ecl-logo Documentation

Object[Protocol, DynamicFoamAnalysis]

A protocol for running a Dynamic Foam Analysis experiment on a set of samples.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Protocol, DynamicFoamAnalysis, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].

    Author

    The investigator who created this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

General

    DateConfirmed

    The date on which the protocol first entered processing or a backlog.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateEnqueued

    The date on which the protocol was accepted and added to the queue.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateStarted

    The date on which the protocol moved from the front of the queue to begin running in the lab.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateCompleted

    The date on which the last step of this protocol's execution was finished.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    DateCanceled

    The date on which the protocol was canceled and removed from the queue.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    Script

    The script that created this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    SubprotocolScripts

    All scripts generated by this protocol as a list of subprotocols.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    Site

    The ECL site at which this protocol was executed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    SamplesIn

    Input samples for this analytical or preparative experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ContainersIn

    Any containers containing this protocols' SamplesIn.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    NumberOfReplicates

    Indicates the number of times the experiment is repeated with identical parameters.
    Format: Single
    Class: Integer
    Programmatic Pattern: GreaterP[0, 1]

    Status

    The current status of the protocol.
    Format: Single
    Class: Expression
    Programmatic Pattern: ProtocolStatusP

    CheckpointProgress

    A listing of all the checkpoints passed in the execution of this protocol.
    Format: Multiple

    [[1]] Name

      Header: Name
      Class: String
      Programmatic Pattern: _String

    [[2]] Start Time

      Header: Start Time
      Class: Date
      Programmatic Pattern: _?DateObjectQ

    [[3]] End Time

      Header: End Time
      Class: Date
      Programmatic Pattern: _?DateObjectQ|Null

    SubprotocolDescription

    A short title indicating what this subprotocol accomplishes.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    BatchedUnitOperations

    The individual batches of the protocol, as they will be executed in the lab.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ReplacementProtocol

    If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    Sterile

    Indicates if the protocol is performed in a sterile environment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    LiquidHandlingLog

    The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    PooledSamplesIn

    The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}

    NestedIndexMatchingSamplesIn

    The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}

    InitialNitrogenPressure

    The pressure data of the nitrogen gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    NitrogenPressureLog

    The pressure log for the nitrogen gas source for the relevant section of the facility.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialCO2Pressure

    The pressure data of the CO2 gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CO2PressureLog

    The pressure log for the CO2 gas source for the relevant section of the facility.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InitialArgonPressure

    The pressure data of the argon gas source before starting the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    HoldOrder

    Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    Priority

    Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    StartDate

    The date at which this protocol should be prioritized to start.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    Branch

    The git branch that this protocol should be run on.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    SampleVolume

    For each member of SamplesIn, the SampleVolume of the samples that will be used in the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 milliliter
    Programmatic Pattern: GreaterEqualP[0 Milliliter]

    Temperature

    For each member of SamplesIn, the temperature at which the foam column containing the sample will be heated to during the duration of the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterEqualP[0 Celsius]

    TemperatureMonitoring

    For each member of SamplesIn, indicates if the temperature of the sample will be directly monitored during the experiment using a probe inserted into the foam column.
    Format: Multiple
    Class: Boolean
    Programmatic Pattern: BooleanP

    DetectionMethod

    For each member of SamplesIn, the type of foam detection method(s) that will be used during the experiment. The foam detection methods are the Height Method (default method for the Dynamic Foam Analyzer), Liquid Conductivity Method, and Imaging Method. The Height Method provides information on foamability and foam height, the Liquid Conductivity Method provides information on the liquid content and drainage dynamics of foam, and then Imaging Method provides data on the size and distribution of foam bubbles.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: {FoamDetectionMethodP..}

    FoamColumnLoading

    For each member of SamplesIn, indicates whether the foam column will be pre-wetted when the sample is loaded during the experiment. Wet indicates that the sides of the foam column will be wetted with the sample during sample loading. Dry indicates that the sample will be directly loaded to the bottom of the foam column, and the sides of the column will be left dry.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Wet | Dry

    StructureIllumination Intensity

    For each member of SamplesIn, the illumination intensity that will be used for foam structure detection by the Foam Structure Module.
    Format: Multiple
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: GreaterEqualP[0 Percent]

    FieldOfView

    For each member of SamplesIn, the size of the surface area that is observable at any given moment by the camera used by the Foam Imaging Module in the experiment if the Imaging Method is selected.
    Format: Multiple
    Class: Integer
    Unit: 1 millimeter squared
    Programmatic Pattern: 2 2 2 Hold[85 Millimeter | 140 Millimeter | 285 Millimeter ]

    Agitation

    For each member of SamplesIn, the type of agitation (sparging or stirring) used for foam generation of the sample.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: FoamAgitationTypeP

    AgitationTime

    For each member of SamplesIn, the amount of time the dynamic foam analysis experiment will agitate the sample to induce the production of foam.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterEqualP[0 Second]

    AgitationSamplingFrequency

    For each member of SamplesIn, the data sampling frequency during the agitation period in which foam is made. The data recorded for the Height Method are the foam and liquid heights over time. The data recorded for the Imaging Method are timelapse 2D snapshots of the foam in the camera field of view. The data recorded for the Liquid Conductivity Method are the resistances at sensors spaced along the length of the foam column.
    Format: Multiple
    Class: Real
    Unit: 1 unity per second
    Programmatic Pattern: Unit Hold[GreaterP[0 ------]] Second

    SpargeGas

    For each member of SamplesIn, the sparging gas that will be used during foam generation in the experiment if agitation is set to sparge.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: FoamSpargeGasP

    SpargeFlowRate

    For each member of SamplesIn, the flow rate of the sparging gas that will be used during foam generation in the experiment if agitation is set to sparge.
    Format: Multiple
    Class: Real
    Unit: 1 liter per minute
    Programmatic Pattern: Liter Hold[GreaterEqualP[0 ------]] Minute

    StirRate

    For each member of SamplesIn, the stir rate of the stir blade that will be used during foam generation in the experiment if agitation is set to stir.
    Format: Multiple
    Class: Integer
    Unit: 1 revolution per minute
    Programmatic Pattern: GreaterEqualP[0 RPM]

    StirOscillationPeriod

    For each member of SamplesIn, the oscillation period setting for the stir blade that will be used during foam generation in the experiment if agitation is set to stir. This refers to the time after which the stirring blade changes stirring direction.
    Format: Multiple
    Class: Integer
    Unit: 1 second
    Programmatic Pattern: GreaterEqualP[0 Second]

    DecayTime

    For each member of SamplesIn, the amount of time the dynamic foam analysis experiment will allow the foam bubbles to drain and coalesce, during which experimental measurements will be taken.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterEqualP[0 Minute]

    DecaySamplingFrequency

    For each member of SamplesIn, the data sampling frequency during the period in which the foam column undergoes decay, involving liquid draining, bubble coalescence, and foam column height decrease. The data recorded for the Height Method are the foam and liquid heights over time. The data recorded for the Imaging Method are timelapse 2D snapshots of the foam in the camera field of view. The data recorded for the Liquid Conductivity Method are the resistances at sensors spaced along the length of the foam column.
    Format: Multiple
    Class: Real
    Unit: 1 unity per second
    Programmatic Pattern: Unit Hold[GreaterP[0 ------]] Second

    Wavelength

    For each member of SamplesIn, the wavelength type (visible or infrared) that will be used by the Diode Array Module during the experiment if the Diode Array Module detector is selected.
    Format: Multiple
    Class: Integer
    Unit: 1 nanometer
    Programmatic Pattern: 469 Nanometer | 850 Nanometer

    HeightIlluminationIntensity

    For each member of SamplesIn, the illumination intensity that will be used for foam height detection by the Diode Array Module.
    Format: Multiple
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: GreaterEqualP[0 Percent]

Replicate Experiments

    Template

    The protocol whose option selections should be selected as the default options for this protocol.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    ProtocolsTemplated

    Protocols which were generated using this protocol's options as a starting point for its option defaults.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Sample Preparation

    PreparatoryUnitOperations

    A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: _ | _

    PreparatoryPrimitives

    A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: _ | _

    VolumeCheckSamplePrep

    Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    IncubateSamplePreparation

    For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    CentrifugeSamplePreparation

    For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    FilterSamplePreparation

    For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
    Format: Multiple

    [Filtration]

      Header: Filtration
      Class: Boolean

    [FiltrationType]

      Header: FiltrationType
      Class: Expression

    [Filter]

    [FilterMaterial]

      Header: FilterMaterial
      Class: Expression

    [FilterPoreSize]

      Header: FilterPoreSize
      Class: Real
      Unit: 1 micrometer

    [FilterContainerOut]

      Header: FilterContainerOut
      Class: Expression

    [FilterInstrument]

    [FilterSyringe]

    [FilterHousing]

    [FilterIntensity]

      Header: FilterIntensity
      Class: VariableUnit

    [FilterTime]

      Header: FilterTime
      Class: Real
      Unit: 1 minute

    [FilterTemperature]

      Header: FilterTemperature
      Class: Real
      Unit: 1 degree Celsius

    [FilterSterile]

      Header: FilterSterile
      Class: Boolean

    [FilterAliquotContainer]

      Header: FilterAliquotContainer
      Class: Expression

    [FilterAliquot]

      Header: FilterAliquot
      Class: Real
      Unit: 1 liter

    [PrefilterMaterial]

      Header: PrefilterMaterial
      Class: Expression

    [PrefilterPoreSize]

      Header: PrefilterPoreSize
      Class: Real
      Unit: 1 micrometer

    [FilterAliquotDestinationWell]

      Header: FilterAliquotDestinationWell
      Class: Expression

    SamplePreparationProtocols

    Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    PooledIncubateSample Preparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    NestedIndexMatchingIncubate SamplePreparation

    For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
    Format: Multiple

    [Incubate]

      Header: Incubate
      Class: Boolean

    [IncubationTemperature]

      Header: IncubationTemperature
      Class: Real
      Unit: 1 degree Celsius

    [IncubationTime]

      Header: IncubationTime
      Class: Real
      Unit: 1 second

    [Mix]

      Header: Mix
      Class: Boolean

    [MixType]

      Header: MixType
      Class: Expression

    [MixUntilDissolved]

      Header: MixUntilDissolved
      Class: Boolean

    [MaxIncubationTime]

      Header: MaxIncubationTime
      Class: Real
      Unit: 1 second

    [IncubationInstrument]

    [AnnealingTime]

      Header: AnnealingTime
      Class: Real
      Unit: 1 second

    [IncubateAliquotContainer]

      Header: IncubateAliquotContainer
      Class: Expression

    [IncubateAliquot]

      Header: IncubateAliquot
      Class: Real
      Unit: 1 liter

    [IncubateAliquotDestinationWell]

      Header: IncubateAliquotDestinationWell
      Class: Expression

    PooledCentrifugeSample Preparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    NestedIndexMatchingCentrifuge SamplePreparation

    For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
    Format: Multiple

    [Centrifuge]

      Header: Centrifuge
      Class: Boolean

    [CentrifugeInstrument]

    [CentrifugeIntensity]

      Header: CentrifugeIntensity
      Class: VariableUnit

    [CentrifugeTime]

      Header: CentrifugeTime
      Class: Real
      Unit: 1 minute

    [CentrifugeTemperature]

      Header: CentrifugeTemperature
      Class: Real
      Unit: 1 degree Celsius

    [CentrifugeAliquotContainer]

      Header: CentrifugeAliquotContainer
      Class: Expression

    [CentrifugeAliquot]

      Header: CentrifugeAliquot
      Class: Real
      Unit: 1 liter

    [CentrifugeAliquotDestinationWell]

      Header: CentrifugeAliquotDestinationWell
      Class: Expression

    FilteredSamples

    Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample]

Aliquoting

    AliquotSamplePreparation

    For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
    Format: Multiple

    [Aliquot]

      Header: Aliquot
      Class: Boolean

    [AliquotAmount]

      Header: AliquotAmount
      Class: Expression

    [TargetConcentration]

      Header: TargetConcentration
      Class: Expression

    [AssayVolume]

      Header: AssayVolume
      Class: Real
      Unit: 1 milliliter

    [AliquotContainer]

      Header: AliquotContainer
      Class: Expression

    [AssayBuffer]

    [BufferDiluent]

    [BufferDilutionFactor]

      Header: BufferDilutionFactor
      Class: Real

    [ConcentratedBuffer]

    [DestinationWell]

      Header: DestinationWell
      Class: Expression

    [TargetConcentrationAnalyte]

      Header: TargetConcentrationAnalyte
      Class: Expression

    [AliquotSampleLabel]

      Header: AliquotSampleLabel
      Class: String

    AliquotProtocols

    Protocols used to create AliquotSamples prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    AliquotPreparationProtocols

    Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    AliquotSamples

    Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample]

    AliquotContainers

    Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SaveAliquots

    Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    ConsolidateAliquots

    Indicates if identical aliquots should be consolidated in a single sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    AliquotPreparation

    Indicates if the aliquotting should occur manually or on a robotic liquid handler.
    Format: Single
    Class: Expression
    Programmatic Pattern: PreparationMethodP

    AliquotVolumes

    For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterP[0 Micro Liter]

    AliquotMasses

    For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 milligram
    Programmatic Pattern: GreaterP[0 Milligram]

    AliquotAmounts

    For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: VariableUnit
    Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]

    TargetConcentrations

    For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Milligram Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]] Milliliter

    AssayVolumes

    For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
    Format: Multiple
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Micro Liter]

    AssayBuffers

    For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ConcentratedBuffers

    For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    BufferDilutionFactors

    For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Real
    Programmatic Pattern: GreaterP[0]

    BufferDiluents

    For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ConcentratedBuffer

    The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    BufferDilutionFactor

    The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Integer
    Programmatic Pattern: GreaterP[0]

    BufferDiluent

    The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    AssayBuffer

    The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

Sample Post-Processing

    PostProcessingProtocols

    Protocols used to perform post-processing such as sample imaging or volume measurement.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ImageSample

    Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    MeasureVolume

    Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    MeasureWeight

    Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    Measure

    Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    Storage

    Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
    Format: Single
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Experimental Results

Troubleshooting

    TroubleshootingReports

    Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Resources

    AwaitingResources

    Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    ShippingMaterials

    Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
    Format: Multiple

    [[1]] Transaction

    [[2]] Required Product

    HazardousWaste

    The types of waste generated by this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    DispenserContainers

    Containers gathered as destinations for any resources obtained from a chemical dispenser.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    GasSources

    A list of containers that supplied gas to instruments in this protocol through plumbing connections.
    Format: Multiple

    [[1]] Gas Type

      Header: Gas Type
      Class: Expression
      Programmatic Pattern: GasP

    [[2]] Source Container

      Header: Source Container
      Class: Link
      Programmatic Pattern: _Link

    [[3]] Supplied Instrument

      Header: Supplied Instrument
      Class: Link
      Programmatic Pattern: _Link

Cleaning

    WasteGenerated

    For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
    Format: Multiple

    [Waste]

    [Weight]

      Header: Weight
      Class: Real
      Unit: 1 gram

Storage Information

    StoragePrice

    The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
    Format: Single
    Class: Real
    Unit: 1 US dollar per month
    Programmatic Pattern: USD Hold[GreaterEqualP[0 -----]] Month

Sample Storage

    SamplesInStorage

    For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

    AliquotStorage

    For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

    SamplesOutStorage

    For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: SampleStorageTypeP | Disposal

Option Handling

    ResolvedOptions

    The final options used as parameters for this protocol, after automatic options are resolved.
    Format: Single
    Class: Compressed
    Programmatic Pattern: {_Rule...}

    UnresolvedOptions

    The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
    Format: Single
    Class: Compressed
    Programmatic Pattern: {_Rule...}

Computations

    ParallelComputations

    List of computational jobs generated and run in parallel to the execution of this protocol.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ComputationsOutstanding

    Indicates if any of the computation jobs in ParallelComputations have not yet completed.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    ErroneousComputations

    List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Instrument Specifications

    Instrument

    For each member of SamplesIn, the dynamic foam analyzer instrument that should be used in this experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FoamColumn

    For each member of SamplesIn, the foam column used to contain the sample during the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    LiquidConductivityModule

    For each member of SamplesIn, the Liquid Conductivity Module object that will be used in the experiment if the Liquid Conductivity Method is selected. The Liquid Conductivity Module is an attachment for the Dynamic Foam Analyzer instrument that provides information on the liquid content and drainage dynamics over time at various positions along the foam column; this is achieved by recording changes in conductivity of the foam over time, which provides information on the amount of liquid vs gas that is present.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    FoamImagingModule

    For each member of SamplesIn, the Foam Imaging Module object that will be used in the experiment if the Imaging Method is selected. The Foam Imaging Module involves transmitting light through a glass prism specially fitted on the side of a foam column, in order to ascertain the 2D structure of the foam based on the principles of total reflection. Since glass and liquid have comparable diffractive indices, light that hits a foam lamella will be partially diffracted and transmitted into the foam. On the other hand, glass and air have different diffractive indices, so light that hits within the air bubble will be fully reflected and sensed by the camera, allowing for construction of a 2D image of the layer of foam located at the edge of the prism.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    DiodeArrayModuleIR

    The infrared light Diode Array Module object that will be used in the experiment if a Wavelength of 850 nanometers is selected. The infrared Diode Array Module uses infrared light in order to ascertain the boundaries between liquid, foam, and air, for opaque samples.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    CameraHeight

    For each member of SamplesIn, the height along the column at which the camera used by the Foam Imaging Module will be positioned during the experiment if the Imaging Method is selected.
    Format: Multiple
    Class: Integer
    Unit: 1 millimeter
    Programmatic Pattern: GreaterEqualP[0 Millimeter]

    SpargeFilter

    For each member of SamplesIn, the sparging filter that will be used to introduce gas bubbles into the column during foam generation. The filter is a glass plate with pores for bubble generation. The size of the filter must match the size of the column selected in order to only flow gas within the confines of the column.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    StirBlade

    For each member of SamplesIn, the stir blade that will be used during foam generation in the experiment if agitation is set to stir.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    NumberOfWashes

    For each member of SamplesIn, the number of washes that will be used to clean the column and/or the stir blade/filter plate after the experiment is run.
    Format: Multiple
    Class: Integer
    Programmatic Pattern: GreaterEqualP[1, 1]

    Wipes

    For each member of SamplesIn, indicates the cleaning wipes that will be used to dry the column and/or the impeller blade/filter plate after the experiment is run.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    WasteContainer

    The vessel used to contain the rinse water from the cleaning steps.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    Syringes

    For each member of SamplesIn, the syringes used to load the sample into the foam columns.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    Needles

    For each member of SamplesIn, the needle used to extend the syringe for loading the sample into the foam column.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SpargeFilterCleaner

    For each member of SamplesIn, the SpargeFilterCleaner that will be used to clean the filter plate after the experiment, if the Sparge Agitation method was used.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    RecirculatingPump

    The pump that chills or heats fluid and flows it through the temperature control column in order to regulate the temperature during the experiment.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    FoamAgitationModule

    For each member of SamplesIn, the agitation module that will be used to hold the foam column, agitation unit, and sample during the experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    ORing

    For each member of SamplesIn, the O-ring which seals the connection between the foam column and the foam agitation module, to be installed with either the sparge filter or stir blade during this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SecondaryORing

    For each member of SamplesIn, the secondary O-ring which seals the connection between the foam column and the foam agitation module, to be installed with either the sparge filter or stir blade during this experiment.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
Last modified on Mon 26 Sep 2022 15:43:49