Object[Protocol, ELISA]
A protocol for performing a Enzyme-Linked Immunosorbent Assay (ELISA) experiment on the provided samples for the detection and quantification of an analyte using antibodies.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, ELISA, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, ELISA]
Pattern Description: Object[Protocol, ELISA]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLogs
The instrumentation trace files that monitored and recorded the execution of this robotic liquid handling.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The detection and quantification ELISA device on which the protocol is used to perform the ELISA experiment and detects the signals to quantify certain analytes.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[{Object[Instrument, LiquidHandler], Model[Instrument, LiquidHandler]}]
ELISALiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling by the ELISA Instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Method
Defines the type of ELISA experiment to be performed. Types include DirectELISA, IndirectELISA, DirectSandwichELISA, IndirectSandwichELISA, DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA. In a DirectELISA experiment, the Sample is coated on the ELISAPlate, and then a primary antibody is used to detect the target antigen. In a DirectSandwichELISA experiment, a capture antibody is coated onto the ELISAPlate to pull down the target antigen from the sample. Then primary antibody is used to detect the target antigen. In a DirectCompetitiveELISA and IndirectCompetitiveELISA experiment, a reference antigen is used to coat the ELISAPlate. Samples are incubated with the primary antibody. Then, when the Sample-Antibody-Complex solution is loaded on the ELISAPlate, the remaining free primary antibody binds to the reference antigen. In a FastELISA experiment, a coating antibody against a small protein tag is coated to the ELISAPlate. A capture antibody containing this small tag and a primary antibody for antigen detection is incubated with the sample to form a CaptureAntibody-TargetAntigen-PrimaryAntibody complex. Then this complex is pulled down by the coating antibody to the surface of the plate. Compared with the Direct methods where the primary antibody is conjugated with an enzyme (such as Horse Radish Peroxidase/HRP or Alkaline Phosphatas/AP) for detection, in Indirect methods a secondary antibody is, instead, conjugated with this enzyme. During Indirect methods, an additional step of SecondaryAntibody incubation is added to the corresponding Direct methods.
Format: Single
Class: Expression
Programmatic Pattern: ELISAMethodP
TargetAntigens
For each member of SamplesIn, the Analyte molecule(e.g., peptide, protein, and hormone) detected and quantified in the samples by Antibodies in the ELISA experiment. This option is used to automatically set sample Antibodies and the corresponding experiment conditions of Standards and Blanks.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Model[Molecule]]
Relation: Model[Molecule]
WashingBuffer
The solution used to rinse off unbound molecules from the assay plate.
Format: Single
Class: Link
SampleAssemblyPrimitives
A set of SampleManipulation instructions specifying the dilution, spiking, and antibody mixings of Samples, Standards, and Blanks.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
AntibodyAntigenDilution Primitives
A set of SampleManipulation instructions specifying the dilution of antibodies and reference antigen using their corresponding diluents.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
CoatingPlateAssembly Primitives
A set of SampleManipulation instructions specifying the coating of ELISAPlate and SecondaryELISAPlate.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
ELISAPrimitives
A set of SampleManipulation instructions specifying the blocking, immunosorbent steps, substratrate incubation, and plate reading performed in the ELISA instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: ELISAPrimitivesP
SampleAssemblyProtocol
The sample manipulation protocol used for the dilution, spiking, and antibody mixings of Samples, Standards, and Blanks.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
AntibodyAntigenDilution Protocol
The sample manipulation protocol used for the dilution of antibodies and reference antigen using their corresponding diluents.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
CoatingPlateAssemblyProtocol
The sample manipulation protocol used for transfering the solutions for the coating of ELISAPlate and SecondaryELISAPlate.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol, SampleManipulation]]
Relation: Object[Protocol, SampleManipulation]
CoatingPlateIncubation Protocol
The ExperimentIncubation protocol used for the coating of ELISAPlate and SecondaryELISAPlate.
Format: Single
Class: Link
Programmatic Pattern: ObjectP[Object[Protocol]]
Relation: Object[Protocol]
SampleAntibodyComplex Incubation
Indicates if the pre-mixed sample and Antibodies should be incubated before loaded into the assay plate.
Format: Single
Class: Expression
Programmatic Pattern: Alternatives[BooleanP]
Coating
Indicates if Coating is required. Coating is a procedure to non-sepcifically adsorb protein molecules to the surface of wells of an assay plate.
Format: Single
Class: Expression
Programmatic Pattern: Alternatives[BooleanP]
PrereadTimepoints
The list of time points when the absorbance intensities were recorded at PrereadAbsorbanceWavelengths during the preread process.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterP[0 Minute]
LiquidHandlingPressureLog
The instrumentation trace file that monitored and recorded the pressure curves during aspiration and dispense of this ELISA liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
Spikes
For each member of SamplesIn, the Spike sample with a known concentration of analyte to be mixed with the input sample. The purpose of spiking is to perform a spike-and-recovery assessment to determine whether the ELISA can accurately test the concentration of analyte within the context of the sample.Spike-and-Recovery experiment is used to test whether the ELISA can reliably measure TargetAntigen concentration in the context of the sample. For example, if molecules in the sample inhibits the binding between the antibody and TargetAntigen, the results from the ELISA becomes inaccurate. In a Spike-and-Recovery experiment,an aliquote of a sample (in a different wells than the main ELISA experiment) is mixed with Spike (a sample containing known amount of TargetAntigen, the Standard sample can be used). ELISA is performed on the Sample alone (also called Neat Sample) and the Spiked Sample at the same time, where the Spike concentration in the sample can be measured. This measured Spike concentration can be then compared with the known Spike concentration. Typically a 20% difference between measured Spike concentration and the known Spike concentration is acceptable. Spiked sample can be further diluted to perform linearity-of-dilution assessment.
Format: Multiple
Class: Link
SpikeDilutionFactors
For each member of SamplesIn, the ratio of dilution by which the Spike is mixed with the input sample before further dilution is performed.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
SampleDilutionCurves
For each member of SamplesIn, the multi-step dilutions of (spiked) sample, presented as {Sample transfer volume, Diluent transfer volume}.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
SampleSerialDilutionCurves
For each member of SamplesIn, the multi-step serial dilutions of (spiked) sample, presented as {Transfer volume of previous dilution, Diluent transfer volume}.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
SampleDiluent
The buffer used to perform multiple dilutions of samples or spiked samples.
Format: Single
Class: Link
SampleAssemblyPlate
The deep well plate used to dilute samples, standards, spiking samples, mixing samples and antibodies, and antibody-sample complex incubation before loading them onto ELISAplate.
Format: Single
Class: Link
SecondarySampleAssemblyPlate
The deep well plated used to dilute samples, standards, spiking samples, mixing samples and antibodies, and antibody-sample complex incubation before loading them onto the SecondaryELISAPlate.
Format: Single
Class: Link
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
MethodFile
The json file containing the run information generated for the ELISA experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
DataFiles
The txt files containing the experiment data generated by the ELISA instrument.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PrereadDataFiles
For each member of PrereadTimepoints, the txt files containing the experiment data generated by the ELISA instrument.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
StandardData
Data of Standards generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
BlankData
Data of Blanks generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
PrereadData
Preread data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ReadyCheckComputations
The list of manifold job notebooks that was used to evaluate ReadyCheck for this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Job]
Antibody Antigen Preparation
SpikeStorageConditions
For each member of SamplesIn, the condition under which the unused portion of Spike stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CoatingAntibodies
For each member of SamplesIn, the sample containing the antibody that is used in for coating in FastELISA.
Format: Multiple
Class: Link
CoatingAntibodyDilution Factors
For each member of SamplesIn, the ratio of dilution of CoatingAntibody. CoatingAntibody is diluted with CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
CoatingAntibodyVolumes
For each member of SamplesIn, the volume of Coating added into the corresponding well of the assay plate. CoatingAntibody is diluted with CoatingBuffer. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
CoatingAntibodyDiluent
The buffer used to dilute CoatingAntibodies.
Format: Single
Class: Link
CoatingAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of Coating Antibody stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CaptureAntibodies
For each member of SamplesIn, the sample containing the antibody that is used to pull down the antigen from sample solution to the surface of the assay plate wll in DirectSandwichELISA, IndirectSandwichELISA, and FastELISA.
Format: Multiple
Class: Link
CaptureAntibodyDilution Factors
For each member of SamplesIn, the ratio of dilution of CaptureAntibody. For DirectSandwichELISA and IndirectSandwichELISA, CaptureAntibody is diluted with CoatingBuffer. For FastELISA, CaptureAntibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
CaptureAntibodyVolumes
For each member of SamplesIn, the volume of CaptureAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
CaptureAntibodyDiluent
The buffer used to dilute CaptureAntibodies. If Null, CaptureAntibody will be diluted into the corresponding Sample.
Format: Single
Class: Link
CaptureAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of Capture Antibody stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ReferenceAntigens
For each member of SamplesIn, the SAMPLE containing the antigen that is used in DirectCompetitiveELISA or IndirectCompetitiveELISA. The ReferenceAntigen competes with sample antigen for the binding of the PrimaryAntibody. Reference Antigen is sometimes also referred to as Inhibitor Antigen.
Format: Multiple
Class: Link
ReferenceAntigenDilution Factors
For each member of SamplesIn, the ratio of dilution of ReferenceAntigen. The ReferenceAntigenSample is always diluted in CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
ReferenceAntigenVolumes
For each member of SamplesIn, the volume of ReferenceAntigen added into the corresponding well of the assay plate. ReferenceAntigenVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
ReferenceAntigenDiluent
The buffer used to dilute the ReferenceAntigen. If Null, ReferenceAntigen will be mixed with the corresponding sample.
Format: Single
Class: Link
ReferenceAntigenStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of Reference Antigen stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
PrimaryAntibodies
For each member of SamplesIn, the antibody that directly binds with the TargetAntigen (analyte).
Format: Multiple
Class: Link
PrimaryAntibodyDilution Factors
For each member of SamplesIn, the ratio of dilution of PrimaryAntibody. For DirectELISA, IndirectELISA, DirectSandwichELISA, and IndirectSandwichELISA, the antibody is diluted with ImmunosorbentBuffer. For DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA, the antibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
PrimaryAntibodyVolumes
For each member of SamplesIn, the volume of PrimaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
PrimaryAntibodyDiluent
The buffer used to dilute PrimaryAntibodies.
Format: Single
Class: Link
PrimaryAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of Primary Antibody stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SecondaryAntibodies
For each member of SamplesIn, the antibody that binds to the primary antibody.
Format: Multiple
Class: Link
SecondaryAntibodyDilution Factors
For each member of SamplesIn, the ratio of dilution of SecondaryAntibody. SecondaryAntibody is always diluted in the ImmunosorbentBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
SecondaryAntibodyVolumes
For each member of SamplesIn, the volume of SecondaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
SecondaryAntibodyDiluent
The buffer used to dilute CoatingAntibodies.
Format: Single
Class: Link
SecondaryAntibodyStorage Conditions
For each member of SamplesIn, the condition under which the unused portion of Secondary Antibody stock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
PrimaryAntibodyConcentrates
All of the primary antibody resources that need to be diluted.
Format: Multiple
Class: Link
PrimaryAntibodyDilution Volumes
The volumes of each of the primary antibody that need to be diluted.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PrimaryAntibodyDiluent DilutionVolumes
The total volumes of the primary antibody diluent needed to dilute each primary antibody.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PrimaryAntibodyDilution Containers
The containers used to dilute primary antibodies.
Format: Multiple
Class: Link
PrimaryAntibodyConcentrate StorageConditions
The condition under which the unused portion of PrimaryAntibodyConcentrate samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SecondaryAntibodyConcentrates
All of the secondary antibody resources that need to be diluted.
Format: Multiple
Class: Link
SecondaryAntibodyDilution Volumes
The total volumes of the secondary antibodies that need to be diluted.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SecondaryAntibodyDiluent DilutionVolumes
The total volumes of the secondary antibody diluents needed to dilute each secondary antibody.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SecondaryAntibodyDilution Containers
The containers used to dilute secondary antibodies.
Format: Multiple
Class: Link
SecondaryAntibodyConcentrate StorageConditions
The condition under which the unused portion of SecondaryAntibodyConcentrate samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CaptureAntibodyConcentrates
All of the capture antibody resources that need to be diluted.
Format: Multiple
Class: Link
CaptureAntibodyDilution Volumes
The volumes of the capture antibodies that need to be diluted.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CaptureAntibodyDiluent DilutionVolumes
The volume of capture antibody diluent added into the dilution container.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CaptureAntibodyDilution Containers
The containers used to dilute capture antibodies.
Format: Multiple
Class: Link
CaptureAntibodyConcentrate StorageConditions
The condition under which the unused portion of CaptureAntibodyConcentrate samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
CoatingAntibodyConcentrates
All of the coating antibody resources that need to be diluted.
Format: Multiple
Class: Link
CoatingAntibodyDilution Volumes
The volumes of the coating antibodies that need to be diluted.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CoatingAntibodyDiluent DilutionVolumes
The volume of the coating antibody diluent added into the dilution container.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
CoatingAntibodyDilution Containers
The containers used to dilute coating antibodies.
Format: Multiple
Class: Link
CoatingAntibodyConcentrate StorageConditions
The condition under which the unused portion of CoatingAntibodyConcentrate samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ReferenceAntigenConcentrates
All of the reference antigen resources that need to be diluted.
Format: Multiple
Class: Link
ReferenceAntigenDilution Volumes
The volumes of the reference antigens that need to be diluted.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ReferenceAntigenDiluent DilutionVolumes
The volume of reference antigen diluent added into the dilution container.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ReferenceAntigenDilution Containers
The containers used to dilute reference antigens.
Format: Multiple
Class: Link
ReferenceAntigenConcentrate StorageConditions
The condition under which the unused portion of ReferenceAntigenConcentrate samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Sample Antibody Complex Incubation
SampleAntibodyComplex IncubationTime
The duration of SampleAntibodyComplex Incubation (If needed). In DirectCompetitiveELISA and IndirectCompetitiveELISA, PrimaryAntibody is incubated with the sample. In FastELISA, PrimaryAntibody and CaptureAntibody is incubated with the sample. If Null, the prepared sample-antibody complex will be kept at 4 degree Celsius till ready to use.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
SampleAntibodyComplex IncubationTemperature
The temperature at which sample mixed with Antibodies are incubated. In DirectCompetitiveELISA and IndirectCompetitiveELISA, PrimaryAntibody is incubated with the sample. In FastELISA, PrimaryAntibody and CaptureAntibody is incubated with the sample. If Null, the prepared sample-antibody complex will be kept at 4 degree Celsius till ready to use.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
Coating
SampleCoatingVolumes
For each member of SamplesIn, the amount of Sample that is aliquoted into the assay plate, in order for the Sample to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
CoatingAntibodyCoatingVolumes
For each member of SamplesIn, the amount of diluted CoatingAntibody that is aliquoted into the assay plate, in order for the CoatingAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 300 Microliter]
ReferenceAntigenCoating Volumes
For each member of SamplesIn, the amount of ReferenceAntigen that is aliquoted into the assay plate, in order for the ReferenceAntigen to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
CaptureAntibodyCoatingVolumes
For each member of SamplesIn, the amount of diluted CaptureAntibody that is aliquoted into the assay plate, in order for the CaptureAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
CoatingTemperature
The temperature at which the Coating Solution is kept in the assay plate, in order for the coating molecules to be adsorbed to the surface of the well.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
CoatingTime
The duration when the Coating Solution is kept in the assay plate, in order for the coating molecules to be adsorbed to the surface of the well.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Hour, 20 Hour]
CoatingWashVolume
The volume of WashBuffer added for to rinse off unbound molecule.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
CoatingNumberOfWashes
The number of washes performed after coating.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
StandardCaptureAntibody CoatingVolumes
For each member of Standards, the amount of diluted CaptureAntibody that is aliquoted into the ELISAPlate, in order for the CaptureAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
Blocking
Blocking
Indicates if a protein solution should be incubated with the assay plate to prevent non-specific binding of molecules to the assay plate.
Format: Single
Class: Expression
Programmatic Pattern: Alternatives[BooleanP]
BlockingBuffer
The protein-containing solution used to prevent non-specific binding of antigen or antibody to the surface of the assay plate.
Format: Single
Class: Link
BlockingVolumes
For each member of SamplesIn, the amount of BlockingBuffer that is aliquoted into the appropriate wells of the assay plate, in order to prevent non-specific binding of molecules to the assay plate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
BlockingTime
The duration when the Blocking Solution is kept with the assay plate, in order to prevent non-specific binding of molecules to the assay plate.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Hour, 20 Hour]
BlockingTemperature
The duration of time when the Blocking Solution is kept with the assay plate, in order to prevent non-specific binding of molecules to the assay plate.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
BlockingMixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Blocking incubation. Mixing is not recommended particularly when incubation volume is high, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
BlockingWashVolume
The volume of WashBuffer added after Blocking, in order to rinse off the unbound molecules from the surface of the wells.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
BlockingNumberOfWashes
The number of washes performed after Blocking, in order to rinse off the unbound molecules from the surface of the wells.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
Immunosorbent Step
SampleAntibodyComplex ImmunosorbentVolumes
For each member of SamplesIn, the volume of the SampleAntibodyComplex to be loaded on the ELISAPlate. In DirectCompetitiveELISA and IndirectCompetitiveELISA, this step enables the free primary antibody to bind to the ReferenceAntigen coated on the plate. In FastELISA, this step enables the PrimaryAntibody-TargetAntigen-CaptureAntibody complex to bind to the CoatingAntibody on the plate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
SampleAntibodyComplex ImmunosorbentTime
The duration of SampleAntibodyComplex incubation.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
SampleAntibodyComplex ImmunosorbentTemperature
The temperature of the SampleAntibodyComplex incubation.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
SampleAntibodyComplex ImmunosorbentMixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during SampleAntibodyComplex incubation in the ELISA Plate. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
SampleAntibodyComplex ImmunosorbentWashVolume
The volume of WashBuffer added to rinse off the unbound primary antibody after SampleAntibodyComplex incubation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
SampleAntibodyComplex ImmunosorbentNumberOfWashes
The number of rinses performed after SampleAntibodyComplex incubation.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
SampleImmunosorbentVolumes
For each member of SamplesIn, the volume of the Sample to be loaded on the ELISAPlate for the target antigen to bind to the capture antibody in DirectSandwichELISA and IndirectSandwichELISA.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
SampleImmunosorbentTime
The duration of Sample incubation.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
SampleImmunosorbent Temperature
The temperature of the Sample incubation.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
SampleImmunosorbentMixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Sample incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
SampleImmunosorbentWashVolume
The volume of WashBuffer added to rinse off the unbound primary antibody after Sample incubation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
SampleImmunosorbentNumberOf Washes
The number of rinses performed after Sample incubation.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
PrimaryAntibodyImmunosorbent Volumes
For each member of SamplesIn, the volume of the Primary Antibody to be loaded on the ELISAPlate for immunosordent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
PrimaryAntibodyImmunosorbent Time
The duration of Primary Antibody incubation.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
PrimaryAntibodyImmunosorbent Temperature
The temperature of the Primary Antibody incubation.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
PrimaryAntibodyImmunosorbent MixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Primary Antibody incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
PrimaryAntibodyImmunosorbent WashVolume
The volume of WashBuffer added to rinse off the unbound primary antibody after PrimaryAntibody incubation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
PrimaryAntibodyImmunosorbent NumberOfWashes
The number of rinses performed after PrimaryAntibody incubation.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
SecondaryAntibody ImmunosorbentVolumes
For each member of SamplesIn, the volume of the Secondary Antibody to be loaded on the ELISAPlate for immunosordent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
SecondaryAntibody ImmunosorbentTime
The duration of Secondary Antibody incubation.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
SecondaryAntibody ImmunosorbentTemperature
The temperature of the Secondary Antibody incubation.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
SecondaryAntibody ImmunosorbentMixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Secondary Antibody incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
SecondaryAntibody ImmunosorbentWashVolume
The volume of WashBuffer added to rinse off the unbound Secondary antibody after SecondaryAntibody incubation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[25 Microliter, 300 Microliter]
SecondaryAntibody ImmunosorbentNumberOfWashes
The number of rinses performed after SecondaryAntibody incubation.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0]
Detection
SubstrateSolutions
For each member of SamplesIn, defines the one-part substrate solution such as PNPP.
Format: Multiple
Class: Link
StopSolutions
For each member of SamplesIn, the reagent that is used to stop the reaction between the enzyme and its substrate.
Format: Multiple
Class: Link
SubstrateSolutionVolumes
For each member of SamplesIn, the volume of substrate to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StopSolutionVolumes
For each member of SamplesIn, the volume of StopSolution to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
SubstrateIncubationTime
The time during which the the colorimetric reaction occurs.
Format: Single
Class: Real
Unit: 1 hour
Programmatic Pattern: RangeP[0 Minute, 24 Hour]
SubstrateIncubation Temperature
The temperature of the Substrate incubation, in order for the detection reaction to take place where the antibody-conjugated enzyme (such as Horseradish Peroxidase or Alkaline Phosphatase) catalyzes the colorimetric reaction.
Format: Single
Class: Expression
Programmatic Pattern: Ambient | RangeP[4 Celsius, 50 Celsius]
SubstrateIncubationMixRate
The speed at which the plate is shaken (orbitally, at a radius of 2 mm) during Substrate incubation. Mixing is not recommended when incubation volume is higher than 200 Microliters, in which case the options should be set to Null.
Format: Single
Class: Real
Unit: 1 revolution per minute
Programmatic Pattern: RangeP[0 RPM, 1000 RPM]
PrereadAbsorbanceWavelengths
The wavelength used to detect the absorbance of light produced by colorimetric reaction before the sample is quenched by the StopSolutions.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
AbsorbanceWavelengths
The wavelength used to detect the absorbance of light by the product of the detection reaction.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
SignalCorrection
The absorbance reading that is used to eliminate the interference of background absorption (such as from ELISAPlate material and dust). If True, a reading at 620 nm is read at the same time of the AbsorbanceWavelength. The correction is done by subtracting the reading at 620nm from that at the AbsorbanceWavelength.
Format: Single
Class: Expression
Programmatic Pattern: Alternatives[BooleanP]
Standard
Standards
For each member of Standards, a sample containing known amount of TargetAntigen molecule. Standard is used for the quantification of Standard analyte.
Format: Multiple
Class: Link
StandardTargetAntigens
For each member of Standards, the Analyte molecule(e.g., peptide, protein, and hormone) detected and quantified in the samples by Antibodies in the ELISA experiment. This option is used to automatically set sample Antibodies and the corresponding experiment conditions of Standards and Blanks.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Model[Molecule]]
Relation: Model[Molecule]
StandardDilutionCurves
For each member of Standards, the multi-step dilutions of standards, presented as {Standard transfer volume, Diluent transfer volume}.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
StandardSerialDilutionCurves
For each member of Standards, the multi-step serial dilutions of standards, presented as {Transfer volume of previous dilution, Diluent transfer volume}.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Microliter], GreaterEqualP[0 Microliter]}..}
StandardDiluent
The buffer used to dilute Standards.
Format: Single
Class: Link
StandardStorageConditions
For each member of Standards, the condition under which the unused portion of Standardstock sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardCoatingAntibodies
For each member of Standards, the sample containing the antibody that is used in for coating in FastELISA.
Format: Multiple
Class: Link
StandardCoatingAntibody DilutionFactors
For each member of Standards, the ratio of dilution of StandardCoatingAntibody. StandardCoatingAntibody is diluted with CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardCoatingAntibody Volumes
For each member of Standards, the volume of Coating added into the corresponding well of the assay plate. CoatingAntibody is diluted with CoatingBuffer. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardCaptureAntibodies
For each member of Standards, the sample containing the antibody that is used to pull down the antigen from sample solution to the plate in DirectSandwichELISA, IndirectSandwichELISA, and FastELISA.
Format: Multiple
Class: Link
StandardCaptureAntibody DilutionFactors
For each member of Standards, the ratio of dilution of StandardCaptureAntibody. For DirectSandwichELISA and IndirectSandwichELISA, StandardCaptureAntibody is diluted with CoatingBuffer. For FastELISA, StandardCaptureAntibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardCaptureAntibody Volumes
For each member of Standards, the volume of CaptureAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardReferenceAntigens
For each member of Standards, the SAMPLE containing the antigen that is used in DirectCompetitiveELISA or InirectCompetitiveELISA. The StandardReferenceAntigen competes with sample antigen for the binding of the StandardPrimaryAntibody. Reference Antigen is sometimes also referred to as Inhibitor Antigen.
Format: Multiple
Class: Link
StandardReferenceAntigen DilutionFactors
For each member of Standards, the ratio of dilution of StandardReferenceAntigen. For DirectCompetitiveELISA and IndirectCompetitiveELISA, the StandardReferenceAntigenStandard is diluted in CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardReferenceAntigen Volumes
For each member of Standards, the volume of ReferenceAntigen added into the corresponding well of the assay plate. ReferenceAntigenVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardPrimaryAntibodies
For each member of Standards, the antibody that directly binds with the analyte.
Format: Multiple
Class: Link
StandardPrimaryAntibody DilutionFactors
For each member of Standards, the ratio of dilution of StandardPrimaryAntibody. For DirectELISA, IndirectELISA, DirectSandwichELISA, and IndirectSandwichELISA, the antibody is diluted with ImmunosorbentBuffer. For DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA, the antibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardPrimaryAntibody Volumes
For each member of Standards, the volume of PrimaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardSecondaryAntibodies
For each member of Standards, the antibody that binds to the primary antibody.
Format: Multiple
Class: Link
StandardSecondaryAntibody DilutionFactors
For each member of Standards, the ratio of dilution of StandardSecondaryAntibody. StandardSecondaryAntibody is always diluted in the ImmunosorbentBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
StandardSecondaryAntibody Volumes
For each member of Standards, the volume of SecondaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardCoatingVolumes
For each member of Standards, the amount of Standard that is aliquoted into the ELISAPlate, in order for the Standard to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardReferenceAntigen CoatingVolumes
For each member of Standards, the amount of ReferenceAntigen that is aliquoted into the assay plate, in order for the ReferenceAntigen to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardCoatingAntibody CoatingVolumes
For each member of Standards, the amount of diluted CoatingAntibody that is aliquoted into the ELISAPlate, in order for the CoatingAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardBlockingVolumes
For each member of Standards, the amount of BlockingBuffer that is aliquoted into the appropriate wells of the ELISAPlate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 300 Microliter]
StandardAntibodyComplex ImmunosorbentVolumes
For each member of Standards, the volume of the StandardAntibodyComplex to be loaded on the ELISAPlate. In DirectCompetitiveELISA and IndirectCompetitiveELISA, this step enables the free primary antibody to bind to the ReferenceAntigen coated on the plate. In FastELISA, this step enables the PrimaryAntibody-TargetAntigen-CaptureAntibody complex to bind to the CoatingAntibody on the plate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardImmunosorbentVolumes
For each member of Standards, the volume of the Standard to be loaded on the ELISAPlate for the target antigen to bind to the capture antibody in DirectSandwichELISA and IndirectSandwichELISA.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardPrimaryAntibody ImmunosorbentVolumes
For each member of Standards, the volume of the Primary Antibody to be loaded on the ELISAPlate for Immunosorbent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardSecondaryAntibody ImmunosorbentVolumes
For each member of Standards, the volume of the Secondary Antibody to be loaded on the ELISAPlate for immunosordent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardSubstrateSolutions
For each member of Standards, defines the one-part substrate solution such as PNPP.
Format: Multiple
Class: Link
StandardStopSolutions
For each member of Standards, the reagent that is used to stop the reacelisaMasterSwitchtion between the enzyme and its substrate.
Format: Multiple
Class: Link
StandardSubstrateSolution Volumes
For each member of Standards, the volume of substrate to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
StandardStopSolutionVolumes
For each member of Standards, the volume of StopSolution to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
Blank
Blanks
For each member of Blanks, a sample containing no TargetAntigen, used as a baseline or negative control for the ELISA.
Format: Multiple
Class: Link
BlankTargetAntigens
For each member of Blanks,The Analyte molecule(e.g., peptide, protein, and hormone) detected and quantified in the blanks by Antibodies in the ELISA experiment. This option is used to automatically set Antibodies and the corresponding experiment conditions.
Format: Multiple
Class: Link
Programmatic Pattern: ObjectP[Model[Molecule]]
Relation: Model[Molecule]
BlankStorageConditions
For each member of Blanks, the condition under which the unused portion of Blank sample should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
BlankCoatingAntibodies
For each member of Blanks,The sample containing the antibody that is used in for coating in FastELISA.
Format: Multiple
Class: Link
BlankCoatingAntibodyDilution Factors
For each member of Blanks,The ratio of dilution of BlankCoatingAntibody. BlankCoatingAntibody is diluted with CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
BlankCoatingAntibodyVolumes
For each member of Blanks,The volume of Coating added into the corresponding well of the assay plate. CoatingAntibody is diluted with CoatingBuffer. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankCaptureAntibodies
For each member of Blanks,The sample containing the antibody that is used to pull down the antigen from sample solution to the plate in DirectSandwichELISA, IndirectSandwichELISA, and FastELISA.
Format: Multiple
Class: Link
BlankCaptureAntibodyDilution Factors
For each member of Blanks,The ratio of dilution of BlankCaptureAntibody. For DirectSandwichELISA and IndirectSandwichELISA, BlankCaptureAntibody is diluted with CoatingBuffer. For FastELISA, BlankCaptureAntibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
BlankCaptureAntibodyVolumes
For each member of Blanks,The volume of CaptureAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankReferenceAntigens
For each member of Blanks, the SAMPLE containing the antigen that is used in DirectCompetitiveELISA or InirectCompetitiveELISA. The BlankReferenceAntigen competes with sample antigen for the binding of the BlankPrimaryAntibody. Reference Antigen is sometimes also referred to as Inhibitor Antigen.
Format: Multiple
Class: Link
BlankReferenceAntigenDilution Factors
For each member of Blanks, the ratio of dilution of BlankReferenceAntigen. For DirectCompetitiveELISA and IndirectCompetitiveELISA, the BlankReferenceAntigenBlank is diluted in CoatingBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
BlankReferenceAntigenVolumes
For each member of Blanks, the volume of ReferenceAntigen added into the corresponding well of the assay plate. ReferenceAntigenVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankPrimaryAntibodies
For each member of Blanks, the antibody that directly binds with the analyte.
Format: Multiple
Class: Link
BlankPrimaryAntibodyDilution Factors
For each member of Blanks, the ratio of dilution of BlankPrimaryAntibody. For DirectELISA, IndirectELISA, DirectSandwichELISA, and IndirectSandwichELISA, the antibody is diluted with ImmunosorbentBuffer. For DirectCompetitiveELISA, IndirectCompetitiveELISA, and FastELISA, the antibody is diluted in the corresponding sample. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
BlankPrimaryAntibodyVolumes
For each member of Blanks, the volume of PrimaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankSecondaryAntibodies
For each member of Blanks, the antibody that binds to the primary antibody.
Format: Multiple
Class: Link
BlankSecondaryAntibody DilutionFactors
For each member of Blanks, the ratio of dilution of BlankSecondaryAntibody. BlankSecondaryAntibody is always diluted in the ImmunosorbentBuffer. Either AssayConcentration or DilutionFactor should be provided but not both.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0, 1]
BlankSecondaryAntibodyVolumes
For each member of Blanks, the volume of SecondaryAntibody added into the corresponding well of the assay plate. AntibodyVolume is used as an alternative to AssayConcentration or DilutionFactor. During antibody preparation, a master mix will be made for antibody dilution, and the diluted Antibodies will be aliquoted into each well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankCoatingVolumes
For each member of Blanks, the amount of Blank that is aliquoted into the ELISAPlate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankReferenceAntigenCoating Volumes
For each member of Blanks, the amount of ReferenceAntigen that is aliquoted into the assay plate, in order for the ReferenceAntigen to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankCoatingAntibodyCoating Volumes
For each member of Blanks, the amount of diluted CoatingAntibody that is aliquoted into the ELISAPlate, in order for the CoatingAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankCaptureAntibodyCoating Volumes
For each member of Blanks, the amount of diluted CaptureAntibody that is aliquoted into the ELISAPlate, in order for the CaptureAntibody to be adsorbed to the surface of the well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankBlockingVolumes
For each member of Blanks, the amount of BlockingBuffer that is aliquoted into the appropriate wells of the ELISAPlate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 300 Microliter]
BlankAntibodyComplex ImmunosorbentVolumes
For each member of Blanks, the volume of the BlankAntibodyComplex to be loaded on the ELISAPlate. In DirectCompetitiveELISA and IndirectCompetitiveELISA, this step enables the free primary antibody to bind to the ReferenceAntigen coated on the plate. In FastELISA, this step enables the PrimaryAntibody-TargetAntigen-CaptureAntibody complex to bind to the CoatingAntibody on the plate.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankImmunosorbentVolumes
For each member of Blanks, the volume of the Blank to be loaded on the ELISAPlate for the target antigen to bind to the capture antibody in DirectSandwichELISA and IndirectSandwichELISA.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankPrimaryAntibody ImmunosorbentVolumes
For each member of Blanks, the volume of the Primary Antibody to be loaded on the ELISAPlate for Immunosorbent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankSecondaryAntibody ImmunosorbentVolumes
For each member of Blanks, the volume of the Secondary Antibody to be loaded on the ELISAPlate for immunosordent assay.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankSubstrateSolutions
For each member of Blanks, defines the one-part substrate solution such as PNPP.
Format: Multiple
Class: Link
BlankStopSolutions
For each member of Blanks, the reagent that is used to stop the reaction between the enzyme and its substrate.
Format: Multiple
Class: Link
BlankSubstrateSolutionVolumes
For each member of Blanks, the volume of substrate to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
BlankStopSolutionVolumes
For each member of Blanks, the volume of StopSolution to be added to the corresponding well.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: RangeP[0 Microliter, 200 Microliter]
Assay Plate
ELISAPlate
The assay plate each sample, standard, and blank will be loaded into and the immunosorbent assay will take place. This plate can be pre-coated and blocked in advance.
Format: Single
Class: Link
SecondaryELISAPlate
The second assay plate, if needed.
Format: Single
Class: Link
ELISAPlateAssignment
The arrangement of samples and their corresponding reagents in the ELISAPlate.
Format: Multiple
[Type]
Header: Type
Class: Expression
[Sample]
Header: Sample
Class: Link
[Spike]
Header: Spike
Class: Link
[SpikeDilutionFactor]
Header: SpikeDilutionFactor
Class: Real
[SampleDilutionFactors]
Header: SampleDilutionFactors
Class: Expression
[CoatingAntibody]
Header: CoatingAntibody
Class: Link
[CoatingAntibodyDilutionFactor]
Header: CoatingAntibodyDilutionFactor
Class: Real
[CaptureAntibody]
Header: CaptureAntibody
Class: Link
[CaptureAntibodyDilutionFactor]
Header: CaptureAntibodyDilutionFactor
Class: Real
[ReferenceAntigen]
Header: ReferenceAntigen
Class: Link
[ReferenceAntigenDilutionFactor]
Header: ReferenceAntigenDilutionFactor
Class: Real
[PrimaryAntibody]
Header: PrimaryAntibody
Class: Link
[PrimaryAntibodyDilutionFactor]
Header: PrimaryAntibodyDilutionFactor
Class: Real
[SecondaryAntibody]
Header: SecondaryAntibody
Class: Link
[SecondaryAntibodyDilutionFactor]
Header: SecondaryAntibodyDilutionFactor
Class: Real
[CoatingVolume]
Header: CoatingVolume
Class: Real
Unit: 1 microliter
[BlockingVolume]
Header: BlockingVolume
Class: Real
Unit: 1 microliter
[SampleAntibodyComplexImmunosorbentVolume]
Header: SampleAntibodyComplexImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SampleImmunosorbentVolume]
Header: SampleImmunosorbentVolume
Class: Real
Unit: 1 microliter
[PrimaryAntibodyImmunosorbentVolume]
Header: PrimaryAntibodyImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SecondaryAntibodyImmunosorbentVolume]
Header: SecondaryAntibodyImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SubstrateSolution]
Header: SubstrateSolution
Class: Link
[StopSolution]
Header: StopSolution
Class: Link
[SubstrateSolutionVolume]
Header: SubstrateSolutionVolume
Class: Real
Unit: 1 microliter
[StopSolutionVolume]
Header: StopSolutionVolume
Class: Real
Unit: 1 microliter
[Data]
Header: Data
Class: Link
Relation: Object[Data]
SecondaryELISAPlateAssignment
The arrangement of samples and their corresponding reagents in the SecondaryELISAPlate.
Format: Multiple
[Type]
Header: Type
Class: Expression
[Sample]
Header: Sample
Class: Link
[Spike]
Header: Spike
Class: Link
[SpikeDilutionFactor]
Header: SpikeDilutionFactor
Class: Real
[SampleDilutionFactors]
Header: SampleDilutionFactors
Class: Expression
[CoatingAntibody]
Header: CoatingAntibody
Class: Link
[CoatingAntibodyDilutionFactor]
Header: CoatingAntibodyDilutionFactor
Class: Real
[CaptureAntibody]
Header: CaptureAntibody
Class: Link
[CaptureAntibodyDilutionFactor]
Header: CaptureAntibodyDilutionFactor
Class: Real
[ReferenceAntigen]
Header: ReferenceAntigen
Class: Link
[ReferenceAntigenDilutionFactor]
Header: ReferenceAntigenDilutionFactor
Class: Real
[PrimaryAntibody]
Header: PrimaryAntibody
Class: Link
[PrimaryAntibodyDilutionFactor]
Header: PrimaryAntibodyDilutionFactor
Class: Real
[SecondaryAntibody]
Header: SecondaryAntibody
Class: Link
[SecondaryAntibodyDilutionFactor]
Header: SecondaryAntibodyDilutionFactor
Class: Real
[CoatingVolume]
Header: CoatingVolume
Class: Real
Unit: 1 microliter
[BlockingVolume]
Header: BlockingVolume
Class: Real
Unit: 1 microliter
[SampleAntibodyComplexImmunosorbentVolume]
Header: SampleAntibodyComplexImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SampleImmunosorbentVolume]
Header: SampleImmunosorbentVolume
Class: Real
Unit: 1 microliter
[PrimaryAntibodyImmunosorbentVolume]
Header: PrimaryAntibodyImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SecondaryAntibodyImmunosorbentVolume]
Header: SecondaryAntibodyImmunosorbentVolume
Class: Real
Unit: 1 microliter
[SubstrateSolution]
Header: SubstrateSolution
Class: Link
[StopSolution]
Header: StopSolution
Class: Link
[SubstrateSolutionVolume]
Header: SubstrateSolutionVolume
Class: Real
Unit: 1 microliter
[StopSolutionVolume]
Header: StopSolutionVolume
Class: Real
Unit: 1 microliter
[Data]
Header: Data
Class: Link
Relation: Object[Data]
Last modified on Mon 28 Nov 2022 2:02:19