Object[Protocol, CapillaryIsoelectricFocusing]
A protocol for performing a capillary IsoElectric Focusing (cIEF) experiment on samples for electrophoretic separation over a linear pH gradient.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryIsoelectricFocusing, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, CapillaryIsoelectricFocusing]
Pattern Description: Object[Protocol, CapillaryIsoelectricFocusing]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The device on which the protocol is run. The instrument is loaded with a cartridge that holds the capillary in which cIEF is performed as well as electrolyte solutions required for this experiment, as well as additional buffers. Protein samples mixed with ampholytes and other components are loaded, one by one, on to the capillary by vacuum and a voltage is applied. As a result, analytes migrate in the in the formed pH gradient to their isoelectric point and are detected by UV absorption and native fluorescence.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[{Model[Instrument, ProteinCapillaryElectrophoresis], Object[Instrument, ProteinCapillaryElectrophoresis]}]
RunTime
The total time capillary isoelectric focusing is expected to run.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Second]
InjectionTable
The sequence of injections for a given experiment run for SamplesIn, Standards, and Blanks.
Format: Multiple
[Type]
Header: Type
Class: Expression
[Sample]
Header: Sample
Class: Link
[Volume]
Header: Volume
Class: Real
Unit: 1 microliter
[Data]
Header: Data
Class: Link
[SampleIndex]
Header: SampleIndex
Class: Integer
MethodFile
The methods file containing the run parameters for all samples in protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InjectionListFile
The InjectionList file containing the sample order and associated method name for all samples in the protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
SamplePreparationPrimitives
A set of instructions specifying the preparation of samples, standards, and blanks for capillary Isoelectric Focusing experiments, including addition of ampholytes, ElectroosmoticFlowBlocker, Denaturant, and pI marker.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
OnBoardMixingContainers Primitives
A set of instructions specifying the transfer of master mix from preparation tube to on board mixing vials.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
CartridgeCleanupPrimitives
A set of instructions specifying the transfer of buffers out of the experiment cartridge.
Format: Multiple
Class: Expression
Programmatic Pattern: ManualSamplePreparationP
CartridgePreparation Primitives
A set of instructions specifying the transfer of the anolyte and catholyte to the CIEF cartridge.
Format: Multiple
Class: Expression
Programmatic Pattern: ManualSamplePreparationP
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AssayContainer
The containers the samples are assayed in.
Format: Single
Class: Link
Programmatic Pattern: _Link
CartridgeCleanupWaste Container
Indicates the container used for waste in cleanup.
Format: Single
Class: Link
Programmatic Pattern: _Link
OnBoardMixing
Indicates if samples should be mixed onboard or on a liquid handler. When using OnBoardMixing, Sample tubes should contain samples in 25 microliters. Before injecting each sample, the instrument will add and mix 100 microliters of MasterMix.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
OnBoardMixingContainers
Indicates the containers (6 ml glass vials) used for holding the master mix for onboard mixing.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
OnBoardMixingPrepContainer
Indicates the container (50 ml tube, for liquid handler compatibility) used for preparing the master mix for onboard mixing.
Format: Single
Class: Link
Programmatic Pattern: _Link
OnBoardMixingWash
Indicates the water containers (6 ml glass vials) used for washing the capillary in onboard mixing.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
TotalVolumes
For each member of SamplesIn, TotalVolumes indicates the final volume in the assay tube prior to loading onto the capillary. Each tube contains a Sample, DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SampleVolumes
For each member of SamplesIn, indicates the volume of sample required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PremadeMasterMixReagents
For each member of SamplesIn, indicates the premade master mix used. The master mix contains the reagents required for cIEF experiments, i.e., DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PremadeMasterMixDiluents
For each member of SamplesIn, indicates the premade master mix diluent used. The diluent solution dilutes the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PremadeMasterMixReagent DilutionFactors
For each member of SamplesIn, indicates the factor by which the premade master mix needs to be diluted by.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
PremadeMasterMixVolumes
For each member of SamplesIn, indicates the volume of the premade master mix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
Diluents
For each member of SamplesIn, indicates the diluent buffer, e.g., Milli-Q water, added to the master mix to dilute all components to working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DenaturationReagents
For each member of SamplesIn, indicates the denaturing agent, e.g., Urea or SimpleSol, added to the master mix to prevent protein precipitation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DenaturationReagentTarget Concentrations
For each member of SamplesIn, indicates the final concentration of the denaturing agent in the mastermix.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
DenaturationReagentVolumes
For each member of SamplesIn, indicates the volumes of the denaturing agent required to reach its final concentration in the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
Ampholytes
For each member of SamplesIn, indicates the makeup of amphoteric molecules in the master mix that form the pH gradient.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
AmpholyteTargetConcentrations
For each member of SamplesIn, indicates the concentrations (Vol/Vol) of amphoteric molecules in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
AmpholyteVolumes
For each member of SamplesIn, indicates the volumes of amphoteric molecule stocks to add to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
IsoelectricPointMarkers
For each member of SamplesIn, indicates Reference analytes included in the mastermix. pI markers facilitate the interpolation of sample pI. The pH is then interpolated by the straight line between the pI markers.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
IsoelectricPointMarkersTarget Concentrations
For each member of SamplesIn, indicates the final concentrations (Vol/Vol) of pI markers in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
IsoelectricPointMarkers Volumes
For each member of SamplesIn, indicates the volume of pI marker stocks added to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
AnodicSpacers
For each member of SamplesIn, indicates the Acidic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
AnodicSpacerTarget Concentrations
For each member of SamplesIn, indicates the final concentrations of AnodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
AnodicSpacerVolumes
For each member of SamplesIn, indicates the volume of AnodicSpacer stock added to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CathodicSpacers
For each member of SamplesIn, indicates the basic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
CathodicSpacerTarget Concentrations
For each member of SamplesIn, indicates the final concentrations of CathodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
CathodicSpacerVolumes
For each member of SamplesIn, indicates the volume of CathodicSpacer stock added to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ElectroosmoticFlowBlockers
For each member of SamplesIn, indicates the solution added to samples to minimize electroOsmotic flow in the capillary.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ElectroosmoticFlowBlocker TargetConcentrations
For each member of SamplesIn, indicates the final concentrations of ElectroosmoticFlowBlockers in the mastermix.
Format: Multiple
Class: Real
Unit: MassPercent
Programmatic Pattern: RangeP[0 MassPercent, 100 MassPercent]
ElectroosmoticFlowBlocker Volumes
For each member of SamplesIn, indicates the volumes of ElectroosmoticFlowBlockers stock to add to the master mix for each sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SamplePreparationProtocol
The sample manipulation protocol used to prepare samples for capillary Isoelectric Focusing experiments.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
DataFile
The output data file generated at the conclusion of the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
AnolyteStorageCondition
The non-default storage condition for the anolyte solution once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
CatholyteStorageCondition
The non-default storage condition for the catholyte solution once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
ElectroosmoticConditioning BufferStorageCondition
The non-default storage condition for the ElectroosmoticConditioningBuffer once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
FluorescenceCalibration StandardStorageCondition
The non-default storage condition for the FluorescenceCalibrationStandard once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
WashSolutionStorageCondition
The non-default storage condition for the WashSolution once the experiment is set up.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
AmpholytesStorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the Ampholyte solutions after sample is prepared.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
IsoelectricPointMarkers StorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the IsoelectricPointMarkers after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
DenaturationReagentStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the Denaturation Reagent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
AnodicSpacerStorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the Anodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
CathodicSpacerStorage Conditions
For each member of SamplesIn, indicates the non-default storage condition for the cathodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
ElectroosmoticFlowBlocker StorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the ElectroosmoticFlowBlocker after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
DiluentStorageConditions
For each member of SamplesIn, indicates the non-default storage condition for the Diluent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
StandardStorageConditions
For each member of Standards, indicates the non-default storage condition.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardAmpholytesStorage Conditions
For each member of Standards, indicates the non-default storage condition for the Ampholyte solutions after sample is prepared.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
StandardIsoelectricPoint MarkersStorageConditions
For each member of Standards, indicates the non-default storage condition for the IsoelectricPointMarkers after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
StandardDenaturationReagent StorageConditions
For each member of Standards, indicates the non-default storage condition for the Denaturation Reagent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
StandardAnodicSpacerStorage Conditions
For each member of Standards, indicates the non-default storage condition for the Anodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
StandardCathodicSpacerStorage Conditions
For each member of Standards, indicates the non-default storage condition for the cathodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
StandardElectroosmoticFlow BlockerStorageConditions
For each member of Standards, indicates the non-default storage condition for the ElectroosmoticFlowBlocker after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
StandardDiluentStorage Conditions
For each member of Standards, indicates the non-default storage condition for the StandardDiluent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
BlankStorageConditions
For each member of Blanks, indicates the non-default storage condition.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankAmpholytesStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the Ampholyte solutions after sample is prepared.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
BlankIsoelectricPointMarkers StorageConditions
For each member of Blanks, indicates the non-default storage condition for the IsoelectricPointMarkers after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: {(SampleStorageTypeP | Disposal | Null)..}
BlankDenaturationReagent StorageConditions
For each member of Blanks, indicates the non-default storage condition for the Denaturation Reagent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
BlankAnodicSpacerStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the Anodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
BlankCathodicSpacerStorage Conditions
For each member of Blanks, indicates the non-default storage condition for the cathodic Spacer after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
BlankElectroosmoticFlow BlockerStorageConditions
For each member of Blanks, indicates the non-default storage condition for the ElectroosmoticFlowBlocker after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
BlankDiluentStorageConditions
For each member of Blanks, indicates the non-default storage condition for the BlankDiluent after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
CartridgeStorageCondition
The non-default storage condition for the Cartridge after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal | Null
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Instrument Setup
Cartridge
The capillary electrophoresis cartridge loaded on the instrument for Capillary IsoElectric Focusing (cIEF) experiments. The cartridge holds a single capillary and electrolyte buffers (sources of hydronium and hydroxyl ions).
Format: Single
Class: Link
Programmatic Pattern: _Link
SampleTemperature
The sample tray temperature at which samples are maintained while awaiting injection.
Format: Single
Class: Integer
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
Caps
The caps used to seal reagent vials during an isoelectric focusing experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersWithPressureCaps
Containers that are currently capped with pressure caps.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
ContainersWithClearCaps
Containers that are currently capped with clear caps.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
TransferPipette
The disposable transfer pipette used to clean the cartridge ports before storage.
Format: Single
Class: Link
Programmatic Pattern: _Link
Anolyte
The electrolyte solution loaded on the cartridge that is the source of hydronium ions for the capillary in cIEF experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
Catholyte
The electrolyte solution loaded on the cartridge that is the source of hydroxyl ions for the capillary in cIEF experiments.
Format: Single
Class: Link
Programmatic Pattern: _Link
ElectroosmoticConditioning Buffer
The ElectroosmoticConditioningBuffer solution is used to wash the capillary between injections.
Format: Single
Class: Link
Programmatic Pattern: _Link
FluorescenceCalibration Standard
The FluorescenceCalibrationStandard solution is used to adjust the baseline and normalize the signal for detection.
Format: Single
Class: Link
Programmatic Pattern: _Link
WashSolution
The solution used to rinse the capillary after use.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PlaceholderContainer
An empty vial used to dry the capillary after wash.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PlateSeal
The adhesive film used to seal the assay plate once it is prepared for injection. The seal should be pierceable, if not, it should be removed before loading the plate to the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Separation
LoadTimes
For each member of SamplesIn, indicates the time to load samples mixed with master mix into the capillary by vacuum.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterP[0 Second]
VoltageDurationProfiles
For each member of SamplesIn, indicates the series of voltages and durations to apply onto the capillary for separation.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
StandardLoadTimes
For each member of Standards, indicates the time to load samples mixed with master mix into the capillary by vacuum.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterP[0 Second]
Imaging
ImagingMethods
For each member of SamplesIn, indicates the whole capillary imaging methods for each sample.
Format: Multiple
Class: Expression
Programmatic Pattern: Absorbance | AbsorbanceAndFluorescence
NativeFluorescenceExposure Times
For each member of SamplesIn, indicates exposure durations for NativeFluorescence detection.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterP[0 Second]..}
Standards
Standards
Known analytes that serve as standards for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardTotalVolumes
For each member of Standards, TotalVolumes indicates the final volume in the assay tube prior to loading onto the capillary. Each tube contains a Sample, DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardVolumes
For each member of Standards, indicates the volume of blank required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardPremadeMasterMix Reagents
For each member of Standards, indicates the premade master mix used. The master mix contains the reagents required for cIEF experiments, i.e., DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardPremadeMasterMix Diluents
For each member of Standards, indicates the premade master mix diluent used. The diluent solution dilutes the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardPremadeMasterMix ReagentDilutionFactors
For each member of Standards, indicates the factor by which the premade master mix needs to be diluted by.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
StandardPremadeMasterMix Volumes
For each member of Standards, indicates the volume of the premade master mix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardDiluents
For each member of Standards, indicates the diluent buffer, e.g., Milli-Q water, added to the master mix to dilute all components to working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardDenaturationReagents
For each member of Standards, indicates the denaturing agent, e.g., Urea or SimpleSol, added to the master mix to prevent protein precipitation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardDenaturationReagent TargetConcentrations
For each member of Standards, indicates the final concentration of the denaturing agent in the mastermix.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
StandardDenaturationReagent Volumes
For each member of Standards, indicates the volumes of the denaturing agent required to reach its final concentration in the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardAmpholytes
For each member of Standards, indicates the makeup of amphoteric molecules in the master mix that form the pH gradient.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
StandardAmpholyteTarget Concentrations
For each member of Standards, indicates the concentrations (Vol/Vol) of amphoteric molecules in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
StandardAmpholyteVolumes
For each member of Standards, indicates the volumes of amphoteric molecule stocks to add to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
StandardIsoelectricPoint Markers
For each member of Standards, indicates the Reference analytes included in the mastermix. pI markers facilitate the interpolation of sample pI. The pH is then interpolated by the straight line between the pI markers.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
StandardIsoelectricPoint MarkersTargetConcentrations
For each member of Standards, indicates the final concentrations (Vol/Vol) of pI markers in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
StandardIsoelectricPoint MarkersVolumes
For each member of Standards, indicates the volume of pI marker stocks added to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
StandardAnodicSpacers
For each member of Standards, indicates the Acidic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardAnodicSpacerTarget Concentrations
For each member of Standards, indicates the final concentrations of AnodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
StandardAnodicSpacerVolumes
For each member of Standards, indicates the volume of AnodicSpacer stock added to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardCathodicSpacers
For each member of Standards, indicates the basic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardCathodicSpacerTarget Concentrations
For each member of Standards, indicates the final concentrations of CathodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
StandardCathodicSpacerVolumes
For each member of Standards, indicates the volume of CathodicSpacer stock to add to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardElectroosmoticFlow Blockers
For each member of Standards, indicates the solution added to samples to minimize electroOsmotic flow in the capillary.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardElectroosmoticFlow BlockerTargetConcentrations
For each member of Standards, indicates the final concentrations of ElectroosmoticFlowBlockers in the mastermix.
Format: Multiple
Class: Real
Unit: MassPercent
Programmatic Pattern: RangeP[0 MassPercent, 100 MassPercent]
StandardElectroosmoticFlow BlockerVolumes
For each member of Standards, indicates the volumes of ElectroosmoticFlowBlockers stock to add to the master mix for each sample.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardVoltageDuration Profiles
For each member of Standards, indicates the series of voltages and durations to apply onto the capillary for separation.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
StandardImagingMethods
For each member of Standards, indicates whole capillary imaging methods applied.
Format: Multiple
Class: Expression
Programmatic Pattern: Absorbance | AbsorbanceAndFluorescence
StandardNativeFluorescence ExposureTimes
For each member of Standards, indicates the exposure durations for NativeFluorescence detection.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterP[0 Second]..}
Blanks
Blanks
Known analytes that serve as Blanks for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankTotalVolumes
For each member of Blanks, TotalVolumes indicates the final volume in the assay tube prior to loading onto the capillary. Each tube contains a Sample, DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankVolumes
For each member of Blanks, indicates the volume of blank required.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankPremadeMasterMixReagents
For each member of Blanks, indicates the premade master mix used. The master mix contains the reagents required for cIEF experiments, i.e., DenaturationReagent, Ampholytes, IsoelectricPointMarkers, Spacers, and ElectroosmoticBlocker.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankPremadeMasterMixDiluents
For each member of Blanks, indicates the premade master mix diluent used. The diluent solution dilutes the premade master mix used to its working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankPremadeMasterMixReagent DilutionFactors
For each member of Blanks, indicates the factor by which the premade master mix needs to be diluted by.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterEqualP[1]
BlankPremadeMasterMixVolumes
For each member of Blanks, indicates the volume of the premade master mix required to reach its final concentration.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankDiluents
For each member of Blanks, indicates the diluent buffer, e.g., Milli-Q water, added to the master mix to dilute all components to working concentration.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankDenaturationReagents
For each member of Blanks, indicates the denaturing agent, e.g., Urea or SimpleSol, added to the master mix to prevent protein precipitation.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankDenaturationReagent TargetConcentrations
For each member of Blanks, indicates the final concentration of the denaturing agent in the mastermix.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterEqualP[0 Molar] | RangeP[0 VolumePercent, 100 VolumePercent]
BlankDenaturationReagent Volumes
For each member of Blanks, indicates the volumes of the denaturing agent required to reach its final concentration in the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankAmpholytes
For each member of Blanks, indicates the makeup of amphoteric molecules in the master mix that form the pH gradient.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
BlankAmpholyteTarget Concentrations
For each member of Blanks, indicates the concentrations (Vol/Vol) of amphoteric molecules in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
BlankAmpholyteVolumes
For each member of Blanks, indicates the volumes of amphoteric molecule stocks to add to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
BlankIsoelectricPointMarkers
For each member of Blanks, indicates the Reference analytes included in the mastermix. pI markers facilitate the interpolation of sample pI. The pH is then interpolated by the straight line between the pI markers.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
BlankIsoelectricPointMarkers TargetConcentrations
For each member of Blanks, indicates the final concentrations (Vol/Vol) of pI markers in the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {RangeP[0 VolumePercent, 100 VolumePercent]..}
BlankIsoelectricPointMarkers Volumes
For each member of Blanks, indicates the volume of pI marker stocks added to the mastermix.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Microliter]..}
BlankAnodicSpacers
For each member of Blanks, indicates the Acidic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankAnodicSpacerTarget Concentrations
For each member of Blanks, indicates the final concentrations of AnodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
BlankAnodicSpacerVolumes
For each member of Blanks, indicates the volume of AnodicSpacer stock added to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankCathodicSpacers
For each member of Blanks, indicates the basic ampholyte included in the mastermix. Both acidic and alkaline carrier ampholytes may be lost in the electrolyte reservoirs due to diffusion and isotachophoresis, decreasing the resolution and detection of proteins at the extremes of the pH gradient. To reduce the loss of ampholytes, Spacers (ampholytes with very low or very high pIs) can be added to buffer the loss of analytes of interest.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankCathodicSpacerTarget Concentrations
For each member of Blanks, indicates the final concentrations of CathodicSpacer in the mastermix.
Format: Multiple
Class: Real
Unit: Molar
Programmatic Pattern: GreaterEqualP[0 Molar]
BlankCathodicSpacerVolumes
For each member of Blanks, indicates the volume of CathodicSpacer stock to add to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankElectroosmoticFlow Blockers
For each member of Blanks, indicates the solution added to samples to minimize electroOsmotic flow in the capillary.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankElectroosmoticFlow BlockerTargetConcentrations
For each member of Blanks, indicates the final concentrations of ElectroosmoticFlowBlockers in the mastermix.
Format: Multiple
Class: Real
Unit: MassPercent
Programmatic Pattern: RangeP[0 MassPercent, 100 MassPercent]
BlankElectroosmoticFlow BlockerVolumes
For each member of Blanks, indicates the volumes of ElectroosmoticFlowBlockers stock to add to the mastermix.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
BlankLoadTimes
For each member of Blanks, indicates the time to load samples mixed with master mix into the capillary by vacuum.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterP[0 Second]
BlankVoltageDurationProfiles
For each member of Blanks, indicates the series of voltages and durations to apply onto the capillary for separation for each sample.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
BlankImagingMethods
For each member of Blanks, indicates the whole capillary imaging methods applied.
Format: Multiple
Class: Expression
Programmatic Pattern: Absorbance | AbsorbanceAndFluorescence
BlankNativeFluorescence ExposureTimes
For each member of Blanks, indicates the exposure duration for NativeFluorescence detection.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterP[0 Second]..}
Last modified on Mon 26 Sep 2022 15:43:49