Object[Analysis, QuantificationCycle]
Determination of the amplification cycle at which the fluorescence in a quantitative polymerase chain reaction (qPCR) can be detected above the background fluorescence, which is defined as either the point at which the amplification curve crosses the target-specific threshold (Threshold method) or the inflection point of the amplification curve (InflectionPoint method).
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Analysis, QuantificationCycle, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Analysis, QuantificationCycle]
Pattern Description: Object[Analysis, QuantificationCycle]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Author
The person who ran the analysis.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[User]
General
UnresolvedOptions
The initial options entered into the analysis function that generated this object, along with the default values for any unspecified options.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
ResolvedOptions
The final options used by the analysis function, after automatic options have been determined from the input and unresolved options.
Format: Single
Class: Compressed
Programmatic Pattern: {_Rule...}
Reference
The objects containing the source data used in this analysis.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification, PipettingLinearity][FittingAnalysis] | Object[Data, AbsorbanceSpectroscopy][AbsorbanceSpectrumPeaksSource] | Object[Data, AbsorbanceSpectroscopy][QuantificationAnalyses] | Object[Data, AbsorbanceSpectroscopy][SmoothingAnalyses] | Object[Data, AbsorbanceIntensity][QuantificationAnalyses] | Object[Data, AgaroseGelElectrophoresis][SampleElectropherogramPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][MarkerElectropherogramPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][PostSelectionPeaksAnalyses] | Object[Data, AgaroseGelElectrophoresis][SmoothingAnalyses] | Object[Data, Appearance, Colonies][ColonyAnalysis] | Object[Data, MeltingCurve][MeltingAnalyses] | Object[Data, MeltingCurve][FluorescenceSpectraAnalyses] | Object[Data, MeltingCurve][AggregationAnalyses] | Object[Data, MeltingCurve][ThermalShiftAnalyses] | Object[Data, BioLayerInterferometry][QuantitationAnalysis] | Object[Data, BioLayerInterferometry][KineticsAnalysis] | Object[Data, BioLayerInterferometry][BinningAnalysis] | Object[Data, BioLayerInterferometry][DetectionLimitAnalysis] | Object[Data, CapillaryGelElectrophoresisSDS][LadderAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][SmoothingAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][PeaksAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][RelativeMigrationPeaksAnalyses] | Object[Data, CapillaryGelElectrophoresisSDS][FitAnalyses] | Object[Data, CapillaryIsoelectricFocusing][SmoothingAnalyses] | Object[Data, CapillaryIsoelectricFocusing][PeaksAnalyses] | Object[Data, CapillaryIsoelectricFocusing][FitAnalyses] | Object[Data, Chromatography][StandardAnalyses] | Object[Data, Chromatography][FractionPickingAnalysis] | Object[Data, Chromatography][ConductancePeaksAnalyses] | Object[Data, Chromatography][AbsorbancePeaksAnalyses] | Object[Data, Chromatography][SecondaryAbsorbancePeaksAnalyses] | Object[Data, Chromatography][Absorbance3DPeaksAnalyses] | Object[Data, Chromatography][FluorescencePeaksAnalyses] | Object[Data, Chromatography][SecondaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][TertiaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][QuaternaryFluorescencePeaksAnalyses] | Object[Data, Chromatography][ScatteringPeaksAnalyses] | Object[Data, Chromatography][FIDResponsePeaksAnalyses] | Object[Data, Chromatography][ChargePeaksAnalyses] | Object[Data, Chromatography][SmoothingAnalyses] | Object[Data, Chromatography][pHPeaksAnalyses] | Object[Data, Chromatography][RefractiveIndexPeaksAnalysis] | Object[Data, Chromatography][CircularDichroismPeaksAnalysis] | Object[Data, Chromatography][MultiAngleLightScatteringAnalyses] | Object[Data, Chromatography][DynamicLightScatteringAnalyses] | Object[Data, ChromatographyMassSpectra][IonAbundance3DPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][IonAbundancePeaksAnalyses] | Object[Data, ChromatographyMassSpectra][MassSpectrumPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][AbsorbancePeaksAnalyses] | Object[Data, ChromatographyMassSpectra][Absorbance3DPeaksAnalyses] | Object[Data, ChromatographyMassSpectra][DownsamplingAnalyses] | Object[Data, ChromatographyMassSpectra][SmoothingAnalyses] | Object[Data, CircularDichroism][SmoothingAnalyses] | Object[Data, CircularDichroism][StructureQuantificationAnalyses] | Object[Data, DifferentialScanningCalorimetry][HeatingCurvePeaksAnalyses] | Object[Data, DifferentialScanningCalorimetry][SmoothingAnalyses] | Object[Data, DNASequencing][SequenceAnalyses] | Object[Data, DynamicFoamAnalysis][BubbleRadiusAnalyses] | Object[Data, FlowCytometry][FlowCytometryAnalyses] | Object[Data, FluorescenceKinetics][RateFittingAnalyses] | Object[Data, FluorescencePolarizationKinetics][RateFittingAnalyses] | Object[Data, LuminescenceKinetics][RateFittingAnalyses] | Object[Data, FluorescenceSpectroscopy][EmissionSpectrumPeaksAnalyses] | Object[Data, FluorescenceSpectroscopy][ExcitationSpectrumPeaksAnalyses] | Object[Data, FluorescenceSpectroscopy][SmoothingAnalyses] | Object[Data, LuminescenceSpectroscopy][PeaksAnalyses] | Object[Data, LuminescenceSpectroscopy][SmoothingAnalyses] | Object[Data, MeltingCurve][InitialIntensityPeaksAnalyses] | Object[Data, MeltingCurve][FinalIntensityPeaksAnalyses] | Object[Data, MeltingCurve][InitialMassPeaksAnalyses] | Object[Data, MeltingCurve][FinalMassPeaksAnalyses] | Object[Data, MeltingCurve][DynamicLightScatteringAnalyses] | Object[Data, FluorescenceThermodynamics][MeltingAnalyses] | Object[Data, MassSpectrometry][MassSpectrumPeaksAnalyses] | Object[Data, MassSpectrometry][SmoothingAnalyses] | Object[Data, Microscope][AdjustmentAnalyses] | Object[Data, Microscope][CellCountAnalyses] | Object[Data, Microscope][MicroscopeOverlay] | Object[Data, ImageCells][CellCountAnalyses] | Object[Data, ImageCells][MicroscopeOverlay] | Object[Data, Nephelometry][QuantificationAnalyses] | Object[Data, NephelometryKinetics][GrowthCurveAnalyses] | Object[Data, NephelometryKinetics][SolubilityAnalyses] | Object[Data, NMR][NMRSpectrumPeaksAnalyses] | Object[Data, NMR][SmoothingAnalyses] | Object[Data, NMR2D][NMRSpectrumPeaksAnalyses] | Object[Data, PAGE][LanePeaksAnalyses] | Object[Data, PAGE][LadderAnalyses] | Object[Data, PAGE][SmoothingAnalyses] | Object[Data, qPCR][QuantificationCycleAnalyses] | Object[Data, TotalProteinDetection][FitSourceSpectra] | Object[Data, TotalProteinDetection][MassSpectrumPeaksAnalyses] | Object[Data, TotalProteinDetection][LadderAnalyses] | Object[Data, TLC][LanePeaksAnalyses] | Object[Data, TLC][SmoothingAnalyses] | Object[Data, Volume][QuantificationAnalyses] | Object[Data, Western][FitSourceSpectra] | Object[Data, Western][MassSpectrumPeaksAnalyses] | Object[Data, Western][LadderAnalyses] | Object[Data, Western][SmoothingAnalyses] | Object[Analysis, MeltingPoint][TopBaseline] | Object[Analysis, MeltingPoint][BottomBaseline] | Object[Analysis, MeltingPoint][Thermodynamics] | Object[Analysis, QuantificationCycle][CopyNumberAnalyses] | Object[Analysis, QuantificationCycle][StandardCurveAnalyses] | Object[Analysis, Composition][StandardCurveFitAnalyses] | Object[Analysis, Ladder][StandardFit] | Object[Analysis, Thermodynamics][Fit] | Object[Analysis, Fit][ParallelLineAnalyses] | Object[Data, XRayDiffraction][StructureAnalyses] | Object[Data, XRayDiffraction][DiffractionPeaksAnalyses] | Object[Data, XRayDiffraction][SmoothingAnalyses] | Object[Data, IRSpectroscopy][AbsorbanceSpectrumPeaksSource] | Object[Data, IRSpectroscopy][SmoothingAnalyses] | Object[Protocol, FlowCytometry][CompensationMatrixAnalyses] | Object[Protocol, TotalProteinQuantification][QuantificationAnalyses] | Object[Data, CircularDichroism][CircularDichroismPeaksAnalysis] | Object[Data, DynamicLightScattering][MassDistributionAnalyses] | Object[Data, DynamicLightScattering][IntensityDistributionAnalyses] | Object[Data, DynamicLightScattering][DynamicLightScatteringAnalyses] | Object[DesignOfExperiment][DesignOfExperimentAnalyses] | Object[Protocol, ThermalShift][DynamicLightScatteringLoadingAnalyses] | Object[Protocol, DynamicLightScattering][DynamicLightScatteringLoadingAnalyses] | Object[Data, DifferentialScanningCalorimetry][Analyses]
ReferenceField
The field in the Reference object containing the source data.
Format: Single
Class: Expression
Programmatic Pattern: FieldP[Output -> Short]
Protocol
The quantitative polymerase chain reaction (qPCR) protocol associated with the data used for analysis.
Format: Single
Class: Link
Programmatic Pattern: _Link
Method
The calculation method used for quantification cycle analysis.
Format: Single
Class: Expression
Programmatic Pattern: Threshold | InflectionPoint
Domain
The first and last cycle delimiting the range for analysis.
Format: Single
[[1]] Min
Header: Min
Class: Integer
Unit: 1 Cycles
Programmatic Pattern: GreaterP[0 Cycle]
[[2]] Max
Header: Max
Class: Integer
Unit: 1 Cycles
Programmatic Pattern: GreaterP[0 Cycle]
BaselineDomain
The first and last cycle delimiting the range of the baseline.
Format: Single
[[1]] Min
Header: Min
Class: Integer
Unit: 1 Cycles
Programmatic Pattern: GreaterP[0 Cycle]
[[2]] Max
Header: Max
Class: Integer
Unit: 1 Cycles
Programmatic Pattern: GreaterP[0 Cycle]
SmoothingRadius
The radius in cycles for GaussianFilter smoothing of the normalized and baseline-subtracted amplification curve, applicable to the InflectionPoint method.
Format: Single
Class: Integer
Unit: 1 Cycles
Programmatic Pattern: GreaterP[0 Cycle]
Template
The nucleic acid template used for target sequence amplification.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample]
ForwardPrimer
The forward primer used to amplify the target sequence, applicable to the Threshold method.
Format: Single
Class: Link
Programmatic Pattern: _Link
ReversePrimer
The reverse primer used to amplify the target sequence, applicable to the Threshold method.
Format: Single
Class: Link
Programmatic Pattern: _Link
Probe
The reporter probe (composed of a short, sequence-specific oligomer conjugated with a reporter and quencher) used to quantify the target sequence, applicable to the Threshold method.
Format: Single
Class: Link
Programmatic Pattern: _Link
FittingDataPoints
The processed amplification curve data points in Domain for interpolation (Threshold method) or logistic curve fitting (InflectionPoint method).
Format: Single
Class: QuantityArray
Unit: ['1 Cycles', '1 Rfus']
Programmatic Pattern: QuantityCoordinatesP[{Cycle, RFU}]
ExcitationWavelength
The excitation wavelength associated with the amplification curve.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
EmissionWavelength
The emission wavelength associated with the amplification curve.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Nanometer]
Threshold
The fluorescence signal significantly above baseline fluorescence at which amplification is considered to have begun, applicable to the Threshold method.
Format: Single
Class: Real
Unit: 1 Rfus
Programmatic Pattern: GreaterEqualP[0 RFU]
Analysis & Reports
AmplificationFit
The logistic curve fit to the amplification curve data points, applicable to the InflectionPoint method.
Format: Single
Class: Expression
Programmatic Pattern: _Function
QuantificationCycle
The amplification cycle at which the fluorescence in a quantitative polymerase chain reaction (qPCR) can be detected above the background fluorescence, determined using either the Threshold or InflectionPoint method.
Format: Single
Class: Real
Unit: 1 Cycles
Programmatic Pattern: GreaterEqualP[0 Cycle]
StandardCurveAnalyses
The linear fit analyses that use this quantification cycle analysis as a data point for fitting a standard curve.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
CopyNumberAnalyses
The copy number analyses based on this quantification cycle analysis.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:43