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ECL`

ExperimentWashCells

ExperimentWashCells[Samples]⟹Protocol

creates a Protocol object to wash living cells in order to remove impurities, debris, metablis, and media from cell samples that prohibits further cell growth or interfers with downstream experiments.

    
Washing Cells is the process of using a solution (usually media or buffer) to remove impurities or unwanted metabolites from a suspension or adherent cell samples. Removing unwanted metabolites is important during cell culture because they can be harmful and potentially kill cells if left accumulating to high levels. For suspension cells, centrifugation is used to separate the cells from the previous media and metabolites. For adherent cultures, the previous media and metabolites are directly aspirated from the cell samples adherent to the surface of the container. After removing the previous media and metabolites, the cells can be washed with a buffer or media which is added to the cells, mixed, and then aspirated off again in order to remove the trace amounts of media and metabolites. The washing procedure can be repeated multiple times; after which cells can be optionally resuspended in media for further growth.
    

Experimental Principles

    Figure 1.1: Procedural overview of a WashCell experiment in which cell isolation technique is Pellet. Step 1: Source media is removed from the cell culture by centrifuging. Step 2: Wash solution is added and mixed with the cells by shaking or pipetting. Step 3: Wash solution is removed by centrifuging. Step 4: Optional media replenishment is applied after Step 2 and Step 3 being repeated for multiple times. The cell sample is optionally mixed with the fresh media by shaking or pipetting.
    Figure 1.2: Procedural overview of a WashCell experiment in which cell isolation technique is Aspirate. Step 1: Source media is removed from the cell culture by directly aspirating from the cell sample adherent to the surface of the container. Step 2: Wash solution is added and mixed with the cells by shaking or pipetting. Step 3: Wash solution is removed by aspirating from the cell sample. Step 4: Optional media replenishement is applied after Step 2 and Step 3 being repeated for multiple times. The cell sample is optionally mixed with the fresh media by shaking or pipetting.

Instrumentation

    bioSTAR

    Figure 2.1.1: A view of the integrations that are attached to the liquid handler instrument deck.
    Figure 2.1.2: Overhead view of the liquid handler instrument deck.

    microbioSTAR

    Figure 2.2: Overhead view of the liquid handler instrument deck.

    Inheco Incubator Shaker DWP

    Figure 2.3: An overview of the Inheco Incubator Shaker DWP. It is capabale of heating and mixing the contents of a single SBS plate by shaking in an enclosed space, which more ideal for warming deep well plates than heaters which heat from the bottom of the plate.

    Inheco Thermoshake

    Figure 2.4: An overview of the Inheco Thermoshake. It is capabale of heating or cooling while mixing the contents of a single SBS plate by shaking.

    HiG4

    Figure 2.5: The HiG4 robotic centrifuge has a swinging bucket rotor that can hold two SBS standard plates. Plates can be loaded and spins can be performed fully robotically. This centrifuge is accessible only using RoboticCellPreparation protocols.

    Hamilton Heater Cooler

    Figure 2.6: An overview of the Hamilton Heater Cooler. It is capabale of heating or cooling a single SBS plate.

Experiment Options

    General

    Method

    The default operating conditions which are used to dissociate cells.
    Default Value: Automatic
    Default Calculation: Automatically set to the object specified in Model[Cell][WashMethod], if any. Automatically set to Custom if no method object is specified for Model[Cell].
    Pattern Description: Custom or Method Object.
    Programmatic Pattern: (Custom | ObjectP[Object[Method, WashCells]]) | Automatic
    Index Matches to: experiment samples

    CellType

    The taxon of the organism or cell line from which the cell sample originates. Options include Bacterial, Mammalian, Insect, Plant, and Yeast.
    Default Value: Automatic
    Default Calculation: Automatically set to the CellType specified in Object[Sample] for the input cell sample.
    Pattern Description: Mammalian, Plant, Insect, Fungal, Yeast, or Bacterial.
    Programmatic Pattern: CellTypeP | Automatic
    Index Matches to: experiment samples

    CultureAdhesion

    Indicates how the cell sample physically interacts with its container. Options include Adherent and Suspension (including any microbial liquid media).
    Default Value: Automatic
    Default Calculation: Automatically set to the CultureAdhesion specified in Object[Sample].
    Pattern Description: Suspension or Adherent.
    Programmatic Pattern: (Suspension | Adherent) | Automatic
    Index Matches to: experiment samples

    CellIsolationTechnique

    The technique used to remove impurities, debris, and media from cell samples. Suspension cells are centrifuged to separate the cells from the media or buffer. Adherent cells remain attached to the bottom of the culture plate whereas the media can be removed via aspiration.
    Default Value: Automatic
    Default Calculation: If CultureAdhesion is Adherent, then set to Aspirate. If CultureAdhesion is set to Suspension and any of the Pellet Options not Null, automatically set to Pellet.
    Pattern Description: Pellet or Aspirate.
    Programmatic Pattern: CellIsolationTechniqueP | Automatic
    Index Matches to: experiment samples

    CellIsolationInstrument

    The instrument used to isolate the cell sample. Centrifuging separates cells from media, forming a cell pellet. The supernatant is removed or harvested, leaving the cell pellet in the container.
    Default Value: Automatic
    Default Calculation: Automatically set to robotic integrated centrifuge, Model[Instrument, Centrifuge, "HiG4"] if CellIsolationTechnique is set to Pellet.
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null
    Index Matches to: experiment samples

    RoboticInstrument

    The instrument that transfers the sample and buffers between containers to execute the protocol.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, LiquidHandler, "bioSTAR"] if CellType is Mammalian. Otherwise, set to Model[Instrument, LiquidHandler, "microbioSTAR"].
    Pattern Description: An object of type or subtype Model[Instrument, LiquidHandler] or Object[Instrument, LiquidHandler]
    Programmatic Pattern: ObjectP[{Model[Instrument, LiquidHandler], Object[Instrument, LiquidHandler]}] | Automatic

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Robotic
    Pattern Description: Robotic.
    Programmatic Pattern: Robotic

    WorkCell

    The automated workstation with a collection of integrated instruments on which this unit operation will be will be performed if Preparation -> Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Media Aspiration

    CellAspirationVolume

    Indicates how much media to remove from an input sample of cells when isolating with Aspriate or Pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to aspirate off the majority of the sample volume, leaving the smaller of 5% of the container's capacity or 100 Microliter.
    Pattern Description: All or greater than or equal to 0 microliters and less than or equal to 200 milliliters.
    Programmatic Pattern: (RangeP[0*Microliter, $MaxRoboticTransferVolume] | All) | Automatic
    Index Matches to: experiment samples

    CellIsolationTime

    The amount of time to centrifuge the cell samples. Centrifuging is intended to separate cells from media.
    Default Value: Automatic
    Default Calculation: Automatically set to 5 min if CellIsolationTechnique is set to Pellet.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    CellPelletContainer

    The container to hold the cell samples in centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set to the deep-well plate whose WorkingVolume is larger than the sample volume if CellIsolationTechnique is set to Pellet.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or Null.
    Programmatic Pattern: (((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}) | Automatic) | Null
    Index Matches to: experiment samples

    CellPelletIntensity

    The rotational speed or force applied to the cell sample by centrifugation. Centrifuging is intended to separate cells from media, forming a cell pellet.
    Default Value: Automatic
    Default Calculation: If CellIsolationTechnique is set to Pellet, automatically set to 300 standard accelerations due to gravity on the surface of the earth if CellType is Mammalian, 2000 standard accelerations due to gravity on the surface of the earth if CellType is Bacterial, 1000 standard accelerations due to gravity on the surface of the earth if CellType is Yeast.
    Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 5703 revolutions per minute or greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 3600 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((RangeP[0*RPM, $MaxRoboticCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRoboticRelativeCentrifugalForce]) | Automatic) | Null
    Index Matches to: experiment samples

    CellAspirationAngle

    Indicates the tilting angle of the adherent cell culture plate when aspirating off the input sample media. See figure 2 of the documentation page.
    Default Value: Automatic
    Default Calculation: Automatically set 10 AngularDegree if CellIsolationTechnique is Aspirate.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees or Null.
    Programmatic Pattern: (RangeP[0*AngularDegree, $MaxRoboticAspirationAngle] | Automatic) | Null
    Index Matches to: experiment samples

    AliquotSourceMedia

    Indicates if sample of source media is collected for future analysis.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any source media options (AliquotMediaVolume, AliquotMediaContainer,...) are not Null.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    AliquotMediaVolume

    The amount of media to collect for analysis after pelleting the cells.
    Default Value: Automatic
    Default Calculation: Automatically set to the same as CellAspirationVolume if AliquotSourceMedia is True.
    Pattern Description: All or greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((RangeP[0*Microliter, $MaxRoboticTransferVolume] | All) | Automatic) | Null
    Index Matches to: experiment samples

    AliquotMediaContainer

    The container used to collect the source media from Media Removal.
    Default Value: Automatic
    Default Calculation: Automatically set to the same container as the input sample if AliquotSourceMedia is True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or Null.
    Programmatic Pattern: (((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}) | Automatic) | Null
    Index Matches to: experiment samples

    AliquotMediaContainerLabel

    A user defined word or phrase used to identify the SourceMediaContainer that contains the source media separated from cell samples, for use in future analysis.
    Default Value: Automatic
    Default Calculation: Automatically set to "source media from cell sample container #" if AliquotSourceMedia is True.
    Pattern Description: A string or Null.
    Programmatic Pattern: (_String | Automatic) | Null
    Index Matches to: experiment samples

    AliquotMediaStorageCondition

    Indicates the conditions under which separated source media are saved after cell isolation.
    Default Value: Automatic
    Default Calculation: Automatically set to freezer if AliquotSourceMedia is True.
    Pattern Description: Storage Object or Storage Type or Null.
    Programmatic Pattern: (((SampleStorageTypeP | Disposal) | ObjectP[{Model[StorageCondition]}]) | Automatic) | Null
    Index Matches to: experiment samples

    AliquotMediaLabel

    A user defined word or phrase used to identify the saved cells sample that is being isolated from media, for use in downstream unit operations.
    Default Value: Automatic
    Default Calculation: Automatically set to "source media from cell sample #" if AliquotSourceMedia is True.
    Pattern Description: A string or Null.
    Programmatic Pattern: (_String | Automatic) | Null
    Index Matches to: experiment samples

    Washing

    NumberOfWashes

    The number of times the sample is washed with WashSolution, prior to replenishment with fresh media, in order to wash trace amounts of media and metabolites from the cells.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 if CultureAdhesion is set to Adherent. Otherwise, set to 2.
    Pattern Description: Greater than or equal to 0 and less than or equal to 20 in increments of 1.
    Programmatic Pattern: RangeP[0, $MaxNumberOfWashes, 1] | Automatic
    Index Matches to: experiment samples

    WashSolution

    The buffer used to wash the cell sample after removing media.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"1x PBS (Phosphate Buffer Saline), Autoclaved"].
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    WashSolutionTemperature

    The temperature of the wash solution.
    Default Value: Automatic
    Default Calculation: If NumberOfWashes is not 0, automatically set to Ambient.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((RangeP[$MinRoboticIncubationTemperature, $MaxRoboticIncubationTemperature] | Ambient) | Automatic) | Null
    Index Matches to: experiment samples

    WashSolutionEquilibrationTime

    The WashSolution will be incubated for a minimum duration of this time at WashSolutionTemperature prior to washing the live cell sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if WashSolutionTemperature is not Null.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    WashVolume

    The amount of WashSolution used.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as CellAspirationVolume.
    Pattern Description: Greater than or equal to 0 milliliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: (RangeP[0*Milliliter, $MaxRoboticTransferVolume] | Automatic) | Null
    Index Matches to: experiment samples

    WashMixType

    Indicates the style of motion (Shake or Pipette) used to mix the cell sample with WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to Shake if any of shake options (WashMixRate, WashMixTime, WashMixInstrument) are set. Otherwise, set to Pipette if any of the pipette options (NumberOfWashMix, WashMixVolume) are set.
    Pattern Description: {Pipette, Shake} or None or Null.
    Programmatic Pattern: ((RoboticMixTypeP | None) | Automatic) | Null
    Index Matches to: experiment samples

    WashMixInstrument

    The instrument used to mix the cell sample with WashSolution via Shake.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker,"Inheco ThermoshakeAC"] if the WashMixType is set to Shake.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker] or Object[Instrument, Shaker] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker]}] | Automatic) | Null
    Index Matches to: experiment samples

    WashMixTime

    Duration of time to mix the cell sample with WashSolution via Shake.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if WashMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    WashMixRate

    The rate at which the sample is mixed with WashSolution via the selected WashMixType for the duration specified by WashMixTime.
    Default Value: Automatic
    Default Calculation: Automatically set to 200 RPM if WashMixType is set to Shake.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 2000 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[$MinBioSTARMixRate, $MaxBioSTARMixRate] | Automatic) | Null
    Index Matches to: experiment samples

    WashMixVolume

    The volume of the cell sample in WashSolution that is pipetted up and down to mix the live cell samples with WashSolution.
    Default Value: Automatic
    Default Calculation: If WashMixType is set to Pipette, automatically set to the smaller of half of the volume of the input sample or 970 Microliter, which is the largest volume that can be pipetted in a single step by the robotic liquid handler.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 970 microliters or Null.
    Programmatic Pattern: (RangeP[0*Microliter, $MaxRoboticSingleTransferVolume] | Automatic) | Null
    Index Matches to: experiment samples

    NumberOfWashMixes

    The number of pipetting cycles (drawing liquid up into the pipette and dispensing back down out of the pipette) used to mix the live cell samples with WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to be the lesser of 50 or 5 * WashVolume/WashMixVolume if WashMixType is set to Pipette.
    Pattern Description: Greater than or equal to 1 and less than or equal to 100 in increments of 1 or Null.
    Programmatic Pattern: (RangeP[1, $MaxNumberOfMixes, 1] | Automatic) | Null
    Index Matches to: experiment samples

    WashTemperature

    The temperature of the device that is used to mix the live cells in WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to WashSolutionTemperature selection if WashMix is True.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((RangeP[$MinRoboticIncubationTemperature, $MaxRoboticIncubationTemperature] | Ambient) | Automatic) | Null
    Index Matches to: experiment samples

    WashAspirationVolume

    Indicates how much wash solution to remove from an input sample of cells prior to media replenishment when isolating with Aspriate or Pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to be the same as WashVolume.
    Pattern Description: All or greater than or equal to 0 microliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: ((RangeP[0*Microliter, $MaxRoboticTransferVolume] | All) | Automatic) | Null
    Index Matches to: experiment samples

    WashIsolationTime

    The amount of time to pellet the cell samples prior to changing media. Centrifuging is intended to separate cells from media.
    Default Value: Automatic
    Default Calculation: Automatically set the same as CellIsolationTime selection if CellIsolationTechnique is set to Pellet.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    WashPelletIntensity

    The rotational speed or force applied to the cell sample by centrifugation prior to changing media. Centrifuging is intended to separate cells from media, forming a cell pellet.
    Default Value: Automatic
    Default Calculation: Automatically set to the same as CellPelletIntensity selection if CellIsolationTechnique is Pellet.
    Pattern Description: Force or Speed or Null.
    Programmatic Pattern: ((RangeP[0*RPM, $MaxRoboticCentrifugeSpeed] | RangeP[0*GravitationalAcceleration, $MaxRoboticRelativeCentrifugalForce]) | Automatic) | Null
    Index Matches to: experiment samples

    WashAspirationAngle

    Indicates the tilting angle of the adherent cell culture plate to aspirate off the wash buffer. The tilt causes the liquid to pool on one edge of the container, thereby making it easier to aspirate off the liquid.
    Default Value: Automatic
    Default Calculation: Automatically set to the CellAspirationAngle selection if CellIsolationTechnique is Aspirate.
    Pattern Description: Greater than or equal to 0 degrees and less than or equal to 10 degrees or Null.
    Programmatic Pattern: (RangeP[0*AngularDegree, $MaxRoboticAspirationAngle] | Automatic) | Null
    Index Matches to: experiment samples

    Media Replenishment

    ResuspensionMedia

    The media used to resuspend the cell pellet after washing with WashSolution.
    Default Value: Automatic
    Default Calculation: Automatically set to the PreferredLiquidMedia of the cell model. Otherwise, set to the same media as the input sample object.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or None.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | None) | Automatic
    Index Matches to: experiment samples

    ResuspensionMediaTemperature

    The temperature of the media used to resuspend the cell sample.
    Default Value: Automatic
    Default Calculation: Automatically set to Ambient if ResuspensionMedia is not None.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((RangeP[$MinRoboticIncubationTemperature, $MaxRoboticIncubationTemperature] | Ambient) | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMediaEquilibrationTime

    Indicates the ResuspensionMedia is incubated at ResuspensionMediaTemperature for at least this amount of time prior to resuspending the cell sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 10 Minute if ResuspensionMedia is not None.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMediaVolume

    The amount of ResuspensionMedia added to the cell sample for media replenishment.
    Default Value: Automatic
    Default Calculation: Automatically set to WorkingVolume of the container object. Otherwise set to 80% of the container's capacity.
    Pattern Description: Greater than or equal to 0 milliliters and less than or equal to 200 milliliters or Null.
    Programmatic Pattern: (RangeP[0*Milliliter, $MaxRoboticTransferVolume] | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMixType

    Indicates the style of motion (Shake or Pipette) used to resuspend the cell sample with ResuspensionMedia. If ResuspensionMixType is set to None, the cells will not be mixed with the ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to Shake if any of ResuspensionMixRate, ResuspensionMixTime, ResuspensionMixInstrument are set. Otherwise, set to Pipette.
    Pattern Description: {Pipette, Shake} or None or Null.
    Programmatic Pattern: ((RoboticMixTypeP | None) | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionTemperature

    The temperature of the device that is used to resuspend the cells in ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to ResuspensionMediaTemperature selection.
    Pattern Description: Ambient or Temperature or Null.
    Programmatic Pattern: ((RangeP[$MinRoboticIncubationTemperature, $MaxRoboticIncubationTemperature] | Ambient) | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMixInstrument

    The instrument used to resuspend the cell sample with ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Instrument, Shaker,"Inheco ThermoshakeAC"] if ResuspensionMixType is Shake.
    Pattern Description: An object of type or subtype Model[Instrument, Shaker] or Object[Instrument, Shaker] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, Shaker], Object[Instrument, Shaker]}] | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMixTime

    Duration of time to mix the cell sample with ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Minute if ResuspensionMixType is set to Shake.
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMixRate

    The shaking rate to mix cell sample with the ResuspensionMedia over the ResuspensionMediaMixTime.
    Default Value: Automatic
    Default Calculation: Automatically set to 200 RPM if ResuspensionMixType is set to Shake. Automatically set to 20 RPM if ResuspensionMixType is set to 20 RPM.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 2000 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[$MinBioSTARMixRate, $MaxBioSTARMixRate] | Automatic) | Null
    Index Matches to: experiment samples

    ResuspensionMixVolume

    The volume of the sample that is pipetted up and down to resuspend the cell sample in ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: If ResuspensionMixType is set to Pipette, automatically set to the smaller of half of the volume of the input sample or 970 Microliter, which is the largest volume that can be pipetted in a single step by the robotic liquid handler.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 970 microliters or Null.
    Programmatic Pattern: (RangeP[0*Microliter, $MaxRoboticSingleTransferVolume] | Automatic) | Null
    Index Matches to: experiment samples

    NumberOfResuspensionMixes

    The number of pipetting cycles (drawing liquid up into the pipette and dispensing back down out of the pipette) used to resuspend the cell sample in ResuspensionMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to be the lesser of 50 or 5 * ResuspensionMediaVolume/ResuspensionMixVolume if ResuspensionMixType is set to Pipette.
    Pattern Description: Greater than or equal to 1 and less than or equal to 100 in increments of 1 or Null.
    Programmatic Pattern: (RangeP[1, $MaxNumberOfMixes, 1] | Automatic) | Null
    Index Matches to: experiment samples

    ReplateCells

    Indicates whether the sample is transferred into new container.
    Default Value: Automatic
    Default Calculation: Automatically set to True if ContainerOut is not Null.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    ContainerOut

    The container into which the resuspended cell sample is transferred into after cell washing and media replenishment.
    Default Value: Automatic
    Default Calculation: Automatically set to the same plate as the input sample if ReplateCells is True.
    Pattern Description: Container or Container with Index or Container with Well or Container with Well and Index or Null.
    Programmatic Pattern: (((ObjectP[{Object[Container], Model[Container]}] | _String) | {GreaterEqualP[1, 1], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], ObjectP[{Object[Container], Model[Container]}]} | {Alternatives @@ Flatten[AllWells[NumberOfWells -> $MaxNumberOfWells]], {GreaterEqualP[1, 1], ObjectP[{Model[Container]}]}}) | Automatic) | Null
    Index Matches to: experiment samples

    ContainerOutLabel

    A user defined word or phrase used to identify the ContainerOut that contains the replated cell samples.
    Default Value: Automatic
    Default Calculation: Automatically set to "wash cell sample container #" if ContainerOut is not Null.
    Pattern Description: A string or Null.
    Programmatic Pattern: (_String | Automatic) | Null
    Index Matches to: experiment samples

    SampleOutLabel

    A user defined word or phrase used to identify the SampleOut that contains the cell samples after WashCells or ChangeMedia.
    Default Value: Automatic
    Default Calculation: Automatically set to "wash cell sample out #".
    Pattern Description: A string.
    Programmatic Pattern: _String | Automatic
    Index Matches to: experiment samples

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null
    Index Matches to: experiment samples

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null
    Index Matches to: experiment samples

Protocol Options

    Organizational Information

    Template

    A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
    Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

    Name

    A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String | Null

    Post Experiment

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: False
    Pattern Description: True or False or Null.
    Programmatic Pattern: (Null | BooleanP) | Null

Example Calls

    Methods

    Perform a Wash Cells experiment on a sample using a present Method:

    CultureAdhesion

    Perform a Wash Cells experiment on suspension cell samples:
    Perform a Wash Cells experiment on adherent cell samples:

    CellIsolationTechnique

    Clarify the desired isolation technique for removing source media and wash solution:

    Pellet Options

    Clarify the options for centrifuging to remove the source media from the cell culture:
    Clarify the options for centrifuging to remove the wash solution from the cell culture:

    Aspirate Options

    Clarify the options for aspirating the source media from the cell sample adherent to the surface of the container:
    Clarify the options for aspirating the wash solution from the cell sample adherent to the surface of the container:

    NumberOfWashes

    Clarify how many times of wash cycles are applied to wash the cell samples:

    Mixing Options

    Clarify the options for mixing the wash solution with the cells during washing:
    Clarify the options for mixing the fresh media with the cells after washing:

Preferred Input Containers

    SBS Format Plates
    2mL and 50mL Tubes

Warnings and Errors

    Messages  (15)

    AspirationVolumeConflictingOption  (1)

    If aspiration volume exceeds the amount of current volume , an error is thrown:

    CellIsolationTechniqueConflictingOptions  (1)

    If CellIsolationTechnique and the given cell isolation options do not match, an error is thrown:

    ExtraneousMediaReplenishmentOptions  (1)

    If ResuspensionMedia is None and any of the resuspension options are specified, an error is thrown:

    ExtraneousWashingOptions  (1)

    If NumberOfWashes is 0 and any of the wash options are specified, an error is thrown:

    ObjectDoesNotExist  (6)

    Throw a message if we have a sample that does not exist (name form):

    Throw a message if we have a container that does not exist (name form):

    Throw a message if we have a sample that does not exist (ID form):

    Throw a message if we have a container that does not exist (ID form):

    Do NOT throw a message if we have a simulated sample but a simulation is specified that indicates that it is simulated:

    Do NOT throw a message if we have a simulated container but a simulation is specified that indicates that it is simulated:

    ReplateCellsConflictingOption  (1)

    If ReplateCells set True for adherent cells, an error is thrown:

    ResuspensionMixTypeConflictingOptions  (1)

    If ResuspensionMixType and the given resuspension mix options do not match, an error is thrown:

    TotalVolumeConflictingOption  (1)

    If addition of volume causes the total volume exceeds the container max volume , an error is thrown:

    WashIsolationTechniqueConflictingOptions  (1)

    If CellIsolationTechnique and the given wash isolation options do not match, an error is thrown:

    WashMixTypeConflictingOptions  (1)

    If WashMixType and the given wash mix options do not match, an error is thrown:

Possible Issues

    Wash Cells change containers

    Washing cells using Pellet as isolation technique must use plates as containers. If the input containers are vessels, cell samples will be transferred into plates before centrifuging.
Last modified on Wed 27 Aug 2025 17:08:24

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