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ExperimentAlphaScreen

ExperimentAlphaScreen[Samples]⟹Protocol

generates a Protocol object for AlphaScreen measurement of the Samples. The samples should contain analytes with acceptor and donor AlphaScreen beads. Upon laser excitation at 680nm to the donor AlphaScreen beads, singlet Oxygen is produced and diffused to the acceptor AlphaScreen beads only when the two beads are in close proximity. The acceptor AlphaScreen beads react with the singlet Oxygen and emit light signal at 570nm which is captured by a detector in plate reader. A plate which contains the AlphaScreen beads and analytes loaded can be provided as input and the plate will be excited directly and measured in the plate reader. Alternatively, prepared samples can be provided and transferred to an assay plate for AlphaScreen measurement.

    
Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen is used to study biological interactions between two different molecules that are conjugated with donor and acceptor beads respectively. Upon laser excitation (~680nm), a photosensitizer in the donor beads converts ambient oxygen to an excited singlet state. When the donor and acceptor beads are in close proximity due to the interaction between two molecules, the excited oxygen can diffuse to the acceptor beads and trigger a cascade of chemical reactions. The reactions produce a wavelength of light (~570nm) that is captured by a detector. The ExperimentAlphaScreen measures the signal from samples that contain all the analytes and beads prepared prior to the AlphaScreen experiment. To prepare the analytes and beads, see our ExperimentBioconjugation and ExperimentSampleManipulation.
    

Experimental Principles

    Figure 1.1: Procedural overview of an AlphaScreen experiment. Step 1: Samples are transferred onto a microplate. If a prepared plate is given, Step 1 is skipped. Step 2: The plate is loaded into the plate reader. Step 3: The plate reader illuminates the samples at 680 nm excitation wavelength and captures emission intensity at 570 nm wavelength.

Instrumentation

    CLARIOstar

    Figure 2.1.1: The CLARIOstar microplate reader is equipped with an AlphaScreen detecting module, which consists of a dedicated laser that produces high-intensity light at 680nm.

Experiment Options

    General

    PreparedPlate

    Indicates if a prepared plate is provided for AlphaScreen measurement. The prepared plate contains the analytes, acceptor and donor AlphaScreen beads that are ready to be excited by AlphaScreen laser for luminescent AlphaScreen measurement in a plate reader. If the 'PreparedPlate' is False, the samples that contain all the components will be transferred to an assay plate for AlphaScreen measurement.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    Instrument

    The plate reader for the signal measurement in AlphaScreen.
    Default Value: Model[Instrument, PlateReader, CLARIOstar]
    Default Calculation: Automatically set to a plate reader model that is available at the time of experiment.
    Pattern Description: An object of type or subtype Model[Instrument, PlateReader] or Object[Instrument, PlateReader]
    Programmatic Pattern: ObjectP[{Model[Instrument, PlateReader], Object[Instrument, PlateReader]}]

    AssayPlateModel

    The plate where the samples are transferred and measured in the plate reader.
    Default Value: Automatic
    Default Calculation: Automatic resolves the plate where the 'AlphaAssayVolume' is within the volume limits. If multiple plate formats support the 'AlphaAssayVolume', automatic resolves to a half area 96-well plate if the number of samples is less than 96, or resolves to a 384-well plate if the number of samples is more than 96. If the 'AlphaAssayVolume' is also set to Automatic, automatic resolves to either a half are 96-well or 384-well plate based on the number of samples as described before.
    Pattern Description: An object of type or subtype Model[Container, Plate] or Null.
    Programmatic Pattern: (ObjectP[{Model[Container, Plate]}] | Automatic) | Null

    WorkCell

    Indicates the work cell that this primitive will be run on if Preparation->Robotic.
    Default Value: Automatic
    Default Calculation: Automatically set to STAR if Preparation->Robotic.
    Pattern Description: STAR, bioSTAR, or microbioSTAR or Null.
    Programmatic Pattern: ((STAR | bioSTAR | microbioSTAR) | Automatic) | Null

    Preparation

    Indicates if this unit operation is carried out primarily robotically or manually. Manual unit operations are executed by a laboratory operator and robotic unit operations are executed by a liquid handling work cell.
    Default Value: Automatic
    Pattern Description: Manual or Robotic.
    Programmatic Pattern: PreparationMethodP | Automatic

    AlphaScreen Sample Preparation

    AlphaAssayVolume

    The total volume that each sample is transferred to the assay plate for luminescent AlphaScreen measurement.
    Default Value: Automatic
    Default Calculation: Automatic resolves to the recommended fill volume of the 'AssayPlateModel'. If the 'AssayPlateModel' is also set to Automatic, automatic resolves to 100 Microliter.
    Pattern Description: Greater than or equal to 0 microliters and less than or equal to 300 microliters or Null.
    Programmatic Pattern: (RangeP[0*Microliter, 300*Microliter] | Automatic) | Null

    NumberOfReplicates

    The number of wells each sample is aliquoted into and read independently.
    Default Value: Null
    Pattern Description: Greater than or equal to 0 and less than or equal to 384 in increments of 1 or Null.
    Programmatic Pattern: RangeP[0, 384, 1] | Null

    Optics Optimization

    OpticsOptimizationTemplate

    A previous protocol that contains the optimized gain and focal height values for the AlphaScreen measurement. The gain and focal height values from the previous protocol are taken and used in the current protocol with no further optimization.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Protocol, AlphaScreen] or Null.
    Programmatic Pattern: ObjectP[Object[Protocol, AlphaScreen]] | Null

    OptimizeGain

    Indicates if luminescence gain value is optimized before the plate reader measurement. The gain value is set so that the signal from the optimization samples would reach the 'TargetSaturationPercentage' of the plate reader's dynamic range. Each optimization sample can only be used once for the optimization, because the signal from the AlphaScreen beads reduces after repeated excitation. The gain optimization is performed by measuring one optimization sample at once and increasing or decreasing the gain value until the 'TargetSaturationPercentage' is reached.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any gain optimization parameters are specified. Otherwise set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    GainOptimizationSamples

    When optimizing gain, the list of samples used for gain optimization. If PreparedPlate->False, one optimization sample should be specified and the sample is aliquoted equal to the 'NumberOfGainOptimizations' option. If PreparedPlate->True, the optimization samples should be in the provided plate. Each aliquot is used only once to measure signal and adjust the gain.
    Default Value: Automatic
    Default Calculation: Automatically set to the first input sample if OptimizeGain is True.
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or list of one or more an object of type or subtype Object[Sample] or a prepared sample or Null entries or Null.
    Programmatic Pattern: (((ObjectP[Object[Sample]] | _String) | {((ObjectP[Object[Sample]] | _String) | Null)..}) | Automatic) | Null

    TargetSaturationPercentage

    When optimizing gain, the gain is set so that the signal from the optimization samples returns the 'TargetSaturationPercentage' of the plate reader's dynamic range.
    Default Value: Automatic
    Default Calculation: Automatically set to 90 Percent if any of the gain optimization parameters is specified.
    Pattern Description: Greater than or equal to 1 percent and less than or equal to 99 percent or Null.
    Programmatic Pattern: (RangeP[1*Percent, 99*Percent] | Automatic) | Null

    NumberOfGainOptimizations

    When optimizing gain, the number of the optimization samples aliquoted onto the assay plate for gain optimization. Each aliquot is used only once to measure signal and adjust the gain.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 if any of the gain optimization parameters is specified.
    Pattern Description: Greater than or equal to 0 and less than or equal to 96 in increments of 1 or Null.
    Programmatic Pattern: (RangeP[0, 96, 1] | Automatic) | Null

    StartingGain

    When optimizing gain, the initial gain value to begin the gain optimization. If the readout from the current gain value is lower/higher than the 'TargetSaturationPercentage', the next gain value for the optimization will be increased/decreased.
    Default Value: Automatic
    Default Calculation: Automatically set to 3600 Microvolt if any of the gain optimization parameters is specified.
    Pattern Description: Greater than or equal to 1 microvolt and less than or equal to 4095 microvolts or Null.
    Programmatic Pattern: (RangeP[1*Microvolt, 4095*Microvolt] | Automatic) | Null

    OptimizeFocalHeight

    Indicates if focal height value is optimized prior to the gain optimization before the plate reader measurement. The focal height value is set so that the signal from the optimization samples would reach its maximum. Each optimization sample is used only once for the optimization.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any focal height optimization parameters are specified. Otherwise set to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    FocalHeightOptimizationSamples

    When optimizing focal height, the list of samples used for focal height optimization. If PreparedPlate->False, one optimization sample should be specified and the sample is aliquoted equal to the 'NumberOfFocalHeightOptimizations' option. If PreparedPlate->True, the optimization samples should be in the provided plate. Each aliquot is used only once to measure signal and adjust the focal height.
    Default Value: Automatic
    Default Calculation: Automatically set to the first input sample if OptimizeFocalHeight is True.
    Pattern Description: An object of type or subtype Object[Sample] or a prepared sample or list of one or more an object of type or subtype Object[Sample] or a prepared sample or Null entries or Null.
    Programmatic Pattern: (((ObjectP[Object[Sample]] | _String) | {((ObjectP[Object[Sample]] | _String) | Null)..}) | Automatic) | Null

    FocalHeightStep

    When optimizing focal height, the step size that the focal height is increased or decreased after measuring each optimization sample.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 Millimeter if any of the focal height optimizatio parameters are set.
    Pattern Description: Greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: (RangeP[0*Millimeter, 25*Millimeter] | Automatic) | Null

    NumberOfFocalHeightOptimizations

    When optimizing focal height, the number of the optimization samples is aliquoted onto the assay plate for focal height optimization. Each aliquot is used only once to measure signal and adjust the focal height.
    Default Value: Automatic
    Default Calculation: Automatically set to 3 if any of the focal height optimizatio parameters are set.
    Pattern Description: Greater than or equal to 0 and less than or equal to 96 or Null.
    Programmatic Pattern: (RangeP[0, 96] | Automatic) | Null

    StartingFocalHeight

    When optimizing focal height, the initial focal height to begin the focal height optimization. An optimization sample is first measured using the 'StartingFocalHeight'. The focal height is then increased by a 'FocalHeightStep' and applied to the next optimization sample. If the new signal readout is less than the previous one, the focal height is decreased by a 'FocalHeightStep' from the previous value. Otherwise, the focal height is increased again stepwise. The focal height that leads to the maximum signal is selected.
    Default Value: Automatic
    Default Calculation: Automatically set to 11 Millimeter if any of the focal height optimization parameters are set.
    Pattern Description: Greater than or equal to 0 millimeters and less than or equal to 25 millimeters or Null.
    Programmatic Pattern: (RangeP[0*Millimeter, 25*Millimeter] | Automatic) | Null

    OpticsOptimizationSampleStorage

    The non-default conditions under which the unused 'GainOptimizationSamples' and 'FocalHeightOptimizationSamples' of this experiment should be stored after the protocol is completed. If left unset, 'GainOptimizationSamples' and 'FocalHeightOptimizationSamples' will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null

    Optics

    ReadTemperature

    The temperature at which the measurement will be taken in the plate reader.
    Default Value: 37 degrees Celsius
    Pattern Description: Ambient or greater than or equal to 25 degrees Celsius and less than or equal to 45 degrees Celsius.
    Programmatic Pattern: RangeP[$AmbientTemperature, 45*Celsius] | Ambient

    ReadEquilibrationTime

    The length of time for which the assay plates should equilibrate at the assay temperature in the plate reader before being read.
    Default Value: 2 minutes
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 2 hours.
    Programmatic Pattern: RangeP[0*Minute, 2*Hour]

    PlateReaderMix

    Indicates if samples should be mixed inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other plate reader mix options are specified.
    Pattern Description: Automatic or True or False.
    Programmatic Pattern: (BooleanP | Automatic) | Automatic

    PlateReaderMixTime

    The amount of time samples should be mixed inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to 30 second if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 1 second and less than or equal to 1 hour or Null.
    Programmatic Pattern: (RangeP[1*Second, 1*Hour] | Automatic) | Null

    PlateReaderMixRate

    The rate at which the plate is agitated inside the plate reader chamber before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to 700 RPM if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Greater than or equal to 100 revolutions per minute and less than or equal to 700 revolutions per minute or Null.
    Programmatic Pattern: (RangeP[100*RPM, 700*RPM] | Automatic) | Null

    PlateReaderMixMode

    The pattern of motion which should be employed to shake the plate before the samples are read.
    Default Value: Automatic
    Default Calculation: Automatically set to DoubleOrbital if PlateReaderMix is True and any other plate reader mix options are specified.
    Pattern Description: Orbital, DoubleOrbital, or Linear or Null.
    Programmatic Pattern: (MechanicalShakingP | Automatic) | Null

    Gain

    The gain which should be applied to the signal reaching the primary detector photomultiplier tube (PMT). This is specified as a direct voltage. If it sets to Automatic, the gain is set from the result of OptimizeGain or a previous protocol.
    Default Value: Automatic
    Default Calculation: Automatic resolves to 3600 Microvolt if OptimizeGain->False.
    Pattern Description: Automatic or greater than or equal to 1 microvolt and less than or equal to 4095 microvolts.
    Programmatic Pattern: (RangeP[1*Microvolt, 4095*Microvolt] | Automatic) | Automatic

    FocalHeight

    The distance from the focal point to the lens that collect the light to the primary detector photomultiplier tube (PMT). If it sets to Automatic, the focal height is set from the result of OptimizeFocalHeight.
    Default Value: Automatic
    Default Calculation: Automatic resolves to 11 Millimeter if OptimizeFocalHeight->False.
    Pattern Description: Automatic or greater than or equal to 0 millimeters and less than or equal to 25 millimeters.
    Programmatic Pattern: (RangeP[0*Millimeter, 25*Millimeter] | Automatic) | Automatic

    SettlingTime

    The time between the movement of the plate and the beginning of the measurement. It reduces potential vibration of the samples in plate due to the stop and go motion of the plate carrier.
    Default Value: 0 milliseconds
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 1 second.
    Programmatic Pattern: RangeP[0*Second, 1*Second]

    ExcitationTime

    The time that the samples will be excited by the light source and the singlet Oxygen is generated.
    Default Value: 80 milliseconds
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 1 second.
    Programmatic Pattern: RangeP[0.01*Second, 1*Second]

    DelayTime

    The time between end of excitation and start of the emission signal integration. It allows the singlet Oxygen to react with the acceptor beads and reduces potential auto-fluorescent noise generated by biomolecular components.
    Default Value: 120 milliseconds
    Pattern Description: Greater than or equal to 0 seconds and less than or equal to 2 seconds.
    Programmatic Pattern: RangeP[0*Second, 2*Second]

    IntegrationTime

    The amount of time for which the emission signal is integrated.
    Default Value: 300 milliseconds
    Pattern Description: Greater than or equal to 0.01 seconds and less than or equal to 2 seconds.
    Programmatic Pattern: RangeP[0.01*Second, 2*Second]

    ExcitationWavelength

    The excitation wavelength determined by the AlphaScreen laser.
    Default Value: 680 nanometers
    Pattern Description: Greater than or equal to 0 nanometers and less than or equal to 1000 nanometers in increments of 1 nanometer.
    Programmatic Pattern: RangeP[0*Nanometer, 1000*Nanometer, 1*Nanometer]

    EmissionWavelength

    The emission wavelength determined by the optical filter.
    Default Value: 570 nanometers
    Pattern Description: Greater than or equal to 0 nanometers and less than or equal to 1000 nanometers in increments of 1 nanometer.
    Programmatic Pattern: RangeP[0*Nanometer, 1000*Nanometer, 1*Nanometer]

    RetainCover

    Indicates if the plate seal or lid on the assay container should not be taken off during measurement to decrease evaporation. It is strongly recommended not to retain a cover because AlphaScreen can only read from top.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

    ReadDirection

    Indicates the order in which wells should be read by specifying the plate path the instrument should follow when measuring signal. Default sets to Row, where the plate is read from left to right for each row.
    Default Value: Row
    Pattern Description: Row, Column, SerpentineRow, or SerpentineColumn.
    Programmatic Pattern: ReadDirectionP

    Sample Handling

    MoatWells

    Indicates if moat wells are set to decrease evaporation from the assay samples during the experiment.
    Default Value: Automatic
    Default Calculation: Automatically set to True if any other moat options are specified.
    Pattern Description: Automatic or True or False or Null.
    Programmatic Pattern: ((BooleanP | Automatic) | Automatic) | Null

    MoatSize

    Indicates the number of concentric perimeters of wells which should be filled with MoatBuffer in order to decrease evaporation from the assay samples during the run.
    Default Value: Automatic
    Default Calculation: Automatically set to 1 if any other moat options are specified. Resolves Null if MoatWells is False.
    Pattern Description: Greater than or equal to 0 in increments of 1 or Null.
    Programmatic Pattern: (GreaterEqualP[0, 1] | Automatic) | Null

    MoatVolume

    Indicates the volume which should be added to each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to the minimum volume of the assay plate if any other moat options are specified.
    Pattern Description: Greater than or equal to 0 microliters or Null.
    Programmatic Pattern: (GreaterEqualP[0*Microliter] | Automatic) | Null

    MoatBuffer

    Indicates the buffer which should be used to fill each moat well.
    Default Value: Automatic
    Default Calculation: Automatically set to Model[Sample,"Milli-Q water"] if any other moat options are specified.]
    Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Object[Sample], Model[Sample]}] | _String) | Automatic) | Null

    Post Experiment

    SamplesInStorageCondition

    The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
    Default Value: Null
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: (Alternatives[SampleStorageTypeP | Disposal]) | Null
    Index Matches to: experiment samples

    StoreMeasuredPlates

    Indicate if the assay plates are stored after the experiment is completed. If it sets to True, the plates are stored according to the storage condition of the samples after measurement. If it sets to False, the plates are discarded after measurement.
    Default Value: False
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP

Sample Prep Options

    Sample Preparation

    PreparatoryUnitOperations

    Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSampleManipulation.
    Default Value: Null
    Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.
    Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null

    PreparatoryPrimitives

    Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSampleManipulation.
    Default Value: Null
    Pattern Description: List of one or more a primitive with head Define, Transfer, Mix, Aliquot, Consolidation, FillToVolume, Incubate, Filter, Wait, Centrifuge, or Resuspend entries or Null.
    Programmatic Pattern: {SampleManipulationP..} | Null

    Preparatory Incubation

    Incubate

    Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    IncubationTemperature

    Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
    Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null
    Index Matches to: experiment samples

    IncubationTime

    Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    Mix

    Indicates if this sample should be mixed while incubated, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    MixType

    Indicates the style of motion used to mix the sample, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
    Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
    Programmatic Pattern: (MixTypeP | Automatic) | Null
    Index Matches to: experiment samples

    MixUntilDissolved

    Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    MaxIncubationTime

    Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
    Pattern Description: Greater than or equal to 1 minute and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[1*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    IncubationInstrument

    The instrument used to perform the Mix and/or Incubation, prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
    Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
    Programmatic Pattern: (ObjectP[Join[MixInstrumentModels, MixInstrumentObjects]] | Automatic) | Null
    Index Matches to: experiment samples

    AnnealingTime

    Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than or equal to 0 minutes and less than or equal to 72 hours or Null.
    Programmatic Pattern: (RangeP[0*Minute, $MaxExperimentTime] | Automatic) | Null
    Index Matches to: experiment samples

    IncubateAliquotContainer

    The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples

    IncubateAliquotDestinationWell

    The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples

    IncubateAliquot

    The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples

    Preparatory Centrifugation

    Centrifuge

    Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    CentrifugeInstrument

    The centrifuge that will be used to spin the provided samples prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeIntensity

    The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeTime

    The amount of time for which the SamplesIn should be centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeTemperature

    The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
    Default Value: Automatic
    Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
    Programmatic Pattern: ((Ambient | RangeP[-10*Celsius, 40*Celsius]) | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeAliquotContainer

    The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples

    CentrifugeAliquot

    The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples

    Preparatory Filtering

    Filtration

    Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
    Default Value: Automatic
    Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    FiltrationType

    The type of filtration method that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
    Pattern Description: PeristalticPump, Centrifuge, Vacuum, Syringe, or AirPressure or Null.
    Programmatic Pattern: (FiltrationTypeP | Automatic) | Null
    Index Matches to: experiment samples

    FilterInstrument

    The instrument that should be used to perform the filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
    Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null
    Index Matches to: experiment samples

    Filter

    The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
    Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
    Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null
    Index Matches to: experiment samples

    FilterMaterial

    The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null
    Index Matches to: experiment samples

    PrefilterMaterial

    The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, Silica, or HLB or Null.
    Programmatic Pattern: (FilterMembraneMaterialP | Automatic) | Null
    Index Matches to: experiment samples

    FilterPoreSize

    The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
    Default Value: Automatic
    Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: (FilterSizeP | Automatic) | Null
    Index Matches to: experiment samples

    PrefilterPoreSize

    The pore size of the filter; all particles larger than this should be removed during the filtration.
    Default Value: Automatic
    Default Calculation: By default, no prefiltration is performed on samples, even when Filter->True.
    Pattern Description: 0.008 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
    Programmatic Pattern: (FilterSizeP | Automatic) | Null
    Index Matches to: experiment samples

    FilterSyringe

    The syringe used to force that sample through a filter.
    Default Value: Automatic
    Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    FilterHousing

    The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
    Default Value: Automatic
    Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
    Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
    Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null
    Index Matches to: experiment samples

    FilterIntensity

    The rotational speed or force at which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
    Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
    Index Matches to: experiment samples

    FilterTime

    The amount of time for which the samples will be centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than 0 minutes or Null.
    Programmatic Pattern: (GreaterP[0*Minute] | Automatic) | Null
    Index Matches to: experiment samples

    FilterTemperature

    The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
    Default Value: Automatic
    Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
    Pattern Description: Greater than or equal to 4 degrees Celsius or Null.
    Programmatic Pattern: ((Alternatives[GreaterEqualP[4*Celsius]]) | Automatic) | Null
    Index Matches to: experiment samples

    FilterContainerOut

    The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples

    FilterAliquotDestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or Null.
    Programmatic Pattern: (WellPositionP | Automatic) | Null
    Index Matches to: experiment samples

    FilterAliquotContainer

    The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Container] or {Index, Container} or Null.
    Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
    Index Matches to: experiment samples

    FilterAliquot

    The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
    Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | All) | Automatic) | Null
    Index Matches to: experiment samples

    FilterSterile

    Indicates if the filtration of the samples should be done in a sterile environment.
    Default Value: Automatic
    Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null
    Index Matches to: experiment samples

    Aliquoting

    Aliquot

    Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
    Default Value: Automatic
    Pattern Description: True or False.
    Programmatic Pattern: BooleanP | Automatic
    Index Matches to: experiment samples

    AliquotAmount

    The amount of a sample that should be transferred from the input samples into aliquots.
    Default Value: Automatic
    Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
    Pattern Description: All or Count or Count or Mass or Volume or Null.
    Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All) | Automatic) | Null
    Index Matches to: experiment samples

    TargetConcentration

    The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
    Default Value: Automatic
    Default Calculation: Automatically calculated based on aliquot and buffer volumes.
    Pattern Description: Greater than 0 molar or greater than 0 grams per liter or Null.
    Programmatic Pattern: ((GreaterP[0*Molar] | GreaterP[0*(Gram/Liter)]) | Automatic) | Null
    Index Matches to: experiment samples

    TargetConcentrationAnalyte

    The substance whose final concentration is attained with the TargetConcentration option.
    Default Value: Automatic
    Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
    Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
    Programmatic Pattern: (ObjectP[IdentityModelTypes] | Automatic) | Null
    Index Matches to: experiment samples

    AssayVolume

    The desired total volume of the aliquoted sample plus dilution buffer.
    Default Value: Automatic
    Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
    Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
    Programmatic Pattern: (RangeP[1*Microliter, 20*Liter] | Automatic) | Null
    Index Matches to: experiment samples

    ConcentratedBuffer

    The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
    Pattern Description: Greater than or equal to 1 or Null.
    Programmatic Pattern: (GreaterEqualP[1] | Automatic) | Null
    Index Matches to: experiment samples

    BufferDiluent

    The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    AssayBuffer

    The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
    Default Value: Automatic
    Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
    Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
    Programmatic Pattern: ((ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic) | Null
    Index Matches to: experiment samples

    AliquotSampleStorageCondition

    The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Default Value: Automatic
    Pattern Description: {AmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
    Programmatic Pattern: ((SampleStorageTypeP | Disposal) | Automatic) | Null
    Index Matches to: experiment samples

    DestinationWell

    The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
    Default Value: Automatic
    Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
    Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
    Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null

    AliquotContainer

    The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
    Default Value: Automatic
    Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
    Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
    Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null

    AliquotPreparation

    Indicates the desired scale at which liquid handling used to generate aliquots will occur.
    Default Value: Automatic
    Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
    Pattern Description: Manual or Robotic or Null.
    Programmatic Pattern: (PreparationMethodP | Automatic) | Null

    ConsolidateAliquots

    Indicates if identical aliquots should be prepared in the same container/position.
    Default Value: Automatic
    Pattern Description: False or Null.
    Programmatic Pattern: (False | Automatic) | Null

Protocol Options

    Organizational Information

    Template

    A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
    Default Value: Null
    Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
    Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null

    Name

    A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
    Default Value: Null
    Pattern Description: A string or Null.
    Programmatic Pattern: _String | Null

    Post Experiment

    MeasureWeight

    Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    MeasureVolume

    Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

    ImageSample

    Indicates if any samples that are modified in the course of the experiment should be freshly imaged after running the experiment. Please note that public samples are imaged regardless of the value of this option.
    Default Value: Automatic
    Pattern Description: True or False or Null.
    Programmatic Pattern: (BooleanP | Automatic) | Null

Example Calls

    Assay Plate Model

    To transfer samples into a plate model supported by the experiment, run:
    ExperimentAlphaScreen can take in a plate where the analytes, acceptor and donor AlphaScreen beads are already mixed and ready for luminescence measurement:

    Plate Reader Mixing

    The assay plate can be mixed during readings by specifying any of the PlateReaderMix options:

    Gain and Focal Height Setting

    Directly specify the voltage to apply to the Photomultipler Tube (PMT) as Gain:
    FocalHeight can be set as a physical distance between the focal point to the lens:

    Gain and Focal Height Optimization

    Gain setting for the plate reader Photomultiplier Tube (PMT) can be optimized:
    Similarly FocalHeight can be optimized by providing a set of samples for the optimization:

Preferred Input Containers

    The CLARIOstar plate readers support 96- and 384-well plates for ExperimentAlphaScreen.

Data Processing

    Plot a boxplot of on two groups of AlphaScreen data objects:
    Plot a scatter plot on a group of AlphaScreen data objects and provided concentration information:

Warnings and Errors

    Messages  (51)

    AlphaScreenAssayPlateInfoRequired  (1)

    If a PreparedPlate is not used, plate and assay options should be specified. Otherwise, an error will be thrown:

    AlphaScreenConflictAliquotOption  (1)

    If Aliquot has a mix of True and False for SamplesIn in AlphaScreen, an error will be thrown:

    AlphaScreenCoverNotRecommended  (1)

    If a cover is retained on the plate, an error will be thrown:

    AlphaScreenFocalHeightDirectValueUnneeded  (1)

    If an OpticsOptimizationTemplate is given or focal height optimization is required, FocalHeight cannot specify to a numerical value. Otherwise, an error will be thrown:

    AlphaScreenFocalHeightOptimizationParametersRequired  (1)

    If a focal height optimization is required (OptimizeFocalHeight is True), relevant options should be specified. Otherwise, an error will be thrown:

    AlphaScreenFocalHeightOptimizationParametersUnneeded  (1)

    If a focal height optimization is not required (OptimizeFocalHeight is False), irrelevant options should not be specified. Otherwise, an error will be thrown:

    AlphaScreenGainDirectValueUnneeded  (2)

    If an OpticsOptimizationTemplate is given or gain optimization is required, Gain cannot specify to a numerical value. Otherwise, an error will be thrown:

    If an optics optimization template is given or gain optimization is required, gain is not set to a direct value. Otherwise, an error will be thrown:

    AlphaScreenGainOptimizationParametersRequired  (1)

    If a gain optimization is required (OptimizeGain is True), relevant options should be specified. Otherwise, an error will be thrown:

    AlphaScreenGainOptimizationParametersUnneeded  (1)

    If a gain optimization is not required (OptimizeGain is False), irrelevant options should not be specified. Otherwise, an error will be thrown:

    AlphaScreenHighWellVolume  (1)

    If the assay volume (AlphaAssayVolume) is above the recommended working volume of the plate, a warning will be thrown:

    AlphaScreenInvalidAssayPlate  (1)

    If the plate model is not compatible with the liquid handler, an error will be thrown:

    AlphaScreenInvalidEmissionWavelength  (1)

    If the emission wavelength is not set to 570nm, an error will be thrown:

    AlphaScreenInvalidExcitationWavelength  (1)

    If the excitation wavelength is not set to 680nm, an error will be thrown:

    AlphaScreenInvalidFocalHeightOptimizationSamples  (1)

    If focal height optimization is needed for a PreparedPlate, the focal height optimization samples should be members of SamplesIn. Otherwise, an error will be thrown:

    AlphaScreenInvalidGainOptimizationSamples  (1)

    If gain optimization is needed for a PreparedPlate, the gain optimization samples should be members of SamplesIn. Otherwise, an error will be thrown:

    AlphaScreenInvalidSampleAliquot  (1)

    Aliquot cannot be specified for a prepared plate, otherwise an error will be thrown:

    AlphaScreenInvalidSingleFocalHeightOptimizationSample  (1)

    If PreparedPlate->False, one focal height optimization sample should be given and aliquoted according to the NumberOfFocalHeightOptimization. Otherwise, an error will be thrown:

    AlphaScreenInvalidSingleGainOptimizationSample  (1)

    If PreparedPlate->False, one gain optimization sample should be given and aliquoted according to the NumberOfGainOptimization. Otherwise, an error will be thrown:

    AlphaScreenLowWellVolume  (1)

    If the assay volume (AlphaAssayVolume) is below the minimum working volume of the plate, a warning will be thrown:

    AlphaScreenMixParametersConflicting  (1)

    When plate reader mixing is resolved automatically, the relevant options must all be set to values or all be set to Null. Otherwise, an error will be thrown:

    AlphaScreenMixParametersRequired  (1)

    When plate reader mixing is needed (PlateReaderMix is True), the relevant options should be specified. Otherwise, an error will be thrown:

    AlphaScreenMixParametersUnneeded  (1)

    When plate reader mixing is not needed (PlateReaderMix is False), the irrelevant options should not be specified. Otherwise, an error will be thrown:

    AlphaScreenMoatAliquotsRequired  (1)

    Moats are created as part of sample aliquoting. As a result if a moat is requested Aliquot must then be set to True:

    AlphaScreenMoatParametersConflicting  (1)

    When moat is resolved automatically (MoatWells is Automatic), the relevant parameter options should be specified. Otherwise, an error will be thrown:

    AlphaScreenMoatParametersRequired  (1)

    When moat is needed (MoatWells is True), the relevant options should be specified. Otherwise, an error will be thrown:

    AlphaScreenMoatParametersUnneeded  (1)

    When moat is not needed (MoatWells is False), the irrelevant options should not be specified. Otherwise, an error will be thrown:

    AlphaScreenMoatVolumeOverflow  (1)

    The moat wells cannot be filled beyond the MaxVolume of the container:

    AlphaScreenMoatVolumeOverlimit  (1)

    The moat volumes cannot exceed the maximum volume limit of available plates:

    AlphaScreenNotBMGPlates  (2)

    A Warning is thrown if the plate mode doesn't match any available plate layouts in the reader:

    A Warning is thrown if the samples are in a prepared plate whose container doesn't match any available plate layouts in the reader:

    AlphaScreenNotSupportedPlateReader  (1)

    If the plate reader does not support AlphaScreen, an error will be thrown:

    AlphaScreenObjectNotExist  (1)

    If any of the objects in samples or options do not exist in database, an error will be thrown:

    AlphaScreenOpticsOptimizationParametersUnneeded  (1)

    If an optics optimization template is used, irrelevant options will be ignored with a warning:

    AlphaScreenPreparedPlateIrrelevant  (1)

    If a PreparedPlate is used, irrelevant options should not be specified. Otherwise, an error will be thrown:

    AlphaScreenRepeatedFocalHeightOptimizationSamples  (1)

    If PreparedPlate->True, the focal height optimization samples should not be repeated. Otherwise, an error will be thrown:

    AlphaScreenRepeatedGainOptimizationSamples  (1)

    If PreparedPlate->True, the gain optimization samples should not be repeated. Otherwise, an error will be thrown:

    AlphaScreenRepeatedPlateReaderSamples  (1)

    Throws an error if the same sample appears multiple time in the input list, but Aliquot has been set to False so aliquots of the repeated sample cannot be created for the read:

    AlphaScreenTooManyMoatWells  (1)

    Throws an error if moat size is above 3 which will limits the available wells for the input samples:

    AlphaScreenUnequalFocalHeightOptimizationSamples  (1)

    If PreparedPlate->True, the length of focal height optimization samples should match the NumberOfFocalHeightOptimizations. Otherwise, an error will be thrown:

    AlphaScreenUnequalGainOptimizationSamples  (1)

    If PreparedPlate->True, the length of gain optimization samples should match the NumberOfGainOptimizations. Otherwise, an error will be thrown:

    AlphaScreenWellOverlap  (1)

    The wells to be used for the moat cannot also be used to hold assay samples:

    AlphaScreenWellVolumeAboveLimit  (1)

    If the assay volume (AlphaAssayVolume) is above the highest working volume of any available plates, an error will be thrown:

    AlphaScreenWellVolumeBelowLimit  (1)

    If the assay volume (AlphaAssayVolume) is below the lowest working volume of any available plates, an error will be thrown:

    AlphaScreenWellVolumeExceeded  (1)

    If the assay volume (AlphaAssayVolume) exceeds the maximum working volume of the plate, an error will be thrown:

    DiscardedSamples  (1)

    If the provided sample is discarded, an error will be thrown:

    EmptyContainers  (1)

    All containers provided as input to the experiment must contain samples:

    FocalHeightDirectValueUnneeded  (1)

    If an optics optimization template is given or focal height optimization is required, focal height is not set to a direct value. Otherwise, an error will be thrown:

    InputContainsTemporalLinks  (1)

    A Warning is thrown if any inputs or options contain temporal links:

    NumberOptimizationSamples  (1)

    If not all optics optimization samples are in one plate, an error will be thrown:

    RetiredInstrument  (1)

    If the plate reader is retired, an error will be thrown:

Possible Issues

    Environment temperature

    AlphaScreen measurement is sensitive to temperature fluctuations. It is recommended to specify ReadEquilibrationTime so that the temperature of samples is stable before plate reader measurement.
Last modified on Sun 7 Apr 2024 00:39:40


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