Object[Protocol, CapillaryGelElectrophoresisSDS]
A protocol for performing a Capillary gel Electrophoresis-Sodium Dodecyl Sulfate (CESDS) experiment on samples to separate denatured proteins according to their molecular weight by electrophoresis through a sieving matrix.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryGelElectrophoresisSDS, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryGelElectrophoresisSDS]
Pattern Description: Object[Protocol, CapillaryGelElectrophoresisSDS]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The device on which the protocol is run. The instrument is loaded with a cartridge that holds the capillary in which Capillary gel Electrophoresis-Sodium Dodecyl Sulfate (CESDS) is performed as well as the anode running buffer required for this experiment. Samples are first denatured by heating in the presence of SDS, imparting all proteins in the sample a negative charge and a similar charge to mass ratio. For every sample, separation matrix is loaded onto the capillary and the sample is electroinjected into the matrix. Subsequently, a voltage is applied on the capillary and proteins migrate through the sieving matrix where smaller proteins migrate faster than larger ones. Proteins are detected as they migrate past a UV absorbance detector and relative migration times are calculated relative to an internal standard.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayPlate
The 96-well plate holding all reagents and samples for a Capillary gel Electrophoresis-SDS experiment loaded onto the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
SamplePreparationPlate
The 96-well plate in which sample preparation for a Capillary gel Electrophoresis-SDS experiment takes place.
Format: Single
Class: Link
Programmatic Pattern: _Link
RunTime
The total time capillary gel electrophoresis sds is expected to run.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Second]
InjectionTable
The sequence of injections for a given experiment run for SamplesIn, Ladders, Standards, and Blanks.
Format: Multiple
[Type]
Header: Type
Class: Expression
[Sample]
Header: Sample
Class: Link
[Volume]
Header: Volume
Class: Real
Unit: 1 microliter
[Data]
Header: Data
Class: Link
[SampleIndex]
Header: SampleIndex
Class: Integer
MethodFile
The methods file containing the run parameters for all samples in protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InjectionListFile
The file specifying the order of sample injection and associated method names for all samples in the protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
Denature
Indicates if heat denaturation should be applied to all samples.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
DenaturingTemperature
Indicates the temperature to which samples will be heated in order to linearize proteins in the sample.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
DenaturingTime
Indicates the duration samples will be incubated at the DenaturingTemperature.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Second]
CoolingTemperature
Indicates the temperature at which samples will be cooled after denaturation.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
CoolingTime
Indicates the duration samples will be incubated at the CoolingTemperatures.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Second]
PelletSedimentation
Indicates if centrifugation should be applied to the sample to remove precipitates after denaturation.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
SedimentationCentrifugation Speed
Indicates the speed of centrifugation applied to remove precipitates.
Format: Single
Class: Integer
Unit: 1 revolution per minute
Programmatic Pattern: GreaterP[0 RPM]
SedimentationCentrifugation Force
Indicates the force of centrifugation applied to remove precipitates.
Format: Single
Class: Integer
Unit: 1 standard acceleration due to gravity on the surface of the earth
Programmatic Pattern: GreaterP[0 GravitationalAcceleration]
SedimentationCentrifugation Time
Indicates the duration samples will be centrifuged to remove precipitates.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Second]
SedimentationCentrifugation Temperature
Indicates the temperature at which samples will be held while centrifugating to remove precipitates.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
AssayContainers
The containers the samples are assayed in.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
TotalVolumes
For each member of SamplesIn, indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains Sample, InternalReference, SDSBuffer, and ReducingAgent and/or AlkylatingAgent.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SampleVolumes
For each member of SamplesIn, indicates the volume of sample required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PremadeMasterMixReagents
For each member of SamplesIn, indicates the premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PremadeMasterMixDiluents
For each member of SamplesIn,indicates the solution used to dilute the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PremadeMasterMixReagent DilutionFactors
For each member of SamplesIn, indicates the factor by which the premade mastermix should be diluted by in the final assay tube.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[1]
PremadeMasterMixVolumes
For each member of SamplesIn, indicates the volume of the premade mastermix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
InternalReferences
For each member of SamplesIn, indicates the internal standard stock solution added in sample preparation. The internal standard stock solution contains the analyte that serves as the reference by which Relative Migration Time is normalized. By default a 10 KDa marker is used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
InternalReferenceDilution Factors
For each member of SamplesIn, indicates the factor by which the InternalReference needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[1]
InternalReferenceVolumes
For each member of SamplesIn, indicates the volume of InternalReference to be diluted by a factor equal to InternalReferenceDilutionFactor to reach working concentration in the final assay volume containing all other components (i.e., sample, SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ConcentratedSDSBuffers
For each member of SamplesIn, indicates the SDS Buffer concentrate used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedSDSBufferDilution Factors
For each member of SamplesIn, indicates the factor by which the ConcentratedSDSBuffer needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
ConcentratedSDSBufferVolumes
For each member of SamplesIn, indicates the volume of ConcentratedSDSBuffer to reach its working concentration in the final assay volume containing all other components (i.e., sample, internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
Diluents
For each member of SamplesIn, indicates the solution used to bring the assay tube to TotalVolume so that all components are at working concentration in the final assay volume containing all other components (i.e., sample, internal standard, SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SDSBuffers
For each member of SamplesIn, indicates the SDS Buffer used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SDSBufferVolumes
For each member of SamplesIn, indicates the volume of SDSBuffer to add to sample to reach working concentration in the final assay volume containing all other components (i.e., sample, internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ReducingAgents
For each member of SamplesIn, indicates the reducing agent, e.g., β-mercaptoethanol or Dithiothreitol, added to the sample to reduce disulfide bridges in proteins.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ReducingAgentTarget Concentrations
For each member of SamplesIn, indicates the final concentration of the ReducingAgent in the sample.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
ReducingAgentVolumes
For each member of SamplesIn, indicates the volume of the ReducingAgent added to the sample to reach working concentration in the final assay volume containing all other components (i.e., sample, internal standard, SDSBuffer and alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
AlkylatingAgents
For each member of SamplesIn, indicates the alkylating agent, e.g., Iodoacetamide, added to the sample to prevent protein fragmentation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
AlkylatingAgentTarget Concentrations
For each member of SamplesIn, indicates the final concentration of the AlkylatingAgent in the sample.
Format: Multiple
Class: Real
Unit: 1 millimolar
Programmatic Pattern: GreaterEqualP[0 Molar]
AlkylatingAgentVolumes
For each member of SamplesIn, indicates the volume of the AlkylatingAgent added the sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SedimentationSupernatant Volumes
For each member of SamplesIn, indicates the volume of supernatant that should be aliquoted to assay AssayContainer after denaturation and centrifugation.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
InternalReferenceStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the InternalReference after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ConcentratedSDSBufferStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the ConcentratedSDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
DiluentStorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the Diluent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SDSBufferStorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the SDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ReducingAgentStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the ReducingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AlkylatingAgentStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the AlkylatingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplePreparationPrimitives
A set of instructions specifying the preparation of samples, ladders, standards, and blanks for capillary gel Electrophoresis SDS experiments, including dilution in SDS buffer, spiking internal standards, and addition of any reducing and / or alkylating agents.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
SamplePreparationProtocol
The sample manipulation protocol used to prepare samples for capillary gel Electrophoresis SDS experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol, SampleManipulation]
PostIncubationTransferSM Primitives
A set of instructions specifying the transfer of samples, ladders, standards, and blanks from sample preparation plate to assay plate after incubation and centrifugation for capillary gel Electrophoresis SDS experiments.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
PostIncubationTransferSM Protocol
The sample manipulation protocol used to transfer of samples, ladders, standards, and blanks from sample preparation plate to assay plate after incubation and centrifugation for capillary gel Electrophoresis SDS experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol, SampleManipulation]
IncubationProtocol
The PCR protocol used to heat denature samples, ladders, Standards, and Blanks for capillary ELISA experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol, PCR]
CentrifugationProtocol
The centrifugation protocol used to remove any precipitates after denaturation in preparation for capillary gel Electrophoresis SDS experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
DataFile
The output data file generated at the conclusion of the experiment by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
CartridgeStorageCondition
The non-default storage condition for the Cartridge after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Instrument Setup
Cartridge
The capillary electrophoresis cartridge loaded on the instrument for Capillary gel Electrophoresis-SDS experiments. The cartridge holds a single capillary and the anode running buffer.
Format: Single
Class: Link
Programmatic Pattern: _Link
CartridgeInsert
The insert loaded on the Capillary gel Electrophoresis-SDS cartridge. The insert holds the top running buffer or the cleaning cartridge.
Format: Single
Class: Link
Programmatic Pattern: _Link
CartridgeInsertReplacement
The insert to be picked if needed, based on ReplaceCartridgeInsert.
Format: Single
Class: Link
Programmatic Pattern: _Link | Null
TopRunningBuffer
Indicates the buffer loaded onto the cartridge for separation. This buffer must be compatible with the cathode running buffer loaded on the instrument (see: OnBoardRunningBuffer).
Format: Single
Class: Link
Programmatic Pattern: _Link
TopRunningBufferBackup
Indicates the buffer loaded onto the cartridge for separation. This is a backup that will be picked only if a cartridge purge is required.
Format: Single
Class: Link
Programmatic Pattern: _Link
PurgeCartridge
Indicates whether cartridge should be purged even if not prompted by instrument.
Format: Single
Class: Expression
Programmatic Pattern: IfRequired | True | False
CartridgeTipCleanup
A beaker with water used to clean the capillary tip if needed after a run ends.
Format: Single
Class: Link
Programmatic Pattern: _Link
SampleTemperature
The sample tray temperature at which samples are maintained while awaiting injection.
Format: Single
Class: Integer
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
Caps
The caps used to seal reagent vials during a gel electrophoresis SDS experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersWithPressureCaps
Containers that are currently capped with pressure caps.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
ContainersWithClearCaps
Containers that are currently capped with clear caps.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
CleanupCartridgeCap
The caps used to seal reagent vials during a gel electrophoresis SDS experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
ConditioningAcid
The Conditioning Acid solution used to wash the capillary (every 12 injections).
Format: Single
Class: Link
Programmatic Pattern: _Link
ConditioningBase
The Conditioning Base solution used to wash the capillary (every 12 injections).
Format: Single
Class: Link
Programmatic Pattern: _Link
ConditioningWashSolution
The solution used to wash the capillary after acid and base wash (every 12 injections).
Format: Single
Class: Link
Programmatic Pattern: _Link
SeparationMatrix
The sieving matrix loaded onto the capillary before each sample for separation. The mash-like matrix slows the migration of proteins based on their size so that larger proteins migrate slower than smaller ones.
Format: Single
Class: Link
Programmatic Pattern: _Link
SystemWashSolution
The solution used to wash the capillary after conditioning and, separately, rinse the tip twice before every injection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PlaceholderContainer
The PlaceholderContainer is an empty vial used to dry the capillary after each wash.
Format: Single
Class: Link
Programmatic Pattern: _Link
BottomRunningBuffer
Indicates the buffer in which the capillary docks for separation. This buffer must be compatible with the anode running buffer loaded on the Capillary gel electrophoresis SDS cartridge (see: OnBoardRunningBuffer).
Format: Single
Class: Link
Programmatic Pattern: _Link
ConditioningAcidStorage Condition
The non-default storage condition for the ConditioningAcid once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ConditioningBaseStorage Condition
The non-default storage condition for the ConditioningBase once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SeparationMatrixStorage Condition
The non-default storage condition for the SeparationMatrix once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SystemWashSolutionStorage Condition
The non-default storage condition for the SystemWashSolution once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
PlateSeal
The adhesive film used to seal the assay plate once it is prepared for injection. The seal should be pierceable, if not, it should be removed before loading the plate to the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Cartridge Cleanup
CleanupCartridge
Indicates the item that is used to clean up the experiment cartridge after every run.
Format: Single
Class: Link
Programmatic Pattern: _Link
CleanupCartridgeWashSolution
Indicates the solution that is used to clean up the experiment cartridge after every run and its appropriate cap.
Format: Single
Class: Link
Programmatic Pattern: _Link
Separation
InjectionVoltageDuration Profiles
For each member of SamplesIn, indicates the series of voltages and durations to apply onto the capillary while docked in the sample to electroinject proteins in the sample into the matrix in the capillary.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
SeparationVoltageDuration Profiles
For each member of SamplesIn, indicates the series of voltages and durations to apply onto the capillary while docked in running buffer to separate proteins by molecular weight as they migrate through the separation matrix in the capillary.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Minute]}..}
Ladders
Ladders
A mixtures of known analytes that serve as reference for interpolation of the molecular weight of unknown samples in the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderStorageConditions
For each member of Ladders, indicates the non-default storage condition.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderAnalytes
For each member of Ladders, Indicates the Molecules in the ladder mixture.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectP[Model[Molecule]]..}
LadderAnalyteMolecularWeights
For each member of Ladders, indicates the molecular weights of analytes included in ladder.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterP[0 Kilodalton]..}
LadderAnalyteLabels
For each member of Ladders, indicates the label of each analyte included in ladder.
Format: Multiple
Class: Expression
Programmatic Pattern: {_String..}
LadderTotalVolumes
For each member of Ladders, indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a ladder, an InternalReference, and an SDSBuffer.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderDilutionFactors
For each member of Ladders, indicates the factor by which the ladder should be diluted by to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[1]
LadderVolumes
For each member of Ladders, indicates the volume of ladder required to reach its working concentration in LadderTotalVolume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderPremadeMasterMix Reagents
For each member of Ladders, indicates the premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderPremadeMasterMix Diluents
For each member of Ladders,indicates the solution used to dilute the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderPremadeMasterMixReagent DilutionFactors
For each member of Ladders, indicates the factor by which the premade mastermix should be diluted by in the final assay tube.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
LadderPremadeMasterMixVolumes
For each member of Ladders, indicates the volume of the premade mastermix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderInternalReferences
For each member of Ladders, indicates the internal standard stock solution added in sample preparation. The internal standard stock solution contains the analyte that serves as the reference by which Relative Migration Time is normalized. By default a 10 KDa marker is used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderInternalReference DilutionFactors
For each member of Ladders, indicates the factor by which the InternalReference needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
LadderInternalReference Volumes
For each member of Ladders, indicates the volume of InternalReference to be diluted by a factor equal to InternalReferenceDilutionFactor to reach working concentration in the final assay volume containing all other components (i.e., Ladder SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderConcentratedSDSBuffers
For each member of Ladders, indicates the SDS Buffer concentrate used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderConcentratedSDSBuffer DilutionFactors
For each member of Ladders, indicates the factor by which the ConcentratedSDSBuffer needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
LadderConcentratedSDSBuffer Volumes
For each member of Ladders, indicates the volume of ConcentratedSDSBuffer to reach its working concentration in the final assay volume containing all other components (i.e., Ladder internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderDiluents
For each member of Ladders, indicates the solution used to bring the assay tube to TotalVolume so that all components are at working concentration in the final assay volume containing all other components (i.e., Ladder internal standard, SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderSDSBuffers
For each member of Ladders, indicates the SDS Buffer used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderSDSBufferVolumes
For each member of Ladders, indicates the volume of SDSBuffer to add to Ladder to reach working concentration in the final assay volume containing all other components (i.e., Ladder internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderReducingAgents
For each member of Ladders, indicates the reducing agent, e.g., β-mercaptoethanol or Dithiothreitol, added to the Ladder to reduce disulfide bridges in proteins.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderReducingAgentTarget Concentrations
For each member of Ladders, indicates the final concentration of the ReducingAgent in the sample.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
LadderReducingAgentVolumes
For each member of Ladders, indicates the volume of the ReducingAgent added to the Ladder to reach working concentration in the final assay volume containing all other components (i.e., Ladder internal standard, SDSBuffer and alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderAlkylatingAgents
For each member of Ladders, indicates the alkylating agent, e.g., Iodoacetamide, added to the Ladder to prevent protein fragmentation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
LadderAlkylatingAgentTarget Concentrations
For each member of Ladders, indicates the final concentration of the AlkylatingAgent in the sample.
Format: Multiple
Class: Real
Unit: 1 millimolar
Programmatic Pattern: GreaterEqualP[0 Molar]
LadderAlkylatingAgentVolumes
For each member of Ladders, indicates the volume of the AlkylatingAgent added the sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderSedimentation SupernatantVolumes
For each member of Ladders, indicates the volume of supernatant that should be aliquoted to assay AssayContainer after denaturation and centrifugation.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
LadderInternalReference StorageConditions
For each member of Ladders, indicates the non-default storage condition for the InternalReference after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderConcentratedSDSBuffer StorageConditions
For each member of Ladders, indicates the non-default storage condition for the ConcentratedSDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderDiluentStorage Conditions
For each member of Ladders, indicates the non-default storage condition for the Diluent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderSDSBufferStorage Conditions
For each member of Ladders, indicates the non-default storage condition for the SDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderReducingAgentStorage Conditions
For each member of Ladders, indicates the non-default storage condition for the ReducingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderAlkylatingAgentStorage Conditions
For each member of Ladders, indicates the non-default storage condition for the AlkylatingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
LadderInjectionVoltage DurationProfiles
For each member of Ladders, indicates the series of voltages and durations to apply onto the capillary while sampling Ladders for separation.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
LadderSeparationVoltage DurationProfiles
For each member of Ladders, indicates the series of voltages and durations to apply onto the capillary while docked in running buffer to separate proteins by molecular weight as they migrate through the separation matrix in the capillary.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Minute]}..}
Standards
Standards
Known analytes that serve as standards for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardStorageConditions
For each member of Standards, indicates the non-default storage condition.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardTotalVolumes
For each member of Standards, indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains Standard InternalReference, SDSBuffer, and ReducingAgent and/or AlkylatingAgent.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardVolumes
For each member of Standards, indicates the volume of blank required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardPremadeMasterMix Reagents
For each member of Standards, indicates the premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardPremadeMasterMix Diluents
For each member of Standards,indicates the solution used to dilute the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardPremadeMasterMix ReagentDilutionFactors
For each member of Standards, indicates the factor by which the premade mastermix should be diluted by in the final assay tube.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
StandardPremadeMasterMix Volumes
For each member of Standards, indicates the volume of the premade mastermix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardInternalReferences
For each member of Standards, indicates the internal standard stock solution added in sample preparation. The internal standard stock solution contains the analyte that serves as the reference by which Relative Migration Time is normalized. By default a 10 KDa marker is used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardInternalReference DilutionFactors
For each member of Standards, indicates the factor by which the InternalReference needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
StandardInternalReference Volumes
For each member of Standards, indicates the volume of InternalReference to be diluted by a factor equal to InternalReferenceDilutionFactor to reach working concentration in the final assay volume containing all other components (i.e., Standard SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardConcentratedSDS Buffers
For each member of Standards, indicates the SDS Buffer concentrate used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardConcentratedSDSBuffer DilutionFactors
For each member of Standards, indicates the factor by which the ConcentratedSDSBuffer needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
StandardConcentratedSDSBuffer Volumes
For each member of Standards, indicates the volume of ConcentratedSDSBuffer to reach its working concentration in the final assay volume containing all other components (i.e., Standard internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDiluents
For each member of Standards, indicates the solution used to bring the assay tube to TotalVolume so that all components are at working concentration in the final assay volume containing all other components (i.e., Standard internal standard, SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardSDSBuffers
For each member of Standards, indicates the SDS Buffer used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardSDSBufferVolumes
For each member of Standards, indicates the volume of SDSBuffer to add to Standard to reach working concentration in the final assay volume containing all other components (i.e., Standard internal standard, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardReducingAgents
For each member of Standards, indicates the reducing agent, e.g., β-mercaptoethanol or Dithiothreitol, added to the Standard to reduce disulfide bridges in proteins.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardReducingAgentTarget Concentrations
For each member of Standards, indicates the final concentration of the ReducingAgent in the sample.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
StandardReducingAgentVolumes
For each member of Standards, indicates the volume of the ReducingAgent added to the Standard to reach working concentration in the final assay volume containing all other components (i.e., Standard internal standard, SDSBuffer and alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardAlkylatingAgents
For each member of Standards, indicates the alkylating agent, e.g., Iodoacetamide, added to the Standard to prevent protein fragmentation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardAlkylatingAgentTarget Concentrations
For each member of Standards, indicates the final concentration of the AlkylatingAgent in the sample.
Format: Multiple
Class: Real
Unit: 1 millimolar
Programmatic Pattern: GreaterEqualP[0 Molar]
StandardAlkylatingAgent Volumes
For each member of Standards, indicates the volume of the AlkylatingAgent added the sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardSedimentation SupernatantVolumes
For each member of Standards, indicates the volume of supernatant that should be aliquoted to assay AssayContainer after denaturation and centrifugation.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardInternalReference StorageConditions
For each member of Standards, indicates the non-default storage condition for the InternalReference after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardConcentratedSDSBuffer StorageConditions
For each member of Standards, indicates the non-default storage condition for the ConcentratedSDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardDiluentStorage Conditions
For each member of Standards, indicates the non-default storage condition for the Diluent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardSDSBufferStorage Conditions
For each member of Standards, indicates the non-default storage condition for the SDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardReducingAgentStorage Conditions
For each member of Standards, indicates the non-default storage condition for the ReducingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardAlkylatingAgent StorageConditions
For each member of Standards, indicates the non-default storage condition for the AlkylatingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardInjectionVoltage DurationProfiles
For each member of Standards, indicates the series of voltages and durations to apply onto the capillary while sampling Standards for separation.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
StandardSeparationVoltage DurationProfiles
For each member of Standards, indicates the series of voltages and durations to apply onto the capillary while docked in running buffer to separate proteins by molecular weight as they migrate through the separation matrix in the capillary.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Minute]}..}
Blanks
Blanks
Known analytes that serve as Blanks for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankStorageConditions
For each member of Blanks, indicates the non-default storage condition.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankTotalVolumes
For each member of Blanks, indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains Blank, InternalReference, SDSBuffer, and ReducingAgent and/or AlkylatingAgent.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankVolumes
For each member of Blanks, indicates the volume of blank required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankPremadeMasterMixReagents
For each member of Blanks, indicates the premade master mix used for CESDS experiment, containing an SDS buffer, an internal Blank, and reducing and / or alkylating agents (if applicable).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankPremadeMasterMixDiluents
For each member of Blanks,indicates the solution used to dilute the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankPremadeMasterMixReagent DilutionFactors
For each member of Blanks, indicates the factor by which the premade mastermix should be diluted by in the final assay tube.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
BlankPremadeMasterMixVolumes
For each member of Blanks, indicates the volume of the premade mastermix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankInternalReferences
For each member of Blanks, indicates the internal Blank stock solution added in sample preparation. The internal Blank stock solution contains the analyte that serves as the reference by which Relative Migration Time is normalized. By default a 10 KDa marker is used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankInternalReference DilutionFactors
For each member of Blanks, indicates the factor by which the InternalReference needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
BlankInternalReferenceVolumes
For each member of Blanks, indicates the volume of InternalReference to be diluted by a factor equal to InternalReferenceDilutionFactor to reach working concentration in the final assay volume containing all other components (i.e., Blank SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankConcentratedSDSBuffers
For each member of Blanks, indicates the SDS Buffer concentrate used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankConcentratedSDSBuffer DilutionFactors
For each member of Blanks, indicates the factor by which the ConcentratedSDSBuffer needs to be diluted to reach working concentration.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
BlankConcentratedSDSBuffer Volumes
For each member of Blanks, indicates the volume of ConcentratedSDSBuffer to reach its working concentration in the final assay volume containing all other components (i.e., Blank internal Blank, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankDiluents
For each member of Blanks, indicates the solution used to bring the assay tube to TotalVolume so that all components are at working concentration in the final assay volume containing all other components (i.e., Blank internal Blank, SDS buffer, and reducing/alkylating agents).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankSDSBuffers
For each member of Blanks, indicates the SDS Buffer used to dilute and denature the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankSDSBufferVolumes
For each member of Blanks, indicates the volume of SDSBuffer to add to Blank to reach working concentration in the final assay volume containing all other components (i.e., Blank internal Blank, and reducing/alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankReducingAgents
For each member of Blanks, indicates the reducing agent, e.g., β-mercaptoethanol or Dithiothreitol, added to the Blank to reduce disulfide bridges in proteins.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankReducingAgentTarget Concentrations
For each member of Blanks, indicates the final concentration of the ReducingAgent in the sample.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
BlankReducingAgentVolumes
For each member of Blanks, indicates the volume of the ReducingAgent added to the Blank to reach working concentration in the final assay volume containing all other components (i.e., Blank internal Blank, SDSBuffer and alkylating agents).
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankAlkylatingAgents
For each member of Blanks, indicates the alkylating agent, e.g., Iodoacetamide, added to the Blank to prevent protein fragmentation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankAlkylatingAgentTarget Concentrations
For each member of Blanks, indicates the final concentration of the AlkylatingAgent in the sample.
Format: Multiple
Class: Real
Unit: 1 millimolar
Programmatic Pattern: GreaterEqualP[0 Molar]
BlankAlkylatingAgentVolumes
For each member of Blanks, indicates the volume of the AlkylatingAgent added the sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankSedimentationSupernatant Volumes
For each member of Blanks, indicates the volume of supernatant that should be aliquoted to assay AssayContainer after denaturation and centrifugation.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankInternalReferenceStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the InternalReference after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankConcentratedSDSBuffer StorageConditions
For each member of Blanks, indicates the non-default storage condition for the ConcentratedSDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankDiluentStorageConditions
For each member of Blanks, indicates the non-default storage condition for the Diluent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankSDSBufferStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the SDSBuffer after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankReducingAgentStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the ReducingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankAlkylatingAgentStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the AlkylatingAgent after samples are transferred to assay tubes.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankInjectionVoltageDuration Profiles
For each member of Blanks, indicates the series of voltages and durations to apply onto the capillary while sampling Blanks for separation.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
BlankSeparationVoltage DurationProfiles
For each member of Blanks, indicates the series of voltages and durations to apply onto the capillary while docked in running buffer to separate proteins by molecular weight as they migrate through the separation matrix in the capillary.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Minute]}..}
Last modified on Mon 26 Sep 2022 15:43:49