ECL`

AnalyzeLadder

fits a standard curve function to molecular size (e.g. oligomer length) from peak position.

fits the standard curve to peak positions taken from the most recent peaks analysis linked to the given data object. Runs AnalyzePeaks if no linked analysis object is found.

Details

Input

Output

General Options

Method Options

Examples

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Basic Examples (5)

Fit standard curve to chromatogram peaks:

Fit standard curve to PAGE lane intensity peaks:

Fit standard curve to peaks analysis:

Plot a standard fit:

Plot standard sizes associated with their peaks:

Additional Examples (4)

Analyze a list of data objects:

Analyze a list of peaks objects:

Fit standard curve to CESDS peaks:

Fit standard curve to Western MassSpectrum peaks:

Options (19)

Align (3)

Align ladder starting with minimum peak position, and reverse ladder:

Associate right:

Comparing alignment at maximum and minimum:

ExpectedSizes (3)

Specify a smaller standard set:

Specify standard sizes:

Specify standard sizes Model[Sample, StockSolution, Standard]:

FitType (2)

Fit cubic ExpectedSize:

Fit linear ExpectedSize:

Function (2)

Fit ExpectedPosition function:

Fit ExpectedPosition function and return inverse function ExpectedSize:

Name (1)

Assign a name to the analyze ladder object:

Output (2)

Return the entire packet:

Return the preview:

PlotType (1)

Display peaks data on the preview plot:

ReferenceField (1)

Use the peaks from the Absorbance field of the data object:

Reverse (2)

Comparing Reverse options:

Reverse:

Template (2)

Explicitly specify FitType option, and pull remaining options from previous ladder analysis:

Use options from previous ladder analysis:

Messages (6)

AnalyzePeaksFailure (1)

Calling AnalyzeLadder on a ReferenceField that does not interact well with AnalyzePeaks will result in an error:

DefaultSizes (1)

Calling AnalyzeLadder on a data object that does not have a properly set Composition field of the linked Samples object results in a warning that a default ladder size set is being used:

NoPickedPeaks (1)

Given peaks object has no picked peaks:

PeaksFieldEmpty (2)

Conducts Analyze Peaks, but Fail because only 1 peak picked.

Input CESDS object has not had its RelativeMigrationData field peak-picked yet:

ReferenceFieldNotResolved (1)

If the given reference field is not a valid peaks field of the data object, Automatic will be used instead:

Last modified on Thu 11 Sep 2025 14:58:44