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ECL`

PlotqPCR

PlotqPCR[QPCRData]Fig

plots the normalized and baseline-subtracted amplification curves from QPCRData.

PlotqPCR[Protocol]Plot

creates a Plot of the amplification curves in the data objects found in the Data field of Protocol.

PlotqPCR[MeltingPointAnalysis]Fig

plots the negative derivative of the melting curve data MeltingPointAnalysis.

PlotqPCR[AmplificationCurveData]Fig

plots raw AmplificationCurveData.

Details

  • For each input quantitative polymerase chain reaction (qPCR) data object, if there are linked analysis objects, the quantification cycle/copy number information from the most recent analysis for each applicable wavelength pair is displayed as a tooltip.
  • Input
    Output
    Data Specifications Options
    Fractions Options
    Frame Options
    Grid Options
    Image Format Options
    Ladder Options
    Legend Options
    Peaks Options
    Plot Labeling Options
    Plot Range Options
    Plot Style Options
    Secondary Data Options
    General Options

Examples

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Basic Examples  (4)

Given an Object[Analysis, MeltingPoint] packet generated from Object[Data, qPCR], plot the melting curve as the negative derivative:

Given a qPCR data object, creates a plot for the applicable normalized and baseline-subtracted amplification curves. If there are linked analysis objects, the quantification cycle/copy number information from the most recent analysis for each applicable wavelength pair is displayed as a tooltip:

Given a qPCR protocol object, creates a plot for the linked data objects. If there are linked analysis objects, the quantification cycle/copy number information from the most recent analysis for each applicable wavelength pair is displayed as a tooltip:

Given multiple qPCR data objects, creates a plot for the applicable normalized and baseline-subtracted amplification curves. For each data object, if there are linked analysis objects, the quantification cycle/copy number information from the most recent analysis for each applicable wavelength pair is displayed as a tooltip:

Options  (7)

BaselineDomain  (3)

BaselineDomain can be specified:

BaselineDomain is automatically set to {3 Cycle, 15 Cycle} if there are no linked quantification cycle analysis object:

BaselineDomain is automatically set to the BaselineDomain from the most recent quantification cycle analysis object:

Basic  (2)

Set option PrimaryData->MeltingCurves to plot the melting curve:

Set option PrimaryData->MeltingCurves to plot the melting curve. If a melting point analysis has been performed on the object, the analysis data will be used directly:

CurveType  (2)

CurveType can be specified as multiple types:

CurveType can be specified as one type:

Messages  (1)

NoqPCRMeltingCurveData  (1)

If no melting curve data is available, an error is thrown:

Last modified on Wed 27 Aug 2025 17:40:55