ecl-logo Documentation

Object[Data, FluorescenceKinetics]

Fluorescence measurements of a sample monitored over a period of time to study reaction trajectories.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, FluorescenceKinetics, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

General

Fluorescence Measurement

    ReadLocation

    The side of the plate from which the readings were taken.
    Format: Single
    Class: Expression
    Programmatic Pattern: ReadLocationP

    DelayTime

    The amount of time allowed to pass after excitation before fluorescence measurement occurred.
    Format: Single
    Class: Real
    Unit: 1 microsecond
    Programmatic Pattern: GreaterP[0 Microsecond]

    ReadTime

    The amount of time for which the fluorescence measurement reading occurred.
    Format: Single
    Class: Real
    Unit: 1 microsecond
    Programmatic Pattern: GreaterP[0 Microsecond]

Analysis & Reports

    RateFittingAnalyses

    The data objects used to create a standard curve relating fluorescence to concentration for this object.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Experimental Results

    EmissionTrajectories

    Emission fluorescence readings taken as a function of time, for the configuration of excitation wavelength, emission wavelength and sensitivity.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Second, RFU}]

    DualEmissionTrajectories

    Emission fluorescence readings taken as a function of time, for the configuration of excitation wavelength, secondary emission wavelength and secondary sensitivity.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 second', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Second, RFU}]

    Temperature

    Temperature readings taken in the sample chamber, for the trajectories recorded.
    Format: Single
    Class: QuantityArray
    Unit: ['1 second', '1 degree Celsius']
    Programmatic Pattern: QuantityCoordinatesP[{Second, Celsius}]

    DetectorRangeExceeded

    Indicates if any of the recorded intensities maxed out the instrument's detector.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP

    InvalidValuesExcluded

    Indicates if any of the recorded intensities are below 0 RFU and have been deleted from the final result.
    Format: Single
    Class: Boolean
    Programmatic Pattern: BooleanP
Last modified on Mon 26 Sep 2022 15:43:44