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Object[Data, CapillaryIsoelectricFocusing]

Analytical data collected by a capillary IsoElectric Focusing (cIEF) experiment on samples for electrophoretic separation over a linear pH gradient.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, CapillaryIsoelectricFocusing, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    Standards

    Standard data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data]

    Blanks

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data]

General

Experimental Results

    SeparationData

    The electropherogram time lapse of UV Absorbance (A280) vs. position in the capillary over 32 time points in fucosing.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 Pixels', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Pixel, AbsorbanceUnit}]

    UVAbsorbanceData

    The electropherogram of UV Absorbance (A280) vs. position in the capillary.
    Format: Single
    Class: QuantityArray
    Unit: ['1 Pixels', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Pixel, AbsorbanceUnit}]

    ProcessedUVAbsorbanceData

    The electropherogram of UV Absorbance (A280) vs. position in the capillary as processed by the Compass for iCE software.
    Format: Single
    Class: QuantityArray
    Unit: ['1 Pixels', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Pixel, AbsorbanceUnit}]

    UVAbsorbanceBackgroundData

    The electropherogram of UV Absorbance background vs. position in the capillary.
    Format: Single
    Class: QuantityArray
    Unit: ['1 Pixels', '1 AbsorbanceUnit']
    Programmatic Pattern: QuantityCoordinatesP[{Pixel, AbsorbanceUnit}]

    FluorescenceData

    The electropherogram of native fluorescence vs. position in the capillary.
    Format: Multiple

    [[1]] Exposure Time

      Header: Exposure Time
      Class: Real
      Unit: 1 second
      Programmatic Pattern: GreaterP[0 Second]

    [[2]] Native Fluorescence

      Header: Native Fluorescence
      Class: QuantityArray
      Unit: ['1 Pixels', '1 Rfus']
      Programmatic Pattern: QuantityCoordinatesP[{Pixel, RFU}]

    FluorescenceBackgroundData

    The electropherogram of native fluorescence background vs. position in the capillary.
    Format: Multiple

    [[1]] Exposure Time

      Header: Exposure Time
      Class: Real
      Unit: 1 second
      Programmatic Pattern: GreaterP[0 Second]

    [[2]] Native Fluorescence

      Header: Native Fluorescence
      Class: QuantityArray
      Unit: ['1 Pixels', '1 Rfus']
      Programmatic Pattern: QuantityCoordinatesP[{Pixel, RFU}]

    ProcessedFluorescenceData

    The electropherogram of native fluorescence background vs. position in the capillary as processed by the Compass for iCE software.
    Format: Multiple

    [[1]] Exposure Time

      Header: Exposure Time
      Class: Real
      Unit: 1 second
      Programmatic Pattern: GreaterP[0 Second]

    [[2]] Native Fluorescence

      Header: Native Fluorescence
      Class: QuantityArray
      Unit: ['1 Pixels', '1 Rfus']
      Programmatic Pattern: QuantityCoordinatesP[{Pixel, RFU}]

    CurrentData

    The Current vs. time curve of focusing.
    Format: Single
    Class: QuantityArray
    Unit: ['1 second', '1 milliampere']
    Programmatic Pattern: QuantityCoordinatesP[{Second, Milliampere}]

    VoltageData

    The Voltage vs. time curve of focusing.
    Format: Single
    Class: QuantityArray
    Unit: ['1 second', '1 volt']
    Programmatic Pattern: QuantityCoordinatesP[{Second, Volt}]

Data Processing

    IsoelectricPointData

    The electropherogram of pH vs. distance based on internal standards.
    Format: Single
    Class: QuantityArray
    Unit: ['1 Pixels', 'None']
    Programmatic Pattern: QuantityCoordinatesP[{Pixel, None}]

Analysis & Reports

    SmoothingAnalyses

    Smoothing analysis performed on isoelectric focusing data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    PeaksAnalyses

    Peak picking analyses performed on isoelectric focusing data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    FitAnalyses

    Fit analyses performed on isoelectric focusing data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
Last modified on Mon 26 Sep 2022 15:43:44