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ExperimentUVMelting

ExperimentUVMelting[Samples]⟹Protocol

generates a Protocol object for assessing the dissociation characteristics on the provided Samples during heating and cooling (melting curve analysis) using UV absorbance measurements.

    
ExperimentUVMelting measures the UV absorbance of hybridized nucleic acids across a temperature gradient. Double-stranded nucleic acids like DNA, display an ultraviolet absorption peak at 260 nm which increases upon thermal dissociation of the molecule into complementary single strands, also known as "melting" or "denaturation". In this experiment, absorbance can be monitored during a heating temperature ramp where strands are denatured and a cooling temperature ramp where strands are hybridized. The data generated by this experiment can be used to determine the sample melting point which is characteristic of the sample sequence, length, concentration, sequence mismatch, and solvent conditions.
    

Experimental Principles

    Figure 1.1: Step1: Samples can be incubated, centrifuged, filtered, and/or aliquoted in a compatible container using the shared sample preparation framework. Step2: Prepared samples are transferred and pooled as desired into instrument compatible cuvettes. Samples can then be mixed and incubated using pooled sample options. Step3: Prepared samples are equilibrated to the starting temperature of the assay. Step4: Samples are repeatedly excited by the xenon flash lamp and UV absorbance is read simultaneously for all cuvettes during the temperature ramp. Acquisition intervals are determined by TemperatureResolution option. Steps 3 & 4 are repeated for each heating and cooling cycle. Step5: Samples are tranfered out of cuvettes for storage. Step6: Data is assessed using AnalyzeMeltingPoint to determine the melting temperature of samples.

Instrumentation

    Cary 3500

    Figure 2.1.1: The Cary 3500 is a UV/Vis spectrometer with the capacity to interrogate seven samples and one reference sample simultaneously. The temperature for each pair of sample cuvettes is controlled by Peltier modules for a total of 4 independent temperature zones that can range from -10 Celsius to 100 Celsius. Currently ExperimentUVMelting supports a single temperature profile for all temperature zones. Thermal profiles can be programmed into the instrument to interrogate samples at multiple temperatures. Sample temperature can either be recorded from the cuvette block temperature or by immersion probes. The light source is a xenon flash lamp. The light beam from the lamp is split into different wavelengths ranging from 190 nm to 900 nm and the desired wavelength is isolated using a monochromator. The incident light beam then passes into the sample module where it is split into 8 beams by a fiber optic beam splitter. Each beam excites one sample cuvette and the emitted light is then collected by the instrument detector.

Experiment Options

    Method

    Instrument

    The UV-Vis spectrophotometer to be used for this UVMelting Experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an instrument model that supports all specified options.
    Pattern Description: An object of type or subtype Model[Instrument, Spectrophotometer] or Object[Instrument, Spectrophotometer]
    Programmatic Pattern: ObjectP[{Model[Instrument, Spectrophotometer], Object[Instrument, Spectrophotometer]}] | Automatic

    MaxTemperature

    The high temperature in Celsius of the melting and cooling curves.
    Default Value: 95 degrees Celsius
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 100 degrees Celsius.
    Programmatic Pattern: RangeP[-10*Celsius, 100*Celsius] | Ambient

    MinTemperature

    The low temperature in Celsius of the melting and cooling curves.
    Default Value: 5 degrees Celsius
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 100 degrees Celsius or Null.
    Programmatic Pattern: (RangeP[-10*Celsius, 100*Celsius] | Ambient) | Null

    NumberOfCycles

    The number of repeated melting and cooling cycles to be performed.
    Default Value: 1
    Pattern Description: Greater than or equal to 1 and less than or equal to 5 in increments of 1.
    Programmatic Pattern: RangeP[1, 5, 1]

    EquilibrationTime

    The time in seconds between each melting and cooling cycle.
    Default Value: 5 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 999 minutes.
    Programmatic Pattern: RangeP[0*Minute, 999*Minute]

    TemperatureRampOrder

    The order of temperature ramping to be performed in each cycle.
    Default Value: {Heating, Cooling}
    Pattern Description: {Heating, Cooling} or {Cooling, Heating}
    Programmatic Pattern: ThermodynamicCycleP

    TemperatureResolution

    The amount by which the temperature will be changed between each data point and the next during the melting and/or cooling curves.
    Default Value: 1 degree Celsius
    Pattern Description: Greater than or equal to 1 degree Celsius and less than or equal to 100 degrees Celsius.
    Programmatic Pattern: RangeP[1*Celsius, 100*Celsius]

    TemperatureRampRate

    The rate at which the temperature is changed in the course of one heating and/or cooling cycle.
    Default Value: 1 degree Celsius per minute
    Pattern Description: Greater than or equal to 0.1 degrees Celsius per minute and less than or equal to 40 degrees Celsius per minute.
    Programmatic Pattern: RangeP[0.1*(Celsius/Minute), 40*(Celsius/Minute)]

    Wavelength

    Wavelength of light which should be passed through the cuvette holding the sample in order to measure the sample's absorbance.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Null when MinWavelength or MaxWavelength are set, otherwise resolves to 260 nm for oligomer (DNA/RNA/PNA) samples or to 280 nm for protein samples.
    Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or Null.
    Programmatic Pattern: (RangeP[190*Nanometer, 900*Nanometer] | Automatic) | Null

    MinWavelength

    The lower limit of the wavelength range of light that should be passed through the cuvette holding the sample in order to measure the sample's absorbance. Note that currently the Cary 3500 can only collect 10 wavelengths per scan, and so when specifying a wavelength range, the wavelengths actually measured will be a selection in this range that always includes the MinWavelength, the MaxWavelength, and, if included in the range, 260 Nanometer and 280 Nanometer.
    Default Value: Automatic
    Default Calculation: Automatically resolves to the highest possible setting (900 Nanometer) if MinWavelength is specified, otherwise resolves to Null.
    Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or Null.
    Programmatic Pattern: (RangeP[190*Nanometer, 900*Nanometer] | Automatic) | Null

    MaxWavelength

    The upper limit of the wavelength range of light that should be passed through the cuvette holding the sample in order to measure the sample's absorbance. Note that currently the Cary 3500 can only collect 10 wavelengths per scan, and so when specifying a wavelength range, the wavelengths actually measured will be a selection in this range that always includes the MinWavelength, the MaxWavelength, and, if included in the range, 260 Nanometer and 280 Nanometer.
    Default Value: Automatic
    Default Calculation: Automatically resolves to the lowest possible wavelength (190 Nanometer) if MaxWavelength is specified, otherwise resolves to Null.
    Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or Null.
    Programmatic Pattern: (RangeP[190*Nanometer, 900*Nanometer] | Automatic) | Null

    TemperatureMonitor

    Which device (probe or block) will be used to monitor temperature during the Experiment.
    Default Value: CuvetteBlock
    Pattern Description: CuvetteBlock or ImmersionProbe.
    Programmatic Pattern: CuvetteBlock | ImmersionProbe

    BlankMeasurement

    Indicates if for each pooled sample, a corresponding blank measurement with buffer only will be performed, prior to the absorbance thermodynamics run of the pooled sample.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Blank

    The source used to generate a blank sample whose absorbance is subtracted as background from the absorbance readings of the input sample.
    Default Value: Automatic
    Default Calculation: Automatically set to Null if BlankMeasurement is False, or to the value of AssayVolume if that was specified and BlankMeasurement is True, or Model[Sample, "Milli-Q water"] otherwise.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    NumberOfReplicates

    The number of times to repeat the experiment on each sample or list of samples, if provided. Note that if Aliquot -> True, this indicates the number of times each provided sample will be aliquoted/pooled into a cuvette before each pool is read once.
    Default Value: Null
    Pattern Description: Greater than or equal to 2 in increments of 1 or Null.
    Programmatic Pattern: GreaterEqualP[2, 1] | Null

    Sample Preparation

    NestedIndexMatchingMix

    Indicates if mixing of the pooled samples occur inside the cuvette.
    Default Value: Automatic
    Default Calculation: Automatically set based on whether pooling and/or dilution of the source samples will be performed.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingMixType

    Indicates the style of motion used to mix the pooled samples inside the cuvette.
    Default Value: Automatic
    Default Calculation: Automatically set to Pipette if the cuvette aperture and available tip types allow for mixing by pipette, otherwise set to Invert.
    Pattern Description: Pipette or Invert or Null.
    Programmatic Pattern: ((Pipette | Invert) | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingNumberOfMixes

    The number of times each pooled sample is mixed by pipetting or inversion.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 and less than or equal to 50 in increments of 1 or Null.
    Programmatic Pattern: (RangeP[1, 50, 1] | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingMixVolume

    The volume of each pooled sample that is pipetted up and down to mix.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 50 milliliters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 50*Milliliter] | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingIncubate

    Indicates if thermal incubation of the pooled samples occur prior to measurement.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    PooledIncubationTime

    Duration for which the pooled samples are thermally incubated prior to measurement.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingIncubationTemperature

    Temperature at which the pooled samples are thermally incubated prior to measurement.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to 22 degrees Celsius and less than or equal to 90 degrees Celsius or Null.
    Programmatic Pattern: ((RangeP[22*Celsius, 90*Celsius] | Ambient) | Automatic) | Null
    Index Matches to: experiment samples

    NestedIndexMatchingAnnealingTime

    Duration for which the pooled samples remain in the thermal incubation instrument before being removed, allowing the system to settle to room temperature after the PooledIncubationTime has passed.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    Sample Storage

    ContainerOut

    The desired container generated samples should be transferred into by the end of the experiment, with indices indicating grouping of samples in the same plates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the total volume of the pooled samples.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null
    Index Matches to: experiment samples

    SamplesOutStorageCondition

    The non-default conditions under which any new samples generated by this experiment should be stored after the protocol is completed. If left unset, the new samples will be stored according to their Models' DefaultStorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null
    Index Matches to: experiment samples

Sample Prep Options

    Sample Preparation

    PreparatoryUnitOperations

    Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSampleManipulation.
    Default Value: Null
    Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.
    Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null

    PreparatoryPrimitives

    Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSampleManipulation.
    Default Value: Null
    Pattern Description: List of one or more a primitive with head Define, Transfer, Mix, Aliquot, Consolidation, FillToVolume, Incubate, Filter, Wait, Centrifuge, or Resuspend entries or Null.
    Programmatic Pattern: {SampleManipulationP..} | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: (MixTypeP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: (ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubateAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Ambient | RangeP[-10*Celsius, 40*Celsius]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: (FiltrationTypeP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: (FilterSizeP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: (FilterSizeP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Alternatives[GreaterEqualP[4*Celsius]]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)]) | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: (ObjectP[IdentityModelTypes] | Automatic) | Null
    Index Matches to: experiment samples
    Nested Index Matches to: experiment samples

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 20*Liter] | Automatic) | Null
    Index Matches to: experiment samples

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentrationFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[1, 1] | Automatic) | Null
    Index Matches to: experiment samples

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, BacteriaIncubation, MammalianIncubation, TissueCultureCellsIncubation, MicrobialCellsIncubation, MicrobialCellsShakingIncubation, YeastCellsIncubation, YeastCellsShakingIncubation, ViralIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null
    Index Matches to: experiment samples

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries or Null.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Protocol Options

    Organizational Information

    Template

    A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
    Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

    Name

    A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String | Null

    Post Experiment

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Example Calls

    Pooled Samples

    Measure absorbance of two sets of reverse complementary DNA strands. For mixture 1 pool 200uL of both Sample1Sense and Sample1Antisense and for mixture 2 pool 300uL of Sample2Sense and Sample2Antisense:
    Measure absorbance of any number of pooled samples:

    Pooled Sample Mixing and Incubation

    Apply mixing and incubation to pooled samples just before absorbance measurement:

Preferred Input Containers

    These cuvette models are compatible with the instrument.

Data Processing

    Calculate the melting temperature of the sample for each heating and cooling cycle. Note that these are not the same due to a high ramp rate. In order to ensure the heating and cooling curves look the same, it is important to ramp the temperature up and down slowly:

Warnings and Errors

    Messages  (28)

    BlanksContainSamplesIn  (1)

    An input sample cannot also be specified as a Blank:

    ContainerOutMismatchedIndex  (1)

    When specifying ContainerOut using the index syntax, each :

    ContainerOutStorageConditionConflict  (2)

    If samples are to be stored in a plate, there can be only one storage condition:

    If samples are to be stored in a plate, there can be only one storage condition (index-matched ContainerOut case):

    ContainerOverOccupied  (1)

    The specified ContainerOut cannot request more positions than the container has:

    DiscardedSamples  (1)

    If the given input samples are discarded, they cannot be measured:

    IncompatibleCuvette  (1)

    The container in which the samples are to be pooled and measured (AliquotContainer) has to be compatible with the instrument (for example, it has to be made of quartz):

    IncompatibleSpectrophotometer  (1)

    Only spectrophotometers with UV/Vis capabilities can be used for this experiment:

    InputContainsTemporalLinks  (1)

    Throw a message if given a temporal link:

    InstrumentPrecision  (1)

    The precision of the Wavelength cannot be not more than 0.1 Nanometer, the maximum precision achievable by the ECL spectrophotometers:

    InvalidWavelengthRange  (1)

    MinWavelength must be smaller than MaxWavelength:

    MismatchingNestedIndexMatchingdMixOptions  (1)

    If the NestedIndexMatchingMix boolean option is turned to False, other mixing options for the pool cannot be specified:

    MismatchingNestedIndexMatchingIncubateOptions  (1)

    If the NestedIndexMatchingIncubate boolean option is turned to False, other incubation options for the pool(s) cannot be specified:

    MismatchingWavelengthOptions  (1)

    If the Wavelength is specified, neither MinWavelength or MaxWavelength can be specified as well:

    NestedIndexMatchingMixVolumeNotCompatibleWithMixType  (2)

    If NestedIndexMatchingMixType is specified to Invert, NestedIndexMatchingMixVolume cannot be set to a volume:

    If NestedIndexMatchingMixType is specified to Pipette, NestedIndexMatchingMixVolume cannot be set to Null:

    NestedIndexMatchingVolumeBelowMinThreshold  (1)

    A warning is thrown if the total volume of samples and buffers is smaller than the recommended minimum volume of the AliquotContainer specified:

    NestedIndexMatchingVolumeOutOfRange  (2)

    Samples with assay volume (total volume of samples and buffers inside a particular pool) smaller than the minimum working range of the smallest cuvette cannot be reliably measured:

    The assay volume (total volume of samples and buffers inside a particular pool) has to fall within the working range of an available cuvette:

    NoMixingDespiteAliquotting  (1)

    A warning is thrown if samples are being aliquotted but NestedIndexMatchingMix is set to False:

    NonLiquidSample  (1)

    If the given input sample is Solid, it cannot be measured:

    NumberOfReplicatesRequiresAliquoting  (1)

    If NumberOfReplicates is set, Aliquot must not be set to False:

    SamplesOutStorageConditionConflict  (1)

    If SamplesOutStorageCondition is set to Disposal, ContainerOut can't be specified:

    TooManyUVMeltingSamples  (1)

    The number of samples or sample pools to be measured cannot exceed the number of available slots in the cuvette block of the instrument:

    TransferRequired  (1)

    A warning is thrown if a single sample input is given whose container does not fit on the deck and the sample will be transferred into a cuvette prior to measurement:

    UnableToBlank  (1)

    BlankMeasurement can only be set to True if we're using an AliquotContainer to mix the input sample(s) with buffer:

    UVMeltingIncompatibleBlankOptions  (2)

    If BlankMeasurement -> False, then Blank must be Null:

    If BlankMeasurement -> True, then Blank cannot be Null:

Possible Issues

    High ramp rate

    When the ramp rate is too high, the recorded temperature from the cuvette block may not reflect the actual temperature of the samples inside the cuvettes.

    Constant absorbance across temperature range

    If the sample does not have structure-dependent changes in absorbance at the specified wavelength or within the wavelength range, the measured absorbance may be constant across a large temperature.

    Unable to determine melting point

    If the temperature range is not large enough, the melting point may not be conclusively determined.
Last modified on Mon 19 Dec 2022 16:52:51
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