ecl-logo Documentation

Object[Data, DynamicLightScattering]

Data from a dynamic light scattering experiment.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DynamicLightScattering, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DynamicLightScattering]
    Pattern Description: Object[Data, DynamicLightScattering]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Sample Dilution

    AssayProtein

    The Model(Molecule,Protein) member of the Composition field whose dilution concentrations are tracked in the DilutionConcentration, which are used to calculate the B22 and kD, or G22 values.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Model[Molecule]

    AssayProteinMass Concentrations

    The concentration of the AssayProtein before any dilutions are performed.
    Format: Single
    Class: Real
    Unit: 1 milligram per milliliter
    Programmatic Pattern: Milligram Hold[GreaterEqualP[0 ----------]] Milliliter

    ReplicateDilutionCurve

    Indicates if a NumberOfReplicateConcentrations number of DilutionCurves or SerialDilutionCurves are made for each input sample. When ReplicateDilutionCurve is False, the replicate DLS measurements for the B22kD or G22 assay are made from aliquots of a given concentration of the DilutionCurve or SerialDilutionCurve. When ReplicateDilutionCurve is True, the replicate DLS measurements for B22kD or G22 assay are made from the same concentration of each of the StandardCurves or SerialDilutionCurves created from a given input sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    DilutionFactors

    The ratios of the volume of input sample to the sum of the input sample volume and diluent volume for each dilution.
    Format: Single
    Class: Expression
    Programmatic Pattern: {(_Integer | _Real | _Rational)..} | {Null..} | Null

    DilutionConcentrations

    For each member of SamplesIn, the protein mass concentrations of each dilution.
    Format: Single
    Class: Expression
    Programmatic Pattern: Milligram Hold[{GreaterEqualP[0 ----------]..} | {Null..} | Null] Milliliter

    Diluent

    For each member of SamplesIn, the sample that is used to dilute the sample to a concentration series.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample] | Model[Sample]

    DilutionMixVolume

    The volume that is pipetted out and in of the dilution to mix the sample with the Diluent to make the DilutionCurve.
    Format: Single
    Class: Real
    Unit: 1 microliter
    Programmatic Pattern: GreaterEqualP[0 Microliter]

    DilutionNumberOfMixes

    The number of pipette out and in cycles that is used to mix the sample with the Diluent to make the DilutionCurve.
    Format: Single
    Class: Integer
    Programmatic Pattern: RangeP[0, 20, 1]

    DilutionMixRate

    The speed at which the DilutionMixVolume is pipetted out and in of the dilution to mix the sample with the Diluent to make the DilutionCurve.
    Format: Single
    Class: Real
    Unit: 1 microliter per second
    Programmatic Pattern: Microliter Microliter Hold[RangeP[0.4 ----------, 250 ----------]] Second Second

Sample Loading

    BlankBuffer

    The 0 mg/mL buffer-only blank sample that is required for B22kD and G22 assays.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Sample] | Model[Sample]

    AssayContainer

    The capillary strip that the sample is assayed in.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Object[Container, Plate]]

    AssayPosition

    The well position of the sample in the AssayContainer.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    AssayContainers

    The capillary strips that each sample dilution is assayed in.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Object[Container, Plate]]

    AssayPositions

    For each member of AssayContainers, the well positions of the sample dilutions.
    Format: Multiple
    Class: String
    Programmatic Pattern: _String

Isothermal Stability

    MeasurementDelayTime

    The length of time between the consecutive DLS measurements for a specific AssayContainer well during an IsothermalStability assay.
    Format: Single
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    IsothermalMeasurements

    The number of DLS measurements that are made during the IsothermalStability assay.
    Format: Single
    Class: Integer
    Programmatic Pattern: RangeP[1, 20, 1]

    IsothermalRunTime

    The length of the IsothermalStability assay during which DLS measurements are being made every MeasurementDelayTime.
    Format: Single
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Second]

    IsothermalAttenuator Adjustment

    Indicates if the attenuator level is automatically set for each DLS measurement throughout the IsothermalStability assay. If False, the attenuator level is automatically set for the first DLS measurement and the same level is used throughout the assay.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

Experimental Results

    ZAverageDiameter

    The average hydrodynamic diameter of particles in solution.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterEqualP[0 Nanometer]

    ZAverageDiffusionCoefficient

    Diffusion coefficeint calculated from the CorrelationCurve which is inversely proportional to particle size.
    Format: Single
    Class: Real
    Unit: 1 meter squared per second
    Programmatic Pattern: Meter Hold[GreaterEqualP[0 (Meter ------)]] Second

    DiameterStandardDeviation

    The standard deviation of the ZAverageDiameter.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterEqualP[0 Nanometer]

    PolydispersityIndex

    A measure of the heterogeneity of the calculated hydrodynamic diameters of particles in solution.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterEqualP[0]

    ScatteredLightIntensity

    The amount of light that reaches the scattered light detector in counts per second.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterEqualP[0]

    CorrelationCurve

    A series of data points which describes the correlation between scattered light intensity over time for the sample. This correlation typically decays from 1 to 0 over time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 microsecond', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Micro Second, ArbitraryUnit}]

    CorrelationCurveFitVariance

    A measure of the fit of multiple exponential decay function to the measured CorrelationCurve.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterEqualP[0]

    IntensityDistribution

    Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution. Since larger particles scatter more light, larger particles have a higher scattered light intensity per particle in the IntensityDistribution.
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]

    MassDistribution

    Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution. In the MassDistribution, as opposed to the IntensityDistribution, the scattered light intensity at each hydrodynamic diameter is corrected for particle size, so this trace gives a more accurate representation of the number of particles of each size in solution.
    Format: Single
    Class: QuantityArray
    Unit: ['1 nanometer', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]

    ZAverageDiameters

    For each time point or MassConcentration, the average hydrodynamic diameter of particles in solution.
    Format: Multiple

    [[1]] Time or Mass Concentration

      Header: Time or Mass Concentration
      Class: VariableUnit

    [[2]] Z-Average Diameter

      Header: Z-Average Diameter
      Class: Real
      Unit: 1 nanometer

    PolydispersityIndicies

    For each time point or MassConcentration, a measure of the heterogeneity of the calculated hydrodynamic diameters of particles in solution.
    Format: Multiple

    [[1]] Time or Mass Concentration

      Header: Time or Mass Concentration
      Class: VariableUnit

    [[2]] Polydispersity Index

      Header: Polydispersity Index
      Class: Real

    ScatteredLightIntensities

    For each time point or MassConcentration, the amount of light that reaches the scattered light detector in counts per second, .
    Format: Multiple

    [[1]] Time or Mass Concentration

      Header: Time or Mass Concentration
      Class: VariableUnit

    [[2]] Scattered Light Intensity

      Header: Scattered Light Intensity
      Class: Real

    CorrelationCurves

    For each time point or MassConcentration, a series of data points which describes the correlation between scattered light intensity over time for the sample. This correlation typically decays from 1 to 0 over time.
    Format: Multiple

    [[1]] Time or Mass Concentration

      Header: Time or Mass Concentration
      Class: VariableUnit

    [[2]] Correlation Curve

      Header: Correlation Curve
      Class: QuantityArray
      Unit: ['1 microsecond', '1 ArbitraryUnits']

    CorrelationCurveFitVariances

    For each time point or MassConcentration, a measure of the fit of multiple exponential decay function to the measured CorrelationCurve.
    Format: Multiple

    [[1]] Time or Mass Concentration

      Header: Time or Mass Concentration
      Class: VariableUnit

    [[2]] Correlation Curve Fit Variance

      Header: Correlation Curve Fit Variance
      Class: Real

    KirkwoodBuffIntegral

    Also known as G22 - a measure of the colloidal stability of a solution at higher concentrations. Positive G22 values indicate weak net-attractive intermolecular forces between particles, while negative G22 values inidicate net-repulsive interactions.
    Format: Multiple

    [[1]] Mass Concentration

      Header: Mass Concentration
      Class: Real
      Unit: 1 milligram per milliliter

    [[2]] Kirkwood-Buff Integral

      Header: Kirkwood-Buff Integral
      Class: Real
      Unit: 1 milliliter per gram

    R90OverK

    R90 over K is the ratio between the Rayleigh Ratio (R90) and an optical constant (K) - this value is proportional to the Kirkwood-Buff Integral (G22).
    Format: Multiple

    [[1]] Mass Concentration

      Header: Mass Concentration
      Class: Real
      Unit: 1 milligram per milliliter

    [[2]] R90/K

      Header: R90/K
      Class: Real
      Unit: 1 gram squared per milliliter mole

    DiffusionInteractionParameter Statistics

    The diffusion interaction parameter (kD) is a measure of colloidal stability at lower protein concentrations (<10 mg/mL). A positive kD value indicates weak net-repulsive intermolecular forces between particles, while a negative kD value indicates net-attractive interactions.
    Format: Single

    [[1]] Diffusion Interaction Parameter (kD)

      Header: Diffusion Interaction Parameter (kD)
      Class: Real
      Unit: 1 milliliter per gram

    [[2]] kD Slope

      Header: kD Slope
      Class: Real

    [[3]] kD Intercept

      Header: kD Intercept
      Class: Real

    [[4]] Calculated Hydrodynamic Diameter

      Header: Calculated Hydrodynamic Diameter
      Class: Real
      Unit: 1 nanometer

    [[5]] R-Squared

      Header: R-Squared
      Class: Real

    [[6]] kD Fit Quality

      Header: kD Fit Quality
      Class: Real

    DiffusionInteractionParameter Data

    Data from which the diffusion interaction parameter is calculated.
    Format: Single
    Class: QuantityArray
    Unit: ['1 milligram per milliliter', '1 meter squared per second']
    Programmatic Pattern: Milligram Meter Meter Hold[QuantityCoordinatesP[{----------, -----------}]] Milliliter Second

    SecondVirialCoefficient Statistics

    The second virial coefficient (B22) is a measure of colloidal stability at lower protein concentrations (<10 mg/mL). A positive B22 value indicates weak net-repulsive intermolecular forces between particles, while a negative B22 value inidicates net-attractive interactions.
    Format: Single

    [[1]] Second Virial Coefficient (B22)

      Header: Second Virial Coefficient (B22)
      Class: Real
      Unit: 1 milliliter mole per gram squared

    [[2]] B22 Slope

      Header: B22 Slope
      Class: Real

    [[3]] B22 Intercept

      Header: B22 Intercept
      Class: Real

    [[4]] Calculated Molecular Weight

      Header: Calculated Molecular Weight
      Class: Real
      Unit: 1 kilogram per mole

    [[5]] R-Squared

      Header: R-Squared
      Class: Real

    [[6]] B22 Fit Quality

      Header: B22 Fit Quality
      Class: Real

    SecondVirialCoefficientData

    Data from which the second virial coefficient is calculated.
    Format: Single
    Class: QuantityArray
    Unit: ['1 milligram per milliliter', '1 mole per gram']
    Programmatic Pattern: Milligram Mole Hold[QuantityCoordinatesP[{----------, ----}]] Milliliter Gram

Data Processing

    RawDataFiles

    The file(s) containing the raw unprocessed data generated by the instrument.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

Analysis & Reports

    MassDistributionAnalyses

    Peak picking analyses performed on the MassDistribution data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    IntensityDistributionAnalyses

    Peak picking analyses performed on the IntensityDistribution data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    DynamicLightScattering Analyses

    Analysis of dynamic light scattering to determine the particle size, diffusion interaction parameter, second virial coefficient.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:44
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.