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Object[Protocol, SupercriticalFluidChromatography]

A protocol describing the separation of materials using supercritical CO2 fluid chromatography (SFC).

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Protocol, SupercriticalFluidChromatography, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].

    Author

    The investigator who created this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

General

    DateConfirmed

    The date on which the protocol first entered processing or a backlog.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateEnqueued

    The date on which the protocol was accepted and added to the queue.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateStarted

    The date on which the protocol moved from the front of the queue to begin running in the lab.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateCompleted

    The date on which the last step of this protocol's execution was finished.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateCanceled

    The date on which the protocol was canceled and removed from the queue.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    Script

    The script that created this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    SubprotocolScripts

    All scripts generated by this protocol as a list of subprotocols.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    Site

    The ECL site at which this protocol was executed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    SamplesIn

    Input samples for this analytical or preparative experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ContainersIn

    Any containers containing this protocols' SamplesIn.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    NumberOfReplicates

    Indicates the number of times the experiment is repeated with identical parameters.
    Format: Single
    Class: Integer
    Programmatic Pattern: GreaterP[0, 1]

    Status

    The current status of the protocol.
    Format: Single
    Class: Expression
    Programmatic Pattern: ProtocolStatusP

    CheckpointProgress

    A listing of all the checkpoints passed in the execution of this protocol.
    Format: Multiple

    [[1]] Name

      Header: Name
      Class: String
      Programmatic Pattern: _String

    [[2]] Start Time

      Header: Start Time
      Class: Date
      Programmatic Pattern: _?DateObjectQ

    [[3]] End Time

      Header: End Time
      Class: Date
      Programmatic Pattern: _?DateObjectQ|Null

    SubprotocolDescription

    A short title indicating what this subprotocol accomplishes.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    BatchedUnitOperations

    The individual batches of the protocol, as they will be executed in the lab.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ReplacementProtocol

    If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    Sterile

    Indicates if the protocol is performed in a sterile environment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    LiquidHandlingLog

    The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    PooledSamplesIn

    The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}

    NestedIndexMatchingSamplesIn

    The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}

    InitialNitrogenPressure

    The pressure data of the nitrogen gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    NitrogenPressureLog

    The pressure log for the nitrogen gas source for the relevant section of the facility.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCO2Pressure

    The pressure data of the CO2 gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CO2PressureLog

    The pressure log for the CO2 gas source for the relevant section of the facility.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialArgonPressure

    The pressure data of the argon gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    HoldOrder

    Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    Priority

    Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    StartDate

    The date at which this protocol should be prioritized to start.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    Branch

    The git branch that this protocol should be run on.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    Instrument

    The device containing a pump, column oven, and detector(s) that executes this experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    Scale

    Indicates if the SFC is intended to separate material (Preparative) and therefore collect fractions and/or analyze properties of the material (Analytical).
    Format: Single
    Class: Expression
    Programmatic Pattern: PurificationScaleP

    SeparationMode

    The type of chromatographic separation that categorizes the mobile and stationary phase used, ideally for optimal sample separation and resolution.
    Format: Single
    Class: Expression
    Programmatic Pattern: SeparationModeP

    Detectors

    Indicates the types of measurements performed for the experiment and available on the Instrument.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: ChromatographyDetectorTypeP

    AcquisitionMode

    For experiments with MassSpectrometry available, the type of acquisition being performed in this protocol. MS mode measures the masses of the intact analytes, whereas MSMS measures the masses of the analytes fragmented by collision-induced dissociation.
    Format: Single
    Class: Expression
    Programmatic Pattern: AcquisitionModeP

    MassAnalyzer

    For experiments with MassSpectrometry available, the type of the component of the mass spectrometer that performs ion separation based on m/z (mass-to-charge ratio). SingleQuadrupole selects ions individually for measurement.
    Format: Single
    Class: Expression
    Programmatic Pattern: MassAnalyzerTypeP

    IonSource

    For experiments with MassSpectrometry available, the type of ionization used to create gas-phase ions from the molecules in the sample. Electrospray ionization (ESI) produces ions using an electrospray in which a high voltage is applied to a liquid to create an aerosol, and gas-phase ions are formed from the fine spray of charged droplets as a result of solvent evaporation and Coulomb fission.
    Format: Single
    Class: Expression
    Programmatic Pattern: IonSourceP

    PurgeWasteContainerA

    The container used to hold waste from air bubble removal procedures for buffer lines. It is also used to hold waste from rinsing plumbing connections.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    PurgeWasteContainerB

    The container used to hold waste from air bubble removal procedures for buffer lines.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventA

    The solvent connected to position A, the flow of which is directed by GradientA compositions.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventB

    The solvent connected to position B, the flow of which is directed by GradientB compositions.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventC

    The solvent connected to position C, the flow of which is directed by GradientC compositions.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventD

    The solvent connected to position D, the flow of which is directed by GradientD compositions.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MakeupSolvent

    The solution pumped and supplementing the column effluent on entry to the mass spectrometer.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventACap

    The cap used to aspirate CosolventA during this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventBCap

    The cap used to aspirate CosolventB during this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventCCap

    The cap used to aspirate CosolventC during this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CosolventDCap

    The cap used to aspirate CosolventD during this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MakeupSolventCap

    The cap used to aspirate MakeupSolvent during this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCosolventAVolume

    The volume of CosolventA immediately before the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InitialCosolventBVolume

    The volume of CosolventB immediately before the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InitialCosolventCVolume

    The volume of CosolventC immediately before the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InitialCosolventDVolume

    The volume of CosolventD immediately before the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InitialMakeupSolventVolume

    The volume of MakeupSolvent immediately before the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InitialCosolventAAppearance

    An image of CosolventA taken immediately before the experiment was started.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCosolventBAppearance

    An image of CosolventB taken immediately before the experiment was started.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCosolventCAppearance

    An image of CosolventC taken immediately before the experiment was started.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCosolventDAppearance

    An image of CosolventD taken immediately before the experiment was started.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialMakeupSolvent Appearance

    An image of MakeupSolvent taken immediately before the experiment was started.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MaxAcceleration

    The maximum rate at which it's safe to increase the flow rate for the column and instrument during the run.
    Format: Single
    Class: Real
    Unit: 1 milliliter per minute squared
    Programmatic Pattern: 0 Milli Liter ------------- Minute Hold[GreaterEqualP[-------------]] Minute

    InjectionTable

    The sequence of samples injected for a given experiment run including for ColumnPrime, SamplesIn, Standards, Blanks, and ColumnFlush.
    Format: Multiple

    [Type]

      Header: Type
      Class: Expression

    [Sample]

    [InjectionVolume]

      Header: InjectionVolume
      Class: Real
      Unit: 1 microliter

    [Column]

    [Gradient]

    [DilutionFactor]

      Header: DilutionFactor
      Class: Real

    [ColumnTemperature]

      Header: ColumnTemperature
      Class: Real
      Unit: 1 degree Celsius

    [Data]

Replicate Experiments

    Template

    The protocol whose option selections should be selected as the default options for this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    ProtocolsTemplated

    Protocols which were generated using this protocol's options as a starting point for its option defaults.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Sample Preparation

    PreparatoryUnitOperations

    A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: _ | _

    PreparatoryPrimitives

    A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: _ | _

    VolumeCheckSamplePrep

    Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    IncubateSamplePreparation

    For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    CentrifugeSamplePreparation

    For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    FilterSamplePreparation

    For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Filtration]

      Header: Filtration
      Class: Boolean

    [FiltrationType]

      Header: FiltrationType
      Class: Expression

    [Filter]

    [FilterMaterial]

      Header: FilterMaterial
      Class: Expression

    [FilterPoreSize]

      Header: FilterPoreSize
      Class: Real
      Unit: 1 micrometer

    [FilterContainerOut]

      Header: FilterContainerOut
      Class: Expression

    [FilterInstrument]

    [FilterSyringe]

    [FilterHousing]

    [FilterIntensity]

      Header: FilterIntensity
      Class: VariableUnit

    [FilterTime]

      Header: FilterTime
      Class: Real
      Unit: 1 minute

    [FilterTemperature]

      Header: FilterTemperature
      Class: Real
      Unit: 1 degree Celsius

    [FilterSterile]

      Header: FilterSterile
      Class: Boolean

    [FilterAliquotContainer]

      Header: FilterAliquotContainer
      Class: Expression

    [FilterAliquot]

      Header: FilterAliquot
      Class: Real
      Unit: 1 liter

    [PrefilterMaterial]

      Header: PrefilterMaterial
      Class: Expression

    [PrefilterPoreSize]

      Header: PrefilterPoreSize
      Class: Real
      Unit: 1 micrometer

    [FilterAliquotDestinationWell]

      Header: FilterAliquotDestinationWell
      Class: Expression

    SamplePreparationProtocols

    Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    PooledIncubateSample Preparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    NestedIndexMatchingIncubate SamplePreparation

    For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    PooledCentrifugeSample Preparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    NestedIndexMatchingCentrifuge SamplePreparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    FilteredSamples

    Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample]

    Calibrant

    A sample with known m/z (mass-to-charge ratios) used to calibrate the mass spectrometer.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    SampleTemperature

    The nominal temperature of the autosampler where the input samples are incubated prior to injection on the column.
    Format: Single
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    SampleVolumes

    For each member of SamplesIn, the volume taken from the sample and injected onto the column.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Micro Liter]

    PlateSeal

    The package of piercable, adhesive film used to cover plates of injection samples in this experiment in order to mitigate sample evaporation.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

Aliquoting

    AliquotSamplePreparation

    For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
    Format: Multiple

    [Aliquot]

      Header: Aliquot
      Class: Boolean

    [AliquotAmount]

      Header: AliquotAmount
      Class: Expression

    [TargetConcentration]

      Header: TargetConcentration
      Class: Expression

    [AssayVolume]

      Header: AssayVolume
      Class: Real
      Unit: 1 milliliter

    [AliquotContainer]

      Header: AliquotContainer
      Class: Expression

    [AssayBuffer]

    [BufferDiluent]

    [BufferDilutionFactor]

      Header: BufferDilutionFactor
      Class: Real

    [ConcentratedBuffer]

    [DestinationWell]

      Header: DestinationWell
      Class: Expression

    [TargetConcentrationAnalyte]

      Header: TargetConcentrationAnalyte
      Class: Expression

    [AliquotSampleLabel]

      Header: AliquotSampleLabel
      Class: String

    AliquotProtocols

    Protocols used to create AliquotSamples prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    AliquotPreparationProtocols

    Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    AliquotSamples

    Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample]

    AliquotContainers

    Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SaveAliquots

    Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    ConsolidateAliquots

    Indicates if identical aliquots should be consolidated in a single sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    AliquotPreparation

    Indicates if the aliquotting should occur manually or on a robotic liquid handler.
    Format: Single
    Class: Expression
    Programmatic Pattern: PreparationMethodP

    AliquotVolumes

    For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterP[0 Micro Liter]

    AliquotMasses

    For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 milligram
    Programmatic Pattern: GreaterP[0 Milligram]

    AliquotAmounts

    For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: VariableUnit
    Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]

    TargetConcentrations

    For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milligram Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]] Milliliter

    AssayVolumes

    For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Micro Liter]

    AssayBuffers

    For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ConcentratedBuffers

    For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    BufferDilutionFactors

    For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Real
    Programmatic Pattern: GreaterP[0]

    BufferDiluents

    For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ConcentratedBuffer

    The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Integer
    Programmatic Pattern: GreaterP[0]

    BufferDiluent

    The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    AssayBuffer

    The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

Sample Post-Processing

    PostProcessingProtocols

    Protocols used to perform post-processing such as sample imaging or volume measurement.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ImageSample

    Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    MeasureVolume

    Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    MeasureWeight

    Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    Measure

    Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    Storage

    Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
    Format: Single
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Experimental Results

    Data

    Any primary data generated by this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SamplesOut

    Any preparative samples generated by the protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ContainersOut

    Any containers holding SamplesOut generated by this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    EnvironmentalData

    Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    StandardData

    The chromatography traces generated for the standard's injection.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    BlankData

    The chromatography traces generated for the blank's injection.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    PrimeData

    Chromatography traces generated for any column prime runs.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    FlushData

    Chromatography traces generated for any column flush runs.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    InitialAnalyteVolumes

    The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) measured prior to injection.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalAnalyteVolumes

    The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) measured at the end of the protocol.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    InjectedAnalyteVolumes

    The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) that was injected during the protocol, calculated as the difference between InitialAnalyteVolumes and FinalAnalyteVolumes.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Liter]

Troubleshooting

    TroubleshootingReports

    Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Resources

    AwaitingResources

    Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    ShippingMaterials

    Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
    Format: Multiple

    [[1]] Transaction

    [[2]] Required Product

    HazardousWaste

    The types of waste generated by this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    DispenserContainers

    Containers gathered as destinations for any resources obtained from a chemical dispenser.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    GasSources

    A list of containers that supplied gas to instruments in this protocol through plumbing connections.
    Format: Multiple

    [[1]] Gas Type

      Header: Gas Type
      Class: Expression
      Programmatic Pattern: GasP

    [[2]] Source Container

      Header: Source Container
      Class: Link
      Programmatic Pattern: _Link

    [[3]] Supplied Instrument

      Header: Supplied Instrument
      Class: Link
      Programmatic Pattern: _Link

Cleaning

    WasteGenerated

    For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
    Format: Multiple

    [Waste]

    [Weight]

      Header: Weight
      Class: Real
      Unit: 1 gram

    ExternalNeedleWashSolution

    The solution used to wash the outside of the injection needle and pumps before, during, and after the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    NeedleWashSolution

    The solution used to wash the inside of the injection needle and pumps before, during, and after the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

Storage Information

    StoragePrice

    The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
    Format: Single
    Class: Real
    Unit: 1 US dollar per month
    Programmatic Pattern: USD Hold[GreaterEqualP[0 -----]] Month

Sample Storage

    SamplesInStorage

    For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

    AliquotStorage

    For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

    SamplesOutStorage

    For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Option Handling

    ResolvedOptions

    The final options used as parameters for this protocol, after automatic options are resolved.
    Format: Single
    Class: Compressed
    Programmatic Pattern: {_Rule...}

    UnresolvedOptions

    The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
    Format: Single
    Class: Compressed
    Programmatic Pattern: {_Rule...}

Computations

    ParallelComputations

    List of computational jobs generated and run in parallel to the execution of this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ComputationsOutstanding

    Indicates if any of the computation jobs in ParallelComputations have not yet completed.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    ErroneousComputations

    List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Column Installation

    ColumnSelection

    The stationary phase device(s) through which the Cosolvents and input samples flow. It adsorbs and separates the molecules within the sample based on the properties of the Cosolvents, samples, column material, and ColumnTemperature.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ColumnOrientation

    For each member of ColumnSelection, the direction of the Column for the protocol with respect to the flow.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: ColumnOrientationP

Column Prime

    ColumnPrimeGradientMethods

    For each member of ColumnSelection, the composition of the CO2 and cosolvents within the flow, defined for specific time points during the equilibration of the columns (column prime).
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Method]

    ColumnPrimeCO2Gradient

    For each member of ColumnSelection, the percentage of supercritical CO2 in the composition over time, in the form: {Time, % CO2} or a single % CO2 for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnPrimeGradientA

    For each member of ColumnSelection, the percentage of CosolventA in the composition over time, in the form: {Time, % Cosolvent A} or a single % Cosolvent A for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnPrimeGradientB

    For each member of ColumnSelection, the percentage of CosolventB in the composition over time, in the form: {Time, % Cosolvent B} or a single % Cosolvent B for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnPrimeGradientC

    For each member of ColumnSelection, the percentage of CosolventC in the composition over time, in the form: {Time, % Cosolvent C} or a single % Cosolvent C for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnPrimeGradientD

    For each member of ColumnSelection, the percentage of CosolventD in the composition over time, in the form: {Time, % Cosolvent D} or a single % Cosolvent D for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnPrimeBackPressure

    For each member of ColumnSelection, the applied pressure between the atmosphere and the PhotoDiodeArray detector exit, in the form: {Time, BackPressure} or a single BackPressure for each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], GreaterP[0 PSI]}...} | GreaterP[0 PSI]

    ColumnPrimeFlowRates

    For each member of ColumnSelection, the total rate of mobile phase pumped through the instrument for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    ColumnPrimeTemperatures

    For each member of ColumnSelection, the nominal temperature of the column compartment for each column prime run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    ColumnPrimeIonModes

    For each member of ColumnSelection, indicates if positively or negatively charged ions are analyzed each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IonModeP

    ColumnPrimeMakeupFlowRates

    For each member of ColumnSelection, the total rate of MakeupSolvent pumped through the instrument for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    ColumnPrimeMassSelections

    For each member of ColumnSelection, the all of the mass-to-charge ratio (m/z) measured via mass spectrometry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: 0 Gram Hold[{GreaterP[------]..}] Mole

    ColumnPrimeMinMasses

    For each member of ColumnSelection, the lowest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterEqualP[------]] Mole

    ColumnPrimeMaxMasses

    For each member of ColumnSelection, the highest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterP[------]] Mole

    ColumnPrimeProbeTemperatures

    For each member of ColumnSelection, the temperature of the needle that sprays column effluent into the ionization chamber for each ColumnPrime run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    ColumnPrimeSourceTemperatures

    For each member of ColumnSelection, the temperature that the source block is set to in order to discourage condensation and decrease solvent clustering in the reduced vacuum region of the source. The source block is the metallic chamber with reduced pressure separating the sprayer (at atmospheric pressure) and the inside of the mass spectrometer (at 10^-7 Torr of pressure). It consists of the sampling cone, the isolation valve, and the ion block. The source temperature setting affects sensitivity.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    ColumnPrimeESICapillary Voltages

    For each member of ColumnSelection, the applied voltage differential between the injector and the inlet plate for the mass spectrometry in order to ionize analyte molecules.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    ColumnPrimeSamplingCone Voltages

    For each member of ColumnSelection, the voltage offset between the 1st and 2nd stage of the stepwave ion guide which leads ions coming from the sample cone (the metal shield covering the front of the source block), towards the quadrupole mass analyzer.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    ColumnPrimeScanTimes

    For each member of ColumnSelection, the duration of time allowed to pass between each spectral acquisition.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    ColumnPrimeMassDetectionGains

    For each member of ColumnSelection, indicates the arbitrary-scaled signal amplification of the mass spectrometry measurement.
    Format: Multiple
    Class: Real
    Programmatic Pattern: RangeP[0.01, 1000., 0.01]

    ColumnPrimeAbsorbance Selection

    For each member of ColumnSelection, all the wavelengths of light absorbed in the detector's flow cell for a PhotoDiodeArray (PDA) detector for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterP[0 Centimeter]..}

    ColumnPrimeMinAbsorbance Wavelengths

    For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnPrimeMaxAbsorbance Wavelengths

    For each member of ColumnSelection, the maximum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnPrimeWavelength Resolutions

    For each member of ColumnSelection, the increment in wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnPrimeUVFilter

    For each member of ColumnSelection, indicates whether or not to block UV wavelengths (less than 210 nm) from being transmitted through the sample for PDA detector.
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    ColumnPrimeAbsorbanceSampling Rates

    For each member of ColumnSelection, indicates the frequency of measurement for UVVis or PDA detectors.
    Format: Multiple
    Class: Real
    Unit: 1 reciprocal second
    Programmatic Pattern: 1 Hold[GreaterP[0 ------]] Second

Gradient

    GradientMethods

    For each member of SamplesIn, the CO2 and solvent gradient used for purification.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Method]

    CO2Gradient

    For each member of SamplesIn, the percentage of supercritical CO2 in the composition over time, in the form: {Time, % CO2} or a single % CO2 for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    GradientA

    For each member of SamplesIn, the percentage of CosolventA in the composition over time, in the form: {Time, % Cosolvent A} or a single % Cosolvent A for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    GradientB

    For each member of SamplesIn, the percentage of CosolventB in the composition over time, in the form: {Time, % Cosolvent B} or a single % Cosolvent B for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    GradientC

    For each member of SamplesIn, the percentage of CosolventC in the composition over time, in the form: {Time, % Cosolvent C} or a single % Cosolvent C for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    GradientD

    For each member of SamplesIn, the percentage of CosolventD in the composition over time, in the form: {Time, % Cosolvent D} or a single % Cosolvent D for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BackPressures

    For each member of SamplesIn, the applied pressure between the atmosphere and the PhotoDiodeArray detector exit, in the form: {Time, BackPressure} or a single BackPressure for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], GreaterP[0 PSI]}...} | GreaterP[0 PSI]

    FlowRate

    For each member of SamplesIn, the total rate of mobile phase pumped through the instrument.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    Columns

    For each member of SamplesIn, the column used from the column selector for the separation and measurement.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ColumnTemperatures

    For each member of SamplesIn, the nominal temperature of the column compartment during a run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    FinalCosolventAVolume

    The volume of CosolventA immediately after the experiment was completed.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalCosolventBVolume

    The volume of CosolventB immediately after the experiment was completed.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalCosolventCVolume

    The volume of CosolventC immediately after the experiment was completed.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalCosolventDVolume

    The volume of CosolventD immediately after the experiment was completed.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalMakeupSolventVolume

    The volume of MakeupSolvent immediately after the experiment was started.
    Format: Single
    Class: Real
    Unit: 1 liter
    Programmatic Pattern: GreaterEqualP[0 Liter]

    FinalCosolventAAppearance

    An image of CosolventA taken immediately after the experiment was completed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FinalCosolventBAppearance

    An image of CosolventB taken immediately after the experiment was completed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FinalCosolventCAppearance

    An image of CosolventC taken immediately after the experiment was completed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FinalCosolventDAppearance

    An image of CosolventD taken immediately after the experiment was completed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FinalMakeupSolventAppearance

    An image of MakeupSolvent taken immediately after the experiment was completed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

Detection

    IonModes

    For each member of SamplesIn, indicates if positively or negatively charged ions are analyzed each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IonModeP

    MakeupFlowRates

    For each member of SamplesIn, the total rate of MakeupSolvent pumped through the instrument for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    MassSelection

    For each member of SamplesIn, all of the mass-to-charge ratio (m/z) measured via mass spectrometry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: 0 Gram Hold[{GreaterP[------]..}] Mole

    MinMasses

    For each member of SamplesIn, the lowest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterEqualP[------]] Mole

    MaxMasses

    For each member of SamplesIn, the highest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterP[------]] Mole

    ProbeTemperatures

    For each member of SamplesIn, the temperature of the needle that sprays column effluent into the ionization chamber.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    SourceTemperatures

    For each member of SamplesIn, the temperature that the source block is set to in order to discourage condensation and decrease solvent clustering in the reduced vacuum region of the source. The source block is the metallic chamber with reduced pressure separating the sprayer (at atmospheric pressure) and the inside of the mass spectrometer (at 10^-7 Torr of pressure). It consists of the sampling cone, the isolation valve, and the ion block. The source temperature setting affects sensitivity.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    ESICapillaryVoltages

    For each member of SamplesIn, the applied voltage differential between the injector and the inlet plate for the mass spectrometry in order to ionize analyte molecules.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    SamplingConeVoltages

    For each member of SamplesIn, the voltage offset between the 1st and 2nd stage of the stepwave ion guide which leads ions coming from the sample cone (the metal shield covering the front of the source block), towards the quadrupole mass analyzer.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    ScanTimes

    For each member of SamplesIn, the duration of time allowed to pass between each spectral acquisition.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    MassDetectionGains

    For each member of SamplesIn, indicates the arbitrary-scaled signal amplification of the mass spectrometry measurement.
    Format: Multiple
    Class: Real
    Programmatic Pattern: RangeP[0.01, 1000., 0.01]

    AbsorbanceSelection

    For each member of SamplesIn, all the wavelengths of light absorbed in the detector's flow cell for a PhotoDiodeArray (PDA) detector.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterP[0 Centimeter]..}

    MinAbsorbanceWavelengths

    For each member of SamplesIn, the minimum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    MaxAbsorbanceWavelengths

    For each member of SamplesIn, the maximum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    WavelengthResolutions

    For each member of SamplesIn, the increment in wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    UVFilters

    For each member of SamplesIn, indicates whether or not to block UV wavelengths (less than 210 nm) from being transmitted through the sample for Absorbance detectors (PDA).
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    AbsorbanceSamplingRates

    For each member of SamplesIn, indicates the frequency of measurement for PDA detectors.
    Format: Multiple
    Class: Real
    Unit: 1 reciprocal second
    Programmatic Pattern: 1 Hold[GreaterP[0 ------]] Second

Standards

    Standards

    Samples with known profiles used to calibrate peak integrations and retention times for a given run.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    StandardSampleVolumes

    For each member of Standards, the volume taken from the standard and injected onto the column.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Micro Liter]

    StandardGradientMethods

    For each member of Standards, the method used to describe the gradient used for purification.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Method]

    StandardCO2Gradient

    For each member of Standards, the percentage of supercritical CO2 in the composition over time, in the form: {Time, % CO2} or a single % CO2 for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    StandardGradientA

    For each member of Standards, the percentage of CosolventA in the composition over time, in the form: {Time, % Cosolvent A} or a single % Cosolvent A for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    StandardGradientB

    For each member of Standards, the percentage of CosolventB in the composition over time, in the form: {Time, % Cosolvent B} or a single % Cosolvent B for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    StandardGradientC

    For each member of Standards, the percentage of CosolventC in the composition over time, in the form: {Time, % Cosolvent C} or a single % Cosolvent C for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    StandardGradientD

    For each member of Standards, the percentage of CosolventD in the composition over time, in the form: {Time, % Cosolvent D} or a single % Cosolvent D for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    StandardBackPressures

    For each member of Standards, the applied pressure between the atmosphere and the PhotoDiodeArray detector exit, in the form: {Time, BackPressure} or a single BackPressure for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], GreaterP[0 PSI]}...} | GreaterP[0 PSI]

    StandardFlowRates

    For each member of Standards, the total rate of mobile phase pumped through the instrument.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    StandardColumns

    For each member of Standards, the column used from the column selector for the separation and measurement.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    StandardColumnTemperatures

    For each member of Standards, the nominal temperature of the column compartment during a run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    StandardIonModes

    For each member of Standards, indicates if positively or negatively charged ions are analyzed each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IonModeP

    StandardMakeupFlowRates

    For each member of Standards, the total rate of MakeupSolvent pumped through the instrument for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    StandardMassSelection

    For each member of Standards, all of the mass-to-charge ratio (m/z) measured via mass spectrometry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: 0 Gram Hold[{GreaterP[------]..}] Mole

    StandardMinMasses

    For each member of Standards, the lowest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterEqualP[------]] Mole

    StandardMaxMasses

    For each member of Standards, the highest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterP[------]] Mole

    StandardProbeTemperatures

    For each member of Standards, the temperature of the needle that sprays column effluent into the ionization chamber.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    StandardSourceTemperatures

    For each member of Standards, the temperature that the source block is set to in order to discourage condensation and decrease solvent clustering in the reduced vacuum region of the source. The source block is the metallic chamber with reduced pressure separating the sprayer (at atmospheric pressure) and the inside of the mass spectrometer (at 10^-7 Torr of pressure). It consists of the sampling cone, the isolation valve, and the ion block. The source temperature setting affects sensitivity.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    StandardESICapillaryVoltages

    For each member of Standards, the applied voltage differential between the injector and the inlet plate for the mass spectrometry in order to ionize analyte molecules.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    StandardSamplingConeVoltages

    For each member of Standards, the voltage offset between the 1st and 2nd stage of the stepwave ion guide which leads ions coming from the sample cone (the metal shield covering the front of the source block), towards the quadrupole mass analyzer.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    StandardScanTimes

    For each member of Standards, the duration of time allowed to pass between each spectral acquisition.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    StandardMassDetectionGains

    For each member of Standards, indicates the arbitrary-scaled signal amplification of the mass spectrometry measurement.
    Format: Multiple
    Class: Real
    Programmatic Pattern: RangeP[0.01, 1000., 0.01]

    StandardAbsorbanceSelection

    For each member of Standards, all the wavelengths of light absorbed in the detector's flow cell for a PhotoDiodeArray (PDA) detector.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterP[0 Centimeter]..}

    StandardMinAbsorbance Wavelengths

    For each member of Standards, the wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    StandardMaxAbsorbance Wavelengths

    For each member of Standards, the maximum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    StandardWavelengthResolutions

    For each member of Standards, the increment in wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    StandardUVFilters

    For each member of Standards, indicates whether or not to block UV wavelengths (less than 210 nm) from being transmitted through the sample for Absorbance detectors (PDA).
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    StandardAbsorbanceSampling Rates

    For each member of Standards, indicates the frequency of measurement for PDA detectors.
    Format: Multiple
    Class: Real
    Unit: 1 reciprocal second
    Programmatic Pattern: 1 Hold[GreaterP[0 ------]] Second

    StandardsStorageConditions

    For each member of Standards, the storage conditions under which the standard samples should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Blanking

    Blanks

    Buffer only samples injected to asses background signal from the buffers and column in the absence of injected analyte.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    BlankSampleVolumes

    For each member of Blanks, the volume taken from the blank and injected onto the column.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Micro Liter]

    BlankGradientMethods

    For each member of Blanks, the method used to describe the gradient used for purification.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Method]

    BlankCO2Gradient

    For each member of Blanks, the percentage of supercritical CO2 in the composition over time, in the form: {Time, % CO2} or a single % CO2 for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BlankGradientA

    For each member of Blanks, the percentage of CosolventA in the composition over time, in the form: {Time, % Cosolvent A} or a single % Cosolvent A for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BlankGradientB

    For each member of Blanks, the percentage of CosolventB in the composition over time, in the form: {Time, % Cosolvent B} or a single % Cosolvent B for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BlankGradientC

    For each member of Blanks, the percentage of CosolventC in the composition over time, in the form: {Time, % Cosolvent C} or a single % Cosolvent C for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BlankGradientD

    For each member of Blanks, the percentage of CosolventD in the composition over time, in the form: {Time, % Cosolvent D} or a single % Cosolvent D for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    BlankBackPressures

    For each member of Blanks, the applied pressure between the atmosphere and the PhotoDiodeArray detector exit, in the form: {Time, BackPressure} or a single BackPressure for the entire run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], GreaterP[0 PSI]}...} | GreaterP[0 PSI]

    BlankFlowRates

    For each member of Blanks, the total rate of mobile phase pumped through the instrument.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    BlankColumns

    For each member of Blanks, the column used from the column selector for the separation and measurement.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    BlankColumnTemperatures

    For each member of Blanks, the nominal temperature of the column compartment during a run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    BlankIonModes

    For each member of Blanks, indicates if positively or negatively charged ions are analyzed each column prime run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IonModeP

    BlankMakeupFlowRates

    For each member of Blanks, the total rate of MakeupSolvent pumped through the instrument for each column prime.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    BlankMassSelection

    For each member of Blanks, all of the mass-to-charge ratio (m/z) measured via mass spectrometry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: 0 Gram Hold[{GreaterP[------]..}] Mole

    BlankMinMasses

    For each member of Blanks, the lowest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterEqualP[------]] Mole

    BlankMaxMasses

    For each member of Blanks, the highest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterP[------]] Mole

    BlankProbeTemperatures

    For each member of Blanks, the temperature of the needle that sprays column effluent into the ionization chamber.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    BlankSourceTemperatures

    For each member of Blanks, the temperature that the source block is set to in order to discourage condensation and decrease solvent clustering in the reduced vacuum region of the source. The source block is the metallic chamber with reduced pressure separating the sprayer (at atmospheric pressure) and the inside of the mass spectrometer (at 10^-7 Torr of pressure). It consists of the sampling cone, the isolation valve, and the ion block. The source temperature setting affects sensitivity.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    BlankESICapillaryVoltages

    For each member of Blanks, the applied voltage differential between the injector and the inlet plate for the mass spectrometry in order to ionize analyte molecules.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    BlankSamplingConeVoltages

    For each member of Blanks, the voltage offset between the 1st and 2nd stage of the stepwave ion guide which leads ions coming from the sample cone (the metal shield covering the front of the source block), towards the quadrupole mass analyzer.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    BlankScanTimes

    For each member of Blanks, the duration of time allowed to pass between each spectral acquisition.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    BlankMassDetectionGains

    For each member of Blanks, indicates the arbitrary-scaled signal amplification of the mass spectrometry measurement.
    Format: Multiple
    Class: Real
    Programmatic Pattern: RangeP[0.01, 1000., 0.01]

    BlankAbsorbanceSelection

    For each member of Blanks, all the wavelengths of light absorbed in the detector's flow cell for a PhotoDiodeArray (PDA) detector.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterP[0 Centimeter]..}

    BlankMinAbsorbanceWavelengths

    For each member of Blanks, the wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    BlankMaxAbsorbanceWavelengths

    For each member of Blanks, the maximum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    BlankWavelengthResolutions

    For each member of Blanks, the increment in wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    BlankUVFilters

    For each member of Blanks, indicates whether or not to block UV wavelengths (less than 210 nm) from being transmitted through the sample for Absorbance detectors (UVVis or PDA).
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    BlankAbsorbanceSamplingRates

    For each member of Blanks, indicates the frequency of measurement for UVVis or PDA detectors.
    Format: Multiple
    Class: Real
    Unit: 1 reciprocal second
    Programmatic Pattern: 1 Hold[GreaterP[0 ------]] Second

    BlanksStorageConditions

    For each member of Blanks, the storage conditions under which the blank samples should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Column Flush

    ColumnFlushGradientMethods

    For each member of ColumnSelection, the composition of the CO2 and cosolvents within the flow, defined for specific time points during the washing of the columns (column flush).
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Method]

    ColumnFlushCO2Gradient

    For each member of ColumnSelection, the percentage of supercritical CO2 in the composition over time, in the form: {Time, % CO2} or a single % CO2 for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnFlushGradientA

    For each member of ColumnSelection, the percentage of CosolventA in the composition over time, in the form: {Time, % Cosolvent A} or a single % Cosolvent A for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnFlushGradientB

    For each member of ColumnSelection, the percentage of CosolventB in the composition over time, in the form: {Time, % Cosolvent B} or a single % Cosolvent B for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnFlushGradientC

    For each member of ColumnSelection, the percentage of CosolventC in the composition over time, in the form: {Time, % Cosolvent C} or a single % Cosolvent C for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnFlushGradientD

    For each member of ColumnSelection, the percentage of CosolventD in the composition over time, in the form: {Time, % Cosolvent D} or a single % Cosolvent D for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]

    ColumnFlushBackPressures

    For each member of ColumnSelection, the applied pressure between the atmosphere and the PhotoDiodeArray detector exit, in the form: {Time, BackPressure} or a single BackPressure for each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {{GreaterEqualP[0 Minute], GreaterP[0 PSI]}...} | GreaterP[0 PSI]

    ColumnFlushFlowRates

    For each member of ColumnSelection, the total rate of mobile phase pumped through the instrument for each column flush.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    ColumnFlushTemperatures

    For each member of ColumnSelection, the nominal temperature of the column compartment for each column flush run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Kelvin]

    ColumnFlushIonModes

    For each member of ColumnSelection, indicates if positively or negatively charged ions are analyzed each column flush run.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IonModeP

    ColumnFlushMakeupFlowRates

    For each member of ColumnSelection, the total rate of MakeupSolvent pumped through the instrument for each column flush.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milliliter 0 Milli Liter Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]] Minute Minute

    ColumnFlushMassSelections

    For each member of ColumnSelection, the all of the mass-to-charge ratio (m/z) measured via mass spectrometry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: 0 Gram Hold[{GreaterP[------]..}] Mole

    ColumnFlushMinMasses

    For each member of ColumnSelection, the lowest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterEqualP[------]] Mole

    ColumnFlushMaxMasses

    For each member of ColumnSelection, the highest measured mass-to-charge ratio (m/z) value.
    Format: Multiple
    Class: Real
    Unit: 1 gram per mole
    Programmatic Pattern: 0 Gram Hold[GreaterP[------]] Mole

    ColumnFlushProbeTemperatures

    For each member of ColumnSelection, the temperature of the needle that sprays column effluent into the ionization chamber for each ColumnFlush run.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    ColumnFlushSourceTemperatures

    For each member of ColumnSelection, the temperature that the source block is set to in order to discourage condensation and decrease solvent clustering in the reduced vacuum region of the source. The source block is the metallic chamber with reduced pressure separating the sprayer (at atmospheric pressure) and the inside of the mass spectrometer (at 10^-7 Torr of pressure). It consists of the sampling cone, the isolation valve, and the ion block. The source temperature setting affects sensitivity.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Celsius]

    ColumnFlushESICapillary Voltages

    For each member of ColumnSelection, the applied voltage differential between the injector and the inlet plate for the mass spectrometry in order to ionize analyte molecules.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    ColumnFlushSamplingCone Voltages

    For each member of ColumnSelection, the voltage offset between the 1st and 2nd stage of the stepwave ion guide which leads ions coming from the sample cone (the metal shield covering the front of the source block), towards the quadrupole mass analyzer.
    Format: Multiple
    Class: Real
    Unit: 1 volt
    Programmatic Pattern: GreaterP[0 Volt]

    ColumnFlushScanTimes

    For each member of ColumnSelection, the duration of time allowed to pass between each spectral acquisition.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    ColumnFlushMassDetectionGains

    For each member of ColumnSelection, indicates the arbitrary-scaled signal amplification of the mass spectrometry measurement.
    Format: Multiple
    Class: Real
    Programmatic Pattern: RangeP[0.01, 1000., 0.01]

    ColumnFlushAbsorbance Selection

    For each member of ColumnSelection, all the wavelengths of light absorbed in the detector's flow cell for a PhotoDiodeArray (PDA) detector.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {GreaterP[0 Centimeter]..}

    ColumnFlushMinAbsorbance Wavelengths

    For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnFlushMaxAbsorbance Wavelengths

    For each member of ColumnSelection, the maximum wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnFlushWavelength Resolutions

    For each member of ColumnSelection, the increment in wavelength of light absorbed in the detector's flow cell for a PDA detector.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Centimeter]

    ColumnFlushUVFilters

    For each member of ColumnSelection, indicates whether or not to block UV wavelengths (less than 210 nm) from being transmitted through the sample for PDA detector.
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    ColumnFlushAbsorbanceSampling Rates

    For each member of ColumnSelection, indicates the frequency of measurement for UVVis or PDA detectors.
    Format: Multiple
    Class: Real
    Unit: 1 reciprocal second
    Programmatic Pattern: 1 Hold[GreaterP[0 ------]] Second
Last modified on Mon 26 Sep 2022 15:43:51