Object[Protocol, qPCR]
A protocol for quantifying nucleic acids using a polymerase chain reaction in conjunction with fluorescence readouts.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, qPCR, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Protocol, qPCR]
Pattern Description: Object[Protocol, qPCR]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The investigator who created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User][ProtocolsAuthored]
General
DateConfirmed
The date on which the protocol first entered processing or a backlog.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateEnqueued
The date on which the protocol was accepted and added to the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateStarted
The date on which the protocol moved from the front of the queue to begin running in the lab.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCompleted
The date on which the last step of this protocol's execution was finished.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
DateCanceled
The date on which the protocol was canceled and removed from the queue.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Script
The script that created this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook][Protocols]
SubprotocolScripts
All scripts generated by this protocol as a list of subprotocols.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Notebook, Script][ParentProtocol]
Site
The ECL site at which this protocol was executed.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Site]
SamplesIn
Input samples for this analytical or preparative experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersIn
Any containers containing this protocols' SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
NumberOfReplicates
Indicates the number of times the experiment is repeated with identical parameters.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
Status
The current status of the protocol.
Format: Single
Class: Expression
Programmatic Pattern: ProtocolStatusP
CheckpointProgress
A listing of all the checkpoints passed in the execution of this protocol.
Format: Multiple
[[1]] Name
Header: Name
Class: String
Programmatic Pattern: _String
[[2]] Start Time
Header: Start Time
Class: Date
Programmatic Pattern: _?DateObjectQ
[[3]] End Time
Header: End Time
Class: Date
Programmatic Pattern: _?DateObjectQ|Null
SubprotocolDescription
A short title indicating what this subprotocol accomplishes.
Format: Single
Class: String
Programmatic Pattern: _String
BatchedUnitOperations
The individual batches of the protocol, as they will be executed in the lab.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[UnitOperation][Protocol]
ReplacementProtocol
If this protocol was aborted due to technical reasons, links to the replacement protocol run in its place.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sterile
Indicates if the protocol is performed in a sterile environment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
LiquidHandlingLog
The instrumentation trace file that monitored and recorded the execution of this robotic liquid handling.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
PooledSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
NestedIndexMatchingSamplesIn
The members of SamplesIn gathered into the pools that are mixed in each AliquotContainer.
Format: Multiple
Class: Expression
Programmatic Pattern: {ObjectReferenceP[Object[Sample]]..}
InitialNitrogenPressure
The pressure data of the nitrogen gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
NitrogenPressureLog
The pressure log for the nitrogen gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialCO2Pressure
The pressure data of the CO2 gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
CO2PressureLog
The pressure log for the CO2 gas source for the relevant section of the facility.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
InitialArgonPressure
The pressure data of the argon gas source before starting the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
HoldOrder
Indicates if the queue position of this protocol should be strictly enforced, regardless of the available resources in the lab.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
Priority
Indicates if this protocol should be prioritized to start at the given StartDate, have dedicated operation for its entirety, and not be interrupted by other protocols.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
StartDate
The date at which this protocol should be prioritized to start.
Format: Single
Class: Date
Programmatic Pattern: _?DateObjectQ
Branch
The git branch that this protocol should be run on.
Format: Single
Class: String
Programmatic Pattern: _String
Instrument
The instrument used to rapidly cycle temperatures and record fluorescence emitted by dyes or probes.
Format: Single
Class: Link
Programmatic Pattern: _Link
RunTime
The length of time for which the thermal cycler is expected to run given the specified parameters.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Second]
ReactionVolume
The total volume of the reaction including the sample, primers, probes, dyes, master mix and buffer.
Format: Single
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
MasterMix
The master mixes of polymerase, nucleobases, and buffer that this experiment uses to amplify target oligomers.
Format: Single
Class: Link
Programmatic Pattern: _Link
MasterMixVolume
The portion of the ReactionVolume volume that is composed of MasterMix.
Format: Single
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
Buffer
The sample used to bring each reaction to its ReactionVolume once all other components have been added and to perform all dilutions during the setup of this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Replicate Experiments
Template
The protocol whose option selections should be selected as the default options for this protocol.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][ProtocolsTemplated]
ProtocolsTemplated
Protocols which were generated using this protocol's options as a starting point for its option defaults.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Template]
Sample Preparation
PreparatoryUnitOperations
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
PreparatoryPrimitives
A list of transfers, consolidations, aliquots, mixes and dilutions that will be performed in the order listed to prepare samples for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: _ | _
VolumeCheckSamplePrep
Indicates if any liquid samples prepared by using the PreparatoryUnitOperations option should have their volumes measured and updated before running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
IncubateSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be heated prior to any aliquoting or the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
CentrifugeSamplePreparation
For each member of SamplesIn, parameters describing how the input samples should be spun down prior to any aliquoting or the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilterSamplePreparation
For each member of SamplesIn, parameters describing how particulates should be separated from the input samples prior to any aliquoting or the start of the experiment.
Format: Multiple
[Filtration]
Header: Filtration
Class: Boolean
[FiltrationType]
Header: FiltrationType
Class: Expression
[Filter]
Header: Filter
Class: Link
Relation: Object[Container] | Model[Container] | Object[Sample] | Model[Sample] | Object[Item] | Model[Item]
[FilterMaterial]
Header: FilterMaterial
Class: Expression
[FilterPoreSize]
Header: FilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterContainerOut]
Header: FilterContainerOut
Class: Expression
[FilterInstrument]
Header: FilterInstrument
Class: Link
Relation: Model[Instrument, FilterBlock] | Object[Instrument, FilterBlock] | Model[Instrument, PeristalticPump] | Object[Instrument, PeristalticPump] | Model[Instrument, VacuumPump] | Object[Instrument, VacuumPump] | Model[Instrument, Centrifuge] | Object[Instrument, Centrifuge] | Model[Instrument, SyringePump] | Object[Instrument, SyringePump]
[FilterSyringe]
Header: FilterSyringe
Class: Link
[FilterHousing]
Header: FilterHousing
Class: Link
[FilterIntensity]
Header: FilterIntensity
Class: VariableUnit
[FilterTime]
Header: FilterTime
Class: Real
Unit: 1 minute
[FilterTemperature]
Header: FilterTemperature
Class: Real
Unit: 1 degree Celsius
[FilterSterile]
Header: FilterSterile
Class: Boolean
[FilterAliquotContainer]
Header: FilterAliquotContainer
Class: Expression
[FilterAliquot]
Header: FilterAliquot
Class: Real
Unit: 1 liter
[PrefilterMaterial]
Header: PrefilterMaterial
Class: Expression
[PrefilterPoreSize]
Header: PrefilterPoreSize
Class: Real
Unit: 1 micrometer
[FilterAliquotDestinationWell]
Header: FilterAliquotDestinationWell
Class: Expression
SamplePreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the SamplesIn prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
PooledIncubateSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
NestedIndexMatchingIncubate SamplePreparation
For each member of NestedIndexMatchingSamplesIn, parameters describing how the pooled samples should be incubated after aliquoting but prior to the start of the experiment.
Format: Multiple
[Incubate]
Header: Incubate
Class: Boolean
[IncubationTemperature]
Header: IncubationTemperature
Class: Real
Unit: 1 degree Celsius
[IncubationTime]
Header: IncubationTime
Class: Real
Unit: 1 second
[Mix]
Header: Mix
Class: Boolean
[MixType]
Header: MixType
Class: Expression
[MixUntilDissolved]
Header: MixUntilDissolved
Class: Boolean
[MaxIncubationTime]
Header: MaxIncubationTime
Class: Real
Unit: 1 second
[IncubationInstrument]
Header: IncubationInstrument
Class: Link
[AnnealingTime]
Header: AnnealingTime
Class: Real
Unit: 1 second
[IncubateAliquotContainer]
Header: IncubateAliquotContainer
Class: Expression
[IncubateAliquot]
Header: IncubateAliquot
Class: Real
Unit: 1 liter
[IncubateAliquotDestinationWell]
Header: IncubateAliquotDestinationWell
Class: Expression
PooledCentrifugeSample Preparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
NestedIndexMatchingCentrifuge SamplePreparation
For each member of PooledSamplesIn, parameters describing how the pooled samples should be centrifuge after aliquoting and pooled mixing but prior to the start of the experiment.
Format: Multiple
[Centrifuge]
Header: Centrifuge
Class: Boolean
[CentrifugeInstrument]
Header: CentrifugeInstrument
Class: Link
[CentrifugeIntensity]
Header: CentrifugeIntensity
Class: VariableUnit
[CentrifugeTime]
Header: CentrifugeTime
Class: Real
Unit: 1 minute
[CentrifugeTemperature]
Header: CentrifugeTemperature
Class: Real
Unit: 1 degree Celsius
[CentrifugeAliquotContainer]
Header: CentrifugeAliquotContainer
Class: Expression
[CentrifugeAliquot]
Header: CentrifugeAliquot
Class: Real
Unit: 1 liter
[CentrifugeAliquotDestinationWell]
Header: CentrifugeAliquotDestinationWell
Class: Expression
FilteredSamples
Samples corresponding to filtered eluents of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
Aliquoting
AliquotSamplePreparation
For each member of AliquotSamples, parameters describing how aliquots should be drawn from the input samples after initial sample preparation in order to create new aliquot samples upon which aliquot preparation and the experiment should proceed.
Format: Multiple
[Aliquot]
Header: Aliquot
Class: Boolean
[AliquotAmount]
Header: AliquotAmount
Class: Expression
[TargetConcentration]
Header: TargetConcentration
Class: Expression
[AssayVolume]
Header: AssayVolume
Class: Real
Unit: 1 milliliter
[AliquotContainer]
Header: AliquotContainer
Class: Expression
[AssayBuffer]
Header: AssayBuffer
Class: Link
[BufferDiluent]
Header: BufferDiluent
Class: Link
[BufferDilutionFactor]
Header: BufferDilutionFactor
Class: Real
[ConcentratedBuffer]
Header: ConcentratedBuffer
Class: Link
[DestinationWell]
Header: DestinationWell
Class: Expression
[TargetConcentrationAnalyte]
Header: TargetConcentrationAnalyte
Class: Expression
[AliquotSampleLabel]
Header: AliquotSampleLabel
Class: String
AliquotProtocols
Protocols used to create AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotPreparationProtocols
Protocols used to mix, centrifuge, incubate, and filter the AliquotSamples prior to starting the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
AliquotSamples
Samples corresponding to aliquots of SamplesIn generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
AliquotContainers
Containers that hold AliquotSamples generated during sample preparation and intended for use in this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SaveAliquots
Indicates if the AliquotSamples should be stored after the experiment, rather than being discarded.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ConsolidateAliquots
Indicates if identical aliquots should be consolidated in a single sample.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
AliquotPreparation
Indicates if the aliquotting should occur manually or on a robotic liquid handler.
Format: Single
Class: Expression
Programmatic Pattern: PreparationMethodP
AliquotVolumes
For each member of SamplesIn, the volume transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterP[0 Micro Liter]
AliquotMasses
For each member of SamplesIn, the mass of a solid transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 milligram
Programmatic Pattern: GreaterP[0 Milligram]
AliquotAmounts
For each member of SamplesIn, the amount transferred from the sample into the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: VariableUnit
Programmatic Pattern: GreaterP[0 Milliliter] | GreaterP[0 Milligram] | GreaterP[0 Unit, 1 Unit]
TargetConcentrations
For each member of SamplesIn, the final concentration of analyte in the aliquot sample which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: Milligram
Hold[GreaterP[0 Micro Molar] | GreaterP[0 ----------]]
Milliliter
AssayVolumes
For each member of SamplesIn (or PooledSamplesIn), the total volume of the reaction mixture which is used in lieu of the initial sample for the experiment.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Micro Liter]
AssayBuffers
For each member of SamplesIn (or PooledSamplesIn), the final buffers used to generate AliquotSamples by aliquoting SamplesIn.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffers
For each member of SamplesIn (or PooledSamplesIn), the concentrated buffer source which is added to the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactors
For each member of SamplesIn (or PooledSamplesIn), he dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[0]
BufferDiluents
For each member of SamplesIn (or PooledSamplesIn), the diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ConcentratedBuffer
The concentrated buffer source which is added to each of the AliquotSamples to obtain 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
BufferDilutionFactor
The dilution factor by which the concentrated buffer is diluted in preparing the AliquotSamples to obtain a 1x buffer concentration after dilution of the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0]
BufferDiluent
The diluent used to dilute the concentration of the concentrated buffer in preparing the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayBuffer
The desired buffer for the AliquotSamples which are used in lieu of the SamplesIn for the experiment.
Format: Single
Class: Link
Programmatic Pattern: _Link
Sample Post-Processing
PostProcessingProtocols
Protocols used to perform post-processing such as sample imaging or volume measurement.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol]
ImageSample
Indicates if the samples that are modified in the course of the experiment are imaged after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureVolume
Indicates if the volume of the liquid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeasureWeight
Indicates if the weight of the solid samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Measure
Indicates if the volume/weight of the samples modified in the course of the experiment are measured after running the experiment.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
Storage
Indicates the conditions under which any samples generated by this experiment are stored after the protocol is completed.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Experimental Results
Data
Any primary data generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
SamplesOut
Any preparative samples generated by the protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ContainersOut
Any containers holding SamplesOut generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EnvironmentalData
Measurements of environmental conditions (temperature and humidity) recorded during the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
StandardData
The standard curve data generated as a part of this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
BlankData
The no-template control data generated as a part of this experiment.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Protocol]
MethodFile
The file containing parameters dictating how the instrument should perform the qPCR run.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
DataFile
The file containing data collected during the qPCR run.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Troubleshooting
TroubleshootingReports
Reports for errors that require troubleshooting associated with the execution of this top-level protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Resources
AwaitingResources
Indicates if this protocol is awaiting the arrival of items before it can be begun or continued.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ShippingMaterials
Indicates the transactions, and the specific products or samples therein, that must be received before this protocol can be begun or continued.
Format: Multiple
[[1]] Transaction
Header: Transaction
Class: Link
Programmatic Pattern: _Link
[[2]] Required Product
Header: Required Product
Class: Link
Programmatic Pattern: _Link
HazardousWaste
The types of waste generated by this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
DispenserContainers
Containers gathered as destinations for any resources obtained from a chemical dispenser.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
GasSources
A list of containers that supplied gas to instruments in this protocol through plumbing connections.
Format: Multiple
[[1]] Gas Type
Header: Gas Type
Class: Expression
Programmatic Pattern: GasP
[[2]] Source Container
Header: Source Container
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container]
[[3]] Supplied Instrument
Header: Supplied Instrument
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Cleaning
WasteGenerated
For each type of waste generated by this protocol, the total amount (in grams) generated during the conduct of the protocol.
Format: Multiple
[Waste]
Header: Waste
Class: Link
Relation: Model[Sample]
[Weight]
Header: Weight
Class: Real
Unit: 1 gram
Storage Information
StoragePrice
The total monthly price for warehousing all user owned items associated with this protocol in an ECL facility under the storage conditions specified by each item.
Format: Single
Class: Real
Unit: 1 US dollar per month
Programmatic Pattern: USD
Hold[GreaterEqualP[0 -----]]
Month
Sample Storage
SamplesInStorage
For each member of SamplesIn, The storage conditions under which any input samples to this experiment should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AliquotStorage
For each member of AliquotSamples, The storage conditions under which any aliquot samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
SamplesOutStorage
For each member of SamplesOut, the storage conditions under which any output samples generated by this experiment should be stored after the protocol is completed.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ForwardPrimerStorage Conditions
For each member of SamplesIn, the storage conditions under which the forward primers should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: {SampleStorageTypeP | Disposal..}
ReversePrimerStorage Conditions
For each member of SamplesIn, the storage conditions under which the reverse primers should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: {SampleStorageTypeP | Disposal..}
ProbeStorageConditions
For each member of SamplesIn, the storage conditions under which the probes should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: {SampleStorageTypeP | Disposal..}
StandardStorageConditions
For each member of Standards, the storage conditions under which the standards should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardForwardPrimerStorage Conditions
For each member of Standards, the storage conditions under which the forward primers for the standards should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardReversePrimerStorage Conditions
For each member of Standards, the storage conditions under which the reverse primers for the standards should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
StandardProbeStorage Conditions
For each member of Standards, the storage conditions under which the probes for the standards should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
EndogenousForwardPrimer StorageConditions
For each member of SamplesIn, the storage conditions under which the endogenous forward primers should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
EndogenousReversePrimer StorageConditions
For each member of SamplesIn, the storage conditions under which the endogenous reverse primers should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
EndogenousProbeStorage Conditions
For each member of SamplesIn, the storage conditions under which the probes for the endogenous probes should be stored after their usage in this experiment.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
MasterMixStorageCondition
The storage conditions under which the master mix should be stored after their usage in this experiment.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
AssayPlateStorageCondition
The storage conditions under which the thermocycled assay plate should be stored after its usage in this experiment.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
ArrayCardStorageCondition
The storage conditions under which the thermocycled array card should be stored after its usage in this experiment.
Format: Single
Class: Expression
Programmatic Pattern: SampleStorageTypeP | Disposal
Option Handling
ResolvedOptions
The final options used as parameters for this protocol, after automatic options are resolved.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
UnresolvedOptions
The verbatim options originally input by the protocol author to generate this protocol, often with some options set to Automatic.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Computations
ParallelComputations
List of computational jobs generated and run in parallel to the execution of this protocol.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ComputationsOutstanding
Indicates if any of the computation jobs in ParallelComputations have not yet completed.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
ErroneousComputations
List of computational jobs generated and run in parallel to the execution of this protocol in which a warning or error was thown, or that did not finish.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Sample Loading
AssayPlate
The 384-well qPCR plate that will be loaded with samples, primers, probes, dyes, master mix and buffer.
Format: Single
Class: Link
Programmatic Pattern: _Link
PlateSeal
The optically transparent self-adhesive seal used to cover the assay plate wells during thermal cycling.
Format: Single
Class: Link
Programmatic Pattern: _Link
AssayPlatePrimitives
The set of instructions specifying the the loading of a qPCR plate with samples, primers, probes, dyes, master mix and buffer.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
AssayPlateManipulation
The sample manipulation protocol used to load the qPCR plate generated as a result of execution of AssayPlatePrimitives.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol, SampleManipulation]
ArrayCardPreparatoryPlate
The 96-well PCR plate for the preparation of reaction mixtures consisting of samples, master mix, and buffer.
Format: Single
Class: Link
Programmatic Pattern: _Link
ArrayCardPreparatory Primitives
The set of instructions specifying the preparation of reaction mixtures consisting of samples, master mix, and buffer.
Format: Multiple
Class: Expression
Programmatic Pattern: SampleManipulationP
ArrayCardPreparatory Manipulation
The robotic sample manipulation protocol generated as a result of the execution of ArrayCardPreparatoryPrimitives.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol, SampleManipulation]
ArrayCard
The input array card containing pre-dried primers and probes that will be loaded with reaction mixtures.
Format: Single
Class: Link
Programmatic Pattern: _Link
ArrayCardLoadingPrimitives
The set of instructions specifying the loading of the array card with reaction mixtures and the subsequent centrifugation.
Format: Multiple
Class: Expression
Programmatic Pattern: SamplePreparationP
ArrayCardLoadingManipulation
The manual sample preparation protocol generated as a result of the execution of ArrayCardLoadingPrimitives.
Format: Single
Class: Link
Programmatic Pattern: _Link
SampleVolumes
For each member of SamplesIn, the portion of the reaction that is made up of the sample.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
BufferVolumes
For each member of SamplesIn, the portion of the reaction that is made up of any additional buffer not already part of the master mix.
Format: Multiple
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
MoatVolume
The volume which each moat is filled with in order to slow evaporation of inner assay samples.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
MoatBuffer
The sample each moat well is filled with in order to slow evaporation of inner assay samples.
Format: Single
Class: Link
Programmatic Pattern: _Link
MoatSize
The depth the moat extends into the assay plate.
Format: Single
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
Dye Assay
DuplexStainingDye
The fluorescence dye that intercalates into double stranded DNA and emits fluorescent light when excited with the appropriate wavelength of light.
Format: Single
Class: Link
Programmatic Pattern: _Link
DuplexStainingDyeVolume
The portion of the reaction volume that is made up of any additional duplex staining dye not already part of the master mix.
Format: Single
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
DuplexStainingDyeExcitation Wavelength
The wavelengths of light used to excite the duplex staining dye.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
DuplexStainingDyeEmission Wavelength
The wavelength of light collected from the excited duplex staining dye.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
Target Amplification
ForwardPrimers
For each member of SamplesIn, the forward primers that this experiment uses to amplify a target sequence from the sample.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
ReversePrimers
For each member of SamplesIn, the reverse primers that this experiment uses to amplify a target sequence from the sample.
Format: Multiple
Class: Expression
Programmatic Pattern: {_Link..}
ForwardPrimerVolumes
For each member of SamplesIn, the volume of primer mix added to the reaction volume.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Milliliter]..}
ReversePrimerVolumes
For each member of SamplesIn, the volume of primer mix added to the reaction volume.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Milliliter]..}
ForwardPrimerResources
The flattened list of the reverse primers that this experiment uses to amplify the primary target from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ReversePrimerResources
The flattened list of the reverse primers that this experiment uses to amplify the primary target from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Probe Assay
Probes
For each member of SamplesIn, the molecular probe(s) that this experiment uses to quantify the amount of target sequence.
Format: Multiple
Class: Expression
ProbeResources
The flattened list of the probes that this experiment uses to quantify the amount of target sequence.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
ProbeVolumes
For each member of SamplesIn, the volume of probe added to the reaction volume.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Milliliter]..}
ProbeFluorophores
For each member of SamplesIn, the fluorophore conjugated to each of the molecular probe(s) that this experiment uses to quantify the amount of target sequence.
Format: Multiple
Class: Expression
Programmatic Pattern: {LinkP[Model[Molecule]]..}
ProbeExcitationWavelengths
For each member of SamplesIn, the wavelengths of light used to excite the probes.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Nanometer]..}
ProbeEmissionWavelengths
For each member of SamplesIn, the wavelengths of light collected from the excited probes.
Format: Multiple
Class: Expression
Programmatic Pattern: {GreaterEqualP[0 Nanometer]..}
Passive Control
ReferenceDye
The passive fluorescence reference dye this protocol uses to normalize fluorescence background fluctuations during quantification.
Format: Single
Class: Link
Programmatic Pattern: _Link
ReferenceDyeVolume
The portion of the reaction volume that is made up of any additional duplex staining dye not already part of the master mix.
Format: Single
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
ReferenceDyeExcitation Wavelength
The wavelengths of light used to excite the reference staining dye.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
ReferenceDyeEmission Wavelength
The wavelength of light collected from the excited reference staining dye.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
Endogenous Control
EndogenousForwardPrimers
For each member of SamplesIn, the forward primers that this experiment uses to amplify an endogenous control target from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EndogenousReversePrimers
For each member of SamplesIn, the forward primers that this experiment uses to amplify an endogenous control target from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EndogenousForwardPrimer Volumes
For each member of SamplesIn, the volume of endogenous control forward primer added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
EndogenousReversePrimer Volumes
For each member of SamplesIn, the volume of endogenous control forward primer added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
EndogenousProbes
For each member of SamplesIn, the molecular probe that this experiment uses to quantify the amount of endogenous target sequence.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
EndogenousProbeVolumes
For each member of SamplesIn, the volume of endogenous probe mix added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
EndogenousProbeFluorophores
For each member of SamplesIn, the fluorophore conjugated to the molecular probe that this experiment uses to quantify the amount of endogenous control target sequence.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Model[Molecule]
EndogenousProbeExcitation Wavelengths
For each member of SamplesIn, the wavelength of light used to excite the endogenous probe (if any).
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
EndogenousProbeEmission Wavelengths
For each member of SamplesIn, the wavelength of light collected from the excited endogenous probe (if any).
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
Reverse Transcription
ReverseTranscription
Indicates if a one-step reverse transcription is performed in order to convert RNA input samples to cDNA.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ReverseTranscription Temperature
The temperature at which the reverse transcriptase converts source RNA material into cDNA.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ReverseTranscriptionTime
The time for which the reverse transcriptase is allowed to synthesis cDNA strands from their template RNA strands.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
ReverseTranscriptionRampRate
The rate at which the temperature changes in degrees per second when bringing the samples up to reverse transcription temperature.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
Polymerase Activation
Activation
Indicates if hot start activation of the reaction is performed in order to remove thermolabile inhibiting conjugates from the polymerases.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
ActivationTemperature
The temperature of the initial activation step used to denature the strands in the reaction and to activate the polymerase.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ActivationTime
The time of the initial activation step used to denature the strands in the reaction and to activate the polymerase.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
ActivationRampRate
The rate at which the temperature changes in degrees per second between when bringing the samples down to activation temperature.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
Denaturation
DenaturationTemperature
The temperature used to denature all strands in the reaction in the first stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
DenaturationTime
The time used to denature all strands in the reaction in the first stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
DenaturationRampRate
The rate at which the temperature changes in degrees per second when bringing the samples up to denaturation temperature.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
Annealing
PrimerAnnealing
Indicates if primer annealing is performed as a separate step from strand extension.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
PrimerAnnealingTemperature
The temperature at which primers and probe are bound to the target strands for amplification in the second stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
PrimerAnnealingTime
The time for which the primers and probe are allowed to bind to the target strands for amplification in the second stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
PrimerAnnealingRampRate
The rate at which the temperature changes in degrees per second when bringing the samples up to annealing temperature.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
Extension
ExtensionTemperature
The temperature at which the polymerase extends the newly amplified strand from the annealed primers in the third stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
ExtensionTime
The time for which the polymerase is allowed to extend the newly amplified strand from the annealed primers in the third stage of the PCR cycle.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
ExtensionRampRate
The rate at which the temperature changes in degrees per second between when brining the samples up to extension temperature.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
NumberOfCycles
The number of cycles of denaturation, annealing, and extension to perform.
Format: Single
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
Melting Curve
MeltingCurve
Indicates if a melting curved should be created at the end of the experiment by recording the fluorescene of each sample while slowly dissasociating the strands.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
MeltingCurveTime
The length of time it should take to change the temperature from the MeltingCurveStartTemperature to MeltingCurveEndTemperature.
Format: Single
Class: Real
Unit: 1 minute
Programmatic Pattern: GreaterEqualP[0 Second]
MeltingCurveStartTemperature
The temperature at which the samples should be before starting the melting curve.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
MeltingCurveEndTemperature
The temperature, which when reached, should end the melting curve.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterEqualP[0 Kelvin]
PreMeltingCurveRampRate
The rate at which the temperature changes in degrees per second between the end of amplification and start of the melting curve.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
MeltingCurveRampRate
The rate at which the temperature changes in degrees per second between the start and end of the melting curve.
Format: Single
Class: Real
Unit: 1 degree Celsius per second
Programmatic Pattern: 0 Celsius
Hold[GreaterEqualP[---------]]
Second
Standard Curve
StandardVolume
The portion of each standard curve reaction volume that is made up of the standard sample.
Format: Single
Class: Real
Unit: 1 milliliter
Programmatic Pattern: GreaterEqualP[0 Milliliter]
Standards
The samples used to constructed a fitted line to determine absolute or relative amounts of unknown sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardConcentrations
For each member of Standards, the concentration of each step in the serial dilution if the concentration of the starting sample is known.
Format: Multiple
Class: Expression
Programmatic Pattern: {ConcentrationP..}
SerialDilutionFactors
For each member of Standards, the factor by which each subsequent sample in the serial dilution should be diluted.
Format: Multiple
Class: Real
Programmatic Pattern: GreaterP[1]
NumberOfDilutions
For each member of Standards, the number of serial dilutions in the whole series, including the initial (undiluted) standard sample.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
NumberOfStandardReplicates
For each member of Standards, the number of times the standard curve should be replicated.
Format: Multiple
Class: Integer
Programmatic Pattern: GreaterEqualP[0, 1]
StandardForwardPrimers
For each member of Standards, the forward primer that this experiment uses to amplify the standard target sequence from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardReversePrimers
For each member of Standards, the reverse primer that this experiment uses to amplify the standard target sequence from the sample.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardForwardPrimerVolumes
For each member of Standards, the volume of forward primer added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardReversePrimerVolumes
For each member of Standards, the volume of reverse primer added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardProbes
For each member of Standards, the molecular probe that this experiment uses to quantify the amount of standard target sequence.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
StandardProbeVolumes
For each member of Standards, the volume of standard probe added to the reaction volume.
Format: Multiple
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
StandardProbeFluorophores
For each member of Standards, the fluorophore conjugated to the molecular probe that this experiment uses to quantify the amount of standard target sequence.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Model[Molecule]
StandardProbeExcitation Wavelengths
For each member of Standards, the wavelengths of light used to excite the standard probes.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
StandardProbeEmission Wavelengths
For each member of Standards, the wavelength of light collected from the excited standard probes.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
Analysis & Reports
QuantificationCycleAnalyses
The analysis object(s) containing the quantification cycle calculation results.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
CopyNumberAnalyses
The analysis object(s) containing the copy number calculation results. Each copy number is determined from a standard curve of quantification cycle vs Log10 copy number.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis, CopyNumber][Protocol]
Last modified on Mon 26 Sep 2022 15:43:51