ExperimentIonChromatography
ExperimentIonChromatography[Samples]⟹Protocol
generates a Protocol for running an Ion Chromatography experiment to separate charged species from the provided Samples.
Ion Chromatography is a separation technique that resolves electrically-charged species. Once loaded into the system, samples are carried by the liquid mobile phase while migrating through the stationary phase column, within which charged resins are packed. The electrostatic interactions between the resin and the samples determine the time each species takes to travel through the column. Simultaneously, chemical eluent is introduced to the mobile phase to facilitate release of analytes from the column resin. Eluent can be mechanically pumped into the system or generated in the flow path via electrolysis. Following separation, the mobile phase enters a suppression module where the eluent with high conductivity undergoes chemical reactions to form low-conducting water, a process that effectively reduces the background signal from the eluent. As a result, the concentrations of each analyte is quantified with high sensitivity using conductivity measurement. Collectively, samples are separated into distinct molecular components based on their charges. Beyond this technique, a number of other methods rely on electrical charges of the analytes for separation, including ion-exchange chromatography in traditional HPLC as well as capillary electrophoresis. Owing to the suppressed conductivity detection, ion chromatography has a much higher sensitivity compared to both techniques, making it an ideal approach for analyzing trace metal ions in solution. In contrast, HPLC is better suited for preparative scale analysis with flexible detection methods; whereas, capillary electrophoresis offers efficient separation with low sample volume.
Experimental Principles
Figure 1.1: Procedural overview of an IonChromatography experiment. Step 1: Rinse the pumps, valves, and buffer lines within the instrument with cleaning solutions. Step 2: The stationary Column is installed and equilibrated to measurement conditions. Step 3: Samples are then injected into the system. Step 4: While traveling down the Column, the analytes are selectively retained on the resin based on their charges. Step 5: The chemical properties of the separated analytes are measured and plotted as function over retention time. Step 6: Upon completion of measurement, Column is rinsed with high concentration buffer solutions before storage. Step 7: Finally, the system is thoroughly flushed with cleaning solutions.
Instrumentation
Dionex ICS 6000
Figure 2.1.1: Schematic for Dionex ICS 6000 System. The instrument consists of 2 independent channels with different analytical capabilities. For anion channel, eluent is generated electrolytically from a cartridge and pumped through the system with an isocratic pump. For cation channel, up to 4 pre-made buffers are mixed at a set proportion and subsequently pumped through the system with a gradient pump. The buffers flow through a 6-port valve, a separation column, a suppressor, and a conductivity detector. After detection, the mixture is recycled as regenerant flow for the suppressor before exiting the system into the waste. Following a similar flow path, samples first are injected into the sample loop in the 6-port valve via the Autosampler. The rotation of the valve allows samples in the sample loop to migrate down the separation column where the analytes are separated based on their relative affinity to the column resin. The now separated analytes will enter a suppressor module to remove background signal contributed by buffers with high ionic strength before traveling to the conductivity detector.
Figure 2.1.2: Principle of eluent generation. A pair of electrodes is positioned on each side of an ion exchange membrane. When a current is applied to the electrodes, water molecules are electrolysed to generate hydroxide at the cathode and hydronium at the anode. The ion exchange membrane selectively allows potassium ions from the electrolyte reservoir to migrate across the membrane to form the eluent, Potassium Hydroxide (KOH). The eluent concentration is varied by changing the applied current to the electrodes.
Figure 2.1.3: Principle of suppressor. The role of a suppressor in Ion Chromatography is to remove the eluent and sample counter-ions and replace them with regenerant ions that have low level of conductivity. The presence of a suppressor in the flow path will significantly reduce detection background and therefore increase detection sensitivity. Specificatlly, in the Anion Suppressor, anode and cathod are separated by two cation exchange membranes. The water regenerant undergoes electrolysis in anode and cathod chambers. The cation exchange membrance then selectively allows the hydronium ions to move from the anode chamber to the eluent chamber, where these ions can neutralize the hydroxide eluent. At the same time, the potassium ions in the eluent are driven by the applied electric potential towards the cathode and combine with the hydroxide ions to form potassium hydroxide waste. Following a similar principle, the Cation Suppressor electrolyses water regenerant on the surface of the electrodes. The hydroxide ions then migrate through the anion exchange membrane from the cathod chamber to the eluent chamber to neutralize the acidic eluent. At the same time, the MSA ions travels to the anode chamber and combine with the hydronium ions to form methane sulfonic acid waste.
Dionex ICS 6000 with Electrochemical and UVVis Detector
Figure 2.2.1: Schematic for High Pressure Ion Chromatography instrument. Up to 4 pre-made buffer solutions are mixed at a set proportion and subsequently pumped through the system with a gradient pump. The buffers flow through a 6-port valve, a separation column, a variable wavelength detector, and an electrochemical detector. Following a similar flow path, samples are first injected into the sample loop in the 6-port valve via the Autosampler. The rotation of the valve allows samples to migrate down the separation column. The electrostatic interactions between the resin and the sample drives the separation of analytes into their molecular constituents. The now separated analytes will enter the detection module where they can elicit quantifiable signals as they pass through the flow cell.
Figure 2.2.2: Schematic for electrochemical detection. The electrochemical detector has 3 electrodes: the working electrode (WE), the counter electrode (CE), and the reference electrode (RE). A potential difference is applied between WE and CE, while the potential of RE stays constant throughout the measurement. As the analytes travel through the flow cell, diffusion will carry them to the surface of WE or CE where they will undergo redox reactions. The electrons liberated in these reactions will move directionally owing to the potential difference between the electrodes, generating quantifiable current signal that is proportional to the concentration of the analyte.
Experiment Options
General
AnalysisChannel
The flow path into which the sample is injected. CationChannel and AnionChannel employ suppressed conductivity detection for common cations and anions respectively whereas ElectrochemicalChannel employs electrochemical and/or UV detection for polysaccharides and beyond.
Default Calculation: Automatically set from the Composition and/or Analytes of the Samples. If the sample contains any oligomer, this option will automatically set to ElectrochemicalChannel. If the sample consists of two or more analytes in the common cation list (Lithium, Sodium, Ammonium, Potassium, Magnesium, and Calcium), this option will automatically set to CationChannel. If the sample consists of two or more analytes in the common anion list (Fluoride, Chloride, Bromide, Nitrate, Sulfate, and Phosphate), this option will automatically set to AnionChannel. If a sample meets both cation and anion criteria or if neither Composition nor Analytes is specified for the sample, this option defaults to AnionChannel.
Instrument
Pattern Description: An object of type or subtype Model[Instrument, IonChromatography] or Object[Instrument, IonChromatography]
Programmatic Pattern: ObjectP[{Model[Instrument, IonChromatography], Object[Instrument, IonChromatography]}] | Automatic
AnionSamples
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
CationSamples
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AnionColumn
A device with positively-charged resin through which the buffer and AnionSamples flow. It adsorbs and separates negatively-charged molecules within the sample based on the properties of the buffers, samples, and column temperature.
Default Calculation: Automatically set to a column model in stock with positively-charged stationary phase.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
CationColumn
A device with negatively-charged resin through which the buffer and CationSamples flow. It adsorbs and separates position-charged molecules within the sample based on the properties of the buffers, samples, and column temperature.
Default Calculation: Automatically set to a column model in stock with negatively-charged stationary phase.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
AnionGuardColumn
A protective device placed in the flow path before the AnionColumn in order to adsorb fouling contaminants and, thus, preserve the AnionColumn lifetime.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
CationGuardColumn
A protective device placed in the flow path before the CationColumn in order to adsorb fouling contaminants and, thus, preserve the CationColumn lifetime.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
AnionColumnTemperature
Default Calculation: Automatically set from the temperature within the AnionGradient option; otherwise, Ambient temperature is used.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
CationColumnTemperature
Default Calculation: Automatically set from the temperature within the CationGradient option; otherwise, Ambient temperature is used.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
AnionInjectionVolume
The physical quantity of AnionSamples loaded into the anion channel flow path for measurement. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Default Calculation: Automatically set from Instrument and takes into account Instrument dead volume and AnionSamples' volume.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
CationInjectionVolume
The physical quantity of CationSamples loaded into the cation channel flow path for measurement. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Default Calculation: Automatically set from Instrument and takes into account Instrument dead volume and CationSamples' volume.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
Detector
The type measurement to employ. For ElectrochemicalChannel, we currently offer UVVis (measures the absorbance of a single wavelength of light) and Electrochemical (measures the current generated in the oxidation and reduction of the analyte).
Default Calculation: Automatically set to both Electrochemical and UVVis detectors if ElectrochemicalChannel is used.
Pattern Description: List of one or more {SuppressedConductivity, UVVis, ElectrochemicalDetector} entries or {SuppressedConductivity, UVVis, ElectrochemicalDetector} or Null.
Programmatic Pattern: ((IonChromatographyDetectorTypeP | {IonChromatographyDetectorTypeP..}) | Automatic) | Null
Column
A device with electrically-charged resin through which the buffer and input samples flow. It adsorbs and separates molecules within the sample based on the properties of the buffers, samples, and column temperature.
Default Calculation: Automatically set to a column model in stock for carbohydrate analysis unless otherwise specified.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
GuardColumn
A protective device placed in the flow path before the Column in order to adsorb fouling contaminants and, thus, preserve the Column lifetime.
Pattern Description: An object of type or subtype Model[Item, Column] or Object[Item, Column] or Null.
ColumnTemperature
The temperature the Column, which is used in the electrochemical channel flow path, is held to throughout the separation and measurement.
Default Calculation: Automatically set from the temperature within the Gradient option; otherwise, Ambient temperature is used.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
InjectionVolume
The physical quantity of sample loaded into the electrochemical channel flow path for measurement. We recommend that injection volumes either be equal to the sample loop volume (25 uL) or less than or equal to one half of the sample loop volume (12.5 uL). Injection volumes between 12.5 and 25 uL may have lower accuracy and precision.
Default Calculation: Automatically set from Instrument and takes into account Instrument dead volume and the volume of the samples to be analyzed in the electrochemical channel.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 25 microliters or Null.
BufferA
The solvent pumped through channel A of the flow path. For instrument having more than one analysis channels, BufferA is only pumped into the cation channel. The anion channel features an eluent generator in the flow path capable of generating up to 100 Millimolar of Potassium Hydroxide through hydrolysis of deionized water.
Default Calculation: Automatically set from the objects specified by the Gradient option for the instrument with ElectrochemicalChannel or CationGradient option for the instrument with AnionChannel and CationChannel. If Gradient or CationGradient option is not specified, this option defaults to 500 mM Sodium Hydroxide for samples injected into ElectrochemicalChannel and 50 mM Methanesulfonic Acid for those injected into CationChannel.
Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
BufferAStorageCondition
The conditions under which BufferA used by this experiment should be stored after the protocol is completed. If this option is set to Null when BufferA is specified, BufferA will be stored according to the Models' DefaultStorageCondition.
Default Calculation: Automatically set based on the final volume of BufferA. If the final volume is more than 500 Milliliter, BufferA will be stored according to the Models' DefaultStorageCondition. Otherwise, BufferA will be disposed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BufferB
The solvent pumped through channel B of the flow path. For instrument having more than one analysis channels, BufferB is only pumped into the cation channel. The anion channel features an eluent generator in the flow path capable of generating up to 100 Millimolar of Potassium Hydroxide through hydrolysis of deionized water.
Default Calculation: Automatically set from the objects specified by the Gradient option for the instrument with ElectrochemicalChannel or CationGradient option for the instrument with AnionChannel and CationChannel. If Gradient or CationGradient option is not specified, this option defaults to 500 mM Sodium Acetate for samples injected into ElectrochemicalChannel and Milli-Q water for those injected into CationChannel.
Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
BufferBStorageCondition
The conditions under which BufferB used by this experiment should be stored after the protocol is completed. If this option is set to Null when BufferB is specified, BufferB will be stored according to the Models' DefaultStorageCondition.
Default Calculation: Automatically set based on the final volume of BufferB. If the final volume is more than 500 Milliliter, BufferB will be stored according to the Models' DefaultStorageCondition. Otherwise, BufferB will be disposed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BufferC
The solvent pumped through channel C of the flow path. For instrument having more than one analysis channels, BufferC is only pumped into the cation channel. The anion channel features an eluent generator in the flow path capable of generating up to 100 Millimolar of Potassium Hydroxide through hydrolysis of deionized water.
Default Calculation: Automatically set from the objects specified by the Gradient option for the instrument with ElectrochemicalChannel or CationGradient option for the instrument with AnionChannel and CationChannel. If Gradient or CationGradient option is not specified, this option defaults to Milli-Q water for samples injected into ElectrochemicalChannel or CationChannel.
Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
BufferCStorageCondition
The conditions under which BufferC used by this experiment should be stored after the protocol is completed. If this option is set to Null when BufferC is specified, BufferC will be stored according to the Models' DefaultStorageCondition.
Default Calculation: Automatically set based on the final volume of BufferC. If the final volume is more than 500 Milliliter, BufferC will be stored according to the Models' DefaultStorageCondition. Otherwise, BufferC will be disposed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BufferD
The solvent pumped through channel D of the flow path. For instrument having more than one analysis channels, BufferD is only pumped into the cation channel. The anion channel features an eluent generator in the flow path capable of generating up to 100 Millimolar of Potassium Hydroxide through hydrolysis of deionized water.
Default Calculation: Automatically set from the objects specified by the Gradient option for single-channel instrument and CationGradient option for dual-channel instrument.
Pattern Description: An object of type or subtype Object[Sample] or Model[Sample] or a prepared sample or Null.
BufferDStorageCondition
The conditions under which BufferD used by this experiment should be stored after the protocol is completed. If this option is set to Null when BufferC is specified, BufferD will be stored according to the Models' DefaultStorageCondition.
Default Calculation: Automatically set based on the final volume of BufferD. If the final volume is more than 500 Milliliter, BufferD will be stored according to the Models' DefaultStorageCondition. Otherwise, BufferD will be disposed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
NumberOfReplicates
The number of times to repeat measurements on each provided sample(s). If Aliquot->True, this also indicates the number of times each provided sample will be aliquoted.
EluentGradient
The concentration of the eluent, potassium hydroxide, that is automatically generated within the flow in anion channel, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for EluentGradient->{{0 Minute, 0 Millimolar},{30 Minute, 100 Millimolar}}, the eluent concentration in the flow will rise such that at 15 minutes, the concentration should be 50 Millimolar. Since eluent generator is only integrated in anion channel, this option is not applicable to cation samples. Gradient for cation samples can be set in CationGradientA, CationGradientB, CationGradientC, CationGradientD, and CationGradient options.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or list of one or more {Time, Eluent Concentration} entries or Null.
Programmatic Pattern: ((RangeP[0*Millimolar, 100*Millimolar] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar]}..}) | Automatic) | Null
StandardEluentGradient
The concentration of the eluent, potassium hydroxide, that is automatically generated within the flow path for AnionStandard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for StandardEluentGradient->{{0 Minute, 0 Millimolar},{30 Minute, 100 Millimolar}}, the eluent concentration in the flow will rise such that at 15 minutes, the eluent concentration should be 50 Millimolar. Since eluent generator is only integrated in anion channel, this option is not applicable to cation standards. Gradient for cation standards can be set in CationStandardGradientA, CationStandardGradientB, CationStandardGradientC, CationStandardGradientD, and CationStandardGradient options.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or list of one or more {Time, Eluent Concentration} entries or Null.
Programmatic Pattern: ((RangeP[0*Millimolar, 100*Millimolar] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar]}..}) | Automatic) | Null
Gradient
AnionFlowRate
The speed of the fluid through the pump for anion channel. This speed is linearly interpolated such that consecutive entries of {Time, AnionFlowRate} will define the intervening fluid speed. For example, {{0 Minute, 0.3 Milliliter/Minute},{30 Minute, 0.5 Milliliter/Minute}} means flow rate of 0.4 Milliliter/Minute at 15 minutes into the run.
Default Calculation: Automatically set from AnionColumn or taken from the method given in the AnionGradient option. If neither option is specified, the smaller flow rate between the minumum flow rate of the resolved AnionColumn and that of the resolved Instrument will be used.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionGradientStart
A shorthand option to specify the starting eluent concentration in the fluid flow for anion channel.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionGradientEnd
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionEquilibrationTime
A shorthand option to specify the duration of equilibration for anion channel at the starting eluent concentration at the onset of the gradient.
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionFlushTime
A shorthand option to specify the duration of buffer flush for anion channel at the end of the gradient.
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionGradient
The concentration of the eluent, potassium hydroxide, over time in the fluid flow for anion channel. Specific parameters of an object can be overridden by specific options for EluentGradient.
Default Calculation: Automatically set to match all specified options related to AnionGradient (e.g. EluentGradient, AnionFlowRate, AnionGradientStart, AnionGradientEnd, AnionGradientDuration, AnionEquilibrateTime, AnionFlushTime). If none of the options is specified, the following default gradient will be used: {{0 Minute, 10 Millimolar, DefaultAnionFlowRate},{0.1 Minute, 10 Millimolar, DefaultAnionFlowRate}, {12 Minute, 22 Millimolar, DefaultAnionFlowRate}, {20 Minute, 50 Millimolar, DefaultAnionFlowRate}, {20.5 Minute], 50 Millimolar, DefaultAnionFlowRate}, {20.6 Minute, 10 Millimolar, DefaultFlowRate}, {27 Minute, 10 Millimolar, DefaultAnionFlowRate}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Eluent Concentration, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationGradientA
The composition of Buffer A within the flow in cation channel, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferA is only pumped into cation channel, this option is not applicable to anion samples. Gradient for anion samples can be set in EluentGradient and AnionGradient options.
Default Calculation: Automatically set from CationGradient option or implicitly determined from CationGradientB, CationGradientC, and CationGradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationGradientB
The composition of Buffer B within the flow in cation channel, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferB is only pumped into cation channel, this option is not applicable to anion samples. Gradient for anion samples can be set in EluentGradient and AnionGradient options.
Default Calculation: Automatically set from CationGradient option or implicitly determined from CationGradientA, CationGradientC, and CationGradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationGradientC
The composition of Buffer C within the flow in cation channel, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferC is only pumped into cation channel, this option is not applicable to anion samples. Gradient for anion samples can be set in EluentGradient and AnionGradient options.
Default Calculation: Automatically set from CationGradient option or implicitly determined from CationGradientA, CationGradientB, and CationGradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationGradientD
The composition of Buffer D within the flow in cation channel, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferD is only pumped into cation channel, this option is not applicable to anion samples. Gradient for anion samples can be set in EluentGradient and AnionGradient options.
Default Calculation: Automatically set from CationGradient option or implicitly determined from CationGradientA, CationGradientB, and CationGradientC options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationFlowRate
The speed of the fluid through the pump for cation channel. This speed is linearly interpolated such that consecutive entries of {Time, CationFlowRate} will define the intervening fluid speed. For example, {{0 Minute, 0.3 Milliliter/Minute},{30 Minute, 0.5 Milliliter/Minute}} means flow rate of 0.4 Milliliter/Minute at 15 minutes into the run.
Default Calculation: Automatically set from CationColumn or taken from the method given in the CationGradient option. If neither option is specified, the smaller flow rate between the minumum flow rate of the resolved CationColumn and that of the resolved Instrument will be used.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationGradientStart
A shorthand option to specify the starting BufferB composition in the fluid flow for cation channel.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationGradientEnd
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationEquilibrationTime
A shorthand option to specify the duration of equilibration for cation channel at the starting buffer composition at the onset of the gradient.
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationFlushTime
A shorthand option to specify the duration of buffer flush for cation channel at the end of the gradient.
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationGradient
The buffer composition over time in the fluid flow for cation channel. Specific parameters of an object can be overridden by specific options for CationGradientA, CationGradientB, CationGradientC, and CationGradientD.
Default Calculation: Automatically set to match all specified options releated to CationGradient (e.g. CationGradientA, CationGradientB, CationGradientC, CationGradientD, CationFlowRate, CationGradientStart, CationGradientEnd, CationGradientDuration, CationEquilibrateTime, and CationFlushTime). If none of the options is specified, the following default gradient will be used: {{0 Minute, 60 Percent, 40 Percent, 0 Percent, 0 Percent, DefaultCationFlowRate}, {23 Minute, 60 Percent, 40 Percent, 0 Percent, 0 Percent, DefaultCationFlowRate}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
GradientA
The composition of Buffer A within the flow the instrument, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for GradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from Gradient option or implicitly determined from GradientB, GradientC, and GradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
GradientB
The composition of Buffer B within the flow the instrument, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for GradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from Gradient option or implicitly determined from GradientA, GradientC, and GradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
GradientC
The composition of Buffer C within the flow of the instrument, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for GradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from Gradient option or implicitly determined from GradientA, GradientB, and GradientD options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
GradientD
The composition of Buffer D within the flow of the instrument, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for GradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from Gradient option or implicitly determined from GradientA, GradientB, and GradientC options such that the composition of Buffer A, B, C and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
FlowRate
The speed of the fluid through the pump. This speed is linearly interpolated such that consecutive entries of {Time, Flow Rate} will define the intervening fluid speed. For example, {{0 Minute, 0.3 Milliliter/Minute},{30 Minute, 0.5 Milliliter/Minute}} means flow rate of 0.4 Milliliter/Minute at 15 minutes into the run.
Default Calculation: Automatically set from the method given in the Gradient option. If neither option is specified, the smaller flow rate between the minumum flow rate of the resolved Column and that of the resolved Instrument will be used.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
GradientStart
A shorthand option to specify the starting BufferB concentration in the fluid flow of the instrument.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
GradientEnd
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
GradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
EquilibrationTime
A shorthand option to specify the duration of equilibration at the starting buffer concentration at the onset of the gradient.
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
FlushTime
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
Gradient
The concentration of buffer over time in the fluid flow of the instrument. Specific parameters of an object can be overridden by specific options for GradientA, GradientB, GradientC, and GradientD.
Default Calculation: Automatically set to match all specified options related to Gradient (e.g. GradientA, GradientB, GradientC, GradientD, FlowRate, GradientStart, GradientEnd, GradientDuration, EquilibrateTime, FlushTime).
Pattern Description: An object of type or subtype Object[Method, Gradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Gradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
Detection
AnionSuppressorCurrent
The electrical current supplied to the AnionSuppressor module where high-conducting buffer is replaced by low-conducting water through electrolysis and chemical reactions.
Default Calculation: This option will be calculated by multiplying flow rate, maximum eluent concentration and suppressor specific factor (2.47 for anion suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 100 milliamperes or Null.
AnionDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if AnionChannel is in use and Null if not.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
CationSuppressorCurrent
The electrical current supplied to the CationSuppressor module where high-conducting Buffer is replaced by low-conducting water through electrolysis and chemical reactions.
Default Calculation: This option will be calculated by multiplying flow rate, maximum buffer concentration and suppressor specific factor (2.94 for cation suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 100 milliamperes or Null.
CationDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if CationChannel is in use and Null if not.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
AbsorbanceWavelength
The physical properties of light passed through the flow for the UVVis Detector. Up to 4 separate wavelengths may be specified.
Default Calculation: Automatically set to 280 Nanometer if the UVVis detector is used in the protocol.
Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or list of one or more greater than or equal to 190 nanometers and less than or equal to 900 nanometers entries or Null.
Programmatic Pattern: ((RangeP[190*Nanometer, 900*Nanometer] | {RangeP[190*Nanometer, 900*Nanometer]..}) | Automatic) | Null
AbsorbanceSamplingRate
Indicates the frequency of absorbance measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set based on the number of wavelengths specified. Default AbsorbanceSamplingRate is 10/Second for measurement at a single wavelength and 1/Second for measurements at multiple wavelengths.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
ElectrochemicalDetectionMode
Specifies the mode of operation for the electrochemical detector, including DC Amperometric Detection, Pulsed Amperometric Detection, and Integrated Pulsed Amperometric Detection. In DC Amperometric Detection, a constant voltage is applied. In contrast, Pulsed Amperometric Detections first apply a working potential followed by higher or lower potentials that are used for cleaning the electrode. Further, Integrated Amperometric Detection integrates current over a single potential whereas Integrated Pulsed Amperometric Detection integrates current over two or more potentials.
Default Calculation: Automatically set based on the VoltageProfile or the WaveformProfile specified. If neither option is specified, this option defaults to Pulsed Amperometry.
Pattern Description: {DCAmperometricDetection, PulsedAmperometricDetection, IntegratedPulsedAmperometricDetection} or Null.
pHCalibration
Indicates whether the reference electrode (combination pH-Ag/AgCl electrode) needs to be calibrated against standard pH solutions before samples are injected.
Default Calculation: Automatically set to False if electrochemical detector is used. Otherwise resolves to Null.
NeutralpHCalibrationBuffer
Default Calculation: Automatically set to the reference solution with a pH of 7 if pHCalibration is True.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
SecondarypHCalibrationBuffer
The additional solution, either acidic or basic, used to create the second point on the pH calibration curve.
Default Calculation: Automatically set to the reference solution with a pH value of 10 if pHCalibration is True.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
SecondarypHCalibrationBufferTarget
Default Calculation: Automatically set to pH of the specified SecondarypHCalibrationBuffer if pHCalibration is True.
WorkingElectrode
The electrode where the analytes undergo reduction or oxidation recations due to the potential difference applied.
Default Calculation: Automatically set to Model[Item, Electrode, "Disposable Gold on PTFE Electrode"] if electrochemical detection is employed.
Pattern Description: An object of type or subtype Object[Item, Electrode] or Model[Item, Electrode] or Null.
Programmatic Pattern: (ObjectP[{Object[Item, Electrode], Model[Item, Electrode]}] | Automatic) | Null
WorkingElectrodeStorageCondition
The conditions under which the WorkingElectrode used by this experiment should be stored after the protocol is completed. If this option is set to Null when WorkingElectrode is specified, WorkingElectrode will be stored according to the Models' DefaultStorageCondition.
Default Calculation: Automatically set to Models' DefaultStorageCondition if WorkingElectrode is specified.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
ReferenceElectrodeMode
The operating mode (either pH or AgCl) of the pH-Ag/AgCl reference electrode. The pH-Ag/AgCl reference electrode offers two distinct modes of operation: it can function as a "pH" reference for monitoring buffer pH or serve as an "AgCl" reference with a constant potential in electrochemical cells.
Default Calculation: Automatically set from Waveform method object. If Waveform is not specified, this option defaults to AgCl.
VoltageProfile
Default Calculation: Automatically set to 0.1 Volt for DCAmperometry. If any other ElectrochemicalDetectionMode is used, this option will set to Null.
Pattern Description: Greater than or equal to -2.048 volts and less than or equal to 2.047 volts or list of one or more {Time, Voltage} entries or Null.
Programmatic Pattern: ((RangeP[-2.048*Volt, 2.047*Volt] | {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..}) | Automatic) | Null
WaveformProfile
A series of time-dependent voltage setting (waveform) that will be repeated over the duration of the analysis.
Default Calculation: Automatically set to repeat a single waveform defined for either Pulsed Amperometric Detection or Integrated Pulsed Amperometric Detection over the entire run.
Pattern Description: An object of type or subtype Object[Method, Waveform] or list of one or more {Time, Waveform} entries or list of one or more {Time, Waveform} entries or list of one or more {Waveform Time, Voltage, Interpolation, Integration} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Waveform]] | {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..} | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..}) | Automatic) | Null
ElectrochemicalSamplingRate
Indicates the frequency of amperometric measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set to 1/waveform duration if a single waveform is specified. If multiple waveforms are specified, this option is set to 1 over the longest waveform duration. If no waveform is specified, this option is set to 2/Second.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
DetectionTemperature
Default Calculation: This option will automatically be set to Ambient if electrochemical detector is used.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
Standard
Standard
A reference compound to inject to the instrument, often used for quantification or to check internal measurement consistency.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
StandardStorageCondition
The conditions under which any Standard used by this experiment should be stored after the protocol is completed. If this option is left as Automatic when Standard samples are specified, the Standard samples will be stored according to their Models' DefaultStorageCondition.
Default Calculation: Automatically set based on the final volume of Standard samples. If the final volume is more than 500 Microliter, Standard samples will be stored according to the Models' DefaultStorageCondition. Otherwise, Standard samples will be disposed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
StandardAnalysisChannel
The flow path into which the Standard is injected. CationChannel and AnionChannel employ suppressed conductivity detection for common cations and anions respectively whereas ElectrochemicalChannel employs electrochemical and/or UV detection for polysaccharides and beyond.
Default Calculation: Automatically set from the Composition and/or Analytes of the Standard. If the sample contains any oligomer, this option will automatically set to ElectrochemicalChannel. If the sample consists of two or more analytes in the common cation list (Lithium, Sodium, Ammonium, Potassium, Magnesium, and Calcium), this option will automatically set to CationChannel. If the sample consists of two or more analytes in the common anion list (Fluoride, Chloride, Bromide, Nitrate, Sulfate, and Phosphate), this option will automatically set to AnionChannel. If a sample meets both cation and anion criteria or if neither Composition nor Analytes is specified for the sample, this option defaults to AnionChannel.
AnionStandard
Default Calculation: Automatically compiled from Standard with StandardAnalysisChannel set to AnionChannel.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AnionStandardFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
CationStandard
Default Calculation: Automatically compiled from Standard with StandardAnalysisChannel set to CationChannel.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
CationStandardFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
AnionStandardInjectionVolume
The physical quantity of each AnionStandard to inject into the system. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
AnionStandardColumnTemperature
Default Calculation: Automatically set from AnionColumnTemperature or taken from the AnionStandardGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
CationStandardInjectionVolume
The physical quantity of each CationStandard to inject into the system. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
CationStandardColumnTemperature
Default Calculation: Automatically set from CationColumnTemperature or taken from the CationStandardGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
AnionStandardFlowRate
Default Calculation: Automatically set from AnionColumn, and FlowRate specified in the AnionInjectionTable or taken from the method given in the AnionStandardGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionStandardGradientStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for AnionStandard samples.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionStandardGradientEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for AnionStandard samples.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionStandardGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionStandardGradient
The concentration of the eluent, potassium hydroxide, over time in the fluid flow for AnionStandard samples. Specific parameters of an object can be overridden by specific options for StandardEluentGradient. Since eluent generator is only integrated in anion channel, this option is not applicable to cation standards. Gradient for cation standards can be set in CationStandardGradientA, CationStandardGradientB, CationStandardGradientC, CationStandardGradientD, and CationStandardGradient options.
Default Calculation: Automatically set to match all specified options related to AnionStandardGradient (e.g. StandardEluentGradient, AnionStandardFlowRate, AnionStandardGradientDuration). If none of the options is specified, the first value of AnionGradient will be used.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Eluent Concentration, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionStandardSuppressorCurrent
The electrical current supplied to the AnionSuppressor module for AnionStandard samples before conductivity measurement.
Default Calculation: This option will be calculated by multiplying flow rate, maximum eluent concentration and suppressor specific factor (2.47 for anion suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
AnionStandardDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
CationStandardGradientA
The composition of Buffer A within the flow for CationStandard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationStandardGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferA is only pumped into cation channel, this option is not applicable to anion standards. Gradient for anion standards can be set in StandardEluentGradient and AnionStandardGradient options.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from CationStandardGradientB, CationStandardGradientC, and CationStandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationStandardGradientB
The composition of Buffer B within the flow for CationStandard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationStandardGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferB is only pumped into cation channel, this option is not applicable to anion standards. Gradient for anion standards can be set in StandardEluentGradient and AnionStandardGradient options.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from CationStandardGradientA, CationStandardGradientC, and CationStandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationStandardGradientC
The composition of Buffer C within the flow for CationStandard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationStandardGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferC is only pumped into cation channel, this option is not applicable to anion standards. Gradient for anion standards can be set in StandardEluentGradient and AnionStandardGradient options.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from CationStandardGradientA, CationStandardGradientB, and CationStandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationStandardGradientD
The composition of Buffer D within the flow for CationStandard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationStandardGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferD is only pumped into cation channel, this option is not applicable to anion standards. Gradient for anion standards can be set in StandardEluentGradient and AnionStandardGradient options.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from CationStandardGradientA, CationStandardGradientB, and CationStandardGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationStandardFlowRate
Default Calculation: Automatically set from CationColumn, and FlowRate specified in the CationInjectionTable or taken from the method given in the CationStandardGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationStandardGradientStart
A shorthand option to specify the starting BufferB concentration in the fluid flow of the instrument for CationStandard samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationStandardGradientEnd
A shorthand option to specify the final BufferB concentration in the fluid flow of the instrument for CationStandard samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationStandardGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationStandardGradient
The buffer composition over time in the fluid flow for CationStandard samples. Specific parameters of an object can be overridden by specific options for CationStandardGradientA, CationStandardGradientB, CationStandardGradientC, and CationStandardGradientD.
Default Calculation: Automatically set to match all specified options related to CationStandardGradient (e.g. CationStandardGradientA, CationStandardGradientB, CationStandardGradientC, CationStandardGradientD, CationStandardFlowRate, and CationStandardGradientDuration). If none of the options is specified, the first value of CationGradient will be used.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationStandardSuppressorCurrent
The electrical current supplied to the CationSuppressor module for CationStandard samples before conductivity measurement.
Default Calculation: This option will be calculated by multiplying flow rate, maximum buffer concentration and suppressor specific factor (2.94 for cation suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
CationStandardDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
StandardFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
StandardInjectionVolume
The physical quantity of each Standard to inject into the system. We recommend that injection volumes either be equal to the sample loop volume (25 uL) or less than or equal to one half of the sample loop volume (12.5 uL). Injection volumes between 12.5 and 25 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 25 microliters or Null.
StandardColumnTemperature
Default Calculation: Automatically set from ColumnTemperature or taken from the StandardGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
StandardGradientA
The composition of Buffer A within the flow for Standard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for StandardGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from StandardGradient option or implicitly determined from StandardGradientB, StandardGradientC, and StandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
StandardGradientB
The composition of Buffer B within the flow for Standard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for StandardGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from StandardGradientA, StandardGradientC, and StandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
StandardGradientC
The composition of Buffer C within the flow for Standard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for StandardGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from CationStandardGradient option or implicitly determined from StandardGradientA, StandardGradientB, and StandardGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
StandardGradientD
The composition of Buffer D within the flow for Standard samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for StandardGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from StandardGradient option or implicitly determined from StandardGradientA, StandardGradientB, and StandardGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
StandardFlowRate
Default Calculation: Automatically set from Column, and FlowRate specified in the InjectionTable or taken from the method given in the StandardGradient option. If neither option is specified, the first value of FlowRate will be used.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
StandardGradientStart
A shorthand option to specify the starting BufferB concentration in the fluid flow of the instrument for Standard samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
StandardGradientEnd
A shorthand option to specify the final BufferB concentration in the fluid flow of the instrument for Standard samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
StandardGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
StandardGradient
The buffer composition over time in the fluid flow for Standard samples. Specific parameters of an object can be overridden by specific options for StandardGradientA, StandardGradientB, StandardGradientC, and StandardGradientD.
Default Calculation: Automatically set to match all specified options related to StandardGradient (e.g. StandardGradientA, StandardGradientB, StandardGradientC, StandardGradientD, StandardFlowRate, and StandardGradientDuration). If none of the options is specified, the first Gradient will be used.
Pattern Description: An object of type or subtype Object[Method, Gradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Gradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
StandardAbsorbanceWavelength
The physical properties of light passed through the flow for the UVVis Detector during Standard sample runs. Up to 4 separate wavelengths may be specified.
Default Calculation: Automatically set to 280 Nanometer if the UVVis detector is used in the protocol.
Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or list of one or more greater than or equal to 190 nanometers and less than or equal to 900 nanometers entries or Null.
Programmatic Pattern: ((RangeP[190*Nanometer, 900*Nanometer] | {RangeP[190*Nanometer, 900*Nanometer]..}) | Automatic) | Null
StandardAbsorbanceSamplingRate
Indicates the frequency of absorbance measurement for Standard samples. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set based on the number of wavelengths specified. Default StandardAbsorbanceSamplingRate is 10/Second for measurement at a single wavelength and 1/Second for measurements at multiple wavelengths.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
StandardElectrochemicalDetectionMode
Specifies the mode of operation for the electrochemical detector during Standard sample runs, including DC Amperometric Detection, Pulsed Amperometric Detection, and Integrated Pulsed Amperometric Detection. In DC Amperometric Detection, a constant voltage is applied. In contrast, Pulsed Amperometric Detections first apply a working potential followed by higher or lower potentials that are used for cleaning the electrode. Further, Integrated Amperometric Detection integrates current over a single potential whereas Integrated Pulsed Amperometric Detection integrates current over two or more potentials.
Default Calculation: Automatically set based on the StandardVoltageProfile or the StandardWaveformProfile specified. If neither option is specified, this option defaults to Pulsed Amperometry.
Pattern Description: {DCAmperometricDetection, PulsedAmperometricDetection, IntegratedPulsedAmperometricDetection} or Null.
StandardReferenceElectrodeMode
A combination pH-Ag/AgCl reference electrode that can be used to either monitor the buffer pH ("pH" reference) or to serve as a cell reference electrode with a constant potential ("AgCl" reference).
Default Calculation: Automatically set from Waveform method object. If Waveform is not specified, this option defaults to AgCl.
StandardVoltageProfile
Default Calculation: Automatically set to 0.1 Volt for DCAmperometricDetection. If any other StandardElectrochemicalDetectionMode is used, this option will set to Null.
Pattern Description: Greater than or equal to -2.048 volts and less than or equal to 2.047 volts or list of one or more {Time, Voltage} entries or Null.
Programmatic Pattern: ((RangeP[-2.048*Volt, 2.047*Volt] | {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..}) | Automatic) | Null
StandardWaveformProfile
A series of time-dependent voltage setting (waveform) that will be repeated over the duration of the Standard sample analysis.
Default Calculation: Automatically set to repeat a single waveform defined for either Pulsed Amperometric Detection or Integrated Pulsed Amperometric Detection over the entire run.
Pattern Description: An object of type or subtype Object[Method, Waveform] or list of one or more {Time, Waveform} entries or list of one or more {Time, Waveform} entries or list of one or more {Waveform Time, Voltage, Interpolation, Integration} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Waveform]] | {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..} | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{GreaterEqualP[0*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..}) | Automatic) | Null
StandardElectrochemicalSamplingRate
Indicates the frequency of amperometric measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set to 1/waveform duration if a single waveform is specified. If multiple waveforms are specified, this option is set to 1 over the longest waveform duration. If no waveform is specified, this option is set to 2/Second.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
StandardDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if electrochemical detector is used.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
Blanks
Blank
The compound(s) to inject typically as negative controls (e.g. to test effects stemming from injection, sample solvent, or buffer).
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BlankStorageCondition
The conditions under which any Blank sample used by this experiment should be stored after the protocol is completed. If this option is set to Null when Blank samples are specified, the Blank samples will be stored according to their Models' DefaultStorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
BlankAnalysisChannel
The flow path into which the Blank is injected. CationChannel and AnionChannel employ suppressed conductivity detection for common cations and anions respectively whereas ElectrochemicalChannel employs electrochemical and/or UV detection for polysaccharides and beyond.
Default Calculation: Automatically set from the Composition and/or Analytes of the Blank. If the sample contains any oligomer, this option will automatically set to ElectrochemicalChannel. If the sample consists of two or more analytes in the common cation list (Lithium, Sodium, Ammonium, Potassium, Magnesium, and Calcium), this option will automatically set to CationChannel. If the sample consists of two or more analytes in the common anion list (Fluoride, Chloride, Bromide, Nitrate, Sulfate, and Phosphate), this option will automatically set to AnionChannel. If a sample meets both cation and anion criteria or if neither Composition nor Analytes is specified for the sample, this option defaults to AnionChannel.
AnionBlank
Default Calculation: Automatically compiled from Blank with BlankAnalysisChannel set to AnionChannel.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AnionBlankFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
CationBlank
Default Calculation: Automatically compiled from Blank with BlankAnalysisChannel set to CationChannel.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
CationBlankFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
AnionBlankInjectionVolume
The physical quantity of each AnionBlank sample to inject. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
AnionBlankColumnTemperature
Default Calculation: Automatically set from AnionColumnTemperature or taken from the AnionBlankGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
CationBlankInjectionVolume
The physical quantity of each CationBlank sample to inject. We recommend that injection volumes either be equal to the sample loop volume (10 uL) or less than or equal to one half of the sample loop volume (5 uL). Injection volumes between 5 and 10 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 10 microliters or Null.
CationBlankColumnTemperature
Default Calculation: Automatically set from CationColumnTemperature or taken from the CationBlankGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
BlankEluentGradient
The concentration of the eluent that is automatically generated within the flow path for AnionBlank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankEluentGradient->{{0 Minute, 0 Millimolar},{30 Minute, 100 Millimolar}}, the eluent concentration in the flow will rise such that at 15 minutes, the concentration should be 50 Millimolar. Since eluent generator is only integrated in anion channel, this option is not applicable to cation blanks. Gradient for cation blanks can be set in CationBlankGradientA, CationBlankGradientB, CationBlankGradientC, CationBlankGradientD, and CationBlankGradient options.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or list of one or more {Time, Eluent Concentration} entries or Null.
Programmatic Pattern: ((RangeP[0*Millimolar, 100*Millimolar] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar]}..}) | Automatic) | Null
AnionBlankFlowRate
Default Calculation: Automatically set from AnionColumn and FlowRate specified in the AnionInjectionTable or taken from the method given in the AnionBlankGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionBlankGradientStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for AnionBlank samples.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionBlankGradientEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for AnionBlank samples.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionBlankGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionBlankGradient
The concentration of the eluent, potassium hydroxide, over time in the fluid flow for AnionBlank samples. Specific parameters of an object can be overridden by specific options for BlankEluentGradient. Since eluent generator is only integrated in anion channel, this option is not applicable to cation blanks. Gradient for cation blanks can be set in CationBlankGradientA, CationBlankGradientB, CationBlankGradientC, CationBlankGradientD, and CationBlankGradient options.
Default Calculation: Automatically set to match all specified options related to AnionBlankGradient (e.g. BlankEluentGradient, AnionBlankFlowRate, AnionBlankGradientDuration). If none of the options is specified, the first AnionGradient will be used.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Eluent Concentration, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionBlankSuppressorCurrent
The electrical current supplied to the AnionSuppressor module for AnionBlank samples before conductivity measurement.
Default Calculation: This option will be calculated by multiplying flow rate, maximum eluent concentration and suppressor specific factor (2.47 for anion suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
AnionBlankDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
CationBlankGradientA
The composition of Buffer A within the flow for CationBlank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationBlankGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferA is only pumped into cation channel, this option is not applicable to anion blanks. Gradient for anion blanks can be set in BlankEluentGradient and AnionBlankGradient options.
Default Calculation: Automatically set from CationBlankGradient option or implicitly determined from CationBlankGradientA, CationBlankGradientB, and CationBlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationBlankGradientB
The composition of Buffer B within the flow for CationBlank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationBlankGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferB is only pumped into cation channel, this option is not applicable to anion blanks. Gradient for anion blanks can be set in BlankEluentGradient and AnionBlankGradient options.
Default Calculation: Automatically set from CationBlankGradient option or implicitly determined from CationBlankGradientA, CationBlankGradientC, and CationBlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationBlankGradientC
The composition of Buffer C within the flow for CationBlank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationBlankGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferC is only pumped into cation channel, this option is not applicable to anion blanks. Gradient for anion blanks can be set in BlankEluentGradient and AnionBlankGradient options.
Default Calculation: Automatically set from CationBlankGradient option or implicitly determined from CationBlankGradientA,CationBlankGradientB, and CationBlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationBlankGradientD
The composition of Buffer D within the flow for CationBlank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferD is only pumped into cation channel, this option is not applicable to anion blanks. Gradient for anion blanks can be set in BlankEluentGradient and AnionBlankGradient options.
Default Calculation: Automatically set from CationBlankGradient option or implicitly determined from CationBlankGradientA, CationBlankGradientB, and CationBlankGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationBlankFlowRate
Default Calculation: Automatically set from CationColum and FlowRate specified in the CationInjectionTable or taken from the method given in the CationBlankGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationBlankGradientStart
A shorthand option to specify the starting BufferB concentration in the fluid flow of the instrument for CationBlank samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationBlankGradientEnd
A shorthand option to specify the final BufferB concentration in the fluid flow of the instrument for CationBlank samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationBlankGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationBlankGradient
The buffer composition over time in the fluid flow for CationBlank samples. Specific parameters of an object can be overridden by specific options for CationBlankGradientA, CationBlankGradientB, CationBlankGradientC, and CationBlankGradientD.
Default Calculation: Automatically set to match all specified options related to CationBlankGradient (e.g. CationBlankGradientA, CationBlankGradientB, CationBlankGradientC, CationBlankGradientD, CationBlankFlowRate, and CationBlankGradientDuration). If none of the options is specified, the first CationGradient will be used.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationBlankSuppressorCurrent
The electrical current supplied to the CationSuppressor module for CationBlank samples before conductivity measurement.
Default Calculation: This option will be calculated by multiplying flow rate, maximum buffer concentration and suppressor specific factor (2.94 for cation suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
CationBlankDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
BlankFrequency
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
BlankInjectionVolume
The physical quantity of each Blank to inject into the system. We recommend that injection volumes either be equal to the sample loop volume (25 uL) or less than or equal to one half of the sample loop volume (12.5 uL). Injection volumes between 12.5 and 25 uL may have lower accuracy and precision.
Pattern Description: Greater than or equal to 0 microliters and less than or equal to 25 microliters or Null.
BlankColumnTemperature
Default Calculation: Automatically set from ColumnTemperature or taken from the BlankGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
BlankGradientA
The composition of Buffer A within the flow for Blank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from BlankGradient option or implicitly determined from BlankGradientB, BlankGradientC, and BlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
BlankGradientB
The composition of Buffer B within the flow for Blank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from BlankGradient option or implicitly determined from BlankGradientA, BlankGradientC, and BlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
BlankGradientC
The composition of Buffer C within the flow for Blank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from BlankGradient option or implicitly determined from BlankGradientA, BlankGradientB, and BlankGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
BlankGradientD
The composition of Buffer D within the flow for Blank samples, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for BlankGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from BlankGradient option or implicitly determined from BlankGradientA, BlankGradientB, and BlankGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
BlankFlowRate
Default Calculation: Automatically set from Column, and FlowRate specified in the InjectionTable or taken from the method given in the BlankGradient option. If neither option is specified, the first value of FlowRate will be used
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 15*(Milliliter/Minute)]}..}) | Automatic) | Null
BlankGradientStart
A shorthand option to specify the starting BufferB concentration in the fluid flow of the instrument for Blank samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
BlankGradientEnd
A shorthand option to specify the final BufferB concentration in the fluid flow of the instrument for Blank samples.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
BlankGradientDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
BlankGradient
The buffer composition over time in the fluid flow for Blank samples. Specific parameters of an object can be overridden by specific options for BlankGradientA, BlankGradientB, BlankGradientC, and BlankGradientD.
Default Calculation: Automatically set to match all specified options related to BlankGradient (e.g. BlankGradientA, BlankGradientB, BlankGradientC, BlankGradientD, BlankFlowRate, and BlankGradientDuration).
Pattern Description: An object of type or subtype Object[Method, Gradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Gradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
BlankAbsorbanceWavelength
The physical properties of light passed through the flow for the UVVis Detector during Blank sample runs. Up to 4 separate wavelengths may be specified.
Default Calculation: Automatically set to 280 Nanometer if the UVVis detector is used in the protocol.
Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or list of one or more greater than or equal to 190 nanometers and less than or equal to 900 nanometers entries or Null.
Programmatic Pattern: ((RangeP[190*Nanometer, 900*Nanometer] | {RangeP[190*Nanometer, 900*Nanometer]..}) | Automatic) | Null
BlankAbsorbanceSamplingRate
Indicates the frequency of absorbance measurement for Blank samples. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set based on the number of wavelengths specified. Default BlankAbsorbanceSamplingRate is 10/Second for measurement at a single wavelength and 1/Second for measurements at multiple wavelengths.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
BlankElectrochemicalDetectionMode
Specifies the mode of operation for the electrochemical detector during Blank sample runs, including DC Amperometric Detection, Pulsed Amperometric Detection, and Integrated Pulsed Amperometric Detection. In DC Amperometric Detection, a constant voltage is applied. In contrast, Pulsed Amperometric Detections first apply a working potential followed by higher or lower potentials that are used for cleaning the electrode. Further, Integrated Amperometric Detection integrates current over a single potential whereas Integrated Pulsed Amperometric Detection integrates current over two or more potentials.
Default Calculation: Automatically set based on the BlankVoltageProfile or the BlankWaveformProfile specified. If neither option is specified, this option defaults to Pulsed Amperometric Detection.
Pattern Description: {DCAmperometricDetection, PulsedAmperometricDetection, IntegratedPulsedAmperometricDetection} or Null.
BlankReferenceElectrodeMode
A combination pH-Ag/AgCl reference electrode that can be used to either monitor the buffer pH ("pH" reference) or to serve as a cell reference electrode with a constant potential ("AgCl" reference).
Default Calculation: Automatically set from Waveform method object. If Waveform is not specified, this option defaults to AgCl.
BlankVoltageProfile
Default Calculation: Automatically set to 0.1 Volt for DCAmperometricDetection. If any other BlankElectrochemicalDetectionMode is used, this option will set to Null.
Pattern Description: Greater than or equal to -2.048 volts and less than or equal to 2.047 volts or list of one or more {Time, Voltage} entries or Null.
Programmatic Pattern: ((RangeP[-2.048*Volt, 2.047*Volt] | {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..}) | Automatic) | Null
BlankWaveformProfile
A series of time-dependent voltage setting (waveform) that will be repeated over the duration of the Blank sample analysis.
Default Calculation: Automatically set to repeat a single waveform defined for either Pulsed Amperometric Detection or Integrated Pulsed Amperometric Detection over the entire run.
Pattern Description: An object of type or subtype Object[Method, Waveform] or list of one or more {Time, Waveform} entries or list of one or more {Time, Waveform} entries or list of one or more {Waveform Time, Voltage, Interpolation, Integration} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Waveform]] | {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..} | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..}) | Automatic) | Null
BlankElectrochemicalSamplingRate
Indicates the frequency of amperometric measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set to 1/waveform duration if a single waveform is specified. If multiple waveforms are specified, this option is set to 1 over the longest waveform duration. If no waveform is specified, this option is set to 2/Second.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
BlankDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if electrochemical detector is used and Null if not.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
ColumnPrime
AnionColumnRefreshFrequency
Specify the frequency at which procedures to clear out and re-prime the AnionColumn will be inserted into the order of sample injections. If specified to a number, indicates how often the anion column prime runs will run among the samples; for example, if 5 input samples are measured and AnionColumnRefreshFrequency is 2, then a column prime will occur after the first two samples and again after the third and fourth.
Default Calculation: Set to Null when AnionInjectionTable option is specified (meaning that this option is inconsequential); otherwise, set to FirstAndLast (meaning initial anion column prime before the measurements and final anion column flush after measurements).
Pattern Description: Greater than or equal to 0 in increments of 1 or None, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | FirstAndLast | GradientChange) | GreaterEqualP[0, 1]) | Automatic) | Null
AnionColumnPrimeTemperature
Default Calculation: Automatically set from AnionColumnTemperature or taken from the AnionColumnPrimeGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
ColumnPrimeEluentGradient
The concentration of eluent, potassium hydroxide, that is automatically generated within the flow during AnionColumn Prime, defined for specific time points. Eluent is automatically generated in the flow path via electrolysis of deionized water. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnPrimeEluentGradient->{{0 Minute, 0 Millimolar},{30 Minute, 100 Millimolar}}, the eluent concentration in the flow will rise such that at 15 minutes, the concentration should be 50 Millimolar. Since eluent generator is only integrated in anion channel, this option is not applicable to cation column prime. Gradient for cation column prime can be set in CationColumnPrimeGradientA, CationColumnPrimeGradientB, CationColumnPrimeGradientC, CationColumnPrimeGradientD, and CationColumnPrimeGradient options.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or list of one or more {Time, Eluent Concentration} entries or Null.
Programmatic Pattern: ((RangeP[0*Millimolar, 100*Millimolar] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar]}..}) | Automatic) | Null
AnionColumnPrimeFlowRate
Default Calculation: Automatically set from AnionColumn and FlowRate specified in the AnionInjectionTable or taken from the method given in the AnionColumnPrimeGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionColumnPrimeStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for anion column prime.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionColumnPrimeEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for anion column prime.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionColumnPrimeDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionColumnPrimeGradient
The concentration of the eluent, potassium hydroxide, over time in the fluid flow for anion channel during anion column prime. Specific parameters of an object can be overridden by specific options for ColumnPrimeEluentGradient.
Default Calculation: Automatically set to match all specified options related to AnionColumnPrimeGradient (e.g. ColumnPrimeEluentGradient, AnionColumnPrimeGradientDuration, AnionColumnPrimeFlowRate). If none of the options is specified, the following default column prime gradient will be use: {{0 Minute, 100 Millimolar, 0.5 Milliliter/Minute}, {5 Minute, 100 Millimolar, 0.5 Milliliter/Minute}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Eluent Concentration, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionColumnPrimeSuppressorCurrent
Default Calculation: This option will be calculated by multiplying flow rate, maximum eluent concentration and suppressor specific factor (2.47 for anion suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
AnionColumnPrimeDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
CationColumnRefreshFrequency
Specify the frequency at which procedures to clear out and re-prime the CationColumn will be inserted into the order of sample injections. If specified to a number, indicates how often the cation column prime runs will run among the samples; for example, if 5 input samples are measured and CationColumnRefreshFrequency is 2, then a column prime will occur after the first two samples and again after the third and fourth.
Default Calculation: Set to Null when CationInjectionTable option is specified (meaning that this option is inconsequential); otherwise, set to FirstAndLast (meaning initial cation column prime before the measurements and final column flush after measurements).
Pattern Description: Greater than 0 in increments of 1 or None, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
CationColumnPrimeTemperature
Default Calculation: Automatically set from CationColumnTemperature or taken from the CationColumnPrimeGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
CationColumnPrimeGradientA
The composition of Buffer A within the flow during Column Prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnPrimeGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer A in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferA is only pumped into cation channel, this option is not applicable to anion column prime. Gradient for anion column prime can be set in ColumnPrimeEluentGradient and AnionColumnPrimeGradient options.
Default Calculation: Automatically set from CationColumnPrimeGradient option or implicitly determined from CationColumnPrimeGradientB, CationColumnPrimeGradientC, and CationColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnPrimeGradientB
The composition of Buffer B within the flow during Column Prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnPrimeGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer B in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferB is only pumped into cation channel, this option is not applicable to anion column prime. Gradient for anion column prime can be set in ColumnPrimeEluentGradient and AnionColumnPrimeGradient options.
Default Calculation: Automatically set from CationColumnPrimeGradient option or implicitly determined from CationColumnPrimeGradientA, CationColumnPrimeGradientC, and CationColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnPrimeGradientC
The composition of Buffer C within the flow during Column Prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnPrimeGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer C in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferC is only pumped into cation channel, this option is not applicable to anion column prime. Gradient for anion column prime can be set in ColumnPrimeEluentGradient and AnionColumnPrimeGradient options.
Default Calculation: Automatically set from CationColumnPrimeGradient option or implicitly determined from CationColumnPrimeGradientA, CationColumnPrimeGradientB, and CationColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnPrimeGradientD
The composition of Buffer D within the flow during Column Prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnPrimeGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer D in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferD is only pumped into cation channel, this option is not applicable to anion column prime. Gradient for anion column prime can be set in ColumnPrimeEluentGradient and AnionColumnPrimeGradient options.
Default Calculation: Automatically set from CationColumnPrimeGradient option or implicitly determined from CationColumnPrimeGradientA, CationColumnPrimeGradientB, and CationColumnPrimeGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnPrimeFlowRate
Default Calculation: Automatically set from CationColumn and FlowRate specified in the CationInjectionTable or taken from the method given in the CationColumnPrimeGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationColumnPrimeStart
A shorthand option to specify the starting buffer B concentration in the fluid flow of the instrument for cation column prime.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationColumnPrimeEnd
A shorthand option to specify the final buffer B concentration in the fluid flow of the instrument for cation column prime.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationColumnPrimeDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationColumnPrimeGradient
The buffer composition over time in the fluid flow during cation column prime. Specific parameters of an object can be overridden by specific options for CationColumnPrimeGradientA, CationColumnPrimeGradientB, CationColumnPrimeGradientC, and CationColumnPrimeGradientD.
Default Calculation: Automatically set to match all specified options related to CationColumnPrimeGradient (e.g. CationColumnPrimeGradientA, CationColumnPrimeGradientB, CationColumnPrimeGradientC, CationColumnPrimeGradientD, CationColumnPrimeGradientDuration, CationColumnPrimeFlowRate). If none of the options is specified, the following default column prime gradient will be used: {{0 Minute, 0 Percent, 0 Percent, 0 Percent, 100 Percent, 0.5 Milliliter/Minute}, {5 Minute, 0 Percent, 0 Percent, 0 Percent, 100 Percent, 0.5 Milliliter/Minute}, {5.1 Minute, 0 Percent, 0 Percent, 100 Percent, 0 Percent, 0.5 Milliliter/Minute}, {10 Minute, 0 Percent, 0 Percent, 100 Percent, 0 Percent, 0.5 Milliliter/Minute}, {10.1 Minute, 0 Percent, 100 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {15 Minute, 0 Percent, 100 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {15.1 Minute, 100 Percent, 0 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {20 Minute, 100 Percent, 0 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationColumnPrimeSuppressorCurrent
The electrical current supplied to the suppressor module in cation channel during cation column prime.
Default Calculation: This option will be calculated by multiplying flow rate, maximum buffer concentration and suppressor specific factor (2.94 for cation suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
CationColumnPrimeDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
ColumnRefreshFrequency
Specify the frequency at which procedures to clear out and re-prime the Column will be inserted into the order of sample injections. If specified to a number, indicates how often the anion column prime runs will run among the samples; for example, if 5 input samples are measured and ColumnRefreshFrequency is 2, then a column prime will occur after the first two samples and again after the third and fourth.
Pattern Description: Greater than 0 in increments of 1 or None, First, Last, FirstAndLast, or GradientChange or Null.
Programmatic Pattern: (((None | First | Last | FirstAndLast | GradientChange) | GreaterP[0, 1]) | Automatic) | Null
ColumnPrimeTemperature
Default Calculation: Automatically set from ColumnTemperature or taken from the ColumnPrimeGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
ColumnPrimeGradientA
The composition of Buffer A within the flow during column prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnPrimeGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnPrimeGradient option or implicitly determined from ColumnPrimeGradientB, ColumnPrimeGradientC, and ColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnPrimeGradientB
The composition of Buffer B within the flow during column prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnPrimeGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnPrimeGradient option or implicitly determined from ColumnPrimeGradientA, ColumnPrimeGradientC, and ColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnPrimeGradientC
The composition of Buffer C within the flow during column prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnPrimeGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnPrimeGradient option or implicitly determined from ColumnPrimeGradientA, ColumnPrimeGradientB, and ColumnPrimeGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnPrimeGradientD
The composition of Buffer D within the flow during column prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnPrimeGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnPrimeGradient option or implicitly determined from ColumnPrimeGradientA, ColumnPrimeGradientB, and ColumnPrimeGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnPrimeFlowRate
Default Calculation: Automatically set from Column, and FlowRate specified in the InjectionTable or taken from the method given in the ColumnPrimeGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
ColumnPrimeStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for column prime.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
ColumnPrimeEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for column prime.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
ColumnPrimeDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
ColumnPrimeGradient
The buffer composition over time in the fluid flow during column prime. Specific parameters of an object can be overridden by specific options for ColumnPrimeGradientA, ColumnPrimeGradientB, ColumnPrimeGradientC, and ColumnPrimeGradientD.
Default Calculation: Automatically set to match all specified options related to ColumnPrimeGradient (e.g. ColumnPrimeGradientA, ColumnPrimeGradientB, ColumnPrimeGradientC, ColumnPrimeGradientD, ColumnPrimeFlowRate, and ColumnPrimeGradientDuration).
Pattern Description: An object of type or subtype Object[Method, Gradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Gradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
ColumnPrimeAbsorbanceWavelength
The physical properties of light passed through the flow for the UVVis Detector during ColumnPrime. Up to 4 separate wavelengths may be specified.
Default Calculation: Automatically set to 280*Nanometer if the UVVis detector is used in the protocol and ColumnRefreshFrequency is not set to Null or None.
Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or list of one or more greater than or equal to 190 nanometers and less than or equal to 900 nanometers entries or Null.
Programmatic Pattern: ((RangeP[190*Nanometer, 900*Nanometer] | {RangeP[190*Nanometer, 900*Nanometer]..}) | Automatic) | Null
ColumnPrimeAbsorbanceSamplingRate
Indicates the frequency of absorbance measurement during ColumnPrime. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set based on the number of wavelengths specified. Default ColumnPrimeAbsorbanceSamplingRate is 10/Second for measurement at a single wavelength and 1/Second for measurements at multiple wavelengths.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
ColumnPrimeElectrochemicalDetectionMode
Specifies the mode of operation for the electrochemical detector during ColumnPrime, including DC Amperometric Detection, Pulsed Amperometric Detection, and Integrated Pulsed Amperometric Detection. In DC Amperometric Detection, a constant voltage is applied. In contrast, Pulsed Amperometric Detections first apply a working potential followed by higher or lower potentials that are used for cleaning the electrode. Further, Integrated Amperometric Detection integrates current over a single potential whereas Integrated Pulsed Amperometric Detection integrates current over two or more potentials.
Default Calculation: Automatically set based on the ColumnPrimeVoltageProfile or the ColumnPrimeWaveformProfile specified. If neither option is specified, this option defaults to Pulsed Amperometric Detection.
Pattern Description: {DCAmperometricDetection, PulsedAmperometricDetection, IntegratedPulsedAmperometricDetection} or Null.
ColumnPrimeReferenceElectrodeMode
A combination pH-Ag/AgCl reference electrode that can be used to either monitor the buffer pH ("pH" reference) or to serve as a cell reference electrode with a constant potential ("AgCl" reference).
Default Calculation: Automatically set from Waveform method object. If Waveform is not specified, this option defaults to AgCl.
ColumnPrimeVoltageProfile
Default Calculation: Automatically set to 0.1 Volt for DCAmperometricDetection. If any other ColumnPrimeElectrochemicalDetectionMode is used, this option will set to Null.
Pattern Description: Greater than or equal to -2.048 volts and less than or equal to 2.047 volts or list of one or more {Time, Voltage} entries or Null.
Programmatic Pattern: ((RangeP[-2.048*Volt, 2.047*Volt] | {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..}) | Automatic) | Null
ColumnPrimeWaveformProfile
A series of time-dependent voltage setting (waveform) that will be repeated over the duration of ColumnPrime.
Default Calculation: Automatically set to repeat a single waveform defined for either Pulsed Amperometric Detection or Integrated Pulsed Amperometric Detection over the entire run.
Pattern Description: An object of type or subtype Object[Method, Waveform] or list of one or more {Time, Waveform} entries or list of one or more {Time, Waveform} entries or list of one or more {Waveform Time, Voltage, Interpolation, Integration} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Waveform]] | {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..} | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..}) | Automatic) | Null
ColumnPrimeElectrochemicalSamplingRate
Indicates the frequency of amperometric measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set to 1/waveform duration if a single waveform is specified. If multiple waveforms are specified, this option is set to 1 over the longest waveform duration. If no waveform is specified, this option is set to 2/Second.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
ColumnPrimeDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if electrochemical detector is used.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
ColumnFlush
AnionColumnFlushTemperature
Default Calculation: Automatically set from AnionColumnTemperature or taken from the AnionColumnFlushGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
ColumnFlushEluentGradient
The concentration of eluent, potassium hydroxide, that is automatically generated within the flow during Column Flush, defined for specific time points. Eluent is automatically generated in the flow path via electrolysis of deionized water. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnFlushEluentGradient->{{0 Minute, 0 Millimolar},{30 Minute, 100 Millimolar}}, the eluent concentration in the flow will rise such that at 15 minutes, the concentration should be 50 Millimolar. Since eluent generator is only integrated in anion channel, this option is not applicable to cation column flush. Gradient for cation column flush can be set in CationColumnFlushGradientA, CationColumnFlushGradientB, CationColumnFlushGradientC, CationColumnFlushGradientD, and CationColumnFlushGradient options.
Default Calculation: Automatically set to match all specified options related to AnionColumnFlushGradient (e.g. ColumnFlushEluentGradient, AnionColumnFlushGradientDuration, AnionColumnFlushFlowRate).
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or list of one or more {Time, Eluent Concentration} entries or Null.
Programmatic Pattern: ((RangeP[0*Millimolar, 100*Millimolar] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar]}..}) | Automatic) | Null
AnionColumnFlushFlowRate
Default Calculation: Automatically set from AnionColumn and FlowRate specified in the AnionInjectionTable or taken from the method given in the AnionColumnFlushGradient option. If neither option is specified, the smaller flow rate between the minumum flow rate of the resolved AnionColumn and that of the resolved Instrument will be used.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionColumnFlushStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for anion column flush.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionColumnFlushEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for anion column flush.
Pattern Description: Greater than or equal to 0 millimolar and less than or equal to 100 millimolar or Null.
AnionColumnFlushDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
AnionColumnFlushGradient
The concentration of the eluent, potassium hydroxide, over time in the fluid flow for anion channel during anion column flush. Specific parameters of an object can be overridden by specific options for ColumnFlushEluentGradient.
Default Calculation: Automatically set to match all specified options related to AnionColumnFlushGradient (e.g. ColumnFlushEluentGradient, AnionColumnFlushGradientDuration, AnionColumnFlushFlowRate). If none of the options is specified, the following column flush gradient will be used: {{0 Minute, 100 Millimolar, 0.5 Milliliter/Minute}, {5 Minute, 100 Millimolar, 0.5 Milliliter/Minute}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Eluent Concentration, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Millimolar, 100*Millimolar], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
AnionColumnFlushSuppressorCurrent
Default Calculation: This option will be calculated by multiplying flow rate, maximum eluent concentration and suppressor specific factor (2.47 for anion suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
AnionColumnFlushDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
CationColumnFlushTemperature
Default Calculation: Automatically set from CationColumnTemperature or taken from the CationColumnFlushGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
CationColumnFlushGradientA
The composition of Buffer A within the flow during ColumnFlush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnFlushGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer A in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferA is only pumped into cation channel, this option is not applicable to anion column flush. Gradient for anion column flush can be set in ColumnFlushEluentGradient and AnionColumnFlushGradient options.
Default Calculation: Automatically set from CationColumnFlushGradient option or implicitly determined from CationColumnFlushGradientB, CationColumnFlushGradientC, and CationColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnFlushGradientB
The composition of Buffer B within the flow during ColumnFlush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnFlushGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer B in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferB is only pumped into cation channel, this option is not applicable to anion column flush. Gradient for anion column flush can be set in ColumnFlushEluentGradient and AnionColumnFlushGradient options.
Default Calculation: Automatically set from CationColumnFlushGradient option or implicitly determined from CationColumnFlushGradientA, CationColumnFlushGradientC, and CationColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnFlushGradientC
The composition of Buffer C within the flow during ColumnFlush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnFlushGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer C in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferC is only pumped into cation channel, this option is not applicable to anion column flush. Gradient for anion column flush can be set in ColumnFlushEluentGradient and AnionColumnFlushGradient options.
Default Calculation: Automatically set from CationColumnFlushGradient option or implicitly determined from CationColumnFlushGradientA, CationColumnFlushGradientB, and CationColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnFlushGradientD
The composition of Buffer D within the flow during ColumnFlush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for CationColumnFlushGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of Buffer D in the flow will rise such that at 15 minutes, the composition should be 50*Percent. Since BufferD is only pumped into cation channel, this option is not applicable to anion column flush. Gradient for anion column flush can be set in ColumnFlushEluentGradient and AnionColumnFlushGradient options.
Default Calculation: Automatically set from CationColumnFlushGradient option or implicitly determined from CationColumnFlushGradientA, CationColumnFlushGradientB, and CationColumnFlushGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
CationColumnFlushFlowRate
Default Calculation: Automatically set from CationColumn and FlowRate specified in the CationInjectionTable or taken from the method given in the CationColumnFlushGradient option.
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationColumnFlushStart
A shorthand option to specify the starting buffer B concentration in the fluid flow of the instrument for cation column flush.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationColumnFlushEnd
A shorthand option to specify the final buffer B concentration in the fluid flow of the instrument for cation column flush.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
CationColumnFlushDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
CationColumnFlushGradient
The buffer composition over time in the fluid flow during cation column flush. Specific parameters of an object can be overridden by specific options for CationColumnFlushGradientA, CationColumnFlushGradientB, CationColumnFlushGradientC, and CationColumnFlushGradientD.
Default Calculation: Automatically set to match all specified options related to CationColumnFlushGradient (e.g. CationColumnFlushGradient, CationColumnFlushGradientB, CationColumnFlushGradientC, CationColumnFlushGradientD, CationColumnFlushGradientDuration, CationColumnFlushFlowRate). If none of the options is specified, the following column flush gradient will be used: {{0 Minute, 0 Percent, 0 Percent, 0 Percent, 100 Percent, 0.5 Milliliter/Minute}, {5 Minute, 0 Percent, 0 Percent, 0 Percent, 100 Percent, 0.5 Milliliter/Minute}, {5.1 Minute, 0 Percent, 0 Percent, 100 Percent, 0 Percent, 0.5 Milliliter/Minute}, {10 Minute, 0 Percent, 0 Percent, 100 Percent, 0 Percent, 0.5 Milliliter/Minute}, {10.1 Minute, 0 Percent, 100 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {15 Minute, 0 Percent, 100 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {15.1 Minute, 100 Percent, 0 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}, {20 Minute, 100 Percent, 0 Percent, 0 Percent, 0 Percent, 0.5 Milliliter/Minute}}.
Pattern Description: An object of type or subtype Object[Method, IonChromatographyGradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, IonChromatographyGradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
CationColumnFlushSuppressorCurrent
Default Calculation: This option will be calculated by multiplying flow rate, maximum buffer concentration and suppressor specific factor (2.94 for cation suppressor).
Pattern Description: Greater than or equal to 0 milliamperes and less than or equal to 150 milliamperes or Null.
CationColumnFlushDetectionTemperature
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
ColumnFlushTemperature
Default Calculation: Automatically set from ColumnTemperature or taken from the ColumnFlushGradient option.
Pattern Description: Ambient or greater than or equal to 10 degrees Celsius and less than or equal to 70 degrees Celsius or Null.
ColumnFlushGradientA
The composition of Buffer A within the flow during column prime, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnFlushGradientA->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferA in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnFlushGradient option or implicitly determined from ColumnFlushGradientB, ColumnFlushGradientC, and ColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer A Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnFlushGradientB
The composition of Buffer B within the flow during column flush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnFlushGradientB->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferB in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnFlushGradient option or implicitly determined from ColumnFlushGradientA, ColumnFlushGradientC, and ColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer B Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnFlushGradientC
The composition of Buffer C within the flow during column flush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnFlushGradientC->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferC in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnFlushGradient option or implicitly determined from ColumnFlushGradientA, ColumnFlushGradientB, and ColumnFlushGradientD options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer C Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnFlushGradientD
The composition of Buffer D within the flow during column flush, defined for specific time points. The composition is linearly interpolated for the intervening periods between the defined time points. For example for ColumnFlushGradientD->{{0 Minute, 0 Percent},{30 Minute, 100 Percent}}, the percentage of BufferD in the flow will rise such that at 15 minutes, the composition should be 50*Percent.
Default Calculation: Automatically set from ColumnFlushGradient option or implicitly determined from ColumnFlushGradientA, ColumnFlushGradientB, and ColumnFlushGradientC options such that composition of BufferA, B, C, and D sums up to 100 Percent throughout the gradient.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or list of one or more {Time, Buffer D Composition} entries or Null.
Programmatic Pattern: ((RangeP[0*Percent, 100*Percent] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent]}..}) | Automatic) | Null
ColumnFlushFlowRate
Default Calculation: Automatically set from Column, and FlowRate specified in the InjectionTable or taken from the method given in the ColumnFlushGradient option. If neither option is specified, the first value of FlowRate will be used
Pattern Description: Greater than or equal to 0 milliliters per minute and less than or equal to 10 milliliters per minute or list of one or more {Time, Flow Rate} entries or Null.
Programmatic Pattern: ((RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
ColumnFlushStart
A shorthand option to specify the starting eluent concentration in the fluid flow of the instrument for column flush.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
ColumnFlushEnd
A shorthand option to specify the final eluent concentration in the fluid flow of the instrument for column flush.
Pattern Description: Greater than or equal to 0 percent and less than or equal to 100 percent or Null.
ColumnFlushDuration
Pattern Description: Greater than or equal to 0 minutes and less than or equal to 4320 minutes or Null.
ColumnFlushGradient
The buffer composition over time in the fluid flow during column flush. Specific parameters of an object can be overridden by specific options for ColumnFlushGradientA, ColumnFlushGradientB, ColumnFlushGradientC, and ColumnFlushGradientD.
Default Calculation: Automatically set to match all specified options related to ColumnFlushGradient (e.g. ColumnFlushGradientA, ColumnFlushGradientB, ColumnFlushGradientC, ColumnFlushGradientD, ColumnFlushFlowRate, and ColumnFlushGradientDuration).
Pattern Description: An object of type or subtype Object[Method, Gradient] or list of one or more {Time, Buffer A Composition, Buffer B Composition, Buffer C Composition, Buffer D Composition, Flow Rate} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Gradient]] | {{RangeP[0*Minute, 4320*Minute], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*Percent, 100*Percent], RangeP[0*(Milliliter/Minute), 10*(Milliliter/Minute)]}..}) | Automatic) | Null
ColumnFlushAbsorbanceWavelength
The physical properties of light passed through the flow for the UVVis Detector during ColumnFlush. Up to 4 separate wavelengths may be specified.
Default Calculation: Automatically set to 280*Nanometer if the UVVis detector is used in the protocol and ColumnRefreshFrequency is not set to Null or None.
Pattern Description: Greater than or equal to 190 nanometers and less than or equal to 900 nanometers or list of one or more greater than or equal to 190 nanometers and less than or equal to 900 nanometers entries or Null.
Programmatic Pattern: ((RangeP[190*Nanometer, 900*Nanometer] | {RangeP[190*Nanometer, 900*Nanometer]..}) | Automatic) | Null
ColumnFlushAbsorbanceSamplingRate
Indicates the frequency of absorbance measurement during ColumnFlush. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set based on the number of wavelengths specified. Default ColumnFlushAbsorbanceSamplingRate is 10/Second for measurement at a single wavelength and 1/Second for measurements at multiple wavelengths.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
ColumnFlushElectrochemicalDetectionMode
Specifies the mode of operation for the electrochemical detector during ColumnFlush, including DC Amperometric Detection, Pulsed Amperometric Detection, and Integrated Pulsed Amperometric Detection. In DC Amperometric Detection, a constant voltage is applied. In contrast, Pulsed Amperometric Detections first apply a working potential followed by higher or lower potentials that are used for cleaning the electrode. Further, Integrated Amperometric Detection integrates current over a single potential whereas Integrated Pulsed Amperometric Detection integrates current over two or more potentials.
Default Calculation: Automatically set based on the ColumnFlushVoltageProfile or the ColumnFlushWaveformProfile specified. If neither option is specified, this option defaults to Pulsed Amperometric Detection.
Pattern Description: {DCAmperometricDetection, PulsedAmperometricDetection, IntegratedPulsedAmperometricDetection} or Null.
ColumnFlushReferenceElectrodeMode
A combination pH-Ag/AgCl reference electrode that can be used to either monitor the buffer pH ("pH" reference) or to serve as a cell reference electrode with a constant potential ("AgCl" reference).
Default Calculation: Automatically set from Waveform method object. If Waveform is not specified, this option defaults to AgCl.
ColumnFlushVoltageProfile
Default Calculation: Automatically set to 0.1 Volt for DCAmperometricDetection. If any other ColumnFlushElectrochemicalDetectionMode is used, this option will set to Null.
Pattern Description: Greater than or equal to -2.048 volts and less than or equal to 2.047 volts or list of one or more {Time, Voltage} entries or Null.
Programmatic Pattern: ((RangeP[-2.048*Volt, 2.047*Volt] | {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..}) | Automatic) | Null
ColumnFlushWaveformProfile
A series of time-dependent voltage setting (waveform) that will be repeated over the duration of ColumnFlush.
Default Calculation: Automatically set to repeat a single waveform defined for either Pulsed Amperometric Detection or Integrated Pulsed Amperometric Detection over the entire run.
Pattern Description: An object of type or subtype Object[Method, Waveform] or list of one or more {Time, Waveform} entries or list of one or more {Time, Waveform} entries or list of one or more {Waveform Time, Voltage, Interpolation, Integration} entries or Null.
Programmatic Pattern: ((ObjectP[Object[Method, Waveform]] | {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..} | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..}) | Automatic) | Null
ColumnFlushElectrochemicalSamplingRate
Indicates the frequency of amperometric measurement. Lower values will be less susceptible to noise but will record less frequently across time.
Default Calculation: Automatically set to 1/waveform duration if a single waveform is specified. If multiple waveforms are specified, this option is set to 1 over the longest waveform duration. If no waveform is specified, this option is set to 2/Second.
Pattern Description: Greater than or equal to 0.2 reciprocal seconds and less than or equal to 100 reciprocal seconds or Null.
ColumnFlushDetectionTemperature
Default Calculation: This option will automatically be set to Ambient if electrochemical detector is used and Null if not.
Pattern Description: Ambient or greater than or equal to 15 degrees Celsius and less than or equal to 35 degrees Celsius or Null.
Sample Parameters
AnionInjectionTable
The order of AnionSamples, AnionStandard, and AnionBlank sample loading into the anion flow path of the Instrument during measurement. Also includes the flushing and priming of the AnionColumn.
Default Calculation: Determined to the order of AnionSamples articulated. AnionStandard and AnionBlank samples are inserted based on the determination of AnionStandardFrequency and AnionBlankFrequency. For example, AnionStandardFrequency->FirstAndLast and AnionBlankFrequency->Null result in AnionStandard samples being injected first, then the AnionSamples, and then the AnionStandard again at the end.
Pattern Description: List of one or more {Type, Analysis Channel, Gradient} or {Type, Anion Samples, Analysis Channel, Injection Volume, Gradient} entries or Null.
Programmatic Pattern: ({({InjectionTableSampleTypeP, Alternatives[ObjectP[{Model[Sample], Object[Sample]}] | _String], AnionChannel | Automatic, RangeP[0*Microliter, 10*Microliter] | Automatic, ObjectP[Object[Method, IonChromatographyGradient]] | Automatic} | {InjectionTableCleaningTypeP, AnionChannel | Automatic, ObjectP[Object[Method, IonChromatographyGradient]] | Automatic})..} | Automatic) | Null
CationInjectionTable
The order of CationSamples, CationStandard, and CationBlank sample loading into the cation flow path of the Instrument during measurement. Also includes the flushing and priming of the CaitonColumn.
Default Calculation: Determined to the order of CationSamples articulated. CationStandard and CationBlank samples are inserted based on the determination of CationStandardFrequency and CationBlankFrequency. For example, CationStandardFrequency->FirstAndLast and CationBlankFrequency->Null result in CationStandard samples being injected first, then the CationSamples, and then the CationStandard again at the end.
Pattern Description: List of one or more {Type, Analysis Channel, Gradient} or {Type, Cation Samples, Analysis Channel, Injection Volume, Gradient} entries or Null.
Programmatic Pattern: ({({InjectionTableSampleTypeP, Alternatives[ObjectP[{Model[Sample], Object[Sample]}] | _String], CationChannel | Automatic, RangeP[0*Microliter, 10*Microliter] | Automatic, ObjectP[Object[Method, IonChromatographyGradient]] | Automatic} | {InjectionTableCleaningTypeP, CationChannel | Automatic, ObjectP[Object[Method, IonChromatographyGradient]] | Automatic})..} | Automatic) | Null
ElectrochemicalInjectionTable
The order of Samples, Standard, and Blank sample loading into the flow path of the Instrument during measurement. Also includes the flushing and priming of the Column.
Default Calculation: Determined to the order of input samples articulated. Standard and Blank samples are inserted based on the determination of StandardFrequency and BlankFrequency. For example, StandardFrequency->FirstAndLast and BlankFrequency->Null result in Standard samples injected first, then the samples, and then the Standard sample again at the end.
Pattern Description: List of one or more {Type, Gradient, Waveform Profile, Voltage Profile} or {Type, Samples, Injection Volume, Gradient, Waveform Profile, Voltage Profile} entries or Null.
Programmatic Pattern: ({({InjectionTableSampleTypeP, (ObjectP[{Model[Sample], Object[Sample]}] | _String) | Automatic, RangeP[0*Microliter, 25*Microliter] | Automatic, ObjectP[Object[Method, Gradient]] | Automatic, ObjectP[Object[Method, Waveform]] | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..} | (Automatic | Null), {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..} | RangeP[-2.048*Volt, 2.047*Volt] | (Automatic | Null)} | {InjectionTableCleaningTypeP, ObjectP[Object[Method, Gradient]] | Automatic, ObjectP[Object[Method, Waveform]] | {{RangeP[0*Minute, 4320*Minute], ObjectP[Object[Method, Waveform]]}..} | {{RangeP[0*Minute, 4320*Minute], {{RangeP[0*Second, 2*Second], RangeP[-2.048*Volt, 2.047*Volt], BooleanP, BooleanP}..}}..} | (Automatic | Null), {{RangeP[0*Minute, 4320*Minute], RangeP[-2.048*Volt, 2.047*Volt]}..} | RangeP[-2.048*Volt, 2.047*Volt] | (Automatic | Null)})..} | Automatic) | Null
SampleTemperature
Pattern Description: Ambient or greater than or equal to 4 degrees Celsius and less than or equal to 60 degrees Celsius.
NeedleWashSolution
Post Experiment
SamplesInStorageCondition
The non-default conditions under which the SamplesIn of this experiment should be stored after the protocol is completed. If left unset, SamplesIn will be stored according to their current StorageCondition.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
Model Input
PreparedModelAmount
Indicates the amount of a Model[Sample] specified as input to the experiment function that will be prepared in the PreparedModelContainer. When set to All and the input model sample is not preparable, the entire amount of the input model sample that comes from one of the Products is prepared. The selected product must have both Amount and DefaultContainerModel populated, and it must not be a KitProduct. When set to All and the input model is preparable such as water, 1 Milliliter of the input model sample is prepared.
Programmatic Pattern: ((Null | (RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All)) | Automatic) | Null
PreparedModelContainer
Indicates the container in which a Model[Sample] specified as input to the experiment function will be prepared.
Default Calculation: If PreparedModelAmount is set to All and the input model has a product associated with both Amount and DefaultContainerModel populated, automatically set to the DefaultContainerModel value in the product. Otherwise, automatically set to Model[Container, Plate, "96-well 2mL Deep Well Plate"].
Sample Prep Options
Sample Preparation
PreparatoryUnitOperations
Specifies a sequence of transferring, aliquoting, consolidating, or mixing of new or existing samples before the main experiment. These prepared samples can be used in the main experiment by referencing their defined name. For more information, please reference the documentation for ExperimentSamplePreparation.
Pattern Description: List of one or more unit Operation ManualSamplePreparation or RoboticSamplePreparation or unit Operation must match SamplePreparationP entries or Null.
Programmatic Pattern: {((ManualSamplePreparationMethodP | RoboticSamplePreparationMethodP) | SamplePreparationP)..} | Null
Preparatory Incubation
Incubate
Indicates if the SamplesIn should be incubated at a fixed temperature prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Incubation options are set. Otherwise, resolves to False.
IncubationTemperature
Temperature at which the SamplesIn should be incubated for the duration of the IncubationTime prior to starting the experiment.
Pattern Description: Ambient or greater than or equal to -20 degrees Celsius and less than or equal to 500 degrees Celsius or Null.
Programmatic Pattern: ((Ambient | RangeP[$MinIncubationTemperature, $MaxIncubationTemperature]) | Automatic) | Null
IncubationTime
Duration for which SamplesIn should be incubated at the IncubationTemperature, prior to starting the experiment.
Mix
Default Calculation: Automatically resolves to True if any Mix related options are set. Otherwise, resolves to False.
MixType
Default Calculation: Automatically resolves based on the container of the sample and the Mix option.
Pattern Description: Roll, Vortex, Sonicate, Pipette, Invert, Stir, Shake, Homogenize, Swirl, Disrupt, or Nutate or Null.
MixUntilDissolved
Indicates if the mix should be continued up to the MaxIncubationTime or MaxNumberOfMixes (chosen according to the mix Type), in an attempt dissolve any solute. Any mixing/incubation will occur prior to starting the experiment.
Default Calculation: Automatically resolves to True if MaxIncubationTime or MaxNumberOfMixes is set.
MaxIncubationTime
Maximum duration of time for which the samples will be mixed while incubated in an attempt to dissolve any solute, if the MixUntilDissolved option is chosen. This occurs prior to starting the experiment.
Default Calculation: Automatically resolves based on MixType, MixUntilDissolved, and the container of the given sample.
IncubationInstrument
Default Calculation: Automatically resolves based on the options Mix, Temperature, MixType and container of the sample.
Pattern Description: An object of type or subtype Model[Instrument, Roller], Model[Instrument, OverheadStirrer], Model[Instrument, Vortex], Model[Instrument, Shaker], Model[Instrument, BottleRoller], Model[Instrument, Roller], Model[Instrument, Sonicator], Model[Instrument, HeatBlock], Model[Instrument, Homogenizer], Model[Instrument, Disruptor], Model[Instrument, Nutator], Model[Instrument, Thermocycler], Model[Instrument, EnvironmentalChamber], Model[Instrument, Pipette], Object[Instrument, Roller], Object[Instrument, OverheadStirrer], Object[Instrument, Vortex], Object[Instrument, Shaker], Object[Instrument, BottleRoller], Object[Instrument, Roller], Object[Instrument, Sonicator], Object[Instrument, HeatBlock], Object[Instrument, Homogenizer], Object[Instrument, Disruptor], Object[Instrument, Nutator], Object[Instrument, Thermocycler], Object[Instrument, EnvironmentalChamber], or Object[Instrument, Pipette] or Null.
AnnealingTime
Minimum duration for which the SamplesIn should remain in the incubator allowing the system to settle to room temperature after the IncubationTime has passed but prior to starting the experiment.
IncubateAliquotContainer
The desired type of container that should be used to prepare and house the incubation samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
IncubateAliquotDestinationWell
The desired position in the corresponding IncubateAliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
IncubateAliquot
The amount of each sample that should be transferred from the SamplesIn into the IncubateAliquotContainer when performing an aliquot before incubation.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
Preparatory Centrifugation
Centrifuge
Indicates if the SamplesIn should be centrifuged prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Centrifuge options are set. Otherwise, resolves to False.
CentrifugeInstrument
Pattern Description: An object of type or subtype Model[Instrument, Centrifuge] or Object[Instrument, Centrifuge] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, Centrifuge], Object[Instrument, Centrifuge]}] | Automatic) | Null
CentrifugeIntensity
The rotational speed or the force that will be applied to the samples by centrifugation prior to starting the experiment.
Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
CentrifugeTime
CentrifugeTemperature
The temperature at which the centrifuge chamber should be held while the samples are being centrifuged prior to starting the experiment.
Pattern Description: Ambient or greater than or equal to -10 degrees Celsius and less than or equal to 40 degrees Celsius or Null.
CentrifugeAliquotContainer
The desired type of container that should be used to prepare and house the centrifuge samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
CentrifugeAliquotDestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
CentrifugeAliquot
The amount of each sample that should be transferred from the SamplesIn into the CentrifugeAliquotContainer when performing an aliquot before centrifugation.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
Preparatory Filtering
Filtration
Indicates if the SamplesIn should be filter prior to starting the experiment or any aliquoting. Sample Preparation occurs in the order of Incubation, Centrifugation, Filtration, and then Aliquoting (if specified).
Default Calculation: Resolves to True if any of the corresponding Filter options are set. Otherwise, resolves to False.
FiltrationType
Default Calculation: Will automatically resolve to a filtration type appropriate for the volume of sample being filtered.
FilterInstrument
Default Calculation: Will automatically resolved to an instrument appropriate for the filtration type.
Pattern Description: An object of type or subtype Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], or Object[Instrument, SyringePump] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, FilterBlock], Object[Instrument, FilterBlock], Model[Instrument, PeristalticPump], Object[Instrument, PeristalticPump], Model[Instrument, VacuumPump], Object[Instrument, VacuumPump], Model[Instrument, Centrifuge], Object[Instrument, Centrifuge], Model[Instrument, SyringePump], Object[Instrument, SyringePump]}] | Automatic) | Null
Filter
The filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Will automatically resolve to a filter appropriate for the filtration type and instrument.
Pattern Description: An object of type or subtype Model[Container, Plate, Filter], Model[Container, Vessel, Filter], or Model[Item, Filter] or Null.
Programmatic Pattern: (ObjectP[{Model[Container, Plate, Filter], Model[Container, Vessel, Filter], Model[Item, Filter]}] | Automatic) | Null
FilterMaterial
The membrane material of the filter that should be used to remove impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Resolves to an appropriate filter material for the given sample is Filtration is set to True.
Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, AgaroseResin, CobaltResin, Silica, HLB, or AnoporeAlumina or Null.
PrefilterMaterial
The material from which the prefilter filtration membrane should be made of to remove impurities from the SamplesIn prior to starting the experiment.
Pattern Description: Cellulose, Cotton, Polyethylene, Polypropylene, PTFE, Nylon, PES, PLUS, PVDF, GlassFiber, GHP, UHMWPE, EPDM, DuraporePVDF, GxF, ZebaDesaltingResin, NickelResin, AgaroseResin, CobaltResin, Silica, HLB, or AnoporeAlumina or Null.
FilterPoreSize
The pore size of the filter that should be used when removing impurities from the SamplesIn prior to starting the experiment.
Default Calculation: Resolves to an appropriate filter pore size for the given sample is Filtration is set to True.
Pattern Description: 0.008 micrometers, 0.02 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
PrefilterPoreSize
The pore size of the filter; all particles larger than this should be removed during the filtration.
Pattern Description: 0.008 micrometers, 0.02 micrometers, 0.1 micrometers, 0.2 micrometers, 0.22 micrometers, 0.45 micrometers, 1. micrometer, 1.1 micrometers, 2.5 micrometers, 6. micrometers, 20. micrometers, 30. micrometers, or 100. micrometers or Null.
FilterSyringe
Default Calculation: Resolves to an syringe appropriate to the volume of sample being filtered, if Filtration is set to True.
Pattern Description: An object of type or subtype Model[Container, Syringe] or Object[Container, Syringe] or a prepared sample or Null.
Programmatic Pattern: ((ObjectP[{Model[Container, Syringe], Object[Container, Syringe]}] | _String) | Automatic) | Null
FilterHousing
The filter housing that should be used to hold the filter membrane when filtration is performed using a standalone filter membrane.
Default Calculation: Resolve to an housing capable of holding the size of the membrane being used, if filter with Membrane FilterType is being used and Filtration is set to True.
Pattern Description: An object of type or subtype Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], or Object[Instrument, FilterBlock] or Null.
Programmatic Pattern: (ObjectP[{Model[Instrument, FilterHousing], Object[Instrument, FilterHousing], Model[Instrument, FilterBlock], Object[Instrument, FilterBlock]}] | Automatic) | Null
FilterIntensity
Default Calculation: Will automatically resolve to 2000 GravitationalAcceleration if FiltrationType is Centrifuge and Filtration is True.
Pattern Description: Greater than 0 revolutions per minute or greater than 0 standard accelerations due to gravity on the surface of the earth or Null.
Programmatic Pattern: ((GreaterP[0*RPM] | GreaterP[0*GravitationalAcceleration]) | Automatic) | Null
FilterTime
Default Calculation: Will automatically resolve to 5 Minute if FiltrationType is Centrifuge and Filtration is True.
FilterTemperature
The temperature at which the centrifuge chamber will be held while the samples are being centrifuged during filtration.
Default Calculation: Will automatically resolve to 22 Celsius if FiltrationType is Centrifuge and Filtration is True.
FilterContainerOut
The desired container filtered samples should be produced in or transferred into by the end of filtration, with indices indicating grouping of samples in the same plates, if desired.
Default Calculation: Automatically set as the PreferredContainer for the Volume of the sample. For plates, attempts to fill all wells of a single plate with the same model before using another one.
Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or {Index, Container} or Null.
Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | {GreaterEqualP[1, 1] | Automatic, (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
FilterAliquotDestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
FilterAliquotContainer
The desired type of container that should be used to prepare and house the filter samples which should be used in lieu of the SamplesIn for the experiment.
Programmatic Pattern: ((ObjectP[Model[Container]] | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | Automatic}) | Automatic) | Null
FilterAliquot
The amount of each sample that should be transferred from the SamplesIn into the FilterAliquotContainer when performing an aliquot before filtration.
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container.
Pattern Description: All or greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
FilterSterile
Default Calculation: Resolve to False if Filtration is indicated. If sterile filtration is desired, this option must manually be set to True.
Aliquoting
Aliquot
Indicates if aliquots should be taken from the SamplesIn and transferred into new AliquotSamples used in lieu of the SamplesIn for the experiment. Note that if NumberOfReplicates is specified this indicates that the input samples will also be aliquoted that number of times. Note that Aliquoting (if specified) occurs after any Sample Preparation (if specified).
AliquotAmount
Default Calculation: Automatically set as the smaller between the current sample volume and the maximum volume of the destination container if a liquid, or the current Mass or Count if a solid or counted item, respectively.
Programmatic Pattern: ((RangeP[1*Microliter, 20*Liter] | RangeP[1*Milligram, 20*Kilogram] | GreaterP[0*Unit, 1*Unit] | GreaterP[0., 1.] | All) | Automatic) | Null
TargetConcentration
The desired final concentration of analyte in the AliquotSamples after dilution of aliquots of SamplesIn with the ConcentratedBuffer and BufferDiluent which should be used in lieu of the SamplesIn for the experiment.
TargetConcentrationAnalyte
Default Calculation: Automatically set to the first value in the Analytes field of the input sample, or, if not populated, to the first analyte in the Composition field of the input sample, or if none exist, the first identity model of any kind in the Composition field.
Pattern Description: An object of type or subtype Model[Molecule], Model[Molecule, cDNA], Model[Molecule, Oligomer], Model[Molecule, Transcript], Model[Molecule, Protein], Model[Molecule, Protein, Antibody], Model[Molecule, Carbohydrate], Model[Molecule, Polymer], Model[Resin], Model[Resin, SolidPhaseSupport], Model[Lysate], Model[ProprietaryFormulation], Model[Virus], Model[Cell], Model[Cell, Mammalian], Model[Cell, Bacteria], Model[Cell, Yeast], Model[Tissue], Model[Material], or Model[Species] or Null.
AssayVolume
Default Calculation: Automatically determined based on Volume and TargetConcentration option values.
Pattern Description: Greater than or equal to 1 microliter and less than or equal to 20 liters or Null.
ConcentratedBuffer
The concentrated buffer which should be diluted by the BufferDilutionFactor in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
BufferDilutionFactor
The dilution factor by which the concentrated buffer should be diluted in the final solution (i.e., the combination of the sample, ConcentratedBuffer, and BufferDiluent). The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: If ConcentratedBuffer is specified, automatically set to the ConcentratedBufferDilutionFactor of that sample; otherwise, set to Null.
BufferDiluent
The buffer used to dilute the aliquot sample such that ConcentratedBuffer is diluted by BufferDilutionFactor in the final solution. The ConcentratedBuffer and BufferDiluent will be combined and then mixed with the sample, where the combined volume of these buffers is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is specified; otherwise, resolves to Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AssayBuffer
The buffer that should be added to any aliquots requiring dilution, where the volume of this buffer added is the difference between the AliquotAmount and the total AssayVolume.
Default Calculation: Automatically resolves to Model[Sample, "Milli-Q water"] if ConcentratedBuffer is not specified; otherwise, resolves to Null.
Pattern Description: An object of type or subtype Model[Sample] or Object[Sample] or a prepared sample or Null.
AliquotSampleStorageCondition
The non-default conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Pattern Description: {AmbientStorage, EnclosedAmbientStorage, Refrigerator, Freezer, DeepFreezer, CryogenicStorage, YeastIncubation, YeastShakingIncubation, BacterialIncubation, BacterialShakingIncubation, MammalianIncubation, ViralIncubation, CrystalIncubation, AcceleratedTesting, IntermediateTesting, LongTermTesting, UVVisLightTesting} or Disposal or Null.
DestinationWell
The desired position in the corresponding AliquotContainer in which the aliquot samples will be placed.
Default Calculation: Automatically resolves to A1 in containers with only one position. For plates, fills wells in the order provided by the function AllWells.
Pattern Description: Any well from A1 to H12 or list of one or more any well from A1 to H12 or any well from A1 to H12 entries or Null.
Programmatic Pattern: ((WellPositionP | {((Automatic | Null) | WellPositionP)..}) | Automatic) | Null
AliquotContainer
The desired type of container that should be used to prepare and house the aliquot samples, with indices indicating grouping of samples in the same plates, if desired. This option will resolve to be the length of the SamplesIn * NumberOfReplicates.
Default Calculation: Automatically set as the PreferredContainer for the AssayVolume of the sample. For plates, attempts to fill all wells of a single plate with the same model before aliquoting into the next.
Pattern Description: An object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null or {Index, Container} or list of one or more an object of type or subtype Model[Container] or Object[Container] or a prepared sample or Automatic or Null entries or list of one or more Automatic or Null or {Index, Container} entries.
Programmatic Pattern: (((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null) | {GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | {((ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null))..} | {({GreaterEqualP[1, 1] | (Automatic | Null), (ObjectP[{Model[Container], Object[Container]}] | _String) | (Automatic | Null)} | (Automatic | Null))..}) | Automatic) | Null
AliquotPreparation
Default Calculation: Automatic resolution will occur based on manipulation volumes and container types.
ConsolidateAliquots
Protocol Options
Organizational Information
Template
A template protocol whose methodology should be reproduced in running this experiment. Option values will be inherited from the template protocol, but can be individually overridden by directly specifying values for those options to this Experiment function.
Pattern Description: An object of type or subtype Object[Protocol] or an object of type or subtype of Object[Protocol] with UnresolvedOptions, ResolvedOptions specified or Null.
Programmatic Pattern: (ObjectP[Object[Protocol]] | FieldReferenceP[Object[Protocol], {UnresolvedOptions, ResolvedOptions}]) | Null
Name
A object name which should be used to refer to the output object in lieu of an automatically generated ID number.
Post Experiment
MeasureWeight
Indicates if any solid samples that are modified in the course of the experiment should have their weights measured and updated after running the experiment. Please note that public samples are weighed regardless of the value of this option.
MeasureVolume
Indicates if any liquid samples that are modified in the course of the experiment should have their volumes measured and updated after running the experiment. Please note that public samples are volume measured regardless of the value of this option.
ImageSample
Example Calls
Basics
Ion Chromatography separates charged species from the provided sample mixtures by injection into flowing liquid that passes through the column:
Analysis Channel
Input samples can either be injected into AnionChannel or CationChannel for separation on Dionex ICS 6000:
Analysis channel can also be specified using AnionSamples and CationSamples options. To injection the first sample into AnionChannel and the rest into CationChannel, run:
Input samples can be injected into ElectrochemicalChannel for Dionex ICS 6000 with ED and UV detectors:
Gradient
Gradient profiles can also be articulated. For the anion channel, gradient is directly specified in concentration units. For example:
Buffers and gradients can also be articulated for cation channel in Percent unit for each buffer. For instance:
Buffers and gradients can also be articulated for electrochemical channel in Percent unit for each buffer. For instance:
Standards and Blanks
ExperimentIonChromatography can be used to quantify analytes in a sample, in which case, Standard samples are employed to serve as reference. A standard can be submitted before and after the injection sequence of the samples in both analysis channels simply by running:
Likewise, Blank samples can be used to see if there is any background from the injection process. To run blanks in both channels to occur between every 5 samples, use the following command:
Conductance detection
Conductivity measurement is significantly dependent on temperature. To specify the temperature of the cell where conductivity of the cation sample is measured, simply run:
Electrochemical detection
To specify a set of time-dependent voltages that will be repeated over the entire data collection period, call:
Injection Table
In addition to specifying frequencies of running standards and blanks, now the injection sequence of samples, standards, blanks or column primes/flushes can be explicitly articulated as well. In this case, an additional column flush is specified after the injection of the second sample in the anion channel:
Data Processing
Warnings and Errors
Messages (61)
BlankOptionConflict (2)
ColumnRefreshMultipleWaveformDuration (1)
ConflictingColumnRefreshSuppressorMode (1)
ConflictingElectrochemicalDetectionModes (1)
ConflictingSuppressorMode (1)
FlowRateAboveMax (1)
HPICConflictingDetectionOptions (1)
HPICGradientShortcutAmbiguity (1)
HPICGradientShortcutConflict (1)
HPICNullBlankOption (1)
HPICNullStandardOption (1)
ImbalancedAnionCationGrouping (1)
IncompatibleColumnAnalysisChannel (1)
IncompatibleColumnTechnique (1)
IncompatibleICDetector (1)
InconsistentAnalysisChannelSpecification (1)
InconsistentGradientSpecification (1)
InconsistentRefreshGradientSpecification (1)
InjectionTableForeignBlanks (3)
Return an error when the anion injection table is specified and conflicts with a specified AnionBlank:
Return an error when the cation injection table is specified and conflicts with a specified CationBlank:
Return an error when the electrochemical injection table is specified and conflicts with a specified Blank:
InjectionTableForeignSamples (3)
Return an error when the anion injection table is specified but has a different order and repetition to that of the resolved or specified anion samples:
Return an error when the cation injection table is specified but has a different order and repetition to that of the input samples:
Return an error when the electrochemical injection table is specified but has a different order and repetition to that of the input samples:
InjectionTableForeignStandards (3)
Return an error when the anion injection table is specified and conflicts with a specified AnionStandard:
Return an error when the cation injection table is specified and conflicts with a specified CationStandard:
Return an error when the electrochemical injection table is specified and conflicts with a specified Standard:
InvalidAnalysisChannelSpecification (1)
InvalidAnionGradientComposition (1)
InvalidAnionRefreshGradientComposition (1)
InvalidElectrochemicalDetectionModeOption (1)
InvalidElectrochemicalOption (1)
InvalidGradientComposition (1)
InvalidGuardColumn (1)
InvalidRefreshGradientComposition (1)
InvalidTimeSpecification (2)
MultipleElectrochemicalDetectionModes (1)
NoAnalyteInAnalysisChannel (1)
NonRecommendedICInjectionVolume (1)
ObjectDoesNotExist (5)
Throw a message if we have a sample that does not exist (name form):
Throw a message if we have a container that does not exist (name form):
Throw a message if we have a sample that does not exist (ID form):
Throw a message if we have a container that does not exist (ID form):
Return a warning when any specific object does not exist in the database:
OverwritingGradient (1)
OverwritingSamplelessChannelOptions (1)
pHCalibrationOptionConflict (1)
pHCalibrationTarget (1)
SampleOptionConflict (1)
Possible Issues
Column flush
The column will end up being stored in whatever the final gradient solvent conditions are so that should be considered for column protection.
Column fouling
Consider using a GuardColumn to preserve column lifetime. Examine injections of standards over time to ensure that the column is performing to satisfaction.
Filter sample and buffer
Last modified on Wed 8 Oct 2025 13:12:51