Object[Method, Gradient]
A method containing parameters specifying a solvent gradient utilized by an HPLC run.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Method, Gradient, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Method, Gradient]
Pattern Description: Object[Method, Gradient]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
General
Template
The method whose option selections should be selected as the default options for this method.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method][MethodsTemplated]
MethodsTemplated
Methods which were generated using this method's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method][Template]
Temperature
The temperature at which the gradient is run.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
FlowRate
The speed of the mobile phase over time.
Format: Single
Class: Expression
Programmatic Pattern: 0 (Liter Milli) 0 (Liter Milli)
Hold[{{GreaterEqualP[0 Minute], GreaterEqualP[---------------]}...} | GreaterEqualP[---------------]]
Minute Minute
InitialFlowRate
The starting flow rate used by this gradient.
Format: Single
Class: Real
Unit: 1 milliliter per minute
Programmatic Pattern: 0 (Liter Milli)
Hold[GreaterEqualP[---------------]]
Minute
GradientA
The percentage of Buffer A in the composition over time, in the form: {Time, % Buffer A}.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientB
The percentage of Buffer B in the composition over time, in the form: {Time, % Buffer B}.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientC
The percentage of Buffer C in the composition over time, in the form: {Time, % Buffer C}.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientD
The percentage of Buffer D in the composition over time, in the form: {Time, % Buffer D}.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientE
The percentage of BufferE in the composition over time, in the form: {Time, % BufferE} or a single % BufferE for the entire run.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientF
The percentage of BufferF in the composition over time, in the form: {Time, % BufferF} or a single % BufferF for the entire run.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientG
The percentage of BufferG in the composition over time, in the form: {Time, % BufferG} or a single % BufferG for the entire run.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientH
The percentage of BufferH in the composition over time, in the form: {Time, % BufferH} or a single % BufferH for the entire run.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
Gradient
Definition of buffer composition as a function of time where composition at the points in between is assumed to be a line joining the points.
Format: Multiple
[[1]] Time
Header: Time
Class: Real
Unit: 1 minute
[[2]] Buffer A Composition
Header: Buffer A Composition
Class: Real
Unit: 1 percent
[[3]] Buffer B Composition
Header: Buffer B Composition
Class: Real
Unit: 1 percent
[[4]] Buffer C Composition
Header: Buffer C Composition
Class: Real
Unit: 1 percent
[[5]] Buffer D Composition
Header: Buffer D Composition
Class: Real
Unit: 1 percent
[[6]] Buffer E Composition
Header: Buffer E Composition
Class: Real
Unit: 1 percent
[[7]] Buffer F Composition
Header: Buffer F Composition
Class: Real
Unit: 1 percent
[[8]] Buffer G Composition
Header: Buffer G Composition
Class: Real
Unit: 1 percent
[[9]] Buffer H Composition
Header: Buffer H Composition
Class: Real
Unit: 1 percent
[[10]] Flow Rate
Header: Flow Rate
Class: Real
Unit: 1 milliliter per minute
RefractiveIndexReference Loading
For each member of Gradient, the status of HPLC refactive index detector reference loading valves. When set to Open, buffer and sample are loaded into the reference cell during this gradient step.
Format: Multiple
Class: Expression
Programmatic Pattern: Open | Closed
GradientStart
For linear gradients, the initial Buffer B percentage.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: PercentP
GradientEnd
For linear gradients, the final Buffer B percentage.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: PercentP
GradientDuration
For linear gradients, the duration to reach GradientEnd from GradientStart.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: TimeP
EquilibrationTime
The amount of time to equilibrate at GradientStart before starting the gradient.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: TimeP
FlushTime
The amount of time to flush at 100% Buffer B at the end of the gradient.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: TimeP
Reagents
BufferA
The first solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferB
The second solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferC
The third solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferD
The four solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferE
The fifth solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferF
The sixth solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferG
The seventh solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
BufferH
The eighth solution used in the mobile phase.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Sample]
Last modified on Mon 26 Sep 2022 15:43:47