Name of this Object.

ID of this Object.

Object of this Object.

Type of this Object.

Notebook this object belongs to.

The method whose option selections should be selected as the default options for this method.

Methods which were generated using this method's options as a starting point for its option defaults.

The type of cell culture (adherent or suspension) that should be used to grow these cells. Used to set the WashMethod and ContainerOut.

Indicates if the cell samples should be washed with a PredissociationWashSolution before Dissociation occurs. This option cannot be set if dealing with non-adherent cells.

The solution that is used to wash the cell samples, before Dissociation occurs.

The amount of PredissociationWash solution that should be applied to the cell samples.

The pipette that will be used to transfer the PredissociationWashSolution into the ContainerIn. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the PredissociationWashSolution into the ContainerIn.

The temperature to heat/cool the PredissociationWashSolution to before the PredissociationWash occurs.

The duration of time for which the PredissociationWashSolution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target PredissociationWashSolutionTemperature, if they do not reach the PredissociationWashSolutionTemperature after PredissociationWashSolutionEquilibrationTime.

The method by which to verify the temperature of the PredissociationWash solution before the transfer is performed.

Indicates if mixing should occur during aspiration from the PredissociationWash solution.

The type of mixing that should occur during aspiration from the PredissociationWash solution.

The number of times that the PredissociationWash solution should be mixed during aspiration.

Indicates if mixing should occur after the PredissociationWashSolution is dispensed into the ContainerIn.

The type of mixing that should occur after the PredissociationWashSolution is dispensed into the ContainerIn.

The number of times that the PredissociationWashSolution should be mixed after the PredissociationWashSolution is dispensed into the ContainerIn.

The number of times that the cell samples should be washed before dissociation occurs.

Indicates whether the cells should be washed, before they're combined with fresh media.

The number of times that the cells should be washed with WashSolution.

The method by which the cells should be washed.

The solution that is used to wash the cells.

The pipette that will be used to transfer the WashSolution into the WashContainer. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the WashSolution into the WashContainer.

The container that the cells will be placed in when they are washed.

The amount of wash solution that is added to the WashContainer when the cells are washed.

The temperature to heat/cool the wash solution to before the washing occurs.

The duration of time for which the wash solution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target WashSolutionTemperature, if they do not reach the WashSolutionTemperature after WashSolutionEquilibrationTime.

The method by which to verify the temperature of the wash solution before the transfer is performed.

The temperature to heat/cool the wash container to before the washing occurs.

The duration of time for which the wash container will be heated/cooled to the target temperature.

The maximum duration of time for which the wash container will be heated/cooled to the target WashContainerTemperature, if they do not reach the WashContainerTemperature after WashContainerEquilibrationTime.

The method by which to verify the temperature of the wash container before the transfer is performed.

Indicates if mixing should occur during aspiration from the wash solution.

The type of mixing that should occur during aspiration from the wash solution.

The number of times that the wash solution should be mixed during aspiration.

Indicates if mixing should occur after the cells are dispensed into the wash solution.

The type of mixing that should occur after the cells are dispensed into the wash solution.

The number of times that the cell/wash solution suspension should be mixed after dispension.

Indicates if the cells should be incubated and/or mixed after the wash solution is added.

The instrument used to perform the Mixing and/or Incubation of the WashSolution.

The amount of time that the cells should be incubated/mixed with the wash solution.

The temperature of the mix instrument while mixing/incubating the cells with the wash solution.

Indicates if this cells and wash solution should be mixed during WashSolutionIncubation.

Indicates the style of motion used to mix the sample.

Frequency of rotation of the mixing instrument should use to mix the cells/wash solution.

The centrifuge that will be used to pellet the provided samples.

The rotational speed or the force that will be applied to the samples by centrifugation in order to create a pellet.

The amount of time that the samples will be centrifuged.

The temperature at which the centrifuge chamber will be held while the samples are being centrifuged.

Indicates if the cell samples are adherent and first need to be dissociated from the flask/plate surface before they can be split.

The solution that is used to dissociate the adherent cells from their ContainerIn(s).

The amount of Dissociation solution that should be applied to the cell samples.

The pipette that will be used to transfer the DissociationSolution into the ContainerIn. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the DissociationSolution into the ContainerIn.

The temperature to heat/cool the DissociationSolution to before the Dissociation occurs.

The duration of time for which the DissociationSolution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target DissociationSolutionTemperature, if they do not reach the DissociationSolutionTemperature after DissociationSolutionEquilibrationTime.

The method by which to verify the temperature of the Dissociation solution before the transfer is performed.

Indicates if mixing should occur during aspiration from the Dissociation solution.

The type of mixing that should occur during aspiration from the Dissociation solution.

The number of times that the Dissociation solution should be mixed during aspiration.

Indicates if mixing should occur after the DissociationSolution is dispensed into the ContainerIn.

The type of mixing that should occur after the DissociationSolution is dispensed into the ContainerIn.

The number of times that the DissociationSolution should be mixed after the DissociationSolution is dispensed into the ContainerIn.

Indicates if the dissociation should be continued up to the MaxDissociationTime (after DissociationTime has passed), in an attempt dissociate the cells from the ContainerIn surface.

The amount of time that the cells should be incubated with the Dissociation solution.

The maximum amount of time that the cells should be incubated with the Dissociation solution.

Indicates if the sample should have a DissociationInactivationSolution added after the Dissociation step to inactive the DissociationSolution.

The solution that is used to inactivate the DissociationSolution after DissociationTime/MaxDissociationTime has passed.

The amount of DissociationInactivation solution that should be applied to the cell samples.

The pipette that will be used to transfer the DissociationInactivationSolution into the ContainerIn. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the DissociationInactivationSolution into the ContainerIn.

The temperature to heat/cool the DissociationInactivationSolution to before the DissociationInactivation occurs.

The duration of time for which the DissociationInactivationSolution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target DissociationInactivationSolutionTemperature, if they do not reach the DissociationInactivationSolutionTemperature after DissociationInactivationSolutionEquilibrationTime.

The method by which to verify the temperature of the DissociationInactivation solution before the transfer is performed.

Indicates if mixing should occur during aspiration from the DissociationInactivation solution.

The type of mixing that should occur during aspiration from the DissociationInactivation solution.

The number of times that the DissociationInactivation solution should be mixed during aspiration.

Indicates if mixing should occur after the DissociationInactivationSolution is dispensed into the ContainerIn.

The type of mixing that should occur after the DissociationInactivationSolution is dispensed into the ContainerIn.

The number of times that the DissociationInactivationSolution should be mixed after the DissociationInactivationSolution is dispensed into the ContainerIn.

Indicates if a cell scraper should be used in order to help the dissociation of the adherent cells from the bottom of the cell flask.

The scraper that should be used to physically help dissociate the adherent cells from the bottom of the cell flask.

Indicates if scraping should occur before or after the DissociationSolution is added, if both Dissociation and ScrapeCells are specified.

Indicates if the ContainerOut should first be coated with a CoatingSolution before the cells are plated.

The solution that is used to coat the ContainerOut.

The pipette that will be used to transfer the CoatingSolution into the ContainerOut. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the CoatingSolution into the ContainerOut.

The amount of coating solution that should be applied to the ContainerOut.

The temperature to heat/cool the coating solution to before the coating occurs.

The duration of time for which the coating solution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target CoatingSolutionTemperature, if they do not reach the CoatingSolutionTemperature after CoatingSolutionEquilibrationTime.

The method by which to verify the temperature of the coating solution before the transfer is performed.

The temperature to heat/cool the wash container to before the coating occurs.

The duration of time for which the wash container will be heated/cooled to the target temperature.

The maximum duration of time for which the wash container will be heated/cooled to the target WashContainerTemperature, if they do not reach the WashContainerTemperature after WashContainerEquilibrationTime.

The method by which to verify the temperature of the wash container before the transfer is performed.

Indicates if mixing should occur during aspiration from the coating solution.

The type of mixing that should occur during aspiration from the coating solution.

The number of times that the coating solution should be mixed during aspiration.

Indicates if mixing should occur after the cells are dispensed into the coating solution.

The type of mixing that should occur after the cells are dispensed into the coating solution.

The number of times that the cell/coating solution suspension should be mixed after dispension.

Indicates if the cells should be incubated and/or mixed after the coating solution is added.

The instrument used to perform the Mixing and/or Incubation of the CoatingSolution.

The amount of time that the cells should be incubated/mixed with the coating solution.

The temperature of the mix instrument while mixing/incubating the cells with the coating solution.

Indicates if this cells and coating solution should be mixed during CoatingSolutionIncubation.

Indicates the style of motion used to mix the sample.

Frequency of rotation of the mixing instrument should use to mix the cells/coating solution.

Indicates if the ContainerOut should first be coated with a CoatingWashSolution before the cells are plated.

The solution that is used to coat the ContainerOut.

The pipette that will be used to transfer the CoatingWashSolution into the ContainerOut. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the CoatingWashSolution into the ContainerOut.

The amount of CoatingWash solution that should be applied to the ContainerOut.

The temperature to heat/cool the CoatingWash solution to before the CoatingWash occurs.

The duration of time for which the CoatingWash solution will be heated/cooled to the target temperature.

The maximum duration of time for which the samples will be heated/cooled to the target CoatingWashSolutionTemperature, if they do not reach the CoatingWashSolutionTemperature after CoatingWashSolutionEquilibrationTime.

The method by which to verify the temperature of the CoatingWash solution before the transfer is performed.

The temperature to heat/cool the wash container to before the CoatingWash occurs.

The duration of time for which the wash container will be heated/cooled to the target temperature.

The maximum duration of time for which the wash container will be heated/cooled to the target WashContainerTemperature, if they do not reach the WashContainerTemperature after WashContainerEquilibrationTime.

The method by which to verify the temperature of the wash container before the transfer is performed.

Indicates if mixing should occur during aspiration from the CoatingWash solution.

The type of mixing that should occur during aspiration from the CoatingWash solution.

The number of times that the CoatingWash solution should be mixed during aspiration.

Indicates if mixing should occur after the cells are dispensed into the CoatingWash solution.

The type of mixing that should occur after the cells are dispensed into the CoatingWash solution.

The number of times that the cell/CoatingWash solution suspension should be mixed after dispension.

Indicates if the cells should be incubated and/or mixed after the CoatingWash solution is added.

The instrument used to perform the Mixing and/or Incubation of the CoatingWashSolution.

The amount of time that the cells should be incubated/mixed with the CoatingWash solution.

The temperature of the mix instrument while mixing/incubating the cells with the CoatingWash solution.

Indicates if this cells and CoatingWash solution should be mixed during CoatingWashSolutionIncubation.

Indicates the style of motion used to mix the sample.

Frequency of rotation of the mixing instrument should use to mix the cells/CoatingWash solution.

Indicates if fresh media should be added once the wash solution is aspirated from the cells.

The media that is used to grow the washed cells.

The container that will be used to plate the washed cells and new media.

The pipette that will be used to transfer the the media/washed cells into the ContainerOut. If this pipette is the same model as the Pipette option, the same pipette will be used.

The tips used to transfer the Media into the ContainerOut.

The amount of Media to add to the ContainerOut.

The temperature to heat/cool the media to before the washing occurs.

The duration of time for which the media will be heated/cooled to the target temperature.

The maximum duration of time for which the media will be heated/cooled to the target MediaTemperature, if they do not reach the MediaTemperature after MediaEquilibrationTime.

The method by which to verify the temperature of the media before the transfer is performed.

The temperature to heat/cool the ContainerOut to before the washing occurs.

The duration of time for which the ContainerOut will be heated/cooled to the target temperature.

The maximum duration of time for which the ContainerOut will be heated/cooled to the target ContainerOutTemperature, if they do not reach the ContainerOutTemperature after ContainerOutEquilibrationTime.

The method by which to verify the temperature of the ContainerOut before the transfer is performed.

Indicates if mixing should occur during aspiration from the original media container.

The type of mixing that should occur during aspiration from the original media container.

The number of times that the media should be mixed during aspiration.

Indicates if mixing should occur after the media is dispensed into the washed cells.

The type of mixing that should occur after the media is dispensed into the washed cells.

The number of times that the cell/media suspension should be mixed after dispension.

The ratio of the cells in the cell sample that should be plated in the ContainerOut.

The volume of cell suspension, from the SampleIn, that will be plated in the ContainerOut, for each replicate. This option only applies to suspension cells that are not being washed.