ECL`

AnalyzeBindingQuantitation

AnalyzeBindingQuantitation[QuantitationData]⟹Object

determines the concentration of the analyte measured in QuantitationData.

AnalyzeBindingQuantitation[DataObject]⟹Object

determines the concentration of the analyte measured by creating a standard curve by fitting all data with the specified model.

AnalyzeBindingQuantitation[ProtocolObject]⟹Object

determines the concentration of the analyte measured by creating a standard curve by fitting all data with the specified model.

Details

Analyte concentrations are obtained using a standard curve generated by fitting the observed association rate or equilibrium response of set of standard solutions with known concentrations of the target analyte.
Of the FittedParameter options, InitialRate will generally be the safest choice for assay where the analyte detection is performed in a single step. If a secondary detection solution is employed, AverageEquilibriumResponse may give more accurate results. In cases of slowly associating analytes that do not reach equilibrium, try using the FullRate, which fits the specified portion of the data using a 1:1 binding model.
Multiple data objects can be combined in a single analysis only if they use the same standard data.

Input

Output

Data Processing Options

Method Options

Examples

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Basic Examples (4)

Given an Object[Data,BioLayerInterferometry] with a standard, AnalyzeBindingQuantitation returns an Analysis Object:

Given an Object[Data,BioLayerInterferometry] with a standard, calculate the concentration of the input sample:

Given an Object[Protocol,BioLayerInterferometry], AnalyzeBindingQuantitation returns an Analysis Object:

Given multiple Object[Data,BioLayerInterferometry] with standards, AnalyzeBindingQuantitation returns an Analysis Object for each input:

Additional Examples (1)

AnalyzeBindingQuantitation populates the QuantitationAnalysis field with a data object containing the relevant fields:

Options (17)

Baselines (3)

Automatically populate the baseline if the data object contains QuantitationAnalyteBaselines:

Specify a constant baseline for each input data object:

Specify a single constant baseline to apply to all data objects:

FilterType (1)

Specify the type of filter to use to filter data for output plots:

FilterWidth (1)

Specify the radius of data points to treat with the filter type:

FinalFitDomain (2)

Automatically resolve the final domain based on the FittedParameter:

Specify the final domain to fit for all samples and standards:

FittedParameter (1)

Specify the parameter that should be fit to generate the standard curve:

InitialFitDomain (2)

Automatically resolve the final domain based on the FittedParameter:

Specify the initial domain to fit for all samples and standards:

StandardBaselines (2)

Automatically resolve the baselines to fit for the standard data based on the presence of the standard data:

Specify the baselines for the standard data:

StandardConcentrations (2)

Automatically resolve the concentrations of the dilutions used in StandardData when using data object input:

Specify the concentrations of the dilutions used in StandardData:

StandardCurveFit (1)

Specify a function to generate a standard curve:

StandardData (2)

Automatically populate the standard curve data if the data object contains QuantitationStandardDetection:

Specify data objects with QuantitationStandardDetection which is appropriate for the sample:

Messages (8)

Given an Object[Data,BioLayerInterferometry] without a standard, AnalyzeBindingQuantitation returns $Failed:

Baselines (2)

If an ambiguous number of baselines are given, an error is thrown:

When a data baseline is provided that is too short to span the data, an error is thrown:

Domains (1)

When both Final and InitialFitDomain are specified, an error is shown:

FinalFitDomain (1)

When the InitialFitDomain is inconsistent with the Fitted Parameter, a warning is displayed:

Fit (2)

When no standard data can be found an error is thrown:

When parameters are specified such that the data cannot be fit, a message will inform the user that the fitting has failed:

InitialFitDomain (1)