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Object[Data, qPCR]

Quantitative Polymerase Chain Reaction (sometimes called Real-Time Polymerase Chain Reaction) measurements of fluorescence vs number of thermal cycles.

Organizational Information

    Name

    Name of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: A string.

    ID

    ID of this Object.
    Format: Single
    Class: String
    Programmatic Pattern: _String
    Pattern Description: The ID of this object.

    Object

    Object of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, qPCR, _String]
    Pattern Description: The object reference of this object.

    Type

    Type of this Object.
    Format: Single
    Class: Expression
    Programmatic Pattern: Object[Data, qPCR]
    Pattern Description: Object[Data, qPCR]

    Notebook

    Notebook this object belongs to.
    Format: Single
    Class: Link
    Programmatic Pattern: _Link
    Pattern Description: An object of that matches ObjectP[Object[LaboratoryNotebook]].
    Relation: Object[LaboratoryNotebook][Objects]

    Replicates

    Data acquired under replicated experimental conditions to this data.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Data][Replicates]

General

Data Processing

    AmplificationCurveTypes

    For each member of EmissionWavelengths, the category of amplification curve data. Examples include PrimaryAmplicon, EndogenousControl, PassiveReference, and Unused.
    Format: Multiple
    Class: Expression
    Programmatic Pattern: qPCRCurveTypeP

    RawAmplificationCurves

    For each member of EmissionWavelengths, the raw (non normalized) curve of fluorescence vs cycle as measured at each cycle of PCR amplification (only populated if amplification curves are normalized to a passive reference dye).
    Format: Multiple
    Class: QuantityArray
    Unit: ['None', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{None, RFU}]

    RawMeltingCurves

    For each member of EmissionWavelengths, the raw (non normalized) curve of fluorescence vs temperature as measured during a temperature sweep peformed after PCR amplification is complete (only populated if melting curves are normalized to a passive reference dye).
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 degree Celsius', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Celsius, RFU}]

Experimental Results

    AmplificationCurves

    For each member of EmissionWavelengths, the curve of fluorescence vs cycle as measured at each cycle of PCR amplification, normalized to the passive reference amplification curve if available.
    Format: Multiple
    Class: QuantityArray
    Unit: ['None', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{None, RFU}]

    AmplificationReadTemperature

    The PCR block temperature at the time of fluorescence data collection during each cycle of PCR amplification.
    Format: Single
    Class: QuantityArray
    Unit: ['None', '1 degree Celsius']
    Programmatic Pattern: QuantityCoordinatesP[{None, Celsius}]

    MeltingCurves

    For each member of EmissionWavelengths, the curve of fluorescence vs temperature as measured during a temperature sweep peformed after PCR amplification is complete, normalized to the passive reference melting curve if available. When using only one primer pair, this curve can be used to assess whether the primers produced a single, specific amplicon.
    Format: Multiple
    Class: QuantityArray
    Unit: ['1 degree Celsius', '1 Rfus']
    Programmatic Pattern: QuantityCoordinatesP[{Celsius, RFU}]

Analysis & Reports

    QuantificationCycleAnalyses

    The analysis object(s) containing the quantification cycle calculation results.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis][Reference]

    CopyNumberAnalyses

    The analysis object(s) containing the copy number calculation results. Each copy number is determined from a standard curve of quantification cycle vs Log10 copy number.
    Format: Multiple
    Class: Link
    Programmatic Pattern: _Link
    Relation: Object[Analysis, CopyNumber][Data]
Last modified on Mon 26 Sep 2022 15:43:44
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