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Object[Data, Microscope]

Image data captured by a microscope.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Microscope, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Microscope]
    Pattern Description: Object[Data, Microscope]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

    Software

    The acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    SoftwareVersion

    Indicates the version of the acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

General

Experimental Results

    Images

    The images acquired from the sample by a microscope.
    Format: Multiple

    [ImageFile]

    [ProtocolBatchNumber]

      Header: ProtocolBatchNumber
      Class: Integer

    [DateAcquired]

      Header: DateAcquired
      Class: Date

    [Mode]

      Header: Mode
      Class: Expression

    [ObjectiveMagnification]

      Header: ObjectiveMagnification
      Class: Real

    [ObjectiveNumericalAperture]

      Header: ObjectiveNumericalAperture
      Class: Real

    [Objective]

    [ImageTimepoint]

      Header: ImageTimepoint
      Class: Integer

    [ImageZStep]

      Header: ImageZStep
      Class: Integer

    [ImagingSite]

      Header: ImagingSite
      Class: Integer

    [ExcitationWavelength]

      Header: ExcitationWavelength
      Class: Real
      Unit: 1 nanometer

    [EmissionWavelength]

      Header: EmissionWavelength
      Class: Real
      Unit: 1 nanometer

    [DichroicFilterWavelength]

      Header: DichroicFilterWavelength
      Class: Real
      Unit: 1 nanometer

    [EmissionFilter]

    [DichroicFilter]

    [ExcitationPower]

      Header: ExcitationPower
      Class: Real
      Unit: 1 percent

    [TransmittedLightPower]

      Header: TransmittedLightPower
      Class: Real
      Unit: 1 percent

    [ExposureTime]

      Header: ExposureTime
      Class: Real
      Unit: 1 millisecond

    [FocalHeight]

      Header: FocalHeight
      Class: Real
      Unit: 1 micrometer

    [ImageSizeX]

      Header: ImageSizeX
      Class: Real
      Unit: 1 Pixels

    [ImageSizeY]

      Header: ImageSizeY
      Class: Real
      Unit: 1 Pixels

    [ImageScaleX]

      Header: ImageScaleX
      Class: Real
      Unit: 1 micrometer per Pixels

    [ImageScaleY]

      Header: ImageScaleY
      Class: Real
      Unit: 1 micrometer per Pixels

    [ImageBitDepth]

      Header: ImageBitDepth
      Class: Integer

    [PixelBinning]

      Header: PixelBinning
      Class: Integer

    [ImageCorrection]

      Header: ImageCorrection
      Class: Expression

    [StagePositionX]

      Header: StagePositionX
      Class: Real
      Unit: 1 micrometer

    [StagePositionY]

      Header: StagePositionY
      Class: Real
      Unit: 1 micrometer

    [ImagingSiteRow]

      Header: ImagingSiteRow
      Class: Integer

    [ImagingSiteColumn]

      Header: ImagingSiteColumn
      Class: Integer

    [ImagingSiteRowSpacing]

      Header: ImagingSiteRowSpacing
      Class: Real
      Unit: 1 micrometer

    [ImagingSiteColumnSpacing]

      Header: ImagingSiteColumnSpacing
      Class: Real
      Unit: 1 micrometer

    [WellCenterOffsetX]

      Header: WellCenterOffsetX
      Class: Real
      Unit: 1 micrometer

    [WellCenterOffsetY]

      Header: WellCenterOffsetY
      Class: Real
      Unit: 1 micrometer

    PhaseContrastImage

    Returns the image taken under phase contrast conditions.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[PhaseContrastImageFile]}, ImportCloudFile[Field[PhaseContrastImageFile]]]

    FluorescenceImage

    Returns the primary fluorescence image.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[FluorescenceImageFile]}, ImportCloudFile[Field[FluorescenceImageFile]]]

    SecondaryFluorescenceImage

    Returns the secondary fluorescence image.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[SecondaryFluorescenceImageFile]}, ImportCloudFile[Field[SecondaryFluorescenceImageFile]]]

    TertiaryFluorescenceImage

    Returns the tertiary fluorescence image.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[TertiaryFluorescenceImageFile]}, ImportCloudFile[Field[TertiaryFluorescenceImageFile]]]

    PhaseContrastImageFile

    The raw image taken under phase contrast conditions.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[EmeraldCloudFile]]
    Relation: Object[EmeraldCloudFile]

    FluorescenceImageFile

    The raw fluorescence image captured at the primary emission wavelength.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[EmeraldCloudFile]]
    Relation: Object[EmeraldCloudFile]

    SecondaryFluorescenceImage File

    The raw fluorescence image captured at the secondary emission wavelength.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[EmeraldCloudFile]]
    Relation: Object[EmeraldCloudFile]

    TertiaryFluorescenceImageFile

    The raw fluorescence image captured at the tertiary emission wavelength.
    Format: Single
    Class: Link
    Programmatic Pattern: ObjectP[Object[EmeraldCloudFile]]
    Relation: Object[EmeraldCloudFile]

Analysis & Reports

    MicroscopeOverlay

    Microscope image analyses that used this data in overlaying multiple images while varying transparency, color, contrast and brightness.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    AdjustmentAnalyses

    Microscope image analyses that used this data in adjusting contrast and brightness of image channels.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    CellCountAnalyses

    A list of the cell counting analyses that were performed on this microscope data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    PhaseContrastCellCount

    The most recent cell count of the phase contrast image analysis.
    Format: Computable
    Programmatic Pattern: GreaterEqualP[0]
    Expression: SafeEvaluate[{Field[CellCountAnalyses]}, Computables`Private`cellCountComputable[Field[CellCountAnalyses], PhaseContrastImage]]

    FluorescenceCellCount

    The most recent cell count of the image analysis on the primary fluorescence image.
    Format: Computable
    Programmatic Pattern: GreaterEqualP[0]
    Expression: SafeEvaluate[{Field[CellCountAnalyses]}, Computables`Private`cellCountComputable[Field[CellCountAnalyses], FluorescenceImage]]

    SecondaryFluorescenceCell Count

    The most recent cell count of the image analysis on the secondary fluorescence image.
    Format: Computable
    Programmatic Pattern: GreaterEqualP[0]
    Expression: SafeEvaluate[{Field[CellCountAnalyses]}, Computables`Private`cellCountComputable[Field[CellCountAnalyses], SecondaryFluorescenceImage]]

    TertiaryFluorescenceCellCount

    The most recent cell count of the image analysis on the tertiary fluorescence image.
    Format: Computable
    Programmatic Pattern: GreaterEqualP[0]
    Expression: SafeEvaluate[{Field[CellCountAnalyses]}, Computables`Private`cellCountComputable[Field[CellCountAnalyses], TertiaryFluorescenceImage]]
Last modified on Mon 26 Sep 2022 15:43:44
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