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Object[Data, DNASequencing]

DNA sequencing measures the fluorescent wavelength and intensity emitted from a labeled nucleotide and assigns the identity of the nucleotide.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DNASequencing, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, DNASequencing]
    Pattern Description: Object[Data, DNASequencing]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Experimental Results

    SequencingElectropherogram Channel1

    The raw electropherogram data for one channel obtained by the instrument in terms of the intensity of the fluorescent signal over time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 ArbitraryUnits', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{ArbitraryUnit, ArbitraryUnit}]

    SequencingElectropherogram Channel2

    The raw electropherogram data for one channel obtained by the instrument in terms of the intensity of the fluorescent signal over time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 ArbitraryUnits', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{ArbitraryUnit, ArbitraryUnit}]

    SequencingElectropherogram Channel3

    The raw electropherogram data for one channel obtained by the instrument in terms of the intensity of the fluorescent signal over time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 ArbitraryUnits', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{ArbitraryUnit, ArbitraryUnit}]

    SequencingElectropherogram Channel4

    The raw electropherogram data for one channel obtained by the instrument in terms of the intensity of the fluorescent signal over time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 ArbitraryUnits', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityCoordinatesP[{ArbitraryUnit, ArbitraryUnit}]

Data Processing

    Channel1BaseAssignment

    The assignment of the nucleotide base for one channel of raw electropheogram data.
    Format: Single
    Class: String
    Programmatic Pattern: A | T | G | C | Unknown

    Channel2BaseAssignment

    The assignment of the nucleotide base for one channel of raw electropheogram data.
    Format: Single
    Class: String
    Programmatic Pattern: A | T | G | C | Unknown

    Channel3BaseAssignment

    The assignment of the nucleotide base for one channel of raw electropheogram data.
    Format: Single
    Class: String
    Programmatic Pattern: A | T | G | C | Unknown

    Channel4BaseAssignment

    The assignment of the nucleotide base for one channel of raw electropheogram data.
    Format: Single
    Class: String
    Programmatic Pattern: A | T | G | C | Unknown

Analysis & Reports

    SequenceAnalyses

    A list of base-calling analyses conducted on the fluorescence intensity data from the raw electropherogram.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    SequencingAssignments

    A list of base pair assignments from analyses conducted on the fluorescence intensity data from the raw electropherogram.
    Format: Multiple
    Class: String
    Programmatic Pattern: _String

    QualityValues

    A list of the quality values of the base assignments analyses indicating the accuracy of the base assignment. A quality value of >20 is considered an accurate base assignment; a quality value between 15-19 is considered a mediocre accuracy; a quality value below 15 is considered poor accuracy.
    Format: Multiple
    Class: Integer
    Programmatic Pattern: _Integer
Last modified on Mon 26 Sep 2022 15:43:44
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