How it Works

  1. How it Works
  2. 1 Design
    Experiment
  3. 2 Execute
    Remotely
  4. 3 Explore
    Data
  5. 4 Analyze
    Results

Run experiments in a central lab from anywhere in the world

1

Design

Ship your samples to Emerald, then design your experiments over the web using our software

2

Execute

Emerald conducts your experiments in an automated lab exactly as you specified

3

Explore

Emerald organizes your data into a database on the cloud which you can access from anywhere

4

Analyze

Ship samples back to your lab and analyze your results using our extensive data analysis suite

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Step 1: Design experiment

Design experiment

Full control over every detail Next

Use the ECL web interface to build your experiment.
Or write commands directly in Emerald's Symbolic Lab Language (SLL).
  • Projects
  • Samples
  • Models
  • Reports
  • Data
  • RNA Origami
  • HIV Studies
    • Line Stores
    • Active Splits
    • 5/1/14 C8166-R5 Split 10 Prep
    • 5/5/14 C8166-R5 Split 11 Prep
      • ID[Sample,Protein,2331]
      • ID[Sample,Protein,2342]
      • ID[Sample,Protein,2345]
      • ID[Sample,Protein,2346]
    • Stains
    • Antibodies
      • ID[Sample,Antibody,549]
      • ID[Sample,Antibody,556]
      • ID[Sample,Antibody,558]
    • Drugs
  • Auto-ligation
  • Neural Progenitors
Commands
  • Plot Data
  • Analyze Data
  • Search Data
  • Share Data
  • Publish Data
  • Compile Experiment
  • Run Simulation
  • Ship Materials
Experiments
  • Analytical Balance Readings
  • Apoptosis Assays
  • Autoclaving
  • Buffer Prep
  • Centrifugation
  • DNA/RNA Synthesis
  • Epifluorescence Microscopy
  • Fast Protein Liquid Chromatography (FPLC)
  • Flash Chromatography
  • Flow Cytometry
  • Fluorescence Kinetics
  • Fluorescence Polarization
  • Fluorescence Spectroscopy
  • Fluorescence Thermodynamics
  • Genomic DNA Prep
  • HPLC (Ion Exchange)
  • HPLC (Reverse Phase)
  • Light Microscopy
  • Liquid Handling
  • Lyophilization
  • MALDI Mass Spectroscopy
  • Mammalian Cell Culture
  • pH Readings
  • Polyacrylamide Gel Electrophoresis (PAGE)
  • Polymerase Chain Reaction (PCR)
  • Protein Extraction
  • Quantitative Real Time PCR (qPCR)
  • RNA Extraction/cDNA Prep
  • Rotary Evaporation
  • Solid Phase Extraction
  • Speedvac Concentration
  • Thermometer Readings
  • Thin Layer Chromatography (TLC)
  • Total Protein Quantification
  • Transfection
  • UV/Vis Kinetics
  • UV/Vis Spectroscopy
  • UV/Vis Thermodynamics
  • Vacuum Filtration
  • Viral Prep
  • Volume Check
  • Western Blot
Protein Samples
Look up sample »
Drag samples here
ID[Sample,Protein,2331] ID[Sample,Protein,2342] ID[Sample,Protein,2346]
Antibody Samples
Look up sample »
Drag samples here
ID[Sample,Antibody,556]
Variables
Replicates 1
Denature False
True
False
Sample Volume 10 (µL)
ul
Antibody Volume 5 (µL)
ul
Luminal Volume 150 (µL)
Peroxide Volume 150 (µL)
HRP Volume 10 (µL)
Primary Washes 2
Primary Wash Volume 150 (µL)
Primary Wash Time 30 (m)
Secondary Washes 1
Secondary Wash Volume 150 (µL)
Secondary Wash Time 30 (m)
Separation Time 25 (m)
m
Separation Voltage 350 (V)
V
Stacking Time 3 (m)
Stacking Voltage 200 (V)
Exposure Time 100 (s)
Number of Blockings 1
Block Volume 200 (µL)
Block Time 5 (m)
Success! Your Western Blot experiment was sent to the Emerald Cloud Lab.
Notebook: HIV Expression.nb
In[1]:= compile[
experiment[WesternBlot],
{ID[Sample,Protein,2331],
ID[Sample,Protein,2342],
ID[Sample,Protein,2346]},
ID[Sample,Antibody,556],
Replicates -> 3,
Denature -> True,
SampleVolume -> 5 Micro Liters,
SeparationTime -> 27 Minutes,
SeparationVoltage -> 375 Volts
]

Out[1]= ID[Experiment,WesternBlot,1314]
ID[Sample,Protein,2331] ID[Sample,Protein,2342] ID[Sample,Protein,2346]
ID[Sample,Antibody,556]

Compile a Western Blot experiment

Select protein and antibody samples from your database

Modify the experiment details as desired

Execute experiment

Results will be added to your database in a matter of days

Step 2: Execute remotely
Execute remotely

Our Robots Do All The Work

The Emerald Cloud Laboratory allows you to execute over 40 different types of standard lab experiments remotely in our central facility, including Flow Cytometry, MALDI, and Western Blot. All of these experiments are conducted using automated instrumentation and robotics to carry out the exact instructions you specified.

Once you submit an experiment to the ECL via our software, the experiment is run automatically and the results are added to your database in just a day or so.

Step 3: Explore data

Explore data

YOUR EXPERIMENTS PRODUCE A WEALTH OF DATA.
EXPLORE THE NETWORK OF ANSWERS.

Data

Database ID
ID[Data,FlowCytometry,7813]
Date
1/10/2012 5:02pm
Side Scatter
{{0.949363 (Seconds),11056 (RFU)},…,{294.889 (Seconds),5195 (RFU)}}
Front Scatter
{{0.949363 (Seconds),2594 (RFU)},…,{294.889 (Seconds),807 (RFU)}}
Fluorescence 530nm
{{0.949363 (Seconds),22 (RFU)},…,{294.889 (Seconds),6214 (RFU)}}
Fluorescence 580nm
{{0.949363 (Seconds),56 (RFU)},…,{294.889 (Seconds),24 (RFU)}}
Fluorescence 676nm
{{0.949363 (Seconds),34 (RFU)},…,{294.889 (Seconds),55 (RFU)}}
Fluorescence 690nm
{{0.949363 (Seconds),94 (RFU)},…,{294.889 (Seconds),84 (RFU)}}

Analysis

Database ID
ID[Analysis,Clustering,4642]
Date
1/11/2012 9:36am
Dimensions
{FrontScatter, Fluorescence580nm}
Method
Kmeans
Distance Function
EuclideanDistance
Number Of Clusters
3
Clustered Data
"{0.949363 (Seconds),…,234.523 (Seconds)}
{1.434466 (Seconds),…,263.423 (Seconds)}
{12.3212 (Seconds),…, 294.889 (Seconds)}"

Experiment

Database ID
ID[Experiment,FlowCytometry,4621]
Date
1/10/2012 4:42pm
Samples In
Flow Rate
100 (µl/min)
Channels
FrontScatter
SideScatter
Fluorescence530nm
Fluorescence580nm
Fluorescence676nm
Fluorescence690nm
Channel Gains
SideScatter -> 560 (V)
Fluorescence530nm -> 780 (V)
Fluorescence580nm -> 800 (V)
Fluorescence676nm -> 780 (V)
Fluorescence690nm -> 820 (V)
Collection Trigger
SideScatter > 3855 (RFU) AND FrontScatter > 3855 (RFU)
Desired Points
5,000

Analysis

Database ID
ID[Analysis,Gating,3216]
Date
1/11/2012 9:34am
Gates
{
  {SideScatter,FrontScatter}->
    Polygon[{{23045,8600},…{24981,5350}}]
}
Gated Data
{0.949363 (Seconds),…,294.889 (Seconds)}

See the experiment that generated this data

Instrument

Database ID
ID[Instrument,FlowCytometer, 21]
Model
Stratedigm SE500
Date Installed
6/24/2011 3:00 pm
Serial Number
SEE5204071811-16
Software
CellCap True v3.1.0.50
Channels
FrontScatter
SideScatter
Fluorescence530nm
Fluorescence580nm
Fluorescence676nm
Fluorescence690nm
Lasers
{488 (nm), 640 (nm)}
Max Flow Rate
150 (µl/min)
Min Flow Rate
20 (µl/min)
Max Channel Gain
1000 (V)
Min Channel Gain
100 (V)
Max Channel Detect
1000 (RFU)
Min Channel Detect
1 (RFU)

Sample

Database ID
ID[Sample,Cells,20943]
Date Created
1/7/2012 8:22 pm
Model Name
CCD-841 CoTr
Cell Type
Adherent
Split Number
16
Storage Conditions
37 (°C), 5 % CO2, >80% Relative Humidity
Media Type
DMEM + 10% FBS
Well
B3
Volume
2 (mL)
Location
Incubator 3, Slot A32

Maintenance

Database ID
ID[Maintenance,Cleaning,52]
Date
1/5/2012 1:45 pm
Status
Successful

Maintenance

Database ID
ID[Maintenance,Cleaning,52]
Date
1/5/2012 1:45 pm
Status
Successful

Maintenance

Database ID
ID[Maintenance,Cleaning,52]
Date
1/5/2012 1:45 pm
Status
Successful

Data

Database ID
ID[Data,Environmental,46850]
Start Date
1/10/2012 3:38pm
End Date
1/10/2012 4:42pm
Temperature
{{3:38:05 (HH:MM:SS), 24.1 (°C)},…,{4:42:27 (HH:MM:SS), 23.7 (°C)}}
Relative Humidity
"{{3:38:05 (HH:MM:SS), 40.1 (%)},…,{4:42:27 (HH:MM:SS), 37.5(%)}}"

Examine the
instruments used

Control

Database ID
ID[Control,Detector,651]
Date
1/12/2012 9:14 am
Control Type
Rainbow Bead Detection
Result
PASSED
Flow Rate
50 (µl/min)
Channels
{FrontScatter, SideScatter, Fluorescence676nm}
Channel Gains
{SideScatter -> 700 (V), Fluorescence676nm -> 950 (V)}
Collection Trigger
SideScatter > 500 (RFU) AND FrontScatter > 500 (RFU)
Desired Points
5,000

See how it all connects

Harness the power
of linked data

The ECL seamlessly stores and allows exploration throughout a connected network of all the information that impacts your data. Relational database objects provide an indexed structure that allows you to easily ask follow-up questions and search through results with just a few keystrokes.

Step 4: Analyze results

Analyze results

A full workstation for data analysis

ECL's Integrated Science Environment is based on the Wolfram Language and provides a wide array of functions to aid in plotting and visualizing scientific data, as well as running physical simulations and calculations.

Experiments, data, analysis, results, and even scientific figures can be exported to files, shared online with team members, or published on the web for all to see.

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  • Projects
  • Samples
  • Models
  • Reports
  • Data
× Notebook: Analyze Examples.nb +

Examples

Check out a small sampling of the more than 5,000 functions available in the ECL.

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