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Object[Data, FluorescencePolarization]

Measurements of intensity of a fluorescence emission at a fixed wavelength.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescencePolarization, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescencePolarization]
    Pattern Description: Object[Data, FluorescencePolarization]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Fluorescence Measurement

    ReadLocation

    The side of the microplate from which the readings were taken.
    Format: Single
    Class: Expression
    Programmatic Pattern: ReadLocationP

Experimental Results

    ParallelIntensities

    For each member of DualEmissionWavelengths, the measurements of fluorescence intensity oriented parallel to the horizontally polarized excitation light.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: UnitsP[RFU]

    PerpendicularIntensities

    For each member of DualEmissionWavelengths, the measurements of fluorescence intensity oriented perpendicular to the horizontally polarized excitation light.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: UnitsP[RFU]

    Anisotropy

    For each member of DualEmissionWavelengths, the fluorescence anisotropy calculated as the difference between parallel and perpendicular emission intensities normalized by the total fluorescence intensity of the emission light (in three dimensions).
    Format: Multiple
    Class: Real
    Unit: 1 milli AnisotropyUnits
    Programmatic Pattern: UnitsP[AnisotropyUnit]

    Polarization

    For each member of DualEmissionWavelengths, the fluorescence polarization calculated as the difference between parallel and perpendicular emission intensities normalized by the total fluorescence intensity of the emission light (in two dimensions).
    Format: Multiple
    Class: Real
    Unit: 1 milli PolarizationUnits
    Programmatic Pattern: UnitsP[PolarizationUnit]

    Temperature

    Temperature inside the sample chamber at the time of acquisition.
    Format: Single
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Kelvin]

    DetectorRangeExceeded

    Indicates if any of the recorded intensities maxed out the instrument's detector.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    InvalidValuesExcluded

    Indicates if any of the recorded intensities are below 0 RFU and have been deleted from the final result.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP
Last modified on Mon 26 Sep 2022 15:43:44
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