts. Mixing will occur during data acquisition. After MaxStirAttempts, if stable stirring was not achieved, StirringError will be set to True in the protocol object.
    Default Value: Automatic
    Default Calculation: Automatically set to True if AcquisitionMix is True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MinAcquisitionMixRate

    Sets the lower limit stirring rate to be decreased to for sample mixing in the cuvette while attempting to mix the samples with a stir bar if AdjustMixRate is True.
    Default Value: Automatic
    Default Calculation: Automatically sets to 20% RPM lower than AcquisitionMixRate if AdjustMixRate is True.
    Pattern Description: Greater than or equal to 400 revolutions per minute and less than or equal to 1400 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[400*RPM, 1400*RPM] | Automatic) | Null

    MaxAcquisitionMixRate

    Sets the upper limit stirring rate to be increased to for sample mixing in the cuvette while attempting to mix the samples with a stir bar if AdjustMixRate is True.
    Default Value: Automatic
    Default Calculation: Automatically sets to 20% RPM greater than AcquisitionMixRate if AdjustMixRate is True.
    Pattern Description: Greater than or equal to 400 revolutions per minute and less than or equal to 1400 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[400*RPM, 1400*RPM] | Automatic) | Null

    AcquisitionMixRateIncrements

    Indicates the value to increase or decrease the mixing rate by in a stepwise fashion while attempting to mix the samples with a stir bar.
    Default Value: Automatic
    Default Calculation: Automatically sets to 50 RPM if AdjustMixRate is True.
    Pattern Description: Greater than or equal to 50 revolutions per minute and less than or equal to 500 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[50*RPM, 500*RPM] | Automatic) | Null

    MaxStirAttempts

    Indicates the maximum number of attempts to mix the samples with a stir bar. One attempt designates each time AdjustMixRate changes the AcquisitionMixRate (i.e. each decrease/increase is equivalent to 1 attempt.
    Default Value: Automatic
    Default Calculation: If AdjustMixRate is True, automatically sets to 10.
    Pattern Description: Greater than or equal to 1 and less than or equal to 40 or Null.
    Programmatic Pattern: (RangeP[1, 40] | Automatic) | Null

    NumberOfReadings

    Number of redundant readings taken by the detector to determine a single averaged absorbance reading.
    Default Value: Automatic
    Default Calculation: If an instrument capable of adjusting NumberOfReadings is selected, resolves to 100. Otherwise resolves to Null
    Pattern Description: Greater than or equal to 1 and less than or equal to 200 or Null.
    Programmatic Pattern: (RangeP[1, 200] | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument will follow as it measures absorbance in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Automatic
    Default Calculation: Resolves to Row if a plate reader capable of adjusting read direction is selected.
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn or Null.
    Programmatic Pattern: (ReadDirectionP | Automatic) | Null

    Optics

    Wavelength

    The specific wavelength(s) which should be used to measure absorbance in the samples.
    Default Value: Automatic
    Default Calculation: Automatically resolves to the shortest wavelength specified in the input samples' ExtinctionCoefficients field, and 260 Nanometer if that field is not populated.
    Pattern Description: Greater than or equal to 200 nanometers and less than or equal to 1000 nanometers.
    Programmatic Pattern: RangeP[200*Nanometer, 1000*Nanometer] | Automatic
    Index Matches to: Sample

    Quantification

    QuantificationAnalyte

    The substance whose concentration should be determined during this experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[List @@ IdentityModelTypeP]) | Automatic) | Null
    Index Matches to: Sample

    QuantifyConcentration

    Indicates if the concentration of the samples should be determined.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if QuantificationWavelength is specified and calling ExperimentAbsorbanceSpectroscopy, and resolves to False if calling ExperimentAbsorbanceIntensity.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    Injections

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during absorbance measurement. The corresponding injection times and volumes can be set with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment are stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    Data Processing

    Blanks

    The object or source used to generate a blank sample (i.e. buffer only, water only, etc.) whose absorbance is subtracted as background from the absorbance readings of the SamplesIn to take accound for any artifacts.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if BlankMeasurement is False. Otherwise, automatically set to the value of Solvent in SamplesIn. If Solvent not specfied, set to Model[Sample, "Milli-Q water"].
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BlankVolumes

    The amount of liquid of the Blank that should be transferred out and used to blank measurements. Set BlankVolume to Null to indicate blanks should be read inside their current containers.
    Default Value: Automatic
    Default Calculation: If BlankMeasurement is True, automatically set to the value of AssayVolume if that was specified, or maximum volume of the container otherwise.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 4000 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 4000*Microliter]) | Automatic) | Null
    Index Matches to: Sample

    BlankAbsorbance

    For each members of SamplesIn, indicates if a corresponding blank measurement, which consists of a separate container (well, chamber, or cuvette) than SampleLink, will be performed, prior to measuring the absorbance of samples. If using Cuvettes, the BlankAbsorbance will be read at the same time as the SampleLink.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Sample Handling

    MicrofluidicChipLoading

    When using Lunatic, indicates if Lunatic Microfluidic Chips are loaded by a robotic liquid handler or manually.
    Default Value: Automatic
    Default Calculation: When using the Lunatic plate readers, automatically set to Robotic. When using the BMG plate reader, automatically set to Null.
    Pattern Description: Robotic or Manual or Null.
    Programmatic Pattern: ((Robotic | Manual) | Automatic) | Null

    Temperature

    Indicates the temperature the samples will held at prior to measuring absorbance and during data acquisition within the instrument.
    Default Value: Automatic
    Default Calculation: Sets to Ambient if an instrument is capable of manipulating the temperature of the samples.
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: (RangeP[$AmbientTemperature, 45*Celsius] | Ambient) | Automatic

    EquilibrationTime

    The length of time for which the samples equilibrate at the requested temperature before being read.
    Default Value: Automatic
    Default Calculation: If an instrument is capable setting the temperature of the samples, sets to 0 second when Temperature is set to Ambient. Otherwise, it is set to 5 minutes.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    MoatSize

    Indicates the number of concentric perimeters of wells to fill with MoatBuffer in order to slow evaporation of inner assay samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume to add to to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 300*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the sample to use to fill each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover. When using the Cuvette Method, automatically set to True.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Sampling

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to Matrix if SamplingDimension is specified or Ring if SamplingDistance is specified. Otherwise set to Center.
    Pattern Description: Matrix, Spiral, Ring, or Center or Null.
    Programmatic Pattern: (PlateReaderSamplingP | Automatic) | Null

AbsorbanceKinetics

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    BlankLabel

    A user defined word or phrase used to identify the blank samples, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Instrument

    The plate reader used to measure absorbance.
    Figure 3.10: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Resolves to the instrument capable of performing the requested sampling. If any instrument can be used, resolves to use the model with the most available instruments in the lab.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    The number of times to repeat absorbance reading on each provided sample. If Aliquot -> True, this also indicates the number of times each provided sample will be aliquoted. Note that when using the Lunatic, this indicates the number of independent times a 2 uL aliquot will be put into the Lunatic chips and read, and when using the BMG plate readers, this indicates the number of aliquots of the same sample that will be read.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to 3 if QuantifyConcentration is True for any experiment sample. When using the Lunatic, automatically set to 3 or 2 depending on the total number of the experiment samples and the blank samples if QuantifyConcentration is True. The total number of samples allowed in one Lunatic run is 94. Otherwise automatically set to Null.
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[2, 1] | Automatic) | Null

    Injections

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during absorbance measurement. The corresponding injection times and volumes can be set with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    TertiaryInjectionSample

    The sample to be injected in the third round of injections. Note that only two unique samples can be injected in total. The corresponding injection times and volumes can be set with TertiaryInjectionTime and TertiaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    TertiaryInjectionVolume

    The amount of the tertiary sample injected in the third round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    QuaternaryInjectionSample

    The sample to be injected in the fourth round of injections. Note that only two unique samples can be injected in total. The corresponding injection times and volumes can be set with QuaternaryInjectionTime and QuaternaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    QuaternaryInjectionVolume

    The amount of the quaternary sample injected in the fourth round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment are stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    PrimaryInjectionTime

    The time at which the first round of injections starts.
    Default Value: Null
    Pattern Description: Greater than or equal to 1 second and less than or equal to 24 hours or Null.
    Programmatic Pattern: RangeP[1*Second, 24*Hour] | Null

    SecondaryInjectionTime

    The time at which the second round of injections starts.
    Default Value: Null
    Pattern Description: Greater than or equal to 1 second and less than or equal to 24 hours or Null.
    Programmatic Pattern: RangeP[1*Second, 24*Hour] | Null

    TertiaryInjectionTime

    The time at which the third round of injections starts.
    Default Value: Null
    Pattern Description: Greater than or equal to 1 second and less than or equal to 24 hours or Null.
    Programmatic Pattern: RangeP[1*Second, 24*Hour] | Null

    QuaternaryInjectionTime

    The time at which the fourth round of injections starts.
    Default Value: Null
    Pattern Description: Greater than or equal to 1 second and less than or equal to 24 hours or Null.
    Programmatic Pattern: RangeP[1*Second, 24*Hour] | Null

    Data Processing

    Blanks

    The object or source used to generate a blank sample (i.e. buffer only, water only, etc.) whose absorbance is subtracted as background from the absorbance readings of the SamplesIn to take accound for any artifacts.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if BlankMeasurement is False. Otherwise, automatically set to the value of Solvent in SamplesIn. If Solvent not specfied, set to Model[Sample, "Milli-Q water"].
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BlankVolumes

    The amount of liquid of the Blank that should be transferred out and used to blank measurements. Set BlankVolume to Null to indicate blanks should be read inside their current containers.
    Default Value: Automatic
    Default Calculation: If BlankMeasurement is True, automatically set to the value of AssayVolume if that was specified, or maximum volume of the container otherwise.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 4000 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 4000*Microliter]) | Automatic) | Null
    Index Matches to: Sample

    BlankAbsorbance

    For each members of SamplesIn, indicates if a corresponding blank measurement, which consists of a separate container (well, chamber, or cuvette) than SampleLink, will be performed, prior to measuring the absorbance of samples. If using Cuvettes, the BlankAbsorbance will be read at the same time as the SampleLink.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Sample Handling

    PlateReaderMixSchedule

    Indicates the points at which mixing should occur in the plate reader, for instance after every set of injections.
    Default Value: Automatic
    Default Calculation: Automatically set to AfterInjections when other mix options are set and injections are specified or to BetweenReadings when mixing is specified but there are no injections.
    Pattern Description: BeforeReadings, BetweenReadings, or AfterInjections or Null.
    Programmatic Pattern: (MixingScheduleP | Automatic) | Null

    Temperature

    Indicates the temperature the samples will held at prior to measuring absorbance and during data acquisition within the instrument.
    Default Value: Automatic
    Default Calculation: Sets to Ambient if an instrument is capable of manipulating the temperature of the samples.
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: (RangeP[$AmbientTemperature, 45*Celsius] | Ambient) | Automatic

    EquilibrationTime

    The length of time for which the samples equilibrate at the requested temperature before being read.
    Default Value: Automatic
    Default Calculation: If an instrument is capable setting the temperature of the samples, sets to 0 second when Temperature is set to Ambient. Otherwise, it is set to 5 minutes.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    MoatSize

    Indicates the number of concentric perimeters of wells to fill with MoatBuffer in order to slow evaporation of inner assay samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume to add to to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 300*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the sample to use to fill each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover. When using the Cuvette Method, automatically set to True.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    TertiaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the third round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if tertiary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    QuaternaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the fourth round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if quaternary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    Absorbance Measurement

    NumberOfReadings

    Number of redundant readings taken by the detector to determine a single averaged absorbance reading.
    Default Value: Automatic
    Default Calculation: If an instrument capable of adjusting NumberOfReadings is selected, resolves to 100. Otherwise resolves to Null
    Pattern Description: Greater than or equal to 1 and less than or equal to 200 or Null.
    Programmatic Pattern: (RangeP[1, 200] | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument will follow as it measures absorbance in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Automatic
    Default Calculation: Resolves to Row if a plate reader capable of adjusting read direction is selected.
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn or Null.
    Programmatic Pattern: (ReadDirectionP | Automatic) | Null

    RunTime

    The length of time for which absorbance measurements are made.
    Default Value: 45 minutes
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 24 hours.
    Programmatic Pattern: RangeP[1*Minute, 24*Hour]

    ReadOrder

    Indicates if all measurements and injections are done for one well before advancing to the next (Serial) or in cycles in which each well is read once per cycle (Parallel).
    Default Value: Parallel
    Pattern Description: Serial or Parallel.
    Programmatic Pattern: ReadOrderP

    Wavelength

    The wavelength(s) at which absorbance is measured. All indicates that the full spectrum available to the plate reader is used.
    Default Value: Automatic
    Default Calculation: Automatically resolves to the wavelengths specified in the input samples' ExtinctionCoefficients field or 260 Nanometer if that field is not populated, for Serial ReadOrder or Matrix SamplingPattern. Otherwise automatically resolves to All.
    Pattern Description: All or a span from anything greater than or equal to 220 nanometers and less than or equal to 1000 nanometers to anything greater than or equal to 220 nanometers and less than or equal to 1000 nanometers or greater than or equal to 220 nanometers and less than or equal to 1000 nanometers or list of one or more greater than or equal to 220 nanometers and less than or equal to 1000 nanometers entries.
    Programmatic Pattern: (All | RangeP[220*Nanometer, 1000*Nanometer] | {RangeP[220*Nanometer, 1000*Nanometer]..} | RangeP[220*Nanometer, 1000*Nanometer] ;; RangeP[220*Nanometer, 1000*Nanometer]) | Automatic

    Sampling

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Default Calculation: When using the BMG plate readers, automatically set to Matrix if SamplingDimension is specified or Ring if SamplingDistance is specified. Otherwise set to Center.
    Pattern Description: Matrix, Spiral, Ring, or Center or Null.
    Programmatic Pattern: (PlateReaderSamplingP | Automatic) | Null

LuminescenceSpectroscopy

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    Sample Handling

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during luminescence measurement. Set the corresponding times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume. times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    Temperature

    The temperature at which the plate reader chamber should be held.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the requested temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument should follow as it measures luminescence in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Default Calculation: Defaults to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to the min volume of the assay plate if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    Instrument

    The plate reader used to measure luminescence intensity.
    Figure 3.11: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: The first plate reader model capable of providing the requested wavelengths is used.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    IntegrationTime

    The amount of time over which luminescence measurements should be integrated. Select a higher time to increase the reading intensity.
    Default Value: 1 second
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 100 seconds or Null.
    Programmatic Pattern: RangeP[0.01*Second, 100*Second] | Null

    ReadLocation

    Indicates if luminescence is measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    EmissionWavelengthRange

    Defines the wavelengths at which luminescence emitted from the sample should be measured.
    Default Value: 350 nanometers ;; 740 nanometers
    Default Calculation: Uses the entire wavelength range available to the specified plate reader.
    Pattern Description: A span from anything greater than or equal to 350 nanometers and less than or equal to 740 nanometers to anything greater than or equal to 350 nanometers and less than or equal to 740 nanometers.
    Programmatic Pattern: RangeP[350*Nanometer, 740*Nanometer] ;; RangeP[350*Nanometer, 740*Nanometer]

    AdjustmentEmissionWavelength

    The wavelength at which luminescence should be read in order to perform automatic adjustments of gain and focal height values.
    Default Value: Automatic
    Default Calculation: Uses the wavelength in the middle of the requested emission wavelength range if the gain is not being set directly.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    Gain

    The gain which should be applied to the signal reaching the primary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample intensity is at the specified percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: Defaults to 90% if AdjustmentSamples is set or if using Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"] which can scan the entire plate and thus don't require an AdjustmentSample to set the gain. Otherwise defaults to 2500 Microvolt.
    Pattern Description: Greater than or equal to 0 microvolts and less than or equal to 4095 microvolts or greater than or equal to 0 percent and less than or equal to 95 percent.
    Programmatic Pattern: (RangeP[0*Percent, 95*Percent] | RangeP[0*Microvolt, 4095*Microvolt]) | Automatic

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Default Calculation: Defaults to FullPlate when using an instrument capable of scanning the full plate during gain adjustments (Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"]). Otherwise the sample with the highest concentration will be used.
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point where light from the sample is directed onto the detector. If set to Auto the height which corresponds to the highest AdjustmentSample signal will be used.
    Default Value: Automatic
    Default Calculation: If an adjustment sample is provided the height which corresponds to the highest AdjustmentSample signal will be used (as indicated by Auto). Otherwise defaults to 7 Millimeter.
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

LuminescenceIntensity

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    Sample Handling

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during luminescence measurement. Set the corresponding times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume. times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    Temperature

    The temperature at which the plate reader chamber should be held.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the requested temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument should follow as it measures luminescence in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Default Calculation: Defaults to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to the min volume of the assay plate if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    WavelengthSelection

    Indicates if the emission wavelengths should be obtained by filters or monochromators.
    Default Value: Automatic
    Default Calculation: Resolves to use filters provided all of the requested wavelengths can be achieved with the current set of filters.
    Pattern Description: NoFilter, Filters, or Monochromators.
    Programmatic Pattern: LuminescenceWavelengthSelectionP | Automatic

    EmissionWavelength

    The wavelength(s) at which luminescence emitted from the sample should be measured.
    Default Value: Automatic
    Default Calculation: Defaults to NoFilter unless DualEmissionWavelengths are specified.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or NoFilter or Null.
    Programmatic Pattern: ((RangeP[320*Nanometer, 740*Nanometer] | NoFilter) | Automatic) | Null
    Index Matches to: EmissionWavelength

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Default Calculation: Defaults to FullPlate when using an instrument capable of scanning the full plate during gain adjustments (Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"]). Otherwise the sample with the highest concentration will be used.
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null
    Index Matches to: EmissionWavelength

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point where light from the sample is directed onto the detector. If set to Auto the height which corresponds to the highest AdjustmentSample signal will be used.
    Default Value: Automatic
    Default Calculation: If an adjustment sample is provided the height which corresponds to the highest AdjustmentSample signal will be used (as indicated by Auto). Otherwise defaults to 7 Millimeter.
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null
    Index Matches to: EmissionWavelength

    Gain

    The gain which should be applied to the signal reaching the primary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample intensity is at the specified percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: Defaults to 90% if AdjustmentSamples is set or if using Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"] which can scan the entire plate and thus don't require an AdjustmentSample to set the gain. Otherwise defaults to 2500 Microvolt.
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent.
    Programmatic Pattern: (RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic
    Index Matches to: EmissionWavelength

    DualEmissionWavelength

    The wavelength at which luminescence emitted from the sample should be measured with the secondary detector (simultaneous to the measurement at the emission wavelength done by the primary detector).
    Default Value: Automatic
    Default Calculation: Uses the second emission wavelength provided by the optic module being used.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null
    Index Matches to: EmissionWavelength

    DualEmissionGain

    The gain which should be applied to the signal reaching the secondary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample intensity is at the specified percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If dual emission readings are requested defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt.
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic) | Null
    Index Matches to: EmissionWavelength

    Instrument

    The plate reader used to measure luminescence intensity.
    Figure 3.12: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: The first plate reader model capable of providing the requested wavelengths is used.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    IntegrationTime

    The amount of time over which luminescence measurements should be integrated. Select a higher time to increase the reading intensity.
    Default Value: 1 second
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 100 seconds or Null.
    Programmatic Pattern: RangeP[0.01*Second, 100*Second] | Null

    ReadLocation

    Indicates if luminescence is measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

LuminescenceKinetics

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    Sample Handling

    TertiaryInjectionSample

    The sample to be injected in the third round of injections. Note that only two unique samples can be injected in total. The corresponding injection times and volumes can be set with TertiaryInjectionTime and TertiaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    QuaternaryInjectionSample

    The sample to be injected in the fourth round of injections. Note that only two unique samples can be injected in total. The corresponding injection times and volumes can be set with QuaternaryInjectionTime and QuaternaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    TertiaryInjectionVolume

    The amount of the tertiary sample which should be injected in the third round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    QuaternaryInjectionVolume

    The amount of the quaternary sample which should be injected in the fourth round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during luminescence measurement. Set the corresponding times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume. times and volumes with PrimaryInjectionTime and PrimaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections. Set the corresponding injection times and volumes with SecondaryInjectionTime and SecondaryInjectionVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    PlateReaderMixSchedule

    Indicates the points at which mixing should occur in the plate reader, for instance after every set of injections.
    Default Value: Automatic
    Default Calculation: When other mix options are set defaults to AfterInjections when injections are specified or BetweenReadings otherwise.
    Pattern Description: BeforeReadings, BetweenReadings, or AfterInjections or Null.
    Programmatic Pattern: (MixingScheduleP | Automatic) | Null

    PrimaryInjectionTime

    The time at which the first round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    SecondaryInjectionTime

    The time at which the second round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    TertiaryInjectionTime

    The time at which the third round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    QuaternaryInjectionTime

    The time at which the fourth round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    TertiaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the third round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if tertiary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    QuaternaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the fourth round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if quaternary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    Temperature

    The temperature at which the plate reader chamber should be held.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the requested temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicate the plate path the instrument should follow as it measures luminescence in each well, for instance reading all wells in a row before continuing on to the next row (Row).
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Default Calculation: Defaults to 1 if any other moat options are specified.
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to the min volume of the assay plate if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Default Calculation: Defaults to Milli-Q water if any other moat options are specified.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    RunTime

    The length of time over which luminescence measurements should be made.
    Default Value: 45 minutes
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 24 hours.
    Programmatic Pattern: RangeP[1*Minute, 24*Hour]

    ReadOrder

    Indicates if all measurements and injections should be done for one well before advancing to the next (serial) or in cycles in which each well is read once per cycle (parallel).
    Default Value: Parallel
    Pattern Description: Serial or Parallel.
    Programmatic Pattern: ReadOrderP

    WavelengthSelection

    Indicates if the emission wavelengths should be obtained by filters or monochromators.
    Default Value: Automatic
    Default Calculation: Resolves to use filters provided all of the requested wavelengths can be achieved with the current set of filters.
    Pattern Description: NoFilter, Filters, or Monochromators.
    Programmatic Pattern: LuminescenceWavelengthSelectionP | Automatic

    EmissionWavelength

    The wavelength(s) at which luminescence emitted from the sample should be measured.
    Default Value: Automatic
    Default Calculation: Defaults to NoFilter unless DualEmissionWavelengths are specified.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or NoFilter or Null.
    Programmatic Pattern: ((RangeP[320*Nanometer, 740*Nanometer] | NoFilter) | Automatic) | Null
    Index Matches to: EmissionWavelength

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Default Calculation: Defaults to FullPlate when using an instrument capable of scanning the full plate during gain adjustments (Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"]). Otherwise the sample with the highest concentration will be used.
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null
    Index Matches to: EmissionWavelength

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point where light from the sample is directed onto the detector. If set to Auto the height which corresponds to the highest AdjustmentSample signal will be used.
    Default Value: Automatic
    Default Calculation: If an adjustment sample is provided the height which corresponds to the highest AdjustmentSample signal will be used (as indicated by Auto). Otherwise defaults to 7 Millimeter.
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null
    Index Matches to: EmissionWavelength

    Gain

    The gain which should be applied to the signal reaching the primary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample intensity is at the specified percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: Defaults to 90% if AdjustmentSamples is set or if using Model[Instrument,PlateReader,"CLARIOstar"] or Model[Instrument,PlateReader,"PHERAstar FS"] which can scan the entire plate and thus don't require an AdjustmentSample to set the gain. Otherwise defaults to 2500 Microvolt.
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent.
    Programmatic Pattern: (RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic
    Index Matches to: EmissionWavelength

    DualEmissionWavelength

    The wavelength at which luminescence emitted from the sample should be measured with the secondary detector (simultaneous to the measurement at the emission wavelength done by the primary detector).
    Default Value: Automatic
    Default Calculation: Uses the second emission wavelength provided by the optic module being used.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null
    Index Matches to: EmissionWavelength

    DualEmissionGain

    The gain which should be applied to the signal reaching the secondary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample intensity is at the specified percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If dual emission readings are requested defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt.
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic) | Null
    Index Matches to: EmissionWavelength

    Instrument

    The plate reader used to measure luminescence intensity.
    Figure 3.13: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: The first plate reader model capable of providing the requested wavelengths is used.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    IntegrationTime

    The amount of time over which luminescence measurements should be integrated. Select a higher time to increase the reading intensity.
    Default Value: 1 second
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 100 seconds or Null.
    Programmatic Pattern: RangeP[0.01*Second, 100*Second] | Null

    ReadLocation

    Indicates if luminescence is measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

FluorescenceSpectroscopy

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    ReadLocation

    Indicates if fluorescence should be measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    Temperature

    The temperature at which the experiment will be performed, if using a plate reader with temperature incubation controls.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the assay temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    NumberOfReadings

    Number of redundant readings which should be taken by the detector to determine a single averaged fluorescence intensity reading.
    Default Value: 100
    Pattern Description: Greater than or equal to 1 and less than or equal to 200.
    Programmatic Pattern: RangeP[1, 200]

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    ExcitationScanGain

    The gain which should be applied to the signal reaching the primary detector during the excitation scan. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample fluoresces at that percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If unspecified defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt
    Pattern Description: Greater than or equal to 0 microvolts and less than or equal to 4095 microvolts or greater than or equal to 0 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[0*Percent, 95*Percent] | RangeP[0*Microvolt, 4095*Microvolt]) | Automatic) | Null

    EmissionScanGain

    The gain which should be applied to the signal reaching the primary detector during the emission scan. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample fluoresces at that percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If unspecified defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt
    Pattern Description: Greater than or equal to 0 microvolts and less than or equal to 4095 microvolts or greater than or equal to 0 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[0*Percent, 95*Percent] | RangeP[0*Microvolt, 4095*Microvolt]) | Automatic) | Null

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point. If set to Automatic, the focal height will be adjusted based on the Gain adjustment, which will occur only if the gain values are set to percentages.
    Default Value: Automatic
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null

    Sample Handling

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during fluorescence measurement.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicates the order in which wells should be read by specifying the plate path the instrument should follow when measuring fluorescence.
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    Instrument

    The plate reader used to measure fluorescence intensity.
    Figure 3.14: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    SpectralScan

    Indicates if fluorescence should be recorded using a fixed excitation wavelength and a range of emission wavelengths (Emission) or using a fixed emission wavelength and a range of excitation wavelengths (Excitation). Specify Emission and Excitation to record both spectra.
    Default Value: Automatic
    Default Calculation: If unspecified uses the emission spectrum if EmissionWavelengthRange is specified and/or the excitation spectrum if ExcitationWavelengthRange is specified, defaulting to measure both spectrum if all options are left Automatic.
    Pattern Description: Excitation or Emission or list of one or more Excitation or Emission entries.
    Programmatic Pattern: ({FluorescenceScanTypeP..} | FluorescenceScanTypeP) | Automatic

    ExcitationWavelength

    The wavelength which should be used to excite fluorescence in the samples. Fluorescence will then be measured over 'EmissionWavelengthRange'.
    Default Value: Automatic
    Default Calculation: If unspecified uses the wavelength 50 Nanometer below the smallest requested emission wavelength.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    EmissionWavelength

    The wavelength at which fluorescence emitted from the sample should be measured after the sample has been excited by each wavelength in 'ExcitationWavelengthRange'.
    Default Value: Automatic
    Default Calculation: If unspecified uses the wavelength 50 Nanometer above the largest requested excitation wavelength.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    ExcitationWavelengthRange

    Defines the wavelengths which should be used to excite fluorescence in the samples. Fluorescence will then be measured at 'EmissionWavelength'.
    Default Value: Automatic
    Default Calculation: If unspecified uses the largest possible range of excitation wavelengths with the constraints that the range must be within the instrument operating limits and below the emission wavelength.
    Pattern Description: A span from anything greater than or equal to 320 nanometers and less than or equal to 740 nanometers to anything greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] ;; RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    EmissionWavelengthRange

    Defines the wavelengths at which fluorescence emitted from the sample should be measured after the sample has been excited by 'ExcitationWavelength'.
    Default Value: Automatic
    Default Calculation: If unspecified uses the largest possible range of emission wavelengths with the constraints that the range must be within the instrument operating limits and above the excitation wavelength.
    Pattern Description: A span from anything greater than or equal to 320 nanometers and less than or equal to 740 nanometers to anything greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] ;; RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

    Spectral Scanning

    AdjustmentEmissionWavelength

    The wavelength at which fluorescence should be read in order to perform automatic adjustments of gain and focal height values at run time.
    Default Value: Automatic
    Default Calculation: If unspecified uses the wavelength in the middle of the requested emission wavelength range if the gain is not being set directly.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

    AdjustmentExcitationWavelength

    The wavelength at which the sample should be excited in order to perform automatic adjustments of gain and focal height values at run time.
    Default Value: Automatic
    Default Calculation: If unspecified uses the wavelength in the middle of the requested excitation wavelength range if the gain is not being set directly.
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null

FluorescenceIntensity

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    Mode

    The type of fluorescence reading which should be performed.
    Default Value: Automatic
    Pattern Description: Fluorescence or TimeResolvedFluorescence.
    Programmatic Pattern: (Fluorescence | TimeResolvedFluorescence) | Automatic

    WavelengthSelection

    Indicates if the emission and excitation wavelengths should be obtained by filters or monochromators.
    Default Value: Automatic
    Pattern Description: Filters or Monochromators.
    Programmatic Pattern: WavelengthSelectionP | Automatic

    Gain

    The gain which should be applied to the signal reaching the primary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample fluoresces at that percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If unspecified defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent.
    Programmatic Pattern: (RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic
    Index Matches to: ExcitationWavelength

    DualEmissionGain

    The gain to apply to the signal reaching the secondary detector. This may be specified either as a direct voltage, or as a percentage relative to the AdjustmentSample option.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point. If set to Automatic, the focal height will be adjusted based on the Gain adjustment, which will occur only if the gain values are set to percentages.
    Default Value: Automatic
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    DelayTime

    The amount of time which should be allowed to pass after excitation and before fluorescence measurement begins.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 microseconds and less than or equal to 8000 microseconds or Null.
    Programmatic Pattern: (RangeP[0*Microsecond, 8000*Microsecond] | Automatic) | Null

    ReadTime

    The amount of time for which the fluorescence measurement reading should occur.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 microsecond and less than or equal to 10000 microseconds or Null.
    Programmatic Pattern: (RangeP[1*Microsecond, 10000*Microsecond] | Automatic) | Null

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    ReadLocation

    Indicates if fluorescence should be measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    Temperature

    The temperature at which the experiment will be performed, if using a plate reader with temperature incubation controls.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the assay temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    NumberOfReadings

    Number of redundant readings which should be taken by the detector to determine a single averaged fluorescence intensity reading.
    Default Value: 100
    Pattern Description: Greater than or equal to 1 and less than or equal to 200.
    Programmatic Pattern: RangeP[1, 200]

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    Sample Handling

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during fluorescence measurement.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicates the order in which wells should be read by specifying the plate path the instrument should follow when measuring fluorescence.
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    ExcitationWavelength

    The wavelength(s) which should be used to excite fluorescence in the samples.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers.
    Programmatic Pattern: RangeP[320*Nanometer, 740*Nanometer] | Automatic
    Index Matches to: ExcitationWavelength

    EmissionWavelength

    The wavelength(s) at which fluorescence emitted from the sample should be measured.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers.
    Programmatic Pattern: RangeP[320*Nanometer, 740*Nanometer] | Automatic
    Index Matches to: ExcitationWavelength

    DualEmissionWavelength

    The wavelength at which fluorescence emitted from the sample should be measured with the secondary detector (simultaneous to the measurement at the emission wavelength done by the primary detector).
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null
    Index Matches to: ExcitationWavelength

    Instrument

    The plate reader used to measure fluorescence intensity.
    Figure 3.15: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

FluorescenceKinetics

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    RunTime

    The length of time over which fluorescence measurements should be made.
    Default Value: 45 minutes
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 24 hours.
    Programmatic Pattern: RangeP[1*Minute, 24*Hour]

    ReadOrder

    Indicates if all measurements and injections should be done for one well before advancing to the next (serial) or in cycles in which each well is read once per cycle (parallel).
    Default Value: Parallel
    Pattern Description: Serial or Parallel.
    Programmatic Pattern: ReadOrderP

    PlateReaderMixSchedule

    Indicates the points at which mixing should occur in the plate reader.
    Default Value: Automatic
    Pattern Description: BeforeReadings, BetweenReadings, or AfterInjections or Null.
    Programmatic Pattern: (MixingScheduleP | Automatic) | Null

    Mode

    The type of fluorescence reading which should be performed.
    Default Value: Automatic
    Pattern Description: Fluorescence or TimeResolvedFluorescence.
    Programmatic Pattern: (Fluorescence | TimeResolvedFluorescence) | Automatic

    WavelengthSelection

    Indicates if the emission and excitation wavelengths should be obtained by filters or monochromators.
    Default Value: Automatic
    Pattern Description: Filters or Monochromators.
    Programmatic Pattern: WavelengthSelectionP | Automatic

    Gain

    The gain which should be applied to the signal reaching the primary detector. This may be specified either as a direct voltage, or as a percentage (which indicates that the gain should be set such that the AdjustmentSample fluoresces at that percentage of the instrument's dynamic range).
    Default Value: Automatic
    Default Calculation: If unspecified defaults to 90% if an adjustment sample is provided or if the instrument can scan the entire plate to determine gain. Otherwise defaults to 2500 Microvolt
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent.
    Programmatic Pattern: (RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic
    Index Matches to: ExcitationWavelength

    DualEmissionGain

    The gain to apply to the signal reaching the secondary detector. This may be specified either as a direct voltage, or as a percentage relative to the AdjustmentSample option.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or greater than or equal to 1 percent and less than or equal to 95 percent or Null.
    Programmatic Pattern: ((RangeP[1*Percent, 95*Percent] | RangeP[1*Microvolt, 4095*Microvolt]) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    AdjustmentSample

    The sample which should be used to perform automatic adjustments of gain and/or focal height values at run time. The focal height will be set so that the highest signal-to-noise ratio can be achieved for the AdjustmentSample. The gain will be set such that the AdjustmentSample fluoresces at the specified percentage of the instrument's dynamic range. When multiple aliquots of the same sample is used in the experiment, an index can be specified to use the desired aliquot for adjustments. When set to FullPlate, all wells of the assay plate are scanned and the well of the highest fluorescence intensity if perform gain and focal height adjustments.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or FullPlate or {Index, Sample} or Null.
    Programmatic Pattern: ((FullPlate | (ObjectP[Object[Sample]] | _String) | {RangeP[1, 384, 1], ObjectP[Object[Sample]] | _String}) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    FocalHeight

    The distance from the bottom of the plate carrier to the focal point. If set to Automatic, the focal height will be adjusted based on the Gain adjustment, which will occur only if the gain values are set to percentages.
    Default Value: Automatic
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: ((RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic) | Null
    Index Matches to: ExcitationWavelength

    DelayTime

    The amount of time which should be allowed to pass after excitation and before fluorescence measurement begins.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 microseconds and less than or equal to 8000 microseconds or Null.
    Programmatic Pattern: (RangeP[0*Microsecond, 8000*Microsecond] | Automatic) | Null

    ReadTime

    The amount of time for which the fluorescence measurement reading should occur.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 microsecond and less than or equal to 10000 microseconds or Null.
    Programmatic Pattern: (RangeP[1*Microsecond, 10000*Microsecond] | Automatic) | Null

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. When this option is set to True, injections cannot be performed as it's not possible to inject samples through the cover.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    ReadLocation

    Indicates if fluorescence should be measured using an optic above the plate or one below the plate.
    Default Value: Automatic
    Default Calculation: Defaults to Bottom if RetainCover is set to True, otherwise defaults to Top.
    Pattern Description: Top or Bottom.
    Programmatic Pattern: ReadLocationP | Automatic

    Temperature

    The temperature at which the experiment will be performed, if using a plate reader with temperature incubation controls.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    EquilibrationTime

    The length of time for which the assay plates should equilibrate at the assay temperature in the plate reader before being read.
    Default Value: Automatic
    Default Calculation: Defaults to 0 Second if Temperature is set to Ambient. Otherwise defaults to 5 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 1 hour.
    Programmatic Pattern: RangeP[0*Minute, 1*Hour] | Automatic

    NumberOfReadings

    Number of redundant readings which should be taken by the detector to determine a single averaged fluorescence intensity reading.
    Default Value: 100
    Pattern Description: Greater than or equal to 1 and less than or equal to 200.
    Programmatic Pattern: RangeP[1, 200]

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    NumberOfReplicates

    Number of times each of the input samples should be analyzed using identical experimental parameters.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    PrimaryInjectionTime

    The time at which the first round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    SecondaryInjectionTime

    The time at which the second round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    TertiaryInjectionTime

    The time at which the third round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    QuaternaryInjectionTime

    The time at which the fourth round of injections should start.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 seconds or Null.
    Programmatic Pattern: GreaterEqualP[0*Second] | Null

    Sample Handling

    PrimaryInjectionSample

    The sample to be injected in the first round of injections in order to introduce a time sensitive reagent/sample into the plate before/during fluorescence measurement.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    SecondaryInjectionSample

    The sample to be injected in the second round of injections.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    PrimaryInjectionVolume

    The amount of the primary sample which should be injected in the first round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    SecondaryInjectionVolume

    The amount of the secondary sample which should be injected in the second round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    TertiaryInjectionSample

    The sample to be injected in the third round of injections.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    QuaternaryInjectionSample

    The sample to be injected in the fourth round of injections.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    TertiaryInjectionVolume

    The amount of the tertiary sample which should be injected in the third round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    QuaternaryInjectionVolume

    The amount of the quaternary sample which should be injected in the fourth round of injections.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (Null | RangeP[0.5*Microliter, 300*Microliter]) | Null
    Index Matches to: Sample

    MoatSize

    Indicates the number of concentric perimeters of wells which should be should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Pattern Description: Greater than 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the RecommendedFillVolume of the assay plate if informed, or 75% of the MaxVolume of the assay plate if not, if any other moat options are specified.
    Pattern Description: Greater than 0 microliters or Null.
    Programmatic Pattern: (GreaterP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    PrimaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the first round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if primary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    SecondaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the second round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if secondary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate should be agitated inside the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    ReadDirection

    Indicates the order in which wells should be read by specifying the plate path the instrument should follow when measuring fluorescence.
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    InjectionSampleStorageCondition

    The non-default conditions under which any injection samples used by this experiment should be stored after the protocol is completed.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (SampleStorageTypeP | Disposal) | Null

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    TertiaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the third round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if tertiary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    QuaternaryInjectionFlowRate

    The speed at which to transfer injection samples into the assay plate in the fourth round of injections.
    Default Value: Automatic
    Default Calculation: Defaults to 300 Microliter/Second if quaternary injections are specified.
    Pattern Description: 430 microliters per second, 400 microliters per second, 350 microliters per second, 300 microliters per second, 260 microliters per second, 220 microliters per second, 190 microliters per second, 170 microliters per second, 150 microliters per second, 135 microliters per second, 115 microliters per second, 100 microliters per second, 85 microliters per second, 65 microliters per second, or 50 microliters per second or Null.
    Programmatic Pattern: (BMGFlowRateP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    Optics

    ExcitationWavelength

    The wavelength(s) which should be used to excite fluorescence in the samples.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers.
    Programmatic Pattern: RangeP[320*Nanometer, 740*Nanometer] | Automatic
    Index Matches to: ExcitationWavelength

    EmissionWavelength

    The wavelength(s) at which fluorescence emitted from the sample should be measured.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers.
    Programmatic Pattern: RangeP[320*Nanometer, 740*Nanometer] | Automatic
    Index Matches to: ExcitationWavelength

    DualEmissionWavelength

    The wavelength at which fluorescence emitted from the sample should be measured with the secondary detector (simultaneous to the measurement at the emission wavelength done by the primary detector).
    Default Value: Automatic
    Pattern Description: Greater than or equal to 320 nanometers and less than or equal to 740 nanometers or Null.
    Programmatic Pattern: (RangeP[320*Nanometer, 740*Nanometer] | Automatic) | Null
    Index Matches to: ExcitationWavelength

    Instrument

    The plate reader used to measure fluorescence intensity.
    Figure 3.16: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    Sampling

    SamplingPattern

    Indicates where in the well measurements are taken.
    Default Value: Automatic
    Pattern Description: Matrix, Spiral, Ring, or Center.
    Programmatic Pattern: PlateReaderSamplingP | Automatic

    SamplingDistance

    Indicates the length of the region over which sampling measurements are taken.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null if SamplingPattern is set to Center otherwise resolves to 80% of the diameter of the well.
    Pattern Description: Greater than or equal to 1 millimeter and less than or equal to 6 millimeters or Null.
    Programmatic Pattern: (RangeP[1*Millimeter, 6*Millimeter] | Automatic) | Null

    SamplingDimension

    Specifies the size of the grid used for Matrix sampling. For example SamplingDimension->5 will scan a 5 x 5 grid.
    Default Value: Automatic
    Default Calculation: If the SamplingPattern is set to Matrix, SamplingDimension will be set to 3.
    Pattern Description: Greater than or equal to 2 and less than or equal to 30 or Null.
    Programmatic Pattern: (RangeP[2, 30] | Automatic) | Null

AlphaScreen

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that will be measured by the plate reader instrument.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples for which alpha screen measurements are taken, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers holding the samples for which alpha screen measurements are taken, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    PreparedPlate

    Indicates if a prepared plate is provided for AlphaScreen measurement. The prepared plate contains the analytes, acceptor and donor AlphaScreen beads that are ready to be excited by AlphaScreen laser for luminescent AlphaScreen measurement in a plate reader. If the 'PreparedPlate' is False, the samples that contain all the components will be transferred to an assay plate for AlphaScreen measurement.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Instrument

    The plate reader for the signal measurement in AlphaScreen.
    Figure 3.17: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}] | Automatic

    AssayPlateModel

    The plate where the samples are transferred and measured in the plate reader.
    Default Value: Automatic
    Default Calculation: Automatic resolves the plate where the 'AlphaAssayVolume' is within the volume limits. If multiple plate formats support the 'AlphaAssayVolume', automatic resolves to a half area 96-well plate if the number of samples is less than 96, or resolves to a 384-well plate if the number of samples is more than 96. If the 'AlphaAssayVolume' is also set to Automatic, automatic resolves to either a half are 96-well or 384-well plate based on the number of samples as described before.
    Pattern Description: An object of type or subtype Model[Container, Plate] or Null.
    Programmatic Pattern: (ObjectP[{Model[Container, Plate]}] | Automatic) | Null

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    StoreMeasuredPlates

    Indicate if the assay plates are stored after the experiment is completed. If it sets to True, the plates are stored according to the storage condition of the samples after measurement. If it sets to False, the plates are discarded after measurement.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

    AlphaScreen Sample Preparation

    AlphaAssayVolume

    The total volume that each sample is transferred to the assay plate for luminescent AlphaScreen measurement.
    Default Value: Automatic
    Default Calculation: Automatic resolves to the recommended fill volume of the 'AssayPlateModel'. If the 'AssayPlateModel' is also set to Automatic, automatic resolves to 100 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (RangeP[0*Microliter, 300*Microliter] | Automatic) | Null

    NumberOfReplicates

    The number of wells each sample is aliquoted into and read independently.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 and less than or equal to 384 in increments of 1 or Null.
    Programmatic Pattern: RangeP[0, 384, 1] | Null

    Optics

    ReadTemperature

    The temperature at which the measurement will be taken in the plate reader.
    Default Value: 37 degrees Celsius
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    ReadEquilibrationTime

    The length of time for which the assay plates should equilibrate at the assay temperature in the plate reader before being read.
    Default Value: 2 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 2 hours.
    Programmatic Pattern: RangeP[0*Minute, 2*Hour]

    TargetCarbonDioxideLevel

    The target amount of carbon dioxide in the atmosphere in the plate reader chamber.
    Default Value: Automatic
    Default Calculation: Automatically set to 5% for mammalian cells, and Null otherwise.
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: (RangeP[0.1*Percent, 20*Percent] | Automatic) | Null

    TargetOxygenLevel

    The target amount of oxygen in the atmosphere in the plate reader chamber. If specified, nitrogen gas is pumped into the chamber to force oxygen in ambient air out of the chamber until the desired level is reached.
    Default Value: Null
    Pattern Description: Greater than or equal to 0.1 percent and less than or equal to 20 percent or Null.
    Programmatic Pattern: RangeP[0.1*Percent, 20*Percent] | Null

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: Automatic or True or False.
    Programmatic Pattern: (BooleanP | Automatic) | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate is agitated inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    Gain

    The gain which should be applied to the signal reaching the primary detector photomultiplier tube (PMT). This is specified as a direct voltage.
    Default Value: Automatic
    Default Calculation: Automatic set to 3600 Microvolt.
    Pattern Description: Automatic or greater than or equal to 1 microvolt and less than or equal to 4095 microvolts.
    Programmatic Pattern: (RangeP[1*Microvolt, 4095*Microvolt] | Automatic) | Automatic

    FocalHeight

    The distance from the focal point to the lens that collect the light to the primary detector photomultiplier tube (PMT).
    Default Value: Automatic
    Default Calculation: Automatic set to Auto, which indicates that the focal height will be selected by the instrument.
    Pattern Description: Auto or greater than or equal to 0 millimeters and less than or equal to 25 millimeters.
    Programmatic Pattern: (RangeP[0*Millimeter, 25*Millimeter] | Auto) | Automatic

    SettlingTime

    The time between the movement of the plate and the beginning of the measurement. It reduces potential vibration of the samples in plate due to the stop and go motion of the plate carrier.
    Default Value: 0 milliseconds
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 1 second.
    Programmatic Pattern: RangeP[0*Second, 1*Second]

    ExcitationTime

    The time that the samples will be excited by the light source and the singlet Oxygen is generated.
    Default Value: 80 milliseconds
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 1 second.
    Programmatic Pattern: RangeP[0.01*Second, 1*Second]

    DelayTime

    The time between end of excitation and start of the emission signal integration. It allows the singlet Oxygen to react with the acceptor beads and reduces potential auto-fluorescent noise generated by biomolecular components.
    Default Value: 120 milliseconds
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 2 seconds.
    Programmatic Pattern: RangeP[0*Second, 2*Second]

    IntegrationTime

    The amount of time for which the emission signal is integrated.
    Default Value: 300 milliseconds
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 2 seconds.
    Programmatic Pattern: RangeP[0.01*Second, 2*Second]

    ExcitationWavelength

    The excitation wavelength determined by the AlphaScreen laser.
    Default Value: 680 nanometers
    Pattern Description: Greater than or equal to 300 nanometers and less than or equal to 700 nanometers in increments of 1 nanometer.
    Programmatic Pattern: RangeP[300*Nanometer, 700*Nanometer, 1*Nanometer]

    EmissionWavelength

    The emission wavelength determined by the optical filter.
    Default Value: 570 nanometers
    Pattern Description: 570 nanometers, 615 nanometers, 665 nanometers, or 620 nanometers.
    Programmatic Pattern: 570*Nanometer | 615*Nanometer | 665*Nanometer | 620*Nanometer

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. It is strongly recommended not to retain a cover because AlphaScreen can only read from top.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    ReadDirection

    Indicates the order in which wells should be read by specifying the plate path the instrument should follow when measuring signal. Default sets to Row, where the plate is read from left to right for each row.
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    Sample Handling

    AtmosphereEquilibrationTime

    The length of time for which the samples equilibrate at the requested oxygen and carbon dioxide level before being read.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute if TargetCarbonDioxideLevel or TargetOxygenLevel is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 24 hours or Null.
    Programmatic Pattern: (RangeP[0*Second, 24*Hour] | Automatic) | Null

    MoatWells

    Indicates if moat wells are set to decrease evaporation from the assay samples during the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other moat options are specified.
    Pattern Description: Automatic or True or False or Null.
    Programmatic Pattern: ((BooleanP | Automatic) | Automatic) | Null

    MoatSize

    Indicates the number of concentric perimeters of wells which should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any other moat options are specified. Resolves Null if MoatWells is False.
    Pattern Description: Greater than or equal to 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the minimum volume of the assay plate if any other moat options are specified.
    Pattern Description: Greater than or equal to 0 microliters or Null.
    Programmatic Pattern: (GreaterEqualP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if any other moat options are specified.]
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

Pellet

    pelletRules

    Specific key/value pairs specifying the samples/containers to be involved in the pellet primitive.
    Pattern: {Sample -> ListableP[ObjectP[{Object[Sample], Object[Container]}]], Instrument -> ListableP[Automatic | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]], Intensity -> ListableP[Automatic | GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]], Time -> ListableP[Automatic | TimeP], Temperature -> ListableP[Automatic | Ambient | RangeP[4*Celsius, 40*Celsius]], SupernatantDestination -> ListableP[ObjectP[{Object[Sample], Object[Container]}] | Waste], ResuspensionSource -> ListableP[ObjectP[{Object[Sample], Object[Container]}]], Sequence[SupernatantVolume -> Hold[ListableP[(All | RangeP[1*Microliter, 20*Liter]) | Automatic]], SupernatantTransferInstrument -> Hold[ListableP[((Null | (ObjectP[{Model[Container, Syringe], Model[Container, GraduatedCylinder], Model[Instrument, Pipette], Model[Instrument, Aspirator], Model[Item, Spatula], Model[Item, Tweezer], Model[Item, TransferTube], Model[Item, ChippingHammer], Model[Item, Scissors], Object[Container, Syringe], Object[Container, GraduatedCylinder], Object[Instrument, Pipette], Object[Instrument, Aspirator], Object[Item, Spatula], Object[Item, Tweezer], Object[Item, TransferTube], Object[Item, ChippingHammer], Object[Item, Scissors]}] | _String)) | Automatic) | Null]], ResuspensionVolume -> Hold[ListableP[((All | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null]], ResuspensionInstrument -> Hold[ListableP[((Null | (ObjectP[{Model[Container, Syringe], Model[Container, GraduatedCylinder], Model[Instrument, Pipette], Model[Instrument, Aspirator], Model[Item, Spatula], Model[Item, Tweezer], Model[Item, TransferTube], Model[Item, ChippingHammer], Model[Item, Scissors], Object[Container, Syringe], Object[Container, GraduatedCylinder], Object[Instrument, Pipette], Object[Instrument, Aspirator], Object[Item, Spatula], Object[Item, Tweezer], Object[Item, TransferTube], Object[Item, ChippingHammer], Object[Item, Scissors]}] | _String)) | Automatic) | Null]], ResuspensionMix -> Hold[ListableP[((Null | BooleanP) | Automatic) | Null]], ResuspensionMixType -> Hold[ListableP[((Null | MixTypeP) | Automatic) | Null]], ResuspensionMixUntilDissolved -> Hold[ListableP[((Null | BooleanP) | Automatic) | Null]], ResuspensionMixInstrument -> Hold[ListableP[((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null]], ResuspensionMixTime -> Hold[ListableP[((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null]], ResuspensionMixMaxTime -> Hold[ListableP[((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null]], ResuspensionMixDutyCycle -> Hold[ListableP[((Null | {RangeP[0*Minute, 60*Hour], RangeP[0*Minute, 60*Hour]}) | Automatic) | Null]], ResuspensionMixRate -> Hold[ListableP[((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null]], ResuspensionNumberOfMixes -> Hold[ListableP[((Null | RangeP[1, 50, 1]) | Automatic) | Null]], ResuspensionMaxNumberOfMixes -> Hold[ListableP[((Null | RangeP[1, 250, 1]) | Automatic) | Null]], ResuspensionMixVolume -> Hold[ListableP[((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null]], ResuspensionMixTemperature -> Hold[ListableP[((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null]], ResuspensionMixMaxTemperature -> Hold[ListableP[((Null | RangeP[0*Celsius, 100*Celsius]) | Automatic) | Null]], ResuspensionMixAmplitude -> Hold[ListableP[((Null | RangeP[10*Percent, 100*Percent]) | Automatic) | Null]]]}

    General

    Sample

    The samples that should be pelleted.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples whose denser or insoluble contents will be concentrated to the bottom of a given container, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers of the samples whose denser or insoluble contents will be concentrated to the bottom of a given container, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleOutLabel

    A user defined word or phrase used to identify the samples transferred into SupernatantDestinations, for use in downstream unit operations. If SupernatantDestination is set to Waste, SampleOutLabel should be Null as the sample out cannot be labeled for further use.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ContainerOutLabel

    A user defined word or phrase used to identify the containers of the samples transferred into SupernatantDestinations, for use in downstream unit operations. If SupernatantDestination is set to Waste, ContainerOutLabel should be Null as the waste container cannot be labeled for further use.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Centrifugation

    Instrument

    The centrifuge that will be used to spin the provided samples.
    Figure 3.18: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to a centrifuge that can attain the specified intensity, time, temperature, and sterility and (if possible) that is compatible with the sample in its current container.
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge]
    Programmatic Pattern: ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic
    Index Matches to: Sample

    Intensity

    The rotational speed or the force that will be applied to the samples by centrifugation in order to create a pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to one fifth of the maximum rotational rate of the centrifuge, rounded to the nearest attainable centrifuge precision.
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 80000 revolutions per minute or greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 504000 standard accelerations due to gravity on the surface of the earth.
    Programmatic Pattern: (RangeP[0*RPM, $MaxCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRelativeCentrifugalForce]) | Automatic
    Index Matches to: Sample

    Time

    The amount of time that the samples will be centrifuged.
    Default Value: 5 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours.
    Programmatic Pattern: RangeP[0*Minute, $MaxExperimentTime]
    Index Matches to: Sample

    Temperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius.
    Programmatic Pattern: Ambient | RangeP[-10*Celsius, 40*Celsius]
    Index Matches to: Sample

    Supernatant Aspiration

    SupernatantVolume

    The volume of supernatant that will be aspirated from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set based on Preparation: All for Preparation is Manual and half of the sample volume for Preparation is Robotic.
    Pattern Description: All or Volume.
    Programmatic Pattern: (All | RangeP[1*Microliter, 20*Liter]) | Automatic
    Index Matches to: Sample

    SupernatantDestination

    The destination that the supernatant should be dispensed into, after aspirated from the source sample.
    Default Value: Automatic
    Default Calculation: SupernatantDestination is set to Waste if performing the aspiration manually using an aspirator. Otherwise, SupernatantDestination is set to an empty liquid handler container to dispense the supernatant into.
    Pattern Description: Destination Sample/Container or Waste.
    Programmatic Pattern: (Waste | (ObjectP[{Object[Sample], Object[Container], Model[Container]}] | _String)) | Automatic
    Index Matches to: Sample

    Resuspension

    Resuspension

    Indicates if the pellet should be resuspended after the supernatant is aspirated.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any of the other resuspension options are set.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    ResuspensionSource

    The sample that should be used to resuspend the pellet from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if any of the other Resuspension options are set.
    Pattern Description: An object of type or subtype Model[Sample], Object[Sample], or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample], Object[Container]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionSourceLabel

    The label of the samples that should be used to resuspend the pellet from the source sample, which is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionVolume

    The volume of ResuspensionSource that should be used to resuspend the pellet from the source sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/4 of the source sample container's volume or the volume of the provided ResuspensionSource (whichever is smaller), if any of the other Resuspension options are set.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 20*Liter])) | Automatic) | Null
    Index Matches to: Sample

    Resuspension Mix

    ResuspensionMix

    Indicates if this sample is mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any Mix related options are set. Otherwise, set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixType

    Indicates the style of motion used to mix the sample. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically sets based on the container of the sample and the Mix option. Specifically, if Mix is set to False, the option is set to Null. If MixInstrument is specified, the option is set based on the type of the Model[Instrument] or Object[Instrument] of the specified MixInstrument. If MixRate and Time are Null, when MixVolume is Null or larger than 50ml, the option is set to Invert, otherwise set to Pipette. If Amplitude, MaxTemperature, or DutyCycle is not Null, the option is set to Homogenizer. If MixRate is set, the option is set based on the type of the Model[Instrument] of the first instrument found by MixDevices that is compatible with the SamplesIn, Temperature, and MixRate.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixUntilDissolved

    Indicates if the mix should be continued up to the MaxTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixInstrument

    The instrument used to perform the Mix and/or Incubation.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixStirBar

    The stir bar that is used to stir the sample.
    Default Value: Automatic
    Default Calculation: If MixRate is above 1000 RPM, or if no compatible impeller can be found for the given stirrer, StirBar is automatically set to the largest compatible stir bar found. Here, a compatible impeller means that it can fit in the aperture of the sample's container, can reach the bottom of the sample's container, and is compatible with the stir instrument given; a compatible stir bar means that its width can fit in the aperture of the sample's container and that its length is not greater than the InternalDiameter of the sample's container. Otherwise set to Null.
    Pattern Description: An object of type or subtype Model[Part, StirBar] or Object[Part, StirBar] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, StirBar], Object[Part, StirBar]}]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTime

    Duration of time for which the samples will be mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Minute for robotic preparation. For manual preparation, if Thaw is True, set to 0 Minute; else if MaxTime is specified, Time is automatically set to 1/3 of MaxTime; otherwise set to 15 Minute. As a special case in manual preparation, when LightExposure is not Null, Thaw is True, set to 0 Minute; else if TotalLightExposure is specified and LightExposureIntensity is not Null, Time is automatically set to TotalLightExposure/LightExposureIntensity; otherwise set to 1 Hour.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixMaxTime

    Maximum duration of time for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Shake, Roll, Vortex or Sonicate.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 Hour if MixUntilDissolved is True, when an applicable MixType is chosen, otherwise set to Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixDutyCycle

    The style how the homogenizer mixes the given sample via pulsation of the sonication horn. This duty cycle is repeated indefinitely until the specified Time/MaxTime has elapsed. This option can only be set when mixing via homogenization.
    Default Value: Automatic
    Default Calculation: Automatically set to {10 Millisecond, 10 Millisecond} if mixing by homogenization.
    Pattern Description: {Time On, Time Off} or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Minute, 60*Hour], RangeP[0*Minute, 60*Hour]}) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixRate

    The frequency of rotation used by the mixing instrument to mix the samples.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM for robotic preparation. For manual preparation, MixRate is set to the average of MinRotationRate and MaxRotationRate of the instrument.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixRateProfile

    The frequency of rotation of the mixing instrument used to mix the samples, over the course of time.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, MixRate} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration]}..}) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionNumberOfMixes

    The number of times the samples are mixed if MixType of Pipette or Invert is chosen.
    Default Value: Automatic
    Default Calculation: If MaxNumberOfMixes is specified, automatically set to 1/3 of MaxNumberOfMixes (round to integer); else if MixUntilDissolved->True, automatically set to 25; otherwise set to 15.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMaxNumberOfMixes

    The maximum number of times for which the samples are mixed, in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. Note this option only applies for mix type: Pipette or Invert.
    Default Value: Automatic
    Default Calculation: When MixUntilDissolved->True, automatically set to 2*NumberOfMixes if it is Numeric (specified or automatically set); if not, set to 50.
    Pattern Description: Greater than or equal to 1 and less than or equal to 250 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 250, 1]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixVolume

    The volume of the sample that is pipetted up and down to mix if MixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, automatically set to 970 Microliter or sample volume informed by the field Volume of the sample, whichever is smaller. For manual preparation, automatically set to 50 Milliliter or half of the sample volume informed by the field Volume of the sample, whichever is smaller.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTemperature

    The temperature of the device that is used to mix/incubate the sample. If mixing via homogenization, the pulse duty cycle of the sonication horn is automatically adjusted if the measured temperature of the sample exceeds this set temperature.
    Default Value: Automatic
    Default Calculation: Automatically set to 40 Celsius if AnnealingTime is specified, if not, set to Ambient.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTemperatureProfile

    The temperature of the device, over the course of time, that is used to mix/incubate the sample.
    Default Value: Automatic
    Pattern Description: List of one or more {Time, Temperature} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Minute, $MaxExperimentTime], RangeP[$MinTemperatureProfileTemperature, $MaxTemperatureProfileTemperature]}..}) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixMaxTemperature

    The maximum temperature that the sample is allowed to reach during mixing via homogenization or sonication. If the measured temperature is above this MaxTemperature, the homogenizer/sonicator turns off until the measured temperature is 2C below the MaxTemperature, then it automatically resumes.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees Celsius and less than or equal to 100 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[0*Celsius, 100*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixOscillationAngle

    The angle of oscillation of the mixing motion when a wrist action shaker is used.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 15 degrees or Null.
    Programmatic Pattern: ((Null | RangeP[0*AngularDegree, 15*AngularDegree]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixAmplitude

    The amplitude of the sonication horn when mixing via homogenization. When using a microtip horn (ex. for 2mL and 15mL tubes), the maximum amplitude is 70 Percent, as specified by the manufacturer, in order not to damage the instrument.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 10 percent and less than or equal to 100 percent or Null.
    Programmatic Pattern: ((Null | RangeP[10*Percent, 100*Percent]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixFlowRate

    The speed at which liquid is aspirated and dispensed in a liquid before it is aspirated. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 100 Microliter/Second when mixing by pipetting if Preparation->Robotic.
    Pattern Description: MixFlowRate speed at which liquid should be drawn up into the pipette tip or Null.
    Programmatic Pattern: ((Null | RangeP[0.4*(Microliter/Second), 500*(Microliter/Second)]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixPosition

    The location from which liquid should be mixed by pipetting. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to LiquidLevel if MixType->Pipette and Preparation->Robotic.
    Pattern Description: MixPosition location from which liquid should be aspirated or Null.
    Programmatic Pattern: ((Null | PipettingPositionP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixPositionOffset

    The distance from the center of the well that liquid will aspirated/dispensed while mixing the sample. When specifying a Z Offset, the Z Offset is calculated either as the height below the top of the well, the height above the bottom of the well, or the height below the detected liquid level, depending on value of the AspirationPosition option (Top|Bottom|LiquidLevel). When an AspirationAngle is specified, the AspirationPositionOffset is measured in the frame of reference of the tilted labware (so that wells that are further away from the pivot point of the tilt are in the same frame of reference as wells that are close to the pivot point of the tilt). This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to 2 Millimeter if MixType->Pipette and Preparation->Robotic.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixCorrectionCurve

    The relationship between a target volume and the corrected volume that needs to be aspirated or dispensed to reach the target volume. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to correction curve set in the PipettingMethod for the Object[Sample] if MixType->Pipette and Preparation->Robotic.
    Pattern Description: List of one or more {Target Volume, Actual Volume} entries or Null.
    Programmatic Pattern: ((Null | {{RangeP[0*Microliter, 1000*Microliter], RangeP[0*Microliter, 1250*Microliter]}..}) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTips

    The pipette tips used to aspirate and dispense the requested volume..
    Default Value: Automatic
    Default Calculation: If MixType->Pipette, automatically set to the preferred tips determined by TransferDevices with given MixVolume, as well as TipType and TipMaterial if specified, and it is also checked that the tips can reach the bottom of the container.
    Pattern Description: An object of type or subtype Model[Item, Tips] or Object[Item, Tips] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Tips], Object[Item, Tips]}]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTipType

    The tip type to use to mix liquid in the manipulation.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field TipType of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the transfer.
    Default Value: Automatic
    Default Calculation: If Tips is specified, automatically set to the field Material of the Model[Item, Tips] or the Model of Object[Item,Tips]. Otherwise, set to Null.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixMultichannelMix

    Indicates if multiple device channels should be used when performing pipette mixing.
    Default Value: Automatic
    Default Calculation: Automatically set to True if there are multiple samples that are mixed via pipette, if MixType->Pipette.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixDeviceChannel

    The channel of the work cell that should be used to perform the pipetting mixing. This option can only be set if Preparation->Robotic and MixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to SingleProbe1 if MultichannelMix->False. Otherwise, set to the appropriate channel to perform the transfer, if Preparation->Robotic and MixType->Pipette.
    Pattern Description: MultiProbeHead, SingleProbe1, SingleProbe2, SingleProbe3, SingleProbe4, SingleProbe5, SingleProbe6, SingleProbe7, or SingleProbe8 or Null.
    Programmatic Pattern: ((Null | DeviceChannelP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixResidualIncubation

    Indicates if the incubation and/or mixing should continue after Time/MaxTime has finished while waiting to progress to the next step in the protocol.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Temperature is non-Ambient and the samples being incubated have non-ambient TransportTemperature.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixResidualTemperature

    The temperature at which the sample(s) should remain incubating after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to Temperature if ResidualIncubation is True and Preparation->Robotic.
    Pattern Description: Ambient or Null or greater than or equal to 0 degrees Celsius and less than or equal to 105 degrees Celsius.
    Programmatic Pattern: ((RangeP[0*Celsius, 105*Celsius] | (Ambient | Null)) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixResidualMix

    Indicates that the sample(s) should remain shaking at the ResidualMixRate after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixResidualMixRate

    When mixing by shaking, this is the rate at which the sample(s) remains shaking after Time has elapsed. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to MixRate if ResidualMix is True and Preparation->Robotic.
    Pattern Description: Greater than or equal to 30 revolutions per minute and less than or equal to 2500 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[30*RPM, 2500*RPM]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixPreheat

    Indicates if the incubation position is brought to Temperature before exposing the Sample to it. This option can only be set if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Preparation->Robotic.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Transfer Technique

    SterileTechnique

    Indicates if 70% ethanol will be sprayed on all surfaces/containers used during the transfer. This also indicates that sterile instruments and a sterile transfer environments must be used for the transfer. Please consult the ExperimentTransfer documentation for a full diagram of SterileTechnique that is employed by operators.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the samples being transferred contain tissue culture or microbial components.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

Cover

    Sample

    The sample who should be covered.
    Pattern: ListableP[NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}]

    General

    Sample

    The samples or containers that should be covered.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ObjectP[{Object[Sample], Object[Container]}] | _String
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the samples that are being covered, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the containers of the samples that are being covered, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    CoverType

    The type of cover (Crimp, Seal, Screw, Snap, AluminumFoil, Pry, or Place) that should be used to cover the container.
    Default Value: Automatic
    Default Calculation: Automatically set to the first cover type in the field CoverTypes from the Model of the Object[Container] that is to be covered.
    Pattern Description: Crimp, Seal, Screw, Snap, Place, Pry, or AluminumFoil or Null.
    Programmatic Pattern: ((Null | CoverTypeP) | Automatic) | Null
    Index Matches to: Sample

    UsePreviousCover

    Indicates if the previous cover should be used to re-cover this container. Note that the previous cover cannot be used if it is discarded or if CoverType->Crimp|Seal.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the PreviousCover field is set in Object[Container] and the PreviousCover is not Discarded or on another container.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    Opaque

    Indicates if an opaque cover is used to cover the container.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Cover that is specified by the user. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Cover

    The cap, lid, or plate seal that should be secured to the top of the given container.
    Default Value: Automatic
    Default Calculation: Automatically set to a cover that matches the calculated CoverType, Opaque, and UsePreviousCover options. This cover must also be compatible with the given container's CoverFootprints.
    Pattern Description: An object of type or subtype Model[Item, Lid], Object[Item, Lid], Model[Item, Cap], Object[Item, Cap], Model[Item, PlateSeal], or Object[Item, PlateSeal] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Item, Lid], Object[Item, Lid], Model[Item, Cap], Object[Item, Cap], Model[Item, PlateSeal], Object[Item, PlateSeal]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    CoverLabel

    A user defined word or phrase used to identify the covers that are being used in the experiment, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    Septum

    The septum that is used in conjunction with the cover to secure the top of the given container.
    Default Value: Automatic
    Default Calculation: Automatically set a Model[Item, Septum] with the same CoverFootprint as the calculated Cover, if CoverType->Crimp and the calculated Cover requires a septum (SeptumRequired->True in Model[Item, Cap]).
    Pattern Description: An object of type or subtype Model[Item, Septum] or Object[Item, Septum] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Septum], Object[Item, Septum]}]) | Automatic) | Null
    Index Matches to: Sample

    Stopper

    The stopper that is used in conjunction with the crimped cover and septum to secure the top of the given container.
    Default Value: Null
    Pattern Description: An object of type or subtype Model[Item, Stopper] or Object[Item, Stopper] or Null.
    Programmatic Pattern: (Null | ObjectP[{Model[Item, Stopper], Object[Item, Stopper]}]) | Null
    Index Matches to: Sample

    Instrument

    The device used to help secure the cover to the top of the container.
    Figure 3.19: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to a Model[Instrument, Crimper] that has the same CoverFootprint as the calculated Cover if CoverType->Crimp or set to a Model[Instrument, PlateSealer] that has the same CoverFootprint as the calculated Cover if CoverType->Seal. Otherwise, is set to Null.
    Pattern Description: An object of type or subtype Model[Instrument, Crimper], Object[Instrument, Crimper], Model[Instrument, PlateSealer], or Object[Instrument, PlateSealer] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Crimper], Object[Instrument, Crimper], Model[Instrument, PlateSealer], Object[Instrument, PlateSealer]}]) | Automatic) | Null
    Index Matches to: Sample

    CrimpingHead

    The part that is attached to the Object[Instrument, Crimper] that transfers the pneumatic pressure from the crimping instrument to secure the crimped cap to the top of the container.
    Default Value: Automatic
    Default Calculation: Automatically set to a Model[Part, CrimpingHead] that has the same with the CoverFootprint and CrimpType as the Cover, if the Cover is a Model[Item,Cap] with CoverType->Crimp.
    Pattern Description: An object of type or subtype Model[Part, CrimpingHead] or Object[Part, CrimpingHead] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, CrimpingHead], Object[Part, CrimpingHead]}]) | Automatic) | Null
    Index Matches to: Sample

    DecrimpingHead

    Used in conjunction with the CrimpingHead to remove the crimped cap from the covered container, if the crimp was not successful. Successful crimps are (1) level with the bottom of the container and (2) not over or under tightened to the top of the container. If the previous crimping attempt is not successful, the operator will decrimp and recrimp until they have a successful crimp.
    Default Value: Automatic
    Default Calculation: Automatically set to a Model[Part, DecrimpingHead] that has the same with the CoverFootprint as the Cover, if the Cover is a Model[Item,Cap] with CoverType->Crimp.
    Pattern Description: An object of type or subtype Model[Part, DecrimpingHead] or Object[Part, DecrimpingHead] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, DecrimpingHead], Object[Part, DecrimpingHead]}]) | Automatic) | Null
    Index Matches to: Sample

    CrimpingPressure

    The pressure of the gas that is connected to the pneumatic Model[Instrument, Crimper] that determines the strength used to crimp or decrimp the crimped cap.
    Default Value: Automatic
    Default Calculation: Automatically set to the CrimpingPressure field in the Model[Item, Cap] if CoverType->Crimp. If this field is empty, set to 20 PSI. Otherwise, if CoverType is not Crimp, set to Null.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 90 pounds‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 90*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Temperature

    The temperature that will be used to heat the seal for sealing a plate when using heat-activated adhesive seal.
    Default Value: Automatic
    Default Calculation: Automatically set to 180 Celsius or 185 Celsius if the Cover option resolves to a heat-activated adhesive plate seal .
    Pattern Description: Greater than or equal to 100 degrees Celsius and less than or equal to 190 degrees Celsius or Null.
    Programmatic Pattern: ((Null | RangeP[100*Celsius, 190*Celsius]) | Automatic) | Null
    Index Matches to: Sample

    Time

    The duration of time used for applying Temperature to seal the plate when using heat-activated adhesive seal.
    Default Value: Automatic
    Default Calculation: Automatically set to 3.5 Second if the Cover option resolves to a heat-activated adhesive plate seal for SBS plates.
    Pattern Description: Greater than or equal to 0.5 seconds and less than or equal to 10 seconds or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Second, 10*Second]) | Automatic) | Null
    Index Matches to: Sample

    Parafilm

    Indicates if Parafilm should be used to secure the cover after it is attached to the container.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Parafilm field in Model[Container], Object[Container], Model[Sample] or Object[Sample]. If the field in these objects is not set, parafilm is not used unless explicitly specified by the user.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    KeckClamp

    The Keck Clamp that is used to secure a tapered stopper Cover to the tapered ground glass joint opening of the Container.
    Default Value: Automatic
    Default Calculation: If the Cover has a specified TaperGroundJointSize, automatically set to a Model[Item, Clamp] that has the same TaperGroundJointSize.
    Pattern Description: An object of type or subtype Model[Item, Clamp] or Object[Item, Clamp] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, Clamp], Object[Item, Clamp]}]) | Automatic) | Null
    Index Matches to: Sample

    PlateSealAdapter

    Used to raise the SBS-format microplate inside of a PlateSealer instrument so that the plate is able to reach the height requirement of PlateSealer.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container, Rack] or Object[Container, Rack] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Rack], Object[Container, Rack]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PlateSealPaddle

    Used to apply adhesive PlateSeal to the top of the plate firmly.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Item, PlateSealRoller] or Object[Item, PlateSealRoller] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Item, PlateSealRoller], Object[Item, PlateSealRoller]}]) | Automatic) | Null
    Index Matches to: Sample

    AluminumFoil

    Indicates if Aluminum Foil should be wrapped around the entire container after the cover is attached in order to protect the container's contents from light. Note that this option is NOT to use an aluminum foil cover; in order to specify that, CoverType must be set to AluminumFoil.
    Default Value: Automatic
    Default Calculation: Automatically set based on the AluminumFoil field in Model[Container], Object[Container], Model[Sample] or Object[Sample]. If the field in these objects is not set, aluminum foil is not used unless explicitly specified by the user.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    KeepCovered

    Indicates if the cover on this container should be "peaked" off when transferred into/out of instead of taken off completely when performing Manual Transfers. When performing robotic manipulations, this indicates that the container should be re-covered after any manipulation that uncovers it is completed.
    Default Value: Automatic
    Default Calculation: Automatically set based on the KeepCovered field in Object[Container]. If the field in this object is not set, the option will be set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    Environment

    The environment in which the covering should be performed (Biosafety Cabinet, Fume Hood, Glove Box, or Bench). This option will be set to Null when Preparation->Robotic (the covering will be performed inside of the Liquid Handler enclosure).
    Default Value: Automatic
    Default Calculation: Automatically set to the specific BSC, fume hood, glove box, or bench that the sample is currently on (if applicable). Otherwise, if SterileTechnique->True, this option will be set to a BSC. Otherwise, defaults to a fume hood.
    Pattern Description: An object of type or subtype Model[Instrument], Model[Container, Bench], Model[Container, OperatorCart], Object[Instrument], Object[Container, Bench], or Object[Container, OperatorCart] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument], Model[Container, Bench], Model[Container, OperatorCart], Object[Instrument], Object[Container, Bench], Object[Container, OperatorCart]}]) | Automatic) | Null
    Index Matches to: Sample

    SterileTechnique

    Indicates if 70% ethanol will be sprayed on all surfaces/containers used during the transfer. This also indicates that sterile instruments and a sterile transfer environments must be used for the transfer. Please consult the ExperimentTransfer documentation for a full diagram of SterileTechnique that is employed by operators.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the samples being transferred contain tissue culture or microbial components.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Uncover

    Sample

    The sample who should be uncovered.
    Pattern: ListableP[NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}]

    General

    Sample

    The samples or containers that should be uncovered.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ObjectP[{Object[Sample], Object[Container]}] | _String
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the sample which is uncovered, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the container of the sample which is uncovered, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    DiscardCover

    Indicates if the cover is discarded after it's taken off of the container.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Reusable field of the cover and the UncappedTime. All caps that are not Reusable will always be discarded. Reusable caps will be discarded if the UncappedTime is greater than 15 minutes so that fresh caps can be applied after processing.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    Instrument

    The device used to remove the cover from the top of the container.
    Figure 3.20: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically set to a Model[Instrument, Crimper] that has the same CoverFootprint as Cover, if using a crimped cover. Automatically set to Model[Part, CapPrier] if CoverType->Pry. Otherwise, set to Null.
    Pattern Description: An object of type or subtype Model[Instrument, Crimper], Object[Instrument, Crimper], Model[Part, CapPrier], Object[Part, CapPrier], Model[Part, Decrimper], or Object[Part, Decrimper] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Crimper], Object[Instrument, Crimper], Model[Part, CapPrier], Object[Part, CapPrier], Model[Part, Decrimper], Object[Part, Decrimper]}]) | Automatic) | Null
    Index Matches to: Sample

    DecrimpingHead

    Used in conjunction with a Model[Instrument, Crimper] to remove the crimped cap from the covered container. Note that this is not used if using a handheld Model[Part, Decrimper].
    Default Value: Automatic
    Default Calculation: Automatically set to a Model[Part, DecrimpingHead] that has the same CoverFootprint as the Cover, if the Cover is a Model[Item, Cap] with CoverType->Crimp, and if not using a manual decrimper.
    Pattern Description: An object of type or subtype Model[Part, DecrimpingHead] or Object[Part, DecrimpingHead] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Part, DecrimpingHead], Object[Part, DecrimpingHead]}]) | Automatic) | Null
    Index Matches to: Sample

    CrimpingPressure

    The pressure of the gas that is connected to the pneumatic Model[Instrument, Crimper] that determines the strength used to crimp or decrimp the crimped cap. Note that this is not used if using a handheld Model[Part, Decrimper].
    Default Value: Automatic
    Default Calculation: Automatically set to the CrimpingPressure field in the Model[Item, Cap] if CoverType->Crimp. If this field is empty, set to 35 PSI. Otherwise, if CoverType is not Crimp, set to Null.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 90 pounds‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, 90*PSI]) | Automatic) | Null
    Index Matches to: Sample

    Environment

    The environment in which the covering should be performed (Biosafety Cabinet, Fume Hood, Glove Box, or Bench). This option will be set to Null when Preparation->Robotic (the covering will be performed inside of the Liquid Handler enclosure).
    Default Value: Automatic
    Default Calculation: Automatically set to the specific BSC, fume hood, glove box, or bench that the sample is currently on (if applicable). Otherwise, if SterileTechnique->True, this option will be set to a BSC. Otherwise, defaults to a fume hood.
    Pattern Description: An object of type or subtype Model[Instrument], Model[Container, Bench], Model[Container, OperatorCart], Object[Instrument], Object[Container, Bench], or Object[Container, OperatorCart] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument], Model[Container, Bench], Model[Container, OperatorCart], Object[Instrument], Object[Container, Bench], Object[Container, OperatorCart]}]) | Automatic) | Null
    Index Matches to: Sample

    SterileTechnique

    Indicates if 70% ethanol will be sprayed on all surfaces/containers used during the transfer. This also indicates that sterile instruments and a sterile transfer environments must be used for the transfer. Please consult the ExperimentTransfer documentation for a full diagram of SterileTechnique that is employed by operators.
    Default Value: Automatic
    Default Calculation: Automatically set to True if the samples being transferred contain tissue culture or microbial components.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

SerialDilute

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Source

    The samples that should be serially diluted.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: (ObjectP[{Object[Sample], Object[Container]}] | _String) | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]] | LocationPositionP, ObjectP[{Object[Container]}] | _String}
    Index Matches to: Source

    SourceLabel

    The label of the source sample that is used in the sequential dilution.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    SourceContainerLabel

    The label of the source sample's container that is used for the transfer.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Source

    SerialDilutionFactors

    The factors by which you wish to reduce the concentrations starting with SamplesIn, followed by each previous dilution in the series of dilutions. This or TargetConcentrations must be provided, or an error is thrown.
    Default Value: Automatic
    Default Calculation: Automatically set to 10. This option can also be calculated from equations in Process Diagrams.
    Pattern Description: List of one or more greater than or equal to 1 entries or Null.
    Programmatic Pattern: ((Null | {GreaterEqualP[1]..}) | Automatic) | Null
    Index Matches to: Source

    NumberOfSerialDilutions

    The number of times the sample is repeatedly diluted, starting with SamplesIn, followed by each previous dilution.
    Default Value: Automatic
    Default Calculation: Automatically set to the number of TargetConcentrations or SerialDilutionFactors if provided, otherwise set to 1.
    Pattern Description: Greater than or equal to 1 in increments of 1.
    Programmatic Pattern: GreaterEqualP[1, 1] | Automatic
    Index Matches to: Source

    TargetConcentrations

    Desired concentrations of Analyte in the final diluted samples after serial dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set to 2Molar. In either Direct or FromConcentrate, this can be calculated from the equations in Process Diagrams.
    Pattern Description: List of one or more greater than 0 molar or greater than 0 grams per liter entries or Null.
    Programmatic Pattern: ((Null | {(GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])..}) | Automatic) | Null
    Index Matches to: Source

    Analyte

    The components in SamplesIn's Composition whose final concentrations (TargetConcentrations) are attained through a series of repeated dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of SamplesIn, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Source

    FinalVolume

    The volume into which the sample is diluted for each serial dilution.
    Default Value: Automatic
    Default Calculation: Automatically set to 100Microliter. In either Direct or FromConcentrate, can be calculated from the equations in Process Diagrams.
    Pattern Description: List of one or more greater than or equal to 0 microliters and less than or equal to 20 liters entries.
    Programmatic Pattern: {RangeP[0*Microliter, $MaxTransferVolume]..} | Automatic
    Index Matches to: Source

    BufferDilutionStrategy

    BufferDilutionStrategy describes the manner in which to generate to buffer samples for each serial dilution. FromConcentrate provides ConcentratedBuffer to each well, which is then diluted with BufferDiluent to reach a final buffer concentraion of 1X, whereas Direct uses pre-diluted buffer which is already at 1X to perform the subsequent dilutions (see Equations chart in Process Diagrams).
    Default Value: Direct
    Pattern Description: Direct or FromConcentrate.
    Programmatic Pattern: Direct | FromConcentrate
    Index Matches to: Source

    TransferAmounts

    The series of volume transferred starting from SamplesIn, and going into each subsequent dilution.
    Default Value: Automatic
    Default Calculation: Automatically set to 10Microliter. If SerialDilutionFactors or TargetConcentrations, and FinalVolume are provided, this option can be calculated from the equations in Process Diagrams.
    Pattern Description: List of one or more greater than or equal to 0 microliters and less than or equal to 20 liters entries.
    Programmatic Pattern: {RangeP[0*Microliter, $MaxTransferVolume]..} | Automatic
    Index Matches to: Source

    Diluent

    The solution used to reduce the concentration of the SamplesIn and each subsequent dilution.
    Default Value: Automatic
    Default Calculation: Automatically set to the Solvent of SamplesIn, or Model[Sample,"milli-Q Water"] if Solvent is not defined.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Source

    DiluentLabel

    The label of the diluent added to the diluted sample.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    DiluentAmount

    The amount of solution used to reduce the concentration of the SamplesIn and each subsequent dilution.
    Default Value: Automatic
    Default Calculation: Automatically set to 90Microliter. If SerialDilutionFactors or TargetConcentrations, and FinalVolume are provided, this option can be calculated from the equations in Process Diagrams.
    Pattern Description: List of one or more greater than or equal to 0 microliters and less than or equal to 20 liters entries.
    Programmatic Pattern: {RangeP[0*Microliter, $MaxTransferVolume]..} | Automatic
    Index Matches to: Source

    BufferDiluent

    The solution used to reduce the concentration of the ConcentratedBuffer in the first and each subsequent dilution.
    Default Value: Null
    Default Calculation: If the BufferDilutionStrategy is Direct, it will be set to Null. If not, it will automatically be set to the Solvent of ConcentratedBuffer, or Model["milli-Q Water"] if Solvent is not defined.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Source

    BufferDiluentLabel

    The label of the buffer used to dilute the sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    BufferDilutionFactor

    The factor by which to reduce the concentration of the ConcentratedBufferDilutionFactor (but not the input sample) in all of the dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set to the ConcentratedBufferDilutionFactor of ConcentratedBuffer. If ConcentratedBufferDilutionFactor is not defined, automatically set to 10 and throws a warning.
    Pattern Description: Greater than or equal to 1.
    Programmatic Pattern: GreaterEqualP[1] | Automatic
    Index Matches to: Source

    BufferDiluentAmount

    The amount of buffer diluent to be added to reduce the concentration of ConcentratedBuffer in each dilution.
    Default Value: Automatic
    Default Calculation: Can be calculated from the equations in Process Diagrams.
    Pattern Description: List of one or more greater than or equal to 0 microliters and less than or equal to 20 liters entries or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Microliter, $MaxTransferVolume]..}) | Automatic) | Null
    Index Matches to: Source

    ConcentratedBuffer

    The buffer used in the first dilution from SamplesIn. If BufferDiluentStrategy is FromConcentrate, then it will also be used in subsequent dilutions.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Source

    ConcentratedBufferLabel

    The label of the concentrated buffer that is diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent).
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Source

    ConcentratedBufferAmount

    The amount of ConcentratedBuffer used in the first dilution from SamplesIn and each subsequent dilution.
    Default Value: Automatic
    Default Calculation: Can be calculated from the equations in Additional Information.
    Pattern Description: List of one or more greater than or equal to 0 microliters and less than or equal to 20 liters entries or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Microliter, 20*Liter]..}) | Automatic) | Null
    Index Matches to: Source

    DiscardFinalTransfer

    Indicates if the final wells contain the same volume as the previously diluted wells by removing TransferAmount from the final dilution.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Source

    DestinationWells

    The wells in which the dilutions will occur in ContainerOut if the dilutions occur in well plates.
    Default Value: Automatic
    Default Calculation: Automatically determined based on available wells and volume of liquid.
    Pattern Description: List of one or more any well from A1 to P24 entries or Null.
    Programmatic Pattern: ((Null | {WellPositionP..}) | Automatic) | Null
    Index Matches to: Source

    ContainerOut

    The desired type of container that should be used to prepare and house the diluted samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before diluting into the next.
    Pattern Description: List of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample entries or list of one or more {Index, Container} entries.
    Programmatic Pattern: ({(ObjectP[{Model[Container], Object[Container]}] | _String)..} | {{GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}..}) | Automatic
    Index Matches to: Source

    SampleOutLabel

    The label of the sample(s) that become the SamplesOut.
    Default Value: Automatic
    Pattern Description: List of one or more a string entries.
    Programmatic Pattern: {_String..} | Automatic
    Index Matches to: Source

    ContainerOutLabel

    The label of the container that holds the sample that becomes the SamplesOut.
    Default Value: Automatic
    Pattern Description: List of one or more a string entries.
    Programmatic Pattern: {_String..} | Automatic
    Index Matches to: Source

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Mixing

    TransferMix

    Indicates if mixing of the samples is needed after each dilution in the series.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Source

    TransferMixType

    Determines which type of mixing should be performed on the diluted samples after each dispense.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Volume option and size of the container in which the sample is prepared.
    Pattern Description: Pipette or Swirl or Null.
    Programmatic Pattern: ((Null | (Pipette | Swirl)) | Automatic) | Null
    Index Matches to: Source

    TransferNumberOfMixes

    Determines the number of times the sample is mixed for discrete mixing processes such as Pipette or Swirl.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[0, 1]) | Automatic) | Null
    Index Matches to: Source

    Incubate

    Determines if incubation of the samples should occur after the dilutions.
    Default Value: True
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null
    Index Matches to: Source

    Incubation and Mixing

    IncubationTime

    Determines how long the incubation should occur for the sample after the dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 minutes unless MixType is set to Pipette, Swirl or Invert, in which case it is set to Null.
    Pattern Description: Greater than or equal to 0 hours and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Hour, 72*Hour]) | Automatic) | Null
    Index Matches to: Source

    MaxIncubationTime

    The maximum time to let the samples incubate after the dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set based on the MixType if MixUntilDissolved is set to True. If MixUntilDissolved is False, resolves to Null.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Second, 72*Hour]) | Automatic) | Null
    Index Matches to: Source

    IncubationTemperature

    The temperature at which the diluted samples will incubate after the dilutions.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient, or Null if Mix is set to False or MixType is set to Pipette, Swirl or Invert.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Source

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Source

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Source

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Resuspend

    resuspendRules

    The list of key/value pairs specifying the samples and volume of solvent to transfer into the sample.
    Pattern: {Source -> NonSelfContainedSampleP | NonSelfContainedSampleModelP | ObjectP[{Model[Container, Vessel], Model[Container, ReactionVessel], Model[Container, Cuvette], Object[Container, Vessel], Object[Container, ReactionVessel], Object[Container, Cuvette]}] | {ObjectP[{Model[Container, Plate], Object[Container, Plate]}], WellP}, Volume -> GreaterEqualP[0*Milliliter]}

    General

    Sample

    The samples that should be resuspended.
    Default Value: Null
    Pattern Description: Sample or Container or Null.
    Programmatic Pattern: Alternatives[ObjectP[{Object[Sample], Object[Container]}] | _String]
    Index Matches to: Sample

    Amount

    The amount of a sample that should be resuspended in a diluent.
    Default Value: Automatic
    Default Calculation: Automatically set to the current mass of the sample, or the value necessary to reach the specified TargetConcentration.
    Pattern Description: All or Count or Count or Mass or Null.
    Programmatic Pattern: ((Null | (RangeP[0*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte after resuspension of the input samples with the Diluent.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on Amount and Volume options.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    Volume

    The desired total volume of the resuspended sample plus diluent.
    Default Value: Automatic
    Default Calculation: Automatically determined based on the TargetConcentration option values. If not specified and TargetConcentration is also not specified, an error is thrown.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ContainerOut

    The desired type of container that should be used to prepare and house the resuspended samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set to the current container of the input sample.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container}
    Programmatic Pattern: ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding ContainerOut in which the resuspended samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to the current position of the input sample if ContainerOut is the current container of the input sample. Otherwise set to A1 in vessels, or an empty well of the specified plate.
    Pattern Description: Any well from A1 to H12.
    Programmatic Pattern: WellPositionP | Automatic
    Index Matches to: Sample

    Diluent

    The sample that should be added to the input sample, where the volume of this sample added is the Volume option.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise set to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total Volume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total Volume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the total Volume.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    SampleLabel

    The label of the sample that is used for the dilution.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    The label of the ssample's container that is used for the dilution.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleOutLabel

    The label of the sample(s) that become the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    ContainerOutLabel

    The label of the container that holds the sample that becomes the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    DiluentLabel

    The label of the diluent added to the sample.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBufferLabel

    The label of the concentrated buffer that is diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent).
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluentLabel

    The label of the buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Incubation and Mixing

    Mix

    Indicates if mixing of the resuspended samples will occur following the addition of liquid.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample

    MixType

    The style of motion used to mix the resuspended samples solution following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Volume option and size of the container in which the sample is prepared.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    NumberOfMixes

    Determines the number of times the sample is mixed for discrete mixing processes such as Pipette or Invert.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[0, 1]) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the sample should be mixed in an attempt to completely dissolve any solid components following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxMixTime is specified, or False otherwise.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    The duration for which the sample should be mixed/incubated following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 minutes unless MixType is set to Pipette, Swirl or Invert, in which case it is set to Null.
    Pattern Description: Greater than or equal to 0 hours and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Hour, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    The maximum duration for which the samples should be mixed/incubated in an attempt to dissolve any solid components following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set based on the MixType if MixUntilDissolved is set to True. If MixUntilDissolved is False, resolves to Null.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Second, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument that should be used to mix/incubate the sample following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to an appropriate instrument based on container model and MixType, or Null if Mix is set to False.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the sample should be mixed/incubated for the duration of the IncubationTime following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient, or Null if Mix is set to False or MixType is set to Pipette, Swirl or Invert.
    Pattern Description: Ambient or greater than or equal to 22 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[22*Celsius, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the sample should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed if mixing while incubating.
    Default Value: Automatic
    Default Calculation: Automatically resolves to 0 Minute, or Null if Mix is set to False.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    MixOrder

    Indicates if mixing/incubating are done for one sample after addition of liquid before advancing to the next (Serial) or all at once after liquid is added to all samples (Parallel).
    Default Value: Parallel
    Pattern Description: Serial or Parallel.
    Programmatic Pattern: ReadOrderP

    Post Experiment

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Dilute

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that should be diluted.
    Default Value: Null
    Pattern Description: Sample or Container or Null.
    Programmatic Pattern: Alternatives[ObjectP[{Object[Sample], Object[Container]}] | _String]
    Index Matches to: Sample

    Amount

    The amount of a sample that should be diluted with a diluent.
    Default Value: Automatic
    Default Calculation: Automatically set to the current volume of the sample, or the value necessary to reach the specified TargetConcentration.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[0*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte after dilution of the input samples with the Diluent.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on Amount and Volume options.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    TotalVolume

    The desired total volume of the sample plus diluent.
    Default Value: Automatic
    Default Calculation: Automatically determined based on the TargetConcentration option values. If not specified and TargetConcentration is also not specified, an error is thrown.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample

    ContainerOut

    The desired type of container that should be used to prepare and house the diluted samples, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set to the current container of the input sample.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container}
    Programmatic Pattern: ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic
    Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding ContainerOut in which the resuspended samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically set to the current position of the input sample if ContainerOut is the current container of the input sample. Otherwise set to A1 in vessels, or an empty well of the specified plate.
    Pattern Description: Any well from A1 to H12.
    Programmatic Pattern: WellPositionP | Automatic
    Index Matches to: Sample

    Diluent

    The sample that should be added to the input sample, where the volume of this sample added is the TotalVolume option - the current sample volume.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise set to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the TotalVolume.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: (Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Null
    Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the TotalVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1]) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the Amount and the TotalVolume.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    SampleLabel

    The label of the sample that is used for the dilution.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    The label of the ssample's container that is used for the dilution.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleOutLabel

    The label of the sample(s) that become the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    ContainerOutLabel

    The label of the container that holds the sample that becomes the SamplesOut.
    Default Value: Automatic
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    DiluentLabel

    The label of the diluent added to the sample.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    ConcentratedBufferLabel

    The label of the concentrated buffer that is diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent).
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    BufferDiluentLabel

    The label of the buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Incubation and Mixing

    Mix

    Indicates if mixing of the diluted samples will occur.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample

    MixType

    The style of motion used to mix the diluted samples solution following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set based on the Volume option and size of the container in which the sample is prepared.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample

    NumberOfMixes

    Determines the number of times the sample is mixed for discrete mixing processes such as Pipette or Invert.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[0, 1]) | Automatic) | Null
    Index Matches to: Sample

    MixUntilDissolved

    Indicates if the sample should be mixed in an attempt to thoroughly mix the sample with the diluent.
    Default Value: Automatic
    Default Calculation: Automatically set to True if MaxIncubationTime is specified, or False otherwise.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample

    IncubationTime

    The duration for which the sample should be mixed/incubated following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 minutes unless MixType is set to Pipette, Swirl or Invert, in which case it is set to Null.
    Pattern Description: Greater than or equal to 0 hours and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Hour, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    MaxIncubationTime

    The maximum duration for which the samples should be mixed/incubated in an attempt to dissolve any solid components following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set based on the MixType if MixUntilDissolved is set to True. If MixUntilDissolved is False, resolves to Null.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Second, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    IncubationInstrument

    The instrument that should be used to incubate the sample following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to an appropriate instrument based on container model and MixType, or Null if Mix is set to False.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample

    IncubationTemperature

    Temperature at which the sample should be mixed/incubated for the duration of the IncubationTime following addition of liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient, or Null if Mix is set to False or MixType is set to Pipette, Swirl or Invert.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the sample should remain in the incubator allowing the system to settle to room temperature after the MixTime has passed if mixing while incubating.
    Default Value: Automatic
    Default Calculation: Automatically resolves to 0 Minute, or Null if Mix is set to False.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, 72*Hour]) | Automatic) | Null
    Index Matches to: Sample

    Post Experiment

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    Mixing

    MixOrder

    Indicates if mixing/incubating are done for one sample after addition of liquid before advancing to the next (Serial) or all at once after liquid is added to all samples (Parallel).
    Default Value: Parallel
    Pattern Description: Serial or Parallel.
    Programmatic Pattern: ReadOrderP

MagneticBeadSeparation

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    Input samples which will be isolated via magnetic bead separation.
    Default Value: Null
    Pattern Description: Container with Well Position or Sample or Container or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Object[Container]}] | _String) | {WellPositionP, ObjectP[{Object[Container]}] | _String}) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Volume

    The amount of sample that is added to the magnetic beads in order to allow binding of target analyte or contaminant to the magnetic beads after the magnetic beads are optionally prewashed and equilibrated.
    Default Value: Automatic
    Default Calculation: Automatically set to the sample volume (aliquoted if specified NumberOfReplicates) if it is below 25 mL for manual preparation or below 1 mL for robotic preparation. Otherwise, set to 25 mL for manual preparation or to 1 mL for robotic preparation.
    Pattern Description: All or Volume.
    Programmatic Pattern: (RangeP[1*Microliter, 50*Milliliter] | All) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Target

    The molecule or analyte that we aim to purify from the SamplesIn.
    Default Value: Automatic
    Default Calculation: Automatically set to the first item in Analytes of the sample if analyteAffinityLabel is not specified. Otherwise, Target is set to analyteAffinityLabel if SelectionStrategy->Positive, and is set to the first non-analyteAffinityLabel item in Analytes of the sample if SelectionStrategy->Negative.
    Pattern Description: An object of type or subtype Model[Molecule] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Molecule]]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AnalyteAffinityLabel

    The target molecule in the sample that binds the immobilized ligand on the magnetic beads for affinity separation, applicable if SeparationMode is set to Affinity. AnalyteAffinityLabel is used to help set automatic options such as MagneticBeadAffinityLabel.
    Default Value: Automatic
    Default Calculation: Automatically set to match the Target if SelectionStrategy is set to Positive, and is set to the first non-Target item in Analytes of the sample if SelectionStrategy is set to Negative.
    Pattern Description: An object of type or subtype Model[Molecule] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Molecule]]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MagneticBeadAffinityLabel

    The molecule immobilized on the magnetic beads that specifically binds the target analyte for affinity separation, applicable if SeparationMode is set to Affinity. MagneticBeadAffinityLabel is used to help set automatic options such as MagneticBeads.
    Default Value: Automatic
    Default Calculation: Automatically set to the first item in the Targets field of the AnalyteAffinityLabel if SeparationMode is set to Affinity. For example, if the AnalyteAffinityLabel is a biotinylated molecule, that molecule's Targets field is set to streptavidin.
    Pattern Description: An object of type or subtype Model[Molecule] or Null.
    Programmatic Pattern: ((Null | ObjectP[Model[Molecule]]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MagneticBeads

    The superparamagnetic particles with surface coatings to bind target analyte or contaminants. They exhibit magnetic behavior only in the presence of an external magnetic field. The magnetic beads are pulled to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the supernatant.
    Default Value: Automatic
    Default Calculation: If SeparationMode is Affinity, MagneticBeads is automatically set to the first found magnetic beads model with the affinity label of MagneticBeadAffinityLabel. Otherwise, MagneticBeads is automatically set according to Target and Separation Mode. If Target is a nucleic acid (DNA, RNA, cDNA, plasmid, etc.), MagneticBeads is automatically set to Model[Sample, "Dynabeads MyOne SILANE Sample"]. If Target is a protein or peptide, MagneticBeads is automatically set to Model[Sample,"MagSi-proteomics C4"] when SeparationMode is ReversePhase, and set to Model[Sample,"MagSi-WCX"] when SeparationMode is IonExchange. Otherwise, MagneticBeads is automatically set to Model[Sample, "Mag-Bind Particles CNR (Mag-Bind Viral DNA/RNA Kit)"]
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
    Programmatic Pattern: (ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MagneticBeadVolume

    The volumetric amount of MagneticBeads that is added to the assay container prior to optional prewash and equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/10 of the maximum Volume across the batch if ProcessingOrder->Batch or across all SamplesIn if ProcessingOrder->Parallel.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters.
    Programmatic Pattern: RangeP[1*Microliter, 50*Milliliter] | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MagnetizationRack

    The magnetic rack used during magnetization that provides the magnetic force to attract the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Item, MagnetizationRack, "Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] for robotic preparation. For manual preparation, set to the rack that fits tube with capacity of holding the maximum possible volume of sample during the experiment. See Table 3.1 for what this is automatically set to for manual preparation.
    Pattern Description: An object of type or subtype Model[Container, Rack], Object[Container, Rack], Model[Item, MagnetizationRack], or Object[Item, MagnetizationRack] or a prepared sample.
    Programmatic Pattern: (ObjectP[{Model[Container, Rack], Object[Container, Rack], Model[Item, MagnetizationRack], Object[Item, MagnetizationRack]}] | _String) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SampleLabel

    The user defined word or phrase used to identify the sample that is subject to magnetic bead separation and is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS Sample #".
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SampleContainerLabel

    The user defined word or phrase used to identify the sample's container (i.e. plate or tube) that are being used in the experiment, and is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS SampleContainer #".
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SampleOutLabel

    The user defined word or phrase used to identify the collected sample during loading or elution, depending on the selection strategy, and is used for identification elsewhere in sample preparation. The SampleOutLabel is used to identify the sample collected during elution if SelectionStrategy->Positive, or to identify the sample collected during loading if SelectionStrategy->Negative.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS Sample Out #".
    Pattern Description: A string or list of one or more a string or Null entries.
    Programmatic Pattern: ({(_String | Null)..} | _String) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ContainerOutLabel

    The user defined word or phrase used to identify the container of the output sample that will be created in this experiment, and is used for identification elsewhere in sample preparation.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. When ContainerOutLabel is fully Automatic, ContainerOutLabel is set to ElutionCollectionContainerLabel if SelectionStrategy->Positive, and is set to LoadingCollectionContainerLabel if SelectionStrategy->Negative. Otherwise, the unspecified member ContainerOutLabel is set to "MBS Container Out #".
    Pattern Description: A string or list of one or more a string or Null entries.
    Programmatic Pattern: ({(_String | Null)..} | _String) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashCollectionContainerLabel

    The user defined word or phrase used to identify the container for collecting the aspirated sample during prewash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationCollectionContainerLabel

    The user defined word or phrase used to identify the container for collecting the aspirated sample during equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingCollectionContainerLabel

    The user defined word or phrase used to identify the container for collecting the aspirated sample during loading.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the wash(es) prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the secondary wash(es) prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the tertiary wash(es) prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the quaternary wash(es) prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the quainary wash(es) prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the senary wash(es) prior to elution or optional SeptenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during the septenary wash(es) prior to elution.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionCollectionContainerLabel

    The user defined word or phrase used to identify the container used for collecting the aspirated sample(s) during elution(s).
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same container has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "MBS collection container #".
    Pattern Description: A string or list of one or more a string or Null entries or Null.
    Programmatic Pattern: ((Null | ({(_String | Null)..} | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SelectionStrategy

    Specified if the target analyte (Positive) or contaminants (Negative) binds to the magnetic beads in order to isolate the target analyte. When the target analyte is bound to the magnetic beads (Positive), they are collected as SamplesOut during the elution step. When contaminants are bound to the magnetic beads (Negative), the target analyte remains in the supernatant and is collected as SamplesOut during the loading step.
    Default Value: Positive
    Pattern Description: Positive or Negative.
    Programmatic Pattern: MagneticBeadSeparationSelectionStrategyP

    SeparationMode

    The mechanism used to selectively isolate or remove targeted components from the samples by magnetic beads. Options include NormalPhase, ReversePhase, IonExchange, Affinity. In NormalPhase mode, magnetic beads are coated with polar molecules (mainly pure silica) and the mobile phase less polar causing the adsorption of polar targeted components. In ReversePhase mode, magnetic beads are coated with hydrophobic groups on the surface to bind targeted components. In IonExchange mode, magnetic beads coated with ion-exchange groups ionically bind charged targeted components. In Affinity mode, magnetic beads are coated with functional groups that can covalently conjugate ligands on targeted components.
    Default Value: Affinity
    Pattern Description: NormalPhase, ReversePhase, IonExchange, or Affinity.
    Programmatic Pattern: MagneticBeadSeparationModeP

    ProcessingOrder

    The order for processing samples in the experiment. Parallel indicates all samples are processed at the same time in all steps of the experiment, can only be used if the samples are provided in a flat list. Serial indicates samples are processed sequentially such that all steps of the experiment are completed for a given sample before processing the next, can only be used if the samples are provided in a flat list. Batch indicates the input is a nested list and each sample group is fully processed (in parallel) before moving to the next, can only be used if the samples are provided in a nested list.
    Default Value: Automatic
    Default Calculation: Automatically set to Parallel if SamplesIn is a list of samples and to Batch if SamplesIn is a list of lists of samples.
    Pattern Description: Parallel, Batch, or Serial.
    Programmatic Pattern: MagneticBeadSeparationProcessingOrderP | Automatic

    NumberOfReplicates

    The number of aliquots to generate from the input sample before loading to the magnetic beads.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 and less than or equal to 500 in increments of 1 or Null.
    Programmatic Pattern: RangeP[2, 500, 1] | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    Post Experiment

    MagneticBeadCollectionStorageCondition

    The non-default condition under which the used magnetic beads are stored after the protocol is completed.
    Default Value: Disposal
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the prewash prior to equilibration are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if PreWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationCollectionStorageCondition

    The non-default condition under which the aspirated samples during the equilibration are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if Equilibration is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingCollectionStorageCondition

    The non-default condition under which the aspirated samples during loading are stored after the protocol is completed.
    Default Value: Refrigerator
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the wash prior to elution or optional SecondaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if Wash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the secondary wash prior to elution or optional TertiaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if SecondaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the tertiary wash prior to elution or optional QuaternaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if TertiaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the quaternary wash prior to elution or optional QuinaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if QuaternaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the quinary wash prior to elution or optional SenaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if QuinaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the senary wash prior to elution or optional SeptenaryWash are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if SenaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashCollectionStorageCondition

    The non-default condition under which the aspirated samples during the septenary wash prior to elution are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if SeptenaryWash is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionCollectionStorageCondition

    The non-default condition under which the aspirated samples during elution are stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: Automatically set to Refrigerator if Elution is set to True.
    Pattern Description: Condition or Objects or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    PreWash

    PreWash

    Indicates if the magnetic beads are rinsed prior to equilibration in order to remove the storage buffer.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other PreWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashBuffer

    The solution used to rinse the magnetic beads in order to remove the storage buffer prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to match the ElutionBuffer if PreWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashBufferVolume

    The amount of PreWashBuffer that is added to the magnetic beads for each prewash prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to the same as sample volume to load (Volume) if PreWash is set to True.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMix

    Indicates if the solution is mixed following combination of PreWashBuffer and the magnetic beads during each prewash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if PreWash is set to True and PreWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixType

    The style of motion used to mix the suspension following the addition of the PreWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined PreWashBuffer and magnetic beads) and the PreWashMix options. Specifically, PreWashMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of PreWashMixRate, PreWashMixTemperature, and PreWashMixTime is specified to non-default values; otherwise, PreWashMixType is automatically set to Vortex when Preparation is Manual, PreWashMixTemperature is Automatic or Ambient, and PreWashMixRate is specified to be greater than 600 RPM; otherwise, PreWashMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixTime

    The duration during which the combined PreWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If PreWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, PreWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixRate

    The frequency of rotation used to mix the combined PreWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if PreWashMixType is Shake or Stir, to 1000 RPM if PreWashMixType is Vortex or Disrupt, and to 30 RPM if PreWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfPreWashMixes

    The number of times that the combined PreWashBuffer and magnetic beads are mixed if PreWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if PreWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixVolume

    The volume of the combined PreWashBuffer and magnetic beads that is pipetted up and down in order to mix, if PreWashMixType->Pipette, .
    Default Value: Automatic
    Default Calculation: For robotic preparation, PreWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined PreWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, PreWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined PreWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if PreWashMix is set to True and PreWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixTipType

    The type of pipette tips used to mix the combined PreWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if PreWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, PreWashMixTipType is automatically set to WideBore if PreWashMixVolume is less or equal to 970 Microliter, to Normal if PreWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if PreWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the PreWashMix. This option can only be set if PreWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if PreWashMixVolume is less or equal to 5 Milliliter, and to Glass if PreWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashMagnetizationTime

    The duration of magnetizing the magnetic beads after PreWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used PreWashBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if PreWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each wash prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to PreWashBufferVolume for robotic preparation, if PreWash is set to True.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashAspirationPosition

    The location from which the solution is aspirated after PreWashMagnetizationTime. Top will aspirate PreWashAspirationPositionOffset below the Top of the container, Bottom will aspirate PreWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate PreWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation is Robotic and PreWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after PreWashMagnetizationTime. The Z Offset is based on the PreWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and PreWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashCollectionContainer

    The container(s) for collecting the aspirated used PreWashBuffer during the prewash(es) prior to equilibration. By default, the same container is selected for the repeated washes (i.e. aspirated samples in the repeated washes will be combined) unless different container objects are specified for the prewashes prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of PreWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if PreWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfPreWashes

    The number of times the magnetic beads are washed by adding PreWashBuffer, mixing, magnetization, and aspirating solution prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if PreWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining PreWashBuffer following the final prewash prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to False if PreWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PreWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining PreWashBuffer following the final prewash prior to equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if PreWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Equilibration

    Equilibration

    Indicates if the magnetic beads are equilibrated to a condition for optimal bead-target binding prior to adding samples to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other Equilibration options are set.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationBuffer

    The solution used for equilibrating the magnetic beads to a condition for optimal bead-target binding prior to adding samples to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if Equilibration is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationBufferVolume

    The amount of EquilibrationBuffer to add to the magnetic beads for equilibration.
    Default Value: Automatic
    Default Calculation: If Equilibration is set to True, EquilibrationBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMix

    Indicates if the solution is mixed following combination of EquilibrationBuffer and the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Equilibration is set to True and Equilibration MixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixType

    The style of motion used to mix the suspension following the addition of the EquilibrationBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined EquilibrationBuffer and magnetic beads) and the EquilibrationMix options. Specifically, EquilibrationMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of EquilibrationMixRate, EquilibrationMixTemperature, and EquilibrationMixTime is specified to non-default values; otherwise, EquilibrationMixType is automatically set to Vortex when Preparation is Manual, EquilibrationMixTemperature is Automatic or Ambient, and EquilibrationMixRate is specified to be greater than 600 RPM; otherwise, EquilibrationMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixTime

    The duration during which the combined EquilibrationBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If EquilibrationMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, EquilibrationMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixRate

    The frequency of rotation used to mix the combined EquilibrationBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if EquilibrationMixType is Shake or Stir, to 1000 RPM if EquilibrationMixType is Vortex or Disrupt, and to 30 RPM if EquilibrationMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfEquilibrationMixes

    The number of times that the combined EquilibrationBuffer and magnetic beads are mixed if EquilibrationMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if EquilibrationMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixVolume

    The volume of the combined EquilibrationBuffer and magnetic beads that is pipetted up and down in order to mix, if EquilibrationMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, EquilibrationMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined EquilibrationBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, EquilibrationMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixTemperature

    The temperature of the device that is used to mix/incubate the combined EquilibrationBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if EquilibrationMix is set to True and EquilibrationMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixTipType

    The type of pipette tips used to mix the combined EquilibrationBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if EquilibrationMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, EquilibrationMixTipType is automatically set to WideBore if EquilibrationMixVolume is less or equal to 970 Microliter, to Normal if EquilibrationMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if EquilibrationMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the EquilibrationMix. This option can only be set if EquilibrationMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if EquilibrationMixVolume is less or equal to 5 Milliliter, and to Glass if EquilibrationMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationMagnetizationTime

    The duration of magnetizing the magnetic beads after EquilibrationMixTime has elapsed in order to attract the magnetic beads to the side of the container by applying a magnetic force, thus enables removal or collection of the used EquilibrationBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if Equilibration is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to EquilibrationBufferVolume for robotic preparation, if Equilibration is set to True. Set to Null if Equilibration is set to False.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationAspirationPosition

    The location from which the solution is aspirated after EquilibrationMagnetizationTime. Top will aspirate EquilibrationAspirationPositionOffset below the Top of the container, Bottom will aspirate EquilibrationAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate EquilibrationAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation is Robotic and Equilibration is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after EquilibrationMagnetizationTime. The Z Offset is based on the EquilibrationAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and EquilibrationAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationCollectionContainer

    The container for collecting the aspirated used EquilibrationBuffer during the equilibration.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of EquilibrationAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if Equilibration is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining EquilibrationBuffer before contact with the input sample.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Equilibration is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    EquilibrationAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining EquilibrationBuffer before contact with the input sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if EquilibrationAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Loading

    LoadingMix

    Indicates if the solution is mixed following the combination of the input sample and the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to True if LoadingMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixType

    The style of motion used to mix the suspension following the addition of the SamplesIn to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined SamplesIn and magnetic beads) and the LoadingMix options. Specifically, LoadingMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of LoadingMixRate, LoadingMixTemperature, and LoadingMixTime is specified to non-default values; otherwise, LoadingMixType is automatically set to Vortex when Preparation is Manual, LoadingMixTemperature is Automatic or Ambient, and LoadingMixRate is specified to be greater than 600 RPM; otherwise, LoadingMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixTime

    The duration during which the combined SamplesIn and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If LoadingMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, LoadingMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixRate

    The frequency of rotation used to mix the combined SamplesIn and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if LoadingMixType is Shake or Stir, to 1000 RPM if LoadingMixType is Vortex or Disrupt, and to 30 RPM if LoadingMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfLoadingMixes

    The number of times that the combined SamplesIn and magnetic beads are mixed if LoadingMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if LoadingMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixVolume

    The volume of the combined SamplesIn and magnetic beads that is pipetted up and down in order to mix, if LoadingMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, LoadingMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the combined sample volume and magnetic beads volume) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, LoadingMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixTemperature

    The temperature of the device that is used to mix/incubate the combined SamplesIn and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if LoadingMix is set to True and LoadingMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixTipType

    The type of pipette tips used to mix the combined sample and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if LoadingMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, LoadingMixTipType is automatically set to WideBore if LoadingMixVolume is less or equal to 970 Microliter, to Normal if LoadingMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if LoadingMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the LoadingMix. This option can only be set if LoadingMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if LoadingMixVolume is less or equal to 5 Milliliter, and to Glass if LoadingMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingMagnetizationTime

    The duration of magnetizing the magnetic beads after LoadingMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the sample solution containing components that are not bound to the magnetic beads.
    Default Value: 5 minutes
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours.
    Programmatic Pattern: RangeP[0*Second, $MaxExperimentTime]
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingAspirationVolume

    The volume of sample solution to aspirate out while the magnetic beads are magnetized and gathered to the side.
    Default Value: Automatic
    Pattern Description: All or Volume.
    Programmatic Pattern: (All | RangeP[1*Microliter, 50*Milliliter]) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingAspirationPosition

    The location from which the solution is aspirated after LoadingMagnetizationTime. Top will aspirate LoadingAspirationPositionOffset below the Top of the container, Bottom will aspirate LoadingAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate LoadingAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after LoadingMagnetizationTime. The Z Offset is based on the LoadingAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and LoadingAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingCollectionContainer

    The container for collecting the aspirated sample during loading. By default, the same container is selected for samples in the same pool (i.e. aspirated samples in the same pool will be combined) unless different container objects are specified for samples in the pool.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of LoadingAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if Loading is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries.
    Programmatic Pattern: ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}})) | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining solution of the sample after aspirating the supernatant.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP
    Index Matches to: Sample
    Nested Index Matches to: Sample

    LoadingAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining solution of the sample after aspirating the supernatant.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if LoadingAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Wash

    Wash

    Indicates if the magnetic beads with bound targets or contaminants are rinsed prior to elution or optional SecondaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other Wash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashBuffer

    The solution used to rinse the magnetic beads during Wash prior to elution or optional SecondaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if Wash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashBufferVolume

    The amount of WashBuffer that is added to the magnetic beads for each wash prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: If Wash is set to True, WashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMix

    Indicates if the solution is mixed following combination of WashBuffer and the magnetic beads during each wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Wash is set to True and WashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixType

    The style of motion used to mix the suspension following the addition of the WashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined WashBuffer and magnetic beads) and the WashMix options. Specifically, WashMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of WashMixRate, WashMixTemperature, and WashMixTime is specified to non-default values; otherwise, WashMixType is automatically set to Vortex when Preparation is Manual, WashMixTemperature is Automatic or Ambient, and WashMixRate is specified to be greater than 600 RPM; otherwise, WashMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixTime

    The duration during which the combined WashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If WashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, WashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixRate

    The frequency of rotation used to mix the combined WashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if WashMixType is Shake or Stir, to 1000 RPM if WashMixType is Vortex or Disrupt, and to 30 RPM if WashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfWashMixes

    The number of times that the combined WashBuffer and magnetic beads are mixed if WashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if WashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixVolume

    The volume of the combined WashBuffer and magnetic beads that is pipetted up and down in order to mix, if WashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, WashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined WashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, WashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixTemperature

    The temperature of the device that is used to mix/incubate the combined WashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if WashMix is set to True and WashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixTipType

    The type of pipette tips used to mix the combined WashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if WashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, WashMixTipType is automatically set to WideBore if WashMixVolume is less or equal to 970 Microliter, to Normal if WashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if WashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the WashMix. This option can only be set if WashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if WashMixVolume is less or equal to 5 Milliliter, and to Glass if WashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashMagnetizationTime

    The duration of magnetizing the magnetic beads after WashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used WashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if Wash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each wash prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to WashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashAspirationPosition

    The location from which the solution is aspirated after WashMagnetizationTime. Top will aspirate WashAspirationPositionOffset below the Top of the container, Bottom will aspirate WashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate WashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and Wash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after WashMagnetizationTime. The Z Offset is based on the WashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and WashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the wash(es) prior to elution or optional SecondaryWash. By default, the same container is selected for the repeated washes (i.e. aspirated samples in the repeated washes will be combined) unless different container objects are specified for the washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of WashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if Wash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfWashes

    The number of times the magnetic beads are washed by adding WashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if Wash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining WashBuffer following the final wash prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if Wash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    WashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining WashBuffer following the final wash prior to elution or optional SecondaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if WashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWash

    Indicates if the magnetic beads with bound targets or contaminants are further rinsed after Wash prior to elution or optional TertiaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other SecondaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashBuffer

    The solution used to rinse the magnetic beads during SecondaryWash prior to elution or optional TertiaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if SecondaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashBufferVolume

    The amount of SecondaryWashBuffer that is added to the magnetic beads for each secondary wash prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: If SecondaryWash is set to True, SecondaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMix

    Indicates if the solution is mixed following combination of SecondaryWashBuffer and the magnetic beads during each secondary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if SecondaryWash is set to True and SecondaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixType

    The style of motion used to mix the suspension following the addition of the SecondaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined SecondaryWashBuffer and magnetic beads) and the SecondaryWashMix options. Specifically, SecondaryWashMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of SecondaryWashMixRate, SecondaryWashMixTemperature, and SecondaryWashMixTime is specified to non-default values; otherwise, SecondaryWashMixType is automatically set to Vortex when Preparation is Manual, SecondaryWashMixTemperature is Automatic or Ambient, and SecondaryWashMixRate is specified to be greater than 600 RPM; otherwise, SecondaryWashMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixTime

    The duration during which the combined SecondaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If SecondaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, SecondaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixRate

    The frequency of rotation used to mix the combined SecondaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if SecondaryWashMixType is Shake or Stir, to 1000 RPM if SecondaryWashMixType is Vortex or Disrupt, and to 30 RPM if SecondaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSecondaryWashMixes

    The number of times that the combined SecondaryWashBuffer and magnetic beads are mixed if SecondaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if SecondaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixVolume

    The volume of the combined SecondaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if SecondaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, SecondaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined SecondaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, SecondaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined SecondaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if SecondaryWashMix is set to True and SecondaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixTipType

    The type of pipette tips used to mix the combined SecondaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if SecondaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, SecondaryWashMixTipType is automatically set to WideBore if SecondaryWashMixVolume is less or equal to 970 Microliter, to Normal if SecondaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if SecondaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the SecondaryWashMix. This option can only be set if SecondaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if SecondaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if SecondaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after SecondaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used SecondaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if SecondaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each secondary wash prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to SecondaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashAspirationPosition

    The location from which the solution is aspirated after SecondaryWashMagnetizationTime. Top will aspirate SecondaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate SecondaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate SecondaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and SecondaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after SecondaryWashMagnetizationTime. The Z Offset is based on the SecondaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and SecondaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the secondary wash(es) prior to elution or optional TertiaryWash. By default, the same container is selected for the repeated secondary washes (i.e. aspirated samples in the repeated secondary washes will be combined) unless different container objects are specified for the secondary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of SecondaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if SecondaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSecondaryWashes

    The number of times the magnetic beads are washed by adding SecondaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if SecondaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining SecondaryWashBuffer following the final secondary wash prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if SecondaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SecondaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining SecondaryWashBuffer following the final secondary wash prior to elution or optional TertiaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if SecondaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWash

    Indicates if the magnetic beads with bound targets or contaminants are further rinsed after SecondaryWash prior to Elution or optional QuaternaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other TertiaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashBuffer

    The solution used to rinse the magnetic beads during TertiaryWash prior to elution or optional QuaternaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if TertiaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashBufferVolume

    The amount of TertiaryWashBuffer that is added to the magnetic beads for each tertiary wash prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: If TertiaryWash is set to True, TertiaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMix

    Indicates if the solution is mixed following combination of TertiaryWashBuffer and the magnetic beads during each tertiary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if TertiaryWash is set to True and TertiaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixType

    The style of motion used to mix the suspension following the addition of the TertiaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined TertiaryWashBuffer and magnetic beads) and the TertiaryWashMix options. Specifically, TertiaryWashMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of TertiaryWashMixRate, TertiaryWashMixTemperature, and TertiaryWashMixTime is specified to non-default values; otherwise, TertiaryWashMixType is automatically set to Vortex when Preparation is Manual, TertiaryWashMixTemperature is Automatic or Ambient, and TertiaryWashMixRate is specified to be greater than 600 RPM; otherwise, TertiaryWashMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixTime

    The duration during which the combined TertiaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If TertiaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, TertiaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixRate

    The frequency of rotation used to mix the combined TertiaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if TertiaryWashMixType is Shake or Stir, to 1000 RPM if TertiaryWashMixType is Vortex or Disrupt, and to 30 RPM if TertiaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfTertiaryWashMixes

    The number of times that the combined TertiaryWashBuffer and magnetic beads are mixed if TertiaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if TertiaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixVolume

    The volume of the combined TertiaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if TertiaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, TertiaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined TertiaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, TertiaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined TertiaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if TertiaryWashMix is set to True and TertiaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixTipType

    The type of pipette tips used to mix the combined TertiaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if TertiaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, TertiaryWashMixTipType is automatically set to WideBore if TertiaryWashMixVolume is less or equal to 970 Microliter, to Normal if TertiaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if TertiaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the TertiaryWashMix. This option can only be set if TertiaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if TertiaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if TertiaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after TertiaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used TertiaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if TertiaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each tertiary wash prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to TertiaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashAspirationPosition

    The location from which the solution is aspirated after TertiaryWashMagnetizationTime. Top will aspirate TertiaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate TertiaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate TertiaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and TertiaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after TertiaryWashMagnetizationTime. The Z Offset is based on the TertiaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and TertiaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the Tertiary wash(es) prior to elution or optional QuaternaryWash. By default, the same container is selected for the repeated tertiary washes (i.e. aspirated samples in the repeated tertiary washes will be combined) unless different container objects are specified for the tertiary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of TertiaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if TertiaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfTertiaryWashes

    The number of times the magnetic beads are washed by adding TertiaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if TertiaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining TertiaryWashBuffer following the final tertiary wash prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if TertiaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TertiaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining TertiaryWashBuffer following the final tertiary wash prior to elution or optional QuaternaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if TertiaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWash

    Indicates if the magnetic beads with bound targets or contaminants are further rinsed after TertiaryWash prior to Elution or optional QuinaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other QuaternaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashBuffer

    The solution used to rinse the magnetic beads during QuaternaryWash prior to elution or optional QuinaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if QuaternaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashBufferVolume

    The amount of QuaternaryWashBuffer that is added to the magnetic beads for each quaternary wash prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: If QuaternaryWash is set to True, QuaternaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMix

    Indicates if the solution is mixed following combination of QuaternaryWashBuffer and the magnetic beads during each quaternary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if QuaternaryWash is set to True and QuaternaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixType

    The style of motion used to mix the suspension following the addition of the QuaternaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the container of the sample and the QuaternaryWashMix option. Specifically, if QuaternaryWashMix is set to False, the option is set to Null. If any of QuaternaryWashMixRate, QuaternaryWashMixTemperature, and QuaternaryWashMixTime is specified to a non-default value, QuaternaryWashMixType is automatically set to Shake when Preparation is Robotic, when QuaternaryWashMixTemperature is not Ambient, or when QuaternaryWashMixRate is less or equal to 600 RPM; Otherwise QuaternaryWashMixType is automatically set to Vortex. If none of QuaternaryWashMixRate, QuaternaryWashMixTemperature, and QuaternaryWashMixTime is specified to non-default values, QuaternaryWashMixType is automatically set to Pipette.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixTime

    The duration during which the combined QuaternaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If QuaternaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, QuaternaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixRate

    The frequency of rotation used to mix the combined QuaternaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if QuaternaryWashMixType is Shake or Stir, to 1000 RPM if QuaternaryWashMixType is Vortex or Disrupt, and to 30 RPM if QuaternaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfQuaternaryWashMixes

    The number of times that the combined QuaternaryWashBuffer and magnetic beads are mixed if QuaternaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if QuaternaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixVolume

    The volume of the combined QuaternaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if QuaternaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, QuaternaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined QuaternaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, QuaternaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined QuaternaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if QuaternaryWashMix is set to True and QuaternaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixTipType

    The type of pipette tips used to mix the combined QuaternaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if QuaternaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, QuaternaryWashMixTipType is automatically set to WideBore if QuaternaryWashMixVolume is less or equal to 970 Microliter, to Normal if QuaternaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if QuaternaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the QuaternaryWashMix. This option can only be set if QuaternaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if QuaternaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if QuaternaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after QuaternaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used QuaternaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if QuaternaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each quaternary wash prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to QuaternaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashAspirationPosition

    The location from which the solution is aspirated after QuaternaryWashMagnetizationTime. Top will aspirate QuaternaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate QuaternaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate QuaternaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and QuaternaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after QuaternaryWashMagnetizationTime. The Z Offset is based on the QuaternaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and QuaternaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the Quaternary wash(es) prior to elution or optional QuinaryWash. By default, the same container is selected for the repeated quaternary washes (i.e. aspirated samples in the repeated quaternary washes will be combined) unless different container objects are specified for the quaternary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of QuaternaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if QuaternaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfQuaternaryWashes

    The number of times the magnetic beads are washed by adding QuaternaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if QuaternaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining QuaternaryWashBuffer following the final quaternary wash prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if QuaternaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuaternaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining QuaternaryWashBuffer following the final quaternary wash prior to elution or optional QuinaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if QuaternaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWash

    Indicates if the magnetic beads with bound targets or contaminants are further rinsed after QuaternaryWash prior to Elution or optional SenaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other QuinaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashBuffer

    The solution used to rinse the magnetic beads during QuinaryWash prior to elution or optional SenaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if QuinaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashBufferVolume

    The amount of QuinaryWashBuffer that is added to the magnetic beads for each quinary wash prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: If QuinaryWash is set to True, QuinaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMix

    Indicates if the solution is mixed following combination of QuinaryWashBuffer and the magnetic beads during each quinary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if QuinaryWash is set to True and QuinaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixType

    The style of motion used to mix the suspension following the addition of the QuinaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the container of the sample and the QuinaryWashMix option. Specifically, if QuinaryWashMix is set to False, the option is set to Null. If any of QuinaryWashMixRate, QuinaryWashMixTemperature, and QuinaryWashMixTime is specified to a non-default value, QuinaryWashMixType is automatically set to Shake when Preparation is Robotic, when QuinaryWashMixTemperature is not Ambient, or when QuinaryWashMixRate is less or equal to 600 RPM; Otherwise QuinaryWashMixType is automatically set to Vortex. If none of QuinaryWashMixRate, QuinaryWashMixTemperature, and QuinaryWashMixTime is specified to non-default values, QuinaryWashMixType is automatically set to Pipette.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixTime

    The duration during which the combined QuinaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If QuinaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, QuinaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixRate

    The frequency of rotation used to mix the combined QuinaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if QuinaryWashMixType is Shake or Stir, to 1000 RPM if QuinaryWashMixType is Vortex or Disrupt, and to 30 RPM if QuinaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfQuinaryWashMixes

    The number of times that the combined QuinaryWashBuffer and magnetic beads are mixed if QuinaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if QuinaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixVolume

    The volume of the combined QuinaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if QuinaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, QuinaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined QuinaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, QuinaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined QuinaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if QuinaryMix is set to True and QuinaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixTipType

    The type of pipette tips used to mix the combined QuinaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if QuinaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, QuinaryWashMixTipType is automatically set to WideBore if QuinaryWashMixVolume is less or equal to 970 Microliter, to Normal if QuinaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if QuinaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the QuinaryWashMix. This option can only be set if QuinaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if QuinaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if QuinaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after QuinaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used QuinaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if QuinaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each quinary wash prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to QuinaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashAspirationPosition

    The location from which the solution is aspirated after QuinaryWashMagnetizationTime. Top will aspirate QuinaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate QuinaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate QuinaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and QuinaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after QuinaryWashMagnetizationTime. The Z Offset is based on the QuinaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and QuinaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the Quinary wash(es) prior to elution or optional SenaryWash. By default, the same container is selected for the repeated quinary washes (i.e. aspirated samples in the repeated quinary washes will be combined) unless different container objects are specified for the quinary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of QuinaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if QuinaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfQuinaryWashes

    The number of times the magnetic beads are washed by adding QuinaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if QuinaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining QuinaryWashBuffer following the final quinary wash prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if QuinaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    QuinaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining QuinaryWashBuffer following the final quinary wash prior to elution or optional SenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if QuinaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWash

    Indicates if the magnetic beads with bound targets or contaminants are further rinsed after QuinaryWash prior to elution or optional SeptenaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other SenaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashBuffer

    The solution used to rinse the magnetic beads during SenaryWash prior to Elution or optional SeptenaryWash, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if SenaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashBufferVolume

    The amount of SenaryWashBuffer that is added to the magnetic beads for each senary wash prior to elution.
    Default Value: Automatic
    Default Calculation: If SenaryWash is set to True, SenaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMix

    Indicates if the solution is mixed following combination of SenaryWashBuffer and the magnetic beads during each senary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if SenaryWash is set to True and SenaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixType

    The style of motion used to mix the suspension following the addition of the SenaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the container of the sample and the SenaryWashMix option. Specifically, if SenaryWashMix is set to False, the option is set to Null. If any of SenaryWashMixRate, SenaryWashMixTemperature, and SenaryWashMixTime is specified to a non-default value, SenaryWashMixType is automatically set to Shake when Preparation is Robotic, when SenaryWashMixTemperature is not Ambient, or when SenaryWashMixRate is less or equal to 600 RPM; Otherwise SenaryWashMixType is automatically set to Vortex. If none of SenaryWashMixRate, SenaryWashMixTemperature, and SenaryWashMixTime is specified to non-default values, SenaryWashMixType is automatically set to Pipette.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixTime

    The duration during which the combined SenaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If SenaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, SenaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixRate

    The frequency of rotation used to mix the combined SenaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if SenaryWashMixType is Shake or Stir, to 1000 RPM if SenaryWashMixType is Vortex or Disrupt, and to 30 RPM if SenaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSenaryWashMixes

    The number of times that the combined SenaryWashBuffer and magnetic beads are mixed if SenaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if SenaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixVolume

    The volume of the combined SenaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if SenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, SenaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined SenaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, SenaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined SenaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if SenaryMix is set to True and SenaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixTipType

    The type of pipette tips used to mix the combined SenaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if SenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, SenaryWashMixTipType is automatically set to WideBore if SenaryWashMixVolume is less or equal to 970 Microliter, to Normal if SenaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if SenaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the SenaryWashMix. This option can only be set if SenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if SenaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if SenaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after SenaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used SenaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if SenaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each senary wash prior to elution or optional SeptenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to SenaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashAspirationPosition

    The location from which the solution is aspirated after SenaryWashMagnetizationTime. Top will aspirate SenaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate SenaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate SenaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and SenaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after SenaryWashMagnetizationTime. The Z Offset is based on the SenaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and SenaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the senary wash(es) prior to elution or optional SeptenaryWash. By default, the same container is selected for the repeated senary washes (i.e. aspirated samples in the repeated senary washes will be combined) unless different container objects are specified for the senary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of SenaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if SenaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSenaryWashes

    The number of times the magnetic beads are washed by adding SenaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution or optional SeptenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if SenaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining SenaryWashBuffer following the final senary wash prior to elution or optional SeptenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to False if SenaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SenaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining SenaryWashBuffer following the final senary wash prior to elution or optional SeptenaryWash.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if SenaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWash

    Indicates if the magnetic beads with bound targets or contaminants are rinsed after SenaryWash prior to Elution, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion. The washing steps (up to SeptenaryWash) can optionally be used for on-bead digestion to perform alkylation, reduction, trypsin digestion, etc.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other SeptenaryWash options are set, or False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashBuffer

    The solution used to rinse the magnetic beads during SeptenaryWash prior to elution, in order to better separate the bead-bound components from the unbound components in the sample, or for on-bead digestion.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if SeptenaryWash is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashBufferVolume

    The amount of SeptenaryWashBuffer that is added to the magnetic beads for each septenary wash prior to elution.
    Default Value: Automatic
    Default Calculation: If SeptenaryWash is set to True, SeptenaryWashBufferVolume is automatically set to PreWashBufferVolume when PreWash is set to True, and is set to Volume when PreWash is set to False.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMix

    Indicates if the solution is mixed following combination of SeptenaryWashBuffer and the magnetic beads during each septenary wash.
    Default Value: Automatic
    Default Calculation: Automatically set to True if SeptenaryWash is set to True and SeptenaryWashMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixType

    The style of motion used to mix the suspension following the addition of the SeptenaryWashBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the container of the sample and the SeptenaryWashMix option. Specifically, if SeptenaryWashMix is set to False, the option is set to Null. If any of SeptenaryWashMixRate, SeptenaryWashMixTemperature, and SeptenaryWashMixTime is specified to a non-default value, SeptenaryWashMixType is automatically set to Shake when Preparation is Robotic, when SeptenaryWashMixTemperature is not Ambient, or when SeptenaryWashMixRate is less or equal to 600 RPM; Otherwise SeptenaryWashMixType is automatically set to Vortex. If none of SeptenaryWashMixRate, SeptenaryWashMixTemperature, and SeptenaryWashMixTime is specified to non-default values, SeptenaryWashMixType is automatically set to Pipette.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixTime

    The duration during which the combined SeptenaryWashBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If SeptenaryWashMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, SeptenaryWashMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixRate

    The frequency of rotation used to mix the combined SeptenaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if SeptenaryWashMixType is Shake or Stir, to 1000 RPM if SeptenaryWashMixType is Vortex or Disrupt, and to 30 RPM if SeptenaryWashMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSeptenaryWashMixes

    The number of times that the combined SeptenaryWashBuffer and magnetic beads are mixed if SeptenaryWashMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if SeptenaryWashMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixVolume

    The volume of the combined SeptenaryWashBuffer and magnetic beads that is pipetted up and down in order to mix, if SeptenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, SeptenaryWashMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined SeptenaryWashBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, SeptenaryWashMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixTemperature

    The temperature of the device that is used to mix/incubate the combined SeptenaryWashBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if SeptenaryMix is set to True and SeptenaryWashMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixTipType

    The type of pipette tips used to mix the combined SeptenaryWashBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if SeptenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, SeptenaryWashMixTipType is automatically set to WideBore if SeptenaryWashMixVolume is less or equal to 970 Microliter, to Normal if SeptenaryWashMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if SeptenaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the SeptenaryWashMix. This option can only be set if SeptenaryWashMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if SeptenaryWashMixVolume is less or equal to 5 Milliliter, and to Glass if SeptenaryWashMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashMagnetizationTime

    The duration of magnetizing the magnetic beads after SeptenaryWashMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the used SeptenaryWashBuffer containing residual sample components that are not bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if SeptenaryWash is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashAspirationVolume

    The volume of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each septenary wash prior to elution.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to SeptenaryWashBufferVolume for robotic preparation.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashAspirationPosition

    The location from which the solution is aspirated after SeptenaryWashMagnetizationTime. Top will aspirate SeptenaryWashAspirationPositionOffset below the Top of the container, Bottom will aspirate SeptenaryWashAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate SeptenaryWashAspirationPositionOffset below the liquid level of the sample in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and SeptenaryWash is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after SeptenaryWashMagnetizationTime. The Z Offset is based on the SeptenaryWashAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and SeptenaryWashAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashCollectionContainer

    The container(s) for collecting the aspirated sample(s) during the Septenary wash(es) prior to elution. By default, the same container is selected for the repeated septenary washes (i.e. aspirated samples in the repeated septenary washes will be combined) unless different container objects are specified for the septenary washes.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of SeptenaryWashAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if SeptenaryWash is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfSeptenaryWashes

    The number of times the magnetic beads are washed by adding SeptenaryWashBuffer, mixing, magnetization, and aspirating solution prior to elution.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if SeptenaryWash is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashAirDry

    Indicates if the magnetic beads are exposed to open air to evaporate the remaining SeptenaryWashBuffer following the final septenary wash prior to elution.
    Default Value: Automatic
    Default Calculation: Automatically set to False if SeptenaryWash is set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    SeptenaryWashAirDryTime

    The duration that the magnetic beads are exposed to open air to evaporate the remaining SeptenaryWashBuffer following the final septenary wash prior to elution.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if SeptenaryWashAirDry is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Elution

    Elution

    Indicates if the magnetic beads are rinsed in a different buffer condition in order to release the components bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to True if other Elution options are set or if SelectionStrategy->Positive, and is set to False otherwise.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionBuffer

    The solution used to rinse the magnetic beads, providing a buffer condition in order to release the components bound to the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if Elution is set to True.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionBufferVolume

    The amount of ElutionBuffer that is added to the magnetic beads for each elution.
    Default Value: Automatic
    Default Calculation: Automatically set to 1/10 of the Volume if Elution is set to True.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMix

    Indicates if the solution is mixed following combination of ElutionBuffer and the magnetic beads during each elution.
    Default Value: Automatic
    Default Calculation: Automatically set to True if Elution is set to True and ElutionMixType is not set to Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixType

    The style of motion used to mix the suspension following the addition of the ElutionBuffer to the magnetic beads. Options include Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, Nutate.
    Default Value: Automatic
    Default Calculation: Automatically set based on the volume-to-mix (i.e. the volume of the combined ElutionBuffer and magnetic beads) and the ElutionMix options. Specifically, ElutionMixType is automatically set to Pipette when the volume-to-mix is greater than 20 Microliter and none of ElutionMixRate, ElutionMixTemperature, and ElutionMixTime is specified to non-default values; otherwise, ElutionMixType is automatically set to Vortex when Preparation is Manual, ElutionMixTemperature is Automatic or Ambient, and ElutionMixRate is specified to be greater than 600 RPM; otherwise, ElutionMixType is automatically set to Shake.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixTime

    The duration during which the combined ElutionBuffer and magnetic beads are mixed.
    Default Value: Automatic
    Default Calculation: If ElutionMixType is among Disrupt, Nutate, Shake, Vortex, Roll, Stir, Homogenize, and Sonicate, ElutionMixTime is automatically set to 5 Minute for robotic preparation, and is set to 15 Minute for manual preparation.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixRate

    The frequency of rotation used to mix the combined ElutionBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if ElutionMixType is Shake or Stir, to 1000 RPM if ElutionMixType is Vortex or Disrupt, and to 30 RPM if ElutionMixType is Nutate or Roll.
    Pattern Description: Gravitational Acceleration (Acoustic Shaker Only) or RPM or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinMixRate, $MaxMixRate] | RangeP[0*GravitationalAcceleration, 100*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfElutionMixes

    The number of times that the combined ElutionBuffer and magnetic beads are mixed if ElutionMixType is Pipette or Invert.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 if ElutionMixType is Pipette, Swirl or Invert.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 50, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixVolume

    The volume of the combined ElutionBuffer and magnetic beads that is pipetted up and down in order to mix, if ElutionMixType->Pipette.
    Default Value: Automatic
    Default Calculation: For robotic preparation, ElutionMixVolume is automatically set to 970 Microliter if 0.8*volume-to-mix (i.e.volume-to-mix is the volume of the combined ElutionBuffer and magnetic beads) is greater than 970 Microliter, and otherwise is set to the greater of 10 Microliter and 0.8*volume-to-mix. For manual preparation, ElutionMixVolume is automatically set to the lesser of 50 Milliliter and 0.8*volume-to-mix.
    Pattern Description: Greater than or equal to 0.5 microliters and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0.5*Microliter, 50*Milliliter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixTemperature

    The temperature of the device that is used to mix/incubate the combined ElutionBuffer and magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if ElutionMix is set to True and ElutionMixType is not Null.
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (RangeP[$MinIncubationTemperature, $MaxIncubationTemperature] | Ambient)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixTipType

    The type of pipette tips used to mix the combined ElutionBuffer and magnetic beads. Options include Normal, Barrier, WideBore, GelLoading, Aspirator. This option can only be set if ElutionMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to WideBore for robotic preparation. For manual preparation, ElutionMixTipType is automatically set to WideBore if ElutionMixVolume is less or equal to 970 Microliter, to Normal if ElutionMixVolume is larger than 970 Microliter but less or equal to 5 Milliliter, and to Barrier if ElutionMixVolume is larger than 5 Milliliter.
    Pattern Description: Normal, Barrier, WideBore, GelLoading, or Aspirator or Null.
    Programmatic Pattern: ((Null | TipTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMixTipMaterial

    The material of the pipette tips used to aspirate and dispense the requested volume during the ElutionMix. This option can only be set if ElutionMixType->Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to Polypropylene if ElutionMixVolume is less or equal to 5 Milliliter, and to Glass if ElutionMixVolume is larger than 5 Milliliter.
    Pattern Description: {ABS, PLA, Acrylic, AmorphousFluoropolymer, CPVC, CTFE, Cycloolefine, COC, Delrin, ECTFE, EPDM, ETFE, EVA, FEP, FFKM, HDPE, Hypalon, LDPE, NaturalRubber, NBR, Neoprene, Nitrile, Noryl, Nylon, PEEK, PEI, Perlast, PharmaPure, Polycarbonate, Polyester, Polyethylene, Polyisoprene, Polyolefin, Polyoxymethylene, Polypropylene, Polystyrene, Polyurethane, PVC, PCTFE, PETG, PF, PFA, PPS, PTFE, PVDF, SEBS, Silicone, SyntheticRubber, TFM, TPE, Tygon, UVPlastic, UVXPO, Viton}, {Aluminum, Alloy, AnodisedAluminum, Brass, Bronze, CarbonSteel, CastIron, Chrome, Copper, Elgiloy, Gold, Hastelloy, Lead, Magnesium, Molybdenum, Nickel, Niobium, Platinum, Silver, Steel, StainlessSteel, Titanium, Tungsten, Zinc}, {Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, HLB}, {Silica, Alumina, ResinParticlesWithLatexMicroBeads, CrossLinkedDextranBeads, CrossLinkedPolystyrene, AerisCoreShell, KinetexCoreShell, CrossLinkedAgarose, Vydac218MS, JordiGel, Styrene, SilicaCompositeTWIN, BEH, CSH, HSS, CarboPacPA1, CarboPacPA10, BEH}, Polysulfone, Agate, AluminiumOxide, ZirconiumOxide, Cardboard, Ceramic, Epoxy, EpoxyResin, BorosilicateGlass, Glass, GlassyCarbon, Graphite, OpticalGlass, Porcelain, Quartz, UVQuartz, ESQuartz, FusedQuartz, IRQuartz, Oxidizer, Ruby, Sapphire, Silicon, Silver, Viton, Styrofoam, {VacuumMeltedStainlessSteel}, or Wood or Null.
    Programmatic Pattern: ((Null | MaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionMagnetizationTime

    The duration of magnetizing the magnetic beads after ElutionMixTime has elapsed, in order to pull the magnetic beads to the perimeter of the container by applying a magnetic force, thus enables maximal aspiration of the ElutionBuffer containing sample components released from the magnetic beads.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 minutes if Elution is set to True.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionAspirationVolume

    The amount of solution to aspirate out while the magnetic beads are magnetized and gathered to the side during each elution.
    Default Value: Automatic
    Default Calculation: Automatically set to All for manual preparation, and set to ElutionBufferVolume for robotic preparation, if Elution is set to True. Set to Null if Elution is set to False.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (All | RangeP[1*Microliter, 50*Milliliter])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionAspirationPosition

    The location from which the solution is aspirated after ElutionMagnetizationTime. Top will aspirate ElutionAspirationPositionOffset below the Top of the container, Bottom will aspirate ElutionAspirationPositionOffset above the Bottom of the container, and LiquidLevel will aspirate ElutionAspirationPositionOffset below the liquid level of the sample in the container
    Default Value: Automatic
    Default Calculation: Automatically set to Bottom if Preparation->Robotic and Elution is True.
    Pattern Description: Top, Bottom, or LiquidLevel or Null.
    Programmatic Pattern: ((Null | MagneticBeadSeparationPipettingPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionAspirationPositionOffset

    The distance from the center of the well that the solution is aspirated after ElutionMagnetizationTime. The Z Offset is based on the ElutionAspirationPosition option -- measured as the height below the top of the well (Top), the height above the bottom of the well (Bottom), or the height below the detected liquid level (LiquidLevel). Please refer to the AspirationPosition diagram in the help file of ExperimentTransfer for more information. If an X and Y offset is not specified, the liquid will be aspirated in the center of the well, otherwise, -X/+X values will shift the position left and right, respectively, and -Y/+Y values will shift the position down and up, respectively.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 Millimeter if MagnetizationRack is Model[Item,MagnetizationRack,"Alpaqua Magnum FLX Enhanced Universal Magnet 96-well Plate Rack"] and ElutionAspirationPosition is bottom, otherwise set to 2 Millimeter.
    Pattern Description: {X,Y,Z} Coordinate Offset or Z Offset or Null.
    Programmatic Pattern: ((Null | (GreaterEqualP[0*Millimeter] | Coordinate[{DistanceP, DistanceP, GreaterEqualP[0*Millimeter]}])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ElutionCollectionContainer

    The container(s) for collecting the aspirated sample(s) during elution(s). By default, the same container is selected for the repeated elutions (i.e. aspirated samples in the repeated elutions will be combined) unless different container objects are specified for the elutions.
    Default Value: Automatic
    Default Calculation: Automatically set to a container plate and its first available position. The container plate is selected by the function PreferredContainer given the volume of ElutionAspirationVolume for manual preparation, and is set to 2 mL Deep well plate for robotic preparation, if Elution is set to True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or list of one or more Container or Container with Index or Container with Well or Container with Well and Index or Null entries or Null.
    Programmatic Pattern: ((Null | ({((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}} | Null)..} | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[Model[Container]]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}))) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    NumberOfElutions

    The number of times the bound components on the magnetic beads are eluted by adding ElutionBuffer, mixing, magnetization, and aspirating solution.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if Elution is set to True.
    Pattern Description: Greater than or equal to 1 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, 20, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: ((Null | MixTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[1*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: ((Null | ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[-10*Celsius, 40*Celsius])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: ((Null | FiltrationTypeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: ((Null | GreaterP[0*Minute]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Null | (Alternatives[GreaterEqualP[4*Celsius]])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((Null | (ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic})) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: ((Null | BooleanP) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AliquotSampleLabel

    A user defined word or phrase used to identify the samples after they are aliquotted, for use in downstream unit operations.
    Default Value: Automatic
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)])) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((Null | RangeP[1*Microliter, 20*Liter]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 in increments of 1 or Null.
    Programmatic Pattern: ((Null | GreaterEqualP[1, 1]) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Sample], Object[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((Null | (SampleStorageTypeP | Disposal)) | Automatic) | Null
    Index Matches to: Sample
    Nested Index Matches to: Sample

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or list of one or more any well from A1 to H12 or any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..} | {(((Automatic | Null) | WellPositionP) | {((Automatic | Null) | WellPositionP)..})..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries or list of one or more list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries entries or list of one or more list of one or more Automatic or Null or {Index, Container} entries entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..} | {{((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..}..} | {{({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

LiquidLiquidExtraction

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples that contain the target analyte to be isolated via liquid liquid extraction.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ObjectP[{Object[Sample], Object[Container]}] | _String
    Index Matches to: Sample

    ExtractionTechnique

    The method that is used to separate the aqueous and organic phase of a sample. The collection of the target phase occurs after the extraction solvent(s) and demulsifier (if specified) are added, the sample is mixed (optionally), allowed to settle for SettlingTime (for the organic and aqueous phases to separate), and centrifuged (optionally). Pipette uses a pipette to aspirate off either the aqueous or organic layer, optionally taking the boundary layer with it according to the IncludeBoundary and ExtractionBoundaryVolume options. PhaseSeparator uses a column with a hydrophobic frit, which allows the organic phase to pass freely through the frit, but physically blocks the aqueous phase from passing through. Note that when using a phase separator, the organic phase must be heavier than the aqueous phase in order for it to pass through the hydrophobic frit, otherwise, the separator will not occur.
    Default Value: Automatic
    Default Calculation: If the option ExtractionDevice is set, ExtractionTechnique is set to PhaseSeparator (this option only applies to the use of a phase separator). If the options IncludeBoundary or ExtractionBoundaryVolume are set, ExtractionTechnique is set to Pipette (these options only apply to the use of a pipette for separation). Otherwise, set to PhaseSeparator by default.
    Pattern Description: Pipette or PhaseSeparator.
    Programmatic Pattern: ExtractionTechniqueP | Automatic
    Index Matches to: Sample

    ExtractionDevice

    The device which is used to physically separate the aqueous and organic phases.
    Default Value: Automatic
    Default Calculation: If ExtractionTechnique is set to PhaseSeparator, ExtractionDevice will be set to Model[Container, Plate, PhaseSeparator, "Semi-Transparent Plastic 96 Fixed Well Plate with Phase Separator Frits"].
    Pattern Description: An object of type or subtype Model[Container, Plate, PhaseSeparator] or Object[Container, Plate, PhaseSeparator] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Plate, PhaseSeparator], Object[Container, Plate, PhaseSeparator]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    SelectionStrategy

    Indicates if additional rounds of extraction are performed on the impurity phase (Positive) or the TargetPhase (Negative). Positive selection is used when the goal is to extract the maximum amount of TargetAnalyte from the impurity phase (maximizing yield). Negative selection is used when the goal is to remove impurities that may still exist in the TargetPhase (maximizing purity).
    Default Value: Automatic
    Default Calculation: If NumberOfExtractions is set to 1, then set to Null (this option only applies if there are multiple rounds of extraction). If SolventAdditions or AqueousSolvent/OrganicSolvent is specified, then the solvent(s) specified are used to infer the SelectionStrategy (if the phase of the solvent being added matches TargetPhase, then SelectionStrategy is set to Positive (extraction is done on the impurity layer), otherwise SelectionStrategy is set to Negative). Otherwise, automatically set to Positive selection.
    Pattern Description: Positive or Negative or Null.
    Programmatic Pattern: ((Null | SelectionStrategyP) | Automatic) | Null
    Index Matches to: Sample

    IncludeBoundary

    Indicates if the boundary layer is aspirated along with the ExtractionTransferLayer. This option is only applicable when ExtractionTechnique is set to Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to False if ExtractionTechnique is set to Pipette. Otherwise, set to Null since IncludeBoundary does not apply when ExtractionTechnique is PhaseSeparator since the hydrophobic frit of the phase separator automatically will only allow the organic phase to pass through.
    Pattern Description: List of one or more True or False entries or Null.
    Programmatic Pattern: ((Null | {BooleanP..}) | Automatic) | Null
    Index Matches to: Sample

    TargetAnalyte

    The desired molecular entity that the extraction is designed to isolate.
    Default Value: Automatic
    Default Calculation: Automatically set to the value of Analytes field in the input sample, if the Analytes field is populated. Otherwise, set to the first element of the Composition field that's listed in terms of concentration (molarity) or mass concentration (grams/L). Otherwise, set to Null.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: ((Null | ObjectP[IdentityModelTypes]) | Automatic) | Null
    Index Matches to: Sample

    SamplePhase

    Indicates the phase of the input sample before extraction has taken place. Aqueous means that the input sample is liquid and composed only of aqueous solvents. Organic means that the input sample is liquid and composed only of organic solvents. Biphasic means that the input sample is liquid and composed of both aqueous and organic solvents that are separated into two defined layers. Unknown means that the sample phase is unknown, which will result in both Aqueous and Organic solvents being added to the input sample.
    Default Value: Automatic
    Default Calculation: Automatically set according to the PredictSamplePhase[...] function which looks at the composition of the sample's Solvent field to predict the sample's phase. If there is not enough information about the input sample for PredictSamplePhase[...] to predict the input sample's phase, a warning will be thrown. For more information, please refer to the PredictSamplePhase help file.
    Pattern Description: Aqueous, Organic, Biphasic, or Unknown.
    Programmatic Pattern: SamplePhaseP | Automatic
    Index Matches to: Sample

    TargetPhase

    Indicates the phase that is collected during the extraction (and carried forward to further experiments as defined by the SamplesOut field), which is the liquid layer that contains more of the dissolved TargetAnalyte after the SettlingTime has elapsed and the phases are separated.
    Default Value: Automatic
    Default Calculation: Automatically set to the phase (Organic or Aqueous) that the TargetAnalyte is more likely to be present in after extraction according to the PredictDestinationPhase[...] function. If there is not enough information for PredictDestinationPhase[...] to predict the destination phase of the target molecule, a warning will be thrown and TargetPhase will default to Aqueous. For more information, please refer to the PredictDestinationPhase help file.
    Pattern Description: Aqueous or Organic.
    Programmatic Pattern: TargetPhaseP | Automatic
    Index Matches to: Sample

    TargetLayer

    Indicates if the target phase is the top layer or the bottom layer of the separated solution. Note that when performing multiple rounds of extraction (NumberOfExtractions), the composition of the Aqueous and Organic layers during the first round of extraction can differ from the rest of the extraction rounds. For example, if SamplePhase->Biphasic, TargetPhase->Organic, and SelectionStrategy->Positive, the original organic layer from the input sample will be extracted and in subsequent rounds of extraction, OrganicSolvent added to the Aqueous impurity layer to extract more TargetAnalyte (the specified OrganicSolvent option can differ from the density of the original sample's organic layer). This can result in TargetLayer being different during the first round of extraction compared to the rest of the extraction rounds.
    Default Value: Automatic
    Default Calculation: Automatically calculated from the density of the input sample's aqueous and organic layers (if present in the input sample), the density of the AqueousSolvent and OrganicSolvent options (if specified), and the TargetPhase option. If density information is missing for any of these previously mentioned samples/layers, a warning will be thrown and it will be assumed that the Aqueous layer is on Top (less dense) than the Organic layer. During the calculation of this option, it is assumed that the additional molecules from the input sample will not significantly affect the densities of the aqueous and organic layers. It is also assumed that the Aqueous and Organic layers are fully separated after each round of extraction.
    Pattern Description: List of one or more Top or Bottom entries.
    Programmatic Pattern: {TargetLayerP..} | Automatic
    Index Matches to: Sample

    SampleLabel

    A user defined word or phrase used to identify the input sample, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid extraction sample #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    SampleContainerLabel

    A user defined word or phrase used to identify the input sample's container, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid extraction sample container #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    TargetLabel

    A user defined word or phrase used to identify the sample that contains the extracted target layer sample, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid target sample #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    TargetContainerLabel

    A user defined word or phrase used to identify the container that contains the extracted target layer sample, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid target sample container #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    ImpurityLabel

    A user defined word or phrase used to identify the sample that contains the waste layer, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid impurity sample #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    ImpurityContainerLabel

    A user defined word or phrase used to identify the container that contains the waste layer sample, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to the previously specified label if the same sample has already been labeled in an upstream unit operation within the same sample preparation protocol. Otherwise, set to "liquid liquid impurity sample container #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: Sample

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Robotic
    Pattern Description: Robotic.
    Programmatic Pattern: Robotic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Phase Mixing

    SampleVolume

    The volume of the input sample that is aliquotted into the ExtractionContainer and the liquid liquid extraction is performed on.
    Default Value: Automatic
    Default Calculation: Automatically set to either half the volume of the ExtractionContainer or the Volume of the input sample, which ever is smaller, if ExtractionContainer is specified. Otherwise, is set to Null.
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: ((Null | (RangeP[0*Microliter, $MaxRoboticTransferVolume] | All)) | Automatic) | Null
    Index Matches to: Sample

    ExtractionContainer

    The container that the input sample that is aliquotted into, before the liquid liquid extraction is performed.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] if SampleVolume is set and the input sample is not in a centrifuge compatible container and Centrifugation is specified or if a non-Ambient Temperature is specified (the robotic heater/cooler units are only compatible with Plate format containers). Otherwise, if SampleVolume is set, PreferredContainer[...] is used to get a robotic compatible container that can hold the sample volume specified. Otherwise, set to Null.
    Pattern Description: Existing Container or New Container or New Container with Index or Null.
    Programmatic Pattern: ((Null | ((ObjectP[Object[Container]] | _String) | ObjectP[Model[Container]] | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]})) | Automatic) | Null
    Index Matches to: Sample

    ExtractionContainerWell

    The well of the container that the input sample that is aliquotted into, before the liquid liquid extraction is performed.
    Default Value: Automatic
    Default Calculation: Automatically set to the first empty position in the ExtractionContainer, if specified. Otherwise, set to Null.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: ((Null | Alternatives @@ Flatten[AllWells[NumberOfWells -> 96]]) | Automatic) | Null
    Index Matches to: Sample

    AqueousSolvent

    The aqueous solvent that is added to the input sample (or the impurity layer from the previous extraction round if NumberOfExtractions > 1) in order to create an organic and aqueous phase.
    Default Value: Automatic
    Default Calculation: If Aqueous solvent is required for the extraction, Model[Sample, "Milli-Q water"] is used as the AqueousSolvent. Otherwise, set to None.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or None.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | None) | Automatic
    Index Matches to: Sample

    AqueousSolventVolume

    The volume of aqueous solvent that is added and mixed with the sample during each extraction.
    Default Value: Automatic
    Default Calculation: If AqueousSolventRatio is set, AqueousSolventVolume is calculated by multiplying 1/AqueousSolventRatio with the sample volume. Otherwise, if AqueousSolvent is set, set to 20% of the volume of the sample being extracted.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    AqueousSolventRatio

    The ratio of the sample volume to the volume of aqueous solvent that is added to the sample.
    Default Value: Automatic
    Default Calculation: If AqueousSolventVolume is set, AqueousSolventRatio is calculated by dividing the sample volume by AqueousSolventVolume. Otherwise, if AqueousSolvent is set, set to 5.
    Pattern Description: Greater than 0 or Null.
    Programmatic Pattern: ((Null | GreaterP[0]) | Automatic) | Null
    Index Matches to: Sample

    OrganicSolvent

    The organic solvent that is added to the input sample (or the impurity layer from the previous extraction round if NumberOfExtractions > 1) in order to create an organic and aqueous phase.
    Default Value: Automatic
    Default Calculation: If Organic solvent is required for the extraction and ExtractionTechnique is Pipette, Model[Sample, "Ethyl acetate, HPLC Grade"] is used as the OrganicSolvent. If Organic solvent is required for the extraction and ExtractionTechnique is PhaseSeparator, Model[Sample, "Ethyl acetate, HPLC Grade"] is used as the OrganicSolvent if it denser than the sample's Aqueous phase and the AqueousSolvent (if specified) since the phase separator will only be able to let the Organic layer pass through the hydrophobic frit if it is on the bottom. If Ethyl Acetate is not dense enough, Model[Sample, "Chloroform"] will be used. Otherwise, set to None.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or None.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | None) | Automatic
    Index Matches to: Sample

    OrganicSolventVolume

    The volume of organic solvent that is added and mixed with the sample during each extraction.
    Default Value: Automatic
    Default Calculation: If OrganicSolventRatio is set, OrganicSolventVolume is calculated by multiplying 1/OrganicSolventRatio with the sample volume. Otherwise, if OrganicSolvent is set, set to 20% of the volume of the sample being extracted.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    OrganicSolventRatio

    The ratio of the sample volume to the volume of orgnanic solvent that is added to the sample.
    Default Value: Automatic
    Default Calculation: If OrganicSolventVolume is set, OrganicSolventRatio is calculated by dividing the sample volume by OrganicSolventVolume. Otherwise, if OrganicSolvent is set, set to 5.
    Pattern Description: Greater than 0 or Null.
    Programmatic Pattern: ((Null | GreaterP[0]) | Automatic) | Null
    Index Matches to: Sample

    SolventAdditions

    For each extraction round, the solvent(s) that are added to the sample in order to create a biphasic solution.
    Default Value: Automatic
    Default Calculation: Aqueous solvent is automatically added if the starting sample of each extraction round is of Organic or Unknown phase. Organic solvent is automatically added if the starting sample of each extraction round is of Aqueous or Unknown phase. If the sample is already Biphasic, the no solvent is added. Note that the phase of the starting sample in extraction rounds 2 and above is dependent on the TargetPhase and SelectionStrategy options.
    Pattern Description: List of one or more Multiple Solvents or No Solvent or Single Solvent entries.
    Programmatic Pattern: {((ObjectP[{Model[Sample], Object[Sample]}] | _String) | {(ObjectP[{Model[Sample], Object[Sample]}] | _String)..} | None)..} | Automatic
    Index Matches to: Sample

    Demulsifier

    The solution that is added to the sample mixture in order to help promote complete phase separation and avoid emulsions.
    Default Value: Automatic
    Default Calculation: If DemulsifierAdditions is specified, automatically set to the demulsifier specified in DemulsifierAdditions. Otherwise, automatically set to Model[Sample, StockSolution, "5M Sodium Chloride"] if DemulsifierAmount is specified. Otherwise, set to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or None.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | None) | Automatic
    Index Matches to: Sample

    DemulsifierAmount

    The solution that is added to the sample mixture in order to help promote complete phase separation and avoid emulsions.
    Default Value: Automatic
    Default Calculation: Automatically set to 10% of the sample volume if Demulsifier or DemulsifierAdditions is specified. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 milliliters and less than or equal to 200 milliliters or None or Null.
    Programmatic Pattern: ((Null | (RangeP[0*Milliliter, $MaxRoboticTransferVolume] | None)) | Automatic) | Null
    Index Matches to: Sample

    DemulsifierAdditions

    For each extraction round, the Demulsifier that is added to the sample mixture to help promote complete phase separation and avoid emulsions.
    Default Value: Automatic
    Default Calculation: If Demulsifier is not specified, DemulsifierAdditions is set to None. If NumberOfExtractions is set to 1, Demulsifier will only be added during the first extraction round. If NumberOfExtractions is greater than 1 and the sample's Organic phase will be used subsequent extraction rounds (TargetPhase->Aqueous and ExtractionTechnique->Positive OR TargetPhase->Organic and ExtractionTechnique->Negative), Demulsifier will be added during all extraction rounds since the Demulsifier (usually a salt solution which is soluble in the Aqueous layer) will be removed along with the Aqueous layer during the extraction and thus will need to be added before each extraction round. Otherwise, Demulsifier is added to only the first extraction round since the sample's Aqueous phase will be used in subsequent extraction rounds.
    Pattern Description: List of one or more Demulsifier or None entries.
    Programmatic Pattern: {((ObjectP[{Model[Sample], Object[Sample]}] | _String) | None)..} | Automatic
    Index Matches to: Sample

    Temperature

    The set temperature of the incubation device that holds the extraction container during solvent/demulsifier addition, mixing, and settling.
    Default Value: Ambient
    Pattern Description: Ambient or greater than or equal to 0 degrees Celsius and less than or equal to 110 degrees Celsius.
    Programmatic Pattern: RangeP[$MinRoboticIncubationTemperature, $MaxRoboticIncubationTemperature] | Ambient
    Index Matches to: Sample

    NumberOfExtractions

    The number of times that the extraction is performed using the specified extraction parameters using the previous extraction round's impurity layer (after the TargetPhase has been extracted) as the input to subsequent rounds of extraction.
    Default Value: 3
    Pattern Description: Greater than or equal to 1 and less than or equal to 10 in increments of 1.
    Programmatic Pattern: RangeP[1, 10, 1]
    Index Matches to: Sample

    ExtractionMixType

    The style of motion used to mix the sample mixture following the addition of the AqueousSolvent/OrganicSolvent and Demulsifier (if specified).
    Default Value: Automatic
    Default Calculation: Automatically set to Shake if ExtractionMixTime is specified. Otherwise, set to Pipette.
    Pattern Description: Pipette or Shake or Null.
    Programmatic Pattern: ((Null | RoboticMixTypeP) | Automatic) | Null
    Index Matches to: Sample

    ExtractionMixTime

    The duration for which the sample, AqueousSolvent/OrganicSolvent, and Demulsifier (if specified) are mixed.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 Second if ExtractionMixType is set to Shake. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ExtractionMixRate

    The frequency of rotation the mixing instrument uses to mechanically incorporate the sample, AqueousSolvent/OrganicSolvent, and demulsifier (if specified).
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM if ExtractionMixType is set to Shake. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 30 revolutions per minute and less than or equal to 2500 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[$MinRoboticMixRate, $MaxRoboticMixRate]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfExtractionMixes

    The number of times the sample, AqueousSolvent/OrganicSolvent, and demulsifier (if specified) are mixed when ExtractionMixType is set to Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 when ExtractionMixType is set to Pipette. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, $MaxNumberOfMixes, 1]) | Automatic) | Null
    Index Matches to: Sample

    ExtractionMixVolume

    The volume of sample, AqueousSolvent/OrganicSolvent, and demulsifier (if specified) that is mixed when ExtractionMixType is set to Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set to the lesser of 1/2 of the volume of the sample plus any additional components (SampleVolume, AqueousSolventVolume (if specified), OrganicSolventVolume (if specified), and DemulsifierAmount (if specified)) and 970 Microliter (the maximum amount of volume that can be transferred in a single pipetting step on the liquid handling robot) if ExtractionMixType is set to Pipette. Otherwise, set to Null.
    Pattern Description: Greater than or equal to 0 milliliters and less than or equal to 970 microliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Milliliter, $MaxRoboticSingleTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    Settling

    SettlingTime

    The duration for which the sample is allowed to settle and the organic/aqueous phases separate. This is performed after the AqueousSolvent/OrganicSolvent and Demulsifier (if specified) are added and optionally mixed. If ExtractionTechnique is set to PhaseSeparator, the settling time starts once the sample is loaded into the phase separator (the amount of time that we wait for the organic layer to drain through the phase separator's hydrophobic frit).
    Default Value: 1 minute
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: (Null | RangeP[0*Second, $MaxExperimentTime]) | Null
    Index Matches to: Sample

    Centrifuge

    Indicates if the sample is centrifuged to help separate the aqueous and organic layers, after the addition of solvent/demulsifier, mixing, and setting time has elapsed.
    Default Value: Automatic
    Default Calculation: Automatically set to True any of the other centrifuge options are specified (CentrifugeInstrument, CentrifugeIntensity, CentrifugeTime). Also automatically set to True if ExtractionTechnique -> Pipette and the samples are in a centrifuge compatible container (the Footprint of the container is set to Plate). Otherwise, set to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: Sample

    CentrifugeInstrument

    The centrifuge that is used to spin the samples to help separate the aqueous and organic layers, after the addition of solvent/demulsifier, mixing, and setting time has elapsed.
    Default Value: Automatic
    Default Calculation: Automatically set to the integrated centrifuge model that is available in the WorkCell, if the Centrifuge option is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeIntensity

    The rotational speed or the force that is applied to the samples via centrifugation to help separate the aqueous and organic layers, after the addition of solvent/demulsifier, mixing, and setting time has elapsed.
    Default Value: Automatic
    Default Calculation: Automatically set to the lesser of the MaxIntensity of the centrifuge model and the MaxCentrifugationForce of the plate model, if centrifugation is specified.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration])) | Automatic) | Null
    Index Matches to: Sample

    CentrifugeTime

    The amount of time that the samples are centrifuged to help separate the aqueous and organic layers, after the addition of solvent/demulsifier, mixing, and setting time has elapsed.
    Default Value: Automatic
    Default Calculation: Automatically set to 2 Minute, if centrifugation is specified.
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Second, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Collection

    ExtractionBoundaryVolume

    For each extraction round, the volume of the target phase that is either overaspirated via Pipette when IncludeBoundary is set to True (by aspirating the boundary layer along with the TargetPhase and therefore potentially collecting a small amount of the unwanted phase) or underaspirated via Pipette when IncludeBoundary is set to False (by not collecting all of the target phase and therefore reducing the likelihood of collecting any of the unwanted phase). This option only applies if ExtractionTechnique -> Pipette.
    Default Value: Automatic
    Default Calculation: Automatically set the smaller of 10% of the predicted volume of the ExtractionTransferLayer or the volume that corresponds with a 5 Millimeter tall cross-section of the ExtractionContainer at the position of the boundary between aqueous and organic layers if ExtractionTransferLayer -> Top or at the bottom of the container if ExtractionTransferLayer -> Bottom. If ExtractionTechnique -> PhaseSeparator, set to Null.
    Pattern Description: List of one or more greater than or equal to 0 milliliters and less than or equal to 200 milliliters entries or Null.
    Programmatic Pattern: ((Null | {RangeP[0*Milliliter, $MaxRoboticTransferVolume]..}) | Automatic) | Null
    Index Matches to: Sample

    ExtractionTransferLayer

    Indicates whether the top or bottom layer is transferred from the source sample after the organic and aqueous phases are separated. If the TargetLayer matches ExtractionTransferLayer, the sample that is transferred out is the target phase. Otherwise, if TargetLayer doesn't match ExtractionTransferLayer, the sample that remains in the container after the transfer is the target phase.
    Default Value: Automatic
    Default Calculation: Automatically set to Top if ExtractionTechnique->Pipette. Otherwise, set to Null.
    Pattern Description: List of one or more Top or Bottom entries or Null.
    Programmatic Pattern: ((Null | {(Top | Bottom)..}) | Automatic) | Null
    Index Matches to: Sample

    TargetContainerOut

    The container that the separated target layer is transferred into (either via Pipette or PhaseSeparator) after the organic and aqueous phases are separated.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] if TargetPhase -> Organic and ExtractionTechnique -> PhaseSeparator (since the organic layer will flow through the phase separator's hydrophobic frit, TargetContainerOut will be used as the collection container for the phase separator). Otherwise, automatically set to a robotic compatible container that can hold the volume of the target layer via PreferredContainer[...].
    Pattern Description: Existing Container or New Container or New Container with Index.
    Programmatic Pattern: ((ObjectP[Object[Container]] | _String) | ObjectP[Model[Container]] | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}) | Automatic
    Index Matches to: Sample

    TargetContainerOutWell

    The well of the container that the separated target layer is transferred into (either via Pipette or PhaseSeparator) after the organic and aqueous phases are separated.
    Default Value: Automatic
    Default Calculation: Automatically set the first empty well in TargetContainerOut.
    Pattern Description: Any well from A1 to H12.
    Programmatic Pattern: Alternatives @@ Flatten[AllWells[NumberOfWells -> 96]] | Automatic
    Index Matches to: Sample

    ImpurityContainerOut

    The container that the separated impurity layer is transferred into (either via Pipette or PhaseSeparator) after the organic and aqueous phases are separated.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"] if TargetPhase -> Aqueous and ExtractionTechnique -> PhaseSeparator (since the organic impurity layer will flow through the phase separator's hydrophobic frit, ImpurityContainerOut will be used as the collection container for the phase separator). Otherwise, automatically set to a robotic compatible container that can hold the volume of the target layer via PreferredContainer[...].
    Pattern Description: Existing Container or New Container or New Container with Index.
    Programmatic Pattern: ((ObjectP[Object[Container]] | _String) | ObjectP[Model[Container]] | {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}) | Automatic
    Index Matches to: Sample

    ImpurityContainerOutWell

    The well of the container that the separated impurity layer is transferred into (either via Pipette or PhaseSeparator) after the organic and aqueous phases are separated.
    Default Value: Automatic
    Default Calculation: Automatically set the first empty well in ImpurityContainerOut.
    Pattern Description: Any well from A1 to H12.
    Programmatic Pattern: Alternatives @@ Flatten[AllWells[NumberOfWells -> 96]] | Automatic
    Index Matches to: Sample

    TargetStorageCondition

    The condition under which the target sample is stored after the protocol is completed. If left unset, the target sample will be stored under the same condition as the source sample that it originates from.
    Default Value: Null
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Sample

    ImpurityStorageCondition

    The conditions under which the waste layer samples will be stored after the protocol is completed. If left unset, the waste sample will be stored under the same condition as the source sample that it originates from.
    Default Value: Null
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: (Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Null
    Index Matches to: Sample

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Precipitate

    Options

    The options that specify the parameters of this unit operation.
    Pattern: {_Rule..}

    General

    Sample

    The samples which will be precipitated.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Sample] or Object[Container] or a prepared sample or Null.
    Programmatic Pattern: ObjectP[{Object[Sample], Object[Container]}] | _String
    Index Matches to: Sample

    TargetPhase

    Indicates if the target molecules in this sample are expected to be located in the solid precipitate or liquid supernatant after separating the two phases by pelleting or filtration.
    Default Value: Solid
    Pattern Description: Solid or Liquid or Null.
    Programmatic Pattern: (Null | (Solid | Liquid)) | Null
    Index Matches to: Sample

    SeparationTechnique

    Indicates if the the solid precipitate and liquid supernatant are to be separated by centrifugation followed by pipetting of the supernatant (Pellet), or separated by passing the solution through a filter with a pore size large enough to allow the liquid phase to pass through, but not the solid precipitate (Filter).
    Default Value: Pellet
    Pattern Description: Pellet or Filter or Null.
    Programmatic Pattern: (Null | (Pellet | Filter)) | Null
    Index Matches to: Sample

    SampleVolume

    The volume of the SampleIn that will be precipitated.
    Default Value: All
    Pattern Description: All or Volume or Null.
    Programmatic Pattern: (Null | (All | RangeP[0*Microliter, $MaxRoboticTransferVolume])) | Null
    Index Matches to: Sample

    Sterile

    Indicates if the steps of the protocol are performed in a sterile environment.
    Default Value: Automatic
    Default Calculation: Automatically set to False if any samples have CellType set to Null, otherwise Sterile is set to True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    RoboticInstrument

    The instrument that transfers the sample and buffers between containers to execute the protocol.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, LiquidHandler, "bioSTAR"] if Sterile is set to True, and sample contains mammalian cell lines. Or, RoboticInstrument is automatically set to Model[Instrument, LiquidHandler, "microbioSTAR"] if Sterile is set to True and sample contains non-mammalian cell lines. Otherwise RoboticInstrument is set to Model[Instrument, LiquidHandler, "Super STAR"].
    Pattern Description: An object of type or subtype Model[Instrument, LiquidHandler] or Object[Instrument, LiquidHandler]
    Programmatic Pattern: ObjectP[{Model[Instrument, LiquidHandler], Object[Instrument, LiquidHandler]}] | Automatic

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Robotic
    Pattern Description: Robotic.
    Programmatic Pattern: Robotic

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Precipitation

    PrecipitationReagent

    A reagent which, when added to the sample, will help form the precipitate and encourage it to crash out of solution so that it can be collected if it will contain the target molecules, or discarded if the target molecules will only remain in the liquid phase.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, StockSolution, "5M Sodium Chloride"] if TargetPhase is set to Liquid otherwise PrecipitationReagent is set to Model[Sample, "Isopropanol"].
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample.
    Programmatic Pattern: (ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic
    Index Matches to: Sample

    PrecipitationReagentVolume

    The volume of PrecipitationReagent that will be added to the sample to help form the precipitate and encourage it to crash out of solution.
    Default Value: Automatic
    Default Calculation: Automatically set to the lesser of 50% of the input sample's volume or 80% of the maximum volume of the container minus the volume of input samples in order to not overflow the container. PrecipitationReagentVolume will resolve to a minimum of 1 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters.
    Programmatic Pattern: RangeP[0*Microliter, $MaxRoboticTransferVolume] | Automatic
    Index Matches to: Sample

    PrecipitationReagentTemperature

    The temperature that the PrecipitationReagent is incubated at for the PrecipitationReagentEquilibrationTime before being added to the sample, which will help form the precipitate and encourage it to crash out of solution once it has been added to the sample.
    Default Value: Ambient
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: (Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Null
    Index Matches to: Sample

    PrecipitationReagentEquilibrationTime

    The minimum duration for which the PrecipitationReagent will be kept at PrecipitationReagentTemperature before being added to the sample, which will help form the precipitate and encourage it to crash out of solution once it has been added to the sample.
    Default Value: 5 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (Null | RangeP[0*Minute, $MaxExperimentTime]) | Null
    Index Matches to: Sample

    PrecipitationMixType

    The manner in which the sample is agitated following the addition of PrecipitationReagent to the sample, in order to prepare a uniform mixture prior to incubation. Shake indicates that the sample will be placed on a shaker at PrecipitationMixRate for PrecipitationMixTime, while Pipette indicates that PrecipitationMixVolume of the sample will be pipetted up and down for the number of times specified by NumberOfPrecipitationMixes. None indicates that no mixing occurs after adding PrecipitationReagent before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to Pipette if any corresponding Pipette mixing options are set (PrecipitationMixVolume, NumberOfPrecipitationMixes), otherwise PrecipitationMixType is set to Shake.
    Pattern Description: Shake, Pipette, or None or Null.
    Programmatic Pattern: ((Null | (Shake | Pipette | None)) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationMixInstrument

    The instrument used agitate the sample following the addition of PrecipitationReagent, in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"] if PrecipitationMixType is set to Shake and PrecipitationMixTemperature is greater than 70 Celsius. Otherwise PrecipitationMixInstrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"] if PrecipitationMixType is set to Shake.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker] or Object[Instrument, Shaker] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker]}]) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationMixRate

    The number of rotations per minute that the sample and PrecipitationReagent will be shaken at in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM, or the lowest speed PrecipitationMixInstrument is capable of, if PrecipitationMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 3200 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*RPM, $MaxMixRate]) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationMixTemperature

    The temperature at which the mixing device's heating/cooling block is maintained during the PrecipitationMixTime in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if PrecipitationMixType is set to Shake.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationMixTime

    The duration of time that the sample and PrecipitationReagent will be shaken for, at the specified PrecipitationMixRate, in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to 15 Minute if PrecipitationMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfPrecipitationMixes

    The number of times the sample and PrecipitationReagent are mixed by pipetting up and down in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 if PrecipitationMixType is set to Pipette.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, $MaxNumberOfMixes, 1]) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationMixVolume

    The volume of the combined sample and PrecipitationReagent that is displaced during each up and down pipetting cycle in order to prepare a uniform mixture prior to incubation.
    Default Value: Automatic
    Default Calculation: If PrecipitationMixType is set to Pipette, then PrecipitationMixVolume is automatically set to the lesser of 50% of the sample volume plus PrecipitationReagentVolume, or the maximum pipetting volume of Instrument.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 5 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxMicropipetteVolume]) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationTime

    The duration for which the combined sample and PrecipitationReagent are left to settle, at the specified PrecipitationTemperature, in order to encourage crashing of precipitant following any mixing.
    Default Value: 15 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (Null | RangeP[0*Minute, $MaxExperimentTime]) | Null
    Index Matches to: Sample

    PrecipitationInstrument

    The instrument used maintain the sample temperature at PrecipitationTemperature while the sample and PrecipitationReagent are left to settle, in order to encourage crashing of precipitant following any mixing.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"] if PrecipitationTime is greater than 0 Minute and PrecipitationTemperature is greater than 70 Celsius. Otherwise PrecipitationInstrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"] if PrecipitationTime is greater than 0 Minute.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker], Object[Instrument, Shaker], Model[Instrument, HeatBlock], or Object[Instrument, HeatBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker], Model[Instrument, HeatBlock], Object[Instrument, HeatBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    PrecipitationTemperature

    The temperature at which the incubation device's heating/cooling block is maintained during the PrecipitationTime in order to encourage crashing out of precipitant.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if PrecipitationTime is greater than 0 Minute.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    Filtration

    FiltrationInstrument

    The instrument used to apply force to the sample in order to facilitate its passage through the filter. Either by applying centrifugal force to the filter in a centrifuge, or by applying increased air pressure using a pressure manifold.
    Default Value: Automatic
    Default Calculation: If FiltrationTechnique is set to Centrifuge then FiltrationInstrument is set to Model[Instrument, Centrifuge, "VSpin"] if RoboticInstrument is set to STAR, or Model[Instrument, Centrifuge, "HiG4"] if RoboticInstrument is not set to STAR. Otherwise, if FiltrationTechnique is set to AirPressure then FiltrationInstrument is set to Model[Instrument, PressureManifold, "MPE2 Sterile"].
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, PressureManifold], or Object[Instrument, PressureManifold] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, PressureManifold], Object[Instrument, PressureManifold]}]) | Automatic) | Null
    Index Matches to: Sample

    FiltrationTechnique

    The type of dead-end filtration used to apply force to the sample in order to facilitate its passage through the filter. This will be done by either applying centrifugal force to the filter in a centrifuge, or by applying increased air pressure using a pressure manifold.
    Default Value: Automatic
    Default Calculation: Automatically set to AirPressure if SeparationTechnique is set to Filter.
    Pattern Description: Centrifuge or AirPressure or Null.
    Programmatic Pattern: ((Null | (Centrifuge | AirPressure)) | Automatic) | Null
    Index Matches to: Sample

    Filter

    The consumable container with an embedded filter which is used to separate the solid and liquid phases of the sample, after incubation with PrecipitationReagent.
    Default Value: Automatic
    Default Calculation: If SeparationTechnique is set to Filter, then Filter is automatically set to a filter that fits on the filtration instrument (either the centrifuge or pressure manifold) and matches MembraneMaterial and PoreSize if they are set. If the sample is already in a filter, then Filter is automatically set to that filter and the sample will not be transferred to a new filter unless this option is explicitly changed to a new filter.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter] or Object[Container, Plate, Filter] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Model[Container, Plate, Filter], Object[Container, Plate, Filter]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    PrefilterPoreSize

    The pore size of the prefilter membrane, which is placed above Filter, and is designed so that molecules larger than the specified prefilter pore size should not pass through this filter.
    Default Value: Automatic
    Default Calculation: If SeparationTechnique is set to Filter, then PrefilterPoreSize is automatically set to .45 Micron if PrefilterMembraneMaterial is set.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    PrefilterMembraneMaterial

    The material from which the prefilter filtration membrane, which is placed above Filter, is composed. Solvents used should be carefully selected to be compatible with the membrane material.
    Default Value: Automatic
    Default Calculation: Automatically set to GxF if PrefilterPoreSize is specified and SeparationTechnique is set to Filter.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    PoreSize

    The pore size of the filter which is designed such that molecules larger than the specified pore size should not pass through this filter.
    Default Value: Automatic
    Default Calculation: If SeparationTechnique is set to Filter, then PoreSize is automatically set to the PoreSize of Filter, if it is specified. Otherwise, PoreSize is set to .22 Micron if MembraneMaterial is set.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: ((Null | FilterSizeP) | Automatic) | Null
    Index Matches to: Sample

    MembraneMaterial

    The material from which the filtration membrane is composed. Solvents used should be carefully selected to be compatible with the membrane material.
    Default Value: Automatic
    Default Calculation: If SeparationTechnique is set to Filter, then MembraneMaterial is automatically set to the MembraneMaterial of Filter, if it is specified. Otherwise, MembraneMaterial is automatically set to PES if a filter pore size is set.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: ((Null | FilterMembraneMaterialP) | Automatic) | Null
    Index Matches to: Sample

    FilterPosition

    The desired position in the Filter in which the samples are placed for the filtering.
    Default Value: Automatic
    Default Calculation: If the input sample is already in a filter, and SeparationTechnique is set to Filter, then FilterPosition is automatically set to the current position. Otherwise, FilterPosition is set to the first empty position in the filter based on the search order of A1, A2, A3 ... P22, P23, P24.
    Pattern Description: Any well from A1 to P24 or Null.
    Programmatic Pattern: ((Null | WellPositionP) | Automatic) | Null
    Index Matches to: Sample

    FilterCentrifugeIntensity

    The rotational speed or force that will be applied to the sample in order to force the liquid through the filter and facilitate separation of the liquid phase from the insoluble molecules that are larger than the PoreSize of Filter.
    Default Value: Automatic
    Default Calculation: Automatically set to 3600 GravitationalAcceleration if FiltrationInstrument is set to Model[Instrument, Centrifuge, "HiG4"] or 2800 RPM if FiltrationInstrument is set to Model[Instrument, Centrifuge, "VSpin"].
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 80000 revolutions per minute or greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 504000 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, $MaxCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRelativeCentrifugalForce])) | Automatic) | Null
    Index Matches to: Sample

    FiltrationPressure

    The pressure applied to the sample in order to force the liquid through the filter and facilitate separation of the liquid phase from the insoluble molecules that are larger than the PoreSize of Filter.
    Default Value: Automatic
    Default Calculation: Automatically set to 40 PSI if FiltrationTechnique is set to AirPressure.
    Pattern Description: Greater than 0 pounds‐force per inch squared or Null.
    Programmatic Pattern: ((Null | GreaterP[0*PSI]) | Automatic) | Null
    Index Matches to: Sample

    FiltrationTime

    The duration for which the samples will be exposed to either FiltrationPressure or FilterCentrifugeIntensity in order to force the liquid through the filter and facilitate separation of the liquid phase from the insoluble molecules that are larger than the PoreSize of Filter.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if SeparationTechnique is set to Filter.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    FiltrateVolume

    The amount of the filtrate that will be transferred into a new container, after passing through the filter thus having been separated from the molecules too large to pass through the filter.
    Default Value: Automatic
    Default Calculation: Automatically set to 100% of PrecipitationReagentVolume plus the sample volume if SeparationTechnique is set to Filter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    FilterStorageCondition

    When FilterStorageCondition is not set to Disposal, FilterStorageCondition is the set of parameters that define the environmental conditions under which the filter used by this experiment will be stored after the protocol is completed.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Solid, SeparationTechnique is set to Filter, and ResuspensionBuffer is not set to None, then FilterStorageCondition is automatically set to the StorageCondition of the sample. Otherwise, if SeparationTechnique is set to Filter and TargetPhase is set to Liquid, FilterStorageCondition is automatically set to Disposal.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample

    UnprecipitatedSampleContainerOut

    The container that will contain the liquid phase after it is separated from the precipitated solid.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Liquid, then UnprecipitatedSampleContainerOut is automatically set to a container selected by PreferredContainer[...] based on having sufficient capacity to not overflow when the separated liquid phase is added.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}})) | Automatic) | Null
    Index Matches to: Sample

    Pelleting

    PelletVolume

    The expected volume of the pellet after pelleting by centrifugation. This value is used to calculate the distance from the bottom of the container that the pipette tip will be held during aspiration of the supernatant. This calculated distance is such that the pipette tip should be held 2mm above the top of the pellet in order to prevent aspiration of the pellet. Overestimation of PelletVolume will result in less buffer being aspirated while underestimation of PelletVolume will risk aspiration of the pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Microliter if SeparationTechnique is set to Pellet.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 5 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxMicropipetteVolume]) | Automatic) | Null
    Index Matches to: Sample

    PelletCentrifuge

    The centrifuge that will be used to apply centrifugal force to the samples at PelletCentrifugeIntensity for PelletCentrifugeTime in order to facilitate separation by pelleting of insoluble molecules from the liquid phase into a solid phase at the bottom of the container.
    Default Value: Automatic
    Default Calculation: If SeparationTechnique is set to Pellet then PelletCentrifuge is set to Model[Instrument, Centrifuge, "VSpin"] if RoboticInstrument is set to STAR, or Model[Instrument, Centrifuge, "HiG4"] if RoboticInstrument is not set to STAR.
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}]) | Automatic) | Null
    Index Matches to: Sample

    PelletCentrifugeIntensity

    The rotational speed or force that will be applied to the sample to facilitate precipitation of insoluble molecules out of solution.
    Default Value: Automatic
    Default Calculation: Automatically set to 3600 GravitationalAcceleration if PelletCentrifuge is set to Model[Instrument, Centrifuge, "HiG4"] or 2800 RPM if PelletCentrifuge is set to Model[Instrument, Centrifuge, "VSpin"]
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 80000 revolutions per minute or greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 504000 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, $MaxCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRelativeCentrifugalForce])) | Automatic) | Null
    Index Matches to: Sample

    PelletCentrifugeTime

    The duration for which the samples will be centrifuged at PelletCentrifugeIntensity in order to facilitate separation by pelleting of insoluble molecules from the liquid phase into a solid phase at the bottom of the container.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if SeparationTechnique is set to Pellet.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    SupernatantVolume

    The volume of the supernatant that will be transferred to a new container after the insoluble molecules have been pelleted at the bottom of the starting container.
    Default Value: Automatic
    Default Calculation: Automatically set to 90% of the PrecipitationReagentVolume plus the sample volume if SeparationTechnique is set to Pellet.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    Wash

    NumberOfWashes

    The number of times WashSolution is added to the solid, mixed, and then separated again by either pelleting and aspiration if SeparationTechnique is set to Pellet, or by filtration if SeparationTechnique is set to Filter. The wash steps are performed in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to 0 if TargetPhase is set to Liquid, otherwise automatically set to 3.
    Pattern Description: Greater than or equal to 0 and less than or equal to 20 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, 20, 1]) | Automatic) | Null
    Index Matches to: Sample

    WashSolution

    The solution used to help further wash impurities from the solid after the liquid phase has been removed. If SeparationTechnique is set to Filter, then the WashSolution will be added to the filter containing the retentate. If SeparationTechnique is set to Pellet, then the WashSolution will be added to the container containing the pellet. Setting NumberOfWashes to a number greater than 1 will repeat all specified wash steps.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, StockSolution, "70% Ethanol"] if NumberOfWashes is set to a number greater than 0.
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((Null | (ObjectP[{Object[Sample], Model[Sample]}] | _String)) | Automatic) | Null
    Index Matches to: Sample

    WashSolutionVolume

    The volume of WashSolution which will used to help further wash impurities from the solid after the liquid phase has been separated from it. If SeparationTechnique is set to Filter, then this amount of WashSolution will be added to the filter containing the retentate. If SeparationTechnique is set to Pellet, then this amount of WashSolution will be added to the container containing the pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to 100% of the sample starting volume if NumberOfWashes is set to a number greater than 0.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    WashSolutionTemperature

    The temperature at which WashSolution is incubated at during the WashSolutionEquilibrationTime before being added to the solid in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if NumberOfWashes is set to a number greater than 0.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    WashSolutionEquilibrationTime

    The minimum duration for which the WashSolution will be kept at WashSolutionTemperature before being used to help further wash impurities from the solid after the liquid phase has been separated from it.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if NumberOfWashes is set to a number greater than 0.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    WashMixType

    The manner in which the sample is agitated following addition of WashSolution, in order to help further wash impurities from the solid. Shake indicates that the sample will be placed on a shaker at the specified WashMixRate for WashMixTime, while Pipette indicates that WashMixVolume of the sample will be pipetted up and down for the number of times specified by NumberOfWashMixes. None indicates that no mixing occurs before incubation.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Solid and NumberOfWashes is greater than 0, then WashMixType is automatically set to Pipette if any corresponding Pipette mixing options are set (WashMixVolume, NumberOfWashMixes) or if SeparationTechnique is set to Filter. If TargetPhase is set to Solid and NumberOfWashes is greater than 0, then WashMixType is automatically set to Shake if Pipette mixing options are not set and SeparationTechnique is set to Pellet. Otherwise, WashMixType is set to None.
    Pattern Description: Shake, Pipette, or None or Null.
    Programmatic Pattern: ((Null | (Shake | Pipette | None)) | Automatic) | Null
    Index Matches to: Sample

    WashMixInstrument

    The instrument used agitate the sample following the addition of WashSolution in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"] if WashMixType is set to Shake and WashMixTemperature is greater than 70 Celsius. Otherwise WashMixInstrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"] if WashMixType is set to Shake.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker] or Object[Instrument, Shaker] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker]}]) | Automatic) | Null
    Index Matches to: Sample

    WashMixRate

    The rate at which the solid and WashSolution are mixed, for the duration of WashMixTime, in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM, or the lowest speed WashMixInstrument is capable of, if WashMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 3200 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*RPM, $MaxMixRate]) | Automatic) | Null
    Index Matches to: Sample

    WashMixTemperature

    The temperature at which the mixing device's heating/cooling block is maintained for the duration of WashMixTime in order to further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if WashMixType is set to Shake.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    WashMixTime

    The duration for which the solid and WashSolution are mixed at WashMixRate in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if WashMixType is set to Shake .
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfWashMixes

    The number of times WashMixVolume of the WashSolution is mixed by pipetting up and down in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 if WashMixType is set to Pipette.
    Pattern Description: Greater than or equal to 0 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[0, $MaxNumberOfMixes, 1]) | Automatic) | Null
    Index Matches to: Sample

    WashMixVolume

    The volume of WashSolution that is displaced by pipette during each wash mix cycle, for which the number of cycles are defined by NumberOfWashMixes.
    Default Value: Automatic
    Default Calculation: If WashMixType is set to Pipette, then WashMixVolume is automatically set to the lesser of 50% of WashSolutionVolume, or the maximum pipetting volume of Instrument.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 5 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxMicropipetteVolume]) | Automatic) | Null
    Index Matches to: Sample

    WashPrecipitationTime

    The duration for which the samples remain in WashSolution after any mixing has occurred, held at WashPrecipitationTemperature, in order to allow the solid to precipitate back out of solution before separation of WashSolution from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if WashSolutionTemperature is higher than PrecipitationReagentTemperature.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    WashPrecipitationInstrument

    The instrument used to maintain the sample and WashSolution at WashPrecipitationTemperature for the WashPrecipitationTime prior to separation.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"] if WashPrecipitationTime is greater than 0 Minute and WashPrecipitationTemperature is greater than 70 Celsius. Otherwise WashPrecipitationInstrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"] if WashPrecipitationTime is greater than 0 Minute.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker], Object[Instrument, Shaker], Model[Instrument, HeatBlock], or Object[Instrument, HeatBlock] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker], Model[Instrument, HeatBlock], Object[Instrument, HeatBlock]}]) | Automatic) | Null
    Index Matches to: Sample

    WashPrecipitationTemperature

    The temperature which the samples in WashSolution are held at for the duration of WashPrecipitationTime in order to help further wash impurities from the solid.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if WashPrecipitationTime is greater than 0 Minute.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    WashCentrifugeIntensity

    The rotational speed or the force that will be applied to the sample in order to separate the WashSolution from the solid after any mixing and incubation steps have been performed. If SeparationTechnique is set to Filter, then the force is applied to the filter containing the retentate and WashSolution in order to facilitate the solution's passage through the filter and help further wash impurities from the solid. If SeparationTechnique is set to Pellet, then the force is applied to the container containing the pellet and WashSolution in order to encourage the repelleting of the solid.
    Default Value: Automatic
    Default Calculation: If NumberOfWashes is set to a number greater than 0 then WashCentrifugeIntensity is automatically set to 3600 GravitationalAcceleration if either PelletCentrifuge or FiltrationCentrifuge is set to Model[Instrument, Centrifuge, "HiG4"] or 2800 RPM if either PelletCentrifuge or FiltrationCentrifuge is set to Model[Instrument, Centrifuge, "VSpin"].
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 80000 revolutions per minute or greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 504000 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((Null | (RangeP[0*RPM, $MaxCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRelativeCentrifugalForce])) | Automatic) | Null
    Index Matches to: Sample

    WashPressure

    The target pressure applied to the filter containing the retentate and WashSolution in order to facilitate the solution's passage through the filter to help further wash impurities from the retentate.
    Default Value: Automatic
    Default Calculation: If FiltrationTechnique is set to AirPressure and NumberOfWashes is set to a number greater than 0, then WashPressure is automatically set to 40 PSI.
    Pattern Description: Greater than or equal to 0 pounds‐force per inch squared and less than or equal to 40 pounds‐force per inch squared or Null.
    Programmatic Pattern: ((Null | RangeP[0*PSI, $MaxRoboticAirPressure]) | Automatic) | Null
    Index Matches to: Sample

    WashSeparationTime

    The duration for which the samples are exposed to WashPressure or WashCentrifugeIntensity in order to separate the WashSolution from the solid. If SeparationTechnique is set to Filter, then this separation is performed by passing the WashSolution through Filter by applying force of either WashPressure (if FiltrationTechnique is set to AirPressure) or WashCentrifugeIntensity (if FiltrationTechnique is set to Centrifuge). If SeparationTechnique is set to Pellet, then centrifugal force of WashCentrifugeIntensity is applied to encourage the solid to remain as, or return to, a pellet at the bottom of the container.
    Default Value: Automatic
    Default Calculation: If NumberOfWashes greater than 0, then WashSeparationTime is automatically set to 3 Minute if FiltrationTechnique is set to a AirPressure, or otherwise WashSeparationTime is set to 20 Minute.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    DryingTemperature

    The temperature at which the incubation device's heating/cooling block is maintained for the duration of DryingTime after removal of WashSolution, in order to evaporate any residual WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if TargetPhase is set to Solid.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    DryingTime

    The amount of time for which the solid will be exposed to open air at DryingTemperature following final removal of WashSolution, in order to evaporate any residual WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to 20 Minute if TargetPhase is set to Solid.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    Resuspension

    ResuspensionBuffer

    The solution into which the target molecules of the solid will be resuspended or redissolved. Setting ResuspensionBuffer to None indicates that the sample will not be resuspended and that it will be stored as a solid, after any wash steps have been performed.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample, StockSolution, "1x TE Buffer"] if TargetPhase is set to Solid.
    Pattern Description: None or Sample or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | None)) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBufferVolume

    The volume of ResuspensionBuffer that will be added to the solid and mixed in an effort to resuspend or redissolve the solid into the buffer.
    Default Value: Automatic
    Default Calculation: If ResuspensionBuffer is not set to None, then ResuspensionBufferVolume is automatically set to the greater of 25% of the original sample volume, or 10 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxRoboticTransferVolume]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBufferTemperature

    The temperature that the ResuspensionBuffer is incubated at during the ResuspensionBufferEquilibrationTime before being added to the sample in order to resuspend or redissolve the solid into the buffer.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if ResuspensionBuffer is not set to None.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionBufferEquilibrationTime

    The minimum duration for which the ResuspensionBuffer will be kept at ResuspensionBufferTemperature before being added to the sample in order to resuspend or redissolve the solid into the buffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if ResuspensionBuffer is not set to None.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixType

    The manner in which the sample is agitated following addition of ResuspensionBuffer in order to encourage the solid phase to resuspend or redissolve into the buffer. Shake indicates that the sample will be placed on a shaker at the specified ResuspensionMixRate for ResuspensionMixTime at ResuspensionMixTemperature, while Pipette indicates that ResuspensionMixVolume of the sample will be pipetted up and down for the number of times specified by NumberOfResuspensionMixes. None indicates that no mixing occurs after adding ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: If ResuspensionBuffer is not set to None, then ResuspensionMixType is automatically set to Pipette if any corresponding Pipette mixing options are set (ResuspensionMixVolume, NumberOfResuspensionMixes) or if SeparationTechnique is set to Filter. Otherwise ResuspensionMixType is automatically set to Shake.
    Pattern Description: Shake, Pipette, or None or Null.
    Programmatic Pattern: ((Null | (Shake | Pipette | None)) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixInstrument

    The instrument used agitate the sample following the addition of ResuspensionBuffer, in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker, "Inheco Incubator Shaker DWP"] if ResuspensionMixType is set to Shake and ResuspensionMixTemperature is greater than 70 Celsius. Otherwise ResuspensionMixInstrument is set to Model[Instrument, Shaker, "Inheco ThermoshakeAC"] if ResuspensionMixType is set to Shake.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker] or Object[Instrument, Shaker] or Null.
    Programmatic Pattern: ((Null | ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker]}]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixRate

    The rate at which the solid and ResuspensionBuffer are shaken, for the duration of ResuspensionMixTime at ResuspensionMixTemperature, in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 300 RPM, or the lowest speed ResuspensionMixInstrument is capable of, if ResuspensionMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 3200 revolutions per minute or Null.
    Programmatic Pattern: ((Null | RangeP[0*RPM, $MaxMixRate]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTemperature

    The temperature at which the sample and ResuspensionBuffer are held at for the duration of ResuspensionMixTime in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if ResuspensionMixType is set to Shake.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((Null | (Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature])) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixTime

    The duration of time that the solid and ResuspensionBuffer is shaken for, at the specified ResuspensionMixRate, in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 15 Minute if ResuspensionMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: ((Null | RangeP[0*Minute, $MaxExperimentTime]) | Automatic) | Null
    Index Matches to: Sample

    NumberOfResuspensionMixes

    The number of times that the ResuspensionMixVolume of the ResuspensionBuffer and solid are mixed by pipetting up and down in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 if ResuspensionMixType is set to Pipette.
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: ((Null | RangeP[1, $MaxNumberOfMixes, 1]) | Automatic) | Null
    Index Matches to: Sample

    ResuspensionMixVolume

    The volume of ResuspensionBuffer that is displaced during each cycle of mixing by pipetting up and down, which is repeated for the number of times defined by NumberOfResuspensionMixes, in order to encourage the solid to redissolve or resuspend into the ResuspensionBuffer.
    Default Value: Automatic
    Default Calculation: If ResuspensionMixType is set to Pipette, then ResuspensionMixVolume is automatically set to the lesser of 50% of ResuspensionBufferVolume, or the maximum pipetting volume of Instrument.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 5 milliliters or Null.
    Programmatic Pattern: ((Null | RangeP[0*Microliter, $MaxMicropipetteVolume]) | Automatic) | Null
    Index Matches to: Sample

    Sample Storage

    PrecipitatedSampleContainerOut

    The container that contains the resuspended solid isolated after the protocol is completed.
    Default Value: Automatic
    Default Calculation: If ResuspensionBuffer is not set to None, TargetPhase is set to Solid, and SeperationTechinque is set to Filter then ContainerOut is automatically set to a container selected by PreferredContainer[...] based on having sufficient capacity to not overflow when the ResuspensionBufferVolume is added.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or Null.
    Programmatic Pattern: ((Null | ((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> 384]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}})) | Automatic) | Null
    Index Matches to: Sample

    PrecipitatedSampleStorageCondition

    The set of parameters that define the environmental conditions under which the solid that is isolated during precipitation will be stored, either as a solid, or as a solution if ResuspensionBuffer is not set to None.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Solid, then PrecipitatedSampleStorageCondition is automatically set to StorageCondition of SampleIn.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample

    PrecipitatedSampleLabel

    A user defined word or phrase used to identify the solid isolated after precipitation is completed, either as a solid, or as a solution if ResuspensionBuffer is not set to None. If SeparationTechnique is set to Filter, then the sample is the retentate comprised of molecules too large to pass through the filter after precipitation. If SeparationTechnique is set to Pellet, then the sample is the pellet after the supernatant formed during precipitation is removed. This label is for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to "precipitated solid sample #" if TargetPhase is set to Solid.
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    PrecipitatedSampleContainerLabel

    A user defined word or phrase used to identify the container that will contain the solid isolated after precipitation completed, either as a solid, or as a solution if ResuspensionBuffer is not set to None. If SeparationTechnique is set to Filter, then the sample contained in the container is the retentate comprised of molecules too large to pass through the filter after precipitation. If SeparationTechnique is set to Pellet, then the sample contained in the container is the pellet after the supernatant formed during precipitation is removed. This label is for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to "precipitated solid container #" if TargetPhase is set to Solid.
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    UnprecipitatedSampleStorageCondition

    The set of parameters that define the environmental conditions under which the solid that is isolated during precipitation will be stored, either as a solid, or as a solution if ResuspensionBuffer is not set to None.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Liquid, then UnprecipitatedSampleStorageCondition is automatically set to StorageCondition of SampleIn.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: ((Null | ((SampleStorageTypeP | Disposal) | ObjectP[Model[StorageCondition]])) | Automatic) | Null
    Index Matches to: Sample

    UnprecipitatedSampleLabel

    A user defined word or phrase used to identify the liquid phase that is separated during this unit operation. If SeparationTechnique is set to Filter, then the sample is the filtrate after it is separated from the molecules too large to pass through the filter. If SeparationTechnique is set to Pellet, then the sample is the supernatant aspirated after the solid is pelleted using centrifugal force. This label is for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Liquid then LiquidSampleLabel is automatically set to "precipitated liquid phase sample #".
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    UnprecipitatedSampleContainerLabel

    A user defined word or phrase used to identify the container that contains the liquid phase that is separated during this unit operation. If SeparationTechnique is set to Filter, then the sample contained in the container is the filtrate after it is separated from the molecules too large to pass through the filter. If SeparationTechnique is set to Pellet, then the sample contained in the container is the supernatant aspirated after the solid is pelleted using centrifugal force. This label is for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: If TargetPhase is set to Liquid then FiltrateContainerLabel is automatically set to "precipitated liquid phase container #".
    Pattern Description: A string or Null.
    Programmatic Pattern: ((Null | _String) | Automatic) | Null
    Index Matches to: Sample

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Null | (Alternatives[SampleStorageTypeP | Disposal])) | Null
    Index Matches to: Sample

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Experiment Options

    General

    OptimizeUnitOperations

    Indicates if the input list of unit operations should be automatically reordered to optimize the efficiency in which they will be executed in the lab.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    CoverAtEnd

    Indicates if covers are placed on all plates after a group of robotic unit operations to decrease evaporation, if Preparation -> Robotic and WorkCell -> bioSTAR or STAR. Note the qPix does not have the ability to cover plates so CoverAtEnd cannot be set to True for protocols involving PickColonies, StreakCells, or SpreadCells unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to True if OptimizeUnitOperations->True.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic

    Protocol

    Instrument

    Indicates the liquid handler which should be used to perform the provided manipulations.
    Figure 3.1: Unit Operations can be conducted both by a liquid handler or manually with a proper instrument. For some unit operations (e.g., Transfer, Aliquot, and Consolidation), specific options (e.g., DispenseRate and OverAspirationVolume) can only be achieved by a liquid handler.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the containers required by the manipulations.
    Pattern Description: An object of type or subtype Model[Instrument, LiquidHandler] or Object[Instrument, LiquidHandler]
    Programmatic Pattern: ObjectP[{Model[Instrument, LiquidHandler], Object[Instrument, LiquidHandler]}] | Automatic

    TareWeighContainers

    Indicates if empty containers should be tare weighed prior to running the experiment. Tare weighing of each container improves accuracy of any subsequent weighing or gravimetric volume measurement performed in the container.
    Default Value: True
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

Protocol Options

    Organizational Information

    Template

    A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
    Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

    Name

    A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String | Null

    Post Experiment

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to False if the experiment involves Living cells and/or Sterile samples.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to False if the experiment involves Living cells and/or Sterile samples.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to False if the experiment involves Living cells and/or Sterile samples.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Example Calls

    Basic Examples:

    Mix the following sample via pipette:
    Generate a protocol based on a single incubate unit operation. Note that only Plates can be incubated robotically since the robotic incubator can only fit plates:
    Generate a protocol based on a single transfer unit operation:
    Create a new water sample in a 50mL tube and transfer out of it:
    Label a sample in-situ and use it in a later unit operation:

    LabelSample and LabelContainer Examples:

    LabelSample allows you to set certain EHS fields for the sample that is created:
    Multiple samples can be created using a single LabelSample unit operation:
    Transfer a sample into a volumetric flask, and then incubate that sample:
    The Sample option will autofill to the sample used in the last unit operation when not specified:
    If the previous unit operation creates a new sample, that new sample will be used to autofill the next unit operation. In this case, the destination sample created by Transfer will be Incubated:
    Overwriting a container label results in an error message being thrown:

    Transfer Examples:

    When performing the Transfer robotically, the AspirationRate/DispenseRate, OverAspirationVolume/OverDispenseVolume, AspirationWithdrawalRate/DispenseWithdrawalRate, AspirationEquilibrationTime/DispenseEquilibrationTime, AspirationPosition/DispensePosition, AspirationPositionOffset/DispensePositionOffset, CorrectionCurve can be specified. For more Robotic specific options, view the ExperimentTransfer help file:
    New containers can be tagged with a number such that they can be used multiple times within a single transfer protocol:
    If Amount->All is specified, if the Transfer is to be performed Manually, the operator will aspirate/scoop all of the liquid/solid from the source container. If the Transfer is to be performed Robotically, the Robot is told to transfer all of the expected liquid/solid that is in the container (but there is no machine vision to verify that the robot has transferred All of the contents). Be aware that specifying Amount->All means different things depending on the Preparation method:

    Wait Examples:

    Wait for 5 Minutes before starting to perform the next unit operation:

    Incubate/Mix Examples:

    Incubate the sample at 45 Celsius:
    The Mix unit operation is identicaly to the Incubate unit operation, except that it sets the Mix option to True (Incubate sets the Mix option to False by default):
    Mix the sample while also incubating at 80 Celsius:

    Centrifuge Examples:

    Centrifuge mySamples with custom parameters:

    Filter Examples:

    Use the PoreSize option to select a filter that will remove particles larger than the chosen size:
    Use the MolecularWeightCutOff option to select a filter that will remove particles larger than the chosen size:
    After filtering the input samples, wash the retentate with a solvent before resuspending the retentate and transferring to a new container:

    Aliquot Examples:

    To aliquot a specific volume of a sample into a new container:
    To dilute a sample to a new target concentration:
    To dilute a sample to the volume:
    To make multiple, identically sized aliquots of a single source sample, the source needs to be explicitly specified for each intended aliquot. For example, to make five 50 Microliter aliquots from a sample into 0.5 Milliliter tubes:

    Pellet Examples:

    Pellet multiple samples and save all of the supernatant:
    Pellet multiple samples, aspirate all of the supernatant, and resuspend the resulting pellet:
    When being performed robotically, Pellet will attempt to aspirate the supernatant from a PositionOffset of 1.5 Centimeter from the bottom of the container. This is controllabel via the SupernatantTransferAspirationPositionOffset, SupernatantTransferAspirationPosition, SupernatantTransferAspirationRate, and SupernatantTransferAspirationWithdrawalRate options:

    Plate Reader Examples:

    ExperimentRoboticSamplePreparation supports all plate reader experiments that occur on the Model[Instrument, PlateReader, "CLARIOstar"] (AbsorbanceSpectrosocopy, AbsorbanceIntensity, AbsorbanceKinetics, LuminescenceSpectrosocopy, LuminescenceIntensity, LuminescenceKinetics, FluorescenceSpectrosocopy, FluorescenceIntensity, FluorescenceKinetics, and Alphascreen). The CLARIOstar is integrated with the STAR liquid handlers as well as the bioSTAR and microbioSTAR liquid handlers in ExperimentRoboticCellPreparation. If injection samples are used across plate reader unit operations, the injection samples will be consolidated into the same slot in the plate reader unit operation for seamless robotic operation:
    ExperimentRoboticSamplePreparation supports all plate reader experiments that occur on the Model[Instrument, PlateReader, "FLUOstar Omega"] (AbsorbanceSpectrosocopy, AbsorbanceIntensity, AbsorbanceKinetics, LuminescenceIntensity, LuminescenceKinetics, FluorescenceIntensity, and FluorescenceKinetics). The CLARIOstar is integrated with the STAR liquid handlers. If injection samples are used across plate reader unit operations, the injection samples will be consolidated into the same slot in the plate reader unit operation for seamless robotic operation:

Preferred Input Containers

    SBS Format Plates
    2mL and 50mL Tubes

Warnings and Errors

    Messages  (15)

    ContainerLidIncompatible  (1)

    Give an error if a plate is too large to be covered with a lid:

    InvalidUnitOperationHeads  (1)

    If given a unit operation type isn't allowed, throw an error:

    InvalidUnitOperationMethods  (1)

    If given a unit operation method (wrapper head) that isn't supported, throw an error:

    InvalidUnitOperationOptions  (2)

    If given a unit operation with an invalid option, throw an error:

    Throws a message if there are invalid options inside of the given unit operations:

    InvalidUnitOperationRequiredOptions  (2)

    If a unit operation doesn't have its required options filled out, throw an error:

    If given a unit operation with a missing required option, throw an error::

    InvalidUnitOperationValues  (1)

    If given a unit operation with an invalid option value, throw an error:

    MissingObjects  (1)

    If given objects that don't exist, throw an error and return immediately:

    UncoverUnitOperationAdded  (5)

    If a plate is covered in earlier unit operation but required in a later pipet mixing, automatically add an Uncover unit operation:

    If a plate is covered in earlier unit operation but required in a later shaking, there is no need to uncover (1):

    If a plate is covered in earlier unit operation but required in a later shaking, there is no need to uncover (2):

    If a plate is covered in earlier unit operation but required in a later transfer, automatically add an Uncover unit operation:

    No need to add Uncover unit operation if the plate is only covered before the protocol (not inside the protocol) since we have Uncover steps when loading the liquid handler deck:

    WorkCellIsIncompatibleWithMethod  (1)

    WorkCell types bioSTAR and microbioSTAR are only compatible with ExperimentRoboticCellPreparation and STAR is only compatible with ExperimentRoboticSamplePreparation:

Possible Issues

    SBS Microplate Dimensions

    SBS microplates can be used on all robotic liquid handler deck positions designed for such plates; however, when plates are used on certain positions such as HeaterShaker, HeaterCoolerShaker, Tilt, or stacked on top of other plates, the precise positioning of these plates is important. HeaterCooler positions, for example, have a flat table-like protrusion that interfaces with the cavity on the bottom of some plates. For this reason, many dimensions of plates are stored in SLL and accessed by the robotic method compiler to create accurate labware models for use on liquid handling robots. If these dimensions are not correctly measured and recorded, the robotic liquid handler might pipet inaccurately, which could result in inefficient liquid extraction, pipet tips going in the wrong wells, or damage to tips or plates.

    Wide Bore Tip Transfer Dead Volume

    In robotic liquid transfer or pipette mix, when using wide bore Hamilton pipette tips to aspirate samples from VBottom containers, higher dead volumes are required to guarantee the successful transfers. Please make sure the samples have enough volumes if usage of wide bore tips is desired.
Last modified on Thu 19 Dec 2024 17:16:21


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