ecl-logo Documentation

Object[Data, Appearance, Colonies]

Image representing the physical appearance of a sample that contains bacterial colonies. Contains specific information regarding non-brightfield images of the colonies, including Fluorescent images, images from a BlueWhite Screen, and images taken with Darkfield lighting.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Appearance, Colonies, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, Appearance, Colonies]
    Pattern Description: Object[Data, Appearance, Colonies]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Method Information

    ContainersIn

    The containers that were imaged to generate this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Alternatives[Object[Container]]

    Camera

    The camera used to capture this image.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Part, Camera][Data]

    DateAcquired

    The date on which the image was acquired.
    Format: Single
    Class: Date
    Programmatic Pattern: _?DateObjectQ

    Software

    The acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

    SoftwareVersion

    Indicates the version of the acquisition software on the instrument used to acquire the images.
    Format: Single
    Class: String
    Programmatic Pattern: _String

Imaging Specifications

    FieldOfView

    The horizontal length of the uncropped image.
    Format: Single
    Class: Expression
    Programmatic Pattern: CameraFieldOfViewP

    ImagingDirection

    The direction from which the sample image was captured.
    Format: Single
    Class: Expression
    Programmatic Pattern: ImagingDirectionP

    IlluminationDirection

    The direction(s) from which the sample was illuminated.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: IlluminationDirectionP

    ExposureTime

    The length of time for which a camera shutter stayed open while taking this image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Second]

    VioletFluorescenceImageFile

    An image file containing the image of the sample while using a 337 Nanometer excitation wavelength and a 447 Nanometer wavelength emission filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    VioletFluorescenceExcitation Wavelength

    The wavelength of the excitation filter used to image the sample that corresponds to VioletFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    VioletFluorescenceEmission Wavelength

    The wavelength of the emission filter used to image the sample that corresponds to VioletFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    VioletFluorescenceExposure Time

    The length of time for which the sample was exposed to the VioletFluorescence channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    VioletFluorescence IlluminationDirection

    The direction from which the sample was illuminated with VioletFluorescenceExcitationWavelength.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Top

    VioletFluorescenceImageScale

    The scale in pixels/distance relating pixels of the image when the sample was illuminated with VioletFluorescenceExcitationWavelength to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    GreenFluorescenceImageFile

    An image file containing the image of the sample while using a 457 Nanometer excitation wavelength and a 536 Nanometer wavelength emission filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    GreenFluorescenceExcitation Wavelength

    The wavelength of the excitation filter used to image the sample that corresponds to GreenFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    GreenFluorescenceEmission Wavelength

    The wavelength of the emission filter used to image the sample that corresponds to GreenFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    GreenFluorescenceExposureTime

    The length of time for which the sample was exposed to the GreenFluorescence channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    GreenFluorescenceIllumination Direction

    The direction from which the sample was illuminated with GreenFluorescenceExcitationWavelength.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Top

    GreenFluorescenceImageScale

    The scale in pixels/distance relating pixels of the image when the sample was illuminated with GreenFluorescenceExcitationWavelength to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    OrangeFluorescenceImageFile

    An image file containing the image of the sample while using a 531 Nanometer excitation wavelength and a 593 Nanometer wavelength emission filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    OrangeFluorescenceExcitation Wavelength

    The wavelength of the excitation filter used to image the sample that corresponds to OrangeFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    OrangeFluorescenceEmission Wavelength

    The wavelength of the emission filter used to image the sample that corresponds to OrangeFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    OrangeFluorescenceExposure Time

    The length of time for which the sample was exposed to the OrangeFluorescence channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    OrangeFluorescence IlluminationDirection

    The direction from which the sample was illuminated with OrangeFluorescenceExcitationWavelength.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Top

    OrangeFluorescenceImageScale

    The scale in pixels/distance relating pixels of the image when the sample was illuminated with OrangeFluorescenceExcitationWavelength to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    RedFluorescenceImageFile

    An image file containing the image of the sample while using a 531 Nanometer excitation wavelength and a 624 Nanometer wavelength emission filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    RedFluorescenceExcitation Wavelength

    The wavelength of the excitation filter used to image the sample that corresponds to RedFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    RedFluorescenceEmission Wavelength

    The wavelength of the emission filter used to image the sample that corresponds to RedFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    RedFluorescenceExposureTime

    The length of time for which the sample was exposed to the RedFluorescence channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    RedFluorescenceIllumination Direction

    The direction from which the sample was illuminated with RedFluorescenceExcitationWavelength.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Top

    RedFluorescenceImageScale

    The scale in pixels/distance relating pixels of the image when the sample was illuminated with RedFluorescenceExcitationWavelength to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    DarkRedFluorescenceImageFile

    An image file containing the image of the sample while using a 628 Nanometer excitation wavelength and a 692 Nanometer wavelength emission filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    DarkRedFluorescenceExcitation Wavelength

    The wavelength of the excitation filter used to image the sample that corresponds to DarkRedFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    DarkRedFluorescenceEmission Wavelength

    The wavelength of the emission filter used to image the sample that corresponds to DarkRedFluorescence.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    DarkRedFluorescenceExposure Time

    The length of time for which the sample was exposed to the DarkRedFluorescence channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    DarkRedFluorescence IlluminationDirection

    The direction from which the sample was illuminated with DarkRedFluorescenceExcitationWavelength.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Top

    DarkRedFluorescenceImageScale

    The scale in pixels/distance relating pixels of the image when the sample was illuminated with DarkRedFluorescenceExcitationWavelength to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    BlueWhiteScreenImageFile

    An image file containing the image of the sample while using a white light source light and a ___ Nanometer absorbance filter.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    BlueWhiteScreenFilter Wavelength

    The wavelength of light that is filtered out before the source white light hits the sample colonies.
    Format: Single
    Class: Real
    Unit: 1 nanometer
    Programmatic Pattern: GreaterP[0 Nanometer]

    BlueWhiteScreenExposureTime

    The length of time for which the sample was exposed to the BlueWhiteScreen channel while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    BlueWhiteScreenIllumination Direction

    The direction from which the sample was illuminated with white light.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Bottom

    BlueWhiteScreenImageScale

    The scale in pixels/distance relating pixels of the blue white screen image to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

    DarkfieldImageFile

    An image file containing the image of the sample while the sample was illuminated with white light from a ring around the sample.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    DarkfieldExposureTime

    The length of time for which the sample was exposed to Darkfield lighting while taking the image.
    Format: Single
    Class: Real
    Unit: 1 millisecond
    Programmatic Pattern: GreaterP[0 Millisecond]

    DarkfieldIllumination Direction

    The direction from which the sample was illuminated while using Darkfield imaging.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: Side

    DarkfieldScreenImageScale

    The scale in pixels/distance relating pixels of the darkfield image to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

Data Processing

    DataType

    Indicates if this data contains images of the ruler used as a scale reference or analyte images of the sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: AppearanceTypeP

    RulerData

    A relation to ruler data used as a scale reference for this analyte sample.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, Appearance][AnalyteData]

    AnalyteData

    A list of all sample images that use this ruler data as a scale refrence.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data, Appearance][RulerData]

    ImageFile

    A cropped image file containing only the sample.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    UncroppedImageFile

    A raw and unprocessed image file of the sample.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[EmeraldCloudFile]

    Scale

    The scale in pixels/distance relating pixels of the image to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter

Experimental Results

    Image

    Returns the cropped image of the sample.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[UncroppedImageFile]}, ImportCloudFile[Field[UncroppedImageFile]]]

Analysis & Reports

    ColonyAnalysis

    A running log of all colony analyses performed on the image files in order to count and categorize the colonies.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    CellTypes

    The identity models of cell lines that the sample contains as defined by the most recent ColonyAnalysis.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Model[Cell]
Last modified on Mon 26 Sep 2022 15:43:44
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.