Object[Protocol, FPLC]
Fast protein liquid chromatography (FPLC) experiment that is used to separate and analyze mixtures of proteins.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, FPLC, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, FPLC]
Pattern Description: Object[Protocol, FPLC]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLogs
The instrumentation trace files that monitored and recorded the execution of this robotic liquid handling.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The instrument containing a pump, column oven, flow cell detector, and fraction collector used to execute this experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Scale
Indicates whether the run is meant to preparatively purify the samples or meant to simply analyze the purity of the samples.
Format: Single
Class: Expression
Programmatic Pattern: PurificationScaleP
SeparationMode
The type of chromatographic separation describing the mobile and stationary phase interplay.
Format: Single
Class: Expression
Programmatic Pattern: SeparationModeP
Detectors
Indicates the types of measurements performed for the experiment and available on the Instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: ChromatographyDetectorTypeP
InjectionType
Whether the system introduces sample in to the flow path via the automated injection module (Autosampler) or directly from an inlet line submerged into the samples (FlowInjection).
Format: Single
Class: Expression
Programmatic Pattern: FPLCInjectionTypeP
SampleLoop
When InjectionType -> Superloop, the tubing used to hold the sample before introduction into the system.
Format: Single
Class: Link
Programmatic Pattern: _Link
Mixer
The fluid chamber designed to agitate liquid. A joining column is used when no mixer is requested.
Format: Single
Class: Link
Programmatic Pattern: _Link
FlowCell
When a non-default fluid chamber for Absorbance measurement is requested, the part swapped in for the default one.
Format: Single
Class: Link
Programmatic Pattern: _Link
Columns
The column(s) used to separate the input mixture.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
MaxColumnPressure
The maximum allowable pressure across all of the Columns.
Format: Single
Class: Real
Unit: 1 megapascal
Programmatic Pattern: GreaterEqualP[0 Megapascal]
ColumnJoins
The connections used to link multiple Columns.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferACaps
For each member of BufferASelection, the cap used to aspirate during this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferBCaps
For each member of BufferBSelection, the cap used to aspirate during this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SampleCaps
When InjectionMode -> FlowInjection, the caps that aspirate each sample container.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
InitialBufferAVolumes
For each member of BufferASelection, the measured volume immediately before the experiment was started.
Format: Multiple
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferBVolumes
For each member of BufferBSelection, the measured volume immediately before the experiment was started.
Format: Multiple
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
InitialBufferAAppearances
For each member of BufferASelection, an image taken immediately before the experiment was started.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
InitialBufferBAppearances
For each member of BufferBSelection, an image taken immediately before the experiment was started.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FlowInjectionPurgeCycle
Determines whether to have the sample replaced back into the origin container from the purge syringing.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
InjectionTable
The sequence of samples injected for a given experiment run including for ColumnPrime, SamplesIn, Standards, Blanks, and ColumnFlush.
Format: Multiple
[Sample]
Header: Sample
Class: Link
[Type]
Header: Type
Class: Expression
[Gradient]
Header: Gradient
Class: Link
Relation: Object[Method]
[InjectionVolume]
Header: InjectionVolume
Class: Real
Unit: 1 microliter
[DilutionFactor]
Header: DilutionFactor
Class: Real
[ColumnTemperature]
Header: ColumnTemperature
Class: Real
Unit: 1 degree Celsius
[FractionCollectionMethod]
Header: FractionCollectionMethod
Class: Link
Relation: Object[Method]
[Data]
Header: Data
Class: Link
Relation: Object[Data]
SampleLoopDisconnects
For each member of SamplesIn, if injecting by autosampler, specifies the volume of initial buffer that is flowed through the sample loop to displace the sample, before the sample loop is disconnected from the flow path and the user specified gradient begins. A Null value indicates that the sample loop remains connected for the duration of the run.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
FractionCollectionModes
For each member of SamplesIn, the mode by which fractions are collected, based either on always collecting peaks in a given time range, collecting peaks when ever absorbance crosses above a threshold value, or based on the steepness of a peak's slope.
Format: Multiple
Class: Expression
Programmatic Pattern: FractionCollectionModeP
FractionCollectionStartTimes
For each member of SamplesIn, the time before which no fractions will be collected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
FractionCollectionEndTimes
For each member of SamplesIn, the time after which no fractions will be collected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
MaxFractionVolumes
For each member of SamplesIn, the maximum volume to be collected in a single fraction after which a new fraction will be generated.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Liter Milli]
MaxCollectionPeriods
For each member of SamplesIn, the maximum amount to collect in a single fraction after which a new fraction will be generated when FractionCollectionMode is Time.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterP[0 Second]
PeakMinimumDurations
For each member of SamplesIn, the least of amounts of time that changes in slopes must be maintained before they are registered.
Format: Multiple
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
AbsoluteThresholds
For each member of SamplesIn, the absorbance or conductivity signal value above which fractions will always be collected when in Threshold mode.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Millisiemen
Hold[GreaterEqualP[0 AbsorbanceUnit Milli] | GreaterEqualP[0 -----------] | UnitsP[0 Unit]]
Centimeter
PeakEndThresholds
For each member of SamplesIn, the absorbance or conductivity signal value below which the end of a peak is marked and fraction collection stops.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Millisiemen
Hold[GreaterEqualP[0 AbsorbanceUnit Milli] | GreaterEqualP[0 -----------] | UnitsP[0 Unit]]
Centimeter
PeakSlopes
For each member of SamplesIn, the minimum slope rate (per second) that must be met before a peak is detected and fraction collection begins. A new peak (and new fraction) can be registered once the slope drops below this again, and collection ends when the PeakEndThreshold value is reached.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Milli Millisiemen Unit
Hold[GreaterEqualP[0 AbsorbanceUnit ------] | GreaterEqualP[0 -----------------] | UnitsP[0 ------]]
Second Centimeter Second Second
PeakSlopeEnds
For each member of SamplesIn, the slope rate (per second) that indicates when to end Peak-based fraction collections.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: Milli Millisiemen Unit
Hold[GreaterEqualP[0 AbsorbanceUnit ------] | GreaterEqualP[0 -----------------] | UnitsP[0 ------]]
Second Centimeter Second Second
Wavelength
The wavelength of light absorbed in the detector's flow cell.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nano Meter]
Wavelengths
The wavelength(s) of light absorbed in the detector's flow cell.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
FinalBufferAVolumes
For each member of BufferASelection, the measured volume immediately after the experiment was finished.
Format: Multiple
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferBVolumes
For each member of BufferBSelection, the measured volume immediately after the experiment was finished.
Format: Multiple
Class: Real
Unit: 1 liter
Programmatic Pattern: GreaterEqualP[0 Liter]
FinalBufferAAppearances
For each member of BufferASelection, an image of the corresponding BufferA taken immediately after the experiment was finished.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
FinalBufferBAppearances
For each member of BufferBSelection, an image of the corresponding taken immediately after the experiment was finished.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
MethodFiles
The raw instrument files imported into the Instrument software to conduct the run.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
SampleTemperature
The nominal temperature of the autosampler compartment prior to and during a run.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
SampleVolumes
For each member of SamplesIn, the volume taken from the sample and injected onto the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
SampleFlowRate
For each member of SamplesIn, the total rate of sample pumped onto the Column when InjectionType -> FlowInjection or Superloop.
Format: Multiple
Class: Real
Unit: 1 milliliter per minute
Programmatic Pattern: 0 Milli Liter
Hold[GreaterEqualP[-------------]]
Minute
PlateSeal
The package of pierceable plate seals used to cover plates of injection samples in this experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
StandardData
For each Standard, the chromatography trace generated.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
BlankData
For each member of Blanks, the chromatography trace generated for the blank's injection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
PrimeData
Chromatography data generated for any column prime runs.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
FlushData
Chromatography data generated for any column flush runs.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
TubingRinseSolution
The solution used to rinse buffers lines.
Format: Single
Class: Link
Programmatic Pattern: _Link
CapWashAdapter
Buffer cap plumbing adapter used to clean the tubing interior of used buffer caps.
Format: Single
Class: Link
Programmatic Pattern: _Link
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ReadyCheckComputations
The list of manifold job notebooks that was used to evaluate ReadyCheck for this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Job]
Gradient
BufferASelection
The unique list of all of the various mobile phase solutions used within BufferA/C/E/G.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferBSelection
The unique list of all of the various mobile phase solutions used within BufferB/D/F/H.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GradientA
For each member of SamplesIn, the percentage of BufferA in the composition over time, in the form: {Time, % BufferA} or a single % BufferA for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientB
For each member of SamplesIn, the percentage of BufferB in the composition over time, in the form: {Time, % BufferB} or a single % BufferB for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientC
For each member of SamplesIn, the percentage of BufferC in the composition over time, in the form: {Time, % BufferC} or a single % BufferA for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientD
For each member of SamplesIn, the percentage of BufferD in the composition over time, in the form: {Time, % BufferD} or a single % BufferD for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientE
For each member of SamplesIn, the percentage of BufferE in the composition over time, in the form: {Time, % BufferE} or a single % BufferE for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientF
For each member of SamplesIn, the percentage of BufferF in the composition over time, in the form: {Time, % BufferF} or a single % BufferF for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientG
For each member of SamplesIn, the percentage of BufferG in the composition over time, in the form: {Time, % BufferG} or a single % BufferG for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
GradientH
For each member of SamplesIn, the percentage of BufferH in the composition over time, in the form: {Time, % BufferH} or a single % BufferH for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
PreInjectionEquilibration Times
For each member of SamplesIn, specifies the amount of time that buffer should be run through the system at the initial conditions before the sample is injected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
FlowRate
For each member of SamplesIn, the total rate of mobile phase pumped through the instrument.
Format: Multiple
Class: Expression
Programmatic Pattern: Milliliter 0 Milli Liter
Hold[ListableP[{GreaterEqualP[0 Minute], GreaterEqualP[0 ----------]}] | GreaterEqualP[-------------]]
Minute Minute
GradientMethods
The gradients used during the course of the run, in the order they will be run.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method, Gradient]
AnalyteGradientMethods
For each member of SamplesIn, the buffer gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
ColumnPrimeGradients
For each prime, the method used to describe the gradient used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
Fraction Collection
FractionCollectionMethods
For each member of SamplesIn, Fraction collection parameters used for any samples that require fraction collection.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
FractionCollectionTemperature
The nominal temperature of the fraction collection compartment during a run.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
FractionContainers
Fraction containers that collected samples during the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
FractionContainerRacks
Racks holding the fraction containers that collected samples during the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
FractionCollection
Determines if fraction collection was performed in the protocol.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Standards
Standards
Samples with known profiles used to calibrate peak integrations and retention times for a given run.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardSampleVolumes
For each member of Standards, the volume taken from the standard and injected onto the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
StandardGradientMethods
For each member of Standards, the method used to describe the gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
StandardSampleLoopDisconnects
For each member of Standards, if injecting by autosampler, specifies the volume of initial buffer that is flowed through the sample loop to displace the standard, before the sample loop is disconnected from the flow path and the user specified gradient begins. A Null value indicates that the sample loop remains connected for the duration of the run.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
StandardGradientA
For each member of Standards, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientB
For each member of Standards, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientC
For each member of Standards, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer C for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientD
For each member of Standards, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer D for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientE
For each member of Standards, the percentage of BufferE in the composition over time, in the form: {Time, % Buffer E} or a single % Buffer E for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientF
For each member of Standards, the percentage of BufferF in the composition over time, in the form: {Time, % Buffer F} or a single % Buffer F for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientG
For each member of Standards, the percentage of BufferG in the composition over time, in the form: {Time, % Buffer G} or a single % Buffer G for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardGradientH
For each member of Standards, the percentage of BufferH in the composition over time, in the form: {Time, % Buffer H} or a single % Buffer H for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
StandardPreInjection EquilibrationTimes
For each member of Standards, specifies the amount of time that buffer should be run through the system at the initial conditions before the sample is injected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
StandardsStorageConditions
For each member of Standards, the storage conditions under which the standard samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
FlushGradientMethods
For each flush and prime, the method used to describe the gradient used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
Blanking
Blanks
Samples with known profiles used to calibrate background signal.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BlankSampleVolumes
For each member of Blanks, the volume taken from the blank and injected onto the column.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
BlankGradientMethods
For each member of Blanks, the method used to describe the gradient used for purification.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
BlankSampleLoopDisconnects
For each member of Blanks, if injecting by autosampler, specifies the volume of initial buffer that is flowed through the sample loop to displace the blank, before the sample loop is disconnected from the flow path and the user specified gradient begins. A Null value indicates that the sample loop remains connected for the duration of the run.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
BlankGradientA
For each member of Blanks, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientB
For each member of Blanks, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientC
For each member of Blanks, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientD
For each member of Blanks, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientE
For each member of Blanks, the percentage of BufferE in the composition over time, in the form: {Time, % Buffer E} or a single % Buffer E for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientF
For each member of Blanks, the percentage of BufferF in the composition over time, in the form: {Time, % Buffer F} or a single % Buffer F for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientG
For each member of Blanks, the percentage of BufferG in the composition over time, in the form: {Time, % Buffer G} or a single % Buffer G for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankGradientH
For each member of Blanks, the percentage of BufferH in the composition over time, in the form: {Time, % Buffer H} or a single % Buffer H for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
BlankPreInjection EquilibrationTimes
For each member of Blanks, specifies the amount of time that buffer should be run through the system at the initial conditions before the sample is injected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
BlanksStorageConditions
For each member of Blanks, the storage conditions under which the blank samples should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Column Prime
ColumnPrimeGradientA
For each column prime, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientB
For each column prime, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientC
For each column prime, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientD
For each column prime, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientE
For each column prime, the percentage of BufferE in the composition over time, in the form: {Time, % Buffer E} or a single % Buffer E for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientF
For each column prime, the percentage of BufferF in the composition over time, in the form: {Time, % Buffer F} or a single % Buffer F for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientG
For each column prime, the percentage of BufferG in the composition over time, in the form: {Time, % Buffer G} or a single % Buffer G for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimeGradientH
For each column prime, the percentage of BufferH in the composition over time, in the form: {Time, % Buffer H} or a single % Buffer H for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnPrimePreInjection EquilibrationTimes
For each column prime, specifies the amount of time that buffer should be run through the system at the initial conditions before the sample is injected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
Column Flush
ColumnFlushGradients
For each flush, the method used to describe the gradient used.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Method]
ColumnFlushGradientA
For each column flush, the percentage of BufferA in the composition over time, in the form: {Time, % Buffer A} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientB
For each column flush, the percentage of BufferB in the composition over time, in the form: {Time, % Buffer B} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientC
For each column flush, the percentage of BufferC in the composition over time, in the form: {Time, % Buffer C} or a single % Buffer A for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientD
For each column flush, the percentage of BufferD in the composition over time, in the form: {Time, % Buffer D} or a single % Buffer B for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientE
For each column flush, the percentage of BufferE in the composition over time, in the form: {Time, % Buffer E} or a single % Buffer E for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientF
For each column flush, the percentage of BufferF in the composition over time, in the form: {Time, % Buffer F} or a single % Buffer F for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientG
For each column flush, the percentage of BufferG in the composition over time, in the form: {Time, % Buffer G} or a single % Buffer G for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushGradientH
For each column flush, the percentage of BufferH in the composition over time, in the form: {Time, % Buffer H} or a single % Buffer H for the entire run.
Format: Multiple
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Minute], RangeP[0 Percent, 100 Percent]}...} | RangeP[0 Percent, 100 Percent]
ColumnFlushPreInjection EquilibrationTimes
For each column flush, specifies the amount of time that buffer should be run through the system at the initial conditions before the sample is injected.
Format: Multiple
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Minute]
Last modified on Sun 20 Nov 2022 1:34:22