Object[Data, CapillaryGelElectrophoresisSDS]
Analytical data collected by a Capillary gel Electrophoresis-Sodium Dodecyl Sulfate (CESDS) experiment. Raw data represents relative migration times (RMT) of analytes in samples, compared to an internal standard.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, CapillaryGelElectrophoresisSDS, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, CapillaryGelElectrophoresisSDS]
Pattern Description: Object[Data, CapillaryGelElectrophoresisSDS]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Replicates
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Replicates]
Ladders
Ladder data acquired under the same experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data]
Standards
Standard data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data]
Blanks
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data]
General
Protocol
The protocol that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Data] | Object[Protocol][EnvironmentalData] | Object[Protocol, AbsorbanceQuantification][VolumeData] | Object[Protocol, AbsorbanceQuantification][EmptyPlateAbsorbance] | Object[Protocol, AbsorbanceQuantification][BufferAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][BlankAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][InitialPurgePressure] | Object[Protocol, AbsorbanceSpectroscopy][PurgePressureLog] | Object[Protocol, AbsorbanceIntensity][BlankAbsorbance] | Object[Protocol, AbsorbanceIntensity][InitialPurgePressure] | Object[Protocol, AbsorbanceIntensity][PurgePressureLog] | Object[Protocol, AbsorbanceKinetics][BlankAbsorbance] | Object[Protocol, AcousticLiquidHandling][Data] | Object[Protocol, AlphaScreen][GainOptimizationData] | Object[Protocol, AlphaScreen][FocalHeightOptimizationData] | Object[Protocol, BioLayerInterferometry][Data] | Object[Protocol, CyclicVoltammetry][ElectrodePretreatmentData] | Object[Protocol, CyclicVoltammetry][CyclicVoltammetryData] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionData] | Object[Protocol, CyclicVoltammetry][PretreatmentSpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][SpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionSpargingPressureLogs] | Object[Protocol, CircularDichroism][BlankData] | Object[Protocol, CircularDichroism][EmptyData] | Object[Protocol, DNASynthesis][DeblockSolutionWeightData] | Object[Protocol, DNASynthesis][WashSolutionWeightData] | Object[Protocol, DNASynthesis][InitialPurgePressure] | Object[Protocol, DynamicFoamAnalysis][Data] | Object[Protocol, Evaporate][CurrentWasteWeightData] | Object[Protocol, Evaporate][WasteWeightData] | Object[Protocol, Evaporate][BathActualTemperature] | Object[Protocol, Evaporate][BucketWeights] | Object[Protocol, PAGE][LadderData] | Object[Protocol, AgaroseGelElectrophoresis][LadderData] | Object[Protocol, MassSpectrometry][MatrixData] | Object[Protocol, MassSpectrometry][CalibrantData] | Object[Protocol, MassSpectrometry][SystemPrimeData] | Object[Protocol, MassSpectrometry][SystemFlushData] | Object[Protocol, HPLC][StandardData] | Object[Protocol, HPLC][BlankData] | Object[Protocol, HPLC][FlushData] | Object[Protocol, HPLC][PrimeData] | Object[Protocol, HPLC][SystemFlushData] | Object[Protocol, HPLC][SystemPrimeData] | Object[Protocol, HPLC][WasteWeightData] | Object[Protocol, FPLC][StandardData] | Object[Protocol, FPLC][BlankData] | Object[Protocol, FPLC][WasteWeightData] | Object[Protocol, FPLC][PrimeData] | Object[Protocol, FPLC][FlushData] | Object[Protocol, FPLC][SystemPrimeData] | Object[Protocol, FPLC][SystemFlushData] | Object[Protocol, GasChromatography][StandardData] | Object[Protocol, GasChromatography][BlankData] | Object[Protocol, GasChromatography][InitialHeliumPressure] | Object[Protocol, GasChromatography][InitialMethanePressure] | Object[Protocol, GasChromatography][FinalHeliumPressure] | Object[Protocol, GasChromatography][FinalMethanePressure] | Object[Protocol, GasChromatography][InitialHydrogenGeneratorWaterWeight] | Object[Protocol, GasChromatography][FinalHydrogenGeneratorWaterWeight] | Object[Protocol, IonChromatography][StandardData] | Object[Protocol, IonChromatography][AnionStandardData] | Object[Protocol, IonChromatography][CationStandardData] | Object[Protocol, IonChromatography][BlankData] | Object[Protocol, IonChromatography][AnionBlankData] | Object[Protocol, IonChromatography][CationBlankData] | Object[Protocol, IonChromatography][FlushData] | Object[Protocol, IonChromatography][AnionFlushData] | Object[Protocol, IonChromatography][CationFlushData] | Object[Protocol, IonChromatography][PrimeData] | Object[Protocol, IonChromatography][AnionPrimeData] | Object[Protocol, IonChromatography][CationPrimeData] | Object[Protocol, IonChromatography][SystemFlushData] | Object[Protocol, IonChromatography][AnionSystemFlushData] | Object[Protocol, IonChromatography][CationSystemFlushData] | Object[Protocol, IonChromatography][SystemPrimeData] | Object[Protocol, IonChromatography][AnionSystemPrimeData] | Object[Protocol, IonChromatography][CationSystemPrimeData] | Object[Protocol, IonChromatography][WasteWeightData] | Object[Protocol, ImageSample][RulerImageData] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, PNASynthesis][WashSolutionWeightData] | Object[Protocol, PNASynthesis][PrimaryWasteWeightData] | Object[Protocol, PNASynthesis][SecondaryWasteWeightData] | Object[Protocol, PNASynthesis][PurgePressureData] | Object[Protocol, PNASynthesis][PurgePressureLog] | Object[Protocol, PNASynthesis][InitialPurgePressure] | Object[Protocol, PeptideSynthesis][WashSolutionWeightData] | Object[Protocol, PeptideSynthesis][PrimaryWasteWeightData] | Object[Protocol, PeptideSynthesis][SecondaryWasteWeightData] | Object[Protocol, PeptideSynthesis][PurgePressureData] | Object[Protocol, PeptideSynthesis][PurgePressureLog] | Object[Protocol, PeptideSynthesis][InitialPurgePressure] | Object[Protocol, Centrifuge][ContainerWeights] | Object[Protocol, Centrifuge][TareWeight] | Object[Protocol, Centrifuge][CollectionContainerWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerTareWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerWeights] | Object[Protocol, IncubateOld][CurrentTemperature] | Object[Protocol, Incubate][ThawActualTemperature] | Object[Protocol, Incubate][SonicationTemperatureData] | Object[Protocol, IRSpectroscopy][BlankData] | Object[Protocol, LCMS][StandardData] | Object[Protocol, LCMS][BlankData] | Object[Protocol, LCMS][PrimeData] | Object[Protocol, LCMS][FlushData] | Object[Protocol, LCMS][SystemPrimeData] | Object[Protocol, LCMS][SystemFlushData] | Object[Protocol, LCMS][CalibrationData] | Object[Protocol, MicrowaveDigestion][ReactionTemperature] | Object[Protocol, MicrowaveDigestion][ReactionPressure] | Object[Protocol, VacuumEvaporation][BucketWeights] | Object[Protocol, VacuumEvaporation][WasteWeightData] | Object[Protocol, SolidPhaseExtraction][PurgePressureLog] | Object[Protocol, SolidPhaseExtraction][InitialPurgePressure] | Object[Protocol, SolidPhaseExtraction][WasteWeightData] | Object[Protocol, SupercriticalFluidChromatography][StandardData] | Object[Protocol, SupercriticalFluidChromatography][BlankData] | Object[Protocol, SupercriticalFluidChromatography][PrimeData] | Object[Protocol, SupercriticalFluidChromatography][FlushData] | Object[Protocol, SupercriticalFluidChromatography][SystemPrimeData] | Object[Protocol, SupercriticalFluidChromatography][SystemFlushData] | Object[Protocol, SupercriticalFluidChromatography][WasteWeightData] | Object[Protocol, Transfer][TareWeights] | Object[Protocol][NitrogenPressureLog] | Object[Protocol][InitialNitrogenPressure] | Object[Protocol][CO2PressureLog] | Object[Protocol][InitialCO2Pressure] | Object[Protocol][ArgonPressureLog] | Object[Protocol][InitialArgonPressure] | Object[Protocol][WasteWeightTare] | Object[Protocol][SecondaryWasteWeightTare] | Object[Protocol][WasteWeight] | Object[Protocol][SecondaryWasteWeight] | Object[Protocol, UVMelting][InitialPurgePressure] | Object[Protocol, UVMelting][PurgePressureLog] | Object[Protocol, UVMelting][AbsorbanceData] | Object[Protocol, Filter][WasteWeightData] | Object[Protocol, MeasureDensity][TareWeights] | Object[Protocol, MeasureDensity][SampleWeights] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, MeasureWeight][ScoutData] | Object[Protocol, MeasureWeight][TareData] | Object[Protocol, MeasureWeight][ResidueWeightData] | Object[Protocol, MeasureWeight][TareWeights] | Object[Protocol, qPCR][StandardData] | Object[Protocol, qPCR][BlankData] | Object[Protocol, CapillaryELISA][StandardData] | Object[Protocol, ELISA][StandardData] | Object[Protocol, ELISA][BlankData] | Object[Protocol, ELISA][PrereadData] | Object[Protocol, RNASynthesis][DeblockSolutionWeightData] | Object[Protocol, RNASynthesis][WashSolutionWeightData] | Object[Protocol, RNASynthesis][InitialPurgePressure] | Object[Protocol, MeasureOsmolality][ControlData] | Object[Protocol, MeasureOsmolality][VoidControlData] | Object[Protocol, MeasureOsmolality][PostRunInstrumentContaminationData] | Object[Protocol, Degas][HeliumPressureLog] | Object[Protocol, Degas][ChannelAGasPressure] | Object[Protocol, Degas][ChannelBGasPressure] | Object[Protocol, Degas][ChannelCGasPressure] | Object[Protocol, Degas][ChannelDGasPressure] | Object[Protocol, Degas][VacuumSensorPressure] | Object[Protocol, CapillaryGelElectrophoresisSDS][InjectionTable, Data] | Object[Protocol, CapillaryIsoelectricFocusing][InjectionTable, Data] | Object[Protocol, Nephelometry][BlankMeasurement] | Object[Protocol, NephelometryKinetics][BlankMeasurement] | Object[Protocol, MeasureDensity][AirCheckData] | Object[Protocol, MeasureDensity][WaterCheckData] | Object[Protocol, MeasureRefractiveIndex][Data]
Qualifications
The Qualifications that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification][Data] | Object[Qualification][WasteWeightTare] | Object[Qualification][SecondaryWasteWeightTare] | Object[Qualification][WasteWeight] | Object[Qualification][SecondaryWasteWeight] | Object[Qualification][EnvironmentalData] | Object[Qualification, CalibrateDNASynthesizer][InitialPurgePressure] | Object[Qualification, CalibrateDNASynthesizer][CalibrationWeightData] | Object[Qualification, EngineBenchmark][SelectedSensorData] | Object[Qualification, EngineBenchmark][RecordedSensorData] | Object[Qualification, ELISA][AbsorbanceQualificationData]
Maintenance
The maintenance that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Maintenance][Data] | Object[Maintenance, CalibrateVolume][EmptyDistances] | Object[Maintenance, CalibrateVolume][TareDistance] | Object[Maintenance, CalibratePathLength][EmptyDistances] | Object[Maintenance, CalibratePathLength][StandardDistances] | Object[Maintenance, CalibratePathLength][EmptyAbsorbance] | Object[Maintenance][InitialNitrogenPressure] | Object[Maintenance][NitrogenPressureLog] | Object[Maintenance][InitialCO2Pressure] | Object[Maintenance][CO2PressureLog] | Object[Maintenance][InitialArgonPressure] | Object[Maintenance][ArgonPressureLog] | Object[Maintenance][EnvironmentalData] | Object[Maintenance][WasteWeightTare] | Object[Maintenance][SecondaryWasteWeightTare] | Object[Maintenance][WasteWeight] | Object[Maintenance][SecondaryWasteWeight] | Object[Maintenance, Flush][SystemFlushData]
Instrument
Instrument this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Sensor
Sensor this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sensor][Data]
DataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
AliquotSamples
Aliquot samples that were directly assayed to generate this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
SamplesIn
Samples that were assayed to generate this data. Please note that if the samples were aliquoted, this field will contain the input samples.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SamplesOut
Samples produced in the course of generating this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
Cartridge
The consumable inserted onto the instrument and sequentially loaded with samples for separation and analysis. The cartridge holds a capillary and the anode running buffer.
Format: Single
Class: Link
Programmatic Pattern: _Link
SampleTemperature
The sample tray temperature at which samples are maintained while awaiting injection.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
InjectionIndex
The numeric position indicating when this data was measured for the experiment with respect to the other column primes, sample injections, standard injections, blank injections, and column flushes (e.g. 1 is the first measurement run).
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
SampleType
Indicates whether these data represent a ladder, a blank, a standard, or an unknown sample measurement.
Format: Single
Class: Expression
Programmatic Pattern: Sample | Ladder | Standard | Blank
Well
The well in the plate from which the data were collected.
Format: Single
Class: Expression
Programmatic Pattern: WellP
SeparationMatrix
The sieving matrix loaded onto the capillary before each sample for separation. The mash-like matrix slows the migration of proteins based on their size so that larger proteins migrate slower than smaller ones.
Format: Single
Class: Link
Programmatic Pattern: _Link
TotalVolume
Indicates the final volume in the assay tube prior to loading onto AssayContainer. Each tube contains a Sample, an InternalStandard, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SampleVolume
Indicates the sample volume in the assay tube prior to loading onto AssayContainer. Each tube contains a Sample, an InternalStandard, an SDSBuffer, and a ReducingAgent and/or an AlkylatingAgent.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PremadeMasterMixReagent
Indicates the reagent with all required components in sample preparation for capillary Gel Electrophoresis SDS. Premade mastermix contains an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Format: Single
Class: Link
Programmatic Pattern: _Link
PremadeMasterMixVolume
Indicates the volume of Premade MasterMix added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
PremadeMasterMixDiluent
Indicates the solution used to dilute the premade mastermix in sample preparation for capillary Gel Electrophoresis SDS. Premade mastermix contains an SDS buffer, an internal standard, and reducing and / or alkylating agents (if applicable).
Format: Single
Class: Link
Programmatic Pattern: _Link
Diluent
Indicates the solution used to dilute components in the mastermix to their working concentration.
Format: Single
Class: Link
Programmatic Pattern: _Link
InternalReference
Indicates the internal standard stock solution added in sample preparation. The internal standard stock solution contains the analyte that serves as the reference by which Relative Migration Time is normalized. By default a 10 KDa marker is used.
Format: Single
Class: Link
Programmatic Pattern: _Link
InternalReferenceVolume
Indicates the volume of the internal standard stock solution added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ConcentratedSDSBuffer
Indicates the SDS concentrated solution required for sample preparation. SDS denatures proteins and imparts an equal charge-to-mass ratio to all proteins, facilitating separation exclusively based on size.
Format: Single
Class: Link
Programmatic Pattern: _Link
ConcentratedSDSBufferVolume
Indicates the volume of the SDSBuffer concentrated solution added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
SDSBuffer
Indicates the SDS solution required for sample preparation. SDS denatures proteins and imparts an equal charge-to-mass ratio to all proteins, facilitating separation exclusively based on size.
Format: Single
Class: Link
Programmatic Pattern: _Link
SDSBufferVolume
Indicates the volume of the SDSBuffer solution added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
ReducingAgent
Indicates the chemical stock solution added in sample preparation to break disulfide bridges in proteins.
Format: Single
Class: Link
Programmatic Pattern: _Link
ReducingAgentVolume
Indicates the volume of the ReducingAgent stock solution added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
AlkylatingAgent
Indicates the AlkylatingAgent stock solution added in sample preparation. Alkylation of free cysteines can reduce fragmentation and increase reproducibility in CESDS.
Format: Single
Class: Link
Programmatic Pattern: _Link
AlkylatingAgentVolume
Indicates the volume of the AlkylatingAgent stock solution added in TotalVolume sample preparation.
Format: Single
Class: Real
Unit: 1 microliter
Programmatic Pattern: GreaterEqualP[0 Microliter]
DenaturingTemperature
Indicates the temperature at which proteins are denatured.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0 Kelvin]
DenaturingTime
Indicates the duration of incubation at DenaturingTemperature.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0 Second]
CoolingTemperature
Indicates the temperature at which proteins are held after denaturation.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0 Kelvin]
CoolingTime
Indicates the duration of incubation at CoolingTemperature.
Format: Single
Class: Real
Programmatic Pattern: GreaterP[0 Second]
InjectionVoltageDuration Profile
Indicates the series of voltages and durations applied onto the capillary while docked in the sample to electroinject proteins in the sample into the matrix in the capillary.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
SeparationVoltageDuration Profile
Indicates the series of voltages and durations applied onto the capillary while docked in running buffer to seperate proteins by molecular weight as they migrate through the separation matrix in the capillary.
Format: Single
Class: Expression
Programmatic Pattern: {{GreaterEqualP[0 Volt], GreaterP[0 Second]}..}
LadderMolecularWeights
Markers included in ladder and their moleclular weight or identification.
Format: Multiple
[[1]] Marker
Header: Marker
Class: Expression
Programmatic Pattern: _String|_Link
[[2]] Molecular Weight / ID
Header: Molecular Weight / ID
Class: Expression
Programmatic Pattern: GreaterP[0 Kilodalton]|_String
Experimental Results
UVAbsorbanceData
The electropherogram of UV Absorbance (A280) vs. time.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Second, AbsorbanceUnit}]
ProcessedUVAbsorbanceData
The electropherogram of UV Absorbance (A280) vs. time as processed by the Compass for iCE software.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Second, AbsorbanceUnit}]
UVAbsorbanceBackgroundData
The electropherogram of UV Absorbance background vs. time.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 AbsorbanceUnit']
Programmatic Pattern: QuantityCoordinatesP[{Second, AbsorbanceUnit}]
CurrentData
The Current vs. time curve of injection and separation.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 milliampere']
Programmatic Pattern: QuantityCoordinatesP[{Second, Milliampere}]
VoltageData
The Voltage vs. time curve of injection and separation.
Format: Single
Class: QuantityArray
Unit: ['1 second', '1 volt']
Programmatic Pattern: QuantityCoordinatesP[{Second, Volt}]
Data Processing
RelativeMigrationData
The electropherogram of UV Absorbance (A280) vs. relative migration time to the InternalStandard. This field is generated by the most recent peak-picking in PeaksAnalyses.
Format: Single
Class: Compressed
Programmatic Pattern: CoordinatesP
Analysis & Reports
LadderAnalyses
The molecular weight marker analyses performed on Relative Migration Time data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
SmoothingAnalyses
Smoothing analysis performed on Relative Migration Time data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
PeaksAnalyses
Peak picking analyses performed on ProcessedUVAbsorbanceData.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
RelativeMigrationPeaks Analyses
Peak picking analyses performed on RelativeMigrationData.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
FitAnalyses
Fit analyses performed on Relative Migration Time data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
Last modified on Mon 26 Sep 2022 15:43:44