Object[Protocol, HPLC]
A protocol describing the separation of samples using high performance liquid chromatography.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, HPLC, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, HPLC]
Pattern Description: Object[Protocol, HPLC]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The device containing a pump, column oven, detector(s), and possible fraction collector that executes this experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Scale
Indicates if the HPLC is intended to separate sample (Preparative) and therefore collect fractions and/or analyze properties of the sample (Analytical).
Format: Single
Class: Expression
Programmatic Pattern: PurificationScaleP
SeparationMode
The type of chromatographic separation that categorizes the mobile and stationary phase used, ideally for optimal sample separation and resolution.
Format: Single
Class: Expression
Programmatic Pattern: SeparationModeP
Detectors
The types of devices on the instrument that are used to perform measurements on the sample.
Format: Multiple
Class: Expression
Programmatic Pattern: ChromatographyDetectorTypeP
PurgeSyringe
The syringe used to pull air and buffer from the buffer lines to clear them of air bubbles.
Format: Single
Class: Link
Programmatic Pattern: _Link
PurgeWasteContainer
The container used to hold waste from air bubble removal procedures for buffer lines. It is also used to hold waste from rinsing plumbing connections.
Format: Single
Class: Link
Programmatic Pattern: _Link
ColumnSelection
The configurations of stationary phase devices used for analyte separation.
Format: Multiple
[Column]
Header: Column
Class: Link
[SecondaryColumn]
Header: SecondaryColumn
Class: Link
[TertiaryColumn]
Header: TertiaryColumn
Class: Link
IncubateColumn
Indicates if the columns are placed inside the column oven compartment of the HPLC instrument during the experiment.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
BufferA
The solvent pumped through channel A of the flow path.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferB
The solvent pumped through channel B of the flow path.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferC
The solvent pumped through channel C of the flow path.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferD
The solvent pumped through channel D of the flow path.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferACap
The cap used to aspirate BufferA during this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferBCap
The cap used to aspirate BufferB during this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferCCap
The cap used to aspirate BufferC during this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDCap
The cap used to aspirate BufferD during this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
InitialBufferAVolume
The volume of BufferA immediately before the experiment is started.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferBVolume
The volume of BufferB immediately before the experiment is started.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferCVolume
The volume of BufferC immediately before the experiment is started.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferDVolume
The volume of BufferD immediately before the experiment is started.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferAAppearance
The image of BufferA taken immediately before the experiment is started.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InitialBufferBAppearance
The image of BufferB taken immediately before the experiment is started.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InitialBufferCAppearance
The image of BufferC taken immediately before the experiment is started.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InitialBufferDAppearance
The image of BufferD taken immediately before the experiment is started.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FlowRate
For each member of SamplesIn, the total rate of mobile phase pumped through the instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
MaxAcceleration
The maximum rate at which it is safe to increase the flow rate for the column and instrument during the run.
Format: Single
Class: Real
Unit: 1 milliliter per minute squared
Programmatic Pattern: 0 Milli Liter
-------------
Minute
Hold[GreaterEqualP[-------------]]
Minute
InjectionTable
The sequence of samples injected for a given experiment run, including for ColumnPrime, SamplesIn, Standards, Blanks, and ColumnFlush.
Format: Multiple
[Type]
Header: Type
Class: Expression
[Sample]
Header: Sample
Class: Link
[InjectionVolume]
Header: InjectionVolume
Class: Real
Unit: 1 microliter
[Column]
Header: Column
Class: Link
[Gradient]
Header: Gradient
Class: Link
Relation: Object[Method]
[DilutionFactor]
Header: DilutionFactor
Class: Real
[ColumnTemperature]
Header: ColumnTemperature
Class: Real
Unit: 1 degree Celsius
[Data]
Header: Data
Class: Link
Relation: Object[Data]
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
SampleTemperature
The nominal temperature of the chamber input samples are incubated in prior to injection into the column.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
SampleVolumes
For each member of SamplesIn, the volume taken from the sample and injected into the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
PlateSeal
The package of piercable, adhesive film used to cover the plates containing the samples to be injected in order to mitigate sample evaporation.
Format: Single
Class: Link
Programmatic Pattern: _Link
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
StandardData
The chromatography traces generated for the standard's injection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
BlankData
The chromatography traces generated for the blank's injection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
PrimeData
The chromatography traces generated for any column prime runs.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
FlushData
The chromatography traces generated for any column flush runs.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialAnalyteVolumes
The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) measured prior to injection.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalAnalyteVolumes
The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) measured at the end of the protocol.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter]
InjectedAnalyteVolumes
The volume of each analyte sample (SamplesIn if drawn directly or AliquotSamples if instructed to aliquot prior to the assay) that is injected during the protocol, calculated as the difference between InitialAnalyteVolumes and FinalAnalyteVolumes.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Liter]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
TubingRinseSolution
The solution used to rinse buffers lines before and after and the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
NeedleWashSolution
The solution used to wash the injection needle and pumps before, during, and after the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
CapWashAdapter
Buffer cap plumbing adapter used to clean the tubing interior of used buffer caps.
Format: Single
Class: Link
Programmatic Pattern: _Link
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ReadyCheckComputations
The list of manifold job notebooks that was used to evaluate ReadyCheck for this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Job]
Column Installation
Columns
For each member of SamplesIn, the stationary phase device through which the Buffers and input samples flow. It adsorbs and separates the molecules within the sample based on the properties of the Buffers, samples, column material, and ColumnTemperature.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SecondaryColumns
For each member of SamplesIn, the second stationary phase device through which the Buffers and input samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
TertiaryColumns
For each member of SamplesIn, the third stationary phase device through which the Buffers and input samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ColumnOrientation
For each member of ColumnSelection, the direction of the column for the protocol with respect to the flow.
Format: Multiple
Class: Expression
Programmatic Pattern: ColumnOrientationP
ColumnJoins
The connections used to link multiple columns.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GuardColumn
For each member of ColumnSelection, the protective device placed in the flow path before the Column in order to adsorb fouling contaminants and, thus, preserve the Column lifetime. If GuardColumnOrientation is Reverse, the GuardColumn is placed after Columns in the flow path.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GuardCartridge
For each member of ColumnSelection, the module that holds the adsorbent within the GuardColumn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ResinLoading
For each member of ColumnSelection, the amount (in grams) of adsorbent that is loaded into the GuardColumn.
Format: Multiple
Class: Real
Unit: 1 gram
Programmatic Pattern: GreaterP[0 Gram]
GuardColumnJoin
The connection used to link the column and guard column employed in this HPLC purification.
Format: Single
Class: Link
Programmatic Pattern: _Link
GuardColumnOrientation
For each member of ColumnSelection, the direction of the GuardColumn for the protocol. If a Column is used and GuardColumnOrientation is Reverse, then the GuardColumn is placed after the column relative to the direction of the flow path.
Format: Multiple
Class: Expression
Programmatic Pattern: ColumnOrientationP
Column Prime
ColumnPrimeGradients
For each member of ColumnSelection, the composition of the buffers within the flow, defined for specific time points during the equilibration of the Columns (column prime).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
ColumnPrimeGradientAs
For each member of ColumnSelection, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run for each column prime run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientBs
For each member of ColumnSelection, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run for each column prime run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientCs
For each member of ColumnSelection, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for the entire run for each column prime run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientDs
For each member of ColumnSelection, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for the entire run for each column prime run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeFlowRates
For each member of ColumnSelection, the total rate of mobile phase pumped through the instrument for each column prime.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
ColumnPrimeTemperatures
For each member of ColumnSelection, the nominal temperature of the column compartment for each column prime run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnPrimeAbsorbance Wavelengths
For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for UVVis detectors for each column prime run.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnPrimeMinAbsorbance Wavelengths
For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector for each column prime run.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnPrimeMaxAbsorbance Wavelengths
For each member of ColumnSelection, the maximum wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector for each column prime run.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnPrimeWavelength Resolutions
For each member of ColumnSelection, the increment in wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector for each column prime run.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnPrimeUVFilters
For each member of ColumnSelection, indicates if UV wavelengths (less than 210 nm) is blocked from being transmitted through the sample for Absorbance detectors (UVVis or PhotoDiodeArray) for each column prime run.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnPrimeAbsorbanceSampling Rates
For each member of ColumnSelection, the frequency of measurement for UVVis or Photo Diode Array (PDA) detectors for each column prime run.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
ColumnPrimeExcitation Wavelengths
For each member of ColumnSelection, the primary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeSecondary ExcitationWavelengths
For each member of ColumnSelection, the secondary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeTertiaryExcitation Wavelengths
For each member of ColumnSelection, the tertiary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeQuaternary ExcitationWavelengths
For each member of ColumnSelection, the quaternary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeEmission Wavelengths
For each member of ColumnSelection, the primary monochromator/filter wavelength used to filter emitted light in the fluorescence flow cell during column prime before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeSecondaryEmission Wavelengths
For each member of ColumnSelection, the secondary monochromator/filter wavelength used to filter emitted light in the fluorescence flow cell during column prime before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeTertiaryEmission Wavelengths
For each member of ColumnSelection, the tertiary monochromator/filter wavelength used to filter emitted light in the fluorescence flow cell during column prime before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeQuaternaryEmission Wavelengths
For each member of ColumnSelection, the quaternary monochromator/filter wavelength used to filter emitted light in the fluorescence flow cell during column prime before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeEmissionCutOff Filters
For each member of ColumnSelection, the cut-off wavelength(s) to pre-select the emitted light from the flow downstream of the column and allow only the light with wavelength above the desired value to pass before the light enters emission monochromator for final wavelength selection during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeFluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnPrimeExcitationWavelengths/ColumnPrimeEmissionWavelengths channel during column prime on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnPrimeSecondary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnPrimeSecondaryExcitationWavelengths/ColumnPrimeSecondaryEmissionWavelengths channel during column prime on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnPrimeTertiary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnPrimeTertiaryExcitationWavelengths/ColumnPrimeTertiaryEmissionWavelengths channel during column prime on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnPrimeQuaternary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnPrimeQuaternaryExcitationWavelengths/ColumnPrimeQuaternaryEmissionWavelengths channel during column prime on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnPrimeFluorescence SamplingRates
For each member of ColumnSelection, the frequency of fluorescence measurement during column prime.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterEqualP[0 ------]]
Second
ColumnPrimeMultiChannel FluorescenceSamplingRates
For each member of ColumnSelection, the frequency of fluorescence measurement during column prime when multiple fluorescence measurement channels are selected.
Format: Multiple
Class: Expression
Programmatic Pattern: HPLCFluorescenceSamplingRateP
ColumnPrimeFluorescenceFlow CellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the fluorescence flow cell of the fluorescence detector during column prime.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnPrimeLightScattering LaserPowers
For each member of ColumnSelection, the laser power used in the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) detector during column prime.
Format: Multiple
Class: Real
Unit: 1 percent
Programmatic Pattern: RangeP[10 Percent, 100 Percent]
ColumnPrimeLightScattering FlowCellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) flow cell during column prime.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnPrimeRefractiveIndex Methods
For each member of ColumnSelection, the type of refractive index measurement for the refractive index (RI) detector during column prime.
Format: Multiple
Class: Expression
Programmatic Pattern: RefractiveIndex | DifferentialRefractiveIndex
ColumnPrimeRefractiveIndex FlowCellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the refractive index flow cell of the refractive index (RI) detector during column prime.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnPrimeCircularDichroism Wavelengths
For each member of ColumnSelection, the wavelength of the light used for circular dichroism measurement during column prime.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnPrimeCircularDichroism SamplingRates
For each member of ColumnSelection, the frequency of circular dichroism measurement during column prime.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
ColumnPrimeNebulizerGases
For each member of ColumnSelection, indicates if sheath gas is turned on for the Evaporative Light Scattering Detector (ELSD) for each column prime run.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnPrimeNebulizerGas Pressures
For each member of ColumnSelection, if NebulizerGas is True, the applied pressure to the sheath gas for each column prime run.
Format: Multiple
Class: Real
Unit: 1 pound‐force per inch squared
Programmatic Pattern: RangeP[20 PSI, 60 PSI, 1 PSI]
ColumnPrimeNebulizerGas Heatings
For each member of ColumnSelection, if NebulizerGas is True, indicates if sheath gas is heated or not for the Evaporative Light Scattering Detector (ELSD) for each column prime run.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnPrimeNebulizerHeating Powers
For each member of ColumnSelection, the relative magnitude of the heating element used to heat the sheath gas for each column prime run.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0 Percent, 100 Percent]
ColumnPrimeDriftTube Temperatures
For each member of ColumnSelection, the temperature of the chamber through which the sprayed analyte travels for each column prime run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
ColumnPrimeELSDGains
For each member of ColumnSelection, the signal amplification of the Evaporative Light Scattering Detector (ELSD) measurement for each column prime run.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0.01, 1000., 0.01]
ColumnPrimeELSDSamplingRates
For each member of ColumnSelection, the frequency of measurement for the Evaporative Light Scattering Detector (ELSD) for each column prime run.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
Gradient
GradientA
For each member of SamplesIn, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientB
For each member of SamplesIn, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientC
For each member of SamplesIn, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientD
For each member of SamplesIn, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
Gradients
For each member of SamplesIn, the buffer gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
ColumnTemperatures
For each member of SamplesIn, the nominal temperature of the column compartment during a run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
FinalBufferAVolume
The volume of BufferA immediately after the experiment is completed.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferBVolume
The volume of BufferB immediately after the experiment is completed.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferCVolume
The volume of BufferC immediately after the experiment is completed.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferDVolume
The volume of BufferD immediately after the experiment is completed.
Format: Single
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferAAppearance
The image of BufferA taken immediately after the experiment is completed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FinalBufferBAppearance
The image of BufferB taken immediately after the experiment is completed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FinalBufferCAppearance
The image of BufferC taken immediately after the experiment is completed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FinalBufferDAppearance
The image of BufferD taken immediately after the experiment is completed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Detection
AbsorbanceWavelength
For each member of SamplesIn, the wavelength of light absorbed in the detector's flow cell for UVVis detectors.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
MinAbsorbanceWavelength
For each member of SamplesIn, the wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
MaxAbsorbanceWavelength
For each member of SamplesIn, the maximum wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
WavelengthResolution
For each member of SamplesIn, the increment in wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
UVFilter
For each member of SamplesIn, indicates if UV wavelengths (less than 210 nm) is blocked from being transmitted through the sample for Absorbance detectors (UVVis or PhotoDiodeArray).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
AbsorbanceSamplingRate
For each member of SamplesIn, the frequency of measurement for UVVis or Photo Diode Array (PDA) detectors.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
ExcitationWavelengths
For each member of SamplesIn, the primary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
SecondaryExcitation Wavelengths
For each member of SamplesIn, the secondary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
TertiaryExcitationWavelengths
For each member of SamplesIn, the v monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
QuaternaryExcitation Wavelengths
For each member of SamplesIn, the quaternary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
EmissionWavelengths
For each member of SamplesIn, the primary monochromator/filter wavelength used to filter emitted light from the sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
SecondaryEmissionWavelengths
For each member of SamplesIn, the secondary monochromator/filter wavelength used to filter emitted light from the sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
TertiaryEmissionWavelengths
For each member of SamplesIn, the tertiary monochromator/filter wavelength used to filter emitted light from the sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
QuaternaryEmissionWavelengths
For each member of SamplesIn, the quaternary monochromator/filter wavelength used to filter emitted light from the sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
EmissionCutOffFilters
For each member of SamplesIn, the cut-off wavelength(s) to pre-select the emitted light from the sample and allow only the light with wavelength above the desired value to pass before the light enters emission monochromator for final wavelength selection.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
FluorescenceGains
For each member of SamplesIn, the signal amplification of ExcitationWavelengths/EmissionWavelengths channel for the sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
SecondaryFluorescenceGains
For each member of SamplesIn, the signal amplification of SecondaryExcitationWavelengths/SecondaryEmissionWavelengths channel for the sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
TertiaryFluorescenceGains
For each member of SamplesIn, the signal amplification of TertiaryExcitationWavelengths/TertiaryEmissionWavelengths channel for the sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
QuaternaryFluorescenceGains
For each member of SamplesIn, the signal amplification of QuaternaryExcitationWavelengths/QuaternaryEmissionWavelengths channel for the sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
FluorescenceSamplingRates
For each member of SamplesIn, the frequency of fluorescence measurement for the sample.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterEqualP[0 ------]]
Second
MultiChannelFluorescence SamplingRates
For each member of SamplesIn, the frequency of fluorescence measurement for the sample when multiple fluorescence measurement channels are selected.
Format: Multiple
Class: Expression
Programmatic Pattern: HPLCFluorescenceSamplingRateP
FluorescenceFlowCell Temperatures
For each member of SamplesIn, the nominal temperature setting inside the fluorescence flow cell of the fluorescence detector during the fluorescence measurement of the sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
LightScatteringLaserPowers
For each member of SamplesIn, the laser power used in the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) detector for the measurement of the sample.
Format: Multiple
Class: Real
Unit: 1 percent
Programmatic Pattern: RangeP[10 Percent, 100 Percent]
LightScatteringFlowCell Temperatures
For each member of SamplesIn, the nominal temperature setting inside the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) flow cell during the MALS and/or DLS measurement of the sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
RefractiveIndexMethods
For each member of SamplesIn, the type of refractive index measurement for the sample on the refractive index (RI) detector.
Format: Multiple
Class: Expression
Programmatic Pattern: RefractiveIndex | DifferentialRefractiveIndex
RefractiveIndexFlowCell Temperatures
For each member of SamplesIn, the nominal temperature setting inside the refractive index flow cell of the refractive index (RI) detector during the refractive index measurement of the sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
CircularDichroismWavelengths
For each member of SamplesIn, the wavelength of the light used for circular dichroism measurement of the sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
CircularDichroismSampling Rates
For each member of SamplesIn, the frequency of circular dichroism measurement for the sample.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
pHCalibration
Indicates if 2-point calibration of the pH probe is performed before the experiment starts.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
LowpHCalibrationBuffer
The low pH reference buffer used to calibrate the pH probe in the 2-point calibration.
Format: Single
Class: Link
HighpHCalibrationBuffer
The high pH reference buffer used to calibrate the pH probe in the 2-point calibration.
Format: Single
Class: Link
LowpHCalibrationTarget
The pH of the LowpHCalibrationBuffer that used to calibrate the pH probe in the 2-point calibration.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 14]
HighpHCalibrationTarget
The pH of the HighpHCalibrationBuffer that used to calibrate the pH probe in the 2-point calibration.
Format: Single
Class: Real
Programmatic Pattern: RangeP[0, 14]
pHTemperatureCompensation
Indicates if the measured pH value is automatically corrected according to the temperature inside the pH flow cell.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ConductivityCalibration
Indicates if 1-point calibration of the conductivity probe is performed before the experiment starts.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ConductivityCalibrationBuffer
The buffer that is used to calibrate the conductivity probe in the 1-point calibration.
Format: Single
Class: Link
ConductivityCalibrationTarget
The conductivity value of the ConductivityCalibrationBuffer used to calibrate the conductivity probe in the 1-point calibration.
Format: Single
Class: Real
Unit: 1 millisiemens per centimeter
Programmatic Pattern: Siemens
Hold[GreaterEqualP[0 Milli ----------]]
Centimeter
ConductivityTemperature Compensation
Indicates if the measured conductivity value is automatically corrected according to the temperature inside the conductivity flow cell.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
NebulizerGas
For each member of SamplesIn, indicates if sheath gas is turned on for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
NebulizerGasPressure
For each member of SamplesIn, if NebulizerGas is True, the applied pressure to the sheath gas.
Format: Multiple
Class: Real
Unit: 1 pound‐force per inch squared
Programmatic Pattern: RangeP[20 PSI, 60 PSI, 1 PSI]
NebulizerGasHeating
For each member of SamplesIn, if NebulizerGas is True, indicates if sheath gas is heated or not for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
NebulizerHeatingPower
For each member of SamplesIn, the relative magnitude of the heating element used to heat the sheath gas.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0 Percent, 100 Percent]
DriftTubeTemperature
For each member of SamplesIn, the temperature of the chamber through which the sprayed analyte travels.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
ELSDGain
For each member of SamplesIn, the signal amplification of the Evaporative Light Scattering Detector (ELSD) measurement.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0.01, 1000., 0.01]
ELSDSamplingRate
For each member of SamplesIn, the frequency of measurement for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
Standards
Standards
The samples with known properties used to calibrate peak integrations and retention times for a given run.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardSampleVolumes
For each member of Standards, the volume taken from the standard and injected onto the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
StandardColumns
For each member of Standards, the stationary phase device through which the Buffers and samples flow. It adsorbs and separates the molecules within the sample based on the properties of the Buffers, samples, column material, and ColumnTemperature..
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardSecondaryColumns
For each member of Standards, the second stationary phase device through which the Buffers and samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardTertiaryColumns
For each member of Standards, the third stationary phase device through which the Buffers and samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardGradients
For each member of Standards, the method that describes the gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
StandardGradientA
For each member of Standards, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientB
For each member of Standards, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientC
For each member of Standards, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientD
For each member of Standards, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardFlowRates
For each member of Standards, the total rate of mobile phase pumped through the instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
StandardColumnTemperatures
For each member of Standards, the nominal temperature of the column compartment during a run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardAbsorbanceWavelength
For each member of Standards, the wavelength of light absorbed in the detector's flow cell for UVVis detectors.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
StandardMinAbsorbance Wavelength
For each member of Standards, the wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
StandardMaxAbsorbance Wavelength
For each member of Standards, the maximum wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
StandardWavelengthResolution
For each member of Standards, the increment in wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
StandardUVFilter
For each member of Standards, indicates if UV wavelengths (less than 210 nm) is locked from being transmitted through the sample for Absorbance detectors (UVVis or PhotoDiodeArray).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardAbsorbanceSampling Rate
For each member of Standards, the frequency of measurement for UVVis or Photo Diode Array (PDA) detectors.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
StandardExcitationWavelengths
For each member of Standards, the primary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the standard sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardSecondaryExcitation Wavelengths
For each member of Standards, the secondary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the standard sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardTertiaryExcitation Wavelengths
For each member of Standards, the tertiary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the standard sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardQuaternaryExcitation Wavelengths
For each member of Standards, the quaternary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the standard sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardEmissionWavelengths
For each member of Standards, the primary monochromator/filter wavelength used to filter emitted light from the standard sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardSecondaryEmission Wavelengths
For each member of Standards, the secondary monochromator/filter wavelength used to filter emitted light from the standard sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardTertiaryEmission Wavelengths
For each member of Standards, the tertiary monochromator/filter wavelength used to filter emitted light from the standard sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardQuaternaryEmission Wavelengths
For each member of Standards, the quaternary monochromator/filter wavelength used to filter emitted light from the standard sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardEmissionCutOffFilters
For each member of Standards, the cut-off wavelength(s) to pre-select the emitted light from the sample and allow only the light with wavelength above the desired value to pass before the light enters emission monochromator for final wavelength selection.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardFluorescenceGains
For each member of Standards, the signal amplification of StandardExcitationWavelengths/StandardEmissionWavelengths channel for the standard sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
StandardSecondaryFluorescence Gains
For each member of Standards, the signal amplification of StandardSecondaryExcitationWavelengths/StandardSecondaryEmissionWavelengths channel for the standard sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
StandardTertiaryFluorescence Gains
For each member of Standards, the signal amplification of StandardTertiaryExcitationWavelengths/StandardTertiaryEmissionWavelengths channel for the standard sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
StandardQuaternary FluorescenceGains
For each member of Standards, the signal amplification of StandardQuaternaryExcitationWavelengths/StandardQuaternaryEmissionWavelengths channel for the standard sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
StandardFluorescenceSampling Rates
For each member of Standards, the frequency of fluorescence measurement for the standard sample.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterEqualP[0 ------]]
Second
StandardMultiChannel FluorescenceSamplingRates
For each member of Standards, the frequency of fluorescence measurement for the standard sample when multiple fluorescence measurement channels are selected.
Format: Multiple
Class: Expression
Programmatic Pattern: HPLCFluorescenceSamplingRateP
StandardFluorescenceFlowCell Temperatures
For each member of Standards, the nominal temperature setting inside the fluorescence flow cell of the fluorescence detector during the fluorescence measurement of the standard sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardLightScatteringLaser Powers
For each member of Standards, the laser power used in the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) detector for the measurement of the standard sample.
Format: Multiple
Class: Real
Unit: 1 percent
Programmatic Pattern: RangeP[10 Percent, 100 Percent]
StandardLightScatteringFlow CellTemperatures
For each member of Standards, the nominal temperature setting inside the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) flow cell during the MALS and/or DLS measurement of the standard sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardRefractiveIndex Methods
For each member of Standards, the type of refractive index measurement for the standard sample on the refractive index (RI) detector.
Format: Multiple
Class: Expression
Programmatic Pattern: RefractiveIndex | DifferentialRefractiveIndex
StandardRefractiveIndexFlow CellTemperatures
For each member of Standards, the nominal temperature setting inside the refractive index flow cell of the refractive index (RI) detector during the refractive index measurement of the standard sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
StandardCircularDichroism Wavelengths
For each member of Standards, the wavelength of the light used for circular dichroism measurement of the standard sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
StandardCircularDichroism SamplingRates
For each member of Standards, the frequency of circular dichroism measurement for the standard sample.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
StandardNebulizerGas
For each member of Standards, indicates if sheath gas is turned on for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardNebulizerGasPressure
For each member of Standards, if NebulizerGas is True, the applied pressure to the sheath gas.
Format: Multiple
Class: Real
Unit: 1 pound‐force per inch squared
Programmatic Pattern: RangeP[20 PSI, 60 PSI, 1 PSI]
StandardNebulizerGasHeating
For each member of Standards, if NebulizerGas is True, indicates if sheath gas is heated or not for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
StandardNebulizerHeatingPower
For each member of Standards, the relative magnitude of the heating element used to heat the sheath gas.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0 Percent, 100 Percent]
StandardDriftTubeTemperature
For each member of Standards, the temperature of the chamber through which the sprayed analyte travels.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
StandardELSDGain
For each member of Standards, the signal amplification of the Evaporative Light Scattering Detector (ELSD) measurement.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0.01, 1000., 0.01]
StandardELSDSamplingRate
For each member of Standards, the frequency of measurement for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
StandardsStorageConditions
For each member of Standards, the storage conditions under which the standard samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Blanking
Blanks
The samples with known properties used to calibrate background signal.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankSampleVolumes
For each member of Blanks, the volume taken from the blank and injected onto the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
BlankColumns
For each member of Blanks, the stationary phase device through which the Buffers and samples flow. It adsorbs and separates the molecules within the sample based on the properties of the Buffers, samples, column material, and ColumnTemperature.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankSecondaryColumns
For each member of Blanks, the second stationary phase device through which the Buffers and samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankTertiaryColumns
For each member of Blanks, the third stationary phase device through which the Buffers and samples flow.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankGradients
For each member of Blanks, the method that describes the gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
BlankGradientA
For each member of Blanks, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientB
For each member of Blanks, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientC
For each member of Blanks, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientD
For each member of Blanks, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankFlowRates
For each member of Blanks, the total rate of mobile phase pumped through the instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
BlankColumnTemperatures
For each member of Blanks, the nominal temperature of the column compartment during a run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
BlankAbsorbanceWavelength
For each member of Blanks, the wavelength of light absorbed in the detector's flow cell for UVVis detectors.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
BlankMinAbsorbanceWavelength
For each member of Blanks, the wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
BlankMaxAbsorbanceWavelength
For each member of Blanks, the maximum wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
BlankWavelengthResolution
For each member of Blanks, the increment in wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
BlankUVFilter
For each member of Blanks, indicates if UV wavelengths (less than 210 nm) is blocked from being transmitted through the sample for Absorbance detectors (UVVis or PhotoDiodeArray).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
BlankAbsorbanceSamplingRate
For each member of Blanks, the frequency of measurement for UVVis or Photo Diode Array (PDA) detectors.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
BlankExcitationWavelengths
For each member of Blanks, the primary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the blank sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankSecondaryExcitation Wavelengths
For each member of Blanks, the secondary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the blank sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankTertiaryExcitation Wavelengths
For each member of Blanks, the tertiary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the blank sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankQuaternaryExcitation Wavelengths
For each member of Blanks, the quaternary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds in the blank sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankEmissionWavelengths
For each member of Blanks, the primary monochromator/filter wavelength used to filter emitted light from the blank sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankSecondaryEmission Wavelengths
For each member of Blanks, the secondary monochromator/filter wavelength used to filter emitted light from the blank sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankTertiaryEmission Wavelengths
For each member of Blanks, the tertiary monochromator/filter wavelength used to filter emitted light from the blank sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankQuaternaryEmission Wavelengths
For each member of Blanks, the quaternary monochromator/filter wavelength used to filter emitted light from the blank sample inside the fluorescence flow cell before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankEmissionCutOffFilters
For each member of Blanks, the cut-off wavelength(s) to pre-select the emitted light from the sample and allow only the light with wavelength above the desired value to pass before the light enters emission monochromator for final wavelength selection.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankFluorescenceGains
For each member of Blanks, the signal amplification of BlankExcitationWavelengths/BlankEmissionWavelengths channel for the blank sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BlankSecondaryFluorescence Gains
For each member of Blanks, the signal amplification of BlankSecondaryExcitationWavelengths/BlankSecondaryEmissionWavelengths channel for the blank sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BlankTertiaryFluorescence Gains
For each member of Blanks, the signal amplification of BlankTertiaryExcitationWavelengths/BlankTertiaryEmissionWavelengths channel for the blank sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BlankQuaternaryFluorescence Gains
For each member of Blanks, the signal amplification of BlankQuaternaryExcitationWavelengths/BlankQuaternaryEmissionWavelengths channel for the blank sample on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BlankFluorescenceSampling Rates
For each member of Blanks, the frequency of fluorescence measurement for the blank sample.
Format: Multiple
Class: Expression
Programmatic Pattern: 1
Hold[GreaterEqualP[0 ------]]
Second
BlankMultiChannelFluorescence SamplingRates
For each member of Blanks, the frequency of fluorescence measurement for the blank sample when multiple fluorescence measurement channels are selected.
Format: Multiple
Class: Expression
Programmatic Pattern: HPLCFluorescenceSamplingRateP
BlankFluorescenceFlowCell Temperatures
For each member of Blanks, the nominal temperature setting inside the fluorescence flow cell of the fluorescence detector during the fluorescence measurement of the blank sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
BlankLightScatteringLaser Powers
For each member of Blanks, the laser power used in the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) detector for the measurement of the blank sample.
Format: Multiple
Class: Real
Unit: 1 percent
Programmatic Pattern: RangeP[10 Percent, 100 Percent]
BlankLightScatteringFlowCell Temperatures
For each member of Blanks, the nominal temperature setting inside the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) flow cell during the MALS and/or DLS measurement of the blank sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
BlankRefractiveIndexMethods
For each member of Blanks, the type of refractive index measurement for the blank sample on the refractive index (RI) detector.
Format: Multiple
Class: Expression
Programmatic Pattern: RefractiveIndex | DifferentialRefractiveIndex
BlankRefractiveIndexFlowCell Temperatures
For each member of Blanks, the nomial temperature setting inside the refractive index flow cell of the refractive index (RI) detector during the refractive index measurement of the blank sample.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
BlankCircularDichroism Wavelengths
For each member of Blanks, the wavelength of the light used for circular dichroism measurement of the blank sample.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
BlankCircularDichroism SamplingRates
For each member of Blanks, the frequency of circular dichroism measurement for the blank sample.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
BlankNebulizerGas
For each member of Blanks, indicates if sheath gas is turned on for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
BlankNebulizerGasPressure
For each member of Blanks, if NebulizerGas is True, the applied pressure to the sheath gas.
Format: Multiple
Class: Real
Unit: 1 pound‐force per inch squared
Programmatic Pattern: RangeP[20 PSI, 60 PSI, 1 PSI]
BlankNebulizerGasHeating
For each member of Blanks, if NebulizerGas is True, indicates if sheath gas is heated or not for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
BlankNebulizerHeatingPower
For each member of Blanks, the relative magnitude of the heating element used to heat the sheath gas.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0 Percent, 100 Percent]
BlankDriftTubeTemperature
For each member of Blanks, the temperature of the chamber through which the sprayed analyte travels.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
BlankELSDGain
For each member of Blanks, the signal amplification of the Evaporative Light Scattering Detector (ELSD) measurement.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0.01, 1000., 0.01]
BlankELSDSamplingRate
For each member of Blanks, the frequency of measurement for the Evaporative Light Scattering Detector (ELSD).
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
BlanksStorageConditions
For each member of Blanks, the storage conditions under which the blank samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Fraction Collection
FractionCollection
Indicates if column effluent is collected and separated.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
FractionCollectionDetector
The type of measurement that is used as signal to trigger fraction collection.
Format: Single
Class: Expression
Programmatic Pattern: ChromatographyDetectorTypeP
FractionCollectionMethods
For each member of SamplesIn, the collection parameters used for any samples that require fraction collection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
FractionContainers
The fraction containers that collected samples during the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Column Flush
ColumnFlushGradients
For each member of ColumnSelection, the method that describes the gradient used for each column flush (when solvent is run through without injection).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
ColumnFlushGradientAs
For each member of ColumnSelection, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for each column flush run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientBs
For each member of ColumnSelection, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for each column flush run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientCs
For each member of ColumnSelection, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for each column flush run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientDs
For each member of ColumnSelection, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for each column flush run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushFlowRates
For each member of ColumnSelection, the total rate of mobile phase pumped through the instrument for each column flush.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
ColumnFlushTemperatures
For each member of ColumnSelection, the nominal temperature of the column compartment for each column flush run.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnFlushAbsorbance Wavelengths
For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for UVVis detectors during the column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnFlushMinAbsorbance Wavelengths
For each member of ColumnSelection, the wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector during the column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnFlushMaxAbsorbance Wavelengths
For each member of ColumnSelection, the maximum wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector during the column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnFlushWavelength Resolutions
For each member of ColumnSelection, the increment in wavelength of light absorbed in the detector's flow cell for a Photo Diode Array (PDA) detector during the column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Centimeter]
ColumnFlushUVFilters
For each member of ColumnSelection, indicates if UV wavelengths (less than 210 nm) is blocked from being transmitted through the sample for Absorbance detectors (UVVis or PhotoDiodeArray) during the column flush.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnFlushAbsorbanceSampling Rates
For each member of ColumnSelection, the frequency of measurement for UVVis or Photo Diode Array (PDA) detectors during the column flush.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
ColumnFlushExcitation Wavelengths
For each member of ColumnSelection, the primary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushSecondary ExcitationWavelengths
For each member of ColumnSelection, the secondary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushTertiaryExcitation Wavelengths
For each member of ColumnSelection, the tertiary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushQuaternary ExcitationWavelengths
For each member of ColumnSelection, the quaternary monochromator/filter wavelength used to filter the lamp light before it is passed into the fluorescence flow cell to excite fluorescent compounds during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushEmission Wavelengths
For each member of ColumnSelection, the primary monochromator/filter wavelength used to filter emitted light from the fluorescence flow cell during column flush before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushSecondaryEmission Wavelengths
For each member of ColumnSelection, the secondary monochromator/filter wavelength used to filter emitted light from the fluorescence flow cell during column flush before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushTertiaryEmission Wavelengths
For each member of ColumnSelection, the tertiary monochromator/filter wavelength used to filter emitted light from the fluorescence flow cell during column flush before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushQuaternaryEmission Wavelengths
For each member of ColumnSelection, the quaternary monochromator/filter wavelength used to filter emitted light from the fluorescence flow cell during column flush before it is measured in the Fluorescence detector.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushEmissionCutOff Filters
For each member of ColumnSelection, the cut-off wavelength(s) to pre-select the emitted light from the flow downstream of the column and allow only the light with wavelength above the desired value to pass before the light enters emission monochromator for final wavelength selection during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushFluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnFlushExcitationWavelengths/ColumnFlushEmissionWavelengths channel during column flush on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnFlushSecondary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnFlushSecondaryExcitationWavelengths/ColumnFlushSecondaryEmissionWavelengths channel during column flush on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnFlushTertiary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnFlushTertiaryExcitationWavelengths/ColumnFlushTertiaryEmissionWavelengths channel during column flush on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnFlushQuaternary FluorescenceGains
For each member of ColumnSelection, the signal amplification of ColumnFlushQuaternaryExcitationWavelengths/ColumnFlushQuaternaryEmissionWavelengths channel during column flush on the Fluorescence detector.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
ColumnFlushFluorescence SamplingRates
For each member of ColumnSelection, the frequency of fluorescence measurement during column flush.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterEqualP[0 ------]]
Second
ColumnFlushMultiChannel FluorescenceSamplingRates
For each member of ColumnSelection, the frequency of fluorescence measurement during column flush when multiple fluorescence measurement channels are selected.
Format: Multiple
Class: Expression
Programmatic Pattern: HPLCFluorescenceSamplingRateP
ColumnFlushFluorescenceFlow CellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the fluorescence flow cell of the fluorescence detector during column flush.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnFlushLightScattering LaserPowers
For each member of ColumnSelection, the laser power used in the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) detector during column flush.
Format: Multiple
Class: Real
Unit: 1 percent
Programmatic Pattern: RangeP[10 Percent, 100 Percent]
ColumnFlushLightScattering FlowCellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the Multi-Angle static Light Scattering (MALS) and Dynamic Light Scattering (DLS) flow cell during column flush.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnFlushRefractiveIndex Methods
For each member of ColumnSelection, the type of refractive index measurement for the refractive index (RI) detector during column flush.
Format: Multiple
Class: Expression
Programmatic Pattern: RefractiveIndex | DifferentialRefractiveIndex
ColumnFlushRefractiveIndex FlowCellTemperatures
For each member of ColumnSelection, the nominal temperature setting inside the refractive index flow cell of the refractive index (RI) detector during column flush.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ColumnFlushCircularDichroism Wavelengths
For each member of ColumnSelection, the wavelength of the light used for circular dichroism measurement during column flush.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
ColumnFlushCircularDichroism SamplingRates
For each member of ColumnSelection, the frequency of circular dichroism measurement during column flush.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
ColumnFlushNebulizerGases
For each member of ColumnSelection, indicates if sheath gas is turned on for the Evaporative Light Scattering Detector (ELSD) during the column flush.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnFlushNebulizerGas Pressures
For each member of ColumnSelection, if NebulizerGas is True, the applied pressure to the sheath gas during the column flush.
Format: Multiple
Class: Real
Unit: 1 pound‐force per inch squared
Programmatic Pattern: RangeP[20 PSI, 60 PSI, 1 PSI]
ColumnFlushNebulizerGas Heatings
For each member of ColumnSelection, if NebulizerGas is True, indicates if sheath gas is heated or not for the Evaporative Light Scattering Detector (ELSD) during the column flush.
Format: Multiple
Class: Boolean
Programmatic Pattern: BooleanP
ColumnFlushNebulizerHeating Powers
For each member of ColumnSelection, the relative magnitude of the heating element used to heat the sheath gas during the column flush.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0 Percent, 100 Percent]
ColumnFlushDriftTube Temperatures
For each member of ColumnSelection, the temperature of the chamber through which the sprayed analyte travels during the column flush.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
ColumnFlushELSDGains
For each member of ColumnSelection, the signal amplification of the Evaporative Light Scattering Detector (ELSD) measurement during the column flush.
Format: Multiple
Class: Real
Programmatic Pattern: RangeP[0.01, 1000., 0.01]
ColumnFlushELSDSamplingRates
For each member of ColumnSelection, the frequency of measurement for the Evaporative Light Scattering Detector (ELSD) during the column flush.
Format: Multiple
Class: Real
Unit: 1 reciprocal second
Programmatic Pattern: 1
Hold[GreaterP[0 ------]]
Second
Last modified on Wed 9 Nov 2022 23:47:36