Object[Protocol, CapillaryELISA]
A protocol for performing a capillary Enzyme-Linked Immunosorbent Assay (ELISA) experiment on the provided samples for the detection and quantification of certain analytes.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryELISA, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryELISA]
Pattern Description: Object[Protocol, CapillaryELISA]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The detection and quantification capillary ELISA device on which the protocol is run. The instrument accepts the cartridge loaded with samples and buffers, loads the samples into the capillaries, performs the ELISA experiment and detects the signals to quantify certain analytes.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[{Object[Instrument, CapillaryELISA], Model[Instrument, CapillaryELISA]}]
CartridgeType
The format of the capillary ELISA cartridge plate (SinglePlex72X1, MultiAnalyte32X4, MultiAnalyte16X4, MultiPlex32X8 or Customizable) used in this capillary ELISA protocol. The pre-loaded CartridgeType (SinglePlex72X1, MultiAnalyte16X4, MultiAnalyte32X4 and MultiPlex32X8) is pre-loaded with validated ELISA assay.
Format: Single
Class: Expression
Programmatic Pattern: ELISACartridgeTypeP
Cartridge
The capillary ELISA cartridge plate that is used in the instrument to perform ELISA experiments and quantify the analytes (such as peptides, proteins, antibodies and hormones) in the samples by ELISA.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[{Model[Container, Plate, Irregular, CapillaryELISA], Object[Container, Plate, Irregular, CapillaryELISA]}]
Species
The organism (human, mouse or rat) that the samples (containing analytes of interest) are derived from.
Format: Single
Class: Expression
Programmatic Pattern: ELISASpeciesP
Multiplex
Indicates if the protocol is a capillaryELISA protocol to detect and quantify multiple analytes in the same sample.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
AnalyteMolecules
For each member of SamplesIn, the identity model of the molecule (e.g., peptides, proteins, antibodies, hormones) detected and quantified in the samples through this capillary ELISA experiment.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Model[Molecule]]
Relation: Model[Molecule]
AnalyteNames
For each member of SamplesIn, the manufacturer's listed name(s) of the ELISA analyte(s) (e.g., peptides, proteins, antibodies, hormones) detected in the pre-loaded capillary ELISA cartridge assay.
Format: Multiple
Class: String
Programmatic Pattern: CapillaryELISAAnalyteP
MultiplexAnalyteMolecules
The multiplex analytes that are detected and quantified simultaneously in all the samples through this capillary ELISA experiment.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Model[Molecule]]
Relation: Model[Molecule]
MultiplexAnalyteNames
For each member of MultiplexAnalyteMolecules, the manufacturer's listed name of the analyte that us detected and quantified simultaneously in all the samples in a pre-loaded capillary ELISA cartridge through this capillary ELISA experiment.
Format: Multiple
Class: String
Programmatic Pattern: CapillaryELISAAnalyteP
ManufacturingSpecifications
The manufacturing specifications with the detailed information (including the PDF specification sheets) about the pre-loaded analytes in the pre-loaded capillary ELISA cartridge, provided by the manufacturer of the capillary ELISA cartridge.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Object[ManufacturingSpecification]]
Relation: Object[ManufacturingSpecification]
UpperQuantitationLimits
The average Upper Limit of Quantitation (ULOQ) concentrations of the analytes of the pre-loaded capillary ELISA cartridge. The values are determined by the assay developer through analysis of repeated ELISA experiments using multiple capillary ELISA cartridges. The concentrations of the analytes in the sample loaded into the capillary ELISA cartridge are expected to fall below ULOQ for the best quantifiaction result.
Format: Multiple
[[1]] Analyte
Header: Analyte
Class: String
[[2]] Upper Limit of Quantitation
Header: Upper Limit of Quantitation
Class: Real
Unit: 1 picogram per milliliter
LowerQuantitationLimits
The average Lower Limit of Quantitation (LLOQ) concentrations of the analytes of the pre-loaded capillary ELISA cartridge. The values are determined by the assay developer through analysis of repeated ELISA experiments using multiple capillary ELISA cartridges. The concentrations of the analytes in the sample loaded into the capillary ELISA cartridge are expected to fall above LLOQ for the best quantifiaction result.
Format: Multiple
[[1]] Analyte
Header: Analyte
Class: String
[[2]] Upper Limit of Quantitation
Header: Upper Limit of Quantitation
Class: Real
Unit: 1 picogram per milliliter
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
SampleVolumes
For each member of SamplesIn, the amount of the input sample that is aliquoted from its original container.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SpikeSamples
For each member of SamplesIn, the sample with known concentration(s) of analyte(s) mixed with the input sample to increase the concentration of the analyte(s) before any further dilution is performed.
Format: Multiple
Class: Link
SpikeVolumes
For each member of SamplesIn, the volume of the SpikeSample mixed with the input sample before further dilution is performed.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SerialDilution
For each member of SamplesIn, indicates whether the dilution used for the sample is the stepwise dilution or not. To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container and additional diluent is added to dilute the original solution. The diluted sample is then used as the base solution to make an additional dilution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
DilutionAssayVolumes
For each member of SamplesIn, the total preparation volume of each diluted sample in the non-serial dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StartingDilutionFactors
For each member of SamplesIn, the dilution factor of the first diluted sample compared to the original input sample in the non-serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
DilutionFactorIncrements
For each member of SamplesIn, the constant dilution factor increase of the diluted samples in the non-serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
NumberOfDilutions
For each member of SamplesIn, the number of diluted samples prepared in the non-serial dilution series. The dilution factor difference between the two adjacent samples is presented by DilutionFactorIncrements.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
DilutionCurves
For each member of SamplesIn, the collection of dilutions performed on the sample (after spiking) before loaded into capillary ELISA cartridge. This is the volume of the sample and the volume of the corresponding Diluents that is mixed together for each concentration in the dilution curve.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
SerialDilutionAssayVolumes
For each member of SamplesIn, the total preparation volume of each diluted sample in the serial dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SerialDilutionFactors
For each member of SamplesIn, the constant stepwise dilution factor of the diluted samples in the serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
NumberOfSerialDilutions
For each member of SamplesIn, the number of diluted samples prepared in the serial dilution series.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
SerialDilutionCurves
For each member of SamplesIn, the collection of serial dilutions performed on the sample (after spiking) before loaded into capillary ELISA cartridge. This is the volume taken out of the sample and transferred serially and the volume of the corresponding Diluents that is mixed with at each step.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
Diluents
For each member of SamplesIn, the buffer that is used to dilute the input sample (after spiking, if applicable).
Format: Multiple
Class: Link
DilutionMixVolumes
For each member of SamplesIn, the volume that is pipetted up and down to mix the sample with the corresponding Diluents to make the dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DilutionNumberOfMixes
For each member of SamplesIn, the number of pipette up and down cycles that is used to mix the sample with the corresponding Diluents to make the dilution series.
Format: Multiple
Class: Integer
Programmatic Pattern: RangeP[0, 20, 1]
DilutionMixRates
For each member of SamplesIn, the speed at which the DilutionMixVolumes is pipetted up and down to mix the sample with the corresponding Diluents to make the dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter per second
Programmatic Pattern: Microliter Microliter
Hold[RangeP[0.4 ----------, 250 ----------]]
Second Second
SamplePreparationPrimitives
A set of instructions specifying the preparation of antibody samples and standard samples for capillary ELISA experiments, including the resuspension and conjugation of the antibody samples and the resuspension of the standard samples.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
SamplePreparationProtocol
The sample manipulation protocol used to prepare antibody samples and standard samples for capillary ELISA experiments.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
SampleManipulationPrimitives
A set of instructions specifying the spiking of input samples, dilution of the input samples and standard samples, dilution of antibody samples for customizable cartridge and loading of all reagents into the capillary ELISA cartridge for ELISA experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
SampleManipulationProtocol
The sample manipulation protocol used to spike input samples, dilute samples and load samples into the capillary ELISA cartridge for ELISA experiment.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
StandardData
For each member of Standards, the ELISA data generated by this capillary ELISA protocol.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Object[Data]]
Relation: Object[Data][Protocol]
StandardCurveFunctions
The standard curve functions of the pre-loaded analytes in this ELISA experiment, provided by the assay developer. The function takes Concentration in Picogram/Milliliter as input and generates FluorescenceSignal in RFU as output. This standard curve is used to calculate the concentration(s) of this analyte in unknown sample(s) from the measured fluorescence signal(s).
Format: Multiple
[[1]] Analyte
Header: Analyte
Class: String
Programmatic Pattern: _String
[[2]] Standard Curve Function
Header: Standard Curve Function
Class: Expression
Programmatic Pattern: _QuantityFunction
MethodFile
The CYDAT file containing the run information and the run results generated by the capillary ELISA instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
DataFile
The CSV file containing the experiment data (concentration, %CV) generated by the capillary ELISA instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SpikeSampleStorageConditions
For each member of SamplesIn, the storage conditions under which the unused portion of the SpikeSample should be stored after their usage in the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Capture Antibody Preparation
CustomCaptureAntibodies
For each member of SamplesIn, the capture antibody samples used in this protocol to bind with analytes in the input samples through specific antigen-antibody interaction and capture the analytes into capillaries for detection. The capture antibody samples are resuspended (if in solid state), bioconjugated with digoxigenin and diluted before loaded into the customizable capillary ELISA cartridge.
Format: Multiple
Class: Link
CaptureAntibodyResuspensions
For each member of SamplesIn, indicates if resuspension is required to be performed on the solid state CustomCaptureAntibodies sample to turn the sample into solution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
CaptureAntibodyResuspension Concentrations
For each member of SamplesIn, the target concentration of the CustomCaptureAntibodies sample in the solution after resuspension.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
CaptureAntibodyResuspension Diluents
For each member of SamplesIn, the resuspension buffer mixed with the solid state CustomCaptureAntibodies sample to dissolve the sample into solution.
Format: Multiple
Class: Link
CaptureAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of resuspended CustomCaptureAntibodies sample inside its original container is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CaptureAntibodyConjugations
For each member of SamplesIn, indicates if bioconjugation reaction between digoxigenin NHS-ester and primary amines of the capture antibody is required to be performed to prepare digoxigenin-labeled capture antibody sample for capillary ELISA experiment.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
CaptureAntibodyVolumes
For each member of SamplesIn, the volume of the unconjugated capture antibody sample used to react with DigoxigeninReagents to prepare digoxigenin-labeled capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DigoxigeninReagents
For each member of SamplesIn, the digoxigenin NHS-ester reagent used to react with the primary amines of the unconjugated CustomCaptureAntibodies sample to prepare digoxigenin-labeled capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
DigoxigeninReagentVolumes
For each member of SamplesIn, the volume of DigoxigeninReagents used to react with CaptureAntibodyVolumes of the unconjugated CustomCaptureAntibodies sample to prepare digoxigenin-labeled capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CaptureAntibodyConjugation Buffers
For each member of SamplesIn, the buffer solution in which the reaction between the unconjugated CustomCaptureAntibodies sample and DigoxigeninReagent happens to prepare digoxigenin-labeled capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
CaptureAntibodyConjugation BufferVolumes
For each member of SamplesIn, the volume of CaptureAntibodyConjugationBuffers used to mix with the unconjugated CustomCaptureAntibodies and DigoxigeninReagents to provide a buffered environment for the bioconjugation reaction.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CaptureAntibodyConjugation Containers
For each member of SamplesIn, the container in which the unconjugated CustomCaptureAntibodies sample, DigoxigeninReagents and CaptureAntibodyConjugationBuffers react to allow bioconjugation synthesis.
Format: Multiple
Class: Link
CaptureAntibodyConjugation Times
For each member of SamplesIn, the amount of reaction time that the CustomCaptureAntibodies and DigoxigeninReagents are allowed to react before purification happens.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
CaptureAntibodyConjugation Temperatures
For each member of SamplesIn, the temperature at which the reaction between the unconjugated CustomCaptureAntibodies and DigoxigeninReagents is conducted.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
CaptureAntibodyPurification Columns
For each member of SamplesIn, the desalting spin column used to adsorb the molecules that are smaller than its molecular weight cut-off (MWCO) on its resin bed to remove the free digoxigenin NHS-ester molecules in order to purify the synthesis product of the capture antibody bioconjugation reaction.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[{Model[Container, Vessel, Filter], Object[Container, Vessel, Filter]}]
CaptureAntibodyColumnWash Buffers
For each member of SamplesIn, the buffer solution loaded into the CaptureAntibodyPurificationColumns after its storage buffer is removed and before the sample is loaded.
Format: Multiple
Class: Link
CaptureAntibodyConjugation StorageConditions
For each member of SamplesIn, indicates the condition under which the unused portion of the synthesized and purified digoxigenin-labeled capture antibody sample is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CaptureAntibodyDilutions
For each member of SamplesIn, indicates if dilution is required to be performed on the digoxigenin-labeled capture antibody sample with the specified CaptureAntibodyDiluents to make a sample with desired concentration to be loaded into the customizable cartridge for ELISA experiment of the input sample.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
CaptureAntibodyTarget Concentrations
For each member of SamplesIn, the desired concentration of the digoxigenin-labeled capture antibody after dilution.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
CaptureAntibodyDiluents
For each member of SamplesIn, the buffer solution used to mix with the concentrated digoxigenin-labeled capture antibody sample to achieve the desired CaptureAntibodyTargetConcentrations for cartridge loading.
Format: Multiple
Class: Link
Detection Antibody Preparation
CustomDetectionAntibodies
For each member of SamplesIn, the detection antibody sample used in the sandwich ELISA experiment to incubate with analytes through specific antigen-antibody interaction after their binding with capture antibody to provide detection attachment sites for streptavidin-conjugated fluorescent dye. The detection antibody samples are resuspended (if in solid state), bioconjugated with biotin and diluted before loaded into the customizable capillary ELISA cartridge.
Format: Multiple
Class: Link
DetectionAntibody Resuspensions
For each member of SamplesIn, indicates if resuspension is required to be performed on the solid state CustomDetectionAntibodies sample to turn the sample into solution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
DetectionAntibodyResuspension Concentrations
For each member of SamplesIn, the target concentration of the CustomDetectionAntibodies sample in the solution after resuspension.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
DetectionAntibodyResuspension Diluents
For each member of SamplesIn, the resuspension buffer mixed with the solid state CustomDetectionAntibodies sample to dissolve the sample into solution.
Format: Multiple
Class: Link
DetectionAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of resuspended CustomDetectionAntibodies sample inside its original container is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
DetectionAntibodyConjugations
For each member of SamplesIn, indicates if bioconjugation reaction between Biotin-XX-SE (6-((6-((Biotinoyl)Amino)Hexanoyl)Amino)Hexanoic Acid, Succinimidyl Ester) and primary amines of the CustomDetectionAntibody is required to be performed to prepare biotinylated detection antibody sample for capillary ELISA experiment.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
DetectionAntibodyVolumes
For each member of SamplesIn, the volume of the unconjugated detection antibody sample used to react with BiotinReagents to prepare biotinylated detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BiotinReagents
For each member of SamplesIn, the digoxigenin NHS-ester reagent used to react with the primary amines of the unconjugated CustomDetectionAntibodies sample to prepare biotinylated detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
BiotinReagentVolumes
For each member of SamplesIn, the volume of BiotinReagents used to react with DetectionAntibodyVolumes of the unconjugated CustomDetectionAntibodies sample to prepare biotinylated detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DetectionAntibodyConjugation Buffers
For each member of SamplesIn, the buffer solution in which the reaction between the unconjugated CustomDetectionAntibodies sample and BiotinReagents happens to prepare biotinylated detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
DetectionAntibodyConjugation BufferVolumes
For each member of SamplesIn, the volume of DetectionAntibodyConjugationBuffers used to mix with the unconjugated CustomDetectionAntibodies and BiotinReagents to provide a buffered environment for the bioconjugation reaction.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DetectionAntibodyConjugation Containers
For each member of SamplesIn, the container in which the unconjugated CustomDetectionAntibodies sample, BiotinReagents and DetectionAntibodyConjugationBuffers react to allow bioconjugation synthesis.
Format: Multiple
Class: Link
DetectionAntibodyConjugation Times
For each member of SamplesIn, the amount of reaction time that the CustomDetectionAntibodies and BiotinReagents are allowed to react before purification happens.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
DetectionAntibodyConjugation Temperatures
For each member of SamplesIn, the temperature at which the reaction between the unconjugated CustomDetectionAntibodies and BiotinReagents is conducted.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
DetectionAntibodyPurification Columns
For each member of SamplesIn, the desalting spin column used to adsorb the molecules that are smaller than its molecular weight cut-off (MWCO) on its resin bed to remove the free biotinylation reagent molecules in order to purify the synthesis product of the detection antibody bioconjugation reaction.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[{Model[Container, Vessel, Filter], Object[Container, Vessel, Filter]}]
DetectionAntibodyColumnWash Buffers
For each member of SamplesIn, the buffer solution loaded into the DetectionAntibodyPurificationColumns after its storage buffer is removed and before the sample is loaded.
Format: Multiple
Class: Link
DetectionAntibodyConjugation StorageConditions
For each member of SamplesIn, indicates the condition under which the unused portion of the synthesized and purified biotinylated detection antibody sample is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
DetectionAntibodyDilutions
For each member of SamplesIn, indicates if dilution is required to be performed on the biotinylated detection antibody sample with the specified DetectionAntibodyDiluents to make a sample with desired concentration to be loaded into the customizable cartridge for ELISA experiment of the input sample.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
DetectionAntibodyTarget Concentrations
For each member of SamplesIn, the desired concentration of the biotinylated detection antibody after dilution.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
DetectionAntibodyDiluents
For each member of SamplesIn, the buffer solution used to mix with the concentrated biotinylated detection antibody sample to achieve the desired DetectionAntibodyTargetConcentrations for cartridge loading.
Format: Multiple
Class: Link
Standard Preparation
Standards
The standard samples with known concentration(s) of analyte(s) run with the input unknown samples in the same capillary ELISA experiment. The standard samples are resuspended (if in solid state) and diluted before loaded into the capillary ELISA cartridge.
Format: Multiple
Class: Link
StandardResuspensions
For each member of Standards, indicates if resuspension is required to be performed on the solid state Standards sample to turn the sample into solution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardResuspension Concentrations
For each member of Standards, the target concentration of the Standards sample in the solution after resuspension.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
StandardResuspensionDiluents
For each member of Standards, the resuspension buffer mixed with the solid state Standards sample to dissolve the sample into solution.
Format: Multiple
Class: Link
StandardStorageConditions
For each member of Standards, the condition under which the unused portion of the resuspended Standards inside its original container is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardSerialDilution
For each member of Standards, indicates whether the dilution used for the standard sample is the stepwise dilution or not. To perform a serial dilution, a small amount of a well-mixed solution is transferred into a new container and additional diluent is added to dilute the original solution. The diluted sample is then used as the base solution to make an additional dilution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardDilutionAssayVolumes
For each member of Standards, the total preparation volume of each diluted sample in the non-serial dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardStartingDilution Factors
For each member of Standards, the dilution factor of the first diluted sample compared to the original standard sample in the non-serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardDilutionFactor Increments
For each member of Standards, the constant dilution factor increase of the diluted samples in the non-serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardNumberOfDilutions
For each member of Standards, the number of diluted samples prepared in the non-serial dilution series. The dilution factor difference between the two adjacent samples is presented by StandardDilutionFactorIncrements.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
StandardDilutionCurves
For each member of Standards, the collection of dilutions performed on the standard sample before loaded into capillary ELISA cartridge. This is the volume of the sample and the volume of the corresponding Diluents that is mixed together for each concentration in the dilution curve.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
StandardSerialDilutionAssay Volumes
For each member of Standards, the total preparation volume of each diluted sample in the serial dilution series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardSerialDilutionFactors
For each member of Standards, the constant stepwise dilution factor of the diluted samples in the serial dilution series.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardNumberOfSerial Dilutions
For each member of Standards, the number of diluted samples prepared in the serial dilution series.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
StandardSerialDilutionCurves
For each member of Standards, the collection of serial dilutions performed on the standard sample before loaded into capillary ELISA cartridge. This is the volume taken out of the sample and transferred serially and the volume of the corresponding Diluents that is mixed with at each step.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
StandardDiluents
For each member of Standards, the diluent buffer that is used to mix with the standard sample to make the concentration series.
Format: Multiple
Class: Link
StandardDilutionMixVolumes
For each member of Standards, the volume that is pipetted up and down to mix the standard sample with the corresponding StandardDiluents to make the concentration series.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDilutionNumberOfMixes
For each member of Standards, the number of pipette up and down cycles that is used to mix the standard sample with the corresponding StandardDiluents to make the concentration series.
Format: Multiple
Class: Integer
Programmatic Pattern: RangeP[0, 20, 1]
StandardDilutionMixRates
For each member of Standards, the speed at which the DilutionMixVolume is pipetted up and down to mix the standard sample with the corresponding StandardDiluents to make the concentration series.
Format: Multiple
Class: Real
Unit: 1 microliter per second
Programmatic Pattern: Microliter Microliter
Hold[RangeP[0.4 ----------, 250 ----------]]
Second Second
Standard Capture Antibody Preparation
StandardCaptureAntibodies
For each member of Standards, the standard capture antibody samples used in this protocol to bind with analytes in the input samples through specific antigen-antibody interaction and capture the analytes into capillaries for detection. The standard capture antibody samples are resuspended (if in solid state), bioconjugated with digoxigenin and diluted before loaded into the Standardizable capillary ELISA cartridge.
Format: Multiple
Class: Link
StandardCaptureAntibody Resuspensions
For each member of Standards, indicates if resuspension is required to be performed on the solid state StandardCaptureAntibodies sample to turn the sample into solution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardCaptureAntibody ResuspensionConcentrations
For each member of Standards, the target concentration of the StandardCaptureAntibodies sample in the solution after resuspension.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
StandardCaptureAntibody ResuspensionDiluents
For each member of Standards, the resuspension buffer mixed with the solid state StandardCaptureAntibodies sample to dissolve the sample into solution.
Format: Multiple
Class: Link
StandardCaptureAntibody StorageConditions
For each member of Standards, the condition under which the unused portion of resuspended StandardCaptureAntibodies sample inside its original container is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardCaptureAntibody Conjugations
For each member of Standards, indicates if bioconjugation reaction between digoxigenin NHS-ester and primary amines of the standard capture antibody is required to be performed to prepare digoxigenin-labeled standard capture antibody sample for capillary ELISA experiment.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardCaptureAntibody Volumes
For each member of Standards, the volume of the unconjugated standard capture antibody sample used to react with DigoxigeninReagents to prepare digoxigenin-labeled standard capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDigoxigeninReagents
For each member of Standards, the digoxigenin NHS-ester reagent used to react with the primary amines of the unconjugated StandardCaptureAntibodies sample to prepare digoxigenin-labeled standard capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
StandardDigoxigeninReagent Volumes
For each member of Standards, the volume of DigoxigeninReagents used to react with StandardCaptureAntibodyVolumes of the unconjugated StandardCaptureAntibodies sample to prepare digoxigenin-labeled standard capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardCaptureAntibody ConjugationBuffers
For each member of Standards, the buffer solution in which the reaction between the unconjugated StandardCaptureAntibodies sample and DigoxigeninReagent happens to prepare digoxigenin-labeled standard capture antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
StandardCaptureAntibody ConjugationBufferVolumes
For each member of Standards, the volume of StandardCaptureAntibodyConjugationBuffers used to mix with the unconjugated StandardCaptureAntibodies and DigoxigeninReagents to provide a buffered environment for the bioconjugation reaction.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardCaptureAntibody ConjugationContainers
For each member of Standards, the container in which the unconjugated StandardCaptureAntibodies sample, DigoxigeninReagents and StandardCaptureAntibodyConjugationBuffers react to allow bioconjugation synthesis.
Format: Multiple
Class: Link
StandardCaptureAntibody ConjugationTimes
For each member of Standards, the amount of reaction time that the StandardCaptureAntibodies and DigoxigeninReagents are allowed to react before purification happens.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
StandardCaptureAntibody ConjugationTemperatures
For each member of Standards, the temperature at which the reaction between the unconjugated StandardCaptureAntibodies and DigoxigeninReagents is conducted.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardCaptureAntibody PurificationColumns
For each member of Standards, the desalting spin column used to adsorb the molecules that are smaller than its molecular weight cut-off (MWCO) on its resin bed to remove the free digoxigenin NHS-ester molecules in order to purify the synthesis product of the standard capture antibody bioconjugation reaction.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[{Model[Container, Vessel, Filter], Object[Container, Vessel, Filter]}]
StandardCaptureAntibodyColumn WashBuffers
For each member of Standards, the buffer solution loaded into the StandardCaptureAntibodyPurificationColumns after its storage buffer is removed and before the sample is loaded.
Format: Multiple
Class: Link
StandardCaptureAntibody ConjugationStorageConditions
For each member of Standards, indicates the condition under which the unused portion of the synthesized and purified digoxigenin-labeled standard capture antibody sample is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardCaptureAntibody Dilutions
For each member of Standards, indicates if dilution is required to be performed on the digoxigenin-labeled standard capture antibody sample with the specified StandardCaptureAntibodyDiluents to make a sample with desired concentration to be loaded into the Standardizable cartridge for ELISA experiment of the input sample.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardCaptureAntibodyTarget Concentrations
For each member of Standards, the desired concentration of the digoxigenin-labeled standard capture antibody after dilution.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
StandardCaptureAntibody Diluents
For each member of Standards, the buffer solution used to mix with the concentrated digoxigenin-labeled standard capture antibody sample to achieve the desired StandardCaptureAntibodyTargetConcentrations for cartridge loading.
Format: Multiple
Class: Link
Standard Detection Antibody Preparation
StandardDetectionAntibodies
For each member of Standards, the standard detection antibody sample used in the sandwich ELISA experiment to incubate with analytes through specific antigen-antibody interaction after their binding with standard capture antibody to provide detection attachment sites for streptavidin-conjugated fluorescent dye. The standard detection antibody samples are resuspended (if in solid state), bioconjugated with biotin and diluted before loaded into the Standardizable capillary ELISA cartridge.
Format: Multiple
Class: Link
StandardDetectionAntibody Resuspensions
For each member of Standards, indicates if resuspension is required to be performed on the solid state StandardDetectionAntibodies sample to turn the sample into solution.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardDetectionAntibody ResuspensionConcentrations
For each member of Standards, the target concentration of the StandardDetectionAntibodies sample in the solution after resuspension.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
StandardDetectionAntibody ResuspensionDiluents
For each member of Standards, the resuspension buffer mixed with the solid state StandardDetectionAntibodies sample to dissolve the sample into solution.
Format: Multiple
Class: Link
StandardDetectionAntibody StorageConditions
For each member of Standards, the condition under which the unused portion of resuspended StandardDetectionAntibodies sample inside its original container is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardDetectionAntibody Conjugations
For each member of Standards, indicates if bioconjugation reaction between Biotin-XX-SE (6-((6-((Biotinoyl)Amino)Hexanoyl)Amino)Hexanoic Acid, Succinimidyl Ester) and primary amines of the StandardDetectionAntibody is required to be performed to prepare biotinylated standard detection antibody sample for capillary ELISA experiment.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardDetectionAntibody Volumes
For each member of Standards, the volume of the unconjugated standard detection antibody sample used to react with BiotinReagents to prepare biotinylated standard detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardBiotinReagents
For each member of Standards, the digoxigenin NHS-ester reagent used to react with the primary amines of the unconjugated StandardDetectionAntibodies sample to prepare biotinylated standard detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
StandardBiotinReagentVolumes
For each member of Standards, the volume of BiotinReagents used to react with StandardDetectionAntibodyVolumes of the unconjugated StandardDetectionAntibodies sample to prepare biotinylated standard detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDetectionAntibody ConjugationBuffers
For each member of Standards, the buffer solution in which the reaction between the unconjugated StandardDetectionAntibodies sample and BiotinReagents happens to prepare biotinylated standard detection antibody sample through bioconjugation synthesis.
Format: Multiple
Class: Link
StandardDetectionAntibody ConjugationBufferVolumes
For each member of Standards, the volume of StandardDetectionAntibodyConjugationBuffers used to mix with the unconjugated StandardDetectionAntibodies and BiotinReagents to provide a buffered environment for the bioconjugation reaction.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDetectionAntibody ConjugationContainers
For each member of Standards, the container in which the unconjugated StandardDetectionAntibodies sample, BiotinReagents and StandardDetectionAntibodyConjugationBuffers react to allow bioconjugation synthesis.
Format: Multiple
Class: Link
StandardDetectionAntibody ConjugationTimes
For each member of Standards, the amount of reaction time that the StandardDetectionAntibodies and BiotinReagents are allowed to react before purification happens.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
StandardDetectionAntibody ConjugationTemperatures
For each member of Standards, the temperature at which the reaction between the unconjugated StandardDetectionAntibodies and BiotinReagents is conducted.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardDetectionAntibody PurificationColumns
For each member of Standards, the desalting spin column used to adsorb the molecules that are smaller than its molecular weight cut-off (MWCO) on its resin bed to remove the free biotinylation reagent molecules in order to purify the synthesis product of the standard detection antibody bioconjugation reaction.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[{Model[Container, Vessel, Filter], Object[Container, Vessel, Filter]}]
StandardDetectionAntibody ColumnWashBuffers
For each member of Standards, the buffer solution loaded into the StandardDetectionAntibodyPurificationColumns after its storage buffer is removed and before the sample is loaded.
Format: Multiple
Class: Link
StandardDetectionAntibody ConjugationStorageConditions
For each member of Standards, indicates the condition under which the unused portion of the synthesized and purified biotinylated standard detection antibody sample is stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardDetectionAntibody Dilutions
For each member of Standards, indicates if dilution is required to be performed on the biotinylated standard detection antibody sample with the specified StandardDetectionAntibodyDiluents to make a sample with desired concentration to be loaded into the Standardizable cartridge for ELISA experiment of the input sample.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardDetectionAntibody TargetConcentrations
For each member of Standards, the desired concentration of the biotinylated standard detection antibody after dilution.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Gram
Hold[GreaterP[0 Molar] | GreaterP[0 -----]]
Liter
StandardDetectionAntibody Diluents
For each member of Standards, the buffer solution used to mix with the concentrated biotinylated standard detection antibody sample to achieve the desired StandardDetectionAntibodyTargetConcentrations for cartridge loading.
Format: Multiple
Class: Link
Washing
WashBuffer
The buffer that is loaded in the capillary ELISA cartridge to automatically flow through the capillaries to remove excess reagents between the antibody binding steps of analyte(s) with capture antibody and detection antibody.
Format: Single
Class: Link
Cartridge Loading
LoadingVolumes
For each member of SamplesIn, the amount of each diluted sample (with SpikeSample mixed) that is loaded into the capillary ELISA cartridge plate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardLoadingVolumes
For each member of Standards, the volume of each diluted standard sample loaded into the capillary ELISA cartridge for capillary ELISA experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CaptureAntibodyLoadingVolumes
For each member of SamplesIn, the volume of the capture antibody sample that is loaded with the dilutions of this input sample into the capillary ELISA cartridge for ELISA assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DetectionAntibodyLoading Volumes
For each member of SamplesIn, the volume of the detection antibody sample that is loaded with the dilutions of this input sample into the capillary ELISA cartridge for ELISA assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardCaptureAntibody LoadingVolumes
For each member of Standards, the volume of the capture antibody sample that is loaded with the dilutions of this input sample into the capillary ELISA cartridge for ELISA assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDetectionAntibody LoadingVolumes
For each member of Standards, the volume of the detection antibody sample that is loaded with the dilutions of this input sample into the capillary ELISA cartridge for ELISA assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
Data Processing
StandardCompositions
For each member of Standards, the known concentration(s) of the analyte(s) in each standard sample before dilution.
Format: Multiple
Class: Expression
Programmatic Pattern: Picogram
Hold[{{GreaterEqualP[0 ----------] | GreaterEqualP[0 Molar], ObjectP[Model[Molecule]]}..}]
Milliliter
Relation: ['Null', 'Model[Molecule]']
SpikeConcentrations
For each member of SamplesIn, the known concentration(s) of analyte(s) in the spike standard sample mixed with the input sample before any dilution.
Format: Multiple
Class: Expression
Programmatic Pattern: Picogram
Hold[{{GreaterEqualP[0 ----------] | GreaterEqualP[0 Molar], ObjectP[Model[Molecule]]}..}]
Milliliter
Relation: ['Null', 'Model[Molecule]']
Last modified on Mon 26 Sep 2022 15:43:49