ecl-logo Documentation

Object[Data, DigitalPCR]

Digital Polymerase Chain Reaction randomly distributes targets by partitioning sample into nanoliter-sized droplets before amplifying them and counts the number of droplets that have fluorescence signal out of the total.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DigitalPCR, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DigitalPCR]
    Pattern Description: Object[Data, DigitalPCR]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Data Processing

    SummaryDataFile

    The file containing the summary data table generated by the instrument software during a DigitalPCR run.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

Experimental Results

    DropletExcitationWavelengths

    For each member of DropletEmissionWavelengths, the wavelengths of the excitation filters used to illuminate the droplets.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Meter]

    DropletEmissionWavelengths

    The wavelengths of the emission filters used to collect fluorescence signals from the droplets in relation to indexed fields in DropletAmplitudes.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Meter]

    DropletAmplitudes

    Raw data representing the magnitude of fluorescence signal measured in each detection channel from each droplet in the sample well.
    Format: Single

    [[1]] 517 nm

      Header: 517 nm
      Class: QuantityArray
      Unit: 1 Rfus
      Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    [[2]] 556 nm

      Header: 556 nm
      Class: QuantityArray
      Unit: 1 Rfus
      Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    [[3]] 665 nm

      Header: 665 nm
      Class: QuantityArray
      Unit: 1 Rfus
      Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    [[4]] 694 nm

      Header: 694 nm
      Class: QuantityArray
      Unit: 1 Rfus
      Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    DropletAmplitudes517nm

    Array of fluorescence signal magnitudes measured from each droplet in 517 nm emission channel.
    Format: Single
    Class: QuantityArray
    Unit: 1 Rfus
    Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    DropletAmplitudes556nm

    Array of fluorescence signal magnitudes measured from each droplet in 556 nm emission channel.
    Format: Single
    Class: QuantityArray
    Unit: 1 Rfus
    Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    DropletAmplitudes665nm

    Array of fluorescence signal magnitudes measured from each droplet in 665 nm emission channel.
    Format: Single
    Class: QuantityArray
    Unit: 1 Rfus
    Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

    DropletAmplitudes694nm

    Array of fluorescence signal magnitudes measured from each droplet in 694 nm emission channel.
    Format: Single
    Class: QuantityArray
    Unit: 1 Rfus
    Programmatic Pattern: _?(QuantityVectorQ[#1, RFU] & )

Analysis & Reports

    AverageDropletAmplitudes

    For each member of EmissionWavelengths, the mean magnitude of fluorescence signal from a population of droplets.
    Format: Multiple

    [[1]] Signal Positive

      Header: Signal Positive
      Class: Real
      Unit: 1 Rfus
      Programmatic Pattern: GreaterEqualP[0 RFU]

    [[2]] Signal Negative

      Header: Signal Negative
      Class: Real
      Unit: 1 Rfus
      Programmatic Pattern: GreaterEqualP[0 RFU]

    DropletSignalThresholds

    For each member of EmissionWavelengths, the amplitude cut-off value used to separate the population of droplets with positive signal from droplets with no signal for a target.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: GreaterEqualP[0 RFU]

    DropletCounts

    For each member of EmissionWavelengths, the number of droplets with positive and negative signals.
    Format: Multiple

    [[1]] Signal Positive

      Header: Signal Positive
      Class: Integer
      Programmatic Pattern: GreaterEqualP[0, 1]

    [[2]] Signal Negative

      Header: Signal Negative
      Class: Integer
      Programmatic Pattern: GreaterEqualP[0, 1]

    ReferenceAverageDroplet Amplitudes

    For each member of ReferenceEmissionWavelengths, the mean magnitude of fluorescence signal from a population of droplets.
    Format: Multiple

    [[1]] Signal Positive

      Header: Signal Positive
      Class: Real
      Unit: 1 Rfus
      Programmatic Pattern: GreaterEqualP[0 RFU]

    [[2]] Signal Negative

      Header: Signal Negative
      Class: Real
      Unit: 1 Rfus
      Programmatic Pattern: GreaterEqualP[0 RFU]

    ReferenceDropletSignal Thresholds

    For each member of ReferenceEmissionWavelengths, the amplitude cut-off value used to separate the population of droplets with positive signal from droplets with no signal for a target.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: GreaterEqualP[0 RFU]

    ReferenceDropletCounts

    For each member of ReferenceEmissionWavelengths, the number of droplets with positive and negative signals.
    Format: Multiple

    [[1]] Signal Positive

      Header: Signal Positive
      Class: Integer
      Programmatic Pattern: GreaterEqualP[0, 1]

    [[2]] Signal Negative

      Header: Signal Negative
      Class: Integer
      Programmatic Pattern: GreaterEqualP[0, 1]
Last modified on Mon 26 Sep 2022 15:43:44
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.