ecl-logo Documentation

Object[Data, FluorescencePolarizationKinetics]

Fluorescence measurements of a sample monitored over a period of time to study reaction trajectories.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescencePolarizationKinetics, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescencePolarizationKinetics]
    Pattern Description: Object[Data, FluorescencePolarizationKinetics]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Fluorescence Measurement

    ReadLocation

    The side of the microplate from which the readings were taken.
    Format: Single
    Class: Expression
    Programmatic Pattern: ReadLocationP

Analysis & Reports

    RateFittingAnalyses

    The data objects used to create a standard curve relating fluorescence to concentration for this object.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

Experimental Results

    ParallelTrajectories

    Emission fluorescence readings (parallel to the excitation plane) taken as a function of time, for the configuration of excitation wavelength, emission wavelength and sensitivity.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Second, RFU}]

    PerpendicularTrajectories

    Emission fluorescence readings (perpendicular to the excitation plane) taken as a function of time, for the configuration of excitation wavelength, secondary emission wavelength and secondary sensitivity.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Second, RFU}]

    Anisotropy

    For each member of DualEmissionWavelengths, the fluorescence anisotropy calculated as the difference between parallel and perpendicular emission intensities normalized by the total fluorescence intensity of the emission light (in three dimensions).
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 milli AnisotropyUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Second, AnisotropyUnit}]

    Polarization

    For each member of DualEmissionWavelengths, the fluorescence polarization calculated as the difference between parallel and perpendicular emission intensities normalized by the total fluorescence intensity of the emission light (in two dimensions).
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 milli PolarizationUnits']
    Programmatic Pattern: QuantityCoordinatesP[{Second, PolarizationUnit}]

    Temperature

    Temperature readings taken in the sample chamber, for the trajectories recorded.
    Format: Single
    Class: QuantityArray
    Unit: ['1 second', '1 degree Celsius']
    Programmatic Pattern: QuantityCoordinatesP[{Second, Celsius}]

    DetectorRangeExceeded

    Indicates if any of the recorded intensities maxed out the instrument's detector.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    InvalidValuesExcluded

    Indicates if any of the recorded intensities are below 0 RFU and have been deleted from the final result.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP
Last modified on Mon 26 Sep 2022 15:43:44
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.