Object[Data, MeltingCurve]
Measurements of absorbance, fluorescence, static light scattering, and/or dynamic light scattering at specific wavelengths with varying temperatures.
Organizational Information
Name
Name of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: A string.
ID
ID of this Object.
Format: Single
Class: String
Programmatic Pattern: _String
Pattern Description: The ID of this object.
Object
Object of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, MeltingCurve, _String]
Pattern Description: The object reference of this object.
Type
Type of this Object.
Format: Single
Class: Expression
Programmatic Pattern: Object[Data, MeltingCurve]
Pattern Description: Object[Data, MeltingCurve]
Notebook
Notebook this object belongs to.
Format: Single
Class: Link
Programmatic Pattern: _Link
Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
Relation: Object[LaboratoryNotebook][Objects]
Replicates
Data acquired under replicated experimental conditions to this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Data][Replicates]
General
Protocol
The protocol that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Protocol][Data] | Object[Protocol][EnvironmentalData] | Object[Protocol, AbsorbanceQuantification][VolumeData] | Object[Protocol, AbsorbanceQuantification][EmptyPlateAbsorbance] | Object[Protocol, AbsorbanceQuantification][BufferAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][BlankAbsorbance] | Object[Protocol, AbsorbanceSpectroscopy][InitialPurgePressure] | Object[Protocol, AbsorbanceSpectroscopy][PurgePressureLog] | Object[Protocol, AbsorbanceIntensity][BlankAbsorbance] | Object[Protocol, AbsorbanceIntensity][InitialPurgePressure] | Object[Protocol, AbsorbanceIntensity][PurgePressureLog] | Object[Protocol, AbsorbanceKinetics][BlankAbsorbance] | Object[Protocol, AcousticLiquidHandling][Data] | Object[Protocol, AlphaScreen][GainOptimizationData] | Object[Protocol, AlphaScreen][FocalHeightOptimizationData] | Object[Protocol, BioLayerInterferometry][Data] | Object[Protocol, CyclicVoltammetry][ElectrodePretreatmentData] | Object[Protocol, CyclicVoltammetry][CyclicVoltammetryData] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionData] | Object[Protocol, CyclicVoltammetry][PretreatmentSpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][SpargingPressureLogs] | Object[Protocol, CyclicVoltammetry][PostMeasurementStandardAdditionSpargingPressureLogs] | Object[Protocol, CircularDichroism][BlankData] | Object[Protocol, CircularDichroism][EmptyData] | Object[Protocol, DNASynthesis][DeblockSolutionWeightData] | Object[Protocol, DNASynthesis][WashSolutionWeightData] | Object[Protocol, DNASynthesis][InitialPurgePressure] | Object[Protocol, DynamicFoamAnalysis][Data] | Object[Protocol, Evaporate][CurrentWasteWeightData] | Object[Protocol, Evaporate][WasteWeightData] | Object[Protocol, Evaporate][BathActualTemperature] | Object[Protocol, Evaporate][BucketWeights] | Object[Protocol, PAGE][LadderData] | Object[Protocol, AgaroseGelElectrophoresis][LadderData] | Object[Protocol, MassSpectrometry][MatrixData] | Object[Protocol, MassSpectrometry][CalibrantData] | Object[Protocol, MassSpectrometry][SystemPrimeData] | Object[Protocol, MassSpectrometry][SystemFlushData] | Object[Protocol, HPLC][StandardData] | Object[Protocol, HPLC][BlankData] | Object[Protocol, HPLC][FlushData] | Object[Protocol, HPLC][PrimeData] | Object[Protocol, HPLC][SystemFlushData] | Object[Protocol, HPLC][SystemPrimeData] | Object[Protocol, HPLC][WasteWeightData] | Object[Protocol, FPLC][StandardData] | Object[Protocol, FPLC][BlankData] | Object[Protocol, FPLC][WasteWeightData] | Object[Protocol, FPLC][PrimeData] | Object[Protocol, FPLC][FlushData] | Object[Protocol, FPLC][SystemPrimeData] | Object[Protocol, FPLC][SystemFlushData] | Object[Protocol, GasChromatography][StandardData] | Object[Protocol, GasChromatography][BlankData] | Object[Protocol, GasChromatography][InitialHeliumPressure] | Object[Protocol, GasChromatography][InitialMethanePressure] | Object[Protocol, GasChromatography][FinalHeliumPressure] | Object[Protocol, GasChromatography][FinalMethanePressure] | Object[Protocol, GasChromatography][InitialHydrogenGeneratorWaterWeight] | Object[Protocol, GasChromatography][FinalHydrogenGeneratorWaterWeight] | Object[Protocol, IonChromatography][StandardData] | Object[Protocol, IonChromatography][AnionStandardData] | Object[Protocol, IonChromatography][CationStandardData] | Object[Protocol, IonChromatography][BlankData] | Object[Protocol, IonChromatography][AnionBlankData] | Object[Protocol, IonChromatography][CationBlankData] | Object[Protocol, IonChromatography][FlushData] | Object[Protocol, IonChromatography][AnionFlushData] | Object[Protocol, IonChromatography][CationFlushData] | Object[Protocol, IonChromatography][PrimeData] | Object[Protocol, IonChromatography][AnionPrimeData] | Object[Protocol, IonChromatography][CationPrimeData] | Object[Protocol, IonChromatography][SystemFlushData] | Object[Protocol, IonChromatography][AnionSystemFlushData] | Object[Protocol, IonChromatography][CationSystemFlushData] | Object[Protocol, IonChromatography][SystemPrimeData] | Object[Protocol, IonChromatography][AnionSystemPrimeData] | Object[Protocol, IonChromatography][CationSystemPrimeData] | Object[Protocol, IonChromatography][WasteWeightData] | Object[Protocol, ImageSample][RulerImageData] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, PNASynthesis][WashSolutionWeightData] | Object[Protocol, PNASynthesis][PrimaryWasteWeightData] | Object[Protocol, PNASynthesis][SecondaryWasteWeightData] | Object[Protocol, PNASynthesis][PurgePressureData] | Object[Protocol, PNASynthesis][PurgePressureLog] | Object[Protocol, PNASynthesis][InitialPurgePressure] | Object[Protocol, PeptideSynthesis][WashSolutionWeightData] | Object[Protocol, PeptideSynthesis][PrimaryWasteWeightData] | Object[Protocol, PeptideSynthesis][SecondaryWasteWeightData] | Object[Protocol, PeptideSynthesis][PurgePressureData] | Object[Protocol, PeptideSynthesis][PurgePressureLog] | Object[Protocol, PeptideSynthesis][InitialPurgePressure] | Object[Protocol, Centrifuge][ContainerWeights] | Object[Protocol, Centrifuge][TareWeight] | Object[Protocol, Centrifuge][CollectionContainerWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerTareWeights] | Object[Protocol, Centrifuge][CounterbalanceContainerWeights] | Object[Protocol, IncubateOld][CurrentTemperature] | Object[Protocol, Incubate][ThawActualTemperature] | Object[Protocol, Incubate][SonicationTemperatureData] | Object[Protocol, IRSpectroscopy][BlankData] | Object[Protocol, LCMS][StandardData] | Object[Protocol, LCMS][BlankData] | Object[Protocol, LCMS][PrimeData] | Object[Protocol, LCMS][FlushData] | Object[Protocol, LCMS][SystemPrimeData] | Object[Protocol, LCMS][SystemFlushData] | Object[Protocol, LCMS][CalibrationData] | Object[Protocol, MicrowaveDigestion][ReactionTemperature] | Object[Protocol, MicrowaveDigestion][ReactionPressure] | Object[Protocol, VacuumEvaporation][BucketWeights] | Object[Protocol, VacuumEvaporation][WasteWeightData] | Object[Protocol, SolidPhaseExtraction][PurgePressureLog] | Object[Protocol, SolidPhaseExtraction][InitialPurgePressure] | Object[Protocol, SolidPhaseExtraction][WasteWeightData] | Object[Protocol, SupercriticalFluidChromatography][StandardData] | Object[Protocol, SupercriticalFluidChromatography][BlankData] | Object[Protocol, SupercriticalFluidChromatography][PrimeData] | Object[Protocol, SupercriticalFluidChromatography][FlushData] | Object[Protocol, SupercriticalFluidChromatography][SystemPrimeData] | Object[Protocol, SupercriticalFluidChromatography][SystemFlushData] | Object[Protocol, SupercriticalFluidChromatography][WasteWeightData] | Object[Protocol, Transfer][TareWeights] | Object[Protocol][NitrogenPressureLog] | Object[Protocol][InitialNitrogenPressure] | Object[Protocol][CO2PressureLog] | Object[Protocol][InitialCO2Pressure] | Object[Protocol][ArgonPressureLog] | Object[Protocol][InitialArgonPressure] | Object[Protocol][WasteWeightTare] | Object[Protocol][SecondaryWasteWeightTare] | Object[Protocol][WasteWeight] | Object[Protocol][SecondaryWasteWeight] | Object[Protocol, UVMelting][InitialPurgePressure] | Object[Protocol, UVMelting][PurgePressureLog] | Object[Protocol, UVMelting][AbsorbanceData] | Object[Protocol, Filter][WasteWeightData] | Object[Protocol, MeasureDensity][TareWeights] | Object[Protocol, MeasureDensity][SampleWeights] | Object[Protocol, MeasureViscosity][WasteWeightData] | Object[Protocol, MeasureWeight][ScoutData] | Object[Protocol, MeasureWeight][TareData] | Object[Protocol, MeasureWeight][ResidueWeightData] | Object[Protocol, MeasureWeight][TareWeights] | Object[Protocol, qPCR][StandardData] | Object[Protocol, qPCR][BlankData] | Object[Protocol, CapillaryELISA][StandardData] | Object[Protocol, ELISA][StandardData] | Object[Protocol, ELISA][BlankData] | Object[Protocol, ELISA][PrereadData] | Object[Protocol, RNASynthesis][DeblockSolutionWeightData] | Object[Protocol, RNASynthesis][WashSolutionWeightData] | Object[Protocol, RNASynthesis][InitialPurgePressure] | Object[Protocol, MeasureOsmolality][ControlData] | Object[Protocol, MeasureOsmolality][VoidControlData] | Object[Protocol, MeasureOsmolality][PostRunInstrumentContaminationData] | Object[Protocol, Degas][HeliumPressureLog] | Object[Protocol, Degas][ChannelAGasPressure] | Object[Protocol, Degas][ChannelBGasPressure] | Object[Protocol, Degas][ChannelCGasPressure] | Object[Protocol, Degas][ChannelDGasPressure] | Object[Protocol, Degas][VacuumSensorPressure] | Object[Protocol, CapillaryGelElectrophoresisSDS][InjectionTable, Data] | Object[Protocol, CapillaryIsoelectricFocusing][InjectionTable, Data] | Object[Protocol, Nephelometry][BlankMeasurement] | Object[Protocol, NephelometryKinetics][BlankMeasurement] | Object[Protocol, MeasureDensity][AirCheckData] | Object[Protocol, MeasureDensity][WaterCheckData] | Object[Protocol, MeasureRefractiveIndex][Data]
Qualifications
The Qualifications that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Qualification][Data] | Object[Qualification][WasteWeightTare] | Object[Qualification][SecondaryWasteWeightTare] | Object[Qualification][WasteWeight] | Object[Qualification][SecondaryWasteWeight] | Object[Qualification][EnvironmentalData] | Object[Qualification, CalibrateDNASynthesizer][InitialPurgePressure] | Object[Qualification, CalibrateDNASynthesizer][CalibrationWeightData] | Object[Qualification, EngineBenchmark][SelectedSensorData] | Object[Qualification, EngineBenchmark][RecordedSensorData] | Object[Qualification, ELISA][AbsorbanceQualificationData]
Maintenance
The maintenance that generated this data.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Maintenance][Data] | Object[Maintenance, CalibrateVolume][EmptyDistances] | Object[Maintenance, CalibrateVolume][TareDistance] | Object[Maintenance, CalibratePathLength][EmptyDistances] | Object[Maintenance, CalibratePathLength][StandardDistances] | Object[Maintenance, CalibratePathLength][EmptyAbsorbance] | Object[Maintenance][InitialNitrogenPressure] | Object[Maintenance][NitrogenPressureLog] | Object[Maintenance][InitialCO2Pressure] | Object[Maintenance][CO2PressureLog] | Object[Maintenance][InitialArgonPressure] | Object[Maintenance][ArgonPressureLog] | Object[Maintenance][EnvironmentalData] | Object[Maintenance][WasteWeightTare] | Object[Maintenance][SecondaryWasteWeightTare] | Object[Maintenance][WasteWeight] | Object[Maintenance][SecondaryWasteWeight] | Object[Maintenance, Flush][SystemFlushData]
Instrument
Instrument this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Instrument]
Sensor
Sensor this data was obtained using.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sensor][Data]
DataFile
The file containing the raw unprocessed data generated by the instrument.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
AliquotSamples
Aliquot samples that were directly assayed to generate this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
SamplesIn
Samples that were assayed to generate this data. Please note that if the samples were aliquoted, this field will contain the input samples.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
SamplesOut
Samples produced in the course of generating this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Sample][Data]
BufferModel
The model of the buffer used to dilute the sample(s) in the cuvette prior to the absorbance measurement.
Format: Single
Class: Link
Programmatic Pattern: _Link
DetectionReagent
The fluorescent reagent used to detect temperature dependent structural changes in the analyte.
Format: Single
Class: Link
Programmatic Pattern: _Link
PassiveReferenceDye
The fluorescent reagent which does not interact with the analyte and is used to normalize fluorescence emission measurements.
Format: Single
Class: Link
Programmatic Pattern: _Link
Wavelength
The wavelength of the emission monochromator used for filtering the source light.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Meter]
Bandwidth
The width of the light surrounding Wavelength which the monochromator does not filter out.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Meter]
AveragingTime
The length of time for which the diode reading is averaged when detecting emission signal.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterEqualP[0 Second]
CuvetteModel
The model of the cuvette containing the sample(s) and through which the light beam is passed during the absorbance measurement.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Model[Container, Cuvette]
CuvetteNumber
The position of the sample's cuvette inside the heatblock of the spectrophotometer instrument during absorbance measurement.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
ExcitationWavelength
The wavelength of light used to excite the fluorescent detection reagent.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter Nano]
EmissionWavelength
The wavelength of light emitted by the fluorescence detection reagent upon excitation and measured by the fluorescence detector.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter Nano]
MinEmissionWavelength
The minimum wavelength of light emitted by the fluorescence detection reagent upon excitation and measured by the fluorescence detector.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter Nano]
MaxEmissionWavelength
The maximum emission wavelength collected during the experiment.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Meter Nano]
FluorescenceLaserPower
The percentage of full power the 266nm UV laser operated at during measurement.
Format: Single
Class: Expression
Programmatic Pattern: LaserPowerFilterP
StaticLightScatteringLaser Power
The percentage of full power the 473nm Blue laser operated at during measurement.
Format: Single
Class: Expression
Programmatic Pattern: LaserPowerFilterP
Method Information
DilutionCurve
The data acquired for a single analyte at different dilutions.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Well
The well position of this sample in the assay plate during measurement.
Format: Single
Class: String
Programmatic Pattern: WellP
StaticLightScattering Excitation
The wavelength(s) used for static light scattering measurements.
Format: Multiple
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterP[0 Objects`Private`NanoMeter]
CapillaryWell
The capillary well position of this sample during measurement.
Format: Single
Class: String
Programmatic Pattern: WellP
CapillaryStrip
The capillary strip position of this sample during measurement.
Format: Single
Class: Link
Programmatic Pattern: _Link
Relation: Object[Container, Plate, CapillaryStrip]
Optical Information
MinWavelength
The lower limit of the wavelength range over which sample absorbance is read.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
MaxWavelength
The upper limit of the wavelength range over which sample absorbance is read.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nano Meter]
Detection
OptimizeFluorescenceLaser Power
Indicates if the fluorescence laser power filter is adjusted prior to sample measurement.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
OptimizeStaticLightScattering LaserPower
Indicates if the static light scattering laser power filter is matched to the optimal fluorescence laser power prior to sample measurement.
Format: Single
Class: Boolean
Programmatic Pattern: BooleanP
LaserOptimizationEmission WavelengthRange
The wavelength(s) of the sample's spectra used to evaluate optimal laser setting.
Format: Single
Class: Expression
Programmatic Pattern: {GreaterP[0 Nanometer]..}
LaserOptimizationTarget EmissionIntensityRange
The intensity range, expressed as a counts, used to evaluate optimal laser setting.
Format: Single
Class: Expression
Programmatic Pattern: {GreaterP[0]..}
FluorescenceLaserPower OptimizationResult
Indicates if the fluorescence laser power filter optimization was successful. "FailureLowSignal" indicates the laser power could not be optimized because signal intensity at 100% laser power is lower than the LaserOptimizationTargetEmissionIntensityRange. "FailureSignalSaturation" indicates the laser power could not be optimized because signal intensity at 13% laser power is higher than the LaserOptimizationTargetEmissionIntensityRange.
Format: Single
Class: Expression
Programmatic Pattern: LaserOptimizationResultP
Experimental Results
MeltingCurve
The measurement signal as a function of temperature during the first heating cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
CoolingCurve
The measurement signal as a function of temperature during the first cooling cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
SecondaryMeltingCurve
The measurement signal as a function of temperature during the second heating cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
SecondaryCoolingCurve
The measurement signal as a function of temperature during the second cooling cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
TertiaryMeltingCurve
The measurement signal as a function of temperature during the third heating cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
TertiaryCoolingCurve
The measurement signal as a function of temperature during the third cooling cycle If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
QuaternaryMeltingCurve
The measurement signal as a function of temperature during the fourth heating cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
QuaternaryCoolingCurve
The measurement signal as a function of temperature during the fourth cooling cycle If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
QuinaryMeltingCurve
The measurement signal as a function of temperature during the fourth heating cycle. If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
QuinaryCoolingCurve
The measurement signal as a function of temperature during the fourth cooling cycle If MinWavelength and MaxWavelength are specified, the wavelength for this field will be 280 Nanometer if within the range and the sample is a peptide, 260 Nanometer if the sample is not a peptide and this value is within the MinWavelength/MaxWavelength range, or the MinWavelength otherwise.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
MeltingCurve3D
The measurement signal at each wavelength for each temperature point during the first heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
CoolingCurve3D
The measurement signal at each wavelength for each temperature point during the first cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
SecondaryMeltingCurve3D
The measurement signal at each wavelength for each temperature point during the second heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
SecondaryCoolingCurve3D
The measurement signal at each wavelength for each temperature point during the second cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
TertiaryMeltingCurve3D
The measurement signal at each wavelength for each temperature point during the third heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
TertiaryCoolingCurve3D
The measurement signal at each wavelength for each temperature point during the third cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
QuaternaryMeltingCurve3D
The measurement signal at each wavelength for each temperature point during the fourth heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
QuaternaryCoolingCurve3D
The measurement signal at each wavelength for each temperature point during the fourth cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
QuinaryMeltingCurve3D
The measurement signal at each wavelength for each temperature point during the fifth heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
QuinaryCoolingCurve3D
The measurement signal at each wavelength for each temperature point during the fifth cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
AggregationCurve
The recorded light scattering intensity (counts) as a function of increasing temperature during the first heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
AggregationRecoveryCurve
The recorded light scattering intensity (counts) as a function of decreasing temperature during the first cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
SecondaryAggregationCurve
The recorded light scattering intensity (counts) as a function of increasing temperature during the second heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
SecondaryAggregationRecovery Curve
The recorded light scattering intensity (counts) as a function of decreasing temperature during the second cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
TertiaryAggregationCurve
The recorded light scattering intensity (counts) as a function of increasing temperature during the third heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
TertiaryAggregationRecovery Curve
The recorded light scattering intensity (counts) as a function of decreasing temperature during the third cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, ArbitraryUnit}..}]
AggregationCurve3D
The recorded light scattering intensity (counts) at each wavelength as a function of increasing temperature during the first heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
AggregationRecoveryCurve3D
The recorded light scattering intensity (counts) at each wavelength as a function of decreasing temperature during the first cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
SecondaryAggregationCurve3D
The recorded light scattering intensity (counts) at each wavelength as a function of increasing temperature during the second heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
SecondaryAggregationRecovery Curve3D
The recorded light scattering intensity (counts) at each wavelength as a function of decreasing temperature during the second cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
TertiaryAggregationCurve3D
The recorded light scattering intensity (counts) at each wavelength as a function of increasing temperature during the third heating cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
TertiaryAggregationRecovery Curve3D
The recorded light scattering intensity (counts) at each wavelength as a function of decreasing temperature during the third cooling cycle.
Format: Single
Class: QuantityArray
Unit: ['1 degree Celsius', '1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityArrayP[{{Celsius, Nanometer, ArbitraryUnit}..}]
DynamicLightScattering
Indicates if a DLS measurement of each sample is made before and after the thermal ramping.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
DynamicLightScattering Measurements
Indicates if a DLS measurement of each sample is made before thermal ramping, after thermal ramping, or both before and after thermal ramping.
Format: Multiple
Class: Expression
Programmatic Pattern: Before | After
NumberOfDynamicLight ScatteringAcquisitions
For each DLS measurement, the number of series of speckle patterns that are each collected over the AcquisitionTime to create the measurement's autocorrelation curve.
Format: Single
Class: Integer
Programmatic Pattern: GreaterP[0, 1]
DynamicLightScattering AcquisitionTime
For each DLS measurement, the length of time that each acquisition generates speckle patterns to create the measurement's autocorrelation curve.
Format: Single
Class: Real
Unit: 1 second
Programmatic Pattern: GreaterP[0 Second]
AutomaticDynamicLight ScatteringLaserSettings
Indicates if the DynamicLightScatteringLaserPower and DynamicLightScatteringDiodeAttenuation are automatically set at the beginning of the assay by the Instrument to levels ideal for the samples.
Format: Single
Class: Expression
Programmatic Pattern: BooleanP
DynamicLightScatteringLaser Power
The percent of the max dynamic light scattering laser power that is used to make DLS measurements. The laser level is optimized at run time by the instrument software when AutomaticDynamicLightScatteringLaserSettings is True and LaserLevel is Null.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: GreaterP[0 Percent]
DynamicLightScatteringDiode Attenuation
The percent of scattered light signal that is allowed to reach the avalanche photodiode for DLS measurements. The attenuator level is optimized at run time by the instrument software when AutomaticDynamicLightScatteringLaserSettings is True and DiodeAttenuation is Null.
Format: Single
Class: Real
Unit: 1 percent
Programmatic Pattern: GreaterP[0 Percent]
InitialDynamicLightScattering Temperature
The temperature at which the particle DLS is measured before thermal ramping.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
InitialZAverageDiameter
The average hydrodynamic diameter of particles in solution for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nanometer]
InitialZAverageDiffusion Coefficient
Diffusion coefficeint calculated from the CorrelationCurve which is inversely proportional to particle size, for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Unit: 1 meter squared per second
Programmatic Pattern: Meter
Hold[GreaterEqualP[0 (Meter ------)]]
Second
InitialDiameterStandard Deviation
The standard deviation of the ZAverageDiameter for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nanometer]
InitialPolydispersityIndex
A measure of the heterogeneity of the calculated hydrodynamic diameters of particles in solution for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
InitialScatteredLight Intensity
The amount of light that reaches the scattered light detector in counts per second for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
InitialCorrelationCurve
A series of data points which describes the correlation between scattered light intensity over time for the sample for the DLS measurement before thermal ramping. This correlation typically decays from 1 to 0 over time.
Format: Single
Class: QuantityArray
Unit: ['1 microsecond', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Micro Second, ArbitraryUnit}]
InitialCorrelationCurveFit Variance
A measure of the fit of multiple exponential decay function to the measured CorrelationCurve for the DLS measurement before thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
InitialIntensityDistribution
Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution for the DLS measurement before thermal ramping. Since larger particles scatter more light, larger particles have a higher scattered light intensity per particle in the IntensityDistribution.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]
InitialMassDistribution
Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution for the DLS measurement before thermal ramping. In the MassDistribution, as opposed to the IntensityDistribution, the scattered light intensity at each hydrodynamic diameter is corrected for particle size, so this trace gives a more accurate representation of the number of particles of each size in solution.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]
FinalDynamicLightScattering Temperature
The temperature at which the particle DLS is measured after thermal ramping.
Format: Single
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Celsius]
FinalZAverageDiameter
The average hydrodynamic diameter of particles in solution for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nanometer]
FinalZAverageDiffusion Coefficient
Diffusion coefficeint calculated from the CorrelationCurve which is inversely proportional to particle size, for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Unit: 1 meter squared per second
Programmatic Pattern: Meter
Hold[GreaterEqualP[0 (Meter ------)]]
Second
FinalDiameterStandard Deviation
The standard deviation of the ZAverageDiameter for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Unit: 1 nanometer
Programmatic Pattern: GreaterEqualP[0 Nanometer]
FinalPolydispersityIndex
A measure of the heterogeneity of the calculated hydrodynamic diameters of particles in solution for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
FinalScatteredLightIntensity
The amount of light that reaches the scattered light detector in counts per second for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
FinalCorrelationCurve
A series of data points which describes the correlation between scattered light intensity over time for the sample for the DLS measurement after thermal ramping. This correlation typically decays from 1 to 0 over time.
Format: Single
Class: QuantityArray
Unit: ['1 microsecond', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Micro Second, ArbitraryUnit}]
FinalCorrelationCurveFit Variance
A measure of the fit of multiple exponential decay function to the measured CorrelationCurve for the DLS measurement after thermal ramping.
Format: Single
Class: Real
Programmatic Pattern: GreaterEqualP[0]
FinalIntensityDistribution
Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution for the DLS measurement after thermal ramping. Since larger particles scatter more light, larger particles have a higher scattered light intensity per particle in the IntensityDistribution.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]
FinalMassDistribution
Trace of the calculated scattered light intensities versus hydrodynamic diameters of particles in solution for the DLS measurement after thermal ramping. In the MassDistribution, as opposed to the IntensityDistribution, the scattered light intensity at each hydrodynamic diameter is corrected for particle size, so this trace gives a more accurate representation of the number of particles of each size in solution.
Format: Single
Class: QuantityArray
Unit: ['1 nanometer', '1 ArbitraryUnits']
Programmatic Pattern: QuantityCoordinatesP[{Nanometer, ArbitraryUnit}]
Data Processing
RawDataFiles
The file(s) containing the raw unprocessed data generated by the instrument.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
DataFiles
The instrument software specific file(s) containing the processed data generated by the instrument.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[EmeraldCloudFile]
Analysis & Reports
MeltingAnalyses
Melting point analysis performed on this data.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
FluorescenceSpectraAnalyses
Fluorescence spectra analysis performed on this data (and its replicates).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
AggregationAnalyses
Aggregation temperature analyses done on this data (and its replicates).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
ThermalShiftAnalyses
Thermal shift analyses relative to a reference sample done on this data (and its replicates).
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
InitialIntensityPeaksAnalyses
Peak picking analyses performed on the InitialIntensityDistribution.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
FinalIntensityPeaksAnalyses
Peak picking analyses performed on the FinalIntensityDistribution.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
InitialMassPeaksAnalyses
Peak picking analyses performed on the InitialMassDistribution.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
FinalMassPeaksAnalyses
Peak picking analyses performed on the FinalMassDistribution.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
DynamicLightScattering Analyses
Analyses of the initial and final dynamic light scattering correlation curves.
Format: Multiple
Class: Link
Programmatic Pattern: _Link
Relation: Object[Analysis][Reference]
MeltingTemperature
The temperature at the first inflection point in the melting or cooling curve for each cycle. Additional melt temperatures can be found in the corresponding analysis object.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
AggregationTemperature
The temperature at the inflection point of the aggregation curve for each heating cycle or cooling cycle.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
AggregationOnsetTemperature
The temperature at the AggregationThreshold for each heating cycle or cooling cycle.
Format: Multiple
Class: Real
Unit: 1 degree Celsius
Programmatic Pattern: GreaterP[0 Kelvin]
Last modified on Mon 26 Sep 2022 15:43:44