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Object[Data, FlowCytometry]

Data collected from flow cytometry experiments, a laser-based technology used to count cells, sort cells, detect biomarkers and/or detect protein modifications.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FlowCytometry, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FlowCytometry]
    Pattern Description: Object[Data, FlowCytometry]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Method Information

    TriggerDetector

    The detector used to determine what signals count as an event.
    Format: Single
    Class: Expression
    Programmatic Pattern: FlowCytometryDetectorP

    TriggerThreshold

    The level of the intensity detected by TriggerDetector must fall above to be classified as an event.
    Format: Single
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: RangeP[0.01 Percent, 100 Percent]

    SecondaryTriggerDetector

    The additional detector used to determine what signals count as an event.
    Format: Single
    Class: Expression
    Programmatic Pattern: FlowCytometryDetectorP

    SecondaryTriggerThreshold

    The level of the intensity detected by SecondaryTriggerDetector must fall above to be classified as an event.
    Format: Single
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: RangeP[0.01 Percent, 100 Percent]

    Detectors

    The detectors used to detect light scattered off the samples.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: FlowCytometryDetectorP

    DetectionLabels

    For each member of Detectors, the tag, including fluorescent or non-fluorescent chemical compounds or proteins, attached to the sample that will be analyzed by the Detectors.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Model[Molecule]]

    ExcitationWavelengths

    The wavelength(s) that were used to excite fluorescence and scatter off the samples.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: FlowCytometryExcitationWavelengthP

    ExcitationPowers

    For each member of ExcitationWavelengths, the power was used to excite fluorescence and scatter off the samples.
    Format: Multiple
    Class: Real
    Unit: 1 milliwatt
    Programmatic Pattern: RangeP[0 Milli Watt, 100 Milli Watt]

    Gains

    For each member of Detectors, the voltage the PMT was set to to detect the scattered light off the sample.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: GreaterEqualP[0 Volt]

Data Processing

    RunListFile

    The flow cytometry rlst file which stores the metadata about how the experiment was performed.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

Experimental Results

    Volume

    The volume of sample analysed by the flow cytometer during the run.
    Format: Single
    Class: Real
    Unit: 1 milliliter
    Programmatic Pattern: GreaterP[0 Milliliter]

    Events

    The total number of events detected by the flow cytometer during the run.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterP[0]

    CellCount

    The number of events detected per volume.
    Format: Single
    Class: Real
    Unit: 1 Events per milliliter
    Programmatic Pattern: Event Hold[GreaterP[0 ----------]] Milliliter

    EventTimes

    The times in which each event occurred after data collection started.
    Format: Multiple
    Class: Real
    Unit: 1 second
    Programmatic Pattern: GreaterP[0 Millisecond]

Analysis & Reports

    FlowCytometryAnalyses

    A list of flow cytometry analyses performed on this data object.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:44
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