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Object[Data, ImageCells]

Image data captured by a microscope.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, ImageCells, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, ImageCells]
    Pattern Description: Object[Data, ImageCells]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

    Software

    The acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    SoftwareVersion

    Indicates the version of the acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    DateAcquired

    The date on which the image was acquired.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

General

Experimental Results

    TimeSeriesData

    For each member of Timepoints, the data object containing images acquired from the sample.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, ImageCells]

    ZSeriesData

    For each member of ZPlanes, the data object containing images acquired from the sample at a specific plane along the z-axis.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, ImageCells]

    ImagingSiteData

    The list of data objects containing images acquired from the sample at a specific imaging site in the XY plane.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, ImageCells]

    ImageFiles

    For each member of ImagingChannels, the raw image acquired from the sample.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

Imaging

    Objective

    The objective lens used to acquire the image from SamplesIn.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Part, Objective]

    ObjectiveMagnification

    The magnification power used to acquire the image which is defined by the ratio between the dimensions of the image and the sample.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterEqualP[1]

    ObjectiveNumericalAperture

    The numerical aperture of the objective used to acquire the image.
    Format: Single
    Class: Real
    Programmatic Pattern: GreaterP[0]

    ImagingChannels

    Indicates the fluorescence imaging channels used to acquire the images. Each imaging channel has a unique combination of excitation and emission wavelengths.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: MicroscopeImagingChannelP

    ExposureTimes

    For each member of ImagingChannels, the length of time that the camera collects the signal from the sample.
    Format: Multiple
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    ExcitationWavelengths

    For each member of ImagingChannels, the wavelength of excitation light used to illuminate the sample when imaging with ConfocalFluorescence or Epifluorescence Mode.
    Format: Multiple
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    ExcitationPowers

    For each member of ImagingChannels, the percent of maximum intensity of the light source used to illuminate the sample.
    Format: Multiple
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: GreaterP[0 Percent]

    TransmittedLightPowers

    For each member of ImagingChannels, the percent of maximum intensity of the transmitted light used to illuminate the sample.
    Format: Multiple
    Class: Real
    Unit: 1 percent
    Programmatic Pattern: GreaterP[0 Percent]

    DichroicFilters

    For each member of ImagingChannels, the dichroic filter used in the light path at the time the image was acquired to selectively pass fluorescence emitted from the sample and reflect excitation light.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Part, OpticalFilter]

    EmissionFilters

    For each member of ImagingChannels, the optical filter used in the light path at the time the image was acquired to selectively pass fluorescence emitted from the sample.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Part, OpticalFilter]

    ImageCorrections

    For each member of ImagingChannels, the correction method applied to the image after acquisition to remove stray light or mitigate the uneven illumination.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: MicroscopeImageCorrectionP

Analysis & Reports

    MicroscopeOverlay

    Microscope image analyses that used this data in overlaying multiple images while varying transparency, color, contrast and brightness.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    CellCountAnalyses

    A list of the cell counting analyses that were performed on this microscope data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:44
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