ExperimentCapillaryGelElectrophoresisSDS
ExperimentCapillaryGelElectrophoresisSDS[Samples]⟹Protocol
generates a Protocol object for running capillary Gel Electrophoresis-SDS (CESDS) on protein Samples. CESDS is an analytical method used to separate proteins by their molecular weight.
Capillary Electrophoresis-Sodium Dodecyl Sulfate (CESDS) is an analytical technique to separate denatured proteins. Samples are mixed with an internal standard and electrokinetically injected into a capillary filled with a separation matrix. Voltage is then applied across the capillary and analytes migrate in the matrix by their electrophoretic mobility. The capillary is imaged over time (UV absorbance) and the migration of different proteins, relative to the internal standard, is tracked. CESDS is useful for purity and heterogeneity studies, and while optimized for the analysis of Immunoglobulin G, any protein can be analyzed when paired with molecular weight markers.
Experimental Principles
Figure 1.1: Procedural overview of a Capillary Gel Electrophoresis SDS (CESDS) experiment. Step 1: Samples are mixed with an internal reference (10KDa marker), a sample buffer containing SDS buffer to a final concentration if 0.5% or more, and, optionally, a reducing (e.g., Tris(2-carboxyethyl)phosphine, β-mercaptoethanol, or Dithiothreitol) and/or an alkylating agent (e.g., Iodoacetamide).Step 2: Subsequently, samples are incubated at a high temperature to denature proteins and centrifuged (optional). Step 3: The capillary is prepared by subsequent washes with a conditioning acid, conditioning base and water (every 12 injections), and a separation matrix is loaded into the capillary. Step 4: The capillary is placed into a sample and a voltage is applied to electrokinetically inject proteins (negatively-charged due to SDS in sample buffer) into the separation matrix. Step 5: The capillary is docked in the running buffer and a voltage is applied to separate proteins in the sample. Proteins are detected at the end of the capillary by measurement of transmitted light as a result of excitation of peptide bonds by UV light at 220 nanometers. The capillary is then washed and dried and contents disposed into the top running buffer compartment onboard the experiment cartridge. Subsequently, the tip is washed and the capillary is prepared to load the next sample (Step 3). Step 6: Data is recorded in real time as proteins cross the detection window (absorbance as a factor of migration time).
Instrumentation
Maurice
Figure 2.1.1: Instrument diagram for the ProteinSimple Maurice system: The instrument has several positions for reagents, the assay plate, and the experiment Cartridge that holds the capillary on which electrophoretic separation takes place. Reagents are maintained at room temperature while samples can be kept at a predetermined temperature (4°C, 10°C, 15°C, or ambient). The reagents and assay plate are positioned on a mobile platform that facilitates capillary preparation and sampling while the cartridge remains stationary. The capillary is washed and conditioned by applying reagent in high pressure. Conditioning with an acid, a base, and water takes place every twelve injections, while the capillary is washed, dried, and fresh separation matrix is loaded every injection. The experiment cartridge contains a single uncoated silica capillary that is 17 centimeters long and 50 microns in diameter. While samples are separating, the cartridge is docked in a running buffer and proteins are detected through a detection window at the top of the capillary by measuring transmitted light from a concentrated UV light beam at 220 nanometers. All samples, standards, ladders, and blanks are mixed with an internal standard that allows data to be comparable. Electropherograms need to be normalized based on the internal standard to generate comparable data (relative migration time).
Experiment Options
General
Instrument
The capillary electrophoresis instrument that will be used by the protocol. The instrument accepts the cartridge loaded with running buffer and sequentially loads samples and resolves proteins by their size by electrophoresis through a separation matrix.
Pattern Description: An object of type or subtype Model[Instrument, ProteinCapillaryElectrophoresis] or Object[Instrument, ProteinCapillaryElectrophoresis]
Programmatic Pattern: ObjectP[{Model[Instrument, ProteinCapillaryElectrophoresis], Object[Instrument, ProteinCapillaryElectrophoresis]}]
Cartridge
The capillary electrophoresis cartridge loaded on the instrument for Capillary gel Electrophoresis-SDS (CESDS) experiments. The cartridge holds a single capillary and the anode's running buffer. CESDS cartridges can run 100 injections in up to 25 batches.
Pattern Description: An object of type or subtype Model[Container, ProteinCapillaryElectrophoresisCartridge] or Object[Container, ProteinCapillaryElectrophoresisCartridge] or a prepared sample.
Programmatic Pattern: ObjectP[{Model[Container, ProteinCapillaryElectrophoresisCartridge], Object[Container, ProteinCapillaryElectrophoresisCartridge]}] | _String
PurgeCartridge
Indicates if a Cartridge purge be performed before running the experiment unless there is a single batch left on the cartridge (uses an extra consumable and would count as an extra batch towards the 25 batch limit). When a cartridge has not been used for over 3 months a purge is highly recommended.
SampleTemperature
InjectionTable
The order of sample, Ladder, Standard, and Blank sample loading into the Instrument during measurement.
Default Calculation: Determined to the order of input samples articulated. Ladder, Standard and Blank samples are inserted based on the determination of LadderFrequency, StandardFrequency and BlankFrequency. For example, StandardFrequency -> FirstAndLast and BlankFrequency -> Null result in Standard samples injected first, then samples, and then the Standard sample set again at the end. A ladder sample will be loaded for every unique set of conditions (MasterMix and separation conditions) specified for SamplesIn, Standards, and Blanks, in replicates as determined by LadderFrequency.
Programmatic Pattern: {{Sample | Ladder | Standard | Blank, ObjectP[{Model[Sample], Object[Sample]}] | _String, RangeP[0*Microliter, 200*Microliter] | Automatic}..} | Automatic
NumberOfReplicates
The number of times each sample will be injected. For example, when NumberOfReplicates is set to 2, each sample will be run twice consecutively. By default, this option means technical replicates that are injected from the same position on the assay plate. Unless different aliquot containers are used for the replicates with ConsolidateAliquots->False, the replicates will be injected from different aliquots of the sample.
Instrument Preparation
ConditioningAcid
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
ConditioningBase
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
ConditioningWashSolution
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
SeparationMatrix
The sieving matrix loaded onto the capillary between samples for separation. The mash-like matrix slows the migration of proteins based on their size.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
SystemWashSolution
The solution used to wash the capillary after conditioning and, separately, rinse the tip before every injection.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
ConditioningAcidStorageCondition
The non-default storage condition for ConditioningAcid of this experiment after the protocol is completed. If left unset, samples will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
ConditioningBaseStorageCondition
The non-default storage condition for ConditioningBase of this experiment after the protocol is completed. If left unset, samples will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
SeparationMatrixStorageCondition
The non-default storage condition for SeparationMatrix of this experiment after the protocol is completed. If left unset, samples will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
SystemWashSolutionStorageCondition
The non-default storage condition for SystemWashSolution of this experiment after the protocol is completed. If left unset, samples will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
RunningBufferStorageCondition
The non-default storage condition for Bottom RunningBuffer of this experiment after the protocol is completed. If left unset, Bottom RunningBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Denaturation
Denature
DenaturingTemperature
Default Calculation: When Denature is True and DenaturingTemperature is set to Automatic, it will be set to 70 celsius. Alternatively, if Denature is False, it will be set to Null.
Pattern Description: Greater than or equal to 25 degrees Celsius and less than or equal to 100 degrees Celsius or Null.
DenaturingTime
Default Calculation: When Denature is True and DenaturingTime is set to Automatic, it will be set to 10 minutes. Alternatively, if Denature is False, it will be set to Null.
CoolingTemperature
Default Calculation: When Denature is True and CoolingTemperature is set to Automatic, it will be set to 4 celsius. Alternatively, if Denature is False, it will be set to Null.
Pattern Description: Greater than or equal to 0 degrees Celsius and less than or equal to 25 degrees Celsius or Null.
CoolingTime
Default Calculation: When Denature is True and CoolingTime is set to Automatic, it will be set to 5 minutes. Alternatively, if Denature is False, it will be set to Null.
PelletSedimentation
Indicates if centrifugation should be applied to the sample to remove precipitates after denaturation.
SedimentationCentrifugationSpeed
Default Calculation: When PelletSedimentation is True and SedimentationCentrifugationSpeed is set to Automatic, it will be calculated from force based on the rotor's radius.
Pattern Description: Greater than or equal to 0 revolutions per minute and less than or equal to 4150 revolutions per minute or Null.
SedimentationCentrifugationForce
Default Calculation: When PelletSedimentation is True and SedimentationCentrifugationForce is set to Automatic, it will be calculated from Speed based on the rotor's radius, if speed is set to Automatic/Null, will default to 1000xg.
Pattern Description: Greater than or equal to 0 standard accelerations due to gravity on the surface of the earth and less than or equal to 3755 standard accelerations due to gravity on the surface of the earth or Null.
Programmatic Pattern: (RangeP[0*GravitationalAcceleration, 3755*GravitationalAcceleration] | Automatic) | Null
SedimentationCentrifugationTime
SedimentationCentrifugationTemperature
Pattern Description: Greater than or equal to 0 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
Sample Preparation
SampleVolume
Indicates the volume drawn from the sample to the assay tube. Each tube contains a Sample, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Default Calculation: When SampleVolume is set to Automatic, the volume is calculated for the composition of the sample to reach 1 mg / ml by 1 Milligram/Milliliter * TotalVolume / Sample [Concentration]. If an injection table is available, volumes will be taken from it.
TotalVolume
Indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a Sample, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Pattern Description: Greater than or equal to 50 microliters and less than or equal to 200 microliters.
PremadeMasterMix
Indicates if samples should be mixed with PremadeMasterMix that includes an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When PremadeMasterMix is set to Automatic, it will resolve to True if any of its downstream options is specified.
PremadeMasterMixReagent
The premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
PremadeMasterMixDiluent
Default Calculation: When PremadeMasterMix is set to True and PremadeMasterMixDiluent is set to Automatic, Model[Sample,"Milli-Q water"] will be set as diluent.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
PremadeMasterMixReagentDilutionFactor
Default Calculation: When PremadeMasterMix is set to True and PremadeMasterMixReagentDilutionFactor is set to Automatic, it will be set as the ratio of the total volume to premade mastermix volume.
PremadeMasterMixVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of TotalVolume by PremadeMasterMixReagentDilutionFactor. If PremadeMasterMix is set to False, PremadeMasterMixVolume is Null.
PremadeMasterMixStorageCondition
The non-default storage condition for PremadeMasterMix of this experiment after the protocol is completed. If left unset, Diluent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
InternalReference
The solution containing the reference analyte by which Relative Migration Time is normalized. By default a 10 KDa marker is used. It is highly recommended to include an internal standard in Sample Preparation.
Default Calculation: When PremadeMasterMix is set to False and InternalReference is set to Automatic, InternalReference will be set as Model[Sample, StockSolution, "Resuspended CESDS Internal Standard 25X"]. If PremadeMasterMix is set to True, InternalReference is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
InternalReferenceDilutionFactor
Marks how concentrated the internal standard is. For example, Model[Sample, StockSolution"Resuspended CESDS Internal Standard 25X"] is concentrated 25X, hence 2 µL should be added in Sample Preparation when final volume is set to 60 microliters.
Default Calculation: When PremadeMasterMix is set to False and InternalReferenceDilutionFactor is set to Automatic, InternalReferenceDilutionFactor will be set to 25. If PremadeMasterMix is set to True, InternalReferenceDilutionFactor is Null.
InternalReferenceVolume
Default Calculation: When InternalReferenceVolume is set to Automatic and PremadeMasterMix is set to False, the volume is calculated by the division of TotalVolume by InternalReferenceDilutionFactor. When PremadeMasterMix is set to True, InternalReferenceVolume is Null.
ConcentratedSDSBuffer
The SDS Buffer used to dilute the sample. The final concentration of SDS in this assay must be equal to or greater than 0.5%.
Default Calculation: When PremadeMasterMix is set to False and ConcentratedSDSBuffer is set to Automatic, ConcentratedSDSBuffer will be set as Model[Sample,"1% SDS in 100mM Tris, pH 9.5"]. If PremadeMasterMix is set to True, ConcentratedSDSBuffer is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
ConcentratedSDSBufferDilutionFactor
Marks how concentrated the SDS buffer is. For example, a ConcentratedSDSBuffer that contains 1% SDS is concentrated 2X and will constitute half the TotalVolume.
Default Calculation: When PremadeMasterMix is set to False and ConcentratedSDSBufferDilutionFactor is set to Automatic, ConcentratedSDSBufferDilutionFactor will be set to 2. If PremadeMasterMix is set to True, ConcentratedSDSBufferDilutionFactor is Null.
Diluent
Default Calculation: When PremadeMasterMix is set to False and Diluent is set to Automatic, Diluent will be set to Model[Sample,"Milli-Q water"]. If PremadeMasterMix is set to True, Diluent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
ConcentratedSDSBufferVolume
Default Calculation: When volume is set to Automatic and PremadeMasterMix is set to False, the volume is calculated by the division of TotalVolume by ConcentratedSDSBufferDilutionFactor. When PremadeMasterMix is set to True, ConcentratedSDSBufferVolume is Null.
SDSBuffer
The SDS Buffer used to dilute the sample. The final concentration of SDS in this assay must be equal or greater than 0.5%. If ConcentratedSDSBuffer is set to Null, SDSBuffer will be used to dilute the sample.
Default Calculation: When PremadeMasterMix is set to False ,ConcentratedSDSBuffer is set to Null, and SDSBuffer is set to Automatic, SDSBuffer will be set as Model[Sample,"1% SDS in 100mM Tris, pH 9.5"]. If PremadeMasterMix or ConcentratedSDSBuffer are set, SDSBuffer is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
SDSBufferVolume
Default Calculation: When SDSBufferVolume is set to Automatic and SDSBuffer is not Null, the volume is calculated by the difference between the TotalVolume and the volume in the tube that includes the Sample, InternalReference, ReducingAgent, and AlkylatingAgent.
Reduction
Default Calculation: When automatic, Reduction will set to True if any of the Reduction options are not Null.
ReducingAgent
The reducing agent used to reduce disulfide bridges in proteins to be added to the sample. for example, β-mercaptoethanol or Dithiothreitol.
Default Calculation: When PremadeMasterMix is set to False and ReducingAgent is set to Automatic, ReducingAgent will be set to Model[Sample,"2-mercaptoethanol"]. If PremadeMasterMix is set to True, ReducingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
ReducingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, PremadeMasterMix is set to False, and no ReducingAgentVolume value is given, concentration is set to 650 millimolar if ReducingAgent is BME. If volume is given concentration will be calculated. If PremadeMasterMix is set to True, ReducingAgentTargetConcentration is Null
Pattern Description: Greater than 0 molar or greater than or equal to 0 VolumePercent and less than or equal to 100 VolumePercent or Null.
Programmatic Pattern: ((GreaterP[0*Molar] | RangeP[0*VolumePercent, 100*VolumePercent]) | Automatic) | Null
ReducingAgentVolume
Default Calculation: When ReducingAgentVolume is set to Automatic and PremadeMasterMix is set to False, calculate the volume required to reach ReducingAgentTargetConcentration, if set. When ReducingAgentVolume is set to Automatic and ReducingAgentTargetConcentration is set to Null, ReducingAgentVolume is set to Null. When PremadeMasterMix is set to True, ReducingAgentVolume is Null.
Alkylation
Indicates if Alkylation should be applied to the sample. Alkylation of free cysteines is useful in mitigating unexpected protein fragmentation and reproducibility issues.
Default Calculation: When automatic, Alkylation will set to True if any of the Reduction options are not Null.
AlkylatingAgent
Default Calculation: When PremadeMasterMix is set to False and AlkylatingAgent is set to Automatic, AlkylatingAgent will be set to Model[Sample,"250 mM Iodoacetamide"]. If PremadeMasterMix is set to True, AlkylatingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AlkylatingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, PremadeMasterMix is set to False, and no AlkylatingAgentVolume value is given, concentration is set to 11.5 millimolar. If volume is given concentration will be calculated. If PremadeMasterMix is set to True, AlkylatingAgentTargetConcentration is Null.
AlkylatingAgentVolume
Default Calculation: When AlkylatingAgentVolume is set to Automatic and PremadeMasterMix is set to False, calculate the volume required to reach AlkylatingAgentTargetConcentration, if set. When AlkylatingAgentVolume is set to Automatic and AlkylatingAgentTargetConcentration is set to Null, AlkylatingAgentVolume is set to Null. If PremadeMasterMix is set to True, AlkylatingAgentVolume is Null.
SedimentationSupernatantVolume
The volume of supernatant to transfer to the assay container from the sample tubes after denaturation and centrifugation. A minimum of 50 microliters are required for the analysis to proceed.
Default Calculation: When SedimentationSupernatantVolume is set to Automatic, 90% of the TotalVolume is drawn to avoid disturbing the pellet.
Storage Conditions
InternalReferenceStorageCondition
The non-default storage condition for InternalReference of this experiment after samples are transferred to assay tubes. If left unset, InternalReference will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
ConcentratedSDSBufferStorageCondition
The non-default storage condition for ConcentratedSDSBuffer of this experiment after samples are transferred to assay tubes. If left unset, ConcentratedSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
DiluentStorageCondition
The non-default storage condition for Diluent of this experiment after the protocol is completed. If left unset, Diluent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
SDSBufferStorageCondition
The non-default storage condition for SDSBuffer of this experiment after samples are transferred to assay tubes. If left unset, SDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
ReducingAgentStorageCondition
The non-default storage condition for ReducingAgent of this experiment after samples are transferred to assay tubes. If left unset, ReducingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
AlkylatingAgentStorageCondition
The non-default storage condition for AlkylatingAgent of this experiment after samples are transferred to assay tubes. If left unset, AlkylatingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
CartridgeStorageCondition
The non-default storage condition for the Cartridge after the protocol is completed. If left unset, Cartridge will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Separation conditions
RunningBuffer
The buffer in which the capillary docks for separation. This buffer must be compatible with the running buffer loaded on the CESDS cartridge (see: OnboardRunningBuffer field of the selected Cartridge).
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample.
InjectionVoltageDurationProfile
Series of voltages and durations to apply onto the capillary for injection. Supports up to 20 steps.
SeparationVoltageDurationProfile
Series of voltages and durations to apply onto the capillary for separation. Supports up to 20 steps.
Default Calculation: When SeparationVoltageDurationProfile is set to Automatic, separation time will be set according to whether or not samples are reduced or not, and which Standard is used. If standard is IgG and samples are not reduced, set time to 25 minutes, otherwise set to 35 minutes. For all conditions, voltage is set to 5750 Volts.
Detection
UVDetectionWavelength
The wavelength used for signal detection. The hardware is currently only capable of detection at 220 nm.
Ladders
IncludeLadders
Indicates if mixtures of known analytes should be included in this experiment. A ladder contains a mixture of analytes of known size and Relative Migration Times and can be used to interpolate the molecular weight of unknown analytes. The ladder will be injected for every unique Separation set of conditions.
Default Calculation: If any ladder-related options are set, then this is set True; otherwise, false.
Ladders
Indicates the premade mixture of analytes of known molecular weight (MW) to include as reference for MW interpolation in this experiment.
Default Calculation: If IncludeLadder is True, Ladders is resolved to Model[Sample, "Unstained Protein Standard"].
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderStorageCondition
The non-default storage condition for Ladders of this experiment after the protocol is completed. If left unset, LadderStorageCondition will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderAnalytes
Default Calculation: If composition of Ladder is known, will populate LadderAnalytes accordingly. Otherwise, Null.
Pattern Description: List of one or more an object of type or subtype Model[Molecule] entries or Null.
LadderAnalyteMolecularWeights
Default Calculation: If composition of Ladder is known, will populate LadderAnalytesMolecularWeights accordingly. Otherwise, Null.
LadderAnalyteLabels
Default Calculation: Will populate LadderAnalyteLabels according to LadderAnalytes, if set. Otherwise, Will populate LadderAnalyteLabels according to LadderAnalyteMolecularWeights.
LadderFrequency
Indicates how many injections per permutation of ladder and unique set of separation conditions should be included in this experiment.
Default Calculation: If LadderFrequency is set to Automatic and Ladders is True, LadderFrequency will be set to FirstAndLast.
Ladder Preparation
LadderTotalVolume
Indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a ladder, an InternalReference, and an SDSBuffer.
Default Calculation: If Automatic and Ladders is True, will populate LadderTotalVolume according to TotalVolume.
Pattern Description: Greater than or equal to 50 microliters and less than or equal to 200 microliters or Null.
LadderDilutionFactor
LadderVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of LadderTotalVolume by LadderDilutionFactor. If LadderDilutionFactor is Null, 40 Microliters will be used. If InjectionTable is informed, specified volumes will be used.
LadderPremadeMasterMix
Indicates if Ladders should be mixed with LadderPremadeMasterMix that includes an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When LadderPremadeMasterMix is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderPremadeMasterMixReagent
The premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When LadderPremadeMasterMixReagent is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderPremadeMasterMixDiluent
Default Calculation: When LadderPremadeMasterMix is set to True and LadderPremadeMasterMixDiluent is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderPremadeMasterMixReagentDilutionFactor
Default Calculation: When LadderPremadeMasterMix is set to True and LadderPremadeMasterMixReagentDilutionFactor is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderPremadeMasterMixVolume
Default Calculation: When volume is set to Automatic, and LadderPremadeMasterMix is set to True, it will resolve according to each unique set of options applied to SamplesIn.
LadderPremadeMasterMixStorageCondition
The non-default storage condition for LadderPremadeMasterMix of this experiment after the protocol is completed. If left unset, LadderPremadeMasterMix will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderInternalReference
The solution containing the reference analyte by which Relative Migration Time is normalized. By default a 10 KDa marker is used. It is highly recommended to include an internal standard in Sample Preparation.
Default Calculation: When LadderPremadeMasterMix is set to False and InternalReference is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderInternalReferenceDilutionFactor
Marks how concentrated the internal standard is. For example, Model[Sample,"CESDS Internal Reference 25X"] is concentrated 25X, hence 2 µL should be added in Sample Preparation when final volume is set to 50 µL.
Default Calculation: When LadderPremadeMasterMix is set to False and LadderInternalReferenceDilutionFactor is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderInternalReferenceVolume
Default Calculation: When volume is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderConcentratedSDSBuffer
The SDS Buffer used to dilute standards. The final concentration of SDS in this assay must be equal to or greater than 0.5%.
Default Calculation: When LadderPremadeMasterMix is set to False and LadderConcentratedSDSBuffer is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderConcentratedSDSBufferDilutionFactor
Marks how concentrated the LadderConcentratedSDSBuffer is. For example, a LadderConcentratedSDSBuffer that contains 1% SDS is concentrated 2X and will constitute half the LadderTotalVolume.
Default Calculation: When LadderPremadeMasterMix is set to False and LadderConcentratedSDSBufferDilutionFactor is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderDiluent
Default Calculation: When LadderPremadeMasterMix is set to False and Diluent is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderConcentratedSDSBufferVolume
Default Calculation: When volume is set to Automatic and LadderPremadeMasterMix is set to False, it will resolve according to each unique set of options applied to SamplesIn.
LadderSDSBuffer
The SDS Buffer used to dilute the sample. The final concentration of SDS in this assay must be equal or greater than 0.5%.
Default Calculation: When SDS Buffer is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderSDSBufferVolume
Default Calculation: When LadderSDSBufferVolume is set to Automatic and LadderSDSBuffer is not Null, it will resolve according to each unique set of options applied to SamplesIn.
LadderReduction
Default Calculation: When automatic, LadderReduction will set to True if any of the Reduction options are not Null.
LadderReducingAgent
Default Calculation: When LadderPremadeMasterMix is set to False and LadderReducingAgent is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderReducingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: Greater than 0 molar or greater than or equal to 0 VolumePercent and less than or equal to 100 VolumePercent or Null.
Programmatic Pattern: ((GreaterP[0*Molar] | RangeP[0*VolumePercent, 100*VolumePercent]) | Automatic) | Null
LadderReducingAgentVolume
Default Calculation: When LadderReducingAgentVolume is set to Automatic and LadderPremadeMasterMix is set to False, it will resolve according to each unique set of options applied to SamplesIn.
LadderAlkylation
Indicates if Alkylation should be applied to the Ladder. Alkylation of free cysteines is useful in mitigating unexpected protein fragmentation and reproducibility issues.
Default Calculation: When automatic, LadderAlkylation will resolve according to each unique set of options applied to SamplesIn.
LadderAlkylatingAgent
Default Calculation: When LadderPremadeMasterMix is set to False and LadderAlkylatingAgent is set to Automatic it will resolve according to each unique set of options applied to SamplesIn.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
LadderAlkylatingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic it will resolve according to each unique set of options applied to SamplesIn.
LadderAlkylatingAgentVolume
Default Calculation: When LadderAlkylatingAgentVolume is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderSedimentationSupernatantVolume
The volume of supernatant to transfer to the assay container from the Ladder tubes after denaturation and centrifugation. A minimum of 50 µL are required for the analysis to proceed.
Default Calculation: When SedimentationSupernatantVolume is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
LadderInternalReferenceStorageCondition
The non-default storage condition for LadderInternalReference of this experiment after the protocol is completed. If left unset, LadderInternalReferences will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderConcentratedSDSBufferStorageCondition
The non-default storage condition for LadderConcentratedSDSBuffer of this experiment after the protocol is completed. If left unset, LadderConcentratedSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderDiluentStorageCondition
The non-default storage condition for LadderDiluent of this experiment after the protocol is completed. If left unset, LadderDiluent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderSDSBufferStorageCondition
The non-default storage condition for LadderSDSBuffer of this experiment after the protocol is completed. If left unset, LadderSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderReducingAgentStorageCondition
The non-default storage condition for LadderReducingAgent of this experiment after the protocol is completed. If left unset, LadderReducingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
LadderAlkylatingAgentStorageCondition
The non-default storage condition for LadderAlkylatingAgent of this experiment after the protocol is completed. If left unset, LadderAlkylatingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Ladder Separation
LadderInjectionVoltageDurationProfile
Voltage profile for Injection. Supports up to 20 steps where each step is 0-200 Second, and 0-6500 volts.
Default Calculation: When LadderInjectionVoltageDurationProfile is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Programmatic Pattern: ({{RangeP[0*Volt, 6500*Volt], RangeP[0*Second, 200*Second]}..} | Automatic) | Null
LadderSeparationVoltageDurationProfile
Default Calculation: When LadderSeparationVoltageDurationProfile is set to Automatic, it will resolve according to each unique set of options applied to SamplesIn.
Programmatic Pattern: ({{RangeP[0*Volt, 6500*Volt], RangeP[0*Minute, 180*Minute]}..} | Automatic) | Null
Standards
IncludeStandards
Indicates if standards should be included in this experiment. Standards contain identified analytes of known size and Relative Migration Times. Standards are used to both ensure reproducibility within and between Experiments and to interpolate the molecular weight of unknown analytes.
Default Calculation: If any Standard-related options are set, then this is set True; otherwise, false.
Standards
Default Calculation: If IncludeStandards is True, Standards is resolved to Model[Sample, StockSolution, "Resuspended CESDS IgG Standard"].
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardStorageCondition
The non-default storage condition for Standards of this experiment after the protocol is completed. If left unset, StandardStorageCondition will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardFrequency
Indicates how many injections per standard should be included in this experiment. Sample, Standard, and Blank injection order are resolved according to InjectionTable.
Default Calculation: When StandardFrequency is set to Automatic and IncludeStandards is True, Set Frequency to FirstAnLast.
Standard Preparation
StandardVolume
Indicates the volume drawn from the standard to the assay tube. Each tube contains a Standard, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Default Calculation: When StandardVolume is set to Automatic and IncludeStandards is True, the volume is calculated to reach 1 milligram/milliliter in the assay tube, based on its composition, by StandardTotalVolume / Standard [Concentration]. If InjectionTable is informed, specified volumes will be used.
StandardTotalVolume
Indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a standard, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Default Calculation: When StandardTotalVolume is set to Automatic and IncludeStandards is True, Set StandardTotalVolume to TotalVolume.
Pattern Description: Greater than or equal to 50 microliters and less than or equal to 200 microliters or Null.
StandardPremadeMasterMix
Indicates if Standards should be mixed with StandardPremadeMasterMix that includes an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When StandardPremadeMasterMix is set to Automatic, it will resolve to True if any of its downstream options is specified.
StandardPremadeMasterMixReagent
The premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When StandardPremadeMasterMixReagent is set to Automatic, it will resolve to the common premade mastermix reagent in samples in .
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardPremadeMasterMixDiluent
Default Calculation: When StandardPremadeMasterMix is set to True and StandardPremadeMasterMixDiluent is set to Automatic, Model[Sample,"Milli-Q water"] will be set as diluent.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardPremadeMasterMixReagentDilutionFactor
Default Calculation: When StandardPremadeMasterMix is set to True and StandardPremadeMasterMixReagentDilutionFactor is set to Automatic, it will be set as the ratio of the total volume to premade mastermix volume.
StandardPremadeMasterMixVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of TotalVolume by StandardPremadeMasterMixReagentDilutionFactor. If StandardPremadeMasterMix is set to False, StandardPremadeMasterMixVolume is Null.
StandardPremadeMasterMixStorageCondition
The non-default storage condition for StandardPremadeMasterMix of this experiment after the protocol is completed. If left unset, StandardPremadeMasterMix will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardInternalReference
The solution containing the reference analyte by which Relative Migration Time is normalized. By default a 10 KDa marker is used. It is highly recommended to include an internal standard in Sample Preparation.
Default Calculation: When StandardPremadeMasterMix is set to False and InternalReference is set to Automatic, StandardInternalReference will be set as Model[Sample, StockSolution"Resuspended CESDS Internal Standard 25X"]. If StandardPremadeMasterMix is set to True, StandardInternalReference is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardInternalReferenceDilutionFactor
Marks how concentrated the internal standard is. For example, Model[Sample, StockSolution"Resuspended CESDS Internal Standard 25X"] is concentrated 25X, hence 2 µL should be added in Sample Preparation when final volume is set to 60 µL.
Default Calculation: When StandardPremadeMasterMix is set to False and StandardInternalReferenceDilutionFactor is set to Automatic, StandardInternalReferenceDilutionFactor will be set to 25. If StandardPremadeMasterMix is set to True, StandardInternalReferenceDilutionFactor is Null.
StandardInternalReferenceVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of TotalVolume by StandardInternalReferenceVolume. When StandardPremadeMasterMix is set to True, StandardInternalReferenceVolume is Null.
StandardConcentratedSDSBuffer
The SDS Buffer used to dilute standards. The final concentration of SDS in this assay must be equal to or greater than 0.5%.
Default Calculation: When StandardPremadeMasterMix is set to False and StandardConcentratedSDSBuffer is set to Automatic, StandardConcentratedSDSBuffer will be set as Model[Sample,"1% SDS in 100mM Tris, pH 9.5"]. If StandardPremadeMasterMix is set to True, StandardConcentratedSDSBuffer is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardConcentratedSDSBufferDilutionFactor
Marks how concentrated the StandardConcentratedSDSBuffer is. For example, a StandardConcentratedSDSBuffer that contains 1% SDS is concentrated 2X and will constitute half the StandardTotalVolume.
Default Calculation: When StandardPremadeMasterMix is set to False and StandardConcentratedSDSBufferDilutionFactor is set to Automatic, StandardConcentratedSDSBufferDilutionFactor will be set to 2. If StandardPremadeMasterMix is set to True, StandardConcentratedSDSBufferDilutionFactor is Null.
StandardDiluent
Default Calculation: When StandardPremadeMasterMix is set to False and Diluent is set to Automatic, Diluent will be set to Model[Sample,"Milli-Q water"]. If StandardPremadeMasterMix is set to True, StandardDiluent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardConcentratedSDSBufferVolume
Default Calculation: When volume is set to Automatic and StandardPremadeMasterMix is set to False, the volume is calculated by the division of StandardTotalVolume by StandardConcentratedSDSBufferDilutionFactor. When StandardPremadeMasterMix is set to True, StandardConcentratedSDSBufferVolume is Null.
StandardSDSBuffer
The SDS Buffer used to dilute the sample. The final concentration of SDS in this assay must be equal or greater than 0.5%.
Default Calculation: When StandardSDSBuffer is set to Automaticit is resolved to Null, so that Concentrated SDSBuffer, if set to Automatic, is used.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardSDSBufferVolume
Default Calculation: When StandardSDSBufferVolume is set to Automatic and StandardSDSBuffer is not Null, the volume is calculated by the difference between the StandardTotalVolume and the volume in the tube that includes the Standard, InternalReference, ReducingAgent, and AlkylatingAgent.
StandardReduction
Default Calculation: When automatic, StandardReduction will set to True if any of the Reduction options are not Null.
StandardReducingAgent
Default Calculation: When StandardPremadeMasterMix is set to False and StandardReducingAgent is set to Automatic, StandardReducingAgent will be set to Model[Sample,"2-mercaptoethanol"]. If StandardPremadeMasterMix is set to True, StandardReducingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardReducingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, and no StandardReducingAgentVolume value is given, concentration is set to 650 mM. If volume is given concentration will be calculated. If StandardPremadeMasterMix is set to True, StandardReducingAgentTargetConcentration is Null
Pattern Description: Greater than 0 molar or greater than or equal to 0 VolumePercent and less than or equal to 100 VolumePercent or Null.
Programmatic Pattern: ((GreaterP[0*Molar] | RangeP[0*VolumePercent, 100*VolumePercent]) | Automatic) | Null
StandardReducingAgentVolume
Default Calculation: When StandardReducingAgentVolume is set to Automatic and StandardPremadeMasterMix is set to False, calculate the volume required to reach StandardReducingAgentTargetConcentration, if set. When StandardReducingAgentVolume is set to Automatic and StandardReducingAgentTargetConcentration is set to Null, StandardReducingAgentVolume is set to Null. When StandardPremadeMasterMix is set to True, StandardReducingAgentVolume is Null.
StandardAlkylation
Indicates if Alkylation should be applied to the Standard. Alkylation of free cysteines is useful in mitigating unexpected protein fragmentation and reproducibility issues.
Default Calculation: When automatic, StandardAlkylation will set to True if any of the Reduction options are not Null.
StandardAlkylatingAgent
Default Calculation: When StandardPremadeMasterMix is set to False and StandardAlkylatingAgent is set to Automatic, StandardAlkylatingAgent will be set to Model[Sample,"250 mM Iodoacetamide"]. If StandardPremadeMasterMix is set to True, StandardAlkylatingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardAlkylatingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, StandardPremadeMasterMix is set to False, and no StandardAlkylatingAgentVolume value is given, concentration is set to 11.5 millimolar. If volume is given concentration will be calculated. If StandardPremadeMasterMix is set to True, StandardAlkylatingAgentTargetConcentration is Null.
StandardAlkylatingAgentVolume
Default Calculation: When StandardAlkylatingAgentVolume is set to Automatic and StandardPremadeMasterMix is set to False, calculate the volume required to reach StandardAlkylatingAgentTargetConcentration, if set. When StandardAlkylatingAgentVolume is set to Automatic and StandardAlkylatingAgentTargetConcentration is set to Null, StandardAlkylatingAgentVolume is set to Null. If StandardPremadeMasterMix is set to True, StandardAlkylatingAgentVolume is Null.
StandardSedimentationSupernatantVolume
The volume of supernatant to transfer to the assay container from the Standard tubes after denaturation and centrifugation. A minimum of 50 µL are required for the analysis to proceed.
Default Calculation: When StandardPelletSedimentation is True and SedimentationSupernatantVolume is set to Automatic, 90% of the TotalVolume is drawn to avoid disturbing the pellet.
StandardInternalReferenceStorageCondition
The non-default storage condition for StandardInternalReference of this experiment after the protocol is completed. If left unset, StandardInternalReferences will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardConcentratedSDSBufferStorageCondition
The non-default storage condition for StandardConcentratedSDSBuffer of this experiment after the protocol is completed. If left unset, StandardConcentratedSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardDiluentStorageCondition
The non-default storage condition for StandardDiluent of this experiment after the protocol is completed. If left unset, StandardDiluent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardSDSBufferStorageCondition
The non-default storage condition for StandardSDSBuffer of this experiment after the protocol is completed. If left unset, StandardSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardReducingAgentStorageCondition
The non-default storage condition for StandardReducingAgent of this experiment after the protocol is completed. If left unset, StandardReducingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardAlkylatingAgentStorageCondition
The non-default storage condition for StandardAlkylatingAgent of this experiment after the protocol is completed. If left unset, StandardAlkylatingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Standard Separation
StandardInjectionVoltageDurationProfile
Voltage profile for Injection. Supports up to 20 steps where each step is 0-200 Second, and 0-6500 volts.
Default Calculation: When StandardInjectionVoltageDurationProfile is set to Automatic and IncludeStandards is True, StandardInjectionVoltageDurationProfile is set to {{4600*Volt,20*Second}}, otherwise, Null.
Programmatic Pattern: ({{RangeP[0*Volt, 6500*Volt], RangeP[0*Second, 200*Second]}..} | Automatic) | Null
StandardSeparationVoltageDurationProfile
Default Calculation: When StandardSeparationVoltageDurationProfile is set to Automatic and IncludeStandards is True, separation time will be set according to whether or not standards are reduced or not, and which Standard is used. If standard is IgG and not reduced, set time to 25 minutes, otherwise set to 35 minutes. For all conditions, voltage is set to 5750 Volts. When IncludeStandards is False, StandardSeparationVoltageDurationProfile is Null.
Programmatic Pattern: ({{RangeP[0*Volt, 6500*Volt], RangeP[0*Minute, 180*Minute]}..} | Automatic) | Null
Blanks
IncludeBlanks
Indicates if standards should be included in this experiment. Standards contain identified analytes of known size and Relative Migration Times that can be used as reference for unknown analytes.
Blanks
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankStorageCondition
The non-default storage condition for Blanks of this experiment after the protocol is completed. If left unset, BlanksStorageCondition will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankFrequency
Indicates how many injections per Blank should be included in this experiment. Sample, Standard, and Blank injection order are resolved according to InjectionTable.
Default Calculation: When BlankFrequency is set to Automatic and IncludeBlanks is True, Set Frequency to FirstAnLast.
Blank Preparation
BlankVolume
Indicates the volume drawn from the blank to the assay tube. Each tube contains a Blank, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Default Calculation: When BlankVolume is set to Automatic and IncludeBlanks is True, the volume is calculated to be 25% of the BlankTotalVolume. When IncludeBlanks is False, BlankVolume is Null. If InjectionTable is informed, specified volumes will be used.
BlankTotalVolume
Indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a standard, an InternalReference, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Default Calculation: When BlankTotalVolume is set to Automatic and IncludeBlanks is True, Set BlankTotalVolume to TotalVolume. When IncludeBlanks is set to False, BlankTotalVolume is Null.
Pattern Description: Greater than or equal to 50 microliters and less than or equal to 200 microliters or Null.
BlankPremadeMasterMix
Indicates if Blanks should be mixed with StandardPremadeMasterMix that includes an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When BlankPremadeMasterMix is set to Automatic, it will resolve to True if any of its downstream options is specified.
BlankPremadeMasterMixReagent
The premade master mix used for CESDS experiment, containing an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Default Calculation: When BlankPremadeMasterMixReagent is set to Automatic, it will resolve to the common premade mastermix reagent in samples in .
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankPremadeMasterMixDiluent
Default Calculation: When BlankPremadeMasterMix is set to True and BlankPremadeMasterMixDiluent is set to Automatic, Model[Sample,"Milli-Q water"] will be set as diluent.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankPremadeMasterMixReagentDilutionFactor
Default Calculation: When PremadeMasterMix is set to True and PremadeMasterMixReagentDilutionFactor is set to Automatic, it will be set as the ratio of the total volume to premade mastermix volume.
BlankPremadeMasterMixVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of TotalVolume by BlankPremadeMasterMixReagentDilutionFactor. If BlankPremadeMasterMix is set to False, BlankPremadeMasterMixVolume is Null.
BlankPremadeMasterMixStorageCondition
The non-default storage condition for BlankPremadeMasterMix of this experiment after the protocol is completed. If left unset, BlankPremadeMasterMix will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankInternalReference
The solution containing the reference analyte by which Relative Migration Time is normalized. By default a 10 KDa marker is used. It is highly recommended to include an internal standard in Sample Preparation.
Default Calculation: When BlankPremadeMasterMix is set to False and InternalReference is set to Automatic, BlankInternalReference will be set as Model[Sample, StockSolution"Resuspended CESDS Internal Standard 25X"]. If BlankPremadeMasterMix is set to True, BlankInternalReference is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankInternalReferenceDilutionFactor
Marks how concentrated the internal standard is. For example, Model[Sample, StockSolution"Resuspended CESDS Internal Standard 25X"] is concentrated 25X, hence 2 µL should be added in Sample Preparation when final volume is set to 60 µL.
Default Calculation: When BlankPremadeMasterMix is set to False and BlankInternalReferenceDilutionFactor is set to Automatic, BlankInternalReferenceDilutionFactor will be set to 25. If BlankPremadeMasterMix is set to True, BlankInternalReferenceDilutionFactor is Null.
BlankInternalReferenceVolume
Default Calculation: When volume is set to Automatic, the volume is calculated by the division of TotalVolume by StandardInternalReferenceVolume. When BlankPremadeMasterMix is set to True, BlankInternalReferenceVolume is Null.
BlankConcentratedSDSBuffer
The SDS Buffer used to dilute blanks. The final concentration of SDS in this assay must be equal to or greater than 0.5%.
Default Calculation: When BlankPremadeMasterMix is set to False and BlankConcentratedSDSBuffer is set to Automatic, BlankConcentratedSDSBuffer will be set as Model[Sample,"1% SDS in 100mM Tris, pH 9.5"]. If BlankPremadeMasterMix is set to True, BlankConcentratedSDSBuffer is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankConcentratedSDSBufferDilutionFactor
Marks how concentrated the BlankConcentratedSDSBuffer is. For example, a BlankConcentratedSDSBuffer that contains 1% SDS is concentrated 2X and will constitute half the BlankTotalVolume.
Default Calculation: When BlankPremadeMasterMix is set to False and BlankConcentratedSDSBufferDilutionFactor is set to Automatic, BlankConcentratedSDSBufferDilutionFactor will be set to 2. If BlankPremadeMasterMix is set to True, BlankConcentratedSDSBufferDilutionFactor is Null.
BlankDiluent
Default Calculation: When BlankPremadeMasterMix is set to False and Diluent is set to Automatic, Diluent will be set to Model[Sample,"Milli-Q water"]. If BlankPremadeMasterMix is set to True, BlankDiluent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankConcentratedSDSBufferVolume
Default Calculation: When volume is set to Automatic and BlankPremadeMasterMix is set to False, the volume is calculated by the division of BlankTotalVolume by BlankConcentratedSDSBufferDilutionFactor. When BlankPremadeMasterMix is set to True, BlankConcentratedSDSBufferVolume is Null.
BlankSDSBuffer
The SDS Buffer used to dilute the sample. The final concentration of SDS in this assay must be equal or greater than 0.5%.
Default Calculation: When BlankSDSBuffer is set to Automaticit is resolved to Null, so that Concentrated SDSBuffer, if set to Automatic, is used.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankSDSBufferVolume
Default Calculation: When BlankSDSBufferVolume is set to Automatic and BlankSDSBuffer is not Null, the volume is calculated by the difference between the BlankTotalVolume and the volume in the tube that includes the Blank, InternalReference, ReducingAgent, and AlkylatingAgent.
BlankReduction
Default Calculation: When automatic, StandardReduction will set to True if any of the Reduction options are not Null.
BlankReducingAgent
Default Calculation: When BlankPremadeMasterMix is set to False and BlankReducingAgent is set to Automatic, BlankReducingAgent will be set to Model[Sample,"2-mercaptoethanol"]. If BlankPremadeMasterMix is set to True, BlankReducingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankReducingAgentTargetConcentration
Default Calculation: When target concentration is set to Automatic, and no BlankReducingAgentVolume value is given, concentration is set to 650 mM. If volume is given concentration will be calculated. If BlankPremadeMasterMix is set to True, BlankReducingAgentTargetConcentration is Null
Pattern Description: Greater than 0 molar or greater than or equal to 0 VolumePercent and less than or equal to 100 VolumePercent or Null.
Programmatic Pattern: ((GreaterP[0*Molar] | RangeP[0*VolumePercent, 100*VolumePercent]) | Automatic) | Null
BlankReducingAgentVolume
Default Calculation: When BlankReducingAgentVolume is set to Automatic and BlankPremadeMasterMix is set to False, calculate the volume required to reach BlankReducingAgentTargetConcentration, if set. When BlankReducingAgentVolume is set to Automatic and BlankReducingAgentTargetConcentration is set to Null, BlankReducingAgentVolume is set to Null. When BlankPremadeMasterMix is set to True, BlankReducingAgentVolume is Null.
BlankAlkylation
Indicates if Alkylation should be applied to the Blank. Alkylation of free cysteines is useful in mitigating unexpected protein fragmentation and reproducibility issues.
Default Calculation: When automatic, BlankAlkylation will set to True if any of the Reduction options are not Null.
BlankAlkylatingAgent
Default Calculation: When BlankPremadeMasterMix is set to False and BlankAlkylatingAgent is set to Automatic, BlankAlkylatingAgent will be set to Model[Sample,"250 mM Iodoacetamide"]. If BlankPremadeMasterMix is set to True, BlankAlkylatingAgent is Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankAlkylatingAgentTargetConcentration
Default Calculation: When BlankPremadeMasterMix is set to False and BlankAlkylatingAgent is set to Automatic, BlankAlkylatingAgent will be set to Model[Sample,"250 mM Iodoacetamide"]. If BlankPremadeMasterMix is set to True, BlankAlkylatingAgent is Null.
BlankAlkylatingAgentVolume
Default Calculation: When target concentration is set to Automatic, BlankPremadeMasterMix is set to False, and no BlankAlkylatingAgentVolume value is given, concentration is set to 11.5 millimolar. If volume is given concentration will be calculated. If BlankPremadeMasterMix is set to True, BlankAlkylatingAgentTargetConcentration is Null.
BlankSedimentationSupernatantVolume
The volume of supernatant to transfer to the assay container from the Blank tubes after denaturation and centrifugation. A minimum of 50 µL are required for the analysis to proceed.
Default Calculation: When BlankPelletSedimentation is True and SedimentationSupernatantVolume is set to Automatic, 90% of the TotalVolume is drawn to avoid disturbing the pellet.
BlankInternalReferenceStorageCondition
The non-default storage condition for BlankInternalReference of this experiment after the protocol is completed. If left unset, BlankInternalReferences will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankConcentratedSDSBufferStorageCondition
The non-default storage condition for BlankConcentratedSDSBuffer of this experiment after the protocol is completed. If left unset, BlankConcentratedSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankDiluentStorageCondition
The non-default storage condition for BlankDiluent of this experiment after the protocol is completed. If left unset, BlankDiluent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankSDSBufferStorageCondition
The non-default storage condition for BlankSDSBuffer of this experiment after the protocol is completed. If left unset, BlankSDSBuffer will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankReducingAgentStorageCondition
The non-default storage condition for BlankReducingAgent of this experiment after the protocol is completed. If left unset, BlankReducingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankAlkylatingAgentStorageCondition
The non-default storage condition for BlankAlkylatingAgent of this experiment after the protocol is completed. If left unset, BlankAlkylatingAgent will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Blank Separation
BlankInjectionVoltageDurationProfile
Voltage profile for Injection. Supports up to 20 steps where each step is 0-200 Second, and 0-6500 volts.
Default Calculation: When BlankInjectionVoltageDurationProfile is set to Automatic and IncludeBlanks is True, BlankInjectionVoltageDurationProfile is set to {{4600*Volt,20*Second}}, otherwise, it is set to Null.
BlankSeparationVoltageDurationProfile
Default Calculation: When BlankSeparationVoltageDurationProfile is set to Automatic, separation time will be set according to whether or not standards are reduced or not, and which Blank is used. If standard is IgG and not reduced, set time to 25 minutes, otherwise set to 35 minutes. For all conditions, voltage is set to 5750 Volts. If IncludeBlanks is False, BlankSeparationVoltageDurationProfile is set to Null.
Programmatic Pattern: ({{RangeP[0*Volt, 6500*Volt], RangeP[0*Minute, 180*Minute]}..} | Automatic) | Null
Model Input
PreparedModelContainer
Indicates the container in which a Model[Sample] specified as input to the experiment function will be prepared.
Default Calculation: If PreparedModelAmount is set to All and when the input model has a product associated with both Amount and DefaultContainerModel populated, automatically set to the DefaultContainerModel value in the product. Otherwise set to Model[Container, Vessel, "2mL Tube"].
PreparedModelAmount
Indicates the amount of a Model[Sample] specified as input to the experiment function that will be prepared in the PreparedModelContainer. When set to All and the input model sample is not preparable, the entire amount of the input model sample that comes from one of the Products is prepared. The selected product must have both Amount and DefaultContainerModel populated, and it must not be a KitProduct. When set to All and the input model is preparable such as water, 1 Milliliter of the input model sample is prepared.
Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All) | Automatic) | Null
Post Experiment
SamplesInStorageCondition
The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Sample Prep Options
Aliquoting
Aliquot
Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
AliquotAmount
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All) | Automatic) | Null
TargetConcentration
The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
TargetConcentrationAnalyte
Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
AssayVolume
Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
ConcentratedBuffer
The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BufferDilutionFactor
The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
BufferDiluent
The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AssayBuffer
The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AliquotSampleStorageCondition
The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
DestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null
AliquotContainer
The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null
AliquotPreparation
Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
ConsolidateAliquots
Sample Preparation
PreparatoryUnitOperations
Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSamplePreparation.
Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.
Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null
Preparatory Incubation
Incubate
Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
IncubationTemperature
Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null
IncubationTime
Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
Mix
Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
MixType
Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
MixUntilDissolved
Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
MaxIncubationTime
Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
IncubationInstrument
Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
AnnealingTime
Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
IncubateAliquotContainer
The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
IncubateAliquotDestinationWell
The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
IncubateAliquot
The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
Preparatory Centrifugation
Centrifuge
Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
CentrifugeInstrument
Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null
CentrifugeIntensity
The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
CentrifugeTime
CentrifugeTemperature
The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
CentrifugeAliquotContainer
The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
CentrifugeAliquotDestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
CentrifugeAliquot
The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
Preparatory Filtering
Filtration
Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
FiltrationType
Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
FilterInstrument
Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null
Filter
The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null
FilterMaterial
The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, AgaroseResin, CobaltResin, Silica, HLB, or AnoporeAlumina or Null.
PrefilterMaterial
The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, AgaroseResin, CobaltResin, Silica, HLB, or AnoporeAlumina or Null.
FilterPoreSize
The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
Pattern Description: 0.008 micrometers, 0.02 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
PrefilterPoreSize
The pore size of the filter; all particles larger than this should be removed during the filtration.
Pattern Description: 0.008 micrometers, 0.02 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
FilterSyringe
Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null
FilterHousing
The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null
FilterIntensity
Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
FilterTime
Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
FilterTemperature
The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
FilterContainerOut
The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
FilterAliquotDestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
FilterAliquotContainer
The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
FilterAliquot
The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
FilterSterile
Protocol Options
Organizational Information
Template
A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null
Name
A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
Post Experiment
MeasureWeight
Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
MeasureVolume
Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
ImageSample
Example Calls
Warnings and Errors
Messages (66)
AlkylatingAgentTargetConcentrationNull (2)
AlkylatingAgentVolumeConcentrationMismatch (1)
AlkylatingAgentVolumeNull (1)
CentrifugationForceSpeedMismatch (1)
CentrifugationForceSpeedNull (1)
ComponentsDontSumToTotalVolume (2)
ConcentratedSDSBufferDilutionFactorNull (1)
DiluentNull (1)
FewerLaddersThanUniqueOptionSets (1)
IncompatibleCartridge (1)
InjectionTableMismatch (2)
InjectionTableReplicatesSpecified (1)
InjectionTableVolumeZero (1)
InsufficientSDSinSample (2)
InternalReferenceDilutionFactorNull (1)
InternalReferenceNull (1)
InternalReferenceVolumeDilutionFactorMismatch (1)
InternalReferenceVolumeNull (1)
InvalidSupernatantVolume (2)
LadderAnalyteMolecularWeightMismatch (1)
LadderAnalytesCompositionMolecularWeightMismatch (1)
LadderCompositionMolecularWeightMismatch (1)
LadderDilutionFactorNull (2)
MissingSampleComposition (2)
MolecularWeightMissing (1)
NoAlkylatingAgentIdentified (2)
NoReducingAgentIdentified (2)
NotEnoughUsesLeftOnCartridge (1)
ObjectDoesNotExist (6)
Do NOT throw a message if we have a simulated container but a simulation is specified that indicates that it is simulated:
Do NOT throw a message if we have a simulated sample but a simulation is specified that indicates that it is simulated:
Throw a message if we have a container that does not exist (ID form):
Throw a message if we have a container that does not exist (name form):
Throw a message if we have a sample that does not exist (ID form):
Throw a message if we have a sample that does not exist (name form):
PremadeMasterMixDiluentNull (1)
PremadeMasterMixDilutionFactorNull (1)
PremadeMasterMixInvalidTotalVolume (1)
PremadeMasterMixReagentNull (1)
PremadeMasterMixVolumeDilutionFactorMismatch (1)
PremadeMasterMixVolumeNull (1)
PreMadeMasterMixWithMakeOwnOptions (1)
ReducingAgentTargetConcentrationNull (2)
ReducingAgentVolumeConcentrationMismatch (1)
TooManyInjectionsCapillaryGelElectrophoresis (1)
Possible Issues
Sample composition
The salt concentration in samples should be <50mM. Higher concentrations will negatively affect sample electrokinetic injection. If samples have higher salt concentrations, please consider desalting with ExperimentSolidPhaseExtraction.
Incompatible reagents
The use the manufacturer's reagents for ConditioningAcid, ConditioningBase, SystemWashSolution, and InternalReference is highly recommended.
Cartridge number of uses
The CESDS-Plus experiment cartridge can be used for up to 500 injections in up to 25 batched. Note that the manufacturer guarantees performance only for the first 100 injections for each cartridge.
Lyophilized reagents
Several of the manufacturer's reagents are delivered lyophilized and need to be resuspended and aliquoted before use in ExperimentCapillaryGelElectrophoresisSDS.
Method Development
Last modified on Mon 18 Aug 2025 13:33:26