ecl-logo Documentation

Object[Data, FluorescenceIntensity]

Measurements of intensity of a fluorescence emission at a fixed wavelength.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescenceIntensity, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescenceIntensity]
    Pattern Description: Object[Data, FluorescenceIntensity]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Fluorescence Measurement

    ReadLocation

    The side of the microplate from which the readings were taken.
    Format: Single
    Class: Expression
    Programmatic Pattern: ReadLocationP

    DelayTime

    The amount of time allowed to pass after excitation before fluorescence measurement occurred.
    Format: Single
    Class: Real
    Unit: 1 microsecond
    Programmatic Pattern: GreaterP[0 Microsecond]

    ReadTime

    The amount of time for which the fluorescence measurement reading occurred.
    Format: Single
    Class: Real
    Unit: 1 microsecond
    Programmatic Pattern: GreaterP[0 Microsecond]

Experimental Results

    Intensities

    For each member of EmissionWavelengths, the measured fluorescence intensity value.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: UnitsP[RFU]

    DualEmissionIntensities

    For each member of DualEmissionWavelengths, the measured fluorescence intensity value.
    Format: Multiple
    Class: Real
    Unit: 1 Rfus
    Programmatic Pattern: UnitsP[RFU]

    Temperature

    Temperature inside the sample chamber at the time of acquisition.
    Format: Single
    Class: Real
    Unit: 1 degree Celsius
    Programmatic Pattern: GreaterP[0 Kelvin]

    DetectorRangeExceeded

    Indicates if any of the recorded intensities maxed out the instrument's detector.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    InvalidValuesExcluded

    Indicates if any of the recorded intensities are below 0 RFU and have been deleted from the final result.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP
Last modified on Mon 26 Sep 2022 15:43:44
EMERALD CLOUD LAB
15500 Wells Port Drive
Austin, TX 78728
© 2023 Emerald Cloud Lab, Inc.