ECL`

AnalyzeDynamicLightScatteringLoading

AnalyzeDynamicLightScatteringLoading[DynamicLightScatteringProtocol]⟹Object

identifies the dynamic light scattering data objects of the protocol with properly loaded samples during experimentation. Thresholding analysis of correlation curve data is used to heuristically group samples that likely failed loading of the instrument.

Details

Loading of the instrument has a known failure rate that is relatively high on the basis of the instrument properties themselves.
Correlation curves at concentrations below 0.1 mg/ml may have correlations starting outside of the range from CorrelationThreshold to CorrelationMaximum. As a result they are not considered improperly loaded.
Data objects that contain many correlation curves, such as those with AssayTypes B22kD, G22, and IsothermalStability, allow for 2 improperly loaded curves before the entire data object is considered improperly loaded.

Input

Output

General Options

Examples

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Basic Examples (3)

Find the data objects that are considered properly loaded:

Plot the correlation curves used in the loading analysis:

Plot the correlation curves used in the loading analysis for cases with multiple samples per data object:

Additional Examples (3)

Find the data from the correlation curves used in the loading analysis for cases with multiple samples per data object:

Find the data that is considered properly loaded without uploading:

Find the excluded data from the correlation curves used in the loading analysis:

Options (7)

CorrelationMaximum (2)

Isolate one correlation curve between the CorrelationThreshold and CorrelationMaximum:

Plot the correlation curves used in the loading analysis with a CorrelationMaximum:

CorrelationTemperature (1)

Plot the final correlation curves used in the loading analysis:

CorrelationThreshold (1)

Plot the correlation curves used in the loading analysis with a lower CorreltionThreshold:

Exclude (1)

Plot the correlation curves used in the loading analysis with an excluded data object:

Include (1)

Plot the correlation curves used in the loading analysis with an included data object:

TimeThreshold (1)

Plot the correlation curves used in the loading analysis with a longer TimeThreshold:

Messages (7)

AbsentIncludeExclude (1)

Include and exclude must already exist in the protocol:

MinExceedsMax (1)

The CorrealtionThreshold cannot exceed the Correlation Minimum:

MissingCorrelationDynamicLightScattering (1)

The data objects are from the Object[Protocol, DynamicLightScattering] do not have the dynamic light scattering corrlation curves for loading analysis:

MissingCorrelationThermalShift (1)

The data objects are from the Object[Protocol, ThermalShift] do not have the dynamic light scattering corrlation curves for loading analysis:

RemovedData (1)

Non-numeric data removed from correlation curves used for thresholding analysis:

SameIncludeExclude (2)

Include and exclude cannot contain any of the same members:

Last modified on Mon 31 Oct 2022 10:58:03