Object[Method, FlowCytometry]
A method containing optics settings to run a flow cytometer experiment.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Method, FlowCytometry, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Method, FlowCytometry]
Pattern Description: Object[Method, FlowCytometry]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
General
Template
The method whose option selections should be selected as the default options for this method.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method][MethodsTemplated]
MethodsTemplated
Methods which were generated using this method's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method][Template]
Detector
The detectors which should be used to detect light scattered off the samples.
Format: Multiple
Class: Expression
Programmatic Pattern: FlowCytometryDetectorP
DetectionLabel
For each member of Detector, the tag, including fluorescent or non-fluorescent chemical compounds or proteins, attached to the sample that will be analyzed by the Detectors.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Alternatives[Model[Molecule]]
ExcitationWavelength
The wavelength(s) which should be used to excite fluorescence and scatter off the samples.
Format: Multiple
Class: Expression
Programmatic Pattern: FlowCytometryExcitationWavelengthP
ExcitationPower
For each member of ExcitationWavelength, the power which should be used to excite fluorescence and scatter off the samples.
Format: Multiple
Class: Real
Unit: 1 milliwatt
Programmatic Pattern: RangeP[0 Milli Watt, 100 Milli Watt]
Gain
For each member of Detector, the voltage the PMT should be set to to detect the scattered light off the sample.
Format: Multiple
Class: Real
Unit: 1 volt
Programmatic Pattern: GreaterEqualP[0 Volt]
NeutralDensityFilter
For each member of Detector, if a neutral density filter with an optical density of 2.0 should be used lower the intensity of scattered light that hits the detector.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
Last modified on Mon 26 Sep 2022 15:43:47