ecl-logo Documentation

Object[Data, PAGE]

Data from separation of nucleic acids or proteins as determined by electrochemical motility, obtained by running Polyacrylamide Gel Electrophoresis (PAGE).

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, PAGE, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, PAGE]
    Pattern Description: Object[Data, PAGE]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

General

Experimental Results

    LowExposureLaneIntensity

    The distance down the lane vs. pixel intensity for the given lane image of the shortest exposure time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 millimeter', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]

    MediumLowExposureLane Intensity

    The distance down the lane vs. pixel intensity for the given lane image of the second shortest exposure time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 millimeter', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]

    MediumHighExposureLane Intensity

    The distance down the lane vs. pixel intensity for the given lane image of the second longest exposure time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 millimeter', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]

    HighExposureLaneIntensity

    The distance down the lane vs. pixel intensity for the given lane image of the longest exposure time.
    Format: Single
    Class: QuantityArray
    Unit: ['1 millimeter', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]

    OptimalLaneIntensity

    The distance down the lane vs. pixel intensity for the given lane image.
    Format: Single
    Class: QuantityArray
    Unit: ['1 millimeter', '1 ArbitraryUnits']
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]

    LowExposureLadderIntensity

    The distance down the lane vs. pixel intensity for this analyte's corresponding ladder lane of the shortest exposure time.
    Format: Computable
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], LowExposureLaneIntensity]]

    MediumLowExposureLadder Intensity

    The distance down the lane vs. pixel intensity for this analyte's corresponding ladder lane of the second shortest exposure time.
    Format: Computable
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumLowExposureLaneIntensity]]

    MediumHighExposureLadder Intensity

    The distance down the lane vs. pixel intensity for this analyte's corresponding ladder lane of the second longest exposure time.
    Format: Computable
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumHighExposureLaneIntensity]]

    HighExposureLadderIntensity

    The distance down the lane vs. pixel intensity for this analyte's corresponding ladder lane of the longest exposure time.
    Format: Computable
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], HighExposureLaneIntensity]]

    OptimalLadderIntensity

    The distance down the lane vs. pixel intensity for this analyte's corresponding ladder lane.
    Format: Computable
    Programmatic Pattern: QuantityArrayP[{{Milli Meter, ArbitraryUnit}..}]
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], OptimalLaneIntensity]]

    LowExposureLaneImage

    Photographic image of the lane in the gel for the shortest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LowExposureLaneImageFile]}, ImportCloudFile[Field[LowExposureLaneImageFile]]]

    MediumLowExposureLaneImage

    Photographic image of the lane in the gel for the second shortest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[MediumLowExposureLaneImageFile]}, ImportCloudFile[Field[MediumLowExposureLaneImageFile]]]

    MediumHighExposureLaneImage

    Photographic image of the lane in the gel for the second longest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[MediumHighExposureLaneImageFile]}, ImportCloudFile[Field[MediumHighExposureLaneImageFile]]]

    HighExposureLaneImage

    Photographic image of the lane in the gel for the longest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[HighExposureLaneImageFile]}, ImportCloudFile[Field[HighExposureLaneImageFile]]]

    OptimalLaneImage

    Photographic image of the lane in the gel.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[OptimalLaneImageFile]}, ImportCloudFile[Field[OptimalLaneImageFile]]]

    LowExposureLadderImage

    Photographic image of this analyte's corresponding ladder lane for the shortest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], LowExposureLaneImage]]

    MediumLowExposureLadderImage

    Photographic image of this analyte's corresponding ladder lane for the second shortest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumLowExposureLaneImage]]

    MediumHighExposureLadderImage

    Photographic image of this analyte's corresponding ladder lane for the second longest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumHighExposureLaneImage]]

    HighExposureLadderImage

    Photographic image of this analyte's corresponding ladder lane for the longest exposure time.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], HighExposureLaneImage]]

    OptimalLadderImage

    Photographic image of this analyte's corresponding ladder lane.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], OptimalLaneImage]]

    LowExposureLaneImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MediumLowExposureLaneImage File

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MediumHighExposureLaneImage File

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    HighExposureLaneImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    OptimalLaneImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    LowExposureLadderImageFile

    File containing a photographic image of this analyte's coresponding ladder lane for the shortest exposure time.
    Format: Computable
    Programmatic Pattern: ImageFileP
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], LowExposureLaneImageFile]]

    MediumLowExposureLadderImage File

    File containing a photographic image of this analyte's coresponding ladder lane for the second shortest exposure time.
    Format: Computable
    Programmatic Pattern: ImageFileP
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumLowExposureLaneImageFile]]

    MediumHighExposureLadderImage File

    File containing a photographic image of this analyte's coresponding ladder lane for the second longest exposure time.
    Format: Computable
    Programmatic Pattern: ImageFileP
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], MediumHighExposureLaneImageFile]]

    HighExposureLadderImageFile

    File containing a photographic image of this analyte's coresponding ladder lane for the longest exposure time.
    Format: Computable
    Programmatic Pattern: ImageFileP
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], HighExposureLaneImageFile]]

    OptimalLadderImageFile

    File containing a photographic image of this analyte's coresponding ladder lane.
    Format: Computable
    Programmatic Pattern: ImageFileP
    Expression: SafeEvaluate[{Field[LadderData]}, Download[Field[LadderData], OptimalLaneImageFile]]

Analysis & Reports

    DataType

    Indciates if this data represents a standard or an analyte sample.
    Format: Single
    Class: Expression
    Programmatic Pattern: PAGEDataTypeP

    LadderData

    Data containing the ladder run alongside with this analyte.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    Analytes

    The samples, or analytes, that were run on a polyacrylamide gel in this PAGE experiment alongside with this ladder.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    LadderSizes

    List of sizes of the fragments in the provided ladder.
    Format: Computable
    Programmatic Pattern: {GreaterEqualP[0, 1]..}
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Download[Field[LadderAnalyses], Sizes]]

    LadderAnalyses

    A link to the ladder analysis object for this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    LadderPeaks

    List of the position of the ladder peaks.
    Format: Computable
    Programmatic Pattern: {(GreaterEqualP[0, 1] -> GreaterP[0])..}
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Computables`Private`ladderPeaks[Field[LadderAnalyses]]]

    SizeFunction

    Pure function which takes a retention time as input and returns an expected size as output (as interpolated by the ladder fit).
    Format: Computable
    Programmatic Pattern: _Function
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Computables`Private`sizeFunction[Field[LadderAnalyses]]]

    RetentionFunction

    Pure function which takes a fragment size as input and returns an expected retention time as output (as interpolated by the ladder fit).
    Format: Computable
    Programmatic Pattern: _Function
    Expression: SafeEvaluate[{Field[LadderAnalyses]}, Computables`Private`retentionFunction[Field[LadderAnalyses]]]

    LanePeaksAnalyses

    Peak-picking analyses conducted on this lane image.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    SmoothingAnalyses

    Smoothing analysis performed on this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

Data Processing

    LowExposureGelImage

    Full sized uncropped image of the entire gel, including all lanes.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[LowExposureGelImageFile]}, ImportCloudFile[Field[LowExposureGelImageFile]]]

    MediumLowExposureGelImage

    Full sized uncropped image of the entire gel, including all lanes.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[MediumLowExposureGelImageFile]}, ImportCloudFile[Field[MediumLowExposureGelImageFile]]]

    MediumHighExposureGelImage

    Full sized uncropped image of the entire gel, including all lanes.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[MediumHighExposureGelImageFile]}, ImportCloudFile[Field[MediumHighExposureGelImageFile]]]

    HighExposureGelImage

    Full sized uncropped image of the entire gel, including all lanes.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[HighExposureGelImageFile]}, ImportCloudFile[Field[HighExposureGelImageFile]]]

    OptimalGelImage

    Full sized uncropped image of the entire gel, including all lanes.
    Format: Computable
    Programmatic Pattern: _Image
    Expression: SafeEvaluate[{Field[OptimalGelImageFile]}, ImportCloudFile[Field[OptimalGelImageFile]]]

    LowExposureGelImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MediumLowExposureGelImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    MediumHighExposureGelImage File

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    HighExposureGelImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    OptimalGelImageFile

    File containing the full sized uncropped image of the entire gel, including all lanes.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link

    InvertIntensity

    Indicate if the gel image has been inverted for intensity calculations.
    Format: Single
    Class: Expression
    Programmatic Pattern: BooleanP

    LaneNumber

    The lane index starting from the left most lane of the gel.
    Format: Single
    Class: Integer
    Programmatic Pattern: GreaterP[0, 1]

    NeighboringLanes

    Data from analytes or ladders that were run on the same gel.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link

    Scale

    The scale in pixels/distance relating pixels of the darkroom image to real world distance.
    Format: Single
    Class: Real
    Unit: 1 Pixels per centimeter
    Programmatic Pattern: 0 Pixel Hold[GreaterEqualP[-----------]] Centi Meter
Last modified on Mon 26 Sep 2022 15:43:44